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Toxicological data on Melissa EO Melissa Officinalis

CAS : 8014-71-9

Skin Sensitization : LLNA Local Lymph Node Essay (LLNA) (OCDE 429) (Robertet 2008) EC3 calculated = 18% (w/v) 4500µg/cm2 Carrier acetone/Olive oil ( 4:1) Human Human Repeat Insult Patch Test (HRIPT) (Robertet 2001) Test Protocol : Marzulli-Maibach protocol semi-occlusive 50 subjects Test Concentration: 2% (w/w) (825µg/cm2) Carrier Ethanol/DEP (1:3) Conclusion : No induction of sensitization.

Human Repeat Insult Patch Test (HRIPT) (Robertet 2008) Test Protocol : Marzulli-Maibach semi-occlusive 100 subjects Test Concentration: 2 % (w/w) (910µg/cm2 ) Carrier Ethanol/DEP (1:3) Conclusion : No induction of sensitization Human Repeat Insult Patch Test (HRIPT) (Robertet 2009) Test Protocol : Marzulli-Maibach semi-occlusive 100 subjects Test Concentration: 2 % (w/w) (1470µg/cm2 ) Carrier Ethanol/DEP (1:3) Conclusion : No induction of sensitization

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Microsoft Word - Melissa EO Anne marie Api.doc