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CinnaGen

Introduction

Being more than 10 years in the Iranian market and cover more than 80% of the national needs in area of Molecular biology, genetic and biotechnology, CinnaGen Company is proud to be internationally recognized with its high quality products and brand names in more than 22 countries in the five continents. By the rapid progress of biotechnology in Iran in the last 10 years, The Islamic Republic of Iran was positioned at the 5th top ranking country after Japan, South Korea, China and India. For sure the share of CinnaGen as a trustable supplier to the scientific community of Iran is something that can not be ignored. In the last years, CinnaGen has tried to move on to built the national capacity by helding more than 45 E-workshops and in site training in the country. In 2006, 21 workshops in different areas of biotechnology were held in collaboration with the Iranian universities and research centers and support of High-Tech department of the Ministry of Industry of Iran. Our aim in CinnaGen is not only to supply the reagents but also to be as a partner and consultant to the scientific community. Technical supports and fast delivery system for the clients are our strategy. Now Oligo synthesis is available with 72 hrs delivery time for local and 5 working days for international clients. High quality products and full dedication to customer satisfaction always make CinnaGen unique among her competitors. Research and development in area of recombinant biopharmaceutical products puts CinnaGen Company as the second producer of Interferon beta-1a in the lyophilized form, CinnoVexTM, after Biogen-Idec, USA in the world. Erythropoietin and two more recombinant drugs are in the pipeline for production. It is our honor to come along with the scientific community in order to serve researchers and promote the scientific activities for better life.

Dear reliable customers Please find both nine Numerical code and catalog Number for each selected item then send your enquiry by fax or e-mail as below: Fax: +9821 4466 81 77 E-mail: [email protected] You can send your questions about CinnaGen catalog by [email protected] Bulk order is also available on request.

Contents

Restriction Enzymes ................................. DNA/RNA Polymerases PCR Systems

1 2 3 4 5 6 7 8 9 10 11 12 13 14

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Molecular Cloning

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Nucleic Acid Purification ......................... In Vitro Transcription .............................. Protein / DNA Ladders & Markers ........... Nucleotides

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PCR Detection Kits

...............................

Clinical Chemistry Kit Blood Group Typing Cell Culture

...........................

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ELISA Based Diagnostic Kits ................... Generic Peptides

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Alphabetical Index

Restriction Enzymes:

AluI 5'-A G^C T-3' ER0011 ER0012 10u/µl AvaII (Eco47I ) T 5'-G^G A C C-3' ER0311 ER0312 10u/µl BamHI 5'-G^G A T C C-3' ER0051 ER0052 ER0053 FD0054 BamHI BclI 5'-T^G A T C A-3' ER0721 ER0722 10u/µl BglI 5'-G C C N N N N^N G G C-3' ER0071 2000u 316502034 1000u 5000u 316402028 316602028 4000u 5x4000u HC, 20000u 200 reactions, FastDigestTM 10u/µl, 50u/µl, HC 316602025 316702025 316802025 351302025 800u 4000u 316402114 316602114 600u 3000u 316402018 316602018

ER0072 10u/µl BglII 5'-A^G A T C T-3' ER0081 ER0082 10u/µl DraI (AhaIII) 5'-T T T^A A A-3' RE7705C ER0221 ER0222 ER0223 FD0224 10u/µl, 50u/µl, HC EcoRI

5x2000u

316702034

1

500u 2500u

316302035 316502035

1500u 1500u 5x1500u HC, 7500u 200 reactions, FastDigestTM

616501103 316502103 316602103 316702103 351302013

5'-G^A A T T C-3' RE7707C ER0271 ER0272 ER0273 FD0274 10u/µl; 50u/µl, HC (EcoRV) (Eco32I) 5'-G A T^A T C-3' ER0301 2000u 316502113 5 2500u 5000u 5x5000u HC,25000u 200 reactions, FastDigestTM 616501124 316502124 316702124 316802124 351202113

ER0302 ER0303 FD0304 10u/µl; 50u/µl, HC (HaeIII) BsuRI 5'-G G^C C-3' ER0151 ER0152 10u/µl HhaI (Hin6I) 5'-G^C G C-3' ER0481 ER0482 10u/µl HincII (HindII)

5x2000u HC,10000u 100 reactions , FastDigestTM

316602113 316702113 351202113

3000u 5x3000u

316602072 316802072

2000u 5x2000u

316502151 316702151

5'-G T Py^Pu A C-3' ER0491 ER0492 FD0494 HincII HindIII 5'-A^A G C T T-3' RE7709C ER0501 ER0502 ER0503 2500U 5000u 5x5000u HC, 25000u, 616501153 316602153 316702153 316802153 500u 2500u 50 reactions, FastDigestTM 10u/µl 316302152 316502152 351202152

FD0504 10u/µl; 50u/µl, HC HinfI 5'-G^A N T C-3' RE7710C ER0801 ER0802 ER0803 10u/µl; 50u/µl, HC KpnI

200 reactions, FastDigestTM

351302153

1

2000U 2000u 5x2000u HC, 10000u

616501154 316502154 316702154 316802154

5'-G G T A C^C-3' ER0521 ER0522 ER0523 FD0524 10u/µl; 50u/µl, HC MboI 5'-^G A T C -3' ER0811 ER0812 10u/µl MspI (HpaII) 5'-C^C G G-3' ER0541 ER0542 10u/µl 7 3000u 5x3000u 316602192 316802192 300u 1500u 316302186 316502186 4000u 5x4000u HC, 20000u 100 reactions , FastDigestTM 316502161 316702161 316802161 351202161

NcoI 5'-C^C A T G G-3' ER0571 ER0572 FD0574 10u/µl NdeI 5'-C A^T A T G-3' ER0581 ER0582 FD0584 10u/µl NotI 5'-G C^G G C C G C-3' ER0591 ER0592 ER0593 FD0594 300u 1500u HC, 1500u 50 reactions, FastDigest

TM

500u 2500u 50 reactions, FastDigest

TM

316302199 316502199 351202199

500u 2500u 50 reactions, FastDigest

TM

316302200 316502200 351202200

316302203 316402203 316502203 351202203

10u/µl; 30-50u/µl, HC PstI 5'-C T G C A^G-3' RE7613C ER0611 ER0612 ER0613 FD0614 10u/µl; 50u/µl, HC 3000u 3000u 5x3000u HC,15000u 200 reactions, FastDigest

TM

616601239 316602239 316702239 316902239 351302239

PvuI 5'-C G A T^C G-3' ER0621 ER0622 FD0624 10u/µl PvuII 5'-C A G^C T G-3' RE7724C ER0631 ER0632 ER0633 FD0634 10u/µl; 50u/µl, HC RsaI 5'-G T^A C-3' ER1121 ER1122 FD1124 10u/µl SacI 5'-G A G C T^C-3' ER1131 ER1132 ER1133 FD1134 10u/µl; 50u/µl, HC 9 1200u 5x1200u HC,6000u 50 reactions, FastDigestTM 316502259 316602259 316702259 351202259 1000u 5000u 50 reactions, FastDigestTM 316402258 316602258 351202258 2500u 2500u 5x2500u HC, 12500u 200 reactions, FastDigestTM 616501251 316502251 316702251 316802251 351302251 300u 1500u 50 reactions, FastDigestTM 316302250 316502250 351202250

1

SalI 5'-G^T C G A C-3' RE7715C ER0641 ER0642 ER0643 FD0644 10u/µl; 50u/µl, HC SmaI 5'-C C C^G G G-3' ER0661 ER0662 ER0663 FD0664 10u/µl; 50u/µl, HC TaqI 5'-T^C G A-3' RE7717C ER0671 ER0672 ER0673 FD0674 10u/µl,50u/µl, HC XbaI 5'-T^C T A G A-3' ER0681 ER0682 1500u 5x1500u 316502291 3000u 3000u 5x3000u HC, 15000u 200 reactions, FastDigestTM 616601279 316602279 316702279 316802279 351302279 1200u 5x1200u HC, 6000u 50 reactions, FastDigestTM 316502267 316602267 316702267 351202267 1500u 1500u 5x1500u HC, 7500u 100 reactions, FastDigestTM 616501260 316502260 316602260 316702260 351202260

ER0683 FD0684 10u/µl; 50u/µl, HC XhoI

HC, 7500u 200 reactions, FastDigestTM

316602291 316702291 351302291

1

5'-C^T C G A G-3' ER0691 ER0692 ER0693 FD0694 10u/µl; 50u/µl, HC 2000u 5x2000u HC, 10000u 200 reactions, FastDigestTM 316502474 316602474 316702474 351302474

FastDigestTM Restriction Enzymes

Digestion of DNA with restriction enzymes is a time consuming step in cloning and clone analysis. DNA digestions typically last for one hour, and often DNA is incubated with the enzymes overnight. Fermentas FastDigestTM Restriction Enzymes specifically formulated to cleave DNA in just 5 minutes. High quality DNA is crucial for efficient DNA digestion in 5 minutes.

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2 DNA/RNA Polymerases

DNA/RNA Polymerases

Thermophilic DNA Polymerase:

Hot Start Taq DNA Polymerase EP0601 EP0602 100u (5u/µl) 500u (5u/µl) 352202417 352302417

2

Hot Start Taq DNA Polymerase is designed for hot-start PCR, a technique that enhances the specificity, sensitivity and yield of DNA amplification. In addition, the enzyme provides the convenience of reaction set-up at room temperature. Hot Start Taq DNA Polymerase is a recombinant Taq DNA polymerase which has been chemically modified by the addition of heat-labile blocking groups to its amino acid residues. Applications: High yield amplification of targets up to 3 kb., Hot-start PCR, RT-PCR, Highly specific amplification of complex genomic and cDNA templates, Amplification of low copy DNA targets, Multiplex PCR, Generation of PCR products for TA cloning and Real-time PCR. Pfu DNA Polymerase (Recombinant) EP0501 EP0502 100u, 2.5u/µl 500u, 2.5u/µl 616201459 616301459

The Pfu DNA Polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeum Pyrococcus furiosus. The Pfu DNA Polymerase also exhibits 3'=>5' exonuclease (proofreading) activity, that enables the polymerase to correct nucleotide incorporation errors. It has no 5'=>3' exonuclease activity. Source: Pfu DNA Polymerase (recombinant) - E.coli cells with a cloned pol gene from Pyrococcus furiosus. SmarTaq DNA Polymerase (Recombinant) TA8108C TA8109C TA8110C 100u (5 u/µl) 500u (5 u/µl) 5000u (5 u/µl) 616201414 616301414 616601414

Supplied with: 10XPCR Buffer 100mM Tris-HCl (pH 8.8 at 25°C), 500mM KCl , 10X PCR 13

Buffer (with 750mM Tris-HCl (pH 8.8 at 25°C), 200mM (NH4)2SO4, 0.1% Tween 20 and 50mM MgCl2.

AMS (Ammonium Sulfate) 10X PCR Buffer is not included. It can be provided free of charge upon request with any purchase.

2

Taq DNA Polymerase (Recombinant) TA7505C TA7506C TA7507C 50mM MgCl2. The Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. Source : E.coli cells with a cloned gene from Thermus aquaticus. Taq DNA Polymerase (Recombinant) EP0401 EP0402 100u (5u/µl) 500u (5u/µl) 316202418 316302418 100U(5 u/µl) 500U(5 u/µl) 5000U(5 u/µl) 616201417 616301417 616601417

Supplied with: 10XPCR Buffer 100mM Tris-HCl (pH 8.8 at 25°C), 500mM KCl and

The Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. Source: E.coli cells with a cloned gene from Thermus aquaticus. Taq DNA Polymerase (native, Without BSA) EP0281 EP0282 100u (5u/µl) 500u (5u/µl) 316202418 316302418

The Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. Source: Thermus aquaticus YT1 cells.

14

Mesophilic DNA Polymerase:

DNA Polymerase I, E.coli EP0041 EP0042 500u (10u/µl) 2500u (10u/µl) 316302892 316502892

2

The DNA Polymerase I, a template-dependent DNA polymerase, catalyzes 5'=>3' synthesis of DNA. The enzyme also exhibits 3'=>5' exonuclease (proofreading) activity, 5'=>3' exonuclease activity and ribonuclease H activity. Source: E.coli cells with a cloned polA gene. Klenow Fragment EP0051 EP0052 EP0054 300u (10u/µl) 1500u (10u/µl) LC,300u (2u/µl) 316302359 300502359 300302359

The Klenow Fragment is the Large Fragment of DNA Polymerase I, E.coli. It exhibits 5'=>3' polymerase activity and 3'=>5' exonuclease (proofreading) activity, but lacks 5'=>3' exonuclease activity of DNA polymerase I. Source: E.coli cells with a cloned fragment of the polA gene. Klenow Fragment, exoEP0421 EP0422 300u (5u/µl) 1500u (5u/µl) 316402359 316502359

The Klenow Fragment, exo-, is the Large Fragment of DNA Polymerase I, E.coli. It exhibits 5'=>3' polymerase activity, but lacks the 3'=>5' and 5'=>3' exonuclease activities of DNA Polymerase I. The 3'=>5' exonuclease activity of the enzyme is eliminated by mutations in the 3'=>5'-exonuclease active site. Source: ,E.coli cells with a cloned DNA fragment of the mutated polA gen.

15

T4 DNA Polymerase EP0061 EP0062 100u (5u/µl) 500u (5u/µl) 316302357 316402357

2

The T4 DNA Polymerase, a template-dependent DNA polymerase, catalyzes 5'=>3' synthesis from primed single-stranded DNA. The enzyme has a 3'=>5' exonuclease activity, but lacks 5'=>3' exonuclease activity. Source: E.coli cells with a cloned gene 43 of bacteriophage T4. T7 DNA Polymerase EP0081 300u (10u/µl) 316302612 The T7 DNA Polymerase, a template dependent DNA polymerase, catalyzes DNA synthesis in the 5'=>3' direction. It is a highly processive DNA polymerase allowing continuous synthesis of long stretches of DNA. The enzyme also exhibits a high 3'=>5' exonuclease activity towards single- and double-stranded DNA. Source: Two E.coli strains, one with the cloned gene 5 of bacteriophage T7, and the other with the cloned trxA gene of E.coli.

Reverse Transcriptase M-MuLV Reverse Transcriptase

RevertAidTM M-MuLV Reverse Transcriptase EP0441 EP0442 10,000u (200u/µl) 5x10,000u (200u/µl) 316702412 316802412

The RevertAidTM M-MuLV Reverse Transcriptase (RT) is a genetically modified MMuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a ribonuclease H activity specific to RNA in RNA-DNA hybrids. Source: E.coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus reverse transcriptase.

16

RNA Polymerases

Ribonuclease Inhibitors RiboLockTM Ribonuclease Inhibitor EO0381 EO0382 RiboLock

TM

2500u (40u/µl) 4x2500u (40u/µl)

316502967 316702967

2

Ribonuclease Inhibitor inhibits the activity of RNases A, B and C by binding

them in a noncompetitive mode at a ratio 1:1. It does not inhibit RNases 1, T1, T2, H, U1, U2, CL3 or other enzymes. Source: E.coli cells carrying a cloned gene encoding mammalian ribonuclease inhibitor. It protects RNA from degradation over temperature range of 25-55°C, some activity is still measured at 60°C T7 RNA Polymerase EP0111 EP0112 EP0113 5000u (20u/µl) 5x5000u (20u/µl) HC, 25,000u (>100u/µl) 316602947 316702947 316802947

Bacteriophage T7 RNA polymerases are DNA-dependent RNA polymerases with strict specificity for their respective double-stranded promoters. They catalyze the 5'=>3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from their promoters. Source: E.coli cells with a cloned gene encoding the corresponding RNA polymerase.

Nucleases

Deoxyribonuclease I (DNase I), RNase-free EN0521 EN0523 EN0525 1000u (1u/µl) HC, 1000u (50u/µl) 1000u (1u/µl) 316502379 316602379 316402379

Deoxyribonuclease I (DNase I), RNase-free is an endonuclease that digests single and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono and 17

oligodeoxyribonucleotides with 5-phosphate and 3'-OH groups. The enzyme activity is strictly dependent on Ca2+ and is activated by Mg2+ and Mn2+ ions.

2

Ribonucleases: Ribonucleases (RNases)

Ribonuclease A (RNase A), DNase-free EN0531 10mg (10mg/ml) 316002408 Ribonuclease A (RNase A), DNase-free is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues. It cleaves the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide. The resulting 2', 3'-cyclic phosphate may be hydrolyzed to the corresponding 3'-nucleoside phosphate. Source: Bovine pancreas. Ribonuclease T1 EN0541 EN0542 100,000u (1000u/µl) 500,000u (1000u/µl) 316802767 316902767

Ribonuclease T1 (RNase T1) is an endoribonuclease that specifically degrades singlestranded RNA at G residues. It cleaves the phosphodiester bond between 3'-guanylic residues and the 5'-OH residues of adjacent nucleotides with the formation of the corresponding intermediate 2', 3'-cyclic phosphates (1). The reaction products are 3'GMP and oligonucleotides with a terminal 3'-GMP. Source: E.coli cells carrying a cloned rntA gene of Aspergillus oryzae. RNase A/T1 Mix EN0551 1ml 316102408 (2mg/ml of RNase A and 5000u/ml of RNase T1)

RNase A/T1 Mix combines the RNA degradation activity of both RNase A and RNase T1. RNase A specifically hydrolyzes RNA at C and U residues; RNase T1 specifically hydrolyzes RNA at G residues. Source: RNase A: Bovine pancreas, RNase T1: E.coli cells carrying a cloned rntA gene of Aspergillus oryzae. 18

Ligases

T4 DNA Ligases EL0014 EL0011 T4 DNA Ligase (with PEG) EL0334 EL0331 200u (5u/µl) 1000u (5u/µl) 316102415 316602415 200u (5u/µl) 1000u (5u/µl) 316502415 316702415

2

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA with blunt or cohesiveend termini. The enzyme repairs single-strand nicks in duplex DNA, RNA or DNA/RNA hybrids but has no activity on single-stranded nucleic acids. Requires ATP as cofactor. Source: E.coli cells carrying a cloned gene 30 of bacteriophage T4. T4 RNA Ligase EL0021 1000u(10u/µl) 316402978 T4 RNA Ligase catalyzes the ATP-dependent intra- and intermolecular formation of phosphodiester bonds between 5'-phosphate and 3'-hydroxyl termini of polyand oligonucleotides, single-stranded RNA and DNA. The minimal substrate is a nucleoside 3',5'-biphosphate in intermolecular reaction and oligonucleotide of 8 bases in intramolecular reaction. Source: E.coli cells carrying a cloned gene 63 of bacteriophage T4.

Phosphatase and Kinase

Bacterial Alkaline Phosphatase EF0261 7500u (150u/µl) 316602881

Calf Intestine Alkaline Phosphatase EF0341 200u (1u/µl) 300002881 19

Shrimp Alkaline Phosphatase EF0511 500u (1u/µl) 316302881 Alkaline Phosphatase (SAP) catalyzes the release of 5'- and 3'-phosphate groups from

2

DNA, RNA, deoxyribonucleoside and ribonucleoside mono-, di- and triphosphates. Also this enzyme can remove phosphate groups from proteins.

Kinase

T4 Polynucleotide Kinase EK0031 EK0032 500u (10u/µl) 2500u (10u/µl) 316302416 316502416

T4 Polynucleotide Kinase (T4 PNK) is a polynucleotide 5'-hydroxyl kinase that catalyzes the transfer of the gama-phosphate from ATP to the 5'-OH group of single- and doublestranded DNAs and RNAs, oligonucleotides or nucleoside 3'-monophosphates (forward reaction). The reaction is reversible. In the presence of ADP, T4 PNK exhibits 5'-phosphatase activity and catalyzes the exchange of terminal 5'-phosphate groups (exchange reaction). The enzyme is also a 3'-phosphatase. Source: E.coli cells carrying a cloned pseT gene of bacteriophage T4.

Other enzymes

Agarase EO0461 100u (0.5u/µl) 316202874 Agarase specifically digests the agarose polysaccharide core made up of repeating 1,3linked beta-D-galactopyranose and 1,4-linked 3,6-anhydro-alfa-L-galactopyranose into neoagaro-oligosaccharides. Source: E.coli cells carrying a plasmid with a cloned gene encoding beta-agarase from Pseudomonas atlantica. DNA Topoisomerase I ET0211 200u (10u/µl) 316302988 DNA Topoisomerase I (Topo I) catalyzes nicking of supercoiled covalently closed circular 20

DNA and subsequent religation of the single-stranded break. Source:Calf thymus. Proteinase K EO0491 EO0492 1ml (~600u/ml, 20mg/ml) 5x1ml (~600u/ml, 20mg/ml) 316102407 316202407

2

Proteinase K is an endolytic protease that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic or hydrophobic amino acids. Proteinase K is classified as a serine protease. The smallest peptide to be hydrolyzed is a tetrapeptide. Source: Tritirachium album. Pyrophosphatase, Inorganic (from yeast) EF0221 10u (0.1u/µl) 316102944 Pyrophosphatase, Inorganic (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to two orthophosphates. The enzyme requires a divalent metal cation, with Mg2+ conferring the highest activity. Source: E.coli cells carrying a cloned ppa gene of Sacharomyces cerevisiae. Uracil-DNA Glycosylase EN0361 EN0362 200u (1u/µl) 5x200u (1u/µl) 316202038

Uracil-DNA Glycosylase catalyzes the hydrolysis of the N-glycosylic bond between the uracil and sugar, leaving an apyrimidinic site in uracil-containing single or doublestranded DNA. Shows no activity on RNA. Source: E.coli K12 cells.

21

3

PCR Systems

PCR Systems:

Hot Start Taq DNA Polymerase EP0601 EP0602 100u (5u/µl) 500u (5u/µl) 352202417 352302417

Hot Start Taq DNA Polymerase is designed for hot-start PCR, a technique that enhances the specificity, sensitivity and yield of DNA amplification. In addition, the enzyme provides the convenience of reaction set-up at room temperature. Hot Start Taq DNA Polymerase is a recombinant Taq DNA polymerase which has been chemically modified by the addition of heat-labile blocking groups to its amino acid residues. Applications: High yield amplification of targets up to 3 kb., Hot-start PCR, RT-PCR, Highly specific amplification of complex genomic and cDNA templates, Amplification of low copy DNA targets, Multiplex PCR, Generation of PCR products for TA cloning and Real-time PCR. SmarTaq DNA Polymerase (Recombinant) TA8108C TA8109C TA8110C 100u (5 u/µl) 500u (5 u/µl) 5000u (5 u/µl) 616201414 616301414 616601414

3

Supplied with: 10XPCR Buffer 100mM Tris-HCl (pH 8.8 at 25°C), 500mM KCl , 10X PCR Buffer (with 750mM Tris-HCl (pH 8.8 at 25°C), 200mM (NH4)2SO4, 0.1% Tween 20 and 50mM MgCl2.

AMS (Ammonium Sulfate) 10X PCR Buffer is not included. It can be provided free of charge upon request with any purchase.

Taq DNA Polymerase (Recombinant) TA7505C TA7506C TA7507C 50mM MgCl2. 23 100U(5 u/µl) 500U(5 u/µl) 5000U(5 u/µl) 616201417 616301417 616601417

Supplied with: 10XPCR Buffer 100mM Tris-HCl (pH 8.8 at 25°C), 500mM KCl and

The Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. Source : E.coli cells with a cloned pol gene from Thermus aquaticus. Taq DNA Polymerase (Recombinant) EP0401 100u (5u/µl) 500u (5u/µl) 316202418 316302418

3

EP0402

The Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. Source: E.coli cells with a cloned gene from Thermus aquaticus. Taq DNA Polymerase (native, With out BSA) EP0281 EP0282 100u (5u/µl) 500u (5u/µl) 316202418 316302418

The Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. Source: Thermus aquaticus YT1 cells. Pfu DNA Polymerase (Recombinant) EP0501 EP0502 100u, 2.5u/µl 500u, 2.5u/µl 616201459 616301459

The Pfu DNA Polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeum Pyrococcus furiosus. The Pfu DNA Polymerase also exhibits 3'=>5' exonuclease (proofreading) activity, that enables the polymerase to correct nucleotide incorporation errors. It has no 5'=>3' exonuclease activity. Source: Pfu DNA Polymerase (recombinant) - E.coli cells with a cloned pol gene from Pyrococcus furiosus. High Fidelity PCR Enzyme Mix K0191 K0192 100u (5u/µl) 500u (5u/µl) 354202288 354302288

High Fidelity PCR Enzyme Mix is a unique blend of Taq DNA Polymerase and a 24

thermostable DNA Polymerase with proofreading activity. The Mix can amplify long templates (up to 20 kb from viral DNA and up to 10 kb from genomic DNA), which is often difficult to achieve in PCR using a single enzyme. Long PCR Enzyme Mix K0181 K0182 100u (5u/µl) 500u (5u/µl) 316302288 316402288

3

Long PCR Enzyme Mix is a unique blend of Taq DNA Polymerase and a thermostable DNA Polymerase with proofreading activity. Long PCR Enzyme Mix is optimized for amplification of very long (up to 47 kb from viral DNA and up to 21 kb from genomic DNA) products with three times higher accuracy than with Taq DNA Polymerase. PCR products generated using Long PCR Enzyme Mix have mainly single dA overhangs at the 3'-end; cloning of PCR products by the T/A method is recommended. PCR SET System PR7815C 616301396 This set provides qualified reagents for amplification of different DNA templates and consisted: SmarTaq (5u/ul), 10X PCR buffer (KCl base), 10X AMS buffer (Ammonium Sulfate base), 50 mM MgCl2 and 10 mM dNTP. These reagents are sufficient for 100 reactions of 100 ul each. 2X PCR Master Mix PCR master kit, 200 Tests PCR Master Kit, 40 Tests 616301394 616201394

2X PCR Master Mix is a premixed 2X concentrated solution of Taq DNA Polymerase, reaction buffer, MgCl2 and dNTPs. 2X PCR Master Mix contains all components for PCR, except DNA template and primers. Composition of Master Mix: 0.04u/µl Taq DNA Polymrease (recombinant), reaction buffer, 3mM MgCl2, 0.4mM of each dNTP(dATP, dCTP, dGTP, dTTP).

25

PyroStartTM Fast PCR Master Mix (2X) K0211 2x1.25ml for 250 reactions of 20µl 316102354 PyroStartTM Fast PCR Master Mix (2X) is designed for fast, specific, sensitive and reproducible PCR. It is a proprietary formulation of a chemically-modified hot start Taq DNA Polymerase, specifically designed for fast reactivation. PyroStartTM Fast PCR Master

3

Mix (2X) has been optimized to reduce PCR time to just 25 minutes. PCR Check System PR7811C For 20 reactions 616201393 Provided separated reagents: 10X PCR buffer, 50 mM mgCl2, 10 mM dNTP, Taq DNA polymerase 5u/ul), DNA template and related Primers. This Kit has been successfully tested in several PCR reactions with varying annealing temperature from 40-65°C and annealing time duration from 10-60 seconds. Reaction conditions (concentration of each components, temperature or incubation time may be vary and evaluated by user. When you have any problem with your PCR system or you want to check your new reagents, you can use this Kit and evaluate your reaction. This Kit use as an educational Kit as well. Long PCR Control Set K0201 for 25 reactions 316502288 The Long PCR Control set is designed for optimization of long PCR cycling conditions and is used for quality control of Long PCR Enzyme Mix. The Set contains lambda DNA, one direct and two reverse primers designed for generation of 20 kb and 30 kb PCR fragments using Long PCR Enzyme Mix.

Nucleotides, molecular biology grade: dNTP Mixes:

dNTP Mixes are aqueous solutions at pH 7.0 containing dATP, dCTP, dGTP and dTTP, each at a final concentration of either 10mM. 26

dNTP Mix, 10mM each DN7603C DN7604C dNTP/dUTP Mix R0251 1.0ml 316002101 0.1ml 0.5ml 616201101 616301101

3

dNTP/dUTP Mix is an aqueous solution containing dATP, dCTP, dGTP, each at a final concentration of 2mM and dUTP of 4mM. dNTP Set DN0181C (4x0.25ml of 100mM solutions) 616001100 dNTP Set consits of 100mM aqueous solutions at pH 7.0 of each of dATP, dCTP, dGTP and dTTP, each in a separate vial. dUTP R0133 to pH 7.0. (0.25ml of 100mM solution) 316002296 dUTP (2'-deoxyuridine 5'-triphosphate) is supplied as 100mM aqueous solution titrated

PCR buffers and reagents:

10X PCR Buffer CG7507 1ml 616001355 100mM Tris-HCl (pH 8.8 at 25°C), 500mM KCl. 10X Taq Buffer with KCl B38 5ml (5x1ml) 100mM Tris-HCl (pH 8.8 at 25°C), 500mM KCl, 0.8% Nonidet P40. 316202355

27

AMS 10X PCR Buffer with (NH4)2SO4 CG8108C 1ml 616001354 750mM Tris-HCl (pH 8.8 at 25°C), 200mM (NH4)2SO4, 0.1% Tween 20. 50mM MgCl2

3

TP7506C Water, nuclease-free DW8505 DW8520 R0581 R0582

0.5ml

616001390

5ml 20ml 5x1ml 30ml

400101851 400201851 316102288 316202288

Water, nuclease-free, is 0.22µm membrane-filtered molecular biology grade water suitable for use in all molecular biology applications. Bovine Serum Albumin B14 5mg, 20mg/ml 316002039 Bovine Serum Albumin (BSA) is suitable for stabilization of enzymes during storage and in in vitro reactions where the absence of contaminating nucleases is critical. DEPC MR7942 5ml 500101736

DEPC-treated Water, molecular biology grade MR8243C MR8244C 5ml 50ml 516101092 516201092

The DEPC-treated Water is deionized, diethylpyrocarbonate (DEPC) treated water. It is recommended to be used in any application where RNA is involved.

28

DTT, molecular biology grade R0861 R0862 5.0g 25.0g 216102304 216202304

DTT (dithiothreitol; Cleland's reagent) is used to quantitatively reduce disulfide bonds and maintain monothiols in a reduced state. At low concentrations, DTT stabilizes enzymes and other proteins which possess free sulfhydryl groups and has been shown to restore activity lost by oxidation of these groups in vitro. At a final 0.1M concentration DTT is also widely used for disruption of protein disulfide bonds in SDS-polyacrylamide gel electrophoresis.

3

First strand cDNA synthesis: M-MuLV Reverse Transcriptase

RevertAidTM M-MuLV Reverse Transcriptase EP0441 EP0442 10,000u (200u/µl) 5x10,000u (200u/µl) 316702412 316802412

The RevertAidTM M-MuLV Reverse Transcriptase (RT) is a genetically modified MMuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme posses an RNA-dependent and DNA-dependent polymerase activity and a ribonuclease H activity specific to RNA in RNA-DNA hybrids. Source: E.coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus reverse transcriptase.

RevertAidTM First Strand cDNA Synthesis Kits

RevertAidTM First Strand cDNA Synthesis Kit K1621 K1622 templates. 29 for 10 reactions for 100 reactions 316102255 316202255

The Kits are complete systems for efficient first strand cDNA synthesis from RNA

Both Kits use genetically engineered versions of the Moloney Murine Leukemia Virus (M-MuLV) reverse transcriptase. RevertAidTM M-MuLV Reverse Transcriptase has low RNase H activity, while RevertAidTM H Minus M-MuLV Reverse Transcriptase has a point mutation that completely eliminates RNase H activity. Either Kit is suitable for the synthesis of full-length cDNA as long as 13 kb, but greater yields of full-length cDNA from

3

longer templates are obtained with RevertAidTM H Minus M-MuLV Reverse Transcriptase. This enzyme gives better results because it does not degrade RNA in RNA-DNA hybrids during first strand cDNA synthesis and maintains activity over a wider temperature range. RevertAidTM H Minus M-MuLV Reverse Transcriptase RiboLockTM Ribonuclease Inhibitor 5X Reaction Buffer 10mM dNTP Mix Oligo(dT)18 Primer Random Hexamer Primer Control RNA Control Primer DEPC-treated Water RiboLockTM Ribonuclease Inhibitor EO0381 EO0382 2500u (40u/µl) 4x2500u (40u/µl) 316502967 316702967

RiboLockTM Ribonuclease Inhibitor inhibits the activity of RNases A, B and C by binding them in a noncompetitive mode at a ratio 1:1. It does not inhibit RNases 1, T1, T2, H, U1, U2, CL3 or other enzymes. Source: E.coli cells carrying a cloned gene encoding mammalian ribonuclease inhibitor. It protects RNA from degradation over temperature range of 25-55°C; some activity is still measured at 60°C.

30

Primer synthesis & Primers: PR7708C PR7709C OD/A260 = 5 ­ 7, 0.05µm OD/A260 = 15 ­ 20, 0.2µm 600101454 600201454

The Oligo synthesis is based on chemical synthesis of nucleotides in a 3' to 5' direction. This process is done by automatic machines and each step is controled by software. The purification is based on Reverse Phase Liquid (RP) chromatography in which only complete sequences are attached to C18 support. As a result, just minimum oligonucleotides (n-1 mer) will be remained in our final fraction. Oligodt 18 PR8115C ends. The primer is supplied as an aqueous solution and can be used for first strand cDNA synthesis. Primer Set PR8011C (Random 10mer ) Set 100 bas 0.05µm This primer is a mixture of random 10 nucleotides with 5'- and 3'-hydroxyl ends and it is qualified for random amplification Random Hexamer Primer SO142 hydroxyl ends. The primer is supplied as an aqueous solution and can be used for first strand cDNA synthesis. 120µl (24µg), 100µM (0.2µg/µl) 316002252 This primer is a mixture of single-stranded random hexanucleotides with 5'- and 3'612301406 30µg 616301211

3

This primer is a synthetic single-stranded 18-mer oligonucleotide with 5'- and 3'-hydroxyl

31

Sequencing Services: Single-Read Sequencing

Short run: Up to 400 bases Medium run: Up to 900 bases 600101455 600201455 659101455 400001454

3

PCR- Purification Primer Design

DNA Electrophoresis

6X Loading Buffer MR7723C Ethidium Bromide MR7721C Ethidium Bromide MR7729 Agarose MR7720 Agarose MR7730C AgaroseTopVision LM GQ R0801 (Low Melting) 25g 216202353 50g 416201352 10g 416101352 250mg 516001426 1ml 316002426 1ml 616101008

32

TBE Buffer, 5X MR7725C 50 ml 616201419

DNA Ladders & Markers:

GeneRulerTM 50bp, 50-1000 bp SM0371 50µg 316002139

3

GeneRulerTM 50bp, 50-1000 bp, Ready-to-use SM0373 50µg 316202139

GeneRulerTM 1kb, 250-10000bp SM0311 250 (5x50) µg 316002138

GeneRulerTM 1kb, 250-10000bp , Ready-to-use SM0313 250(5x50) µg 316302138

GeneRulerTM 100bp Plus, 100-3000bp SM0321 50µg 316002137

GeneRulerTM 100bp Plus, 100-3000bp, Ready-to-use SM0323 50µg 316202137

GeneRulerTM 100bp, 80-1000bp SM0241 50µg 316002136

GeneRulerTM100bp , 80-1000bp, Ready-to-use SM0243 50µg 316202136

33

GeneRulerTM Mix, 100-10000bp SM0331 250(5x50) µg 316102140

GeneRulerTM Mix, 100-10000bp, Ready-to-use SM0333 250 (5x50) 50µg 316302140

3

Lambda DNA/EcoRI+HindIII Marker 3, 125-21226bp SM0191 250 (5x50) µg 316002169

PhiX174 DNA/ BsuRI (HaeIII) Marker 9, 72-1353bp SM0251 50µg 316002001

PhiX174 DNA/ BsuRI (HaeIII) Marker 9, Ready-to-use 72-1353bp SM0253 50µg 31620200

PCR Mineral oil (Molecular grade) MI7728 5ml 416001395

PCR Mineral oil (Molecular grade) MI8029 20ml 416101395

PCR Mineral oil (Molecular grade) MI8130 50ml 416201395

PCR Mineral oil (Molecular grade) MI8231 500ml 416301395

34

Molecular Cloning

4

Cloning and Expression:

Kits for cloning

GeneJETTM PCR Cloning Kit K1221 for 20 reactions for 40 reactions

TM

316102990 316202990

4

K1222 The GeneJET

PCR Cloning Kit is an advanced positive selection system for the highest

efficiency cloning of PCR products generated with any DNA polymerase. The kit offers cloning efficiencies of up to 100% positive clones, eliminating the need for tedious colony screening. The kit is specifically designed to ligate PCR products into a positive selection vector in just 5 minutes. Purification of PCR products is not required. Fragment with any end structure can be used: blunt-end, dA tailed, sticky-end, with or with out 5' ­ phosphates. Components of the Kit pJET1/blunt T4 DNA Ligase 2X Reaction Buffer Blunting Enzyme pJET1 Forward Sequencing Primer pJET1 Reverse Sequencing Primer Control PCR Product Water, nuclease-free InsTAcloneTM PCR Cloning Kit K1213 K1214 The InsTAclone

TM

for 10 reactions for 30 reactions

316102159 316202159

PCR Cloning Kit is a TA system for direct one-step cloning of PCR

products with 3'-dA overhangs. Overhangs of this type are generated by Taq DNA 36

polymerase and other thermostable DNA polymerases which lack proofreading activity. Components of the Kit Vector pTZ57R/T T4 DNA Ligase 5X Ligation Buffer Control PCR Fragment Control DNA (pTZ57R with insert) Vector pTZ57R DNA (without insert) Water, nuclease-free TransformAidTM Bacterial Transformation System: C-Medium T-Solution (A) T-Solution (B) IPTG MR7722 MR7739 1g 5g 616001317 616101317

4

IPTG (isopropyl-beta-D-thiogalactopyranoside) induces activity of beta-galactosidase, an enzyme that promotes lactose utilization, by binding and inhibiting the lac repressor. IPTG is used to induce lacZ gene expression in cloning experiments. X-Gal R0401 R0402 R0404 X-Gal 0.5g 2.0g 1.0g (5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) is a 316002293 316202293 316102293 noninducing

chromogenic substrate for beta-galactosidase, which hydrolyzes X-Gal forming an intense blue precipitate. X-Gal is most frequently used in conjunction with IPTG in blue/white colony screening to detect recombinants (white) from non-recombinants (blue). 37

X-Gal Solution, ready-to-use R0941 10ml, 20mg/ml 216102337 X-Gal (5-brom-4-chloro-3-indolyl-beta-D-galactopyranoside), a ready-to-use solution, is specially formulated for direct use in blue/white colony screening assays. Plasmids: pBR322 25 µg 25 µg 25 µg 25 µg 25 µg 25 µg 25 µg 25 µg 25 µg 25 µg 25 µg 25 µg 25 µg 25 µg 25 µg PL7601C PL7602C PL7604C PL7603C PL7605C PL7606C PL7607C PL7608C PL7609C PL7611C PL7612C PL7613C PL7614C PL7615C PL7616C 616001444 616001398 616001399 616001445 616001400 616001402 616001446 616001447 616001401 616001448 616001449 616001450 616001451 616001244 616001245

4

pGEM-3Z pGEM-4Z pGEM-3Zf(-) pGEM-5Zf(+) pGEM-5Zf(-) pGEM-7Zf(+) pGEM-7Zf(-) pGEM-9Zf(-) pSP 70 pSP 71 pSP 72 pSP 73 pUC18 pUC19 Bacterial Strains E.coli BL-21 (DE3) E.coli DH5a E.coli HB101 E.coli TGI E.coli XL1-Blue

One Vial One Vial One Vial One Vial One Vial

BA7601C BA7607C BA7602C BA7604C BA7605C

500101427 500101428 500101429 500101430 500101431

38

Cloning vectors & phage DNA

Lambda DNA SD0011 500µg, 0.3µg/µl 316002163 Lambda DNA is commonly used as a substrate in restriction enzyme activity assays and for preparation of DNA molecular weight standards. The phage is isolated from a heatinducible lysogenic E.coli W3110 (cI857 Sam7) strain. The DNA is isolated from purified phage by standard purification procedure. pUC18 DNA PL7615C 25µg, 0.5µg/µl 616001244 pUC18 DNA is a commonly used plasmid cloning vector in E.coli. The molecule is a double-stranded circle, 2686 base pairs in length. pUC19 DNA PL7616C 25µg, 0.5µg/µl 616001245 pUC19 DNA is a commonly used plasmid cloning vector in E.coli. The molecule is a double-stranded circle, 2686 base pairs in length. pUC57 DNA SD0171 50µg, 0.5µg/µl 316002249 pUC57 DNA is a commonly used plasmid cloning vector in E.coli. The molecule is a double-stranded circle, 2710 base pairs in length. pBR322 DNA PL7601C 25µg, 0.5µg/µl 616001444 pBR322 DNA is a commonly used plasmid cloning vector in E.coli. The molecule is a double-stranded circle, 4361 base pairs in length.

4

39

pTZ19R DNA SD0141 50µg, 0.5µg/µl 316002242 pTZ19R DNA is a small phagemid designed for DNA cloning, dideoxy DNA sequencing, in vitro mutagenesis and in vitro transcription in one system. The molecule is a doublestranded circle, 2862 base pairs in length.

Microbiological media & additives:

4

Ampicillin E.coli FastMediaTM:

FastMediaTM LB Liquid Amp M0011 FastMediaTM LB Agar Amp M0021 20 pouches 116202342 20 pouches 116202346

FastMediaTM LB Agar Amp IPTG/X-Gal M0031 20 pouches 116202343 Ampicillin FastMediaTM Bacterial Media are microwavable ready-to-use solid or liquid media, supplied with a selective antibiotic, and designed for the growth or selection of ampicillin resistant colonies of E.coli as well as detection of blue-white colonies. FastMediaTM Bacterial Media are packaged in individual pouches each containing the necessary ingredients for the preparation of 200ml of selective sterile culture medium within 10min or 8 to 10 selective plates within 20min . X-Gluc R0851 R0852 0.1g 0.5g 316202294 316302294

X-Gluc (5-bromo-4-chloro-3-indolyl-beta-D-glucuronic acid, cyclohexylammonium salt) is a substrate for beta-glucoronidase (GUS) encoded by the gusA gene. X-Gluc is suitable for detection of GUS expression in cells and tissue. 40

DMSO CR8014 10ml 316103470

DTT, molecular biology grade R0861 R0862 5.0g 25.0g 216102304 216202304

DTT (dithiothreitol; Cleland's reagent) is used to quantitatively reduce disulfide bonds and maintain monothiols in a reduced state. At low concentrations, DTT stabilizes enzymes and other proteins which possess free sulfhydryl groups and has been shown to restore activity lost by oxidation of these groups in vitro. At a final 0.1M concentration DTT is also widely used for disruption of protein disulfide bonds in SDS-polyacrylamide gel electrophoresis.

4

DNA Electrophoresis DNA Ladders & Markers:

GeneRulerTM 50bp, 50-1000 bp SM0371 50µg 316002139

GeneRulerTM 50bp, 50-1000 bp, Ready-to-use SM0373 50µg 316202139

GeneRulerTM 1kb, 250-10000bp SM0311 250 (5x50) µg 316002138

GeneRulerTM 1kb, 250-10000bp , Ready-to-use SM0313 250(5x50) µg 316302138

GeneRulerTM 100bp Plus, 100-3000bp SM0321 50µg 316002137 41

GeneRulerTM 100bp Plus, 100-3000bp, Ready-to-use SM0323 50µg 316202137

GeneRulerTM 100bp, 80-1000bp SM0241 50µg 316002136

GeneRulerTM100bp , 80-1000bp, Ready-to-use

4

SM0243

50µg

316202136

GeneRulerTM Mix, 100-10000bp SM0331 250(5x50) µg 316102140

GeneRulerTM Mix, 100-10000bp, Ready-to-use SM0333 50 (5x50) 50µg 316302140

Lambda DNA/EcoRI+HindIII Marker 3, 125-21226bp SM0191 250 (5x50) µg 316002169

PhiX174 DNA/ BsuRI (HaeIII) Marker 9, 72-1353bp SM0251 50µg 316002001

PhiX174 DNA/ BsuRI (HaeIII) Marker 9, ready-to-use, 72-1353bp SM0253 6X Loading Buffer MR7723C Ethidium Bromide MR7721 42 1ml 316002426 1ml 616101008 50µg 316202001

Ethidium Bromide MR7729C Agarose MR7720 Agarose MR7730C AgaroseTopVision LM GQ R0801 (Low Melting) TBE Buffer, 5X MR7725C 50 ml 616201419 25g 216202353 50g 416201352 10g 416101352 250mg 516001426

4

Sequencing Services: Single-Read Sequencing

Short run: Up to 400 bases Medium run: Up to 900 bases PCR- Purification Primer Design 600101455 600201455 659101455 400001454

43

5 Nucleic Acid Purification

DNA / RNA Extraction:

Plasmid Extraction Kit PS8405C for 50 prep 416201918 In this procedure, Inorganic Salts crystals utterly simplified separation of phases. Then Plasmid DNA captured by sorbent and after twice washing pure plasmid DNA eluted by water. Components of the Kit Resuspension Solution Lysis Solution K acetate Solution Co-Precipitation Solution (Inorganic Salt) Binding Buffer Elution Buffer GeneJETTM Plasmid Miniprep Kit K0501 K0502 K0503 Components of the Kit Resuspension Solution Lysis Solution Neutralization Solution Wash Solution (concentrated) RNase A Elution Buffer Spin Columns Collection Tubes 45 for 25 preps for 50 preps for 250 preps 316202989 316302989 316402989

5

Plasmid Miniprep Kit provides a simple, rapid and cost-effective method for the isolation of high quality plasmid DNA from recombinant E.coli cultures. DNA Extraction Kit from agarose gels K0513 for 100 preps 216202303 (up to 5 µg of DNA per prep) The Kit provides a simple and efficient method for DNA extraction from agarose gels. It is also an ideal tool to desalt DNA solutions and remove residual organic solvents, unincorporated nucleotides, primers, primer-dimers and enzymes from labeling reactions

5

or PCR. The purified DNA is suitable for any molecular biology procedure: restriction digestion, cloning, sequencing, etc. Components of the Kit: Silica Powder Suspension Binding Solution Concentrated Washing Buffer TBE Conversion Buffer DNA Extraction Kit (DNPTM) DN8115C 50 Tests 616201303 Guanidine salt base and a complete kit for DNA extraction from: 100µl of whole blood (fresh, frozen or dried spots), 100µl Sera ( for viral DNA extraction), Homogenized sputum (for detection of M. tuberculosis), CSF, Cell Culture ( 0.4-0.6x106 of cultured cells) , 25-30mg of mammalian tissue , Bacterial Cultures (10-20mg of bacteria for PCR application), Buccal or Vaginal Swab, formalin-fixed paraffin-embedded tissue or section. DNA obtained by this method can be used for all molecular biology procedures (PCR, restriction digestion, cloning, Southern blot, DNA sequencing, etc.) Typical DNA yield is in range of 10-20 µg from 100µl fresh whole blood.

46

Components of the Kit: Protease and Protease buffer Lysis Solution Precipitation Solution Wash Solution Solvent DNA Extraction Solution (DNG-plusTM) DN8117C DN8118C 20 ml 100ml 616101435 516201435

Guanidine base and a single solution for DNA extraction from: 100µl of whole blood (fresh or frozen), Homogenized sputum (for detection of M. tuberculosis), Buccal or Vaginal swab. Genomic DNA Purification Kit K0512 The prep uses: 200µl of whole blood 25-30mg of mammalian tissue 50-100mg of plant tissue 0.4-0.6x106 of cultured cells or 10-20mg of bacteria The Genomic DNA Purification Kit is based on a very simple and rapid procedure of selective DNA precipitation (using detergents) from crude lysates without a phenol extraction step. High quality RNA-free DNA can be prepared from whole blood, serum, cell cultures, bacteria, tissues and epithelial samples in 20-25min. The yield of DNA (2-10µg from 0.2ml of blood) is quantitative and reproducible, and the DNA is of sufficient purity for direct use in any application, e.g. PCR, restriction digestion, cloning, DNA sequencing, Southern blot analysis, etc. for 100 preps 116202350

5

47

Components of the Kit: Lysis Solution Precipitation Solution NaCl Solution. Phenol Equilibrated MR7841C Phenol Tris saturated Phenol extracted with Tris buffer; stabilized with Hydroxyquinoline; pH (20°C): 8.0-8.5. 20ml 516201403

5

RNXTM-plus solution RN7713C 25ml 516201410 RNX solution provided a reliable and advanced method for total RNA isolation from tissue, blood, bacterial cell, homogenized sample, serum or plasma (RNA viruses). Through the action of Guanidine salt in RNA extraction, simultaneously DNA and protein are precipitated in phenol phase. The obtained RNA has high quality and contains all types of RNA. DEPC MR7942 5ml 500101736

DEPC-treated Water, molecular biology grade MR8243C MR8244C 5ml 50ml 516101092 516201092

The DEPC-treated Water is deionized, diethylpyrocarbonate (DEPC) treated water. It is recommended to be used in any application where RNA is involved. DTT, molecular biology grade R0861 R0862 48 5.0g 25.0g 216102304 216202304

DTT (dithiothreitol; Cleland's reagent) is used to quantitatively reduce disulfide bonds and maintain monothiols in a reduced state. At low concentrations, DTT stabilizes enzymes and other proteins which possess free sulfhydryl groups and has been shown to restore activity lost by oxidation of these groups in vitro. At a final 0.1M concentration DTT is also widely used for disruption of protein disulfide bonds in SDS-polyacrylamide gel electrophoresis. Glycogen, molecular biology grade R0561 Glycogen, RNA grade (has been specifically designed for RNA precipitation). R0551 2x0.1ml Glycogen is a highly purified polysaccharide derived from oysters. It is an inert carrier which significantly increases the recovery of nucleic acids by alcohol precipitation. Proteinase K EO0491 EO0492 1ml (~600u/ml, 20mg/ml) 5x1ml (~600u/ml, 20mg/ml) 316102407 316202407 2x0.25ml 316002141

5

Proteinase K is an endolytic protease that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic or hydrophobic amino acids. Proteinase K is classified as a serine protease. The smallest peptide to be hydrolyzed is a tetrapeptide. Source: Tritirachium album. Agarase EO0461 100u (0.5u/µl) 316202874 Agarase specifically digests the agarose polysaccharide core made up of repeating 1,3linked beta-D-galactopyranose and 1,4-linked 3,6-anhydro-alfa-L-galactopyranose into neoagaro-oligosaccharides. Source: E.coli cells carrying a plasmid with a cloned gene encoding beta-agarase from Pseudomonas atlantica.

49

Lysozyme MR7735C 500mg 516001471

Sodium Dodecyl Solfate ( SDS ) MR7727 MR8247 Sarcosyl MR7737 50g 416201413 50g 250g 416201380 416301380

5

Titriplex III (EDTA) MR7732 50g 409201420

0.5M EDTA, pH 8.0, molecular biology grade R1021 5x1ml 216102298

Sodium Acetate (3M pH 5.2) MR8062C Tris Base MR7734 Tris Base MR7738 500g 416301423 50g 416201423 5ml 516101783

50

In Vitro Transcription

6

Kits for transcription

T7 Transcription Kit K0411 K0412 for 10 reactions (of 50µl) for 30 reactions (of 50µl) 316102276 316202276

T7 Transcription Kit is a convenient in vitro transcription system using T7 RNA Polymerase and a vector bearing a T7 promoter sequence (pTZ19R). The Kit is designed for the preparation of microgram quantities of unlabeled or labeled single-stranded RNA transcripts from a cloned DNA insert. Components of the Kit:

6

T7 RNA Polymerase 5X Transcription Buffer RiboLockTM Ribonuclease Inhibitor ATP Solution CTP Solution GTP Solution UTP Solution Vector pTZ19R DNA Control Template DEPC-treated Water

Enzymes for transcription

Bacteriophage SP6, T3 and T7 RNA polymerases are DNA-dependent RNA polymerases with strict specificity for their respective double-stranded promoters. They catalyze the 5'=>3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from their promoters. Source: E.coli cells with a cloned gene encoding the corresponding RNA polymerase. 52

SP6 RNA Polymerase EP0131 T3 RNA Polymerase EP0101 EP0102 T7 RNA Polymerase EP0111 EP0112 5000u (20u/µl) 5x5000u (20u/µl) 316602947 316702947 2000u (20u/µl) 10,000u (20u/µl) 316502274 316702274 2000u (20u/µl) 316502270

RiboLockTM Ribonuclease Inhibitor EO0381 EO0382 2500u (40u/µl) 4x2500u (40u/µl) 316502967 316702967

6

RiboLockTM Ribonuclease Inhibitor inhibits the activity of RNases A, B and C by binding them in a noncompetitive mode at a ratio 1:1. It does not inhibit RNases 1, T1, T2, H, U1, U2, CL3 or other enzymes. Source: E.coli cells carrying a cloned gene encoding mammalian ribonuclease inhibitor. It protects RNA from degradation over temperature range of 25-55°C, some activity is still measured at 60°C.

53

7 Protein / DNA Ladders & Markers

Protein

Protein Ladders & Markers:

PageRulerTM Prestained Protein Ladder Plus SM1811 2x250µl, 10-250kDa 316102219

PageRulerTM Prestained Protein Ladder SM0671 2x250µl, 10-170KDa 316002219

PageRulerTM Unstained Protein Ladder SM0661 2x250µl, 10-200kDa 316002220

Prestained Protein Molecular Weight Marker SM0441 2x250µl, 20-120kDa 316002234

7

Unstained Protein Molecular Weight Marker SM0431 2x1000µl, 14.4-116kDa 316102235

Reagents for Protein Electrophoresis:

PageBlueTM Protein Staining Solution R0571 PageBlue

TM

1 liter (for up to 150 mini-gels)

116402351

Protein Staining Solution is a ready-to-use solution for a highly sensitive

staining of proteins separated in polyacrylamide gels. PageBlueTM contains Coomassie Brilliant Blue G-250 dye. Detects as little as 5ng of protein. Protein Standard Solution R0882 10x1m 200µg/ml 316102236 200µg BSA/ml in 0.15M NaCl containing 0.05% sodium azide as preservative. Protein 55

Standard Solution is used for colorimetric determination of total protein concentration by Lowry method. Loading Buffer Pack R0891 5X Loading Buffer 20ml 20X Reducing Agent gel electrophoresis (SDS-PAGE). Acrylamide MR8061 MR8264 Bis Acrylamide MR8062 Boric Acid MR7731 500g 416301468 10g 533101365 100g 500g 416201356 416301356 4x1.5ml 316002174

Loading Buffer Pack is used for preparation of protein samples for an SDS-polyacrylamide

7

Sodium Dodecyl Solfate ( SDS ) MR7727 MR8247 50g 250g 416201380 416301380

DNA Electrophoresis

Agarose MR7720 Agarose MR7730C 56 50g 416201352 10g 416101352

AgaroseTopVision LM GQ R0801 (Low Melting) Ethidium Bromide MR7721 Ethidium Bromide MR7729C 250mg 516001426 1ml 316002426 25g 216202353

DNA Ladders & Markers:

GeneRulerTM 50bp, 50-1000 bp SM0371 50µg 316002139

GeneRulerTM 50bp, 50-1000 bp, Ready-to-use SM0373 50µg 316202139

7

GeneRulerTM 1kb, 250-10000bp SM0311 250 (5x50) µg 316002138

GeneRulerTM 1kb, 250-10000bp , Ready-to-use SM0313 250(5x50) µg 316302138

GeneRulerTM 100bp Plus, 100-3000bp SM0321 50µg 316002137

GeneRulerTM 100bp Plus, 100-3000bp, Ready-to-use SM0323 50µg 316202137

57

GeneRulerTM 100bp, 80-1000bp SM0241 50µg 316002136

GeneRulerTM100bp , 80-1000bp, Ready-to-use SM0243 50µg 316202136

GeneRulerTM Mix, 100-10000bp SM0331 250(5x50) µg 316102140

GeneRulerTM Mix, 100-10000bp, Ready-to-use SM0333 250 (5x50) 50µg 316302140

Lambda DNA/EcoRI+HindIII Marker 3, 125-21226bp

7

SM0191

250 (5x50) µg

316002169

PhiX174 DNA/ BsuRI (HaeIII) Marker 9, 72-1353bp SM0251 50µg 316002001

PhiX174 DNA/ BsuRI (HaeIII) Marker 9, ready-to-use, 72-1353bp SM0253 6X Loading Buffer MR7723C TBE Buffer, 5X MR7725C 50 ml 616201419 1ml 616101008 50µg 316202001

58

Nucleotides

8

Nucleotides, molecular biology grade:

dNTP Mixes:

dNTP Mixes are aqueous solutions at pH 7.0 containing dATP, dCTP, dGTP and dTTP, each at a final concentration of either 10mM. dNTP Mix, 10mM each DN7603C DN7604C dNTP/dUTP Mix R0251 1.0ml 316002101 dNTP/dUTP Mix is an aqueous solution containing dATP, dCTP, dGTP, each at a final 0.1ml 0.5ml 616201101 616301101

8

concentration of 2mM and dUTP of 4mM. dNTP Set DN0181C (4x0.25ml of 100mM solutions) 616001100 dNTP Set consits of 100mM aqueous solutions at pH 7.0 of each of dATP, dCTP, dGTP and dTTP, each in a separate vial. dUTP R0133 to pH 7.0. (0.25ml of 100mM solution) 316002296 dUTP (2'-deoxyuridine 5'-triphosphate) is supplied as 100mM aqueous solution titrated

60

PCR Detection Kits

9

PCR Detection Kits:

Hepatitis B Virus PCR Detection Kit PR7813C For 50 Tests 616301383 This kit is destined for qualitative detection of HBV DNA in serum and plasma of Human blood, by the method of Polymerase Chain Reaction. This Kit contains all needed PCR reagents: ready to use 1X PCR mix and it consisted: 10X buffer, MgCl2, dNTP, loading buffer, specific forward and reverse primers and PCR reaction enhancers at final concentration, Taq DNA polymerase, and DNA template as PCR positive control High conserved region of Surface Antigen considered as target and 353 bp fragment in compare with positive control indicated as a positive test. For DNA extraction we recommended CinnaGen DNA Extraction Kit (DNPTM Kit), 616201303. STRPTM Hepatitis C Virus Detection Kit PR7832C For 50 Tests (single tube RT-PCR) 616301384

This kit is destined for qualitative detection of HCV RNA in the serum and plasma of

9

Human blood by the method of Single tube RT reaction, followed by nested PCR. This Kit contains all needed PCR reagents: ready to use 1x PCR mix 1 and 2 and it consisted: 10x buffer, MgCl2, dNTP, loading buffer, specific outer and inner primers and PCR reaction enhancers at final concentration, Taq DNA polymerase, Reverse transcriptase with RNasin, DNA template as PCR positive control, DEPC- treated water for RNA dissolving and high quality solution for low copy RNA Extraction (RNX-Plus). CinnaGen HCV-PCR detection Kit allows you to use RNA in PCR tube directly and ignore separated cDNA synthesis protocol. High conserved region of HCV genome, or NCR considered as target and 216 bp fragments in compare with positive control indicated as a positive test. Mycobacterium tuberculosis PCR Detection Kit PR7935C For 50 Tests 616301391 This kit is designed for qualitative detection of Mycobacterium tuberculosis (MTB) DNA 62

in the Human sample by the method of Polymerase Chain Reaction. This Kit contains all needed PCR reagents: ready to use 1X PCR mix and it consisted: 10X buffer, MgCl2, dNTP, loading buffer, specific forward and reverse primers at final concentration, Taq DNA polymerase, TB lysis solution and DNA template as PCR positive control For DNA extraction from liquefied, decontaminated Sputum, or Urine samples using CinnaGen DNGTM-Plus (616101435). For DNA extraction from biopsy paraffin-embedded tissue use CinnaGen DNPTMKit (616201303). For DNA Extraction from culture media, use TB Lysis Solution (supplied). IS6110 considered as target and 163 bp fragment in compare with positive control indicated as a positive test. Cytomegalovirus PCR Detection Kit PR7836C For 50 Tests 616301373 CinnaGen CMV PCR Detection Kit is able to detect the presence of human CMV DNA in clinical specimens by the method of Polymerase Chain Reactions. This Kit contains all needed PCR reagents: ready to use 1X PCR mix and it consisted: 10X buffer, MgCl2, dNTP, loading buffer, specific forward and reverse primers at final concentration, Taq DNA polymerase, and DNA template as PCR positive control. For DNA extraction from Whole blood, urine sediment use CinnaGen DNGTM-Plus (616101435). For DNA extraction from tissue, use CinnaGen DNPTMKit ( 616201303). Early gene considered as target gene and presence of 222 bp fragment in compare with positive control indicated as a positive test. Herpes Simplex virus I&II Detection Kit PR8240C For 20 tests 616201385 This kit is destined for qualitative detection of Herpes simplex Virus I & II (HSV I & II) DNA in the human sample by the method of Polymerase Chain Reaction. CinnaGen HSV PCR Detection Kit may be used in clinical medicine to detect HSV I & II DNA. This Kit contains all needed PCR reagents: ready to use 1X PCR mix and it consists: 63

9

10X buffer, MgCl2, dNTP, loading buffer, specific forward and reverse primers at final concentration. Taq DNA polymerase, and DNA template as PCR positive control. DNA Polymerase Gene considered as target and 256 bp fragment in compare with positive control indicated as a positive test. CinnaGen DNGTM ( 616101435) is recommended for DNA extraction from CSF samples. Helicobacter pylori PCR Detection Kit PR7843C For 20 tests 616201382 This kit is destined for qualitative detection of Helicobacter pylori DNA in culture or human gastric biopsy by the method of Polymerase Chain Reaction.This Kit contains all needed PCR reagents: ready to use 1X PCR mix and it consisted: 10X buffer, MgCl2, dNTP, loading buffer, specific forward and reverse primers at final concentration, Taq DNA polymerase, and DNA template as PCR positive control Sample DNA can be extracted using CinnaGen DNPTM ( 616201303). Urease C considered as target gene and presence of 492 fragment in compare with positive control indicated as a positive test. STRPTM HIV Detection Kit PR8249C For 20 tests (single tube RT-PCR) 616101442

9

This kit is destined for qualitative detection of HIV RNA-1 in the serum and plasma of Human blood by the method of Single tube RT reaction, followed by nested PCR. This Kit contains all needed PCR reagents: ready to use 1x PCR mix 1 and 2 and it consisted: 10x buffer, MgCl2, dNTP, loading buffer, specific outer and inner primers and PCR reaction enhancers at final concentration, Taq DNA polymerase, Reverse transcriptase with RNasin, DNA template as PCR positive control, DEPC- treated water for RNA dissolving and high quality solution for low copy RNA Extraction (RNX-Plus). CinnaGen HIV1-PCR detection Kit allows you to use RNA in PCR tube directly and ignore separated cDNA synthesis protocol. Hpol gene considered as target and 174 bp fragment in compare with positive control indicated as a positive test.

64

Leishmania sp. PCR Determination and Detection Kit PR7937C For 50 Tests 616201440 This kit is designed for qualitative detection of Leishmania sp. kinetoplast DNA (kDNA) in the Human sample by the method of Polymerase Chain Reactions. Using this kit species of Leishmania Parasite could be determined by different PCR products size. CinnaGen Leishmania sp. PCR Determination and Detection Kit may be used in clinical medicine to detect Leishmania sp.kDNA. This Kit contains all needed PCR reagents: ready to use 1X PCR mix and it consisted: 10X buffer, MgCl2, dNTP, loading buffer, specific forward and reverse primers at final concentration, Taq DNA polymerase, and DNA template as PCR positive control For DNA Extraction from culture media DNGTM -plus (616101435). is recommended. DNA Extraction from wound and biopsy samples needs Protease treatment and could be extracted by CinnaGen DNPTM (616201303) Kit. In compare with PCR Positive control and 100 bp DNA ladder, the presence of 620 bp fragment indicate of L. major and 800 bp indicates of L. tropica. L.infantum also produces 800 bp fragment that should be determined according kind of sample and clinical history. Neisseria gonorrhoeae PCR Detection Kit PR7844C For 20 tests 616201392 This kit is destined for detection of Neisseria gonorrhea DNA in endocervical, urethral, urine sample or Bacterial culture by the method of Polymerase Chain Reaction. This Kit contains all needed PCR reagents: ready to use 1X PCR mix and it consisted: 10X buffer, MgCl2, dNTP, loading buffer, specific forward and reverse primers at final concentration, Taq DNA polymerase, and DNA template as PCR positive control Sample DNA can be extracted using CinnaGen DNGTM ( 616101435). Presence of 420 bp fragment in compare with positive control indicated as a positive test. Sex Determination PCR Kit PR8048C For 20 tests 616201441 65

9

Ready to use Sex determination PCR Kit is destined for sex detection before delivery in the first trimester or after delivery, forensic, or archeology by the method of polymerase chain reactions. Cinnagen sex determination PCR kit may be used in medicine to determine sex of the fetus. This kit simultaneously amplifies a X-specific and Y-specific DNA fragments, which are specific to the X-Y homologous Amelogenin gene. This kit consists of internal control (X-Chromosome.) This kit contains all needed PCR reagents: ready to use 1X PCR mix and it consisted: 10X buffer, MgCl2, dNTP, loading buffer, specific forward and reverse primers at final concentration, Taq DNA polymerase, and DNA template as PCR positive control. The presence of two band of 977 and 788bp from X and Y chromosome were indicative of male sex. On the other hand, the presence of only the 977bp band was considered indicative of female sex. For DNA Extraction from whole Blood use CinnaGen DNGTM (616101435) and other Samples (Blood, Tissues, biopsy or paraffin-embedded tissue) use CinnaGen DNPTMKit (616201303). Agarose

9

MR7720 Agarose MR7730C AgaroseTopVision LM GQ R0801 (Low Melting) Ethidium Bromide MR7721 Ethidium Bromide MR7729C 66

10g

416101352

50g

416201352

25g

216202353

1ml

316002426

250mg

516001426

TBE Buffer, 5X MR7725C 50 ml 616201419

DNA Ladders & Markers:

GeneRulerTM 50bp, 50-1000 bp SM0371 50µg 316002139

GeneRulerTM 50bp, 50-1000 bp, Ready-to-use SM0373 50µg 316202139

GeneRulerTM 1kb, 250-10000bp SM0311 250 (5x50) µg 316002138

GeneRulerTM 1kb, 250-10000bp , Ready-to-use SM0313 250(5x50) µg 316302138

GeneRulerTM 100bp Plus, 100-3000bp SM0321 50µg 316002137

9

GeneRulerTM 100bp Plus, 100-3000bp, Ready-to-use SM0323 50µg 316202137

GeneRulerTM 100bp, 80-1000bp SM0241 50µg 316002136

GeneRulerTM100bp , 80-1000bp, Ready-to-use SM0243 50µg 316202136

67

10 Clinical Chemistry Kits

Clinical Chemistry Kits:

Albumin (Automatic and manual) BK8144C 300 Tests 500301359

Calcium (Automatic and manual) BK7841C 60 Tests 500101432

Cholesterol (Automatic and manual) BK8043C Creatinine (Automatic) BK8254C 300 Tests 400301375 300 Tests 500301368

Bilirubin, Direct (Automatic and manual) BK8253C 300 Tests 400301363

Bilirubin, Total (Automatic and manual) BK8251C 300 Tests 500301364

Glucose (Automatic and manual) BK8042C Phosphorous (manual) BK7840C 100 Tests 400201404 300 Tests 500301381

10

Phosphorous UV (Automatic and manual) BK8252C 300 Tests 500301405

Total Protein (Automatic and manual) BK8145C 300 Tests 500301421 69

Triglycerides (Automatic and manual) BK8148C 300 Tests 500301422

Urea (Automatic and manual) BK8146C 300 Tests 500301424

Uric Acid (Automatic and manual) BK8147C Ice gel Hard Ice gel SOFT 300 Tests 500g 500g 500301425 300349386 300349475

Urea Diagnostic Kit

- Description: Urea Diagnostic Kits are used for measurement of Urea level in biological fluids such as serum, plasma and urine. Urea measurement is accomplished by a colorimetric method. Urea in biological fluids reacts with Urease enzyme forming a colored complex. Reduction of this compound produces a green-colored complex.

10

The optical density of produced color has a direct relationship with Urea concentration in the solution. CinnaGen's Urea Diagnostic Kit is available in both manual and automated forms. - Specifications: * Measurement by the colorimetric method * Linearity up to 200 mg/dl * Volume sample of 10 µl * Measurement at visual wavelength (578nm) * Available in manual and automated forms * Compatible with the most available commercial control serum * Urea measurement in serum, plasma and urine 70

Cholesterol Diagnostic Kit: An Enzymatic Endpoint Method

- Description: Biochemical Cholesterol Kit is used for measuring cholesterol level in clinical samples such as serum, heparinized plasma and EDTA-treated plasma. - Assay principle: Under influence of cholesterol-oxidase and cholesterol-esterase enzymes, cholesterol in clinical samples generates a red color that its intensity is directly proportional to cholesterol level. - Method: CinnaGen's Cholesterol kit is an enzymatic, colorimetric, endpoint method for both manual and automated forms. - Advantages: Linearity up to 750 mg/dl Low sample volume (10 µl) Visible wavelength 520 nm (500-540 nm) High specificity High stability at room temperature

10

Total Protein Diagnostic Kit

- Description: Total Protein Diagnostic Kit is used for measurement of Total Protein level in biological fluids such as serum and plasma. Total Protein measurement is accomplished by a colorimetric method. Peptide bonds of serum proteins react with Copper ions in alkaline biuret reagent to produce a blue colored complex. The optical density of produced color has a direct relationship with Total Protein concentration in the solution. 71

CinnaGen's Total Protein Diagnostic Kit is available in both manual and automated forms. - Specifications: * Measurement by the colorimetric method * Linearity up to 12 g/dl * Volume sample of 50µl * Measurement at visual wavelength (550nm) * Available in manual and automated forms * Compatible with the most available commercial control serum * Total Protein measurement in serum and plasma

Glucose Diagnostic Kit PAD / GOD Method

- Description: Biochemical glucose kit is used for measuring glucose level in clinical samples such as serum, heparinized plasma and EDTA-treated plasma. - Assay principle: Under influence of Glucose-oxidase and peroxidase enzymes, D-glucose in clinical samples generates a red color that its intesity has a direct relationship with glucose level.

10

- Method: CinnaGen's Glucose Kit is an enzymatic,colorimetric,endpoint method for both manual and automated forms. - Advantages: * Linearity up to 400 mg/dl * Low sample volume (10 µl) * Wavelength 520 nm (500-540 nm) * High specificity * High stability at room temperature - Biochemical kits exported to: Pakistan, India, Australia, Dominican, Malaysia, UK and Bangladesh. 72

Blood Group Typing 11

Blood Group Typing

Blood Group Typing

CinnaCloneIITM Anti-A, B, D (IgG & IgM mono. Ab.) CA7800C 3×10 ml Kit 500201371

CinnaCloneIITM Anti-A antibody (mono. Ab.) CA7801C 10ml 500101370

CinnaCloneIITM Anti-AB antibody (mono. Ab.) CA7805C 10 ml 500101433

CinnaCloneIITM Anti-B antibody (mono. Ab.) CA7802C 10 ml 500101372

CinnaCloneIITM Anti-D antibody IgG & IgM (mono. Ab.) CA7804C 10 ml 500101369

CinnaCloneIITM Anti-D antibody

11

IgM (mono. Ab.) CA7803C 10 ml 500101360

CinnaCloneIITM Anti-D antibody IgG (mono. Ab.) CA8207C Anti-Human Globulin CA8006C 74 10 ml 500101362 10 ml 500101434

ABO-Rh Blood Typing Reagents CinnaCloneIITM Reagents for ABO Typing

-The monoclonal Anti-A, Anti-B and Anti AB reagents are murine monoclonal antibodies of IgM class secreted by mouse hybridoma cell lines. The monoclonal Anti AB reagent is a blend of monoclonal Anti-A and Anti-B antibodies. The noted monoclonal antibodies were selected for their ability to agglutinate specifically human RBCs bearing blood group antigens A and B on direct agglutination tests (slide, microplate and tube test). The reagents do not demonstrate any cross reactivity and do not induce any non-specific agglutination. -8 years in market -Exported to Portugal, Dominican, Turkey, Pakistan, Oman, India, Bangladesh, Kenya and Sri Lanka

Anti-A

Appearance: Specificity: Titer: Transparent blue liquid Does not agglutinate B and O erythrocytes Not lower than 1/64 on tube test

Anti-B

Appearance: Specificity: Titer: Transparent yellow liquid Does not agglutinate A and O erythrocytes Not lower than 1/64 on tube test

11

Anti-AB

Appearance: Specificity Titer: Transparent colorless liquid Does not agglutinate O erythrocytes Not lower than 1/64 on tube test 75

CinnaCloneIITM reagents for Rh typing

The monoclonal Anti-D reagents are human monoclonal antibodies of IgM and IgG classes secreted by chimeric human cell lines. CinnaClone Anti-D IgM consists of one human IgM monoclonal antibody and CinnaClone Anti-D blend is a mixture of human monoclonal antibodies, from both IgG and IgM classes. The noted monoclonal Anti-D reagents were selected for their ability to agglutinate specifically human RBCs bearing Rh antigen D on direct agglutination tests (slide, microplate and tube test). The monoclonal Blend Anti-D may be used in indirect antiglobulin test (Coomds test) due to incomplete IgG components. The reagents do not demonstrate any cross reactivity and do not induce any non-specific agglutination of D negative erythrocytes. - 6 years in market - Exported to Pakistan, Turkey, Oman, India, Portugal, Dominican and Bangladesh Appearance: Specificity: Titer of IgM: Titer of IgG: seconds by slide test. Transparent liquid Does not agglutinate D-negative erythrocytes Not lower than 1/16 on tube test Not lower than 1/32 on tube test

Hemagglutination activity shows at least 2+ agglutination at less than 30

11

Goat Anti-Human Globulin

- Anti-Human Globulin is an antibody produced by goat in response to deliberate immunization with purified human immunoglobulins or complements (primarily beta globulins). Anti-Human Globulin is used in the antiglobulin test that is a sensitive technique used to detect human immunoglobulins and/or complement components bound to human 76

erythrocytes. Anti-Human Globulin is used in Direct Antiglobulin Test (DAT) to detect antibodies and/or complements bound to red cells in vivo. Direct Antiglobulin Test results may support the diagnosis of AutoImmune Hemolytic Anemia (AIHA), Hemolytic Disease of the Newborn (HDM) and Delayed Hemolytic Transfusion Reactions (DHTR). Indirect Antiglobulin Test (IAT) detects immunoglobulins and/or complements bound to red cells in vitro and also identifies unexpected blood group antibodies and red cell phenotyping. - 6 years in market - Exported to Turkey, Oman, Pakistan and Bangladesh

11

77

12 Cell Culture

Cell Culture

CHO-S-SFM II (w/o) HT 31033-020 500 ml 200329870

D-MEM/F-12 (1:1) (1X) liquid 21331-046 New Born Calf Serum 16010159 500 ml 315329465 500 ml 200329437

Fetal Bovine Serum Origin: EU Approved (South American) 10270-106 500 ml 315329464

Fetal Bovine Serum - Origin: France, EC Approved 10106-169 500 ml 315329464

L-Glutamine 200 mM (100X), liquid 25030-024 Trypsin ­EDTA (1X) 25300-054 100ml 100 ml 315229654

Trypsin- EDTA without calcium and magnesium CR7911 10 ml 515101460

12

Bovine serum Albumin, Solution at 30% K15-018 500 ml 224334039

79

Trypsin 0.25% (W/V) without calcium and magnesium CR7910 10 ml 515101838

RPMI 1640 W/25MM HEPES 13018031 10 l 215329411

RPMI Medium 1640 (1X), liquid 21875034 Streptomycin 200 mg/ml CR7912 Penicillin 200,000 u/ml CR7913 5 ml 515101463 5 ml 515101461 500 ml 215429411

Trypsin 0.25% (W/V) without calcium and magnesium

12

80

ELISA Based Diagnostic Kits 13

ELISA Based Diagnostic Kits

ELISA Kits

HBs CinnaScreenTM EL8002C HCV CinnaScreenTM EL8003C 96 Tests 518001456 96 Tests 518001452

HCV CinnaScreenTM

- Enzyme linked Immunosorbent assay for detection of antibodies to Hepatitis C virus is a major cause of acute and chronic liver disease including Cirrhosis and Hepatocellular cancer. The prevalence of HCV infection is increasing worldwide. A mixture of highly purified recombinant and synthetic (NS3, NS4, NS5 and core) is used for detection of anti-HCV antibodies in human sera. - Advantages: High Sensitivity (100%) and Specificity Comply with BBI panels including PHV 905, PHV 906, PHV 914, WWH V301, PHV 104, PHV 908, PHV 105M and PHV 205 Short incubation time required

13

Designed and up-to-dated with last generation of ELISA techniques. - Exported to India, Pakistan, Turkey, Kenya and Bangladesh.

82

HBs CinnaScreenTM

- Enzyme-linked Immunosorbent assay for detection of Hepatitis B surface antigen (HBs Ag). Hepatitis B is an infectious liver disease caused by hepatitis B virus (HBV). The diagnosis of HBV infection is made on the basis of serology. - Advantages: The assay is based on the detection of surface antigen in individual sera. Short incubation time. It detects as low as 0.1 ng of surface antigen in sample sera. The HBs CinnaScreen is a single step ELISA Kit. - Exported to India, Pakistan, Kenya and Bangladesh.

13

83

14 Generic Peptides

Generic Peptides & APIs

Luliberin A (GnRH Analog) SP8001C Buserelin SP8003C 1g 513001367 1g 513101388

Cloprostenol Na (Prostaglandin F2a Analog) SP8002C 1g 500101453

Buserelin

Buserelin acetate is an analogue of Gonadorelin with similar properties. It is used for the suppression of testosterone in the treatment of malignant neoplasms of the prostate; it is also used in the treatment of endometriosis and as an adjunct to ovulation induction with gonatrophins in the treatment of infertility. It has also been used in precocious puberty and has been tried in the treatment of uterine fibroids.

GnRH- A (Luliberin-A)

Luliberin-A is an analogue of Gonadorelin with similar properties for veterinary use. It is used for the fertility disorders by ovarian disfunction, fixing time of ovulation, prophylaxis of fertility disorders by early induction of cycle postpartum and improvement of conception rate.

14

85

Sodium Cloprostenol

Cloprostenol is an analogue of prostaglandin F2a for veterinary use. It is used for induction and synchronization of heat in heifers and cows, synchronization of oestrus cycle in donor, treatment of functional disorders of ovaris and termination of normal or pathologic pregnancy of the first half of pregnancy.

Vet Products

CinnaProstTM (Sodium Cloprostenol) Cinnaprost is approved for use in beef or dairy cattle to induce luteolysis. CinnaRelinTM (GnRH-A) This product is indicated for the treatment of ovarian cysts in dairy cattle. CinnoTalTM (Buserelin acetate) It is approved for the treatment of infertility of ovarian origin and improvement of pregnancy rate in cows and also for the synchronization of oestrus in dairy cows.

14

86

Alphabetical Index

ALPHABETICAL INDEX

A B C D E F G H I K L M N O P Q R S T U V W X Y Z

A

Acrylamide .................................................... 56 Agarase ..................................................... 20-49 Agarose ......................................... 32-43-56-66 AhaIII (see DraI) ............................................ 5 Albumin ......................................................... 69 Albumin see Bovine Serum Albumin ...... 28-79 Alkaline phosphatases: · Bacterial Alkaline Phosphatase ........... 19 · Calf Intestine Alkaline Phosphatase .... 19 · Shrimp Alkaline Phosphatase ............ 19 Alu .................................................................... 4 Ampicillin E.coli FastMediaTM ...................... 40 AMS Buffer see 10X PCR Buffer with (NH4)2SO4 ..................................................... 28 Anti-Human Globulin ............................ 74-76 AvaII see Eco47I .............................................. 4 · E.coli XL1-Blue ................................... 38 BamHI .............................................................. 4 BclI .................................................................... 4 BglI .................................................................... 4 BglII .................................................................. 5 Bilirubin ......................................................... 69 Biochemical Kits · Albumin ............................................... 69 · Bilirubin, Direct .................................. 69 · Bilirubin, Total ................................... 69 · Calcium ................................................ 69 · Cholesterol .......................................... 69 · Glucose ................................................. 69 · Phosphorous (manual) ....................... 69 · Phosphorous UV ................................. 69 · Total Protein ......................................... 69 · Triglycerides ......................................... 70

B

Bacterial Alkaline Phosphatase ................... 19 Bacterial Media ............................................. 40 Bacterial Strains · E.coli BL-21 (DE3) ............................. 38 · E.coli DH5a ......................................... 38 · E.coli HB101 ....................................... 38 · E.coli TGI ............................................ 38

· Urea ....................................................... 70 · Uric Acid .............................................. 70 Bis Acrylamide .............................................. 56 Blood Group Typing · CinnaCloneIITMAnti-A antibody ..... 74 · CinnaCloneIITM Anti-B antibody ..... 74 · CinnaCloneIITM Anti-AB antibody .... 74 · CinnaCloneIITM Anti-A, B, D ........... 74

88

· CinnaCloneIITM Anti-D antibody IgG .... 74 · CinnaCloneII

TM

Calf Intestine Alkaline Phosphatase ........... 19 cDNA · RevertAidTM First Strand cDNA

Anti-D antibody-

IgG & IgM ............................................ 74 · CinnaCloneII

TM

Anti-D antibody-

Synthesis Kit ........................................ 29 Cell Culture Reagents · Bovine Serum Albumin, Solution at 30% ........................................................... 79 · CHO-S-SFM II (w/o) HT .................... 79 · D-MEM/F-12 ..................................... 79 · Fetal Bovine Serum ............................ 79 · L-Glutamine ........................................ 79 · Newborn Calf Serum ........................ 79 · Penicillin ............................................... 80 · RPMI 1640 ............................................ 80 · RPMI Medium 1640 (1X), Liquid ..... 80 · Streptomycin ............................................ 80 · Trypsin 0.25% (W/V) without calcium and magnesium ......................................... 80 · Trypsin- EDTA (1X) .................................. 79 · Trypsin- EDTA without calcium and magnesium ............................................. 79 Cholesterol ................................................... 69 CHO-S-SFM II (w/o) HT .......................... 79 CinnaCloneIITM Anti-A antibody ................. 74 CinnaCloneIITM Anti-B antibody ................ 74 CinnaCloneIITM Anti-AB antibody .............. 74 CinnaCloneIITM Anti-A, B, D ....................... 74 CinnaCloneIITM Anti-D antibody IgG .......... 74

IgM .......................................................... 74 Boric Acid ...................................................... 56 Bovine Serum Albumin ......................... 28-79 5-bromo-4-chloro-3-indoly l-b et a-Dgalactopyranoside see X-Gal ....................... 37 5-bromo-4-chloro-3-indoly l-b et a-Dglucuronic acid see X-Gluc ......................... 40 BspCI see PvuI ................................................ 9 BsuRI (see HaeIII) .......................................... 6 Buffers for DNA, RNA & protein

electrophoresis · Loading Buffer Pack ........................... 56 · 6X Loading Dye Solution ...... 32-42-58 Buffers for Electrophoresis · 5X TBE Electrophoresis Buffer .............. .............................................. 33-43-58-67 Buffers for PCR · 10X PCR Buffer with KCl .................. 27 · 10X Taq Buffer with KCl ................... 27 · 10X PCR Buffer with (NH4)2SO4 (see AMS Buffer) ................................... 28

Buserelin ................................................. 85 Buserelin acetate .................................. 86

C

Calcium .......................................................... 69

CinnaCloneIITM Anti-D antibody IgG & IgM ................................................................ 74

89

CinnaCloneIITM Anti-D antibody IgM .......... 74 CinaProst

TM

DNA Polymerase I Large Fragment (Klenow Fragment) ...................................................... 15 DNA Polymerase I Large Fragment, Exonuclease Minus ........................................ 15 DNA Purification see GeneJETTM Plasmid Miniprep Kit .................................................. 45

.................................................... 86 ................................................ 86

CinnaRelin CinnoTal

TM

TM TM

.................................................... 86

CinnoVex ....................................................... 2 Cloning · GeneJET

TM

PCR Cloning Kit ............... 36

TM

DNA Topoisomerase I ................................. 20 dNTP · dNTP/dUTP .................................. 27-60 · dNTP Mix, 10mM each ................. 27-60 · dNTP Set ........................................ 27-60 · dUTP ............................................... 27-60 DraI (AhaIII) ................................................. 5 DTT .................................................... 29-41-48

· InsTAclone PCR Cloning Kit ............ 36 · Vectors and Phage DNA .................... 39

Cloprostenol Na (Prostaglandin F2a Analog) .................................................. 85 Creatinine ................................................ 69 Cytomegalovirus PCR Detection Kit ...... 63

D

Deoxyribonuclease I (DNase I), RNase-free ... 17 DEPC ....................................................... 28-48 DEPC-treated Water .............................. 28-48 D-MEM/F-12 ............................................... 79 DMSO .......................................................... 41 DNA electrophoresis ........................... 32-41-56 DNA Extraction Kit (from Agarose gels) ....... 46 ·DNA Extraction Kit (see DNP Kit) ...... 46 ·DNA Extraction Solution (see DNG ) .. 47 · DNA Purification Kit ............................ 47 DNA Ladders (see GeneRuler) ....... 33-41-57-67 DNA Markers (see Marker) .......... 33-41-55-57 DNA polymerase see Polymerases ................. .................................................. 13-15-16-23-24 DNA Polymerase I, E.coli ............................. 15

TM TM

E

E. Coli · E.coli BL-21 (DE3) .............................. 38 · E.coli DH5a ......................................... 38 · E.coli HB101 ......................................... 38 · E.coli TGI ............................................ 38 · E.coli XL1-Blue ................................... 38 Eco32I ............................................................... 5 EcoRI ................................................................. 5 EcoRV see Eco32I .......................................... 5 Eco47I (seeAvaII) ............................................ 4 EDTA, (Titriplex III) ................................... 50 EDTA, 0.5mM, pH 8.0 ................................. 50 Electrophoresis Buffers ·5XTBEElectrophoresisBuffer...33-43-58-67

90

Elisa Kits ...................................................... 82 Ethidium Bromide ................. 32-42-43-57-66

250-10000bp ................................. 33-41-57-67 GeneRulerTM 1kb DNA Ladder, 250-10000bp, ready-to-use ................................ 33-41-57-67

F

FastDigest ....................................................... 11 FastMedia

TM TM TM

GeneRulerTM Mix

DNA

Ladder,

100-

10000bp ........................................... 34-42-58 GeneRulerTM Mix DNA Ladder, 100-

LB Agar Amp ........................ 40 LB Agar Amp IPTG/X-Gal ..... 40 LB Liquid Amp ........................ 40

TM

FastMedia FastMedia

10000bp, ready to use ..................... 34-42-58 Genomic DNA Purification Kit · DNA Extraction Kit (see DNPTM Kit) ..... 46 ·DNA Extraction Solution (see DNGTM) .. 47 Glucose ........................................................... 69 Glycogen ....................................................... 49 Glycosylase see Uracil-DNA Glycosylase ....... 21

Fast PCR see PyroStart

Fast PCR Master

Mix (2X) ....................................................... 26 Fetal Bovine Serum .................................. 79 First Strand cDNA Synthesis Kit ............ 29

G

GeneJET GeneJET

TM TM

PCR Cloning Kit ................... 36 Plasmid Miniprep Kit ............... 45

H

HaeIII see BsuRI ........................................... 6 HBs CinnaScreenTM .................................. 82 HCV CinnaScreenTM ................................ 82 Helicobacter pylori PCR Detection Kit .... 64 Hepatitis B Virus PCR Detection Kit ........ 62 Herpes Simplex virus I&II Detection Kit ..... 63 HhaI (see Hin6I) ....................................... 6 High Fidelity PCR Enzyme Mix ............... 24 HincII (see HindII) .................................... 6 Hin6I (see HhaI) ......................................... 6 HindII (see HincII) ...................................... 6 HindIII .......................................................... 6 HinfI .............................................................. 7 Hot Start Taq DNA Polymerase ........... 13-23 HpaII (see MspI) ..................................... 7

Generic Peptide ............................................ 85 GeneRulerTM 50-1000bp DNA Ladder .......... ........................................................ 33-41-57-67 GeneRuler

TM

50-1000bp

DNA

Ladder,

ready-to-use ................................. 33-41-57-67 GeneRuler

TM

100bp Plus, 100-3000bp DNA

Ladder .................................... 33-41-42-57-67 GeneRuler

TM

100bp Plus, 100-3000bp DNA

Ladder, ready-to-use ................ 33-41-42-57-67 GeneRuler

TM

100bp,

80-1000bp

DNA

Ladder ......................................... 33-42-58-67 GeneRuler

TM

100bp, 80-1000bp DNA Ladder

DNA Ladder, ready-to-use ............ 33-42-58-67 GeneRuler

TM

1kb

DNA

L adder,

91

I

Ice gel Hard .............................................. 70 Ice gel Soft ................................................. 70 InsTAcloneTM PCR Cloning Kit ............... 36 IPTG ........................................................... 37 Isopropyl-beta-D-thiogalactopyranosidase see IPTG ....................................................... 37

·PageRuler TM

Unstained

Protein

Ladder ................................................... 55 Lambda DNA .............................................. 39 Lambda DNA/EcoRI+HindIII Marker

3, (125-21226)bp ............................... 34-42-58 Leishmania sp. PCR Determination and Detection Kit ................................................ 65 L-Glutamine .............................................. 79

K

Kinase ......................................................... 19 Kinase see T4 Polynucleotide Kinase ........... 20 Kits for Molecular Biology: · First strand cDNA synthesis ........... 29 · GeneJet PCR Cloning Kit ............... 36 · Ins TA clone PCR Cloning Kit ......... 36 · Kits for cloning .................................... 36 · Kits for purification ............................. 47 · Kits for detection ................................ 62 · Kits for transcription ......................... 52 Klenow Fragment ....................................... 15 Klenow Fragment, exo- .......................... 15 KpnI ............................................................... 7

Ligases, ligation: · T4 DNA Ligase .................................... 19 · T4 DNA Ligase (with PEG) ................. 19 · T4 RNA Ligase .................................... 19 6X Loading Buffer ........................ 32-42-58 Loading Buffer Pack ................................... 56 Long PCR Control Set ................................. 26 Long PCR Enzyme Mix ............................ 25 Luliberin A (GnRH Analog) ...................... 85 Lysozyme .................................................... 50

M

Markers and Ladders · L amb d a DNA / E c o R I Hi n d I I I Marker, 3 ..................................... 34-42-58

L

Ladders ................................ 33-41-55-57-67 Ladder (see GeneRuler) · PageRuler TM Prestained Protein Ladder .................................................... 55 · PageRuler TM Prestained Protein Ladder Plus ............................................. 55

· PageRuler TM Prestained Protein Ladder .................................................. 55 · PageRuler TM Prestained Protein Ladder Plus ........................................... 55 · PhiX174 DNA/BsuRI (HaeIII) Marker, 9 ..................................... 34-42-58

· PhiX174 DNA/BsuRI (HaeIII)

92

Marker, 9, ready-to-use ............. 34-42-58

· Ribonucleases (RNases) ..................... 18 · Ribonuclease A (RNase A), DNase-free .................................................................. 18 · Ribonuclease T1 ................................... 18 · RNase A/T1 Mix ................................. 18 Nucleotides .............................................. 26-60

· Prestained Protein Molecular Weight

Marker .................................................... 55

· Unstained Protein Molecular Weight

Marker .................................................... 55 Master Kit ............................................... 25 MboII .............................................................. 7 Medium Run (see sequencing service) .......... .................................................................... 32-43 Mesophilic DNA Polymerases .................. 15 MgCl2, 50mM ............................................ 28 Microbiological Media ................................ 40 Miniprep purification see GeneJET

TM

O

Oligodt 18 ..................................................... 31

P

PageBlue

TM

Protein Staining solution ..... 55 Prestained Protein Ladder ...... 55

Plasmid

PageRuler

TM

Miniprep Kit .................................................. 45 Mineral Oil ................................................. 34 M-MuLV Reverse Transcriptase .......... 16-29 MspI (see HpaII) ............................................ 7 Mycobacterium tuberculosis PCR Detection Kit .................................................................. 62

PageRuler Prestained Protein Ladder Plus .. 55 PageRulerTM Unstained Protein Ladder ...... 55 pBR322 DNA ........................................ 38-39 PCR: · Check System ..................................... 26 · Detection Kits .................................... 62 · DNA Extraction Kit & Solution ...... 45-46-47

TM

N

NcoI .............................................................. 8 NdeI .............................................................. 8 Neisseria gonorrhoeae PCR Detection Kit .......................................................................... 65 Newborn Calf Serum .............................. 79 NotI ................................................................... 8 Nucleases: · Deoxyribonuclease I (DNase I), RNase-free .............................................. 17

· dNTP/dUTP mix ......................... 27-60 · dNTP Mix, 10mM ........................ 27-60 · dNTP set ..................................... 27-60 · dUTP ........................................... 27-60 · First Strand cDNA Synthesis Kit ..... 29 · GeneJETTM PCR Cloning Kit ................ 36 · Genomic DNA Purification Kit ........ 37 · High Fidelity PCR Enzyme Mix ....... 24 · Hot Start Taq DNA Polymerase ...... 13-23 · InsTAcloneTM PCR Cloning Kit ............ 36

93

· Long PCR Control Set ................... 26 · Long PCR Enzyme Mix .................... 25 · Master Kit (see 2X PCR Master Mix) ..... 25 · MgCl2 50mM ....................................... 28 · Mineral Oil .......................................... 34 · PCR Buffer with KCl, 10X ............ 27 · PCR Buffer with (NH4)2SO4 (see AMS Buffer), 10X ............................................ 28 · PCR Master Mix, 2X ...................... 25 · Pfu DNA Polymerase ................. 13-24 · PyroStart FastPCRMasterMix(2X).... 26 · RevertAid

TM TM

pGEM-7Zf(-) ................................................ 38 pGEM-9Zf(-) ................................................ 38 Phenol Equilibrated ...................................... 48 PhiX174DNA/BsuRI (HaeIII) Marker 9, 72-1353bp .......................................... 34-42-58 PhiX174DNA/BsuRI (HaeIII) Marker 9, 72-1353bp, ready to use ................... 34-42-58 Phosphatases: · Bacterial Alkaline Phosphatase .......... 19 · Shrimp Alkaline Phosphatase.......... 19 · Calf Intestine Alkaline Phosphatase ... 19 Phosphorous .................................................. 69 Phosphorous UV ........................................... 69 Phosphorylation: · T4 Polynucleotide Kinase .................. 20 Plasmids · pBR322 DNA 38-39 · pGEM-3Z .............................................. 38 · pGEM-4Z .............................................. 38 · pGEM-3Zf(-) ......................................... 38 · pGEM-5Zf(+) ....................................... 38 · pGEM-5Zf(-) ....................................... 38 · pGEM-7Zf(+) ...................................... 38 · pGEM-7Zf(-) ........................................ 38 · pGEM-9Zf(-) ........................................ 38 · pSP 70 ................................................... 38 · pSP 71 .................................................... 38 · pSP 72 ..................................................... 38 · pSP 73 .................................................... 38 · pTZ19R DNA ................................. 38-39

First

Strand

cDNA

· Synthesis Kit ........................................ 29 · Set System ........................................... 25 · SmarTaq DNA Polymerase ......... 13-23 · Taq Buffer with KCl, 10X .......... 27 · Taq DNA Polymerase (recombinant) ....................................................... 14-23-24 · Taq DNA Polymerase (native,without BSA) .................................................... 14-24 · Water, nuclease-free ........................... 28 Penicillin 200,000 u/ml ................................ 80 Peptide (see Generic Peptide) .................. 85 Pfu DNA Polymerase: recombinant ........... 13-24 pGEM-3Z ...................................................... 38 pGEM-4Z ....................................................... 38 pGEM-3Zf(-) ................................................. 38 pGEM-5Zf(+) ................................................ 38 pGEM-5Zf(-) ................................................. 38 pGEM-7Zf(+) ............................................... 38

94

· pUC18 DNA .................................. 38-39 · pUC19 DNA ................................. 38-39 · pUC57 DNA .................................. 38-39 Plasmid DNA Extraction kit ................... 45 Plasmid DNA purification see GeneJET

TM

Protein Electrophoresis · Reagent for ................................... 55 Protein ladders and markers: · PageRulerTM Prestained Protein Ladder ................................................... 55 · PageRulerTM Prestained Protein Ladder Plus ..................................... 55 · PageRulerTM Unstained Protein Ladder .............................................. 55 · · Prestained Unstained Protein Protein Molecular Molecular Weight Marker ................................. 55 Weight Marker .............................. 55 Protein Standard Solution ................ 55 pSP 70 ................................................ 38 pSP 71 .................................................. 38 pSP 72 .................................................. 38 pSP 73 ................................................... 38 PstI ......................................................... 8 pTZ19R .............................................. 40 pUC18, pUC19 .............................. 38-39 pUC57 ................................................. 39 PvuI ........................................................ 9 PvuII ...................................................... 9 Pyrophosphatase, Inorganic (from yeast) ........................................................................ 21 P y ro St a r t T M Fa s t PCR Ma s t e r Mix (2X) .................................................. 26

Plasmid Miniprep Kit ................................... 45 Polymerases: · DNA Polymerase I ............................... 15 · DNA Polymerase I Large Fragment (Klenow Fragment) .............................. 15 · DNA Polymerase I Large Fragment, E xonucle as e M i nu s (Klenow

Fragment, exo-) ................................. 15 · Hot Start Taq DNA Polymerase ...... 13-23 · Klenow Fragment ................................. 15 ·Pfu DNAPolymerase: recombinant..13-24 · T4 DNA Polymerase ........................... 16 · T7 DNA Polymerase ............................ 16 · T7 RNA Polymerase ....................... 17-53 · Taq DNA Polymerase (recombinant) .............................................................. 14-23-24 · Taq DNA Polymerase (native): without BSA ..................................................... 14-24 PreStained Molecular Weight Marker ...... 55 Primer · Oligodt 18 ....................................... 31 · Random hexamer ......................... 31 · Set ................................................... 31 · Synthesis ........................................ 31

Proteinase K .................................... 21-49 95

R

Random Hexamer Primer ................ 31 Reagents · For Protein Electrophoresis ......... 55 Restriction Enzymes ............................ 4 Reverse transcriptase see RevertAid RevertAid RevertAid

TM TM

S

SacI ......................................................... 9 Sarcosyl ................................................ 50 SalI ....................................................... 10 SAP see Shrimp Alkaline Phosphatase .. 19 SDS ................................................. 50-56 Sequencing Services .................... 32-43 Sex Determination PCR Kit .............. 65 Short Run (see sequencing) ........... 32-43 Shrimp Alkaline Phosphatase ........... 20 SmaI ..................................................... 10 SmarTaq DNA Polymerase ......... 13-23 Sodium Acetate .................................. 50 Sodium Cloprostenol ........................ 86 Sodium Dodecyl Solfate .............. 50-56 SP6 RNA Polymerase ........................ 53 Streptomycin 200 mg/ml ................... 80 STRPTM Hepatitis C Virus Detection Kit (single tube RT-PCR) .................... 62 STRPTM HIV Detection Kit (single tube RT-PCR) .............................................. 64 Synthesis (see Primer Synthesis) ....... 31

............................................................ 16-29 First Strand cDNA Synthesis Kit ......................................... 29

TM

H Minus First Strand cDNA M-MuLV Reverse

Synthesis Kit RevertAid RevertAid

TM

Transcriptase ................................... 16-29

TM

H Minus M-MuLV Reverse

Transcriptase Ribonucleases ...................................... 18 Ribonuclease A (RNase A), DNase-free (DNase and protease free) ...................... 18 Ribonuclease T1 .................................. 18 RiboLock

TM

Ribonuclease Inhibitor .........

..................................................... 17-30-53 Ribonuclease Inhibitors ......... 17-30-53 RNA polymerases see Polymerases ............................................................. 17-53 RNase A/T1 Mix ................................ 18 RNX

TM

T

10X Taq Buffer with KCl ................... 27 5X TBE Electrophoresis Buffer ............. ............................................. 33-43-58-67 T3 RNA Polymerase ......................... 53 T4 DNA Ligase ................................. 19 T4 DNA Ligase (with PEG) ............. 19

plus

solution

(for

RNA

Extraction) ............................................ 48 RPMI 1640 ......................................... 80 RsaI ......................................................... 9

96

T4 DNA Polymerase ........................... 16 T4 Polynucleotide Kinase ................. 20 T4 RNA Ligase .................................. 19 T7 DNA Polymerase .......................... 16 T7 RNA Polymerase ...................... 17-53 T7 Transcription Kit .......................... 52 TaqI ....................................................... 10 Taq DNA Polymerase (recombinant) .... ...................................................... 14-23-24 Taq DNA Polymerase (native, without BSA) .................................................. 14-24 Thermophilic DNA Polymerases ........ 13 Titriplex III (EDTA) ........................... 50 Topoisomerase (see DNA TopoisomeraseI) ................................................................. 20 TopVisionTM LE GQ Agarose ...... 32-43-57-66 Total Protein ........................................ 69 Transcriptase see M-MuLV Reverse Transcriptase .................................... 16-29 Transcription see T7 Transcription Kit ... 52 · Enzyme for Transcription .......... 52 Triglycerides .......................................... 70 Tris Base ................................................. 50 Trypsin 0.25% ........................................ 80 Trypsin- EDTA ...................................... 79

Urea ................................................. 70 Uric Acid .......................................... 70

V

Vet Products ......................................... 86

W

Water, DEPC-treated ............... 28-40 Water, nuclease-free .......................... 28

X

XbaI ..................................................... 10 X-Gal ................................................... 37 X-Gal Solution, ready-to-use ........... 38 X-Gluc ................................................. 40 XhoI ..................................................... 11

Y

Yeast (see Pyrophosphatase, Inorganic) .... 21

U

Unstained Protein Molecular Weight Marker .................................................. 55 Uracil-DNA Glycosylase ..................... 21 97

Pioneer in Molecular Biology and biotechnology

l CinnoVexTM , the only biogeneric

form of IFN -1a

l Rec

Hu- Erythropoietin as synthetic peptide API (GnRH-A)

l Buserelin

l CinnaRelinTM l

CinnaProstTM (Na Cloprostenol) (Buserelin acetate)

for veterinary use.

l CinnoTalTM

l

CinnaCloneTM, M o n o c l o n a l

Anti-bodies for Blood Group Typing (Anti-A, Anti-B, Anti-AB & Anti-D)

l Restriction enzymes and polymerase

for genetic engineering research

l Oligo

Synthesis

Information

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