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Type of bioreactor and design

Polson Mahakhan; MIC431 Bioreactors

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reactor design

· Weizman 1941-1918

­ Clostridium acetobutylicum -> acetone ­ anaerobic

· 1930-1940 Compressed yeast

­ Aerobic

· 1943 Penicillin -> submerged culture

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· · , · · pH · · ·

· ·

· · · , ·

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Polson Mahakhan; MIC431 Bioreactors

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·

­ ; ­ stainless stell ­ ­ phenolic epoxy ­ ­

· , , , ()

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· Laboratory scale fermenter · Pilot-plant scale fermenter · Factory scale fermenter

­ 20,000, 50,000, 500,000, 2,500,000 L

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­ 1-20 L, working volume ½-15 L ­ 40-200 L -> 1000 L

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· (aeration) · (agitation) · (temperature cont.) · - (pH cont.) · (oxygent cont.) · (antifoam control)

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Polson Mahakhan; MIC431 Bioreactors

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Polson Mahakhan; MIC431 Bioreactors

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· Packed tower · Tower fermenter · Waldhof-type fermenter · Acetator & cavitator · Cyclone column · Cylindro-conical vessel · Air-lift fermenter · Deep-jet fermenter · Rotating disc fermenter

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Packed tower · ·

· , · · Generator · , · , ,

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­ , , , , polystyrene

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Tower fermenter

() · tubular section 6:1 or total 10:1 · · batch continuous · · Besik 1973

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Tower fermenter

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Waldhof-type fermenter

· yeast sulphite waste liqour · rotating pin-wheel ·

­ , ­

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Waldhof-type fermenter

Rotating pin-wheel

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Acetator

· Frings generator · Heindrich Frings · Acetobacter · · self-priming aerator ·

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Frings generator Mahakhan; MIC431 Polson

Bioreactors

Self-priming aerator

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Cavitator

· · ·

· acetator

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Cyclone column

· · · ·

· ·

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Cylindro-conical vessel

· Nathan 1930 · 1960 · · 22-25 m 6 m 450,000 · 40-48 h, · ·

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Cylindroconical vessel

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Air-lift fermenter

· single cell protein

methanol · · Air-lift 45 m

­ Outer loop < inner loop ­

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Air-lift fermenter

Outer loop

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Inner loop

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Deep-jet fermenter

· · air entrainer

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Rotating disc fermenter

· · disc · Anderson & Blain 1980

­ Polypropylene disc, 40 L ­ Aspergillus, Rhizopus, Mucor, ­ citric acid A. niger, 80g/l

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Penicillium

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Type of bioreactor and design

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