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Histology Worksheet

What is histology? Histology is the study of biological tissue at the micro-scale. (Is

that big or small?) Micrometer thick sections of biological samples are treated with staining techniques used to enhance observation and identify particular constituents. (What's a constituent?) Histology is a common tool used to investigate pathology of diseased tissue to ensure correct diagnosis. In our lab, histology is mainly used to determine the structural components in heart tissue. There are many different staining protocols each with specific target constituents or tissues as seen in Figure 1.

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Figure 1 Example Histology Stains a) Myocardium of a dog with dilated cardiomyopathy stained with Masson's trichome b) Mouse kidney section stained with Hematoxylin & Eosin a common structure stain c) Human skin stained using Masson-Trichrome technique(collagen blue) d) Alizarin Red Staining of mouse embryo showing calcium deposition.

Sources: www.vetpathology.org/content/vol42/issue1/cover.shtml www.ihcworld.com/imagegallery/index.php?cat=3

Movat's Pentachrome Movat's Pentachrome is a connective tissue staining procedure that colors the components of the specimen according to the following color scheme: Nuclei...........................................................Black Elastic Fibers.............................Dark Purple to Black Collagen ...........................................Yellow/Pinkish Ground Substance.......................Blue to Bluish Green Fibrinoid................................................Intense Red Muscle.............................................................Red Histology Scavenger Hunt: You have a series of pictures containing cardiac cross-sections that have been stained with Movat's Pentachrome. Using these images, and your measurement software, work through this guided exploration of the heart's microstructure. Keep in mind that these questions are meant to guide your thinking, but be sure to record observations and questions you think of as you go along. Open up the image titled DAY8_HEART_44x. Sketch the heart cross-section and identify the following components: ventricles, atriums, pericardium, atrioventricular valves, outflow tracts. Don't forget to calibrate images so that you get correct measurements!!

Use the measurement tools in ImageJ to quantify the different features of the heart, e.g. wall thickness, compare atrium and ventricle chamber area, etc.

Identify the direction of blood flow in the heart. How do you think the blood flow influences the development of the heart?

Open up the image titled, "Ventricle1_525x". Describe the ventricle wall (be sure to include color, structure, cell density, etc). Considering the function of the ventricle, what are the pros and cons of this design?

From the color scheme, what do you think the outflow tracts are mostly composed of? What do you think the reason for this is?

Open image titled "AV_VALVE_525x", which is a zoomed in picture of the atrioventricular valves. Describe the valve structure.

Based on the color scheme, what are the main constituents that make up the valve?

Using both images DAY8_HEART and VENTRICLE1_525x, measure the thickness of the pericardium (outer layer of ventricle wall). Compare with the thickness of the rest of the wall. Does the wall thickness vary? If so, why do you think this is?

What other observations can you make about the cross-sections?

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Histology - Worksheet

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Histology - Worksheet