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ANS 3319C Reproduction and Endocrinology of Farm Animals

Histology Lab

Prepared by Adriane Bell and Lilian Oliveira

Human Ovary Note: egg nest with primordial follicles

Bovine Ovary Note: primary follicles with single layer of granulosa cells

Bovine ovary

Note: primary follicles with single layer of granulosa cells

Bovine ovary Secondary follicle ­ two layers

Bovine graafian follicle (tertiary)

Note: oocyte, antrum, granulosa cells, and theca externa and interna.

Human Ovary ­ Please note granulosa cells around follicle antrum

Bovine Isthmus

Bovine Isthmus Note: greater amounts of smooth muscle and fewer folds compared to ampulla.

Bovine ampulla Note: Large numbers of folds and decreased smooth muscle compared to the isthmus.

Bovine ampulla

Bovine ampulla Note: Ciliated columnar epithelium

Bovine endometrium: uterine glands Note columnar epithelium composing the glands.

Bovine endometrium Site of prostaglandinF2 production

Bovine cervix

Note: crypts and folds, connective tissue and small amounts of smooth muscle underneath the epithelium

Bovine cervix Note crypts and folds, connective tissue and small amounts of smooth muscle underneath the epithelium

Bovine cervix: Note: crypts and folds, connective tissue and small amounts of smooth muscle underneath the epithelium Also, note columnar with some glandular epithelium that secrete mucous.

Cervix ­ Please note crypts and folds, connective tissue and small amounts of smooth muscle underneath the epithelium Also, note columnar with some glandular epithelium that secrete mucous.

Bovine vagina Note: stratified squamous epithelium for strength

Bovine vagina Note: stratified squamous epithelium for strength

Bovine epididymis (caput or head) Ciliated pseudostratified columnar for sperm movement and absorption of fluid

Bovine epididymis (caput or head) Remember ­ only one tube, many twists and turns Dark mass within tube is sperm.

Bovine epididymis (cauda or tail) Note: larger tube diameter and dark mass within tube is sperm.

Bovine epididymis (cauda or tail) Note: larger tube diameter and dark mass within tube is sperm.

Equine Testes (Normal) Seminiferous tubules

Equine Testes (Normal) Seminiferous tubules

Equine Testes (Normal) Seminiferous tubules and leydig cells

Equine Testes (Cryptorchid) Note: seminiferous tubules are smaller in diameter and lack of sperm cells in tubules

Equine Testes (Cryptorchid) Seminiferous tubules

Equine Testes (Normal) Seminiferous tubules

Bovine prostate gland

Bovine prostate gland Note: cuboidal cells for secretion of fluid to add volume

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