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SOP: R005 Preparation of Coomassie Destain Solution Materials and Reagents: (per 1L) 1. Graduated cylinders, 1 liter and 250 ml 2. Glass beaker, 1 liter 3. Stir bar 4. Magnetic stir plate 5. Milli-Q water: 750 ml 6. Methanol: 150 ml 7. Acetic acid, glacial: 100 ml 8. Glass bottle or polypropylene storage container, 1 liter Protocol: 1. _____ Combine Milli-Q water, methanol, and acetic acid in glass beaker and stir on a stir plate for approximately 5 minutes. 2. _____ Transfer to storage container. References: Coligan, J.E. et al. Current Protocols in Protein Science. Volume 2. pp 10.5.10

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