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PUNJAB TECHNICAL UNIVERSITY Jalandhar

DISTANCE EDUCATION PROGRAMME http://www.ptu.ac.in

Syllabus Manual

Paramedical Technology Courses

Applicable to September 2005 Admission Batch

© Copyright, Punjab Technical University

Contents Scheme Dip. In Operation Theatre Technology (DOTT) ............ Diploma in Medical Lab Technology (DMLT) ................ Diploma in Radiology & Imaging Technology (DRIT) ..... B.Sc. (Biotechnology) (BSCBT)................................ B.Sc. (Medical Lab Technology) (BSCMLT).................. B.Sc. (Clinical Microbiology) (BSCCMB)...................... B.Sc. (Bioinformatics) (BSCBI) ................................. M.Sc. (Medical Lab Technology) (MSCMLT)................. M.Sc. (Biotechnology) (MSCBT)............................... M.Sc. (Clinical Microbiology) (MSCCMB) .................... M.Sc. (Bioinformatics) (MSCBI)............................... Page No. Syllabus Diploma in Operation Theatre Technology (DOTT) ........ Diploma in Radiology & Imaging Technology (DRIT) ...... Diploma in Medical Lab Technology (DMLT) ................ B.Sc. (Medical Lab Technology) (BSCMLT)................... B.Sc. (Clinical Microbiology) (BSCCMB)...................... B.Sc. (Biotechnology) (BSCBT)................................. B.Sc. (Bioinformatics) (BSCBI) ................................. M.Sc. (Medical Lab Technology) (MSCMLT)................. M.Sc. (Biotechnology) (MSCBT)............................... M.Sc. (Clinical Microbiology) (MSCCMB) .................... M.Sc. (Bioinformatics) (MSCBI)............................... 01-10 11-22 23-30 23-35 36-55 56-76 77-95 96-107 108-120 121-135 136-144

PUNJAB TECHNICAL UNIVERSITY Jalandhar

DISTANCE EDUCTION PROGRAMME http://www.ptu.ac.in

Scheme

September 2005

© Copyright, Punjab Technical University

Scheme of Papers Diploma in Operation Theatre Technology (DOTT) Applicable to Septemebr 2005 Admissions onward Semester One Contact Hours (PerSemester) Practical Hrs Theory Hrs Theory Exam Marks Practical Exam Marks Duration of Exams Remarks

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 DOTT-101 2 DOTT-102 3 DOTT-103 4 DOTT-104 5 DOTT-105 6 DOTT-106 7 DOTT-107

PaperName Anatomy & Physiology Pathology & Microbiology Surgical Equipment & Machinery Anesthesia Equipments and Drugs Communication & Soft Skills Introductoru Biology Practical Total

40 30 20 40 20 20 -

10 10 20 10 10 10 -

50 40 40 50 30 30 240

25 25 25 25 25 -

75 75 75 75 75 -

100 100 100 100 100 500

50 25

50 100 150

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Practical 650 Total Duration of Exams

Semester

Two

Contact Hours (PerSemester) Practical Hrs Theory Hrs

Theory Exam Marks

Practical Exam Marks

Remarks

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 DOTT-201 2 DOTT-202 3 DOTT-203 4 DOTT-204 5 DOTT-205

PaperName Medicine Surgery Obtretics & Gyneacology Eye & ENT Practical Total

40 40 40 40 160

10 0 10 10 30

50 40 50 50 190

25 25 25 25 -

75 75 75 75 -

100 100 100 100 400

25

100 100

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Practical 500 Total

Page 1 of 1

Scheme of Papers Diploma in Medical Lab Technology (DMLT) Applicable to Septemebr 2005 Admissions onward Sr Semester One Contact Hours (PerSemester) Practical Hrs Theory Hrs Theory Exam Marks Practical Exam Marks Duration of Exams Remarks

Total Hrs

External

External 0 75

Internal

Internal

Total

Sr PaperCode 1 BSCMLT-101 2 BSCMLT-102 3 BSCMLT-103 4 BSCMLT-104 5 BSCMLT-105 6 BSCMLT-106 7 BSCMLT-107

PaperName Microbiology Anatomy and Histotechnology Hematology and Clinical Pathology Biochemistry Introductory Biology Communication & Soft Skills Practical Total

40 30 40 30 20 20 180

10 10 10 10 10 10 60

50 40 50 40 30 30 240

25 25 25 25 25 -

75 75 75 75 75 -

100 100 100 100 100 500

50 25

50 100 160

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Practical Practical 650 Total Duration of Exams

Sr Semester

Two

Contact Hours (PerSemester) Practical Hrs Theory Hrs

Theory Exam Marks

Practical Exam Marks

Remarks

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 BSCMLT-201 2 BSCMLT-202 3 BSCMLT-203 4 BSCMLT-204 5 BSCMLT-205 6 BSCMLT-206

PaperName Microbiology Anatomy and Histotechnology Hematology and Clinical Pathology Biochemistry Computer Application Practical

40 40 40 40 20 180

10 10 10 10 20 60 -

50 50 50 50 40 240

25 25 25 25 25 -

75 75 75 75 75 -

100 100 100 100 100 500

25

100 100

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Practical 600 Total

Scheme of Papers Diploma in Radiology & Imaging Technology (DRIT) Applicable to Septemebr 2005 Admissions onward Sr Semester One Contact Hours (PerSemester) Practical Hrs Theory Hrs Theory Exam Marks Practical Exam Marks Duration of Exams Remarks

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 DRIT-101 2 DRIT-102 3 DRIT-103 4 DRIT-104 5 DRIT-105 6 DRIT-106 7 DRIT-107

PaperName Anatomy and Physiology & Related Pathology Radiation Physics Dark Room Techniques Radiation Hazards & Safety Introductory Biology Communication & Soft Skills Practicals Total

30 40 40 30 20 20 -

10 10 10 10 10 10 -

40 50 50 40 30 30 240

25 25 25 25 25 -

75 75 75 75 75 -

100 100 100 100 100 500

50 25

50 100 150

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Practical 650 Total Duration of Exams

Sr Semester

Two

Contact Hours (PerSemester) Practical Hrs Theory Hrs

Theory Exam Marks

Practical Exam Marks

Remarks

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 DRIT-201 2 DRIT-202 3 DRIT-203 4 DRIT-204 5 DRIT-205

PaperName General Radiology Special Investigation Radiology Electrocardiography & Techniques Advanced Diagnostic Techniques Practicals

40 40 40 40 Total 160

20 20 20 20 80

60 60 60 60 240

25 25 25 25 -

75 75 75 75 -

100 100 100 100 400

25

100 100

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Practical 500 Total

Scheme of Papers Revised BSc Bio-Technology (BSc BT) Applicable to Septemebr 2005 Admissions onward Sr Semester One Contact Hours (PerSemester) Practical Hrs Theory Hrs Theory Exam Marks Practical Exam Marks Duration of Exams Remarks

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 BSCBT-101 2 BSCBT-102 3 BSCBT-103 4 BSCBT-104 5 BSCBT-105 6 BSCBT-106

PaperName Introduction to Biotechnology General Microbiology Biochemistry Introductory Biology Communication & Soft Skills Practical

40 40 40 20 20 Total 160

20 20 20 10 10 80

60 60 60 30 30

25 25 25 25 -

75 75 75 75 -

100 100 100 100 400

50 25

50 100 150

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs

Practical 550 Total

240

Sr Semester

Two

Contact Hours (PerSemester) Practical Hrs Theory Hrs

Theory Exam Marks

Practical Exam Marks

Duration of Exams

Remarks

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 BSCBT-201 2 BSCBT-202 3 BSCBT-203 4 BSCBT-204 5 BSCBT-205

PaperName Genetic Texonomy & Genome Enzymology Immunology Genetic Engineering Practical

40 40 40 40 Total 160

20 20 20 20 80

60 60 60 60 240

25 25 25 25 -

75 75 75 75 -

100 100 100 100 400

25

100 100

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Practical 500 Total Duration of Exams

Sr Semester

Three

Contact Hours (PerSemester) Practical Hrs Theory Hrs

Theory Exam Marks

Practical Exam Marks

Remarks

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 BSCBT-301 2 BSCBT-302 3 BSCBT-303 4 BSCBT-304 5 BSCBT-305

PaperName Fermentation Techniques Cell Biology Tissue Culture Molecular Biology Practical

40 40 40 40 Total 160

20 20 20 20 80

60 60 60 60 240

25 25 25 25 -

75 75 75 75 -

100 100 100 100 400

25

100 100

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Practical 500 Total Duration of Exams

Sr Semester

Four

Contact Hours (PerSemester) Practical Hrs Theory Hrs

Theory Exam Marks

Practical Exam Marks

Remarks

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 BSCBT-401 2 BSCBT-402 3 BSCBT-403 4 BSCBT-404 5 BSCBT-405

PaperName Bio-Chemical Techniques Bio-Physical Techniques Industrial Biotechnology Instrumentation Practical

40 40 40 40 Total 160

20 20 20 20 80

60 60 60 60 240

25 25 25 25 -

75 75 75 75 -

100 100 100 100 400

25

100 100

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Practical 500 Total Duration of Exams

Sr Semester

Five

Contact Hours (PerSemester)

Theory Exam Marks

Practical Exam Marks

Remarks

Practical Hrs

Theory Hrs

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 BSCBT-501 2 BSCBT-502 3 BSCBT-503 4 BSCBT-504 5 BSCBT-505

PaperName Animal Bio-technology Plant Bio-Technology Enviornmental Biotechnology Pharmaceutical Biotechnology Practical

40 40 40 40 Total 160

20 20 20 20 80

60 60 60 60 240

25 25 25 25 -

75 75 75 75 -

100 100 100 100 400

25

100 100

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Practical 500 Total Duration of Exams

Sr Semester

Six

Contact Hours (PerSemester) Practical Hrs Theory Hrs

Theory Exam Marks

Practical Exam Marks

Remarks

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 BSCBT-601 2 BSCBT-602 3 BSCBT-603 4 BSCBT-604 5 BSCBT-605

PaperName Bio-informatics Drug Design Research Methodology Information Technology & Bioinformatics Practical

40 40 40 40 Total 160

20 20 20 20 80

60 60 60 60 240

25 25 25 25 -

75 75 75 75 -

100 100 100 100 400

25

100 100

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Practical 500 Total

Scheme of Papers BSc. Medical Lab Technology (BSCMLT) Applicable to Septemebr 2005 Admissions onward Semester One Contact Hours (PerSemester) Practical Hrs Theory Hrs Theory Exam Marks Practical Exam Marks Duration of Exams Remarks

Total Hrs

External

External 0 75

Internal

Internal

Total

Sr PaperCode 1 BSCMLT-101 2 BSCMLT-102 3 BSCMLT-103 4 BSCMLT-104 5 BSCMLT-105 6 BSCMLT-106 7 BSCMLT-107

PaperName Microbiology Anatomy and Histotechnology Hematology and Clinical Pathology Biochemistry Introductory Biology Communication & Soft Skills Practical Total

40 30 40 30 20 20 180

10 10 10 10 10 10 60

50 40 50 40 30 30 240

25 25 25 25 25 -

75 75 75 75 75 -

100 100 100 100 100 500

50 25

50 100 160

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Practical Practical 650 Total Duration of Exams

Semester

Two

Contact Hours (PerSemester) Practical Hrs Theory Hrs

Theory Exam Marks

Practical Exam Marks

Remarks

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 BSCMLT-201 2 BSCMLT-202 3 BSCMLT-203 4 BSCMLT-204 5 BSCMLT-205 6 BSCMLT-206

PaperName Microbiology Anatomy and Histotechnology Hematology and Clinical Pathology Biochemistry Computer Application Practical

40 40 40 40 20 180

10 10 10 10 20 60 -

50 50 50 50 40 240

25 25 25 25 25 -

75 75 75 75 75 -

100 100 100 100 100 500

25

100 100

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Practical 600 Total Duration of Exams

Semester

Three

Contact Hours (PerSemester) Practical Hrs Theory Hrs

Theory Exam Marks

Practical Exam Marks

Remarks

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 BSCMLT-301 2 BSCMLT-302 3 BSCMLT-303 4 BSCMLT-304 5 BSCMLT-305

PaperName Microbiology Hematology Biochemistry Practical

40 40 40 Total 160

20 20 20 20 80

60 60 60 60 240

25 25 25 25 -

75 75 75 75 -

100 100 100 100 400

25

100 100

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Practical 500 Total Duration of Exams

Basic Cellular Pathology & Allied Technology 40

Semester

Four

Contact Hours (PerSemester) Practical Hrs Theory Hrs

Theory Exam Marks

Practical Exam Marks

Remarks

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 BSCMLT-401 2 BSCMLT-402 3 BSCMLT-403 4 BSCMLT-404 5 BSCMLT-405

PaperName Microbiology Hematology Biochemistry Practical

40 40 40 Total 160

20 20 20 20 80

60 60 60 60 240

25 25 25 25 -

75 75 75 75 -

100 100 100 100 400

25

100 100

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Practical 500 Total

Basic Cellular Pathology & Allied Technology 40

Semester

Five

Contact Hours (PerSemester) Practical Hrs Theory Hrs

Theory Exam Marks

Practical Exam Marks

Duration of Exams

Remarks

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 BSCMLT-501 2 BSCMLT-502 3 BSCMLT-503 4 BSCMLT-504 5 BSCMLT-505

PaperName Microbiology Histopathology Hematology Biochemistry Practical

40 40 40 40 Total 160

20 20 20 20 80

60 60 60 60 240

25 25 25 25 -

75 75 75 75 -

100 100 100 100 400

25

100 100

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Practical 500 Total Duration of Exams

Semester

Six

Contact Hours (PerSemester) Practical Hrs Theory Hrs

Theory Exam Marks

Practical Exam Marks

Remarks

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 BSCMLT-601 2 BSCMLT-602 3 BSCMLT-603 4 BSCMLT-604 5 BSCMLT-605

PaperName Microbiology Histopathology Hematology Biochemistry Practical

40 40 40 40 Total 160

20 20 20 20 80

60 60 60 60 240

25 25 25 25 -

75 75 75 75 -

100 100 100 100 400

25

100 100

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Practical 500 Total

Scheme of Papers BSc Clinical Microbiology (BSc CMB) Applicable to Septemebr 2005 Admissions onward Sr Semester One Contact Hours (PerSemester) Practical Hrs Theory Hrs Theory Exam Marks Practical Exam Marks Duration of Exams Remarks

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 BSCMB-101 2 BSCMB-102 3 BSCMB-103 4 BSCMB-104 5 BSCMB-105 6 BSCMB-106 7 BSCMB-107

PaperName Human Anatomy Human Physiology Clinical Biochemistry Bacterial Morphology & Classification Introductory Biology Communication & Soft Skills Practical

30 30 40 40 20 20 Total 180

10 10 10 10 10 10 60

40 40 50 50 30 30

25 25 25 25 -

75 75 75 75 -

100 100 100 100 400

50 25

50 100 150

Total 3 Hrs 3 Hrs 3 Hrs

Practical 3 Hrs Practical 550 Total Duration of Exams

240

Sr Semester

Two

Contact Hours (PerSemester) Practical Hrs Theory Hrs

Theory Exam Marks

Practical Exam Marks

Remarks

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 BSCCMB-201 2 BSCCMB-202 3 BSCCMB-203 4 BSCCMB-204 5 BSCCMB-205

PaperName General Bacterology General Microbiology Biochemistry Basic Cellular Pathology Practical

40 40 40 40 Total 160

20 20 20 20 80

60 60 60 60 240

25 25 25 25 -

75 75 75 75 -

100 100 100 100 400

25

100 100

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Practical 500 Total Duration of Exams

Sr Semester

Three

Contact Hours (PerSemester) Practical Hrs Theory Hrs

Theory Exam Marks

Practical Exam Marks

Remarks

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 BSCCMB-301 2 BSCCMB-302 3 BSCCMB-303 4 BSCCMB-304 5 BSCCMB-305

PaperName Nutrition & Microbial Metabolism Methodology of Staining Microbial Molecular Genetics Applied Immunology Practical

40 40 40 40 Total 160

20 20 20 20 80

60 60 60 60 240

25 25 25 25 -

75 75 75 75 -

100 100 100 100 400

25

100 100

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Practical 500 Total Duration of Exams

Sr Semester

Four

Contact Hours (PerSemester) Practical Hrs Theory Hrs

Theory Exam Marks

Practical Exam Marks

Remarks

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 BSCCMB-401 2 BSCCMB-402 3 BSCCMB-403 4 BSCCMB-404 5 BSCCMB-405

PaperName Bacterial Pathogenes and Associated Dieses Lab Diagnosis of Microbial Dieses Virology Mycology Practical

40 40 40 40 -

20 20 20 20 80

60 60 60 60 240

25 25 25 25 -

75 75 75 75 -

100 100 100 100 400

25

100 100

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Practical 500 Total

Total 160

Sr Semester

Five

Contact Hours (PerSemester) Practical Hrs Theory Hrs

Theory Exam Marks

Practical Exam Marks

Duration of Exams

Remarks

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 BSCCMB-501 2 BSCCMB-502 3 BSCCMB-503 4 BSCCMB-504 5 BSCCMB-505

PaperName Haematology Paracytology Serology Diagnosis Blood Banking Practical

40 40 40 40 Total 160

20 20 20 20 80

60 60 60 60 240

25 25 25 25 -

75 75 75 75 -

100 100 100 100 400

25

100 100

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Practical 500 Total Duration of Exams

Sr Semester

Six

Contact Hours (PerSemester) Practical Hrs Theory Hrs

Theory Exam Marks

Practical Exam Marks

Remarks

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 BSCCMB-601 2 BSCCMB-602 3 BSCCMB-603 4 BSCCMB-604 5 BSCCMB-605

PaperName Applied Microbiology Automation & Computerisation in Medical Microbiology Preventive Medicine & Health Care Research Methodology Practical

40 40 40 40 Total 160

20 20 20 20 80

60 60 60 60 240

25 25 25 25 -

75 75 75 75 -

100 100 100 100 400

25

100 100

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Practical 500 Total

Scheme of Papers BSc in Bio-Informatics (BSc BI) Applicable to Septemebr 2005 Admissions onward Sr Semester One Contact Hours (PerSemester) Practical Hrs Theory Hrs Theory Exam Marks Practical Exam Marks Duration of Exams Remarks

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 BSCBI-101 2 BSCBI-102 3 BSCBI-103 4 BSCBI-104 5 BSCBI-105 6 BSCBI-106 7 BSCBI-106

PaperName Bioinformatics an Overview General Principles of Chemistry Introduction to Biocomputing Calculus, Function and Number System Introductory Biology Communication & Soft Skills Practical

30 30 40 40 20 20 Total 180

10 10 10 10 10 10 60

40 40 50 50 30 30 240

25 25 25 25 25 -

75 75 75 75 75 -

100 100 100 100 100 500

50 25

50 100 150

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Practical 650 Total Duration of Exams

Sr Semester

Two

Contact Hours (PerSemester) Practical Hrs Theory Hrs

Theory Exam Marks

Practical Exam Marks

Remarks

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 BSCBI-201 2 BSCBI-202 3 BSCBI-203 4 BSCBI-204 5 BSCBI-205

PaperName Biostatistics Organization and Functions of Cells and Organisms Fundamental and Application of Chemistry Biochemistry Practical

40 40 40 40 Total 160

20 20 20 20 80

60 60 60 60 240

25 25 25 25 -

75 75 75 75 -

100 100 100 100 400

25

100 100

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Practical 500 Total Duration of Exams

Sr Semester

Three

Contact Hours (PerSemester) Practical Hrs Theory Hrs

Theory Exam Marks

Practical Exam Marks

Remarks

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 BSCBI-301 2 BSCBI-302 3 BSCBI-303 4 BSCBI-304 5 BSCBI-305

PaperName Human Biology and Evaluation Discrete & Numerical Mathematics Metabolism Calculas, Vector Calculus & Linear Algebra Practical

40 40 40 40 Total 160

20 20 20 20 80

60 60 60 60 240

25 25 25 25 -

75 75 75 75 -

100 100 100 100 400

25

100 100

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Practical 500 Total Duration of Exams

Sr Semester

Four

Contact Hours (PerSemester) Practical Hrs Theory Hrs

Theory Exam Marks

Practical Exam Marks

Remarks

Total Hrs

External

External -

Internal

Internal

Total

Sr PaperCode 1 BSCBI-401

PaperName Molecular Genetics

40

20

60

25

75

100

-

-

Total 3 Hrs

2 BSCBI-402 3 BSCBI-403 4 BSCBI-404 5 BSCBI-405

Object Oriented Programming Using C++ Management of Biological Databases Artificial Intelligence Practical

40 40 40 -

20 20 20 80

60 60 60 240

25 25 25 -

75 75 75 -

100 100 100 400

25

75

100 100

3 Hrs 3 Hrs 3 Hrs 3 Hrs Practical 500 Total

Total 160

Sr Semester

Five

Contact Hours (PerSemester) Practical Hrs Theory Hrs

Theory Exam Marks

Practical Exam Marks

Duration of Exams

Remarks

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 BSCBI-501 2 BSCBI-502 3 BSCBI-503 4 BSCBI-504 5 BSCBI-505

PaperName Immunology Biomarkup Language and System Programming Programming Project Partial Differential Equation Practical

40 40 40 40 Total 160

20 20 20 20 80

60 60 60 60 240

25 25 25 25 -

75 75 75 75 -

100 100 100 100 400

25

100 100

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Practical 500 Total Duration of Exams

Sr Semester

Six

Contact Hours (PerSemester) Practical Hrs Theory Hrs

Theory Exam Marks

Practical Exam Marks

Remarks

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 BSCBI-601 2 BSCBI-602 3 BSCBI-603 4 BSCBI-604 5 BSCBI-605

PaperName Microbiology & Virology Numerical Analysis Algorithms, Data Structures and Compilers Software Engineering Practical

40 40 40 40 Total 160

20 20 20 20 80

60 60 60 60 240

25 25 25 25 -

75 75 75 75 -

100 100 100 100 400

25

100 100

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Practical 500 Total

Scheme of Papers Post Graduate Diploma in Medical LabTechnology - PGDMLT Applicable to Septemebr 2005 Admissions onward Semester One Contact Hours (PerSemester) Practical Hrs Theory Hrs Theory Exam Marks Practical Exam Marks Duration of Exams Remarks

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 MSCMLT-101 2 MSCMLT-102 3 MSCMLT-103 4 MSCMLT-104 5 MSCMLT-105 6 MSCMLT-106 7 MSCMLT-107

PaperName Human Anatomy & Physiology Basic Biochemistry Clinical Biochemistry Basic Clinical Pathology Introductory Biology Communication & Soft Skills Practical

40 30 30 40 20 20 Total 180

10 10 10 10 10 10 60

50 40 40 50 30 30 240

25 25 25 25 25 -

75 75 75 75 75 -

100 100 100 100 100 500

50 25

50 100 150

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Practical 3 Hrs Practical 650 Marks Duration of Exams

Semester

Two

Contact Hours (PerSemester) Practical Hrs Theory Hrs

Theory Exam Marks

Practical Exam Marks

Remarks

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 MSCMLT-201 2 MSCMLT-202 3 MSCMLT-203 4 MSCMLT-204 5 MSCMLT-205

PaperName Instrumentation Clinical Haematology Blood Banking Lab Management Practical

40 40 40 40 Total 160

20 20 20 20 80 -

60 60 60 60 240

25 25 25 25 -

75 75 75 75 -

100 100 100 100 400

25

100 100

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Practical 500 Marks

Scheme of Papers M.Sc. (BioTechnology) - MSCBT Applicable to Septemebr 2005 Admissions onward Semester One Contact Hours (PerSemester) Practical Hrs Theory Hrs Theory Exam Marks Practical Exam Marks Duration of Exams Remarks

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 MSCBT-101 2 MSCBT-102 3 MSCBT-103 4 MSCBT-104 5 MSCBT-105 6 MSCBT-106 7 MSCBT-107

PaperName Introductory Biology Molecular Biology-1 Bioenergetics & Metabolism Microbiology Biophysical Chemistry & Instrumentation Communication & Soft Skills Practical

20 40 30 40 30 20 Total 180

10 10 10 10 10 10 60

30 50 40 50 40 30 240

25 25 25 25 25 -

75 75 75 75 75 -

100 100 100 100 100 500

50 25

50 100 150

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Practical 650 Marks Duration of Exams

Semester

Two

Contact Hours (PerSemester) Practical Hrs Theory Hrs

Theory Exam Marks

Practical Exam Marks

Remarks

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 MSCBT-201 2 MSCBT-202 3 MSCBT-203 4 MSCBT-204 5 MSCBT-205

PaperName Enzymology & Enzyme Technology Bioinformatics Molecular Biology-II Genetic Engineering Practical

40 40 40 40 Total 160

20 20 20 20 80 -

60 60 60 60 240

25 25 25 25 -

75 75 75 75 -

100 100 100 100 400

25

100 100

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Practical 500 Marks Duration of Exams

Semester

Three

Contact Hours (PerSemester) Practical Hrs Theory Hrs

Theory Exam Marks

Practical Exam Marks

Remarks

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 MSCBT-301 2 MSCBT-302 3 MSCBT-303 4 MSCBT-304 5 MSCBT-305

PaperName Immunology Plant Bio-Technology Enviornmental Biotehnology Bioprocess Engineering & Technology Practical

40 40 40 40 Total 160

20 20 20 20 80

60 60 60 60 240

25 25 25 25 -

75 75 75 75 -

100 100 100 100 400

25

100 100

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Practical 500 Marks Duration of Exams

Semester

Four

Contact Hours (PerSemester) Practical Hrs Theory Hrs

Theory Exam Marks

Practical Exam Marks

Remarks

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 MSCBT-401 2 MSCBT-402 3 MSCBT-403 4 MSCBT-404 5 MSCBT-405

PaperName Biostatistics Animal Biotechology Pharmaceutical Biotechnology Research Methodology Practical / Training

40 40 40 40 Total 160

10 10 10 10 40

50 50 50 50 200

25 25 25 25 -

75 75 75 75 -

100 100 100 100 400

25

100 100

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Practical 500 Marks

Page 1 of 1

Scheme of Papers M.Sc. ( Clinical Microbiology ) - MSCCMB Applicable to Septemebr 2005 Admissions onward Semester One Contact Hours (PerSemester) Practical Hrs Theory Hrs Theory Exam Marks Practical Exam Marks Duration of Exams Remarks

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 MSCCMB-101 2 MSCCMB-102 3 MSCCMB-103 4 MSCCMB-104 5 MSCCMB-105 6 MSCCMB-106 7 MSCCMB-107

PaperName Human Anatomy Human Physiology Clinical Biochemistry Histopathology Introductory Biology Communication & Soft Skills Practical

40 30 30 40 20 20 Total 180

10 10 10 10 10 10 60

50 40 40 50 30 30 240

25 25 25 25 25 -

75 75 75 75 75 -

100 100 100 100 100 500

50 25

50 100 150

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Practical 3 Hrs Practical 650 Marks Duration of Exams

Semester

Two

Contact Hours (PerSemester) Practical Hrs Theory Hrs

Theory Exam Marks

Practical Exam Marks

Remarks

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 MSCCMB-201 2 MSCCMB-202 3 MSCCMB-203 4 MSCCMB-204 5 MSCCMB-205

PaperName General Bacteriology Immunology Haematology Blood Banking Practical

40 40 40 40 Total 160

20 20 20 20 80 -

60 60 60 60 240

25 25 25 25 -

75 75 75 75 -

100 100 100 100 400

25

100 100

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Practical 500 Marks Duration of Exams

Semester

Three

Contact Hours (PerSemester) Practical Hrs Theory Hrs

Theory Exam Marks

Practical Exam Marks

Remarks

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 MSCCMB-301 2 MSCCMB-302 3 MSCCMB-303 4 MSCCMB-304 5 MSCCMB-305

PaperName Systematic Bacteriology Applied Microbiology Basic Cellular Pathology Molecuar Biology Practical

40 40 40 40 Total 160

20 20 20 20 80

60 60 60 60 240

25 25 25 25 -

75 75 75 75 -

100 100 100 100 400

25

100 100

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Practical 500 Marks Duration of Exams

Semester

Four

Contact Hours (PerSemester) Practical Hrs Theory Hrs

Theory Exam Marks

Practical Exam Marks

Remarks

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 MSCCMB-401 2 MSCCMB-402 3 MSCCMB-403 4 MSCCMB-404 5 MSCCMB-405

PaperName Virology Mycology Parasitology Research Methodology & Techniques Practical / Training

40 40 40 40 Total 160

10 10 10 10 40

50 50 50 50 200

25 25 25 25 -

75 75 75 75 -

100 100 100 100 400

25

100 100

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Practical 500 Marks

Scheme of Papers MSc in Bio-Informatics (MSc BI) Applicable from September 2005 Semester onwards Sr Semester One Contact Hours (PerSemester) Practical Hrs Theory Hrs Theory Exam Marks Practical Exam Marks Duration of Exams Remarks

Total Hrs

External

External 75 75

Internal

Internal

Total

Sr PaperCode 1 MSCBI-101 2 MSCBI-102 3 MSCBI-103 4 MSCBI-104 5 MSCBI-105 6 MSCBI-106

PaperName Bioinformatics - 1 Biochemistry - 1 Machine Learning Theory of Statistics and Genetic Statistics Communication & Soft Skills Practicals

25 25 25 25 -

75 75 75 75 -

100 100 100 100 -

25 25

100 100

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Duration of Exams

Sr Semester

Two

Contact Hours (PerSemester) Practical Hrs Theory Hrs

Theory Exam Marks

Practical Exam Marks

Remarks

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 MSCBI-201 2 MSCBI-202 3 MSCBI-203 4 MSCBI-204 5 MSCBI-205

PaperName Bioinformatics - 2 Biochemistry - 2 Design and Analysis of Algorithms Database Systems Practicals

25 25 25 25 Contact Hours (PerSemester) Practical Hrs Theory Hrs

75 75 75 75 -

100 100 100 100 -

25

100

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Duration of Exams

Sr Semester

Three

Theory Exam Marks

Practical Exam Marks

Remarks

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 MSCBI-301 2 MSCBI-302 3 MSCBI-303 4 MSCBI-304 5 MSCBI-305

PaperName Introduction to Datastructures of Biologists Biocomputing and Computational Biology Software Engineering Pharmacology Practicals

25 25 25 25 Contact Hours (PerSemester) Practical Hrs Theory Hrs

75 75 75 75 -

100 100 100 100 -

25

100

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs Duration of Exams

Sr Semester

Four

Theory Exam Marks

Practical Exam Marks

Remarks

Total Hrs

External

External 75

Internal

Internal

Total

Sr PaperCode 1 MSCBI-401 2 MSCBI-402 3 MSCBI-403 4 MSCBI-404 5 MSCBI-405

PaperName Introduction to Java and Introductory Combinatorics Basic Research Skills Computational Techniques for Genome Assembly and Analysis Preoteomics Practicals

25 25 25 25 -

75 75 75 75 -

100 100 100 100 -

25

100

Total 3 Hrs 3 Hrs 3 Hrs 3 Hrs 3 Hrs

Page 1 of 1

PUNJAB TECHNICAL UNIVERSITY Jalandhar

DISTANCE EDUCTION PROGRAMME http://www.ptu.ac.in

Syllabus

September 2005

© Copyright, Punjab Technical University

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

Diploma in Operation Theatre Technology

SEMESTER I

DOTT-101: Anatomy and Physiology

Anatomy 1. Introduction to Anatomy & Histology, Structure of cell, epithelial tissue, muscular tissue, nervous tissue. 2. Skeletal System, Structure of bones, types of bones, Bones of cranium, face vertebral column upper and lower limbs, fracture of bones, various movements of joints. 3. Muscular System, Structure and types of muscles in human body, important muscles and their group action. 4. Circulation System, Structure of heart, names and position of main blood vessels. 5. Lymphatic System, Lymph vessels, lymph nodes and lymphoid organs, their structure & functions. 6. Digestive systems. Parts of gastrointestinal tract and associated glands. 7. Respiratory System. Parts of Respiratory System. 8. Urinary System. Parts of Urinary System. 9. Endocrine System. Various endocrine glands. Thyroid. Parathyroid. Adrenal glands pituitary pancreas. Thymus and sex glands. 10. Reproductive System. Male & female Reproductive organs. 11. Skin and sense organs. Eye, Ear, Nose. Taste Buds 12. Nervous System. Parts of brain, spinal cord, peripheral nerves. Physiology 1 Blood. Composition and function of blood, haemopesis, blood coagulation. Blood groups, body fluid. 2 Cardiovascular Systems. Circulation of blood. function of heart and blood vessels. Control of heart rate, pulse, regulation of blood pressure, blood volume. 3 Respiratory system. Function of lungs, mechanism of breathing and exchange of gases in the lungs, regulation of respiration, Respiration disorder like anoxia.dyspnea cyanosis etc.artificial respiration lung function tests. 4 Digestive Systems. Digestion of food in mouth, stomach & small intestines. Absorption offood, function of liver function tests. 5 Excretory Systems. Structure & function of kidney and urinary bladder. Mechanism of urine formation. disorders of kidney. 6 Endocrine Glands Functions of various endocrine glands and hormones secreted by them. 7 Reproductive Systems. Physiology & female reproductive organs. 8 Nervousd System.Neurone & its function, function of central nervous system, Autonomies nervous system , physiology of vision, hearing & olfaction. PRACTICAL: Identification of bones & other organs of the body and viva voce as per theory syllabus.

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SEMESTER I

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

DOTT-102: Pathology & Microbiology

Pathology CHAPTER 1: CELL INJURY AND ADAPTATION: Causes of cell injury. Mechanism of cellinjury,ishaemic & hypoxic injury, chemical injury ,reversible injury,nercosis ,cellular adaptation of growth & differentiation atrophy,hypertrophy,metaplasia,hyperplasia,classification of tumors ,premalignant lesion,diagniosis of cancer. CHAPTER 2: ACUTE AND CHRONIC INFLAMATION Acute vascular changes.phagocytosts Chemical mediators of inflammation. Definition and causes of chronic inflammation. granulematous inflammation, system manifestations of inflammation CHAPTER 3: DISORDERS OF VASCULAR FLOW AND SHOCK: Oedema, hyperemia or congestion, thrombosis, embolism. Infarction shock, Ischemia, Over hydration, dehydration. CHAPTER 4: THE RESPONSE TO INFECTION Categories of infectious agents, host barriers to infection, how disease is caused, inflammatory response to infectious agents CHAPTER 5: THE HAEMATOPOHTIC AND IYMPHOID SYSTEM: Haemorrhage.various type of Anaemia,leakopenia,leakoeytosis,bleeding disorders coagulation mechanism ,maintenance of blood volume. Abnormalities of ph of blood. Microbiology CHAPTER 1: INTRODUCTION TO MICROBIOLOGY: Discovery of micro organisms. Classification and general characteristics of microorganisms, nutritional requirements of Micro-organisms. CHAPTER 2: PRINCIPLES OF MICROBIAI CONTROL: Sterilization, importance of sterilization in OT techniques various methods-physical UV radiation, alcohols and heavy metals, Autoclave structure functioning control and indicators. CHAPTER 3: IMMUNOLOGY: Antigen-antibody reaction anaphylaxis hypersensitivity CHAPTER 4: VIROLOGY Imp disease caused by different viruses of mode of infection SEMESTER I

DOTT-103: Surgical Equipment and Machinery

CHAPTER 1: Armamentarium: Cox and storing in O.T, Sterlization and disinfections CHAPTER 2: GENERAL SURGICAL PRINCIPLES AND INSTRUMENTS The surgical patient, operation room technique . CHAPTER 3 : INSTRUMENTS USED FOR PREPAIRING SURGICAL Cheatles forceps,rampely,s sponge holding forceps mayo's towel chip,esmach's bandage, Simple tourniquet, pneumatic tourniquet CHAPTER 4 : INCISION MAKING METHOD AND INSTRUMENTS : Bard parker knife handle, major abdominal incision, artery forceps and their types instruments used in homeostasis, Kocher's forceps, electric cautery. CHAPTER 5:

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RETRACTORS: Single hook retractors , Czerny's retractor, s, nerve hook retractors, Morris retractors, deaver's, retractors. CHAPTER 6 : WOUND MANAGEMENT Seissors and its types sucking material and techniques,disinfectants and irritants, dressing procedures ,different types of bandages, surgical needle & needle holders, various types of suture material PRACTICAL : Identification & Demonstration of working of the equipment and viva voce as per theory syllabus.

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

SEMESTER I

DOTT-104: Anesthesia Equipment & Drugs

Anesthesia Equipment 1 Boyle's Machine & it's functioning 2 Boyle's vaporizer 3 Magill's breathing circuit, Bains breathing circuit, pediatrics anesthesia circuit 4 Gas cylinders and flow meters 5 Carbon dioxide absorption contester 6 Suction apparatus-foot operated, electrically operated 7 Ambubag laryngoscope hndotracheatubes 8 Catheters,face masks, venti mask Drugs 1 General Principles ­ Pharmacological classification of drugs. Route of drug administration, precautions in administration, principles of drug toxicity, prevention and treatment of poisoning adverse drug reaction 2 Sedatives & Hypnoties-Barbutrates morphine and others 3 Important groups of drugs, N Saids, ibuprofen, aspirin, antimicrobial agents anti allergy drugs , anti diuretics 4 Pre-anesthetic mediation 5 Local Anesthetic agents 6 Spinal Anesthetic agents 7 General Anesthetic agents PRACTICAL: Identification & demonstration of the working of equipments & viva voce as per theory syllabus

Paramedical Syllabus Manual : 3 / 144

SEMESTER I

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

DOTT-105: Communication & Soft Skills

UNIT I Essentials of Grammar: · · · · UNIT II Office Management: · · · · · · · UNIT III Letter & Resume Writing: · · · · · · · UNIT IV Presentation Skills: · · · · · · · · · · · UNIT V Interview Preparation: · · · · · · · Types of Interview Preparing for the Interviews Attending the Interview Interview Process Employers Expectations General Etiquette Dressing Sense

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Parts of Speech Punctuation Vocabulary Building Phonetics

Types of Correspondence Receipt and Dispatch of Mail Filing Systems Classification of Mail. Role & Function of Correspondence MIS Managing Computer

Types of Letters-Formal / Informal Importance and Function Drafting the Applications Elements of Structure Preparing the Resume Do's & Don'ts of Resume Helpful Hints

Importance of Presentation Skills Capturing Data Voice & Picture Integration Guidelines to make Presentation Interesting Body Language Voice Modulation Audience Awareness Presentation Plan Visual Aids Forms of Layout Styles of Presentation.

· UNIT VI

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

Postures & Gestures

Group Discussion & Presentation: · · · · · Definition Process Guidelines Helpful Expressions Evaluation

(Note: Every student shall be given 15 minutes. of presentation time & 45 minutes of discussion on his/ her presentation.) The student will be evaluated on the basis of : his / her presentation style Feedback of Faculty & Students General Etiquette Proficiency in Letter Drafting / Interview Preparation The paper is internal and at least 3 tests will be taken. Best 2 of 3 shall account for final grades (70% Test & 30% Presentation)

Paramedical Syllabus Manual : 5 / 144

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

SEMESTER I

DOTT-106: Introductory Biology

Unit I Living World Biology & Its Branches; relationships with other sciences; scientific methods in Biology; historical breakthroughs; scope of biology and career options; role of Biology in dispelling myths and misbelieves; characters of living organisms, (elementary idea of metabolism, transfer of energy at molecular level, open and closed systems, omoeostasis, growth and reproduction, adaptation, survival, death). Origin and evolution of life - theories of evolution; evidence of evolution; sources of variations ( mutation, recombination, genetic drift, migration, natural selection); concept of species; specification and isolation (geographical and reproductive); origin of species. Unit II Diversity of Life Variety of living organisms, Systematic; need, history and types of classification (artificial, natural, polygenetic); biosystematics; binomial nomenclature; Two kingdom system, Five kingdom System, their merits and demerits, status of bacteria and virus; botanical gardens and herbia; zoological parks and museums. Unit III Cell and Cell Division Cell as a basic unit of life - discovery of cell, cell theory, cell as a self - contained unit; procaryotic and eukaryotic cell; unicellular and multicellular organisms; tools and techniques ( compound microscope, electron microscope and cell fractionation); Ultrastructure of prokaryoytic and eukaryotic cell - cell wall, cell membrane - unit membrane concept (flauid mosaic model); membrane transport; cellular movement (exocytosis, endocytosis); cell organelles and their functions - nucleus, mitochondria, plastids, endoplamasic reticulum, Gogli complex, lysosomes, lysosomes, microtubules, centriole, vacuole, cytoskeleton, cilia and flagella, ribosomes. Molecules of cell; inorganic and organic materials - water, salt, mineral ions, carbohydrates, lipids, amino acids, proteins, nucleotides, nycleic acids (DNA and RNA); Enzymes (Properties, chemical nature and mechanism of action); vitamins, hormones and steroids. Unit IV Genetics Continuity of life - heredity, variation; mendel's laws of inheritance, chromosomal basis of inheritance; other patterns of inheritance - incomplete dominance, multiple allelism, quantitative inheritance. Chromosomes - bacterial cell and eukaryotic cell; parallelism between genes and chromosomes; genome, linkage and crossing over; gene mapping; recombination; sex chromosomes; sex determination; sex linked inheritance; mutation and chromosomal aberrations; Human genetics - methods of study, genetic disorders. DNA as a genetic material - its structure and repliaction; structure of RNA and its role in protein synthesis; Gene expression - transcription and translation in prokaryotes and eukaryotes; regulation of gene expression, induction and repression - housekeeping genes; nuclear basis of differentiation and development; oncoenes. Basics of Recombinant DNA technolgy; cloning; gene bank; DNA fingerprinting; genomics principles and applications, transgenic plants, animals and microbes. Unit V Morphology of Plants and Animals

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Morphology - root, stem and leaf, their sturucture and modification; Inflorescence, flower, fruit, seed and their types;Description of Poaceae, Liliaceae, Fabaceae, Solanaceae, Brassicaceae and Asteraceae. Morphology of animals - salient features of earthworm, cockroach and rat; tissue systems, structure and function of tissues - epithelial, connective, muscular and nervious. Practical 1. Study of parts of Compound Microsope 2. Study of mitosis in onion root tip and animal cell (grasshopper) 3. Study of meiosis in onion flower buds, and testis iof grasshopper. 4. Study of cyclosis in leaf cell of Hydrilla, or Tradescantia and in Paramoecium. 5. Study of cell wall components ( cellulose, lignin, suberin and mucilage). 6. Study of mitochondria by staining with a Janus Green.

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

7. Study of specimens and their identification with reason - Bacteria, Oscillator, Spirogyra, Rhizopus, mushroom/braket fungi, yeast, liverwort, moss, fern, Pinus, one monocotyledon, one dicotyledon and lichens. 8. Study of characters of specimens and identification with reason - Amoeba, Hydra, Liver - Fluke, Ascaris, Leech, Earthworm, Prwen, Silk moth honey bee, snail, Starfish, Dogfish, Rohu, Frog, Lizards, Pigeon/ any other bird and rabbit/ any other mammal. 9. Study of squamous epithelium, muscle fibres, nerve cells and mammalian blood film through temporary/permanent slides. 10. Study of external morphology of earthworm, cocroach, from and rat through models.

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PTU/DEP/Paramedical/September 2005/Detailed Syllabus

SEMESTER I

DOTT-107 Practical

Anatomy & Physiology: Pathology & Microbiology: Surgical Equipments & Machinery: Anesthesia equipments and Drugs: Introductory Biology: Internal Assessment : 25 Marks 15 Marks 15 Marks 15 Marks 15 Marks 15 Marks

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PTU/DEP/Paramedical/September 2005/Detailed Syllabus

SEMESTER II

DOTT-201: Medicine

CHAPTER 1: HISTORY TAKING AND SYMPTOMALOGY OF VARIOUS DISEASES.FEVER, polyuria, heart, burns, vomiting ,diarrhea, jaundice, epistaxis, Chapter 2: ABDOMEN Hepatomegaly, spleenomegaly, ascitis, cirrhosis, hepatitis, malaria, typhoid, aids. CHAPTER 3 DISORDERS OF CICULATORY AND RESPIRATORY SYSTEM Pleural effusion, pulmonary tuberculosis, pneumonia, dyspnoea, asphyxia, hypoxia, cardiac failure, pericardial effusion, congential heart diseases, cyanosis, hypertension, cardiac monitors, defibrillator. CHAPTER 4 DISORDER OF NERVOUS AND ENDOCRINE SYSTEM: Hemiplagia,periplagia,paralysis,Parkinson,s disease ,coma,diabetes,Addison,s disease, hyperthyroidism. CHAPTER 5 MEDICAL EMERGENCIES : Cardiac arrest, bronchial asthama,respiratory failure, acute gastroenteritis,meningitis,renal colic,hypoglycemia,acuter poisoning. CHAPTER 6 PREVENTIVE ASPECTS OF MEDICINE: Concept of health, preventive medicine, social Medicine, public health, epidemiology of infectitiuous diseases, methods of prevention and control of infestations diseases. PRACTICAL: Demonstrating the use of medical equipment and viva voce as per theory syllabus.

SEMESTER II

DOTT-202: SURGERY

CHAPTER 1 Introduction of surgery and basic principles of surgery ,basic principles of surgery. CHAPTER 2 : Tumors, benign and malignant ,cysts, ulcers, sinuses, fistula , differential diagnosis of cyst and tumor. CHAPTER 3 : FRACTURES AND DISLOCATION ­ Classification of fracture, management, fixation, reduction, Immobilization, principles of closed reduction, artificial prosthesis. CHAPTER 4 COMPARATIVE AND SURGICAL ANATOMYInvestigation of breast, benign disease, carcinoma of breasts, treatment of carcinoma of breast, mastectomy. CHAPTER 5 : - HEAD INJURY ­Common manifestation, management of patient, surgical interventions. CHAPTER 6 : Cleft lip & palatc,acute appendicitis, urethral strictures. CHAPTER 7 : DIFFERENT SURGICAL INSTRUMENTS: Instruments used in major surgical operation including Biliary ,Tract Surgery, anorectal surgery ,urological surgery, ovthopcedic surgery instruments, plastic surgery instruments PRACTICAL : Demonstrating the use of medical equipment and viva vocae as per theory syllabus.

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PTU/DEP/Paramedical/September 2005/Detailed Syllabus

SEMESTER II

DOTT-203: OBOTETRICS & GYNERACOLOGY

OBOTETERICS : Pregnancy, normal delivery, Forceps Delivery, episiotomy, caesarian delivery, Twin pregnancy birth control methods, Medical termination of pregnancy. GYNAECOLOGY : Anatomy of female sex organs ,gynecological examination and diagonosis, diseases of vulva, diseases of vagina, S.T.D in female, disorders of menstruation. Prolapsed uterus, Fibromyomas of uterus, endometriosis, various ovarian tumors, gynae examination instruments speculum & dialators, instruments of common gynecological and obstetrics procedures or surgery. PRACTICAL : Identification of Instrument and viva voce as per theory syllabus.

DOTT-204: EYE & E.N.T.

EYE CHAPTER 1: INSTURMENTS USED FOR EXAMINATION OF EYE, DESMASEL,S EYE LID : retractor, ophthalmic loop, ophthalmoscope and anatomy of eye. CHAPTER 2 : Clinical methods in ophthalmology ,history taking ,ocular examination & diagnostic test, snell's formulae, tonometry, direct opthoalmoscopy. CHAPTER 3 : Various eye diseases ,diseases of conjunctiva, trachoma, diseases of cornea, ulcerative keratitis, scleritis, diseases of lens, cataract glaucoma, retinal detachment, intraocular lens implementation for cataract. ENT CHAPTER 1: EAR: Anatomy of the ear, diseases of middle ear, Ramasay hunt syndrome, ear of mastoid surgery, parotid surgery & trauma to face, tumors of external car, auricle, middle ear, and rehabilitation of hearing impaired. CHAPTER 2 : NOSE: Anatomy of nose, nasal septum & its diseases ,acute & chronic rhinits, nasal polyps,epistaxis,fracture of nasal septum, CHAPTER 3 : THROAT: Anatomy of throat, diseases of larynx, tracheotomy:- types, steps of operation, post operative care. CHAPTER 4 : Instruments used in operation in Ear, Nose & Throat. PRACTICAL : Demonstration the use of medical equipment and viva voce as per theory syllabus. SEMESTER II

SEMESTER II

DOTT-205: PRACTICAL

Medicine Surgery Obtetrics & Gyneacology EYE & ENT Internal Assessment: 25 Marks

: 15 Marks : 30 Marks : 15 Marks : 15 Marks

Paramedical Syllabus Manual : 10 / 144

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

Diploma in Radiology & Imaging Technology

SEMESTER I

Theory Introduction to Radiology & Radiology Services. Structure of the Body-cell-tissues. Musculo-Skeletal System · Skull-cerebral columns-shoulder girdle, Bones of upper extremities ­ Bones of Lower extremities, gornit muscles, pelvis girdle, thoracic Cage & Bony joints. Cardiovascular System · Heart, Blood, Arteries, Veins Lymphatic System · Circulation of Lymph, Lymph glands, Thoric duct. Digestive System · Mouth - oesophagus ­ stomach ­ small intestine ­ large intestine ­ spleen, liver Gall Bladder ­ Pancreas. Respiratory System · Nose & Larynx, Trachea ­ Lungs Nervous System: · Brain ­ Meanings ­ Ventricles ­ Spinal cord and nerves Reproductive System: · Female & Male Organs Urinary System · Kidney ­ Uterus ­ Bladders, Prostate Skin · Structure and its function gland Supra-renal glands Endocrine System · Pituitary glands ­ Pennel gland ­ Thymus gland ­ Thyroid and parathyroid Eye · Ear · Structure and its functions, surface anatomy Practical: · · · · · · Introduction of the various parts and structure in human body on charts and models. Identification of Bones and skeleton Surface making of human body Identification of bones & parts on X ray films Visit to pathology museum for identification common pathology lesions Visit to antomy museum for identification of various parts of the human body. Structure and its function

DRIT-101: Anatomy and Physiology & Related Pathology

Paramedical Syllabus Manual : 11 / 144

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

SEMESTER I

Theory

DRIT-102: General and Radiation Physics

Heat and methods of transference of heat, Condensers, Inductance of Impendence. A.C. D.C. current, RMS value, peak value Electromagnetic Induction ­ laws , fields, influence Transformer- Principal Construction and Losses of Step Down and High Tension Transformers Diode valves and its use as rectifiers, solid- state rectifiers, its various rectifying circuits using X- ray machines. Production of x-rays and its properties, X-ray tube- stationery anode anode and rotating anode & therapy tubes. X-ray circuit, interlocking circuits, relays and timers Various units used for measuring radiation roentgen, rad and rem. Ionisation chambers, G.M. counter and scintillation counter, interaction of x-rays with matter. (photo electric, comptron and pair production) Quality and qualitity of X-rays, HVT, linear absorption, coeffienct, Gird cones, filters, cylindersm L.B.D. F.F>D, focal spot size etc. Inverse square law, scattered radiations and appliances used to reduce it. Radioactivity: curie, half life, decay factor, Details about radium, cablt caesium, doses- dose and dose rate, exposure dose, exit dose surface dose, depth dose, isodoes charts and their uses. Raditiation hazards protection against it, film badge pocket ionization chamber, maximum permissible does. Gama X-ray film, X-ray tube caliberation, solrization, sensitometer and densitometer, radiation protection devices- Lead shield, lead chair, lead apron, lead goggles, Thyroid and gonad shield, lead gloves etc. Practical · · · · · · · · · Verification of inverse square law Calibration of x-rays machines To check the lead apron for any crack Find out whether the glass in the screen is lead glass or ordinary glass study the characteristic curve of a diode value To survey the X-ray control for radiation Demonstrate that the intensifying effect of X-ray intensifying screen is due to light produced by fluorescent and not due to the x-ray Demonstrate the use of Gird/ potter-bucky diaphragm and radiographic contrast Verification of optical and radiation field coincidence

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PTU/DEP/Paramedical/September 2005/Detailed Syllabus

SEMESTER I

DRIT-103: Dark Room Techniques

Theory Photographic Process · Light image- image produce by radiation- light sensitive material ­ Latent image formation Film materials · The structure of X-ray films resolving power, orthochromatic and panchromatic filsm grains of film- sensitivity of film contrast of films X-ray Films Storage · Storage of unexposed films and protection to exposed films Screens · Construction of intensifying screen-choice of fluorescent materials ­intensifying factors details ­ sharpness-speed, screen contract-care of intensifying screen & rate earh screens. Cassettes · Cassettes designs- care of cassette- mounting of intensifying screen in the cassette. Various types of cassettes, definition, structure of cassettes Factors affecting the developer · Types of developer and fixer- factors affecting the use of fixer. · Silver recovery methods. Components of PQ & MQ developer and fixers, replenisher etc. Film rising and washing and drying, Intermediate rinse- washing and drying Film processing and equipment · Manual and automatic processing method Dark Room Design · Outlay and material used. Entrance, Safelight Ventilation, Construction of wall dry bench & wet bench etc. The Radiographic Image · The sharpness, contrast, details definition, viewing conditions. Miscellaneous · Trimming, identification of films legends-records filling- report distribution Film Artefacts · Photographic and radiation artifacts. Factors affecting the quality control of a radiograph Practical Dark room: · · · · · · · · · How the dark room light be tested for safety How intensifying screen be tested for uniform contact? How intensifying screen be tested for uniform contact? To prepare the developer and fixer Load unload and processing of X-rays film. Overdeveloped film reduction. Screen after glow test Manual and digital subtraction techniques Formation of duplicate and negative radiograph.

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PTU/DEP/Paramedical/September 2005/Detailed Syllabus

SEMESTER I

DRIT-104: Introductory Biology

Unit I Living World Biology & Its Branches; relationships with other sciences; scientific methods in Biology; historical breakthroughs; scope of biology and career options; role of Biology in dispelling myths and misbelieves; characters of living organisms, (elementary idea of metabolism, transfer of energy at molecular level, open and closed systems, omoeostasis, growth and reproduction, adaptation, survival, death). Origin and evolution of life - theories of evolution; evidence of evolution; sources of variations ( mutation, recombination, genetic drift, migration, natural selection); concept of species; specification and isolation (geographical and reproductive); origin of species. Unit II Diversity of Life Variety of living organisms, Systematic; need, history and types of classification (artificial, natural, polygenetic); biosystematics; binomial nomenclature; Two kingdom system, Five kingdom System, their merits and demerits, status of bacteria and virus; botanical gardens and herbia; zoological parks and museums. Unit III Cell and Cell Division Cell as a basic unit of life - discovery of cell, cell theory, cell as a self - contained unit; procaryotic and eukaryotic cell; unicellular and multicellular organisms; tools and techniques ( compound microscope, electron microscope and cell fractionation); Ultrastructure of prokaryoytic and eukaryotic cell - cell wall, cell membrane - unit membrane concept (flauid mosaic model); membrane transport; cellular movement (exocytosis, endocytosis); cell organelles and their functions - nucleus, mitochondria, plastids, endoplamasic reticulum, Gogli complex, lysosomes, lysosomes, microtubules, centriole, vacuole, cytoskeleton, cilia and flagella, ribosomes. Molecules of cell; inorganic and organic materials - water, salt, mineral ions, carbohydrates, lipids, amino acids, proteins, nucleotides, nycleic acids (DNA and RNA); Enzymes (Properties, chemical nature and mechanism of action); vitamins, hormones and steroids. Unit IV Genetics Continuity of life - heredity, variation; mendel's laws of inheritance, chromosomal basis of inheritance; other patterns of inheritance - incomplete dominance, multiple allelism, quantitative inheritance. Chromosomes - bacterial cell and eukaryotic cell; parallelism between genes and chromosomes; genome, linkage and crossing over; gene mapping; recombination; sex chromosomes; sex determination; sex linked inheritance; mutation and chromosomal aberrations; Human genetics - methods of study, genetic disorders. DNA as a genetic material - its structure and repliaction; structure of RNA and its role in protein synthesis; Gene expression - transcription and translation in prokaryotes and eukaryotes; regulation of gene expression, induction and repression - housekeeping genes; nuclear basis of differentiation and development; oncoenes. Basics of Recombinant DNA technolgy; cloning; gene bank; DNA fingerprinting; genomics principles and applications, transgenic plants, animals and microbes. Unit V Morphology of Plants and Animals

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Morphology - root, stem and leaf, their sturucture and modification; Inflorescence, flower, fruit, seed and their types;Description of Poaceae, Liliaceae, Fabaceae, Solanaceae, Brassicaceae and Asteraceae. Morphology of animals - salient features of earthworm, cockroach and rat; tissue systems, structure and function of tissues - epithelial, connective, muscular and nervious. Practical 1. Study of parts of Compound Microsope 2. Study of mitosis in onion root tip and animal cell (grasshopper) 3. Study of meiosis in onion flower buds, and testis iof grasshopper. 4. Study of cyclosis in leaf cell of Hydrilla, or Tradescantia and in Paramoecium. 5. Study of cell wall components ( cellulose, lignin, suberin and mucilage). 6. Study of mitochondria by staining with a Janus Green.

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

7. Study of specimens and their identification with reason - Bacteria, Oscillator, Spirogyra, Rhizopus, mushroom/bracket fungi, yeast, liverwort, moss, fern, Pinus, one monocotyledon, one dicotyledon and lichens. 8. Study of characters of specimens and identification with reason - Amoeba, Hydra, Liver - Fluke, Ascaris, Leech, Earthworm, Prawn, Silk moth honey bee, snail, Starfish, Dogfish, Rohu, Frog, Lizards, Pigeon/ any other bird and rabbit/ any other mammal. 9. Study of squamous epithelium, muscle fibres, nerve cells and mammalian blood film through temporary/permanent slides. 10. Study of external morphology of earthworm, cockroach, from and rat through models.

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PTU/DEP/Paramedical/September 2005/Detailed Syllabus

SEMESTER I

DRIT-105: Communication & Soft Skills

UNIT I Essentials of Grammar: · · · · UNIT II Office Management: · · · · · · · UNIT III Letter & Resume Writing: · · · · · · · UNIT IV Presentation Skills: · · · · · · · · · · · UNIT V Interview Preparation: · · · · · · Types of Interview Preparing for the Interviews Attending the Interview Interview Process Employers Expectations General Etiquette

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Parts of Speech Punctuation Vocabulary Building Phonetics

Types of Correspondence Receipt and Dispatch of Mail Filing Systems Classification of Mail. Role & Function of Correspondence MIS Managing Computer

Types of Letters-Formal / Informal Importance and Function Drafting the Applications Elements of Structure Preparing the Resume Do's & Don'ts of Resume Helpful Hints

Importance of Presentation Skills Capturing Data Voice & Picture Integration Guidelines to make Presentation Interesting Body Language Voice Modulation Audience Awareness Presentation Plan Visual Aids Forms of Layout Styles of Presentation.

· · UNIT VI

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

Dressing Sense Postures & Gestures

Group Discussion & Presentation: · · · · · Definition Process Guidelines Helpful Expressions Evaluation

(Note: Every student shall be given 15 minutes. of presentation time & 45 minutes of discussion on his/ her presentation.) The student will be evaluated on the basis of : his / her presentation style Feedback of Faculty & Students General Etiquette Proficiency in Letter Drafting / Interview Preparation The paper is internal and at least 3 tests will be taken. Best 2 of 3 shall account for final grades (70% Test & 30% Presentation) SEMESTER I

DRIT-106: Practical

Anatomy and Physiology & Related Pathology General and Radiation Physics Dark Room Techniques Introductory Biology Internal Assessment: 25 Marks : : : : 20 marks 20 marks 20 marks 15 marks

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PTU/DEP/Paramedical/September 2005/Detailed Syllabus

SEMESTER II Theory

DRIT-201: General Radiology

Upper Limb: · Finger individual and as a whole hands- carpal tunnel- writs fore-elbow- head of radius- humerus shoulder joint- acrtomie clavicular- scapula dlavicular joint. Lower Limb: · Toes-foot- calcaneum- ankle joint- leg knees- patella- femur- inter condylar notch. Hip & Pelivs: · Hip-neck of femur- theature procedure- for hip pinnig of reduction- pelvis saros iliac joints- public bones- acetablum- sp.p. nailing and platting. Vertebral column: · Curves- postures- relative levls- atlanto occipital region- odontoid process, cervical spine- cervicothoracic spine- thorace- lumber spine- lumbo-sacral spin- sacrum coccyx, scoliosis- kyphosis ­ Flexion and extension ­ Lordiosism abduction and adduction etc. Bones and the Throax: · Sterum- Ribs, elavicles. Skull: · Land marks, plans- cranium- facial bones, maxilla Mandible- Zygomatic ­ T.M. Joints, mastoidspetrous bones- Optic foremen- sella trunica ­ P.N.S. styloids process, juglar foremen. Internal auditory canal, base of skull, C.V. junction- Orbits.

Chest: · Chest in teleraography- Chest spine & portable, Erect and decubits view & apicogram, odelka camera or minature chest radiography Abdomen: · Preparation, indication and contra- indication acute abdomen ­ pregnancy abdomen for multiplicity maturity and foetal abnormality, abdomen supine and erect decubities study. Soft tissue radiography: · Neck & Breast etc. Dental radiography: · Dental X-ray of maxillary and mandibular teeth, occulusal- view of maxilla, madnible and nasal bones, panromax, cephalometery. Infantogram and invertogram Practical Taking X-ray of all the parts of the human body as the theory syllabus

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PTU/DEP/Paramedical/September 2005/Detailed Syllabus

SEMESTER II

DRIT-202: Special Investigation Radiology

Theory General pathology in radiation therapy: Pathology: · Defination, cell growth ­ cell deformities ­ cell damage- defence mechanism cell repair. Neoplasia: · Bengin & malignant including its mode of growth and metastasis. Causes of Disease: · Congential ­ traumatic- metabolic and deficiency ­ infection (micro- organism) immunization. Bloods diseases: · Leukaemias, Anaemias Radiotherapy: · Radiation treatment- methods ­ external radiation, use and application of radiation Radiotherapy techniques for: · Skin disease , Disease in system: respiratory, alimentary, urinary reproductive (including Brest, nedorcine, nervous) Special procedural and related contrast media, Contrast Media, Emergencies in radiology department Urinary tract: I.V.P. Retograde pyelograpy- cystourtherography Billiary tract: · Oral cheloecystograph- trnas heptic percutaneous cholanigraphy, pre-operative cholangiography, T-tube cholangiography. E.R.C.P. Gastronitnestial tract: · Ba.. swallow- Ba.. meal, upper GIT Ba. Meal following through B.a enema. Ba double contrast enema Female genital tract: · Hystro salpingography and pelvimetry Angiography: · cariotid angiography, femoral arteriography, aortography, selective angiograpy, cardiac catherization. CNS: · Ventriculography, Myelography,Pheumoencephalography & Shuntography

Tomography: · Principal, Equipment and types of movement in tomography Venography: · Splenoprotovenography & Superior venography, Lymphanhgiography Mammography Radioculography, Dacrocystography, Sialography, Sinography, Nasopharyngography, Laryngography Bronchography, Arthography, Discography, Introduction to Ultrasonography, Computerised tomography, scanning and magnetic resonance Imaging

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Radiography-Special investigation & Radiography Practical Radiography-Special investigation & radiotheraphy: Radiography in various positions for all the special radiological procedures using cantrast media, as per the syallbus Positioning and treatment of various Cancer patients using (a) Prescribed filters/Wedges (b) protecting various organs Prepration of Radium/ Cobalt applicator/ mould etc.

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SEMESTER II

DRIT-203: Electrocardiography & Techniques

Theory Introduction: · Introductio to Electrocardiography;History;psychological basis of E.C.G; conuction Velocity; Electrophysiology Central termical of Wilson;augmentation,Esophagea leads,Pathway of Activation;Vector Cencept Normal Electrogram: · Atrial complexex ; P-R interval; QRS Complex; S.T Segment; T-Wavw; U-Wave; Q-T interval; Electrical Axis; Heart Position; Interpretation of an ECG; How to record and ECG etc. Abnormal Electrocardiogram: · Abnormal P-Wave Interventricular Conduction Defects, RBBB, LBBB, Incomplete LBBB, LAHB,LPPHB; Non-specific Interventricular Condition Defects, Bilateral Bundle, Branch Block, Trifasicular Blocks WPW Syndrome; Lawn-ganong-Levine Syndrome;Mahim by Pass;Hypertrophy;Right Ventricular Hypertrophy (RVH); Pulmonary embolism;Chronic Obstructive lung Disease (COLD),Biventricular Hypertrophy;Overload Concept;Diastolic Overload(volume overload) etc. Coronary artery disease: · Ischaemial Injury, infraction, subtle, atypical, non-specific Patterns: ocnductoin defecs and infraction; localisation of infraction: vpm and acute myocardial infraction; artial infraction; VCG in myocardial infraction; atrial infraction; VCG in myocardial infraction, coronary insufficiency etc. Exercise test: · Type of exercise test ; termination exercise; exercise protocols in tredmill testing; escalation of exercise test results; preparation of patient for exercise testing etc. Drugs and electrolytes: · Digitalis; potassium; Hypokalemia; calcium; quinidine effect; phenothiazines; Anthracylines; cerebrovascular accidents; hypothermia; pericarditis ; myocarditis; neuromuscular diseases; heart trauma; malignancy involving heart; electrical alternans; negative U0vaves; liquid protein diet; anaemia etc; Disorders of cardiac- rhythm: · Distribuance of impulse formation; distribance of impulse conduction; secondary disorders of rhythm; physiology of cardiac rhythm; Automaticity; conductivity; A-V nodes; sinus rhythm; sinus tachycardia; sinus bradycardia; sinus arrhythmia; sino atrial block; partial Sa block; complete SA block; causes of Exit block; atrial extrasystoles; Blocked atrial premature beats; cause of Atrial tachycardia (PAT); Chaotic Atrial Rhythm; Atrial flutter; Atrial fibrillation; Superventicular techncardias (SVt); ventricular rhythm; ventricular tachy-cardia (VT) ventricular fibrillation; paraarrhythamias; parasystole; group beating ; AV-Disoocation; torsade de points ; sick sinus syndrome etc. ECG as a clue to clinical diagnosis: · Pulmonary stenoriss tricuspid atresia; atrial spetal defect; ventricular septal defect; ebstein anomaly; corrected trtransporation of great vessel; mirror image dextrocarida; anomalous brigin of left coronaro artery; Rheumatic fever: mitrial value prolapse; athetetes heart; cardiac pacing etc. Practical: Use of electro-cardiogram machine and recording etc as per the syllabus

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SEMESTER II

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

DRIT-204: Advanced Diagnostic Techniques

Ultrasound Scanning Principles Display of Images, Modes Doppler Ultrasound Duplex Ultrasound Endosonography Mammography Equipment Positioning & Projections Xero ­ Radiography Scintigraphy Radio nucleids Equipment Examination Procedure Patient Preparation and Position Magnetic Resonance Imaging Principles Equipment MR Signal Image Characteristics Pulse Sequences Advantage & Precautions

SEMESTER II

DRIT-205: Practical

General Radiology Special Investigation Radiology Electrocardiography & Techniques Advanced Diagnostic Techniques Internal Assessment: 25 Marks 20 Marks 20 Marks 20 Marks 15 Marks

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PTU/DEP/Paramedical/September 2005/Detailed Syllabus

B.Sc. Medical Lab Technology (BSCMLT)

SEMESTER I

BSCMLT-101: Microbiology

GENERAL MICROBIOLOGY Introduction and Brief History of microbiology: Definition,History and relationship of micro-org. to man Safety measures in microbiology Care and maintenance of laboratory equipment[3] Culture media : (a) Preparation of various media (b) Standardization and use Sterilization : (a) Definition (b) Different methods and principles ­Moist heat ,dry heat,Radiation &filtration (C) Autoclave - its structure ,functioning , control & indicators Antiseptics and disinfectants ; Definition , types ,mode of action & propertiesUses of disinfectant & antiseptics ,testing efficiency Glassware : Description of glass ware ,its use ,handling and care,Decontamination and disposal of contaminated material General characteristics and classification of important bacteria and fungi VIROLOGY Introduction to virology Physiochemical characteristics of viruses Diseases caused by different viruses and mode of infection PARASITOLOGY Introduction to medical parasitology and safety measures General characters and classification of protozoa of Medical Importance Morphology, Life cycle and laboratory diagnosis of Intestinal Protozoa- Amoebae and Giardia

SEMESTER I

BSCMLT-102: Anatomy & Histotechnology

Introduction to Histopathology and laboratory organization Laboratory equipment ,its uses and maintenance Laboratory hazards and safety precautions Anatomy and physiology of human body: General organization , synopsis of all systems Cell organization and function : Structure & function of all cell organelles Skeletal system : Structure and function of all individual bones and joints ,movement of joints ,Skeletal muscles , Cardiac muscles ,smooth muscles , muscles of upper arm & anterior compartment of thigh (their name, attachments , functions and nerve supply) Blood : Functions of blood , composition of blood ,plasma & its functions ; Blood clotting (mechanism ,clotting factors ) ; Morphology of red blood cells , their function and development ,Haemoglobin ,anaemia ; WBC :classification development & functions ; platelets : morphology &functions ; Blood groups , blood transfusion and transfusion reactions Respiratory system : Structure of respiratory pathway ,function of respiratory tract ,cough reflex ,intraplerual pressure, mechanism of breathing and respiration , muscles of respiration ,vital capacity , tidal volume ,inspiration ,reserve volume and residual volume l. Reception, recording and labeling of histology specimens Fixation and various fixatives Processing of histological tissues for paraffin embedding Embedding and embedding media Decalcification -various methods Introduction to exfolative cytology with special emphasis on female genital tract(PAP smear, cone biopsy) Solvents, mordents, accelerators and accentuators

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SEMESTER I

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

BSCMLT-103: Haematology and Clinical Pathology

Introduction to hematology : laboratory organization Definition , importance , important equipment and chemicals, various tests performed,

Composition and function of blood : Definition of blood , composition of blood (cells, plasma /serum ) Formation of blood : Erythropoiesis ,Leucopoiesis , Thrombopoiesis Anticoagulants :Definition , uses, different types , mode of action, their merits and demerits Morphology of normal blood cells : Normal morphology , morphology in diseases Collection and preservation of blood :Different methods of collection , preservation ,changes in stored blood Normal and absolute values in hematology: RBC count , WBC count ,Platelet count ,DLC value , Hb , MCH ,MCV ,MCHC , ESR, PCV Blood film : Different types , Methods of preparation ,Staining Romanwsky stains : Principle of staining , Different stains ,their composition and preparation , method of staining

SEMESTER I

BSCMLT-104: Biochemistry

Introduction to medical lab technology : General introduction Role of medical lab technologists, ethics, responsibility, safety measures and first aid. Cleaning and care of general laboratory glassware and equipment. Distilled water :Types of distilled water plants , preparation & storage Analytical balance: Principal ,Working & maintenance ; Preparation of reagents : Formulation and preparation ; Standard solutions: Various std. solutions used , their preparation ; storage of chemicals . Units of measurements: S.I units: Definitions, conversions; Measurement of volume : Strength , Normality , Molarity, Molality: volumetric apparatus, calibration of volumetric apparatus Definitions : Mole, molar and normal solutions (preparation , Standardization ) ; pH ( Definition ,Pka value, Example , Derivation of Henderson-Hasselbalch equation) ; Buffer solutions( Definition , preparation of important solutions), pH indicators ( pH papers , universal & other indicators ) ;pH measurement :different methods (pH paper , pH meter , principle of pH meter , structure ,working and maintenance.

SEMESTER I

BSCMLT-105: Introductory Biology

Unit I Living World Biology & Its Branches; relationships with other sciences; scientific methods in Biology; historical breakthroughs; scope of biology and career options; role of Biology in dispelling myths and misbelieves; characters of living organisms, (elementary idea of metabolism, transfer of energy at molecular level, open and closed systems, omoeostasis, growth and reproduction, adaptation, survival, death). Origin and evolution of life - theories of evolution; evidence of evolution; sources of variations ( mutation, recombination, genetic drift, migration, natural selection); concept of species; specification and isolation (geographical and reproductive); origin of species. Unit II Diversity of Life Variety of living organisms, Systematic; need, history and types of classification (artificial, natural, polygenetic); biosystematics; binomial nomenclature; Two kingdom system, Five

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kingdom System, their merits and demerits, status of bacteria and virus; botanical gardens and herbia; zoological parks and museums. Unit III Cell and Cell Division Cell as a basic unit of life - discovery of cell, cell theory, cell as a self - contained unit; procaryotic and eukaryotic cell; unicellular and multicellular organisms; tools and techniques ( compound microscope, electron microscope and cell fractionation); Ultrastructure of prokaryoytic and eukaryotic cell - cell wall, cell membrane - unit membrane concept (flauid mosaic model); membrane transport; cellular movement (exocytosis, endocytosis); cell organelles and their functions - nucleus, mitochondria, plastids, endoplamasic reticulum, Gogli complex, lysosomes, lysosomes, microtubules, centriole, vacuole, cytoskeleton, cilia and flagella, ribosomes. Molecules of cell; inorganic and organic materials - water, salt, mineral ions, carbohydrates, lipids, amino acids, proteins, nucleotides, nycleic acids (DNA and RNA); Enzymes (Properties, chemical nature and mechanism of action); vitamins, hormones and steroids. Unit IV Genetics Continuity of life - heredity, variation; mendel's laws of inheritance, chromosomal basis of inheritance; other patterns of inheritance - incomplete dominance, multiple allelism, quantitative inheritance. Chromosomes - bacterial cell and eukaryotic cell; parallelism between genes and chromosomes; genome, linkage and crossing over; gene mapping; recombination; sex chromosomes; sex determination; sex linked inheritance; mutation and chromosomal aberrations; Human genetics - methods of study, genetic disorders. DNA as a genetic material - its structure and repliaction; structure of RNA and its role in protein synthesis; Gene expression - transcription and translation in prokaryotes and eukaryotes; regulation of gene expression, induction and repression - housekeeping genes; nuclear basis of differentiation and development; oncoenes. Basics of Recombinant DNA technolgy; cloning; gene bank; DNA fingerprinting; genomics principles and applications, transgenic plants, animals and microbes. Unit V Morphology of Plants and Animals Morphology - root, stem and leaf, their sturucture and modification; Inflorescence, flower, fruit, seed and their types;Description of Poaceae, Liliaceae, Fabaceae, Solanaceae, Brassicaceae and Asteraceae. Morphology of animals - salient features of earthworm, cockroach and rat; tissue systems, structure and function of tissues - epithelial, connective, muscular and nervious. Practical 1. Study of parts of Compound Microsope 2. Study of mitosis in onion root tip and animal cell (grasshopper) 3. Study of meiosis in onion flower buds, and testis iof grasshopper. 4. Study of cyclosis in leaf cell of Hydrilla, or Tradescantia and in Paramoecium. 5. Study of cell wall components ( cellulose, lignin, suberin and mucilage). 6. Study of mitochondria by staining with a Janus Green.

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PTU/DEP/Paramedical/September 2005/Detailed Syllabus

7. Study of specimens and their identification with reason - Bacteria, Oscillator, Spirogyra, Rhizopus, mushroom/bracket fungi, yeast, liverwort, moss, fern, Pinus, one monocotyledon, one dicotyledon and lichens. 8. Study of characters of specimens and identification with reason - Amoeba, Hydra, Liver - Fluke, Ascaris, Leech, Earthworm, Prawn, Silk moth honey bee, snail, Starfish, Dogfish, Rohu, Frog, Lizards, Pigeon/ any other bird and rabbit/ any other mammal. 9. Study of squamous epithelium, muscle fibres, nerve cells and mammalian blood film through temporary/permanent slides. 10. Study of external morphology of earthworm, cockroach, from and rat through models.

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

SEMESTER I

BSCMLT-106: Communication & Soft Skills

UNIT I Essentials of Grammar: · · · · Parts of Speech Punctuation Vocabulary Building Phonetics

UNIT II

Office Management: · · · · · · · UNIT III Letter & Resume Writing: · · · · · · · UNIT IV Presentation Skills: · · · · · · · · Importance of Presentation Skills Capturing Data Voice & Picture Integration Guidelines to make Presentation Interesting Body Language Voice Modulation Audience Awareness Presentation Plan

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Types of Correspondence Receipt and Dispatch of Mail Filing Systems Classification of Mail. Role & Function of Correspondence MIS Managing Computer

Types of Letters-Formal / Informal Importance and Function Drafting the Applications Elements of Structure Preparing the Resume Do's & Don'ts of Resume Helpful Hints

· · · UNIT V

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

Visual Aids Forms of Layout Styles of Presentation.

Interview Preparation: · · · · · · · · UNIT VI Group Discussion & Presentation: · · · · · Definition Process Guidelines Helpful Expressions Evaluation Types of Interview Preparing for the Interviews Attending the Interview Interview Process Employers Expectations General Etiquette Dressing Sense Postures & Gestures

(Note: Every student shall be given 15 minutes. of presentation time & 45 minutes of discussion on his/ her presentation.) The student will be evaluated on the basis of : his / her presentation style Feedback of Faculty & Students General Etiquette Proficiency in Letter Drafting / Interview Preparation The paper is internal and at least 3 tests will be taken. Best 2 of 3 shall account for final grades (70% Test & 30% Presentation)

SEMESTER I

BSCMLT-107: Practical

Microbiology Anatomy & Histotechnology Haematology & Clinical Pathology Biochemistry Introductory Biology Internal Assessment: 25 Marks - 15 Marks - 15 Marks - 15 Marks - 15 Marks - 15 Marks

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SEMESTER II

BSCMLT-201: Microbiology

I GENERAL MICROBIOLOGY Principle of staining methods and preparation of reagents Aerobic and anaerobic culture methods General characters and nature of antigen and antibody Principle of antigen antibody reaction Collection, transportation and processing of clinical samples for microbiological investigations Principle and mode of action of antibiotics and chematherapeutic agents for bacteria and fungi Care and handling of laboratory animals Laboratory organisation, management, recording of results and quality control in microbiology II VIROLOGY Isolation of viruses in laboratory by tissue culture, Embryonated eggs and different laboratory animals, cell and tissue culture technology, Animal cell lines Principles of different serological tests used in Virology III Parasitology Morphology and diagnosis of Oral vaginal flagellates Trichomonas, E. Gingivalia Morphology and life cycle of Haemoprotozoa Material parasite including falciparum Laboratory diagnosis of Material infection General characters and classification of Medical Helminthology Morphology and life cycle of Nematodes(Intestinal) Ascaris, Enterobious, Ancylostoma, Trichuris, Strongloides Laboratory diagnosis of intestinal nematode infection Compound microscope optical systems,magnification and maintenance

SEMESTER II

BSCMLT-202: Anatomy & Histotechnology

Body fluids and their significance Cardiovascular system Alimentary system, mechanism and physiology of digestion and absorption Liner structure and function Urinary system Genital system- male and female Nervous system Spleen, lymph node and recticuloendothelial system Endocrine glands and their functions Microtomes various types, their working principle and maintenance Microtomes Knives and Knife sharpening Practical section cutting, cutting faults and remedies Routine staining procedures, mounting and mounting media Dye chemistry, theory and practice of staining Use of controls in various staining procedures Collection, processing and staining of cytological specimen

SEMESTER II

BSCMLT-203: Haematology and Clinical Pathology

Quality assurance in hematology Hb : Definition ,synthesis and breakdown Haemoglobinometry- various methods of estimation of Hb, errors involved and standardization of instrument for adaptation for Hb estimation Haemocytometry Procedure for cell count(visual and electronic), Red cell count, leukocyte count, platelet count. Errors involved and means to minimize such errors Physiological variation in Hb , PCV , TLC , platelets Erythrocyte sedimentation rate, factors influencing ESR, various methods of estimation and their significance Haemocrit value by macro and micro methods their merits and demerits Routine examination of urine Examination of biological fluids such as CSF etc. Examination of semen

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SEMESTER II

BSCMLT-204: Biochemistry

Radio isotopes and their use in biochemistry. Osmosis, dialysis, surface tension Urine analysis(Qualitative)for sugar, proteins, bile pigments, Ketone bodies, porphoblinogen, faecal occult blood, bile salts Collection and recording of biological specimens, separation of serum plasma preservation and disposal of biological samples/materials Basic statistics(mean, SD, CV, normal distribution, probability) Volumetric analysis preparation of standard acid and base solutions, chloride estimation

SEMESTER II

BSCMLT-205: Computer Application

Introduction to Computers Block diagram of a computer & overview of its working Interconnections of various peripherals with computers Input/Output & Secondary storage device Classified of programming languages Classification of computers Familiarization with operating system Introduction to Computer Operating System (Dos,Windows95/XP) Introduction to DOS structure, system files, batch files & configuration files Booting the system from floppy and hard disk Brief introduction to DOS, Internal and External commands Familiarization with windows structures, its use and application Prepation of Documents through word processing Idea of Text Editors like Microsoft Word, write etc. Opening a document Preparing documents, inserting diagrams and tables Editing documents Character, Word and line editing Margin setting, Paragraph alignment Block operations Spell checker Saving a document Printing a document Information Presentation for Decision making using Spreadsheet (Excel) Application of spread sheet Structure of spreadsheet Preparing spreadsheet for simple data and numeric operations Using formulae in spreadsheet operations Making tables, sorting and quering Creation of graphs, Pie charts, bar charts Printing reports Computer Aided Drafting (CAD) Making simple drawings using features of CAD and conforming the drafting specifications Saving and retrieving drawings Dimensioning Lettering Plotting drawings Practicles (4 hrs/ week) Indentification of various parts of the PC Demonstration of dis-assembly and assembly of PC and interconnection of Input and Output devices to PC Installation of DOS and simple exercise on TYPE, REN,CD,MD,TREE,COPY,BACKUP commands Grouping commands with batch files Disk diagnose, Correction of partitioning Installation of windows 95 Familiarization with start menu, taskbar icons, windows explorer Control panel of settings Getting hardware recognized Installation of MS-office MS-Word, Basics of Letterwriting,tempalates,wizards,formatting documents Creating Graphics, tables,mail merge,etc.. using MS-Word

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Building a sample worksheet using MS-Excel Formulas for calculations, sorting etc.. Creating Lotus 1-2-3 sheet Formulas, ranges and fuctions of Lotus 1-2-3 simple Graphics Importing and exporting graphics through CAD Drawing of geometric figures

SEMESTER II

BSCMLT-206: Practical

Microbiology Anatomy & Histotechnology Haematology and Clinical Pathology Biochemistry Computer Application Internal Assessment : 25 Marks - 15 Marks - 15 Marks - 15 Marks - 15 Marks - 15 Marks

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SEMESTER III

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

BSCMLT-301: Microbiology

Identification of Bacteria- Micrococci, staphylacocci pneumococci, corynebacteria, Escherichia, Klebsiella, Enterobacter, Proteus, Providencia, Salmonella, Shiqella, Arizona, Citrobactor,yesinia, Pseudomonas, Vibrio, Haemophilus, Mycobacteria, Buccella, Bordetella, Bacillus, Clostridia, Anaerobic cocci, Neisseria, Treponema, Borrelia, Leptospria, Mycoplasma, Ricketessia, Chlamydia, Tric agents Identification of Pathogenic and Non Pathogenic fungi Condida, Gyptococci, Dermato phytes, spcerotrichoums, Histoplasma, Blastomyces, Coccidiodies, Para caccidiodes, Dematiaceous fungi, Mycetoma, Actinomyces, Nocardia and common laboratory contains. Biochemical tests used for identification of bacteria and fungi Different staining techniques used in virology Use of Embryonated eggs in clinical virology Morphology and life cycle of haemoflagellates : leishmania, Trypansomes and their laboratory diagnosis Morphology, life cycle and lab diagnosis of tissue and blood nematodes filaria, trichmella, sracunculus Morphology, life cycle and laboratory diagnosis of intestinal cestodes Tanenia, Echinococcus, H. Nana, D.Latum culture techniques for protozoa, amoeba, Giardia, Leishmania Culture methods for Helminthis, Hookworm roundworm

SEMESTER III

BSCMLT-302: Basic Cellular Pathology & Allied Technology

Study of body tissues- Epithelial tissue, connective tissue including bone and cartilage, muscular tissue Study of various systems- Circulatory system, alimentary system, digestive system including liver, pancreas and gall bladder, Respiratory system Microscopy - Working principle, maintenance and applications of various types of microscopes -Darkground microscope, polarizing microscope, phase contrast microscope, Interference microscope, U.V. light microscope, micrometry Metachromasis and Metachromatic dyes Haematoxylin stain, its importance in histology. Stains, cytological preparation with special emphasis on MGG, PAPANTICOLOU stains. Special stains like PAS, Mucicarmine, Alcian blue, Schmorl, Acid phosphatase

SEMESTER III

BSCMLT-303: Haematology

History and discovery of blood group system ABO and Rhesus blood group system Compatibility tests in blood transfusion, complications and hazard of blood transfusion Laboratory investigation of transfusion reactions and mismatched transfusions Preparation of packed cells and various fractions of blood for transfusion purposes Bone marrow aspiration, composition and functions Staining of bone marrow smear and preparation of histological section

SEMESTER III

BSCMLT-304: Biochemistry

Colorimetery, Spectrophotometry, Flamephotometry, Atomic absorption spectroscopy,Electrometric determination of Na+ and k+, Chromatography, Electrophoresis, Radioimmunoassay (RIA) and ELISA

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SEMESTER III

BSCMLT-305: Practical

Microbiology Basic Cellular Pathology & Allied Technology Haematology Biochemistry : 20 Marks : 20 Marks : 15 Marks : 20 Marks

Internal Assessment : 25 Marks

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SEMESTER IV

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

BSCMLT-401: Microbiology

Antimicrobial sensitivity testing and assay methods for body fluids Antimicrobial susceptibility testing for mycobacteria Preparation and standardization of antigens and antisera. Principles of animal cell culture and their use in virology Use of common Laboratory animals in viral diagnosis Egg counting Technique Preparation of stains and staining procedures of malaria Identification of different plasmodium species Preparation of media and maintenance of cultures of E. histolytica, Giardia, Leishmania,

SEMESTER IV

BSCMLT-402: Basic Cellular Pathology & Allied Technology

Study of body tissues-Nervous tissue, glands, epithelial and endocrine. Study of various systems- urinary systems, system of endocrine glands, reproductive system, nerve endings and organs of special senses. Carbohydrates and amyloid -special stains and procedures Connective tissues, trichrome staining and other special stains for muscle fibbers, elastic, and reticule fibbers and collages fibers. Principles of metal impregnation technique Demonstration and identification of minerals and pigments. Cytological screening and quality control in cytology laboratory.

SEMESTER IV

BSCMLT-403: Haematology

Hemoglobin pigments and their measurement Abnormal hemoglobin and their means of identification and estimation LE cell estimation, various methods of its demonstration Homeostatic mechanism and theories of blood coagulation Physiochemical properties of coagulation factors Screening coagulation procedures Quantitative assay of coagulation factors

SEMESTER IV

BSCMLT-404: Biochemistry

Introduction, properties and simple metabolism of carbohydrates, proteins and fats, Nucleic acids and Enzymes(introduction, General properties)

SEMESTER IV

BSCMLT-405: Practical

Microbiology Basic Cellular Pathology & Allied Technology Haematology Biochemistry : 20 Marks : 20 Marks : 15 Marks : 20 Marks

Internal Assessment: 25 Marks

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SEMESTER V

BSCMLT-501: Microbiology

Preservation of microbes and lyophilization methods Total and viable counts of bacteria Testing of disinfectants Rideal Walker Chick Martin and In use tests. Preparation and standardization of vaccines and immunization Bacteriological examination of water, milk, good and air. Nosocomial infections and sterility testing of I/V fluids and processing of various samples for hospital infections Toxin Antitoxin assays and pathoqenicity tests Principles of serological techniques used in virology-HA, HA, Had, SRH,RPHA, IHA, CFT,CIEP. Mode of transmission of viral agents Morphology and life cycle of free living Amoebae, Balantidium, Toxoplasma Diagnosis of Morphology and life cycle of trematodes- Schistosomes, Intertinal flukes, blood flukes, wing flukes Serological and immunological techniques used in diagnosis of parasitic infections-Gel diffusion, IHA, IFA, ELISA,

SEMESTER V

BSCMLT-502: Histopathology

Handling of fresh histological specimen(tissues) cryo/frozen sections of fresh and fixed tissues, freeze drying. Lipids, identification and demonstration Micro-organisms in tissues- various staining technique for their demonstration and identification Nucleic acids, DNA and RNA special stains and procedures Cytoplasmic constituents and their demonstration Cervical cytology-basis ofdetection of maligrant and premalignant lesions Hermoral assessment with cytologic techniques and sex chromatis and pregnancy tests. Cells and organs of immune system Immunoglobulins, antibodies and humoral immune response Allergy Rheumatological diseases and investigations.

SEMESTER V

BSCMLT-503: Haematology

Definition and classifications of anaemia Laboratory investigation for megaloblastic anaemia Laboratory investigation for iron deficiency Anaemia Laboratory investigation for haemolytic anaemia including classification and causes Leukemia: definition and classification (General & FAB ) Cytachemical staining procedure in various haemopioetic disorders.

SEMESTER V

BSCMLT-504: Biochemistry

Principles of assay procedures for biological materials Total proteins, albumin, Glucose, Urea, Uric acid, Creatinine, Cholesterol, Bilirubin. Sodium, Potassium, Chloride, Calcium and phosphorus. PBI, 17- Ketosteroids, Barbiturates

SEMESTER V

BSCMLT-505: Practical

Microbiology Histopathology Haematology Biochemistry Internal Assessment: 25 Marks

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: 20 Marks : 20 Marks : 15 Marks : 20 Marks

SEMESTER VI

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

BSCMLT-601: Microbiology

Epidemiological markers of microorganism serotyping, Bacteriophage and Bacteriocine typing methods Lab diagnosis of common Bacterial infections viz:- pyogenic infecttions, Respiratory tract infections, Meningifis, Diptheria, Whooping Cough, Gas gangrene, food poisoning, Enteric fever, Acute diarrhea diseases, cholera, Urinary tract infection, Tuberculosis, Leprosy, Plague, Anthrax, Typhus fever, syphilis, Gonorrhea and other STD's Serological Tests-Widal, ASO, LFT, CRP, Rosewaller, brucella agglutination, cold agglutiration, VDRL, TPHA, PTA-ABS Lab diagnosis of fungal infections Superficial dermatophyte fungal infections, Candidiases, creptococosis, Pulmonary infections, Mycetoma, other deep mycotic infections, subcutaneous fungal infections subcutaneous fungal infections spozotrichosis, chromoblastomycosis, Eye and Ear fungi infections Serological tests for fungal infections and skin tests Advanced techniques in microbiology ELISA, RIA, CCIEA, Co-agglutination GLC, HPLC etc. Rapid diagnostic methods and Automation in Microbiology. Principles of Serological techniques used in virology- ELISA, RIA, IF, Immuno peroxidase test Prevention of viral disease Immunity in viral infection Introduction to Entomology Identipcation of Adultworms- mosquitoes, flies, ticks and fleas Animal care, handling and uses in parasitology. Preparation of parasitic antigens, antigens and antisera Handling and operating of sophisticated equipment.

SEMESTER VI

BSCMLT-602: Histotechnology

Tissues requiring special treatment i.e eye ball, Bone marrow biopsy, undercalcified bones. Neuropathologic techniques Enzyme histochemistry demonstrations of phosphatases, dehydrogenases oxidases and peroxidases etc. Electron microscope, working principle, components and allied techniques for electron microscopy, ultra-microtomy Museum techniques Aspiration cytology principles, indications and utility of the technique with special emphasis on role of cytotechnician in FNAC clinics Infection and immune system Cancer Immunology Tissue typing for kidney transplant

SEMESTER VI

BSCMLT-603: Haematology

Laboratory tests for assessing bleeding disorders Laboratory investigation for disseminated intravascular coagulation(DIC) Mechnaism of fibrinolysis test for fibrinolysis Platelet function tests and their interpretation Techniques available for cytogenetic studies Use of Radioisotopes in hematology Safety measures for handling Radioisotopes

SEMESTER VI

BSCMLT-604: Biochemistry

Glucose tolerance test, insulin tolerance test, gastric analysis, ,xylose absorption test, clearance test for renal functionEnzymes Acid and alkaline phosphates, AST, ALT, Amylase and lactate dehydrogenase, CPK Analysis of calculi and CSF Quality control of clinical investigations, Automation in clinical biochemistry laboratory, laboratory organisation management and maintenance of records.

SEMESTER VI

BSCMLT-605: Practical

Microbiology Histopathology Haematology Biochemistry Internal Assessment: : 20 Marks : 20 Marks : 20 Marks : 15 Marks 25 Marks

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PTU/DEP/Paramedical/September 2005/Detailed Syllabus

B.Sc. ( Clinical Microbiology ) BSCCMB

SEMESTER I

BSCCMB-101: Human Anatomy

13. Introduction to Anatomy & Histology, Structure of cell, epithelial tissue, muscular tissue, nervous tissue. 14. Skeletal System, Structure of bones, types of bones, Bones of cranium, face vertebral column upper and lower limbs, fracture of bones, various movements of joints. 15. Muscular System, Structure and types of muscles in human body, important muscles and their group action. 16. Circulation System, Structure of heart, names and position of main blood vessels. 17. Lymphatic System, Lymph vessels, lymph nodes and lymphoid organs, their structure & functions. 18. Digestive systems. Parts of gastrointestinal tract and associated glands. 19. Respiratory System. Parts of Respiratory System. 20. Urinary System. Parts of Urinary System. 21. Endocrine System. Various endocrine glands. Thyroid. Parathyroid. Adrenal glands pituitary pancreas. Thymus and sex glands. 22. Reproductive System. Male & female Reproductive organs. 23. Skin and sense organs. Eye, Ear, Nose. Taste Buds 24. Nervous System. Parts of brain, spinal cord, peripheral nerves. PRACTICAL: Identification of bones & other organs of the body and viva voce as per theory syllabus.

SEMESTER I

BSCCMB-102: Human Physiology

1 Blood. Composition and function of blood, haemopesis, blood coagulation. Blood groups, body fluid. 2 Cardiovascular Systems. Circulation of blood. function of heart and blood vessels. Control of heart rate, pulse, regulation of blood pressure, blood volume. 3 Respiratory system. Function of lungs, mechanism of breathing and exchange of gases in the lungs, regulation of respiration, Respiration disorder like anoxia.dyspnea cyanosis etc.artificial respiration lung function tests. 4 Digestive Systems. Digestion of food in mouth, stomach & small intestines. Absorption offood, function of liver function tests. 5 Excretory Systems. Structure & function of kidney and urinary bladder. Mechanism of urine formation. disorders of kidney. 6 Endocrine Glands Functions of various endocrine glands and hormones secreted by them. 7 Reproductive Systems. Physiology & female reproductive organs. 8 Nervous System. Neuron & its function, function of central nervous system, Autonomies nervous system , physiology of vision, hearing & olfaction.

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SEMESTER I

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

BSCCMB-103: Clinical Biochemistry

Ch1: Carbohydrates : Carbohydrates metabolism, Glycogenesis, Glycolysis, Regulation of Blood Glucose Concentration, Diabetes Mellitus, Glycosuria, Glucose Tolerance Test Ch2: Lipids; Introduction, Simple Lipids, Compound Lipids, Derived Lipids, Essential Fatty Acids, Ketone bodies formation Ch3: Proteins Definition, Classification, Structure of Proteins, Denaturation of Proteins, Important tests of Proteins, Estimation of Proteins, Albumin, Globulin, Glycoproteins, Protein Estimation, Estimation of Total Proteins, Albumin & Globulin, Estimation of Blood Urea, Serum Creatinine Estimation Serum Uric Acid Estimation Ch4: Liver Function Tests, Serum Bilirubin Estimation, Serum protein Estimation Ch5: Enzymology, Introduction, Definition & Classification, Properties, Factors influencing Action of Enzymes, Diagnostic Value of Serum Enzymes, Mechanism of Enzyme Action

Ch:6 Minerals, Vitamins & Automation Minerals ­ Physiological Functions, Sources, Daily Requirements of Calcium, Phosphorus, Magnesium, Sodium, Patassium, Chlorine, Sulphur, Iron, Copper, Zinc Vitamins, Definition, Classification, Fat soluble Vitamins, Vitamin A,D,E,K Water soluble vitamins , Vitamin C, Vitamins of B Complex, Diseases Biochemical aspects of hormones ­ Role of Insulin PRACTICALS: Estimation of glucose and glucose tolerance test Estimation of Total groupins ­ albumin, globulins Renal Function Tests Liver Function Tests Lipid Profiles Estimation of Na, R, Column by Flame Photometry Estimation of Enzymes: Acid & Alkaline Phosphatase, Amylase, Serumamino ­ transferase Uric acid estimation.

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SEMESTER I

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

BSCCMB-104: Bacterial Morphology & Classification

Unit I (i) (ii) (iii) Unit II (i) (ii) (iii) Unit III (i) (ii) (iii) Unit IV (i) (ii) (iii) Unit V (i) (ii) Classification of Bacteria. Economic importance of eukaryotis and Viruses. Bacterial nutrition : Nutritional requirement and growth factor. Bacterial Growth curve. Growth of Bacteria under extreme condition (Psychrophiles, thermophiles and acidophiles) Structures of internal to the cell wall like. Cytoplasmic inclusion. Cytoplasmic membrane and cytoplasm. Chemical structure of Bacterial cell wall. Gram positve and Gram negative bacteria. Structure and function of flagella, capsule, cilia and pilli Introduction and history of Microbiology. Difference between prokaryotic and eukaryotic cells. Bacterial shapes.

SEMESTER I

BSCCMB-105: Introductory Biology

Unit I Living World Biology & Its Branches; relationships with other sciences; scientific methods in Biology; historical breakthroughs; scope of biology and career options; role of Biology in dispelling myths and misbelieves; characters of living organisms, (elementary idea of metabolism, transfer of energy at molecular level, open and closed systems, omoeostasis, growth and reproduction, adaptation, survival, death). Origin and evolution of life - theories of evolution; evidence of evolution; sources of variations ( mutation, recombination, genetic drift, migration, natural selection); concept of species; specification and isolation (geographical and reproductive); origin of species.

Unit II Diversity of Life Variety of living organisms, Systematic; need, history and types of classification (artificial, natural, polygenetic); biosystematics; binomial nomenclature; Two kingdom system, Five kingdom System, their merits and demerits, status of bacteria and virus; botanical gardens and herbia; zoological parks and museums. Unit III Cell and Cell Division Cell as a basic unit of life - discovery of cell, cell theory, cell as a self - contained unit; procaryotic and eukaryotic cell; unicellular and multicellular organisms; tools and techniques ( compound microscope, electron microscope and cell fractionation); Ultrastructure of prokaryoytic and eukaryotic cell - cell wall, cell membrane - unit membrane concept (flauid mosaic model); membrane transport; cellular movement (exocytosis, endocytosis); cell organelles and their functions

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- nucleus, mitochondria, plastids, endoplamasic reticulum, Gogli complex, lysosomes, lysosomes, microtubules, centriole, vacuole, cytoskeleton, cilia and flagella, ribosomes.

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

Molecules of cell; inorganic and organic materials - water, salt, mineral ions, carbohydrates, lipids, amino acids, proteins, nucleotides, nycleic acids (DNA and RNA); Enzymes (Properties, chemical nature and mechanism of action); vitamins, hormones and steroids. Unit IV Genetics Continuity of life - heredity, variation; mendel's laws of inheritance, chromosomal basis of inheritance; other patterns of inheritance - incomplete dominance, multiple allelism, quantitative inheritance. Chromosomes - bacterial cell and eukaryotic cell; parallelism between genes and chromosomes; genome, linkage and crossing over; gene mapping; recombination; sex chromosomes; sex determination; sex linked inheritance; mutation and chromosomal aberrations; Human genetics - methods of study, genetic disorders. DNA as a genetic material - its structure and repliaction; structure of RNA and its role in protein synthesis; Gene expression - transcription and translation in prokaryotes and eukaryotes; regulation of gene expression, induction and repression - housekeeping genes; nuclear basis of differentiation and development; oncoenes. Basics of Recombinant DNA technolgy; cloning; gene bank; DNA fingerprinting; genomics principles and applications, transgenic plants, animals and microbes. Unit V Morphology of Plants and Animals Morphology - root, stem and leaf, their sturucture and modification; Inflorescence, flower, fruit, seed and their types;Description of Poaceae, Liliaceae, Fabaceae, Solanaceae, Brassicaceae and Asteraceae. Morphology of animals - salient features of earthworm, cockroach and rat; tissue systems, structure and function of tissues - epithelial, connective, muscular and nervious. Practical 1. Study of parts of Compound Microsope 2. Study of mitosis in onion root tip and animal cell (grasshopper) 3. Study of meiosis in onion flower buds, and testis iof grasshopper. 4. Study of cyclosis in leaf cell of Hydrilla, or Tradescantia and in Paramoecium. 5. Study of cell wall components ( cellulose, lignin, suberin and mucilage). 6. Study of mitochondria by staining with a Janus Green. 7. Study of specimens and their identification with reason - Bacteria, Oscillator, Spirogyra, Rhizopus, mushroom/bracket fungi, yeast, liverwort, moss, fern, Pinus, one monocotyledon, one dicotyledon and lichens. 8. Study of characters of specimens and identification with reason - Amoeba, Hydra, Liver - Fluke, Ascaris, Leech, Earthworm, Prawn, Silk moth honey bee, snail, Starfish, Dogfish, Rohu, Frog, Lizards, Pigeon/ any other bird and rabbit/ any other mammal. 9. Study of squamous epithelium, muscle fibres, nerve cells and mammalian blood film through temporary/permanent slides. 10. Study of external morphology of earthworm, cockroach, from and rat through models.

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SEMESTER I

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

BSCCMB-106: Communication & Soft Skills

UNIT I Essentials of Grammar: · · · · Parts of Speech Punctuation Vocabulary Building Phonetics

UNIT II

Office Management: · · · · · · · UNIT III Letter & Resume Writing: · · · · · · · UNIT IV Presentation Skills: · · · · · · · · · · · UNIT V Interview Preparation: · · · · · · · Types of Interview Preparing for the Interviews Attending the Interview Interview Process Employers Expectations General Etiquette Dressing Sense

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Types of Correspondence Receipt and Dispatch of Mail Filing Systems Classification of Mail. Role & Function of Correspondence MIS Managing Computer

Types of Letters-Formal / Informal Importance and Function Drafting the Applications Elements of Structure Preparing the Resume Do's & Don'ts of Resume Helpful Hints

Importance of Presentation Skills Capturing Data Voice & Picture Integration Guidelines to make Presentation Interesting Body Language Voice Modulation Audience Awareness Presentation Plan Visual Aids Forms of Layout Styles of Presentation.

· UNIT VI

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

Postures & Gestures

Group Discussion & Presentation: · · · · · Definition Process Guidelines Helpful Expressions Evaluation

(Note: Every student shall be given 15 minutes. of presentation time & 45 minutes of discussion on his/ her presentation.) The student will be evaluated on the basis of : his / her presentation style Feedback of Faculty & Students General Etiquette Proficiency in Letter Drafting / Interview Preparation The paper is internal and at least 3 tests will be taken. Best 2 of 3 shall account for final grades (70% Test & 30% Presentation)

SEMESTER I

BSCCMB-107: Practical

Human Anatomy Human Physiology Clinical Biochemistry Bacterial Morphology & Classification Introductory Biology Internal Assessment: 25 Marks

: 10 Marks : 10 Marks : 20 Marks : 20 Marks : 15 Marks

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SEMESTER II

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

BSCCMB-201: General Bacteriology Overview of Bacteriology Structure and Function of Procaryotes Nutrition and Growth of Bacteria Growth of Bacterial Populations Control of Microbial Growth The Diversity of Procaryotic Metabolism Regulation and Control of Metabolic Activities Procaryotes in the Environment Important Groups of Procaryotes Maintenance of bacterial cultures. Classification of bacteria.

SEMESTER II

BSCCMB-202: General Microbiology Theory History of Microbiology, Microbial World Defined, Tools Used in Microbiology, Microbial Life, Viruses , Microbial Growth, Microbial Genetics, Control of Microbes, Antibiotics, Microbe Versus Host, The Immune System, Bacterial & Fungal Diseases, Viral Diseases Practical 1. Microorganisms and their size 2. Use and care of the microscope 3. Smear preparation and simple staining 4. Gram stain 5. Special stains 6. Pure culture technique 7. Culture media and culturing microorganisms from the environment 8. The effects of incubation temperatures 9. The effects of ultraviolet light 10. The effects of gaseous environments 11. The use of antiseptics and disinfectants 12. The transformation experiment 13. Diagnostic bacteriology 14. Antibiotics 15. Serology

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SEMESTER II

BSCCMB-203: Biochemistry

Unit I (i) (ii) (iii) Unit II (i) (ii) (iii) Unit III (i) (ii) (iii) Unit IV (i) (ii) (iii) Unit V (i) (ii) (iii) Nucleic Acid : Structure of purine and pyrimidine bases, Nucleotide and Nucleosides. DNA and RNA structure and properties. Vitamins (Fat and water soluble). Lipids : classification and properties. Enzyme : Classification, mechanism of enzyme action, catalyzed reaction. Enzyme inhibition. Carbohydrate : Structure, properties, chemical reactions and functions. Amino acid : Essential and nonessential amino acids with structure and function. Proteins : Primary, Secondary Tertiory and Quaternary (overview) Basic aspects of Bioenergetics. Entropy, enthalpy and concept of free energy. Thermodynamic law first and second. Introduction to Biochemistry and role of medical microbiologist. Ethics. Safety measure and hazards in clinical biochemistry lab and first aid.

Practical (i) (ii) (iii) (iv) (v) (vi) (vii) (viii) Blood glucose and its determination by different methods. Glucose tolerance test. (GTT) Estimation of blood urea. Estimation of blood creatinine. Estimation of blood cholestrol. Estimation of serum calcium and phosphorus. Estimation of plasma protein. Determination of Protein in Urine.

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SEMESTER II

BSCCMB-204: Basic Cellular Pathology Introduction. General concepts of disease? Scope of Pathology and the role of Pathologist in clinical medicine. Disease classification. The normal cell Cell response of injury Causes of cell damage Changes associated with water accumulation in the cell Changes associated with fat accumulation Nature of the degenerative changes Hyaline degeneration Accumulations of normal substances in the cell Cell death and necrosis Connective tissue: normal tissue and effects of disease

SEMESTER II

BSCCMB-205: Practical General Bacteriology General Microbiology Biochemistry Basic Cellular Pathology Internal Assessment : 15 Marks : 15 Marks : 25 Marks : 20 Marks : 25 Marks

Paramedical Syllabus Manual : 44 / 144

SEMESTER III

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

BSCCMB-301: Nutrition & Microbial Metabolism

Unit I (i) (ii) Unit II (i) (ii) Unit III (i) (ii) Unit IV (i) (ii) Unit V (i) (ii) Photosynthesis : Oxygenic and anoxygenic photosynthetic prokaryotes. Nitrogen assimilation : Nitrogen fixation, Nitrification and Denitrification. Carbohydrate metabolism : glycolysis, krebs cycle, HMP cycle and its regulation. Lipid metabolism : Biosynthesis of Lipid and degradation of fatty acid. Nutritional types of microorganism. Collection, Transportation and processing of clinical sample for microbiological investigation. Bacterial growth, generation time. Growth kinetics - Synchronous Batch and continuous cultures. Measurement of growth and factor affecting growth. Microbial Nutrition : Nutritional requirement of microorganism C, N Sources and Vitamins. Effect of temperature, pH, O2, CO2 and osmotic pressure on growth.

Practical (i) (ii) Neutrilization and cleaning of glasswares, sterilization. Preparation of culture media like Nutrient agar, Mc Conkey and uses.

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SEMESTER III

BSCCMB-302: Methodology of Staining

Unit I (i) (ii) (iii) Unit II (i) (ii) (iii) Unit III (i) (ii) (iii) Unit IV (i) (ii) (iii) Unit V (i) (ii) (iii) Staining properties of bacteria. Gram positive and gram negative stain. AFB Staining. Microbiological stains and staining techniques. The wet mount, hanging drop and fixation. Staining of smear, simple, negative, differential and structural staining. Cultural characteristics, Spread plate, Streak plate and Pour plate. Aerobic and Anaerobic culture. Storage of stock culture. Types of media with their general or special use and example. Microbial culture : Mixed and pure cultures. Isolation of pure culture. Microbial Media : Preparation of media for bacteriological culture (Nutrient agar, Mc Conkey) Specimen collection from patients, clinics and hospitals. Specimen collection for epimiological investigation.

Practical 1 2 3 4 Preparation of stains, Gram positive, gram negative and Acid fast Base staining. Isolation of culture by streak plate and pour plate. Growth on agar slant, agar slab and broth culture. Drug sensitivity test.

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SEMESTER III

BSCCMB-303: Microbial Molecular Genetics

Unit I The study of Microbial genetics : (i) (ii) Unit II Genotypic changes : (i) (ii) (iii) Unit III Bacterial Recombination : · Unit IV (i) (ii) Unit V (i) The Regulation and expression of gene Activity : · Lac operon. Transduction (Zinder and Lederbery) : generalized and specialized transduction. Transformation (Griffith experiment) Conjugation (Luria Delbruck) Mutation (Types) Its Occurs. Repairing. Inheritance of characteristics and variability. Phenotype & genotypic.

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SEMESTER III

BSCCMB-304: Applied Immunology

Unit I (i) (ii) (iii) Unit II (i) (ii) (iii) Unit III (i) (ii) Unit IV (i) (ii) Unit V (i) · · · Electrophoresis and Chromatography Principle, Technique and application. Thin layer chromatography (TLC) Polyacrylamide Gel Electrophoresis (PAGE), SDS - PAGE Agrose Gel Electrophoresis Basic and advanced Serological techniques and its application. Serological tests : Widal, VDRL Resewaller, Brucella aggutination and Cold agglutination. ELISA technique and its application. RIA - Principle and its application. Autoimmunity. Laboratory test for detection of Antigen and Antibodies. Autoimmune disorders. Basic concepts : Introduction of Immunology and its application. Antigen, Antibody. Immune complex.

SEMESTER III

BSCCMB-305: Practical

Nutrition & Microbial Metabolism Methodology of Staining Microbial Molecular Genetics Applied Immunology Internal Assessment : : : : : 15 Marks 20 Marks 20 Marks 20 Marks 25 Marks

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SEMESTER IV

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

BSCCMB-401: Bacterial Pathogenes and Associated Dieses

Unit I (i) (ii) (iii) Unit II (i) Mode of infection, description, pathogenecity, incubation period and toxic effect of: · · · Unit III (i) (ii) (iii) Unit IV (i) Mode of infection, pathogenecity, incubation time and toxicgenecity of: · · · Unit V (i) Pathogenecity and toxicgenecity of: · · · Salmonella. Escherichia coli. Vibrio. Mycobacteria. Anthraxbacillus. Brucella. Pathogenic properties of bacteria. Host Parasite interation in bacterial infection. Antibacterial Defence of the Host. Staphylococcus. Streptococcus. Pneumococcus. Normal Microflora of human, Bodyskin, Gastrointestinal tract, Respiratory system. Infection : Source and mode of infection for man. Portals entry.

SEMESTER IV

BSCCMB-402: Lab Diagnosis of Microbial Diseases

Unit I The Role of the Laboratory in the dignosis and control of infection. (i) (ii) Unit II (i) (ii) Unit III (i) (ii) Unit IV Lab Diagnosis of fungal infection (i) (ii) Unit V (i) (ii) Rapid diagnostic methods. Automation in Microbiology. Skin, Nails and Hair Leprosy, staphylococcal and streptococcal infection, superficial and systemic dermatomycosis. Eye and Ear fungi infection. Sexually transmitted diseases : Syphilis, Gonorrhea and other STD's. AIDS and Typhus fever. Circulatory system disease : Plague and Malaria. Central nervous system disease : Meningitis, Tetanus and Poliomyelitis. Respiratory tract disease : Diptheria, tuberculosis and common cold. Alimentary tract disease : Typhoid, food poisoning, cholera and dysentry.

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SEMESTER IV

BSCCMB-403: Virology

Unit ­ I General morphology and ultra structure of Viruses: Capsids- Helical Symmetry, icosahedral symmetry and complex symmetry. Envelope: Glycoprotein and matrix protein Viral genome: their types and structure Unit ­ II Cultivation of Viruses in embryonated eggs, experimental animals and cell culture: primary and secondary cell culture, suspension cell culture and monolayer cell cultures. Assays of viruses: physical and chemical methods of assays (protein nuclei acid, radioactivity traces, electrons microscopy, plaque method, pock counting method, end point method and infectivity of plant viruses). Serological methods: haemagglutination haemagglutinationinhibitation, complement fixation, immunofluroscence assays (IFA) ELISA, RIA. Unit ­ III Plant viruses: Recent advances in classification of plant viruses Life sciences and other details of TMV and mosaic virus, potato virus X General idea about cyanophages, actinophages and mycoviruses. Unit ­ IV Bacterophages: Classification, Morphology and ultrastructure One step growth curve ( Latent period, eclipse period and burst size) Life cycle: Lytic and Lysogenic cycles of bacteriophages Unit ­ V Animal viruses; classification and nomenclature Life cycles and other details of DNA viruses: herpes, adeno and SV40 Life cycle and other details of RNA viruses: Retroviruses, oncogenic viruses and lentiviruses (HIV), picorna, ortho myxo and paramyxo.

SEMESTER IV

BSCCMB-404: Mycology

MICOLOGY 1. Classification of Fungi 2. Growth and isolation 3. Mycoses (all types) 4. Laboratory diagnosis of mycotic diseases. 5. Immunity in fungal diseases and value of immuno diagnosis. 6. Role of mycotoxin 7. Antifungal agents 8. Epidemiology of fungal diseases.

Practical

-KOH & LPCB Preparation -Staining Techniques -Culture of Fungi -Slide Culture -Basic identification techniques.

SEMESTER IV

BSCCMB-405: Practical

Bacterial Pathogenes and Associated Dieses Lab Diagnosis of Microbial Dieses Virology Mycology Internal Assessment : 25 Marks

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-

10 25 20 20

Marks Marks Marks Marks

SEMESTER V

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

BSCCMB-501: Haematology

Formation of blood cells, Sites of Blood formation, Erythropoiesis, Leucopoiesis The Red Blood Cell, Structure of RBC, Functions of RBC and Values, Definition of Anaemia and Polycythaemia, Normal Hb and Abnormal Hb Classification of Anaemia, Iron Deficient Anaemia, Megaloblastic Anaemia, Haemolytic Anaemia White Cells, Structure and Functions of WBC, Normal WBC values and types, Leukemias- Definition, Platelets: Structure and function. Blood Groups, Clinical Significance of blood groups, Blood Transfusion

PRACTICAL:Blood film preparation and various stainings To study Morphology of various types of Lecuocytic series of Normal and Leukemic Cells. To study Morphology of Normal and Abnormal Red blood cells and Platelets Blood grouping and Rh typing.

SEMESTER V

BSCCMB-502: Parasitology

Introduction to medical parasitology and safety measures Laboratory Techniques for Intestinal Parasites. Laboratory Techniques for Blood Parasites Examination of Urine, Aspirates, Body Fluids and Tissue Biopsies Intestinal Protozoan Parasites Serology and Culture of Parasites Blood and Tissue Protozoa Common Intestinal Helminths of Humans Common Blood and Tissue Helminths Quality Control Procedures in Parasitology SEMESTER V

BSCCMB-503: Serology Diagnosis

Introduction to Serology and Serodiagnostic Procedures. Principles of Immunological Reactions. Serodiagnosis. Collection and Preperation of Specimen. Serological test for syphilis (STS). Aggulation Tests, C-reactive protein test (CRP), Rheumatoid arthritis test (RA), Seriodiagnosis of streptococcal infection, Serodiagnostic tests for miscellaneous disorders. Immunological tests for Pregnancy.

SEMESTER V

BSCCMB-504: Blood Banking

Chapter 1: Basic Principles of Blood Banking , Introduction, Organization & Planning, Documentation Chapter 2: Blood Groups Introduction, ABO Blood Groups, Principle of ABO Group Determination, Techniques of Blood Grouping, Rh,Blood Groups, Techiques of Rh grouping Chapter 3: Selection of Donors & Collection of Blood. Screening of Blood for VDRL, Hepatitis B and C, AIDS Chapter 4: The Compatibility Test, Major & Minor Cross Match, Coomb's Test Anticoagulants Chapter 5: Blood Components, Packed Cell Volume & Plasma, Froze Red Blood Cell PRACTICAL: Collection of Blood and Screening of Blood Blood grouping and Rh typing Coomb's test Complete haemogram

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SEMESTER V

BSCCMB-505 Practical

Haematology Parasitology Serology Diagnosis Blood Banking Internal Assessment 25 15 10 25 25

Paramedical Syllabus Manual : 52 / 144

SEMESTER VI

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

BSCCMB-601: Applied Microbiology

Normal flora of the human body Collection of clinical specimens for diagnosis and method of processing antibiotic susceptibility testing and its interpretation and reporting. Nosocomial infection -surveillance and control. Diseases transmitted through air, water, milk and food. Quality control in diagnostic microbiology. National programmes for control of infectious diseases. Etiology, laboratory diagnosis of infectious diseases of each system.

SEMESTER VI

BSCCMB-602: Automation & Computerization in Medical Microbiology

Unit I (i) (ii) (iii) Unit II (i) (ii) (iii) Unit III (i) (ii) (iii) Unit IV (i) (ii) (iii) Unit V (i) (ii) (iii) Medical Transcription : Introduction, Types of Medical documents, contents of medical case sheet. Goal of Medical Transcription training. Automation of Medical Microbiology Laboratory. Introduction to Internet. Internet basics of Microbiologist. Electronic Mail, electronic mail servers, Down loading file with anonymous FTP. Comptuer Application and their use in Medical Microbiology. Advantages of using computers in collecting data. Types of data processing, Authorization and report validation. Computer simulation. Computer linked to microbiological equipments. Fortran and application. Computer Fundamentals : Introduction to CPU, input drives, storage and output devics. Binary, decimal, octal and hexadecimal systems. DOS/Windows.

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SEMESTER VI

BSCCMB-603: Preventive Medicine & Health Care

Unit I (i) (ii) (iii) Unit II (i) (ii) (iii) Unit III (i) (ii) (iii) Unit IV Bacteriology of water, milk, food and air. (ii) (iii) Unit V (i) (ii) Bacterial examination of ice cream, shellfish and canned foods, milk bottles, crockery and cutlery. Bacteriological examination of air and environment dust. Collection of specimen. Counting and interpretation of results. Infection and control : Source of infection to men, mode of spread and their control by physical and chemical agents. Epidemiology : Control of community infection. Family planning and Child Health care programme. Air : Composition, pollution, vantilation and lighting. Floor space, Noise, Radiation air supply, Humidity and Housing. Sanitation : Excreation, Sanitation barrier, treatment and Disposed of Hospital waste. Water - source, uses, requirements, and pollution. Methods of Purification. Types of water and standards of water quality.

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SEMESTER VI

BSCCMB-604: Research Methodology

Introduction to Research: Definition, Scope, Limitations, and Types. Objectives of Research Research Process Research Designs Data Collection: Secondary Data, Primary Data, and Methods of Collection. Scaling Techniques: Concept, Types, Rating scales & Ranking Scales Scale Construction Techniques, Multi Dimensional Scaling. Sampling Designs: Concepts, Types and Techniques Sample Size Decision Theory of Estimation and Testing of Hypothesis Small & Large Sample Tests, Tests of Significance based on t, F , Z test and Chi-Square Test. Designing Questionnaire. Interviewing. Tabulation, Coding, Editing. Interpretation and Report Writing.

SEMESTER VI

BSCCMB-605: Practical

Applied Microbiology Automation & Computerization in Medical Microbiology Preventive Medicine & Health Care Research Methodology Internal Assessment : 25 Marks - 20 Marks - 20 Marks - 20 Marks - 15 Marks

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B.Sc. Biotechnology (BSCBT)

SEMESTER I

BSCBT-101: Introductory Biotechnology

Unit I Introduction: What is Biotechnology? What is agriculture? What is biology? What is biotechnology? History of biotechnology? What do you do with it? Why do it? Who does it? Science method to solve a problem. The role of biotechnology in our society. The role of genetic variation and natural selection in evolution. Applications of biotechnology Careers in biotechnology. Unit II Biochemistry and the Cell Atoms, Elements, Compounds Chemical bonds Life's key elements Relate 5 key elements to the essential compounds of all living organisms. Chemical reactions Organic compounds Proteins, Carbohydrates, Lipids, Nucleic Acids Compare and contrast the role of Proteins, Carbohydrates, Lipids, and Nucleic Acids in life. Enzymes Explain the role of enzymes in living organisms. Carbon - Oxygen cycle Cells & Basic structure Identify and locate cell structures and relate them to their functions. Using energy Discover the importance of glucose to living organisms. Fermentation Using a microscope to compare different types of cells.

Unit III Molecular Biology Nucleotides Key elements and molecules The structure of DNA. Base pairing The way in which genetic material is copied. DNA and RNA Describe the structure and function of RNA as is relates to protein synthesis. Explain how proteins are made. Replication, Transcription, Translation and Protein Synthesis TLW--Extract DNA from known substances. Restriction enzymes The value of gel electrophoresis in learning the genetic code.

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Amplification & Cloning

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

Unit IV Genetics Genes and Inheritance Genes and DNA Relate genes and heredity to DNA. Discover patterns of inheritance in living organisms. Homozygous and Heterozygous Dominance and Recessiveness Punnet squares F1, F2, F... Using a Punnet Square to determine patterns of inheritance in monohybrid (1-factor) and dihybrid (2factor) crosses. Problem solving Using a computer model to solve genetic/heredity problems. Isolate and fingerprint DNA to discover the infinite potential of the genetic code. Karyotype chromosomes to discover how sex and certain diseases are inherited. Disease and disorders Genetic counseling Unit V Microbiology Bacteria and viruses as pathogens The characteristics of bacteria. The characteristics of viruses. Hharmful versus helpful bacteria and viruses Antigen and Antibody Understanding how the immune system combats pathogens. Cultures, Sterile techniques Using sterile techniques to culture bacteria. Transformation, Plasmids and Conjugation Engineering with microbes Why bacteria and viruses are essential tools of biotechnology. Immune system How the immune response is used in the development of consumer products, industry, and medicine. Unit VI Applications of Biotechnology Plants Plant systems The anatomical and physical development of plants. Photosynthesis / Respiration Sexual vs. Asexual Reproduction Describe the principles of sexual and asexual reproduction in plants. Propagate plant tissue using tissue culture techniques. Animals Animal systems The anatomical and physical development of animals. Digestion / Rumination the digestion process in a ruminant animal. The principles of reproductions in animals. Reproduction Technologies * Artificial Insemination * Embryo Transfer * Embryo Sexing * Cloning Embryos

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The process of embryo sexing, splitting, and cloning. Recombinant DNA * BST * Genetic Engineering in Animals appropriate techniques in setting up animal tissue cultures.

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

Food Science Food composition Food safety Food preservation How biotechnology is used to insure the safety of food and food processing. The role of biotechnology in consumer foods. Fermentation * industrial * traditional Medical Antibody Antigen Using the interactions between antigen and antibody to determine hormone levels. Vaccines How vaccines provide immunity to diseases. Infectious diseases Viruses Blood typing Describe the production, use, and abuse of antibiotics. Describe the current research being done on HIV and cancer. The Human Genome Project The Human Genome Goals of the Human Genome Project Genetic Linkage Maps Polymorphic DNA Markers Physical Maps Sequence - Tagged Sites Integrating Genetic Linkage DNA Sequencing Ethical, Legal and Social Implications Unit VIII Ethical Issues and the Future The positives, negatives, and importance of biotechnology. What are the issues? Is there a right and wrong? Crime / forensics & DNA? Super humans and eugenics Is Jurassic Park possible? Environmental impact / "over-engineering" Saving endangered species Current issue in biotechnology. The ethics of genetic engineering and biotechnology.

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SEMESTER I

Unit I

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

BSCBT-102: General Microbiology

Microscope (optical, TEM and SEM) Experiments of Pasteur's. Sterilization : Methods (Dry heat, wet heat, radiation and chemical etc.). Unit II Various forms of Microorganism (PPLOs, Cocci, bacilli and spirilla) Concept of stains. Microbial cell wall. Gram positive and gram negative bacteria. Unit III Prokaryotic and Eukaryotic microbial cells. Gene transfer in micro-organism. Nutritional classification of micro-organism. Unit IV Classification and their role in bioprocesses of Biomolecules. Molecules involved in generation of mechanical stability - papetidoglycans, polysaccharides. - Membrane lipids. Unit V Molecular involved in information storage and retrieval - nucleic acid. Molecules executing mediator and catalytic functions - the protein. PRACTICAL Sugar in given sample. Extraction and separation of lipids. Estimation of Protein. Cleaning of glassware and sterilization. Preparation of media. Microbes from hands, tooth - scum and other body parts. Identification of isolation bacteria (Gram Staining or other staining methods.

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SEMESTER I

BSCBT-103: Biochemistry

Unit I (i) (ii) (iii) Unit II (i) (ii) (iii) Unit III (i) (ii) (iii) Unit IV (i) (ii) (iii) Unit V (i) (ii) (iii) Nucleic Acid : Structure of purine and pyrimidine bases, Nucleotide and Nucleosides. DNA and RNA structure and properties. Vitamins (Fat and water soluble). Lipids : classification and properties. Enzyme : Classification, mechanism of enzyme action, catalyzed reaction. Enzyme inhibition. Carbohydrate : Structure, properties, chemical reactions and functions. Amino acid : Essential and nonessential amino acids with structure and function. Proteins : Primary, Secondary Tertiory and Quaternary (overview) Basic aspects of Bioenergetics. Entropy, enthalpy and concept of free energy. Thermodynamic law first and second. Introduction to Biochemistry and role of medical microbiologist. Ethics. Safety measure and hazards in clinical biochemistry lab and first aid.

Practical (i) (ii) (iii) (iv) (v) (vi) (vii) (viii) Blood glucose and its determination by different methods. Glucose tolerance test. (GTT) Estimation of blood urea. Estimation of blood creatinine. Estimation of blood cholestrol. Estimation of serum calcium and phosphorus. Estimation of plasma protein. Determination of Protein in Urine.

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SEMESTER I

BSCBT-104: Introductory Biology

Unit I Living World Biology & Its Branches; relationships with other sciences; scientific methods in Biology; historical breakthroughs; scope of biology and career options; role of Biology in dispelling myths and misbelieves; characters of living organisms, (elementary idea of metabolism, transfer of energy at molecular level, open and closed systems, omoeostasis, growth and reproduction, adaptation, survival, death). Origin and evolution of life - theories of evolution; evidence of evolution; sources of variations ( mutation, recombination, genetic drift, migration, natural selection); concept of species; specification and isolation (geographical and reproductive); origin of species. Unit II Diversity of Life Variety of living organisms, Systematic; need, history and types of classification (artificial, natural, polygenetic); biosystematics; binomial nomenclature; Two kingdom system, Five kingdom System, their merits and demerits, status of bacteria and virus; botanical gardens and herbia; zoological parks and museums. Unit III Cell and Cell Division Cell as a basic unit of life - discovery of cell, cell theory, cell as a self - contained unit; procaryotic and eukaryotic cell; unicellular and multicellular organisms; tools and techniques ( compound microscope, electron microscope and cell fractionation); Ultrastructure of prokaryoytic and eukaryotic cell - cell wall, cell membrane - unit membrane concept (flauid mosaic model); membrane transport; cellular movement (exocytosis, endocytosis); cell organelles and their functions - nucleus, mitochondria, plastids, endoplamasic reticulum, Gogli complex, lysosomes, lysosomes, microtubules, centriole, vacuole, cytoskeleton, cilia and flagella, ribosomes. Molecules of cell; inorganic and organic materials - water, salt, mineral ions, carbohydrates, lipids, amino acids, proteins, nucleotides, nycleic acids (DNA and RNA); Enzymes (Properties, chemical nature and mechanism of action); vitamins, hormones and steroids. Unit IV Genetics Continuity of life - heredity, variation; mendel's laws of inheritance, chromosomal basis of inheritance; other patterns of inheritance - incomplete dominance, multiple allelism, quantitative inheritance. Chromosomes - bacterial cell and eukaryotic cell; parallelism between genes and chromosomes; genome, linkage and crossing over; gene mapping; recombination; sex chromosomes; sex determination; sex linked inheritance; mutation and chromosomal aberrations; Human genetics - methods of study, genetic disorders. DNA as a genetic material - its structure and repliaction; structure of RNA and its role in protein synthesis; Gene expression - transcription and translation in prokaryotes and eukaryotes; regulation of gene expression, induction and repression - housekeeping genes; nuclear basis of differentiation and development; oncoenes. Basics of Recombinant DNA technolgy; cloning; gene bank; DNA fingerprinting; genomics principles and applications, transgenic plants, animals and microbes. Unit V Morphology of Plants and Animals

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Morphology - root, stem and leaf, their sturucture and modification; Inflorescence, flower, fruit, seed and their types;Description of Poaceae, Liliaceae, Fabaceae, Solanaceae, Brassicaceae and Asteraceae. Morphology of animals - salient features of earthworm, cockroach and rat; tissue systems, structure and function of tissues - epithelial, connective, muscular and nervious. Practical 1. Study of parts of Compound Microsope 2. Study of mitosis in onion root tip and animal cell (grasshopper) 3. Study of meiosis in onion flower buds, and testis iof grasshopper. 4. Study of cyclosis in leaf cell of Hydrilla, or Tradescantia and in Paramoecium. 5. Study of cell wall components ( cellulose, lignin, suberin and mucilage). 6. Study of mitochondria by staining with a Janus Green.

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

7. Study of specimens and their identification with reason - Bacteria, Oscillator, Spirogyra, Rhizopus, mushroom/bracket fungi, yeast, liverwort, moss, fern, Pinus, one monocotyledon, one dicotyledon and lichens. 8. Study of characters of specimens and identification with reason - Amoeba, Hydra, Liver - Fluke, Ascaris, Leech, Earthworm, Prawn, Silk moth honey bee, snail, Starfish, Dogfish, Rohu, Frog, Lizards, Pigeon/ any other bird and rabbit/ any other mammal. 9. Study of squamous epithelium, muscle fibres, nerve cells and mammalian blood film through temporary/permanent slides. 10. Study of external morphology of earthworm, cockroach, from and rat through models.

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SEMESTER I UNIT I

BSCBT-105: Communication & Soft Skills

Essentials of Grammar: · · · · UNIT II Office Management: · · · · · · · UNIT III Letter & Resume Writing: · · · · · · · UNIT IV Presentation Skills: · · · · · · · · · · · UNIT V Interview Preparation: · · · · · · · Types of Interview Preparing for the Interviews Attending the Interview Interview Process Employers Expectations General Etiquette Dressing Sense

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Parts of Speech Punctuation Vocabulary Building Phonetics

Types of Correspondence Receipt and Dispatch of Mail Filing Systems Classification of Mail. Role & Function of Correspondence MIS Managing Computer

Types of Letters-Formal / Informal Importance and Function Drafting the Applications Elements of Structure Preparing the Resume Do's & Don'ts of Resume Helpful Hints

Importance of Presentation Skills Capturing Data Voice & Picture Integration Guidelines to make Presentation Interesting Body Language Voice Modulation Audience Awareness Presentation Plan Visual Aids Forms of Layout Styles of Presentation.

· UNIT VI

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

Postures & Gestures

Group Discussion & Presentation: · · · · · Definition Process Guidelines Helpful Expressions Evaluation

(Note: Every student shall be given 15 minutes. of presentation time & 45 minutes of discussion on his/ her presentation.) The student will be evaluated on the basis of : his / her presentation style Feedback of Faculty & Students General Etiquette Proficiency in Letter Drafting / Interview Preparation The paper is internal and at least 3 tests will be taken. Best 2 of 3 shall account for final grades (70% Test & 30% Presentation)

SEMESTER I

BSCBT-106: Practical

Introductory Biotechnology General Microbiology Biochemistry Introductory Biology Internal Assessment: 25 Marks - 10 Marks - 15 Marks - 25 Marks - 25 Marks

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SEMESTER II

BSCBT-201: Genetic Taxonomy & Genome

Unit I Nature of gene : chemical nature. Gene cistron relationship in Prokaryotes and eukaryotes. Gene families and pseudogens. Unit II Overlapping gene. Transgenes. Transposable elements. Unit III Gene Mutation. Physical and Chemical Mutagens. Molecular Mechanism. Unit IV Gene organisation (Principle) Separation Techniques : Northern and Southern. Unit V Gene libraries Ligation and selection technique. Selection by genetic recombination.

SEMESTER II

BSCBT-202: Enzymology

Unit I Enzyme : Introduction, classification, Protein and non protein enzymes. Catalystic reaction of enzyme. Kinetics of enzyme. Unit II In vitro activity of purified enzymes and their applications in industry. Enzyme uses in food processing, medicine, diagnostics and production of new compounds. Enzymes as research tool - modification of biological compounds with the help of enzyme. Practical Assays of Enzyme diagnostic. Kinetic studies on enzymes.

SEMESTER II

BSCBT-203: Immunology

Unit I Immune system : Immunity Organs of the immune system with function. Unit II Antigen - Antibody and their structure. Antigen antibody interaction. Unit III Humoral immunity. Cell mediated immunity (role of MHC and genetic restriction).

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Practical

Purification of antigens. Purification of antibodies. Conjugation and labeling of antibodies. ELISA RIA

SEMESTER II

BSCBT-204: Genetic Engineering

Unit I Concept of gene. Structure of gene. Significance of gene. Unit II Introduction of Genetic Engineering. Tools of Genetic Engineering : Vectors and Enzyme. Laboratory requirement and Safety of Genetic Engineering techniques. Unit III Introduction of Gene cloning. Purification and digestion of DNA. Insertion of DNA into vectors. Unit IV Insertion of recombinant DNA into host. Identification of hybrid clone. Unit V Genetic Engineering in Plants : Molecular breeding. Genetic Engineering in Medicine : Gene Therapy. Genetic Engineering in Forensic Science : DNA finger Printing.

SEMESTER II

BSCBT-205: Practical

Genetic Taxonomy & Genes Enzymology Immunology Genetic Engineering Internal Assessment: 25 Marks

: : : :

20 20 15 20

Marks Marks Marks Marks

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SEMESTER III

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

BSCBT-301: Fermentation Techniques

Unit I Introduction : Fermentation, Fermentor. Design of Fermentor. Unit II Local condition with in a fermentor. Ideal Fermentor. Unit III Types of Fermentors (Principle and Parts) Batch fermentor (BF) Continuous stirred-tank Fermentor (CSTF) Unit IV Tubular Fermentor (TF) (Principle and parts) Fluidiced bed Fermentor (FBF) Unit V Computer control of fermentation processes : Introduction. Instrument. Computer application in Fermentation Technology.

SEMESTER III

BSCBT-302: Cell Biology

Unit I Cell structure : Prokaryotic and Eukaryotic. Structure of Plasma membrane with different models. Structure of cell organelles (overview) Unit II Structure of chromosome. Structure of DNA and types. Different types of RNA. Unit III Cell Division : Mitosis. Meosis : The reduction division. Significance of DNA.

Practical Cell counting method - haemocytometer. Cytological preparations - Fixation, dehydration Staining. Squash in stain. Embedding and sectioning. Separation of cell types (from blood) Separation of cell organelles.

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SEMESTER III

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BSCBT-303: Tissue Culture

Unit I Introduction of Tissue culture. History of development. Primary and secondary culture. Unit II Anchorage and non Anchorage depends of growth. Growth kinetics and organ culture. Application of Tissue culture for gene expression.

SEMESTER III

BSCBT-304: Molecular Biology

Unit I:- Cell:- Cell organelles: Structure function, structure of mitochondria and organization of respiratory chain, organization of cytoskeleton and nucleic. Unit -II:- STRUCTURE & PROPERTIES OF DNA Discovery of DNA as the genetic material, Structure of DNA (A,B&Z forms ), concept & definition of the genome, C-value paradox, denaturation & renaturation of DNA, repetitive & non-repetitive DNA, reassociation kinetics. Cot curve, Rot curve, chemical & kinetic complexitv of DNA, supercoiling in DNA. Unit -III :- PROKARYOTIC AND EUKARYOTIC GENOME ORGANIZATION:-Organization of viral genome, organization of bacterial genome, chromosome structure in eukaryotes, nucleosome model, chromatin structure, satellite DNA, polytene chromosomes, lampbrush chromosomes, B chromosomes-Evolution of the gene concept, definition of gene, interrupted genes, multigene families & pseudogenes, overlapping genes, nested genes, open reading frames. Unit-IV:-DNA REPLICATION:- General features of DNA replication in prokaryotes & eukaryotes, enzymology of DNA replication, regulation of DNA replication Unit-V:- GENE MUTATION, REPAIR & RECOMBINATION:-Definition & types of mutation, mutagenic agents & their mode of action, reversion & suppression of mutation. DNA repair mechanisms : light dependent repair, excision repair, mis-match repair Post-replication repair, sos response. DNA recombination mechanisms : holiiday model, Rec A, Rec BCD, gene conversion SEMESTER III

BSCBT-305: Practical

Fermentation Techniques Cell Biology Tissue Culture Molecular Biology Internal Assessment : 20 Marks : 25 Marks : 20 Marks : 15 Marks : 25 Marks

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SEMESTER IV Unit I

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

BSCBT-401: Bio-chemical Techniques

Nature of Biological material. General properties of organic compounds. Hydrophilic and Hydrophobic groups in biological molecules. Unit II Perspectives of biological macromolecules : the repeating units in nucleic acids and proteins. Helicity, bending, looping, pleats, salt bridges and their determents. The basis for intermolecules interaction eg : enzyme substract and antigen antibody recognition. Unit III Energetics of a living body. Principle of Colorimetry and spectrophotometer. Strategies of light reception in microbes :- Plant and animals. Unit IV Intra and intermolecular interactions in biological systems. Spatial and charge compatibility as determination of such interaction. Unit V General spectroscopy of UV-Vis Fluorescence Atomic absorption

SEMESTER IV

BSCBT-402: Bio-physical Techniques

Unit I Principle & application of Light Microscope Phase Contrast Microscope Fluorescence Microscope SEM & TEM Unit II Principles & applications of paper chromatography Thin layer chromatography Affinity & Ion Exchange Chromatography HPLC Fixation Staining Unit III Principles of biophysical methods used for analysis of biopolymer structure. X-ray diffraction, NMR & ESR spectroscopy Unit IV Principles and Applications of traces techniques in Biology Radiation dosimetry Radioactive isotopes & half life on isotopes Scintillation spectrophotometry

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SEMESTER IV

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

BSCBT-403: Industrial Biotechnology

Unit I Application of Biotechnology in Agriculture. Biofertilizer and Nitrogen fixers. Bio-pesticides. Bio-weedicis Biotechnology. Unit II Application of Biotechnology in Medicine Vaccine Steroids transformation. Protein formation. Unit III Application of Biotechnology in Paper industry and plant petroleum. Unit IV Application of Biotechnology in Environment. Biotechnological inputs in producing good quality national fibres. Conventional fuel and their environmental impacts. Plant and animal wastes. Animal oil Unit V Application of Biotechnology in Food Industry. Beverages. Bakery Dairy Product. INDUSTRIAL APPLICATION PRACTICAL Study of Products which are manufactured in industry, on the basis of biotechnology principle.

SEMESTER IV

BSCBT-404: Instrumentation

Unit-1 :Centrifugation : principle, types, analytical and preparatory centrifugation, differential density gradient centrifugation, sedimentation and coefficient centrifuge and its application. Electrophoresis : principles, types and application (paper, starch, polyacrylamide and agarose electrophoresis. Chromatography : principle, types and application of paper, thin layer, ion exchange, gas chromatography and HPLC. Unit - 2 :Microscopy : light, phase contrast, interference & electron microscopy Radioactivity : saddy-fajans & russel group displacement law, half life period, disintegration or decay constant, transmutation or disintegration of elements. Radio carbon dating method, application of transmutation reaction (artificial radioactivity). Geiger muller counter, scintillation, liquid, crystal & gamma counter. Unit -3 :Basic principles of spectroscopy ; electromagnetic radiations, wave properties & parameters, electromagnetic spectrum spectroscopic methods in each region, types of spectra & interaction mechanisms.

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UV-vis spectroscopy, TR spectroscopy, nuclear magnetic resonance spectroscopv (NMR), electron spin resonance spectroscopy (ESR). Unit - 4 :Atomic spectroscopy - principle, instrumentation, preparation of samples, applications. Flame photometry. X-ray crystallography - principle, instrumentation and applications. ORD &. CD ; fundamental principle, cotton effect, birefringes, refractive indices. Application of ORD & CD for quantitative analysis, octant rule, haioketone rule. Principle, instrumentation & application of polarimetry,

SEMESTER IV

BSCBT-405: Practical

Bio-Chemical Techniques Bio-Physical Techniques Industrial Biotechnology Instrumentation Internal Assessment : 20 Marks : 15 Marks : 20 Marks : 20 Marks : 25 Marks

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SEMESTER V

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

BSCBT-501: Animal Biotechnology

Unit I General metabolism. Secondary metabolites/Products (insulin, Growth hormone, Interferon, factor VIII etc.) Expressing cloned proteins in animal cells, production and processing of chosen protein. Unit II Introduction to invitromethod : Terms and definition, growth regulators. Enbryo culture, embryo rescue after wide hybridization, and its application. Introduction to the process of embryogenesis and organogenesis and their practical application. Unit III Haploids and their application. Somaclonal variation and application. Clonal multiplication of elite species.

Practical

Practical application of genetic transformation (theory).

SEMESTER V

BSCBT-502: Plant Biotechnology

Unit I Introduction to protoplast isolation : Principle and application. Testing of viability of isolated protoplast. Various steps in the regeneration of protoplast. Unit II Introduction to somatic hybridization. Various method for fusing protoplast. Use of markers for selection of hybrid cells.

Practical

Practical application of somatic hybridization (theory).

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SEMESTER V

BSCBT-503: Environmental Biotechnology

Unit I Renewable and non renewable resources. Renewable should be bio-assimilable/bio degradable. Major consumer items : Food, fuel and fibres. Unit II Conventional fuel and their environmental impacts. Firewood Plant and animal wastes. Coal Gas Animal oil Unit III Modern fuels and their environmental impacts. Methogenic bacteria and biogas. Microbial hydrogen production. Conversion of sugars to ethanol. Cellulose degradation for combustible fuel. Plant based petroleum industry. Unit IV Biotechnological inputs in producing good quality national fibres. Microbiological quality of food and water. Unit V Biofertilizers. Treatment of municipal waste and industrial effluents. Degradation of pesticides and other toxic chemicals by micro-orgnism. Practical Practical application of tissue and organ culture (summarizing the practical application of all the above mentioned techniques in paper) only theory.

SEMESTER V

Unit I

BSCBT-504: Pharmaceutical Biotechnology

Production recombinant pharmaceuticals : Recombinant insulin Human Growth factor synthesis Recombinant factor VIII Unit II Synthesis of : Recombinant vaccine Recombine Protein Live recombinant vaccine Unit III Identification of genes responsible for human disease. Breast cancer Autosomal dominant and recessive disorders. X-linked disorders. Unit IV Gene therapy of somatic cell. Genetic Manipulation of Grem cell.

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Unit V Prospects and Ethics of gene therapy

SEMESTER V

BSCBT-505: Practical

Animal Biotechnology Plant Biotechnology Environmental Biotechnology Pharmaceutical Biotechnology Internal Assessment : : : : 20 20 10 25 Marks Marks Marks Marks

: 25 Marks

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SEMESTER VI

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

BSCBT-601: Bio-informatics

Introduction to Bioinformatics. Uses of Bioinformatics. Applications of Bioinformatics Websites for Bioinformatics. Fact file of Bioinformatics.

SEMESTER VI

Pharmacology.

BSCBT-602: Drug Design

Drug metabolism. Pharmacogenetics. Frontiers in pharmacology. Unit V Statistical methods in Pharmacology. Introduction to Research in pharmacology. Introduction to Pharmacoinformatics.

SEMESTER VI

BSCBT-603: Research Methodology

Introduction to Research: Definition, Scope, Limitations, and Types. Objectives of Research Research Process Research Designs Data Collection: Secondary Data, Primary Data, and Methods of Collection. Scaling Techniques: Concept, Types, Rating scales & Ranking Scales Scale Construction Techniques, Multi Dimensional Scaling. Sampling Designs: Concepts, Types and Techniques Sample Size Decision Theory of Estimation and Testing of Hypothesis Small & Large Sample Tests, Tests of Significance based on t, F , Z test and Chi-Square Test. Designing Questionnaire. Interviewing. Tabulation, Coding, Editing. Interpretation and Report Writing.

SEMESTER VI

BSCBT-604: Information Technology & Bioinformatics

Unit I Overview of computers. Operating system and networking concept (Windows, UNIX, Linux) Basics of internet and websites. Unit II MS-Office. Data structure and Algorithms.

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Bioinformatics Internet Applications

SEMESTER VI

BSCBT-605: Practical

Bio-informatics Drug Design Research Methodology Information Technology & Bioinformatics :20 :15 :20 :20 Marks Marks Marks Marks

Internal Assessment: 25 Marks

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PTU/DEP/Paramedical/September 2005/Detailed Syllabus

B.Sc.(Bio-Informatics) - BSCBI

SEMESTER I BSCBI-101: BIOINFORMATICS AN OVERVIEW. Introduction: Definition, Objectives, Importance, Historical Achievements, Databases- definion, types, analysis. Bioinformatics in world and in India. Brief review of macro molecules like DNA, RNA and Proteins. Brief review of Central Dogma, DNA sequencing. Websites: Introduction. Computers in biochemical research. Data Collection, Data Storage and Data Planning. Data Designing and building up databases. What is internet and role of internet in bioinformatics. What is WWW & introduction to websites. Enlisting of websites pertaining to macro molecules (DNA, RNA< Protein), sequencing (both DNA, Protein), Genome, Human Genome Project etc.. Advantages of Websites. Applications: In drug recovery, designing. Genome based therapy, Epidemiological studies, In Evolutionary biology. 3D Structural prediction of macromolecules. Mathematical modeling of life proceses. SEMESTER I BSCBI-102 GENERAL PRINCIPLES OF CHEMISTRY. Unit I a. Elements, compounds and Stoichiometry: Law of conservation of mass, Law of Constant composition or definite proportions, laws of multiple proportions, Gay Lussac's Law of gaseous volumes. b. Basic Atomic Structure: Particle and electrical nature of matter. Faraday's Law of electrolysis. Rutherford model of atom, Atomic no and mass no. Isotopes, Isobars & Isotones, electronic structure of atoms, Dual Character of Light, Heiseuberg uncertainity principle, debrogelie equation, Quantum numbers, Pauli's Exclusion Principle, Shapes of S and P orbitals, Aufbau Principle, Hunds Rule, Schrodinger's Wave Equation. d. Periodicity Atomic radir, Ionisation potential, Electron Affinity, Electronegativity. d. Properties of Gases ­ . Boyle's Law, Charles Law, Graham's Law of Diffusion Unit II : A. Thermodynamics 1st Law : Law of conservation of energy, types of reactions i.e. Isothermal process, Adiabatic Process, Isochoric Process, Exothermic & Endothermic Process. Eithalphy. B. Acids & Bases Different concepts regarding acides & bases, concept of PH C. Equilibrium Law of mass control, Law of Chemical Equilibrium D. Behaviour of Oxides Acidic Oxide, Basic Oxide, Amphoteric Oxide, Natural Oxide. E. Reaction of Organic Compound & Its Mechanism Electrophilic, Neucleophilic substitution and reaction. Unit III A. Properties of Solutions Colligative Properties of Solution. i) Lowering of vapour pressure ii) Elevation of boiling point iii) Depression of freezing point iv) Osmotic Pressure B. Types of Solution. Unit ­ IV A. Thermodynamics IInd Law - Spontaneous Processes, Entropy. B. Electro Chemistry ­ Electrochemical Cells, Voltic Cell, Cell Reaction, Cell Potential or e.m.f. SEMESTER I BSCBI-103 : Introduction to Bio-computing Unit - I Introduction to Computers

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What is Computer, Characteristics of Computer, Organization of Computer, generation fo Cmomputer, Types of Computers, History of Computers, Capability and Limitations of Computer. Introduction to Programming Languages Machine Language, Assembly Language and High Level Programming Languages. Algorithm and Flowcharts General Idea about algorithms, Flowcharts and Programs, Characteristics of Algorithms, Algorithm Development, Flowchart Symbols, Rules for drawing Flowcharts, Example of making Flowcharts, Advantages and Limitations of FlowCharts. Data Representation Introduction to Number System, Binary, Octal, Decimal and Hexadecimal number system, Conversion of numbers from one system to another. Input-Output Devices: Input Devices like Keyboard, Mouse, Scanner, MICR, OMR, OCR, Output Devices like VDU, Impact and Non ­ Impact Printers, Plotters , Sceonday Storage Devices like Floppy Disk, Hard Disk, CD. Unit II Introduction to Operating Systems PTU/DEP/Paramedical/September 2005/Detailed Syllabus Paramedical Courses Syllabus: 74 / 140 Objectives of Operating Systems, eneral idea about various types of O.S. , Single User and Multiuser OS, Real Time & On-line OS. Multiprogramming and Time sharing Operating Systems, Functions of Operating System. Introduction to MS-DOS Disk Drives, Structure of MS-DOS, Booting Process, Files and File name conventions, Difference between Internal & External DOS commands, Internal DOS commands : CLS, DIR, MD, CD, RD, DEL, COPY, RENAME, DATE, TIME, PATH, VER, VOL, TYPE. External DOS Commands: FORMAT, TREE, DISKCOPY, XCOPY, DISKCOMP. Comcept of Path, Wild Card Characters. Introduction to Windows Windows features, Desktop Icons, Various typs of Menues and Bars Like Title Bar, Menu Bar, Taskbar, Various types of Windows like Application Window, Dialog Window, Document Window, Explorer Window. Creating Files or Folders, Opening, Copying and Deleting files of folders, creating shortcut. Introduction to Unix UNIX structure ­ Kernel and Standard Utility Programme, File System ­ UNIC Directory, Files and Inodes, Some UNIX Commands like ­ pwd, cd, ls, cp, mv, rm, mkdir, rmdir, chmod, chgrp, cat, more, less, pg, who, whoami. Introduction to Linux: Linux Architecture and Features of Linux. UNIT ­ III Introduction to MS-Office MS-Word: Feature of Word Processor, Setting Margins and Columns, Font Style and Sizes, Text alignment, Margins & Guttters, Line spacing, Tabs, Headers and Footers, Page Break, Find & Replace, Page Setup, Character Formatting, Paragraph Formatting, Printing, Mail Merging. UNIT ­ IV Data Structure and Internet Data Structure Linear and Linked Allocation Techniques Stack ­ Definition, PUSH & POP Algorithms Queue Definition, Insertion and Deletion algorithm. Link List ­ General Idea about Singly and Doubly Linked List. Internet Internet usage, Web Browsers, Web Servers, Web Sites, URLS, Web Surfing, Access of E-mail, Chatting. OOPS Concepts: Introduction to Object Oriented Programming, Characteristics of OOPS, General Idea about Inheritance, Message Passing, Polymorphism, Operator and Function Overloading, Data abstracting, Classes and Objects. Unit ­ V Programming in C++ Introduction, Concept of Data Types, C++ data types, Constants and Variable, C++ Operators (Arithmetic, Relational, Logical and Conditional Operators), Arithmatic Expression, Assignment Statement, Flow of Control, Jump statement (goto, break & Continue), Branching Statement (If-then, It-Else-Then, Switch ), Looping Statement (While, Do-While, For), Nested Loops.

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SEMESTER I BSCBI-104 : CALCULUS, FUNCTION AND NUMBER SYSTEMS Topics which have been conferred during this session:Number System ­ Real numbers, Properties of real numbers, rational numbers, irrational numbers, Integers, Prime and Composite Numbers are widely covered with their problems. Complex Numbers ­ Complex numbers in the form o a+iB, representation of complex numbers by points in a plane, algebra of complex numbers, Real and Imaginary Parts of a Complex Number, Modules and Argument ( or amplitude) of a Complex number, Complex Conjugate, Square root of complex number, cube root of unity. De-Moivre's Theorem and its problems. Function and Limits concept or real function, types of functions, Composite function, domain and range of function, concept of limit, Standard Limits, Existence of Limits and Problesm Covered. Sketching of Curves ­ symmetry, tracing or Cartesian curves, asymptotes, and problems. Differentiation ­ Differentiation of the function, differentiation of trigonometric, inverse trigonometry logramithmic, exponential and function of function and problems. Application of Differentiation: - Tangents ad Normal Maxima and Minima, increasing and decreasing function, simple application ( in finding rate ) with their problems. Application ­ Area (quandrature) Volume and surface revolution, Rectification SEMESTER I BSCBI-105: Introductory Biology Unit I Living World Biology & Its Branches; relationships with other sciences; scientific methods in Biology; historical breakthroughs; scope of biology and career options; role of Biology in dispelling myths and misbelieves; characters of living organisms, (elementary idea of metabolism, transfer of energy at molecular level, open and closed systems, omoeostasis, growth and reproduction, adaptation, survival, death). Origin and evolution of life - theories of evolution; evidence of evolution; sources of variations ( mutation, recombination, genetic drift, migration, natural selection); concept of species; specification and isolation (geographical and reproductive); origin of species. Unit II Diversity of Life Variety of living organisms, Systematic; need, history and types of classification (artificial, natural, polygenetic); biosystematics; binomial nomenclature; Two kingdom system, Five kingdom System, their merits and demerits, status of bacteria and virus; botanical gardens and herbia; zoological parks and museums. Unit III Cell and Cell Division Cell as a basic unit of life - discovery of cell, cell theory, cell as a self - contained unit; procaryotic and eukaryotic cell; unicellular and multicellular organisms; tools and techniques ( PTU/DEP/Paramedical/September 2005/Detailed Syllabus Paramedical Courses Syllabus: 76 / 140 compound microscope, electron microscope and cell fractionation); Ultrastructure of prokaryoytic and eukaryotic cell - cell wall, cell membrane - unit membrane concept (flauid mosaic model); membrane transport; cellular movement (exocytosis, endocytosis); cell organelles and their functions - nucleus, mitochondria, plastids, endoplamasic reticulum, Gogli complex, lysosomes, lysosomes, microtubules, centriole, vacuole, cytoskeleton, cilia and flagella, ribosomes. Molecules of cell; inorganic and organic materials - water, salt, mineral ions, carbohydrates, lipids, amino acids, proteins, nucleotides, nycleic acids (DNA and RNA); Enzymes (Properties, chemical nature and mechanism of action); vitamins, hormones and steroids. Unit IV Genetics Continuity of life - heredity, variation; mendel's laws of inheritance, chromosomal basis of inheritance; other patterns of inheritance - incomplete dominance, multiple allelism, quantitative inheritance.

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Chromosomes - bacterial cell and eukaryotic cell; parallelism between genes and chromosomes; genome, linkage and crossing over; gene mapping; recombination; sex chromosomes; sex determination; sex linked inheritance; mutation and chromosomal aberrations; Human genetics - methods of study, genetic disorders. DNA as a genetic material - its structure and repliaction; structure of RNA and its role in protein synthesis; Gene expression - transcription and translation in prokaryotes and eukaryotes; regulation of gene expression, induction and repression - housekeeping genes; nuclear basis of differentiation and development; oncoenes. Basics of Recombinant DNA technolgy; cloning; gene bank; DNA fingerprinting; genomics principles and applications, transgenic plants, animals and microbes.

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

Unit V Morphology of Plants and Animals Morphology - root, stem and leaf, their sturucture and modification; Inflorescence, flower, fruit, seed and their types;Description of Poaceae, Liliaceae, Fabaceae, Solanaceae, Brassicaceae and Asteraceae. Morphology of animals - salient features of earthworm, cockroach and rat; tissue systems, structure and function of tissues - epithelial, connective, muscular and nervious. Practical 1. Study of parts of Compound Microsope 2. Study of mitosis in onion root tip and animal cell (grasshopper) 3. Study of meiosis in onion flower buds, and testis iof grasshopper. 4. Study of cyclosis in leaf cell of Hydrilla, or Tradescantia and in Paramoecium. 5. Study of cell wall components ( cellulose, lignin, suberin and mucilage). 6. Study of mitochondria by staining with a Janus Green. 7. Study of specimens and their identification with reason - Bacteria, Oscillator, Spirogyra, Rhizopus, mushroom/braket fungi, yeast, liverwort, moss, fern, Pinus, one monocotyledon, one dicotyledon and lichens. 8. Study of characters of specimens and identification with reason - Amoeba, Hydra, Liver - Fluke, Ascaris, Leech, Earthworm, Prwen, Silk moth honey bee, snail, Starfish, Dogfish, Rohu, Frog, Lizards, Pigeon/ any other bird and rabbit/ any other mammal. 9. Study of squamous epithelium, muscle fibres, nerve cells and mammalian blood film through temporary/permanent slides. 10. Study of external morphology of earthworm, cocroach, from and rat through models. SEMESTER I BSCBI-106: Communication & Soft Skills UNIT I Essentials of Grammar: · Parts of Speech · Punctuation · Vocabulary Building · Phonetics UNIT II Office Management: · Types of Correspondence · Receipt and Dispatch of Mail · Filing Systems · Classification of Mail. · Role & Function of Correspondence · MIS · Managing Computer UNIT III Letter & Resume Writing: · Types of Letters-Formal / Informal · Importance and Function · Drafting the Applications · Elements of Structure · Preparing the Resume · Do's & Don'ts of Resume · Helpful Hints UNIT IV Presentation Skills: · Importance of Presentation Skills · Capturing Data

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· Voice & Picture Integration · Guidelines to make Presentation Interesting · Body Language · Voice Modulation · Audience Awareness · Presentation Plan · Visual Aids · Forms of Layout · Styles of Presentation. UNIT V Interview Preparation: PTU/DEP/Paramedical/September 2005/Detailed Syllabus Paramedical Courses Syllabus: 78 / 140 · Types of Interview · Preparing for the Interviews · Attending the Interview · Interview Process · Employers Expectations · General Etiquette · Dressing Sense · Postures & Gestures UNIT VI Group Discussion & Presentation: · Definition · Process · Guidelines · Helpful Expressions · Evaluation (Note: Every student shall be given 15 minutes. of presentation time & 45 minutes of discussion on his/ her presentation.) The student will be evaluated on the basis of : his / her presentation style Feedback of Faculty & Students General Etiquette Proficiency in Letter Drafting / Interview Preparation The paper is internal and at least 3 tests will be taken. Best 2 of 3 shall account for final grades (70% Test & 30% Presentation) SEMESTER I BSCBI-107: Practical Unit - I Practical Inorganic Chemistry Wet Test Analysis for Basic Radical:i) Analysis of Zero Group ­ NH4+ ii) Analysis of Group I ­ Pb++, AG+ iii) Analysis of Group II ­ PB++BI++, CU++, AS++ iv) Analysis of Group III ­ Fe3+, AL3+, CR3+ v) Analysis of Group IV - ZN++, Mn++, Ni++, CO++ vi) Analysis of Group V ­ Ba++, Sr++, Ca++ vii) Analysis of Group VI ­ Mg++ Wet Test for Acid Radicals :Co3---, Sc3---, S--, NO2-, CH3COO--, Cl-, Br-, I-, NO3-, SO4-- Unit ­ II Volumetric Analysis i) Acidimetry & Alkalimetry ii) Oxidn ­ Rednn Titration. Unit ­ III Quantitative Analysis of Organic Compound Carboxylic Acid, Aldehyde, Ketones, Alcohol, Phenol, Amine & Amides. Unit ­ IV C++ Practicals 1. Write a Programme (WAP) to convert temperature in Degree Celsius to Fahrenheit.

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2. WAP to find whether a person is eligible for voting or not. 3. WAP to find the roots of a quadratic equation 4. WAP to find the sum of the series like - Sum = 1-x+x^2/2! ­ X^3/3!+x^4/4!...... 5. WAP to find simple interest 6. WaP that computes the bionomial coefficients C for positive, whether n is greater than r. 7. WAP to find the number of vowels in a string. 8. WAP to find out wheter a given word is a palindrome or not. 9. Write a menu driven programme using switch statement to find the area of a triangle, rectangle and square. 10. WAP to find the factorial of a given number 11. WAP to find the smallest and largest element of an array 12. WAP to sort the elements of an array by bubble sort method. 13. WAP to sort the elements of an array by selection sort method. 14. WAP to carry out linear search in an array 15. WAP to carry out binary search in an array 16. WAP to convert a decimal number into its binary equivalent 17. WAP using functions, that reverses an integer number and finds the sum of the individual digit. 18. WAP to swap two values by call by reference and call by value method 19. WAP for matrix addition 20. WAP for matrix multiplication 21. WAP to do diagonal exchange in a two dimensional array 22. WAP to implement stack operation PUSH & POP. 23. WAP to implement Queue Operation - Insertion & Deletion.

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SEMESTER II BSCBI-201: Biostatistics UNIT I : Introduction to statistics Definition, Descriptive & inferential statistics, Data & Its Collection, Classification of data, Importance & usefulness of statistics, limitation of statistics. UNIT ­ II : Manipulating the numbers Logs & exponents , Areas & volumes, Units & conversion UNIT ­ III: Measures of Central Tendency :- Mean, Mode, Median, Variability ­ Range, Quartile, Mean Deviation, Standard Deviation Probability ­ Statistical & Classical definition, Multiplicative & Additive Law of Probalility. UNIT ­ IV: Test of Significance: Chi-squared test, Regression methods, one and two way analysis of variance. UNIT ­ V: Bio assay, Factorial Analysis, Fisher exact test. SEMESTER II BSCBI-201: ORGANIZATION OF FUNCTION OF CELLS AND ORGANISATION. Unit I: Cells and their molecular organization: Cell as a basic unit of life: Cell theory, types of cells, fine structural details of cell ­ cell wall, cel organelles & their function. Unit II: Methods for studying cells Microscopy ­ light, phase contrast, electron; staining technique; microtomy. Unit III: Activities of Cells: Enzymes, energy production, energy transducers, photosynthesis & harvesting of energy, energy production in anaerobes & aerobes, TCA cycle. UNIT IV: Cell cycle: Mitosis, protein regulation cell cycle, DNA replications, Transcription Translation in prokaryotes & Eukaryotes; Regulation or protein synthesis in prokaryote & eukaryotes. UNIT V : Mammalian Histology: Cell differentiation in unicellular & Multicellular organisms; cell motility & contractile mechanism ­ amoedoid, celliary & glagellar Motion, Muscle contraction. PTU/DEP/Paramedical/September 2005/Detailed Syllabus Paramedical Courses Syllabus: 81 / 140

SEMESTER II BSCBI-203: FUNDAMENTAL & PRINCIPLES OF CHEMISTRY. UNIT 1 Second Law of thermodynamics: Carnot cycle, Concept of entropy and derivation using carnot cycle, Entropy changes in reversible & irreversible reactions. Electrochemistry ­ Nernest theory, electrical potential, nernets equation, Electrochemical series and its uses. Calomel electrodes, Gas electrodes, Redox electrode, Voltic Cell or Galvanic Cell, EMF ­ Concept & Measurement. Unit ­ II Chemical Kinetics: Reaction Velocity for 1st , 2nd and Zeor order reaction, Archeries equation, activation energy. Unit ­ III Coordination Chemistry : Werner's theory, Application of Werner's theory, coordination number, IUPAC nomenclature, Nomenclature of Organic compounds.

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Spectroscopy: Basis of U.V. ­ Vis.. & I.R. spectroscopy, Application to structure determination of organic compounds.

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

Unit ­ IV; General chemistry for elements of Periodic Table Group I, II, III, IV elements. Biomolecules : Carbohydrates ­ Mono, di and polysaccharides, their structure and classification and chemical properties. Amino & Proteins: Aminoacids and their classification, peptides, methods of synthesis of amino acids and peptides, determination of structure of polypeptides and group analysis. Proteins ­ general account. Primary and Secondary Structure of Proteins. Unit - V: Chromatography : General consideration, principle absorption, paper, thin layer & ion exchange chromatography. Analysis of soaps, detergents, cleaning agents, pesticides, using chromatographic methods, Analysis of water ­ deterioration alkalinity, dissolved )2, BOD, COD, Hardness, Chlorides and Phosphates. SEMESTER II BSCBI-204: Biochemistry Unit I Carbohydrates: Introduction, Classification Strucutre, Occurrence and biological importance of monosaccharides, general properties of carbohydrates with reference to glucose: Stereoisomerisms, D and L series, optical activity, cyclic structure, mutarotation, chemical reactions of monosaccharides structure, occurance of oligosaccharides and polysaccharides e.f. cellulose, glycogen starch, insulin, chitin, pectin's protoglycans, Unit II: Lipids Definition & Classification of fatty acids, properties of saturated and unsaturated fatty acids. Essential fatty acids. Specification number, acid number, Reichrt ­ Meissel number, iodine number, rancidity of fats, Chemistry and function of phospholipids (Lecithin, Phosphatidyl, Serine, cephalins, plasmalogens), sphingomyelins, glycolipids (cerebrosides, gangliorides). Unit III: Proteins Introduction, classification based on shape and size. Solubility and Functioned Properties. Classification and strucuture of amino acids. Essential Amino Acids. Properties of Amino Acids, Optical Isomerism, Amphotreric nature, is electric, point, titration of amino acids, chemical properties. Primary, Secondary ( alpha helix and bets- pleated sheet), tertiary structure of proteins, forces stabilizing the tertiary structure and quaternary structure of proteins structure and Biological functions of fibrous proteins ( keratins, collagen and elastin ). Unit ­ IV Nucleic Acid Structural plan of nucleic acids ( nucleosides, nucleotides) and their nomenclature, composition of DNA and RNA, feature of DNA, double helix. Structure and role of different types of RNA. Unit ­ V: Enzyme Basic Concept Classification, Nomenclature, Mechanism of enzyme action, basis of enzyme kinetics, Michaelis ­ Menten constant, Enzyme Inhibitors, Regulation of enzyme action. SEMESTER II BSCBI-205: Practical Biostatics & ORGANIZATION OF FUNCTION OF CELLS AND ORGANISATION. 1. To solve numerical based n Mean, Mode and Median. 2. To solve numerical based on Probability. 3. To test hypothesis by Chi-Square, Regression Method. 4. Numerical s based on factorial analysis 5. To study living organisms in from water samples. 6. To perform simple staining techniques 7. to perform flagella, chromosome, nucleus,

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8. Cell wall staining of the cells 9. to study photosynthetic activity in green plant. 10. to extract and study the absorption maximal of chlophyll pigment. FUNDAMENTAL & PRINCIPLES OF CHEMISTRY. & Biochemistry 1. To perform the qualitative analysis of Carbohydrate. 2. To perform the qualitative analysis of Lipids. 3. To perform the qualitative analysis of Proteins. 4. To perform the quantitative analysis of Carbohydrates by an throne nd DNS method. 5. To perform the quantitative analysis of Proteins By Biruet & Lowry's Method. 6. To determine acidic values of fats PTU/DEP/Paramedical/September 2005/Detailed Syllabus Paramedical Courses Syllabus: 83 / 140 7. To estimate the concentration of nucleic acids by spectrophotometric method. 8. Separation of amino acids by one and two dimensional paper chromatography. 9. Separation of Dyes by thin layer chromatography. 10. Determination of PH of given solution by glares electrodes. 11. Analysis of New Group, Group I, II, III IV elements. 12. To determine the COD, BOD of given water sample. 13. To perform experiments to remove temporary hardness of water. PTU/DEP/Paramedical/September 2005/Detailed Syllabus Paramedical Courses Syllabus: 84 / 140

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SEMESTER III BSCBI-301: HUMAN BIOLOGY AND EVALUATION. Unit I : Human Genetics : Human Chromosomes Determination of sex, sex linked inheritance, chromosal aberrations; inborn error of metabolism , gene transfer in mammalian cells. Unit II : Developmental Genetics: Factors controlling development; changes in development ( nuclear transplantation ). Turning genes on & off during development: Gene Post & Gene Frequencies. Hardy We in Berg Law. Unit III: Gene Constancy & Variability: Cell Division, Menedelian Segregation & Assortment, Dominance relation & Multiple Alleles Sex Linkage & Recombination Chromosomal number & Structure. Unit IV: Ecological Aspects : Some ecological aspects of population growth, Genetic Load and Genetic Death. Coss of Evolution Neutralist, Augment Selections, Augument Adative peak, Group Selection, Coevolution. Unit V: Genetic Basis of Evolution & Speciation: Theories of Organic evolution (Lamarckism & New Lamarckism; Darwinism) Evolution at mole cedar level ( nucleotides, Sequence and amino acid sequence molecular evolution); speciation ( races, species & modes of speciation). SEMESTER III BSCBI-302: Discrete and Numerical Mathematics Unit I: Discrete Mathematics: Statements and logical connections, Tautologies and contradictions. Logical equivalence. Algebra of proportions. Boolean algebra and its properties. Algebra of propositions partial order relations, Algebra of electric circuits and itsl applications. Design of simple automatic control system, Boolean function, Binary operations, Functions and Algorithms, Logic and Propositional calculus. Unit II: Numerical Mathematics: Solution of algebric and transcendental equations. The Bisection method. The iteration method, method of false position. Raphson method solution of linear systems. Direct method, matrix iterative method. Gausssion Elimination method. Iterative method, numerical differentiation and integration, numerical solutions of ordinary differential equations, Picard's method od successive Approximation. Euler's method, numerical solutions of differential equations.

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SEMESTER III BSCBI-303: Metabolism Unit I : Digestion and absorption of carbohydrates. Glycolysis, Glycogenesis, Glycogenolysis, kerb cycle, Pentos phosphate pathway, substrate level phosphorylation, gluconeogenesis. Regulation of carbohydrate metabolism. Unit II : Digestion and absorption of fats. Biosynthesis and degradation of fatty acids. Phospholipids, ketone bodies, regulation of lipid metabolism. Unit III : Digestion & Absorption of Proteins. General reaction of protein metabolism:- Transamination, DEamination, Urea Cycle, Metabolism of amino acids, Ketogenic and glucogenic amino acids. Unit IV : Biosynthesis & degradation of purines and pyrimidines and their regulation. Biological role of mineral and trace elements. Metabolism of mineral ­ Na, K, P, Cl, S and Ca. Unit V: Energy Metabolism:Free energy, endergonic and exergonic reaction, caloric value of good stuffs and its calculation. Basal metabolism and BMR, factors influencing BMR. Respiratory Quotient (RQ) and various factors affecting RQ. SEMESTER III BSCBI-304: Calculus, Vector Calculus Linear Algebra. Unit I : Calculus ­ Successive differentiation, Taylor's series, Differential equations ­ Equations of the first order and first degree, equations in which the variables are seperable, homogeneous equations, equations reducible to a homogeneous form, linear equations and reducible to the linear form, exact differential equations, change of variables. Unit II : Vectors Addition & Subtraction of vectors. Types of vectors, Unit vectors ( Two & Three dimensional), scaler product of two vectors, vector product of two vectors. Scalar triple product. Vector triple product. Vector differentiation and integration, rate of change in a given direction, flux of a vector field through a surface. Unit III : Laplace transforms, application of Lapalace transforms for solving constant coefficient ordinary differential equations with discontinuous forcing terms. Unit IV: Linear Algebra ­ Vector spaces, subspaces and algebra of subspaces, Linear combinations of Vectors and span of a set, linear dependence and independence of vectors. Eigen values and Eigen vectors of matrix. Unit V: Matrices and Simultaneous equations. PTU/DEP/Paramedical/September 2005/Detailed Syllabus Paramedical Courses Syllabus: 86 / 140

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SEMESTER IV BSCBI-401: Molecular Genetics Unit I : DNA & THE MOLECULAR STRUCTURE OF CHROMOSOMES: Function of genetic material ; structure of DNA & RNA, chromosome structure in Prokaryotes, Eukaryotic Genomes, Repeated DNA sequences. Unit II : Replication of DNA & Chromosomes Basic features of replication. Enzymes involved in replication. Complex replication apparatus: Eukaryotic chromosome replication cell cycle. DNA polymerizes. Duplication of nucleosome. Unit III: Gene expression and its regulation Central Dogma, Transcription processing, MRNA, Genetic code: Translation: Eukaryotic control of gene expression. Unit IV : Variation in structure of chromosomes Inversion, Translocation, Phenotypes, effect of chromosomes, Rearrangement. Linkage crossing over & chromosomes mapping ­ linkage crossing over as physical basis recombination, chiasmata formation : gene mapping Unit V : Recombinant DNA technology Vectors, Restriction enzymes, methods of insertion of foreign DNA into vector; Transformation, screening & selection of recombinant clones: libraries, DNA sequencing, PCR. DNA finger printing. SEMESTER IV BSCBI-402: Object Oriented Programming Using C++ Unit I : Principles of OOP Software crisis. Software evolution. A look at procedure oriented programming. Object oriented programming paradigm. Basic concepts of OOP, benefits of OOP. A Simple C++ program. C++ Statements. An example with class. Structure of a C++ programme. Creating Source File. Compiling and Linking. Token, Expressions and Control Structures : Introduction , Tokens, keywords, identifiers, Basic data types, Defined data types, Derived data types, Symbolic constants, Type compatibility, Declaration of variables, Dynamic initialization of variables, Reference variables, Operators in C++, Scope resolution, Operator, Memeory management Operators, Manipulators, Type Cast Operator, Expression and Implicit Conversions, Operator Overloading, Control structures. Functions in C++ The Main function. Function prototyping, Call by references. Inline Function, Default arguments, Const Arguments, Function overloading, Friend & Virtual functions. UNIT ­ II Classes & Objects: C structures revisted, Specifying A Class. Defining Member Functions, Making an Outside Function Inline. Nesting of Member Functions, Private Member Functions, Arrays & Class, Memory Allocation for Objects, Static and Data members, Static Member Functions, Arrays of Objects, Objects As Function Arguments. UNIT - III Constructors and Destructors: Introduction, Constructors, Parameterized constructors, multiple constructors in a class, Constructors with default arguments, Dynamic initialization of objects, copy constructors, dynamic constructors, Constructing two dimensional arrays. Destructors. Operator Overloadings & Type Conversions: Introduction, defining operator overloading, Unary operator, Overloading binary operator, Using Friends, Manipulation of Strings using Operators, Rules for Overloading Operators, Type conversions. UNIT ­ IV Inheritance Extending classes : Introduction, Defining derived classes, Single inheritance, making a private member inheritable, multiple level inheritance, hierarchal inheritance, hybrid inheritance. UNIT ­ V Pointers, Virtual Functions and Polymorphism: Introduction, Pointer operations, to Objects, This pointer. Pointers to derived classes, virtual functions, pure virtual functions, managing console I/O Operations, C++ Streams, C++ Stream Classes. Unformatted I/O Operations. Formatted Console I/O Managing Output With Manipulators.

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SEMESTER IV BSCBI-403: Management of Biological Data base Unit I: Introduction Purpose of database system. View of data, Data models, database languages, Databse administrator; database users, transaction management Entity relationship model : Basic concepts, Design issues, Mapping constraints, E-R diagrams, Weak Entity Sets, Extended E-R features. Unit II Relational Model : Structure of relational database. The relational algebra, Extended relational algebra operations, Basic SQL programming using above algebra concepts. Unit III Relational Database Design: Normalization and Integrity Constraints, Database Applications, Decision Support Systems, Databse and WWW, Datat Warehousing, Spatial & Geographic database, multimedia database. Unit IV Network Model: Basic concepts, Data Structure Diagram. The DBTG CODASYL model. Implementation techniques. Hierarchical Model : Basic concepts, Tree structure diagrams, Implementation techniques. The IMS database system. Unit V : Developer 2000 : Application development in Forms form, Menu, Library, Form module ­ Items, Canvas View, Windows. Using forms designer, creating a form. Generating & running a form. Using the layout editer. Constraints defined at the time of creation of a block. Triggers ­ Interface events, internal processing events, Sequence of trigger execution, writing triggers. Behaviour of Oracle form in Commercial Applications. Working with reports ­ Features, Data Model, Master Detail Reports.

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SEMESTER IV BSCBI-404: Artificial Intelligence Unit I : Overview of artificial intelligence: What is AI, Importance of AI, Early works in AI & Related Fields. Knowledge : General Concepts ­ Introduction, Definition and Importance of Knowledge. Knowledge Based Systems. Representation of knowledge. Knowledge Organization, Knowledge Manipulations. Acquisition of Knowledge.

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

Unit II : Formalized symbolic Logics ­ Syntax and Semantics for prepositional logic, syntax & semantics for FOPL, Properties of WFF's conversion to clausal form. Inference rules, the resolution Principle, Non deductive Inference Method, Representation using Rules. Truth maintenance system, default reasoning & the closed world assumption, predicate Completion and circumscription, model & temporal logics, fuzzy logic and natural language computations. Unit III: Structured Knowledge : Association networks, Frame structure, Conceptual dependencies and scripts. Object oriented representation. ­ Overview of object oriented language and systems. Unit IV: Search & Control Strategies - Preliminary Concepts, Examples of search problems, Uninformed or Blind Search, Informed Search , searching AND-OR graphs. Matching Techniques: Structures used in matching, measure for matching , matching like patterns, partial matching, fuzzy matching. Algorithms, RETE Matching Algorithm. Knowledge Organization and Management ­ Indexing & Retrieval Techniques, Integrating Knowledge in Memory, Memory Organization Systems. Unit V: Natural Language Processing Overview of linguists. Grammars & Languages, Basic Parsing Techniques, Semantics Analysis and Representation structures, Natural Language Generation. Natural Language Systems. Pattern Recognition: The recognition and classification process, learning classification patterns, Recognizing and Understanding speech. SEMESTER IV BSCBI-405: Practical Molecular Genetics & Object Oriented Programming Using C++ 1) Isolation of Chromosone from Chryonemous Larvae. 2) Isolation of Chromosomal & plasmid DNA from bacteria PTU/DEP/Paramedical/September 2005/Detailed Syllabus Paramedical Courses Syllabus: 89 / 140 3) To study mitosis & meosis process in onion root tips. 4) To study the effect of mutation on bacteria by U.V. treatment 5) To isolate antibiotic resistant auxotrophic mutants. 6) Define a class STRING, use Overloaded ­ operator to compare teo strings. 7) Define a class to represent a bank account. Include te following data members and member function Data Members. i. Name of depositor ii. Account number iii. Type of account iv. Balance amount in account Member Function 1) To assign initial values 2) To Deposit on amount 3) To withdraw an amount after checking the balance 4) To display name & balance Write a main program to test the program 8) Write a program to prepare an invoice from the following data. Customer no, Customer name, address., date of sale, item no, item description, quantity sold, unit price of item, discount, percentage, sales tax percentage. Note: Identify different classes possible here and make sure that the date of sale becomes equal to today's dates as soon as the object is created. Today's date should be accepted from the user in beginning.

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9) Write a C++ program to perform various operations on string class without using the inbuilt functions, operations on a class are : 1) Read a string 2) Display a string 3) Reverse a string 4) Copy the string into an empty string. 5) Concentrate two strings. 10) Write a program in C++ o generate mark sheet of 10 students in a proper formal format. PTU/DEP/Paramedical/September 2005/Detailed Syllabus Paramedical Courses Syllabus: 90 / 140

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SEMESTER V BSCBI-501: Immunology Unit 1: Overview of Immunology: - Immunity, types of immunity, Lymphoid organs & cells. Antigens & Antibodies ­ structure, classes & function. Unit 2: Immune Response: - B-Cells, T-Cells, Activation of B-Cells & T-Cells. Initial Interactions of Ag, Generation of antibody diversity, lymphokines, their mode of action, response of activated T-cells, Primary and Seconday immune response. Unit 3: Immunochemistry ­ Antigen ­ Antibody reaction, Pecipitation, Agglutination, Neutralization, Complement fixation, ELISA, monoclonal antibody production, Immuno-electrophoresis, immunoassay methods. Unit 4: Cell response to stress:- Role of proteases and molecular chaperones, signaling pathways, apodisis, growth control; cancer Unit 5: Immunobiotechnology:- Monoclonal antibody production, animal cell culture; transfenic animals, Plant Biotechnology ­ transgenic plants, fruit ripening and production of insect resistant plants. SEMESTER V BSCBI-502: Biomark up Language and System Programming Unit I: Advance C with Pointers. Introduction to pointers ­ The & and * operator, Pointer expressions. The Jargon of Pointers, Char, INt , Float pointers. Passing address to functions, functions returning to pointers, pointers in one dimension and two dimensions. Unit II: Unix preview: - The Kernel, Running a command- The shell, files and Directories, Peripheral devieces and UNIX: special files. Working with Bourne Shell.: filename Expansion, shell meta characters, shell variables, shell scripts, shell command. The Enviornment, Basic Unix command,s Ls, cat, cmp, more, diff, chmod, chown, tee, sort, rm, md, mv, who, whoami, banner. Unit III: Shell Scripts: - The for loop. The case statement conditional looping with while & Until. The ifstament, the test command, Error Checking, Adding Arithmatics to a shell script: expr. Unit IV: Web Pages & Html: An overview of HTML ­ Viewing HTML pages, HTML elements and tags, essential elements of web page. Adding structure to a page. Using prargraphs on line breaks, Dividing sections with horizontal lines, creating a hierarchy with headings. Formatting Text and Pages ­ Formatting Text, Formatting Pages Using style and style sheets. Unit V: - Linking pages to the world ­ Creating a test or Image hyperlink, Specifying other link targets. Including pictures in a page, working with Tables, Using font style elements. Forms Designing, Creating a Feedback Form. SEMESTER V BSCBI-503: Programming Project The programming project focuses on developing student's skills sdesign, implementation, testing and documentation, Students learn to design for portability and reuse, as well as to take advantage of system utilities and standard libraries. The assignments require students to work in small programming teams and result in the development of a small-scale software system. The project assumes good programming skills in C++ and working knowledge of Windows programming and integrates previously learned skills and knowledge relating to social and ethical issues.

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PTU/DEP/Paramedical/September 2005/Detailed Syllabus

SEMESTER V BSCBI-504: Partial Differential Equation Unit I :- Partial differential equation or first order, Lagrange's linear Partial Differential Equation. Lagrange's solution, Lagrange's method of solving the linear partial differential equation of order one of the type Pp+Qq = R, Charpit's method. Unit II:- Partial differential equation of second and higher orders. Classification of linear partial differential equations of second order. Homogeneous and non-homogeneous equations with constant coefficients. Partial differential equations reducible to equation with constant coefficients. SEMESTER V BSCBI-505: Practical Practical based on Immunology & Biomark up Language and System Programming 1) Perform blood grouping of individual. 2) To study precipitation reaction by Outcherloney double diffusion method. 3) To perform immunoelectrophresis 4) To perform elisa test 5) SQL Programming Tables CUS Cust-id, lname, fname, area, phone-no MOVIE Mv-no, Title, Type, Star, Price INV-SYS Inv-No, Mv-no, Cust-id, Issue-date, Ration-date Queries ­ 1) Fin the name of all the customers that begin with s or a 2) Print the list of employees whose phone-number are greater than 555000 3) List the movies in the sorted order of their titles 4) Determine max, min movie prices and rename their titles. 5) Print the type and average price of each movie. 6) Calculate the average price of all the movies where type is comedy or thriller and price ­ 150 7) Find out the names of customers who have been issued movies of type drame 8) Find out which customers have been issued movie number = 9. Project Viva of Programming Project.

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PTU/DEP/Paramedical/September 2005/Detailed Syllabus

SEMESTER VI BSCBI-601: Microbiology & Virology Unit I : History and Contribution of Poneers: Microbes and friends and Foes. Microbial diversity, Cassification of microbes: classification methods, classification of bacteria, viruses & fungi. Unit II: Ultra structure & Five structure of bacteria cell wall:- Structures external to cell wall flagella pill, capsule; structures internal to cell wall ­ cell. Membrane, ribosomes, culear material etc... Unit III: Microbial Growth: Growth Curves: Lag phase, exponential phase, stationary, transition period, arithmetic expression of growth, entwines continuous & synchronous culture; factors affecting growth. Unit IV: Virus: structure of virus- capsid: envelop, Nucleic acid, classification of viruses, plant, animal & bacterial viruses. Isolation purification and assay of viruses. Unit V: Multiplication of Viruses: Lytic cycle, Lysogenic cycle, adsorption, penetration replication and release, one step growth curve. Biotechnological importance of viruses, production of vaccine, biopesticides, interferons. SEMESTER VI BSCBI-602: Numerical Analysis Unit I: Representation of Numbers, Computer Arithmetic, Errors in Arithmetic, Errors in Arithmetic, Roots of Non Linear equation ­ Bisection method, false postion method, Newton Raphson method, fixed point method, muller method. Unit II: Matrices ­ Hermitian & Systematics matrices, Determinants & Inverse of A Matrix, Rank and Nullity of Matrics, Solution of Non-Homogeneous Liner equation by Cramer rule, characteristics Roots. Caley Hamiltion Theorem. Unit III: System on Linear Algebric Equations Direct Solution ­ Existence of solution. Basic gauss Elimination method, Trangular factorization method, matrix Inversion method. Iterative solution: Jacobi Iteration method, Gauss Siedal method. Convergence of Iteraton methods. Unit IV: Interpolation and Appropriation: Interpolation concepts, lagrange, Interpolation, Nweton Interpolation formula. Divided Difference Table, Interpolation with equidistant points - Forward difference, Backward difference. Unit V: Numerical Differentiation ­ Differentiating Tabulated Functions ­ Error analysis, Higher Order derivates. Numerical Integration - Newton cotes method, Trapezoidal rule ­ Error analysis, Composte Trapezoidal, Simpson Rule ­ Error Analysis. Composite Simpson Rule.

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PTU/DEP/Paramedical/September 2005/Detailed Syllabus

SEMESTER VI BSCBI-603 Data structure algorithms & Compliers Unit I : Introduction to Data structure The concept of Data Structure, Abstract Data Structure, Analysis of Algorithm, The Concept of List. Stacks and Queues: Introduction to Stack & Primitive Operation on Stack, Stack as an abstract data types, Multiple Stack, Stack Applications; Infix, Postfix, Prefix and Recursion, Introduction to Queues, Queue as an Abstract Data Type, Circular Queue, De Queue, Priority Queue. Unit ­ II Linked List: Introduction to Linked List of Stacks, The Linked List of Queue, Header Nodes, Doubly Linked List, Circular Linked List, Stacks and Queue as a Circular Linked List. Unit III Trees: Basic Terminology, Binary Trees, Tree Representation as Array & Linked List, Binary Tree Representations, Traversal of Binary Trees: In-Order , Preorder & Post Order. Application of Binary Trees. Threaded Binary Tree, B-Tree & Height Balanced Tree, Binary Tress Representation of Trees, Counting Binary Trees. Unit IV Searching & Sorting: Sequential Searching, Binary Searching, Insertion Sort, Selection Sort, Quick Sort,Bubble Sort, Heap Sort, Comparison of sorting methods. Tables & Graphs : Hash Table Collision Resolution Techniques, Introduction to Graphs, Definition, Terminology, Directed, Undirected and Weighted Graph. Representation of Graphs, Graph Traversals; Depth First & Breatch First Search, Spanning Treem Application of Graphs. Unit V Compilers & Translators, Why do we need Translators, Structure of Compilers, Error Handling, Book Keeping, Syntax Analysis, Lexical Analysis, Intermediate Code Generation. SEMESTER VI BSCBI-604 : Software Engineering The Software Engineering project enables students to experience real-life involvement, in the planning and development of a medium sized software project. It utilizes a teamwork approach t o software engineering and integrates previously learned skills and knowledge relating to social andethical issues. The subject covers project management skills development such as team organization, cooperation, communication, project planning, milestones presentation ad reporting. Implementation includes requirements and functional specification, analysis and design, testing, documentation and maintenance. SEMESTER VI BSCBI-605 : Practical Based on Microbiology & Virology 1) To isolate Microorganisms from soil by plant method. 2) To perform Gram staining, simple staining of Microorganisms. 3) To study the growth curve os bacteria by O.D., dry weight & Wet weight method. 4) To isolate plant viruses 5) To study various plant and animal viral diseases 6) To perform phage titration test. 7) To study one-step growth curve of bacteriophage, Project Viva based on Software Engineering.

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PTU/DEP/Paramedical/September 2005/Detailed Syllabus

M.Sc. (Medical Lab Technology) (MSCMLT)

SEMESTER I

MSCMLT-101: Human Anatomy & Physiology

Unit I Cell structure, division & function Cell organelles Tissue: Types of tissues and their functions Skeletal system Unit II Digestive system: Physiology and anatomy of mouth, stomach, intestine Absorption of food and its excretion Role of Bile in digestion and excretion Liver function and a brief description of liver and biliary tree Unit III Respiratory system: Brief description of larynx, bronchi, lungs Cardiovascular system: Anatomy and Physiology of heart, arteries and veins Circulation: Systematic and pulmonary (in brief) Brief review of chambers Unit IV Urinary system: Structure and Function of the Kidney, utrus, bladder, urethra and nephron Give special emphasis on formation of Urine Physiology and Anatomy of male and female reproductive organs Endocrine: Pituitary, thyroid, parathyroid, thymus, adrenals and pancreas Unit V Central nervous system: Brain, spinal cord and meninges explain with its functions Skins: Structure and Functions Study and give small project on bones and cartilages HLA system

SEMESTER I

MSCMLT-102: Basic Biochemistry

Bioenergetics, Entropy, Enthalpy & their basic introduction Concept of free energy, Thermodynamics 1st & 2nd Law. Carbohydrate: Structure, properties,, chemical reactions & functions Amino Acids: Essential & non Essential amino acids with structure & function Proteins: Primary, Secondary, tertiary & quatnery (Overrview) Lipids: Structure, Classification & properties Enzymes: Classification, enzyme action & their mechanism. Enzyme inhibition, Mode of action of chymotrypsin & related enzymes Nucleic Acids: Structure of Purine & pyrimidine bases Nucleotide & Nucleosides DNA & RNA: Structure & Properties Vitamins

SEMESTER I

MSCMLT-103: Clinical Biochemistry

Unit - I Carbohydrates: Carbohydrates intermediate metabolism, glycogenesis, glycogenolysis, gluconeogenesis & glycolysis. TCA, HMP, and its regulations Disorcerds of carbohydrates metabolism related to each cycle (inborn error of metabolism) Unit ­ II Proteins: Different metabolic pathway of amino acid The flow sheet of amino acids oxidation. Transamination, oxidativedeamination and pathways leading to acetyl co-A. Decarboxylation of Amino acids, formation of nitrogenous excretion products. Urea cycle and ammonia excretion. Unit ­ III Lipid: Biosynthesis and oxidation of fatty acids (odd & even number ) Ketone bodies formation and their oxidation Regulation and inborn error of lipid metabolism

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PTU/DEP/Paramedical/September 2005/Detailed Syllabus

Unit ­ IV Biochemical aspects of Hormone: Hormone receptors and intracellular messengers, Adenylate cyclase, protein kinase and phosphodiesterase. Role of Insulin, glucagons, epinephrine and their mechanism Various endocrine and regulatory systems mediated by cyclic AMP. Unit ­ V Vitamin: Fat and Water soluble and their deficiency Mineral metabolism : Minor and Major (cu, Fe, Ca, Mg & P) Inborn error of Nucleic acids metabolism Practical: Estimation of Protein by Folin's method in a given sample. Estimation of Glucose / GOD ­ POD method Estimation of bilirubin by kit method in a given sample Estimation of Urea by kit method in a blood / Urine Total protein test ­ A:G ratio Urine Analysis Chemical, Physical, Microscopical Draw a standard graph of GTT curve. Demonstration of electrophoresis Estimation of Sodium & Potassium by flame photometer.

SEMESTER I

MSCMLT-104: Basic Clinical Pathology

Introduction to Histology, the cell, cell Organelles, nucleus, cell division, tissues, fresh & fixed tissues. Different types of Embedding Viz. Wax, Resin, Cryostat etc. Basic Cytology Fixation of tissue, different kind of fixatives, sample fixative, compound fixative, formaldehyde, mercusic chloride, osmium, Picric acid, alcohols, other acids, formaline, buffered fomaline, osmic acid, zenleer soln, he;ly's soln, cytological fixatives, nuclear fixatives, fixation of smear etc., decalcification, method of decalcification, assessment of decalcification, soln for decalcification. Processing of tissue, dehydration, impregnation in the wax, manual and automatic tissue processor, gelatin embedding, celloidin embedding, double embedding, cytological fixatives, preparation of different smears, vaginal, sputum, membrane. Microtome, instrument, principle, use in section cutting, parts and working of commonly used microtome, different kinds of microtome, rotary, base sledge, sliding, low temperature microtome, cryostat, microtome knives, homing and stropping knives. Section cutting of paraffin sections, section preparation from frozen sections, fixing of tissue to slide, preparation of celloidin section and fixation. Staining techniques, natural dyes, synthetic dyes, basic and acidic dyes, haematoxylin staining, Pap, flucker & Conn, methanamine silver nitrate, ziehl neelsen's stain, propylene glycol sudan technique, papanicolaou, harn's alum, Haematoxylin, acridine orange technique.

SEMESTER I

MSCMLT-105: Introductory Biology

Unit I Living World Biology & Its Branches; relationships with other sciences; scientific methods in Biology; historical breakthroughs; scope of biology and career options; role of Biology in dispelling myths and misbelieves; characters of living organisms, (elementary idea of metabolism, transfer of energy at molecular level, open and closed systems, omoeostasis, growth and reproduction, adaptation, survival, death). Origin and evolution of life - theories of evolution; evidence of evolution; sources of variations ( mutation, recombination, genetic drift, migration, natural selection); concept of species; specification and isolation (geographical and reproductive); origin of species.

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Unit II Diversity of Life Variety of living organisms, Systematic; need, history and types of classification (artificial, natural, polygenetic); biosystematics; binomial nomenclature; Two kingdom system, Five kingdom System, their merits and demerits, status of bacteria and virus; botanical gardens and herbia; zoological parks and museums. Unit III Cell and Cell Division Cell as a basic unit of life - discovery of cell, cell theory, cell as a self - contained unit; procaryotic and eukaryotic cell; unicellular and multicellular organisms; tools and techniques ( compound microscope, electron microscope and cell fractionation); Ultrastructure of prokaryoytic and eukaryotic cell - cell wall, cell membrane - unit membrane concept (flauid mosaic model); membrane transport; cellular movement (exocytosis, endocytosis); cell organelles and their functions - nucleus, mitochondria, plastids, endoplamasic reticulum, Gogli complex, lysosomes, lysosomes, microtubules, centriole, vacuole, cytoskeleton, cilia and flagella, ribosomes. Molecules of cell; inorganic and organic materials - water, salt, mineral ions, carbohydrates, lipids, amino acids, proteins, nucleotides, nycleic acids (DNA and RNA); Enzymes (Properties, chemical nature and mechanism of action); vitamins, hormones and steroids. Unit IV Genetics Continuity of life - heredity, variation; mendel's laws of inheritance, chromosomal basis of inheritance; other patterns of inheritance - incomplete dominance, multiple allelism, quantitative inheritance. Chromosomes - bacterial cell and eukaryotic cell; parallelism between genes and chromosomes; genome, linkage and crossing over; gene mapping; recombination; sex chromosomes; sex determination; sex linked inheritance; mutation and chromosomal aberrations; Human genetics - methods of study, genetic disorders. DNA as a genetic material - its structure and repliaction; structure of RNA and its role in protein synthesis; Gene expression - transcription and translation in prokaryotes and eukaryotes; regulation of gene expression, induction and repression - housekeeping genes; nuclear basis of differentiation and development; oncoenes. Basics of Recombinant DNA technolgy; cloning; gene bank; DNA fingerprinting; genomics principles and applications, transgenic plants, animals and microbes. Unit V Morphology of Plants and Animals Morphology - root, stem and leaf, their sturucture and modification; Inflorescence, flower, fruit, seed and their types;Description of Poaceae, Liliaceae, Fabaceae, Solanaceae, Brassicaceae and Asteraceae. Morphology of animals - salient features of earthworm, cockroach and rat; tissue systems, structure and function of tissues - epithelial, connective, muscular and nervious. Practical 1. Study of parts of Compound Microsope 2. Study of mitosis in onion root tip and animal cell (grasshopper) 3. Study of meiosis in onion flower buds, and testis iof grasshopper. 4. Study of cyclosis in leaf cell of Hydrilla, or Tradescantia and in Paramoecium.

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PTU/DEP/Paramedical/September 2005/Detailed Syllabus

5. Study of cell wall components ( cellulose, lignin, suberin and mucilage). 6. Study of mitochondria by staining with a Janus Green.

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

7. Study of specimens and their identification with reason - Bacteria, Oscillator, Spirogyra, Rhizopus, mushroom/bracket fungi, yeast, liverwort, moss, fern, Pinus, one monocotyledon, one dicotyledon and lichens. 8. Study of characters of specimens and identification with reason - Amoeba, Hydra, Liver - Fluke, Ascaris, Leech, Earthworm, Prawn, Silk moth honey bee, snail, Starfish, Dogfish, Rohu, Frog, Lizards, Pigeon/ any other bird and rabbit/ any other mammal. 9. Study of squamous epithelium, muscle fibres, nerve cells and mammalian blood film through temporary/permanent slides. 10. Study of external morphology of earthworm, cockroach, from and rat through models.

SEMESTER I

MSCMLT-106: Communication & Soft Skills

UNIT I Essentials of Grammar: · · · · Parts of Speech Punctuation Vocabulary Building Phonetics

UNIT II

Office Management: · · · · · · · UNIT III Letter & Resume Writing: · · · · · · · UNIT IV Presentation Skills: · · · · Importance of Presentation Skills Capturing Data Voice & Picture Integration Guidelines to make Presentation Interesting

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Types of Correspondence Receipt and Dispatch of Mail Filing Systems Classification of Mail. Role & Function of Correspondence MIS Managing Computer

Types of Letters-Formal / Informal Importance and Function Drafting the Applications Elements of Structure Preparing the Resume Do's & Don'ts of Resume Helpful Hints

· · · · · · · UNIT V

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

Body Language Voice Modulation Audience Awareness Presentation Plan Visual Aids Forms of Layout Styles of Presentation.

Interview Preparation: · · · · · · · · UNIT VI Group Discussion & Presentation: · · · · · Definition Process Guidelines Helpful Expressions Evaluation Types of Interview Preparing for the Interviews Attending the Interview Interview Process Employers Expectations General Etiquette Dressing Sense Postures & Gestures

(Note: Every student shall be given 15 minutes. of presentation time & 45 minutes of discussion on his/ her presentation.) The student will be evaluated on the basis of : his / her presentation style Feedback of Faculty & Students General Etiquette Proficiency in Letter Drafting / Interview Preparation The paper is internal and at least 3 tests will be taken. Best 2 of 3 shall account for final grades (70% Test & 30% Presentation)

SEMESTER I

MSCMLT-107: Practical

Human Anatomy & Physiology Basic Biochemistry Clinical Biochemistry Basic Clinical Pathology Introductory Biology Internal Assessment - 15 Marks - 15 Marks - 15 Marks - 15 Marks - 15 Marks - 25 Marks

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SEMESTER II

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

MSCMLT-201: Instrumentation

Unit ­ I Mircoscope: Light, phase contrast, interference, fluorescence, polarization and electron microscopy (principle, parts and its application) Photometry: Basic principal UV-Vis spectrometry and colorimetry instrumentation and its application Unit ­ II Fluorimetry: Principal, Instrumentation and application Electrophoresis: Principal, types and application ( agrose gel electrophoreses, starch gel and polyacrylamide electrophoresis) Unit ­ III Centrifuge: Basic principle, type analytical and preparative centrifuges, different density gradient centrifuge and analytical with its application Blood analyzer: Principal, instrument and its application Unit ­ IV Microtome: Principal, instruments and its uses. Incubator, hot air oven and autoclave: Principal, instrument and its application. Unit ­ V Radioactivity: Radioisotopes, half life units, Geeger Mueller counter, gamma counter and scintillation PH meter (principle types, types of electrodes and application) Practical: Verification of Beer's Law Electrophoresis of protein ­ native and under denaturing condition. Separation of sub cellular organelles by different centrifugation Separation of blood cells by density gradient centrifugation Handling and management of instruments Estimation of blood urea creatinine uric acid, calcium and phosphorous

SEMESTER II

MSCMLT-202: Clinical Haematology

1 Red Blood Cells : a. Normal morphology count b. Isolation from whole blood & count c. Effect on count & morophology of physiochemical parameters & the diseased state d. Red cell anomalies & their relevance w.r.t normal & diseased state Blood Transfusion : a. Pre-requisitement & the complication of mis-matched transfusion b. Methods of blood matching White blood cells & platelets;a. Morphology count & methods of isolation b. Effect on count & morophology of cell by the physiochemical parameters , diseased . State & the relevance of condition of the diseases Anaemia's, : a. Definaation ( in general ) & courses b. Types of anaemia & their classification c. Physiochemical , characteristic features & eterology of a plastic anaemia, haemoloytic,megaloblastic d. linical features & diagonosis 5 Leukaemia a. Definition (in general ) & their etrology b. Classification of leukaemia c. FAB classification d. Etiologies ,physiochemical features of different Type of leukaeia,s with reference to clinical states e. Diagonosis of different types of leukaemias Coagulation studies ; a. General pathways ( intrinsic & extrinsic )

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b. Properties ( physiochemical ) mode of action of coagulation factors c. Platelet studies ,platelet function tests ( for different Coagulation factors ) > Effect of promoters & inhibitors at diff steps in coaguation,their solution & mode of action d. Diseases associated with coagulation disorders ,their etrology & characteristics features. Red Cell mass studies ; a. Chemical method & radioactive methods b. Red Cell function studies

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

SEMESTER II

MSCMLT-203: Blood Banking

Reception, labeling and recording of laboratory investigations Cleaning of glassware, pipettes, E.S.R tubes and counting chambers Preparation of capillary pipette, distilled water, reagents, buffers Collection of blood, preparation of blood smear, staining of blood and bone marrow smears. Measurement of hemoglobin, counting of leucocytes, erythrocytes, platelets and reticulocytes. Recognition of blood cells in peripheral blood smear Determination of haematocrite and E.S.R, preperation of haemolysate and determination of alkali resistant hemoglobin, paper electrophoresis of hemoglobin. Test for sickle celling, bleeding time, coagulation time, prothrombin time, and kaolin cephalin clotting time. Abo blood grouping and Rh typing Performance of direct and indirect coombs test, red cell agglutination test (screening Paul bunnel test). Preparation for the demonstration of L.E. Cell phenomenon. Blood donor selection & screening Blood collection and preservation, principal of clearing and preparing transfusion bottle and tubing sets ­ preparation and composition of anticoagulant ­ preservative solutions. Transfusion reaction and their investigations

SEMESTER II

MSCMLT-204: Lab Management

Ethics of the pathological clinics Organization of a pathology laboratory under board of quality control Personality development and patient relationship Pathology reports writing Computer application in pathological clinics Accountancy in clinical pathology Hospital Management Operation ethics Social ethics of pathology Proper handling of instruments

SEMESTER II

MSCMLT-205: Practical

Instrumentation Clinical Haematology Blood Banking Lab Management Internal Assessment : 20 Marks : 15 Marks : 20 Marks : 20 Marks : 25 Marks

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SEMESTER III

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

MSCMLT-301: Bacteriology

Unit ­ I Introduction, history and scope of microbiology Contribution of Anatomy Von Leeuwenhook, Louis Pasteur. Alexender Fleming in the development of Microbiology Morphology and ultrastructure of bacterial cell wall of eubacteria and archaebacteria cell membranes ­ structure, composition and properties Unit ­ II Bacterial Nutrition: Nutritional groups, common nutritional requirements, growth factors. Growth of bacteria under extreme conditions: Psychrophiles, thermophiles, halophiles and acidophiles Bacterial reproduction: Binary fission and endospore formation Unit ­ III Mycoplasmas: General characteristics, structure and reproduction Cyanobacteria: General characteristics, structure, reproduction and economic importance Bacterial growth curve, generation time, growth Kinetics ­ Synchronous, Batch and continuous cultures Measurement of growth and factors affecting growth. Unit ­ IV Chemical control of microorganims: Heat, Filtration and radiation Sterilization of soaps , detergents and dyes. Chemical control of microorganisms: halogens, phenol and phenolic compounds, heavy metals, alcohols, ethylene oxide, aldehydes and hydrogen peroxide. Unit ­ V Basis of microbial classification. Classification and salient features of bacteria according to Bergey's manual of determinative bacteriology. Kingdom fungi: Structure, reproduction and classification of fungi General characteristics and life cycle of : Zygomycetes, Ascmycetes Basidiomycetes and Deuteromycetes.

SEMESTER II

MSCMLT-302: Virology & Mycology

Unit ­ I General morphology and ultra structure of Viruses: Capsids- Helical Symmetry, icosahedral symmetry and complex symmetry. Envelope: Glycoprotein and matrix protein Viral genome: their types and structure Unit ­ II Cultivation of Viruses in embryonated eggs, experimental animals and cell culture: primary and secondary cell culture, suspension cell culture and monolayer cell cultures. Assays of viruses: physical and chemical methods of assays (protein nuclei acid, radioactivity traces, electrons microscopy, plaque method, pock counting method, end point method and infectivity of plant viruses). Serological methods: haemagglutination haemagglutinationinhibitation, complement fixation, immunofluroscence assays (IFA) ELISA, RIA. Unit ­ III Plant viruses: Recent advances in classification of plant viruses Life sciences and other details of TMV and mosaic virus, potato virus X General idea about cyanophages, actinophages and mycoviruses. Unit ­ IV Bacterophages: Classification, Morphology and ultrastructure One step growth curve ( Latent period, eclipse period and burst size) Life cycle: Lytic and Lysogenic cycles of bacteriophages Unit ­ V Animal viruses; classification and nomenclature Life cycles and other details of DNA viruses: herpes, adeno and SV40 Life cycle and other details of RNA viruses: Retroviruses, oncogenic viruses and lentiviruses (HIV), picorna, ortho myxo and paramyxo. MyCOLOGY 1. Classification of Fungi 2. Growth and isolation 3. Mycoses (all types) 4. Laboratory diagnosis of mycotic diseases. 5. Immunity in fungal diseases and value of immuno diagnosis. 6. Role of mycotoxin 7. Antifungal agents 8. Epidemiology of fungal diseases.

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SEMESTER II

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

MSCMLT-303: Parasitology

Unit I (i) (ii) (iii) Unit II (i) (ii) (iii) General principle of host parasite interaction and definitions of terms in this connection. Classification of the parasitic protozoans. Introduction to parasite host and Vectors. Morphology, life cycle and lab diagnosis of E-coli, Trichomonas and Leishmania. Classification of parasitic helminthes. Morphology, life cycle and lab diagnosis of malarial parasite. Morphology, life cycle and lab diagnosis of Ascaries and Taenia.

SEMESTER II

MSCMLT-304: Diagnostic Microbiology

1 Classification of bacteria On bacilli of differential staining Gram,s Stain .( its modification ) ZN .Stain ( its modification ) B ) On basis of their structure Pre ­remit of sample collections-general & disease specific their processing & storage Identification of bacteria on basis of cultural characteristics ,morphological , & serological features. A) Staphylococcus & streptococcus including pneumonococci B) Family Enterobacterical C) Haemophilus bordetlla D) Corynebacterium E) Nessieria .Treponema F) Leptospira ,mycoplasma,chlamydia & Trieagents

2 3

4 Identification of pathogenic & nonpathogenic fungi( Morphologically,biochemically a) Yeast b) Dermatophytes c) Cryptocococci d) Histoplasma e) Nocardia f) Common lab fungal contaminants 5 6 Characteristic diagnostic serological tests in diseases : Cholera,Typhoid,Tuberclosis ,VDRL, ,TPHA, Satellitism.ELISA, PCR. Uerology a)General morphology & ultra structure of virus and growth cycles. b)Viral genome : Their types & symmetry. c) Cultivation of virus in embryonated eggs, primary culture & secondary culture d) Assay methods: Physical & chemical e) Classification 1 On basic of structure 2 On basic of nuclear material f) Clinical diagnosis serological techniques for identification of bacteria : TMV Bacteriophages.HIV . SV 40 ,myxo & paramyxovirus

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SEMESTER II

MSCMLT-305: Practical

Bacteriology Virology & Mycology Parasitology Diagnostic Microbiology Internal Assessment : 20 Marks : 15 Marks : 20 Marks : 20 Marks : 25 Marks

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SEMESTER IV

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

MSCMLT-401: Immunology

Unit ­ I Immune response: Immunity, Type (Innate & adaptive immune response) Organs of Immune System: Primary and Secondary lymphoid organ Ontogeny and phylogeny of Lymphocytes: T and B Lymphocyts, Null Unit ­ II Cell of Immune System: Mononuclear cell and granulocytes, Antigen presenting cell. Antigen, Heptanes: Factors effecting immunogenicity,m epitopes (Properties of it) Antibodies: Structure , Types and function Unit ­ III Complement System : Role of complement system in immune response, complements and Components and activation pathways. Monoclonal antibodies: Production characterization and applications in diagnosis, therapy and basic research. Antigen-Antibody interaction, avidity & affinity measurement. Unit ­ IV Hypersensitivity: Definition, factor causing hypersensitivity Common hypersensitivity reaction, types, classification based on the time taken for reaction Auto Immune disease Unit ­ V Immunodiagnostics: Precipitation techniques, Agglutination, Fluoresence techniques ELISA, RIA Double diffusion and Immuno-electrophoresis. Immunidiagnostics: VDRL test, Widal test, RA factor, Blood grouping, Rh typing, Comb's test

SEMESTER IV

MSCMLT-402: Cytology

Cytological Staining Cytological preparation with special emphasis on MGG, Pap stains Cytological Fixatives Cytological Screening Quality Control in Cytology Lab Collection of Various cytological specimens Vagina (All types of smear) Fluids (CSF, Ascitic, Pleural) Urine (Millipore method) Cervical Cytology Basis of detection of malignant & premalignant testing Hormonal assessment with cytological techniques, sex chromatins & Pregnancy test Aspiration cytology principles, indications & utility of the techniques with special emphasis on role of cyto-technician in FNAC System

SEMESTER IV

MSCMLT-403: Histopathology

Unit ­ I Theory of Histopathology, Reception of specimens, Histopathology of Tumor cell Histopathology of Liver, Kidney, Adrenal, Ovary, Testies Method of preparing stains & Fixatives. Unit ­ II Theory of Tissue processing and embedding Theory of H & E staining Use of Microtome, Tissue section cutting Unit ­ III Embedding and preparation of blocks Fixation of Tissue with DPX mount Theory of frozen section preparation Unit ­ IV Preparation of smear for Fine needle aspiration cytology

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Pap's smear theory and identification of cells in a normal vaginal smear Stool examination: normal, abnormal constituent. Unit ­ V Normal and abnormal constituent of Urine Normal and abnormal constituent of aminotic fluid Normal and abnormal constituent of Semem analysis

SEMESTER IV

MSCMLT-404: Research Methodology & Techniques

Introduction to Research: Definition, Scope, Limitations, and Types. Objectives of Research Research Process Research Designs Data Collection: Secondary Data, Primary Data, and Methods of Collection. Scaling Techniques: Concept, Types, Rating scales & Ranking Scales Scale Construction Techniques, Multi Dimensional Scaling. Sampling Designs: Concepts, Types and Techniques Sample Size Decision Theory of Estimation and Testing of Hypothesis Small & Large Sample Tests, Tests of Significance based on t, F , Z test and Chi-Square Test. Designing Questionnaire. Interviewing. Tabulation, Coding, Editing. Interpretation and Report Writing.

SEMESTER IV

MSCMLT-405: Practical

Immunology Cytology Histopathology Research Methodology & Techniques Internal Assessment : 20 Marks : 15 Marks : 20 Marks : 20 Marks : 25 Marks

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M.Sc. (Clinical Microbiology) (MSCCMB)

SEMESTER I

MSCCMB-101: Human Anatomy

ANATOMY Introduction of anatomy and Histology, Elementary Histology of cell, Tissues of the body organs and system, Elementary Anatomy and Histology of :a.Skeletel System Development of bones, types of bones, Micro-anatomical and gross structure of bones, Osteology of human skeleton and various movement of joints. b. Muscular System Structure and type of muscles in human body, important muscles and their group action. c.Criculation System Structure of heart and blood vessels, Systemic criculation, pulmonary circulation, Portal criculation, and coronary circulation. d.Lymphatic System Lymph vessels, Lymph nodes and Lymphoid organs, their structure and functions. e.Digestive System Gastrointestinal tract and associated glands (Salivery Glands, Liver, Pancreas etc). f .Respiratory System Trachea, Lungs including other air passages. g.Urinary System Kidney, ureter and urinary bladder etc. h.Endocrine System Thyroid glands, Parathyroid glands, Adrenal glands and Pituitary glands. i.Femal and Male reproductory organs System. j. Skin and its appendages, k.Special sense organs: Eye, Ear, Nose Taste buds, Subcutaneous sense organs. l.Nervous System: Brain, Spinal cord and peripheral nerves.

SEMESTER I

MSCCMB-102: Human Physiology

1.Blood: Blood volume, composition and function of blood, haemopoesis, blood coagulation, blood groups, body fluids. a.Cardiovascular System : General plan of cirulatory system, function of heart and blood vessels (arteries, arterioles, capillaries and veins) heart sound and E.C.G. nervous control of heart and blood vessels, regular of blood pressure. b.Respiratory System Functional anatomy of respiratory system, mechanism of breathing and exchange of gases in the lungs. Regulation of respiration, Oxygen and. carbondioxide carriage, anoxia, dysproes, cyanisis, artificial respiration and pulmonary function test. c.Gastrointestinal System : Alimentary canal and its various glands, digestion of food in mouth, stomach and small intestines, gastro-intestinal tract

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movements and absorption. Function of liver and liver function tests and metabolism. d.Excretory System Structure and function of kidney and Urinary bladder, Structure and function of skin. e.Endocrine Glands Reproductive System: Endocrine glands and their function. Regulation of endocrine secreation. Physiology of male and female reproductive System. f.Muscular System: Types of muscles, innervation of muscles, neuromuscular transmission, mechanism of muscular contraction. Nervous System : Neurone and its function, spinal cord and reflex action, sensory end organs and sensory path ways, cerebral cortex and motor path ways. Maintenance of posture and locomotion, automatic nervous system, Physiology of vision, hearing test and olfaction.

SEMESTER I

MSCCMB-103: Clinical Biochemistry

Laboratory management and planning,reception and recording of specimens,Cataloging and indexing, Maintenance of laboratory records.

Knowledge of calibration and volumetric glass wares the underlying principles,care and use of analytical balance,electrical balance, Photoelectric colorimeters,flame Phottometer,PH meter,Absorptionmeter,Visual Colorimeters,Spectrometers,and Electrophoretic apparatus. Stiring and cleaning of glass wares knowledge of common types of pipettes,Flasks ,Funnels, other glass waes and Kipp's apparatus etc. Anticoagulants,collection and preservation of specimens. Basic knowledge of Physical Chemistry: Atom ,molecule ,valancy,ion,atomic weight,molecular weight,acid,base salt-acidimetrty and alkalimetry,reduction,and ionization.Basic knowledge of element,metals and non-metals,their compund and salts,organic solvents,cobohydrates,fats,protein,aminoacids,urea,uric acid and enzyme,indicators. Symbols of molecular weight of somecommonly used compounds-Acetric acid,Amonium Hydroxide,clcium chloride copper sulphATE,hYDROECHLORC acid Nitric acid sodium carbonate sodium chloride, Sodium Hydroxide,Sulphuric acid,carbon,Cholride,Hydrogen,Oxygen,Nitogen,Phosphorus,Pottasium Silver,Sodium and Sulpher. Molar Normal and Abnormal solutions ,PH buffer solutions acid Preseration of common solutions,Gravimetric and Volumetric methods,Electrophoresis. Chemical Examination of Urine for Protin,Sugar,Blood,Ketone bodies,Bile Pigment and salt,Urobillinogen,Calcium Chloride etc. Preparation of solutions,Principle ,Specimen,Procedure Calculation ,normal value of each tests.Preparation of Protin free Filtrate, Liver function test: a.Van den barg raction b.Serum Bilirubin-(Malloy and Evelyn method of jendrassik and Grof method) c.Serum Protin-(Biuret method) d.Serum albumine.Serum Globulinf.Icteric indexLipids: a.Serium Cholesterol Glucose Metabolism: a.Blood sugar fasting and post prendal-(Toluidine methods folin ­wu and Glucose oxidase methods) b.Glucose Tolerance test c.Urine Glucose.

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SEMESTER I

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

MSCCMB-104: Histopathology

Introduction to Histology, the cell, cell Organelles, nucleus, cell division, tissues, fresh & fixed tissues. Different types of Embedding Viz. Wax, Resin, Cryostat etc. Basic Cytology Fixation of tissue, different kind of fixatives, sample fixative, compound fixative, formaldehyde, mercusic chloride, osmium, Picric acid, alcohols, other acids, formaline, buffered fomaline, osmic acid, zenleer soln, he;ly's soln, cytological fixatives, nuclear fixatives, fixation of smear etc., decalcification, method of decalcification, assessment of decalcification, soln for decalcification. Processing of tissue, dehydration, impregnation in the wax, manual and automatic tissue processor, gelatin embedding, celloidin embedding, double embedding, cytological fixatives, preparation of different smears, vaginal, sputum, membrane. Microtome, instrument, principle, use in section cutting, parts and working of commonly used microtome, different kinds of microtome, rotary, base sledge, sliding, low temperature microtome, cryostat, microtome knives, homing and stropping knives. Section cutting of paraffin sections, section preparation from frozen sections, fixing of tissue to slide, preparation of celloidin section and fixation. Staining techniques, natural dyes, synthetic dyes, basic and acidic dyes, haematoxylin staining, Pap, flucker & Conn, methanamine silver nitrate, ziehl neelsen's stain, propylene glycol sudan technique, papanicolaou, harn's alum, Haematoxylin, acridine orange technique.

SEMESTER I

MSCCMB-105: Introductory Biology

Unit I Living World Biology & Its Branches; relationships with other sciences; scientific methods in Biology; historical breakthroughs; scope of biology and career options; role of Biology in dispelling myths and misbelieves; characters of living organisms, (elementary idea of metabolism, transfer of energy at molecular level, open and closed systems, omoeostasis, growth and reproduction, adaptation, survival, death). Origin and evolution of life - theories of evolution; evidence of evolution; sources of variations ( mutation, recombination, genetic drift, migration, natural selection); concept of species; specification and isolation (geographical and reproductive); origin of species. Unit II Diversity of Life Variety of living organisms, Systematic; need, history and types of classification (artificial, natural, polygenetic); biosystematics; binomial nomenclature; Two kingdom system, Five kingdom System, their merits and demerits, status of bacteria and virus; botanical gardens and herbia; zoological parks and museums. Unit III Cell and Cell Division Cell as a basic unit of life - discovery of cell, cell theory, cell as a self - contained unit; procaryotic and eukaryotic cell; unicellular and multicellular organisms; tools and techniques ( compound microscope, electron microscope and cell fractionation); Ultrastructure of prokaryoytic and eukaryotic cell - cell wall, cell membrane - unit membrane concept (flauid mosaic model); membrane transport; cellular movement (exocytosis, endocytosis); cell organelles and their functions - nucleus, mitochondria, plastids, endoplamasic reticulum, Gogli complex, lysosomes, lysosomes, microtubules, centriole, vacuole, cytoskeleton, cilia and flagella, ribosomes.

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Molecules of cell; inorganic and organic materials - water, salt, mineral ions, carbohydrates, lipids, amino acids, proteins, nucleotides, nycleic acids (DNA and RNA); Enzymes (Properties, chemical nature and mechanism of action); vitamins, hormones and steroids. Unit IV Genetics Continuity of life - heredity, variation; mendel's laws of inheritance, chromosomal basis of inheritance; other patterns of inheritance - incomplete dominance, multiple allelism, quantitative inheritance. Chromosomes - bacterial cell and eukaryotic cell; parallelism between genes and chromosomes; genome, linkage and crossing over; gene mapping; recombination; sex chromosomes; sex determination; sex linked inheritance; mutation and chromosomal aberrations; Human genetics - methods of study, genetic disorders. DNA as a genetic material - its structure and repliaction; structure of RNA and its role in protein synthesis; Gene expression - transcription and translation in prokaryotes and eukaryotes; regulation of gene expression, induction and repression - housekeeping genes; nuclear basis of differentiation and development; oncoenes. Basics of Recombinant DNA technolgy; cloning; gene bank; DNA fingerprinting; genomics principles and applications, transgenic plants, animals and microbes. Unit V Morphology of Plants and Animals Morphology - root, stem and leaf, their sturucture and modification; Inflorescence, flower, fruit, seed and their types;Description of Poaceae, Liliaceae, Fabaceae, Solanaceae, Brassicaceae and Asteraceae. Morphology of animals - salient features of earthworm, cockroach and rat; tissue systems, structure and function of tissues - epithelial, connective, muscular and nervious. Practical 1. Study of parts of Compound Microsope 2. Study of mitosis in onion root tip and animal cell (grasshopper) 3. Study of meiosis in onion flower buds, and testis iof grasshopper. 4. Study of cyclosis in leaf cell of Hydrilla, or Tradescantia and in Paramoecium. 5. Study of cell wall components ( cellulose, lignin, suberin and mucilage). 6. Study of mitochondria by staining with a Janus Green.

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

7. Study of specimens and their identification with reason - Bacteria, Oscillator, Spirogyra, Rhizopus, mushroom/bracket fungi, yeast, liverwort, moss, fern, Pinus, one monocotyledon, one dicotyledon and lichens. 8. Study of characters of specimens and identification with reason - Amoeba, Hydra, Liver - Fluke, Ascaris, Leech, Earthworm, Prawn, Silk moth honey bee, snail, Starfish, Dogfish, Rohu, Frog, Lizards, Pigeon/ any other bird and rabbit/ any other mammal. 9. Study of squamous epithelium, muscle fibres, nerve cells and mammalian blood film through temporary/permanent slides. 10. Study of external morphology of earthworm, cockroach, from and rat through models.

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SEMESTER I UNIT I

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

MSCCMB-106: Communication & Soft Skills

Essentials of Grammar: · · · · UNIT II Office Management: · · · · · · · UNIT III Letter & Resume Writing: · · · · · · · UNIT IV Presentation Skills: · · · · · · · · · · · UNIT V Interview Preparation: · · · · · · · · Types of Interview Preparing for the Interviews Attending the Interview Interview Process Employers Expectations General Etiquette Dressing Sense Postures & Gestures

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Parts of Speech Punctuation Vocabulary Building Phonetics

Types of Correspondence Receipt and Dispatch of Mail Filing Systems Classification of Mail. Role & Function of Correspondence MIS Managing Computer

Types of Letters-Formal / Informal Importance and Function Drafting the Applications Elements of Structure Preparing the Resume Do's & Don'ts of Resume Helpful Hints

Importance of Presentation Skills Capturing Data Voice & Picture Integration Guidelines to make Presentation Interesting Body Language Voice Modulation Audience Awareness Presentation Plan Visual Aids Forms of Layout Styles of Presentation.

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

UNIT VI Group Discussion & Presentation: · · · · · Definition Process Guidelines Helpful Expressions Evaluation

(Note: Every student shall be given 15 minutes. of presentation time & 45 minutes of discussion on his/ her presentation.) The student will be evaluated on the basis of : his / her presentation style Feedback of Faculty & Students General Etiquette Proficiency in Letter Drafting / Interview Preparation The paper is internal and at least 3 tests will be taken. Best 2 of 3 shall account for final grades (70% Test & 30% Presentation)

SEMESTER I

MSCCMB-107: Practical

Human Anatomy Human Physiology Clinical Biochemistry Histopathology Introductory Biology Internal Assessment : 15 Marks : 15 Marks : 15 Marks : 15 Marks : 15 Marks : 25 Marks

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SEMESTER II

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

MSCCMB-201: General Bacteriology

1.Cell Structure Morphology, Stainings, detailed structure in comparison to Ecomytic Cell, Bacterial anatomy. 2.Microscopy Various optical methods available for viewing microorganisms and their application, 3.Overview of microbial world Classification Purpose Basic Principles and classification systems. 4.Growth, survival of microorganisms -growth curve -growth parameters -Survival of micro organisms in natural environment. -Role of antimicrobial agents 5.Cultivation of Micro organisms -growth requirements -sources of metabolic energy -Bacterial nutrition -methods of cultivation 6.Microbial Metabolism -Metabolism of bio synthesis and growth. -bio synthesis pathway -energy yielding metabolism -regulation of metabolic pathways. 7.Bacterial Genetics -mutation -conjugation -genetic mechanism of drug resistance -genetic engineering 8.Destruction of micro organism. -sterilization and disinfection -antimicrobial agents 9.General principles in Diagnostic Microbiology -collection and handling of various samples -laboratory safety -antimicrobial susceptibility and assays -lab animal an introduction

SEMESTER II

MSCCMB-202: Immunology

Unit ­ I Immune response: Immunity, Type (Innate & adaptive immune response) Organs of Immune System: Primary and Secondary lymphoid organ Ontogeny and phylogeny of Lymphocytes: T and B Lymphocyts, Null Unit ­ II Cell of Immune System: Mononuclear cell and granulocytes, Antigen presenting cell. Antigen, Heptanes: Factors effecting immunogenicity,m epitopes (Properties of it) Antibodies: Structure , Types and function Unit ­ III Complement System : Role of complement system in immune response, complements and Components and activation pathways. Monoclonal antibodies: Production characterization and applications in diagnosis, therapy and basic research. Antigen-Antibody interaction, avidity & affinity measurement. Unit ­ IV Hypersensitivity: Definition, factor causing hypersensitivity Common hypersensitivity reaction, types, classification based on the time taken for reaction Auto Immune disease Unit ­ V Immunodiagnostics: Precipitation techniques, Agglutination, Fluoresence techniques ELISA, RIA Double diffusion and Immuno-electrophoresis. Immunidiagnostics: VDRL test, Widal test, RA factor, Blood grouping, Rh typing, Comb's test

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SEMESTER II

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

MSCCMB-203: Haematology

1. Introduction of haematology, composition of blood, cellular and humoral components. 2. Reception labeling and recording of laboratory investigations. 3. Quality control in Individual Laboratory. 4. Cleaning of glass wares, pipettes, ESR tubes and couting chamber. Care of syringes and Needles. 5. Error in procedure / Sources of Error . 6. Preparation of capillary pipettes, Distilled water, reagents Buffer and Normal solutions. 7. Collection of specimen, difference between capillary and artery and venous blood specimen use of each. 8.Preparation and examination of thin, thick and wet blood films for blood parasite. Stained blood films -Leishman's, wright's, Simon's stain and supravital. 9.Preparation of solutions. 10.Normal and abnormal blood cells morphology -erythrocytes, leucocytes, platelets, reticulocytes count. 11.Recognition of principle blood pictures, knowledge about the Anaemia and Leukamias. Abnormalities of RBCS -shape, size, colour, premature RBCS (RBCS series and WBCS series). 12.Methods of measuring haemoglobin including spectro photometric, method PCV, MCH, MCHC & MCV and ESR. 13.a) Total WBC and RBC count -Micropipette and bulk dilution methods. b) Packed cell volume (Haematocrit) -macro and or micro methods. c) Calculation and interpretation of cell Indices (absolute values) d) Differential count of WBC, including Arneth,and Schilling counts. e) Absolute Eosinophil counts. f) Platelets count direct and indirect. g) Bleeding and clotting time -Duke and Ivy methods. h) Erythrocyt~s sedimentation rate. i) Normal and Abnormal values. 14.a) Recognition of blood parasities. b) Malaria and its life cycles and Differential diagnosies of malaria parasite. c) Kala -azar (L.D. bodies) d) Spirochaetes. e) Trypanasomes f) Microfilaria

SEMESTER II

MSCCMB-204: Blood Banking

1. Principal of Immuno haematology. 2. Blood Bank management and planning, The reception and recording of specimens, Cataloging and indexing, Maintenance of Blood Bank records. 3. Knowledge of Maintenance and working of blood strong cabinets. 4. Preparation and sterilization of transfusion sets. 5. Preparation and use of ACD (acid citrate dextrose), EDTA, Heparin, CPD Citrate Phosphate Dextrose) . 6. Theory including inheritance and nomenclature of ABO and Rh, blood group system other blood groups. 7. Subgroups of ABO blood group system. 8. Techniques for determination of various blood groups. 9. Sources of errors in grouping and their elemination. 10. Selection and preparation of grouping sera. 11. Group sera titration. 12. Criteria for selecting and rejecting Donors. 13. Method & Precaution of Blood Collection. 14. preservation of Blood. 15. Ante -natal investigation. 16. Coombs test compatibility and techniques -Direct and Indirect methods. 17. Cross matching techniques. 18. Investigation of Malaria and Microfilaria. HIV test ABS Ag. 19. Investigation of VDRL I Kahn test. 20. Blood collection and preservation. 21. Quality control in Blood Bank. 22. Disposal of used and other materials of Blood Bank.

.

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SEMESTER II

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

MSCCMB-205: Practical

General Bacteriology Immunology Haematology Blood Banking Internal Assessment : 20 Marks : 20 Marks : 15 Marks : 20 Marks : 25 Marks

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SEMESTER III

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

MSCCMB-301: Systematic Bacteriology

Systematic Study of microbes of medical Importance I.a) Gram +ve cocci b) Gram -ve cocci c) Gram +ve Bacilli d) Gram -ve Bacilli e) Vibriocholera f) Brucella g) Haemophilus h) Bordetella i) Spirochaetes j) Anaerobic Bacteria, etc. 1. Study of the morphology I cultural, biochemical and other biological properties and characteristics of above said medically important Bacteria. 2. The mechanism of virulence and pathogenesis and pathology. 3. The disease caused by them epidemiology treatment prevention and control. SEMESTER III

MSCCMB-302: Applied Microbiology

Normal flora of the human body Collection of clinical specimens for diagnosis and method of processing antibiotic susceptibility testing and its interpretation and reporting. Nosocomial infection -surveillance and control. Diseases transmitted through air, water, milk and food. Quality control in diagnostic microbiology. National programmes for control of infectious diseases. Etiology, laboratory diagnosis of infectious diseases of each system.

SEMESTER III

MSCCMB-303: Basic Cellular Pathology

Study of body tissues- Epithelial tissue, connective tissue including bone and cartilage, muscular tissue Study of various systems- Circulatory system, alimentary system, digestive system including liver, pancreas and gall bladder, Respiratory system Microscopy - Working principle, maintenance and applications of various types of microscopes -Darkground microscope, polarizing microscope, phase contrast microscope, Interference microscope, U.V. light microscope, micrometry Metachromasis and Metachromatic dyes Haematoxylin stain, its importance in histology. Stains, cytological preparation with special emphasis on MGG, PAPANTICOLOU stains. Special stains like PAS, Mucicarmine, Alcian blue, Schmorl, Acid phosphatase

SEMESTER III

MSCCMB-304: Molecular Biology

Basic knowledge as applicable to molecular diagnostics and molecular epidemiology. Basic knowledge only of the following 1. Recombinant DNA technology. 2. Southern, northern and western blotting. 3. DNA amplification techniques. 4. Diagnostic PCR, different methods of PCR product detection (liquid hybdridization, Elisa.) 5. Genotyping of microbes and viruses.

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SEMESTER III

MSCCMB-305: Practical

Systematic Bacteriology Applied Microbiology Basic Cellular Pathology Molecular Biology Internal Assessment : 15 Marks : 20 Marks : 20 Marks : 20 Marks :25 Marks

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SEMESTER IV

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MSCCMB-401: Virology

Unit ­ I General morphology and ultra structure of Viruses: Capsids- Helical Symmetry, icosahedral symmetry and complex symmetry. Envelope: Glycoprotein and matrix protein Viral genome: their types and structure Unit ­ II Cultivation of Viruses in embryonated eggs, experimental animals and cell culture: primary and secondary cell culture, suspension cell culture and monolayer cell cultures. Assays of viruses: physical and chemical methods of assays (protein nuclei acid, radioactivity traces, electrons microscopy, plaque method, pock counting method, end point method and infectivity of plant viruses). Serological methods: haemagglutination haemagglutinationinhibitation, complement fixation, immunofluroscence assays (IFA) ELISA, RIA. Unit ­ III Plant viruses: Recent advances in classification of plant viruses Life sciences and other details of TMV and mosaic virus, potato virus X General idea about cyanophages, actinophages and mycoviruses. Unit ­ IV Bacterophages: Classification, Morphology and ultrastructure One step growth curve ( Latent period, eclipse period and burst size) Life cycle: Lytic and Lysogenic cycles of bacteriophages Unit ­ V Animal viruses; classification and nomenclature Life cycles and other details of DNA viruses: herpes, adeno and SV40 Life cycle and other details of RNA viruses: Retroviruses, oncogenic viruses and lentiviruses (HIV), picorna, ortho myxo and paramyxo.

SEMESTER IV

MSCCMB-402: Mycology

MyCOLOGY 1. Classification of Fungi 2. Growth and isolation 3. Mycoses (all types) 4. Laboratory diagnosis of mycotic diseases. 5. Immunity in fungal diseases and value of immuno diagnosis. 6. Role of mycotoxin 7. Antifungal agents 8. Epidemiology of fungal diseases.

SEMESTER IV

MSCCMB-403: Parasitology

PARASITOLOGY a. General Principles of host parasite interactions and definitions of terms in this connection. b. Morphology, life cycle and pathogenesis of the parasites listed below. The students should know the medical importance, laboratory diagnostic methods, drugs used for therapy and Epidemiology. PROTOZOA 1. Intestinal amoebae. 2. Free living pathogenic amoebae 3. Intestinal and genital flagellates 4. Haemoflagellates 5. Ciliates of medical importance 6. Malarial parasite HELMINTHS 1. Nematodes (a) Intestinal (b) Tissue 2. Trematodes infection in man. 3. Cestodes infecting man 4. Larval infections in man.

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SEMESTER IV

MSCCMB-404: Research Methodology & Techniques

Introduction to Research: Definition, Scope, Limitations, and Types. Objectives of Research Research Process Research Designs Data Collection: Secondary Data, Primary Data, and Methods of Collection. Scaling Techniques: Concept, Types, Rating scales & Ranking Scales Scale Construction Techniques, Multi Dimensional Scaling. Sampling Designs: Concepts, Types and Techniques Sample Size Decision Theory of Estimation and Testing of Hypothesis Small & Large Sample Tests, Tests of Significance based on t, F , Z test and Chi-Square Test. Designing Questionnaire. Interviewing. Tabulation, Coding, Editing. Interpretation and Report Writing.

SEMESTER IV

MSCCMB-405: Practical

Virology Mycology Parasitology Research Methodology & Techniques Internal Assessment: 25 Marks : : : : 20 Marks 15 Marks 20 Marks 20 Marks

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M.Sc. (Biotechnology) (MSCBT)

SEMESTER I

MSCBT-101: Introductory Biology

1. Nature and Scope of Biology: Basis and origin of life, Living and non ­ living features of life process. Energy Transformation. common

2. Kingdoms of life, or Biological Classification, species and population, Biotic community, Biosphere. 3. Cell as a unit of life : Prokaryotic and Eukaryotics cell, Plant and animal cell, cell structure, membrane organization and cell organelles. Cell function, cell division mitosis and meiosis, cell cycle . 4. Introduction to Bio Molecules: Structure and properties of mono, oliga and poly Saccharides Structure and properties of fatty acids, glycerolipids, phospholipids, glycolipids, steroids. Structures and Properties of amino acids peptides and protein. Structure and properties of purines, Pyrimidines, nucleosides, nucleotides, polynucleotides, Ribonucleic acids and Deoxyribonucleic acids, nucleoprotein complexes. 5. Metabolism: Introduction of Respiration and Photosynthesis.

SEMESTER I

MSCBT-102: Molecular Biology ­ I

Unit I:-Cell:- Cell organelles: Structure function, structure of mitochondria and organization of respiratory chain, organization of cytoskeleton and nucleic. Unit -II:- STRUCTURE & PROPERTIES OF DNA Discovery of DNA as the genetic material, Structure of DNA (A,B&Z forms ), concept & definition of the genome, C-value paradox, denaturation & renaturation of DNA, repetitive & non-repetitive DNA, reassociation kinetics. Cot curve, Rot curve, chemical & kinetic complexitv of DNA, supercoiling in DNA. Unit -III :- PROKARYOTIC AND EUKARYOTIC GENOME ORGANIZATION:-Organization of viral genome, organization of bacterial genome, chromosome structure in eukaryotes, nucleosome model, chromatin structure, satellite DNA, polytene chromosomes, lampbrush chromosomes, B chromosomes-Evolution of the gene concept, definition of gene, interrupted genes, multigene families & pseudogenes, overlapping genes, nested genes, open reading frames. Unit-IV:-DNA REPLICATION:- General features of DNA replication in prokaryotes & eukaryotes, enzymology of DNA replication, regulation of DNA replication Unit-V:- GENE MUTATION, REPAIR & RECOMBINATION:-Definition & types of mutation, mutagenic agents & their mode of action, reversion & suppression of mutation. DNA repair mechanisms : light dependent repair, excision repair, mis-match repair Post-replication repair, sos response. DNA recombination mechanisms : holiiday model, Rec A, Rec BCD, gene conversion

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SEMESTER I

MSCBT-103: Bioenergetics & Metabolism

Unit I Introduction to Biochemistry, define Carbohydrate, nomenclature, classification, structure, chemistry and properties. Biological functions of carbohydrate. Carbohydrate metabolism : Basic concept, glycolysis, kreb cycle, pentose phosphate pathway and regulation. Polysaccarides : Bacterial cell wall, peptidoglycans and glycolipid. Unit II Lipid : Define Lipid, nomenclature, classification structure, chemistry and properties. Lipids with specific biological function. Lipoproteins and biological membrane, micelles and liposomes. Lipid metabolism : Biosynthesis and degradation of fatty acid. Prostaglandins, leukotrienes and thromboxanes. Unit III Amino acid : Structure, classification, properties and function. Methods for isolation and characterisation of protein. Chemical Synthesis of peptides; study of specific protein like haemoglobin, myoglobin and plasma protein. Protein : Classification and properties. Fluorimetric method for determination of N-terminal amino acid. Unit IV Nucleic acid : Structure, chemistry and properties of purines, pyrimidines, nucleosides & nucleotides, Polynucleotide. Biosynthesis and degradation of purines and Pyrimidines. Enzyme : Basic concept. Vitamins and their deficiency. Unit V First and second laws of thermodynamics & concept of free energy. High energy phospho Compounds, ATP cycle, structural basis of free energy during hydrolysis of ATP. Hormones : Hormones receptors and intracellular messengers, Adenylate cyclase, Protein kinase and phosphodiesterase. Porphyrins : Classification and Structure.

SEMESTER I

MSCBT-104: Microbiology

U n i t - 1:- THE WORLD OF BACTERIA:General characteristics & classification of bacteria according to Bergey's manual of systematic bacteriology. Nutritional requirements & physical conditions necessary for cultivation of bacteria, properties of bacteriological media. Unit II:HISTORY AND SCOPE OF MICROBIOLOGY:- Milestone in

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Microbiology- Recent

development and future trends. Anatomy of Prokaryotes & Protozoa and Viruses.

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Eukaryotes-Morphology-ultrastructure

of Bacteria, Fungi, Algae,

Unit - III :- MICR0BIAL METABOLISM & STAINING TECHNIQUES Classification of bacteria on the basis of their nutritional requirements, transport of substances across membranes. Procedure and principle involved in gram's staining, acid fast staining, flagella staining, endospore staining.

Unit IV:- Microbial growth & nutrition :- Definition of growth, mathematical expression of growth, measurement of growth field; synchronous growth, continuous culture, effect of environmental factor on growth. Unit V:- Microbial Ecology:- Rhizosphere, phylloplane & role of microorganism is productivity of ecosystem. Interaction between microorganism, and with plants and animals. Biogeochemical cycle. Microbes & bio-deterioration.

SEMESTER I

MSCBT-105: Biophysical Chemistry & Instrumentation

Unit I Electronic theory of valency dipole moment. Electron displacement, electronic effect, resonance. The hydrogen bond, hydrophobic interactions. Atomic theory and molecular orbital. Hybridization. Free radicals in biological systems, antioxidants. Isomerism : Structural and steroisomerism optical activity, meso compounds. Specific rotation, chirality, chiral, enantiomers, conformation and configuration. Conformational analysis of monosacharides, boat and chair forms, eclipsed. Mutarotion, glycosides epimers. Unit II Centrifugation : Principle, types, analytical and prepared centrifugation, differential density, gradient centrifugation, sedimentation and coefficient centrifuge and its application. Electrophoresis : Principles, types and application (paper, starch, gel polyacrylamide and agar electrophoresis. Chromatography : Principle type and application, (Paper, thin layer, gas ion exchange and molecular sieve). Affinity chromatography, HPLC, FPLC. Unit III Photometry : Basic Principle of UV-Vis spectrophotometry and colorimetry. Fluorimetry : Principle, instrument and application. Flame techniques, atomic absorbtion spectroscopy. Isoelectric focusing and Isotachophoresis. Unit IV Microscopy : Light, phase contrast, interference and electron microscopy. X-ray crystallography. Radioactivity : Radioisotopes, half life, units. Geiger Mueller counter. Scintillation ; Liquid, Crystal and gamma counter. Unit V

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ESR : Principle, instrumentation and its application. NMR : Principle, instrumentation and its application. ORD and CD : Principle, instrumentation and its application. Polarimetry : Principle, instrumentation and its application. INSTRUMENTATION PRACTICAL Practical course of Semester I Verification of Beer's law. Determination of absorbtion maxima. Electrophoresis of Proteins - native and under denaturing conditions. Amino acid and carbohydrate separations by paper and thin layer chromatography. Separation of blood cells by density gradient centrifugation. Chromatographic method for seperation of macromolecules. Separation of subcellular organelles by differential centrifugation. Electrophoresis of DNA - linear, circular and super coiled. SEMESTER I

MSCBT-106: Communication & Soft Skills

UNIT I Essentials of Grammar: · · · · Parts of Speech Punctuation Vocabulary Building Phonetics

UNIT II Office Management: · · · · · · · UNIT III Letter & Resume Writing: · · · · · · · UNIT IV

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Types of Correspondence Receipt and Dispatch of Mail Filing Systems Classification of Mail. Role & Function of Correspondence MIS Managing Computer

Types of Letters-Formal / Informal Importance and Function Drafting the Applications Elements of Structure Preparing the Resume Do's & Don'ts of Resume Helpful Hints

Presentation Skills: · · · · · · · · · · · UNIT V Interview Preparation: · · · · · · · · UNIT VI Group Discussion & Presentation: · · · · · Definition Process Guidelines Helpful Expressions Evaluation Types of Interview Preparing for the Interviews Attending the Interview Interview Process Employers Expectations General Etiquette Dressing Sense Postures & Gestures

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

Importance of Presentation Skills Capturing Data Voice & Picture Integration Guidelines to make Presentation Interesting Body Language Voice Modulation Audience Awareness Presentation Plan Visual Aids Forms of Layout Styles of Presentation.

(Note: Every student shall be given 15 minutes. of presentation time & 45 minutes of discussion on his/ her presentation.) The student will be evaluated on the basis of : his / her presentation style Feedback of Faculty & Students General Etiquette Proficiency in Letter Drafting / Interview Preparation The paper is internal and at least 3 tests will be taken. Best 2 of 3 shall account for final grades (70% Test & 30% Presentation) SEMESTER I

MSCBT-107: Practical

Introductory Biology Molecular Biology ­ I Bioenergetics & Metabolism Microbiology Biophysical Chemistry & Instrumentation Internal Assessment : 15 Marks : 15 Marks : 15 Marks : 15 Marks : 15 Marks : 25 Marks

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SEMESTER II

MSCBT-201: ENZYMOLOGY & ENZYME TECHNOLOGY

Unit I (i) Enzyme: Historical aspects, nomenclature and classification. (ii) General properties of enzymes and the factors that affect their activity and the associated changes. (iii)Extraction assay and purification of enzymes. (iv)Sub-cellular localization and organization of enzymes Unit II (i) Enzyme kinetics (steady state), determination of Km value and studying, kinetics using, Lineweaver-Burke plot, Eadie Hofstee plot "and: IHans-Woolf equations. (ii) Enzyme inhibitors-Presteady state kinetics-fast kinetics to elucidate the intermediate and rate limiting steps (flow and relaxation techniques)Complex kinetics and analysis. (iii) Allosteric enzyme. (iv) Rapid reaction techniques. Unit III (i) Enzyme specifity- Evidences for enzyme substrate complex Nucleophilic and electrophilic attack Role of metal ions in enzyme catalysis. (ii) Mechanism of enzyme action - (lysozyme, chymotrypsin), DNA polymerase, .Rnase etc. zymogens and enzyme actiyators, active site determination. (iii) Regulations of enzymes (iv) Allosteric interactions and product inhibition. Unit IV (i) Coenzyrnes (ii) Isoenzymes & metalloenzymes . (iii) Membrance bound enzymes-their extraction assay. (iv)Lipid-protein interaction and the effect of fluidity on enzyme activity. Unit V (i)Immobilisation ; Principle, kinetics of immobilised systems. (ii)Enzyme immobilisation : Principle, method, activity and application. (iii.) Effect of immobilization on : activity, kinetics parameters and stability (iv)Application of immobilized enzyme.

Practical Q1. Q2. Q3. Q4. Q5. Q6. Q7. Q8. Study of transduction, transformation, conjugation in E.coli. Study of mutation is E.coli. Assay of Enzyme activity. Kinetic studies on enzyme. Alkaline phorphatase estimation. Elisa test Immobilization of Enzymes. Urease estimation by colorimetric method.

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SEMESTER II

MSCBT-202: BINFORMATICS

Overview of Bio-Informatics- Database types, sequence database-nucleotide and protein sequence database, primary and secondary database, Gene Bank, Structure database-protein Data Bank (PDB), Visualization of structural information, Genomics and the genome. Project sequencing and sequence assembling using computers.

SEMESTER II

MSCBT-203: MOLECULAR BIOLOGY-II

Unit - I :- TRANSPOSABLE GENETIC ELEMENTS Discovery & definition of transposons, simple transposons ( IS elements ), composite transposons (Tn3, Tn5, Tn9, TnlO ), Ac/Ds elements in maize, P elements in drosophila, Retxotransposons, mechanisms of transposition.

Unit II:- Gene Expression:- Structure, classes & function of RNA. RNA transcription and processing in Eukaryotes and prokaryotes genetic code and protein synthesis Transcription and translational controls.

Unit III:- Regulation of Gene Expression- The operon concept regulatory gene, promoter gene, operator gene and structural gene, role of CAMP and CRP in gene expression, catabotite repression, induces, repressor corepressor. Brief account of Eukaryotic gene expression (Britson & Davidson model). Protein localization: synthesis of secretary and membrane protein.

Unit IV:-

Gene as unit of Mutation & Recombination :-

Physical and Chemical Basis of Mutation: Mechanism of mutagenesis, Mutation of DNA & protein levels. Recombination is Bacteria - Transformation, transduction and conjugation, mechanism of gene transfer and application.

Unit V:- Oncogenes and their properties ; classification, characteristics and significance in development, Differentiation and Carcinogens . An overview of Apoptosis its phases and significance, Apoptosis in pathogenesis & therapeutic implication. Practical Ql. Q2. Q3. Q4. Isolation of genomic DNA from bacterial cells. Isolation of plasmid DNA from bacterial cells. Isolation of genomic DNA from plant cells. Transformation ofCaCl2

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SEMESTER II

MSCBT-204: GENETIC ENGINEERING

Unit I Core techniques in gene manipulation ; Cloning strategies ; Construction of-gene libraries; Probe construction; recombinant selection and screening -DNA sequencing, RFLP, DNA finger printing; Expression analysis; sequencing mutagenesis Engineering genes; Polymerase chain reaction; Ligase chain reaction. Unit II Expression systems and their applications; Production of protein from cloned genes; gene cloning in research, medicine and agriculture; Intellectual Property Rights (1PR) and patents, biosafety, containment facilities for Genetic Engineering experiments. Regulations on field experiments and release of GMO's (Genetically Modified Organisms), labelling of GM (Genetically Modified) Foods. ' Unit III E.Coli vectors - Plasmid biology - pBR 322 and its derivatives - gene markers. Cloning vectors for gramnegative bacteria & phage - filamentous phages - Cosmid - phasmid. Cloning in Gram - Positive bacteria Bacillus subtilis - transformation techniques. Unit IV Gene expression in eukaryotes - Cloning in Yeast Saccharomyces cerevisiae genetics - life cycle - types of vectors - gene expression system. Eukaryotic Vectors - SV40 - molecular genetics - markers - expression system. Cloning and gene expression in Streptomyces.

Specialised cloning vectors for cDNA - synthesis of specific RNA in vitro- selection of vectors for copy number - cloning promoters and Terminators.

Unit V Plant cloning vectors - Agrobacterium turnefaciens - Ti piasmids -molecular genetics and gene expression. Animal cell cloning; vectors - Baculoviruses - mammalian expression, vectors. retroviral vectors Expression vectors - vectors for fusion protein - promoters - translation proteases - gene fusion - secretion - modification and protein refolding -inducible - constitutive expression cassettes. Practical Q1. Q2. Study of transduction, transformation, conjugation in E.coli. Study of mutation is E.coli.

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SEMESTER II

MSCBT-205: PRACTICAL

Enzymology & Enzyme Technology Bioinformatics Molecular Biology ­ II Genetic Engineering Internal Assessment

- 20 Marks - 15 Marks - 20 Marks - 20 Marks - 25 Marks

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SEMESTER III Unit I

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MSCBT-301: Immunology

Immune response : Type of Immunity, Antigens and haptens. Anatomy of lymphoid organs; primary and secondary lymphoid organs. Immunoglobulin Structure, function and synthesis, memory cells lymphocyte differentiation. Cell of the immune system : Mononuclear cells and granulocytes, Lymphocytes and their subsets. Unit II Biology of complement systems : structure and function of MHC class I & II molecules. Antigen recognition and presentation, cell mediated immune responses. Hypersensitivity reactions; Immune suppression and Immune Tolerance immune disorders, Transplanation. Role of complement system in immune responses. Unit III Antigen - Isolation, purification and characterization of various antigens and haptens from pathogens and other biological molecules by biophysical and chemical and affinity separation methods. Production of antibodies, purification of antibodies, Quantitation of immunoglobulin by RID, EID and nephlometry. Hybridoma and monoclonal antibody production. Immuno-diagnosis and Applications of monoclonal antibodies in biomedical research; human monoclonal antibodies, Catalytic antibodies; complement fixation test; assessment of immune complex in tissues. Unit IV Purification of mononuclear from peripheral blood; isolation and characterization of T cell subsets; B cells and macrophages. Fluroescent Activated cell sorter (FACS); Mitogen and Antigen induced Lymphoproliferation assay. Cell mediated Lympholysis; Mixed Lymphocyte reaction. Assessment of delayed hypersensitivity reactions; Macrophage cultures. Assay for Macrophage activation; Isolation of dendritic cells; In situ and in vivo characterization of cells from tissues; Generation of T cell HLA typing. Unit V Cytokines : Structure and functions, Cytokine receptors, Biology and assay of cytokiness. Cytotoxic Tcells and thir mechanism of action, NK cells and mechanism of target cell destruction. Vaccine technology including DNA vaccines; identification of T & B epitopes for vaccine developments, Immunotechnology and infectious diseases. Immunoscreening of recombinant library. Practical

Ql. Blood film preperation and identification of cells. Q2.Seperation of serum proteins by electrophoresis. Q3.Study of antigen and antibody interaction by double diffusion method. Q4. Study of antigen and antibody interaction by immuno-electrophoresis. Q5. Study of antigen and antibody interaction by counter-current immuno-electrophoresis.

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SEMESTER III

MSCBT-302: Plant Biotechnology

Plant genome organisation, structure of representative plant genes and gene families in plants Organisation of chloroplast genome, nuclear encoded and chloroplast encoded genes for chloroplast proteins, targeting of oroteins to chloroplast - Organisation of mitochondria! genome - encoded genes for mitochondrial proteins - RNA editing for plant mitochondria -cytoplasmic male sterility - Seed storage proteins - maize transposabie elements, their organisation and function, transposabie elements in transgenic plants - Regulation of gene expression in plant development -Plant hormones and phytochrome. Unit II Symbiotic nitrogen fixation in legumes by Rhizobia - Nitrogen fixation in Cyanophyta. the biochemistry, molecular biology and gene rearrangement. Agrobacterium and crown gall tumors - Mechanism of TDNA transfer to plants, Ti plasmid vectors and its utility - Classification and molecular biology of plant viruses - Molecular biology of -plant stress response. Transgenic plants and applications vaccine and other biological developments. Unit III Genetic engineering in plants, selectable' markets, reporter genes and promoter used in plants by physical means - Genetic engineering of plants for construction of genome libraries and CDNA libraries, Molecular breeding.

Practical Preparation of fissure culture medium for plants Preparation of single cell suspension from spleen or thymus & plants. Protoplast fusion. Cell counting & cell viability . Callus Propagation, organogensis, transfer of plant to soil.

SEMESTER III

MSCBT-303: Environmental Biotechnology

Unit-I Environment: basic concepts and issues ; environmental pollution : types and methods for the measurement; methodology of environmental management- problem solving approach, its limitations; air pollution and its control through biotechnology, air sampling techniques; biodiversity : conservation and management. Unit - II Water pollution and its control: water as a scarce natural resource, need for water management, sources and measurement of water pollution, waste water treatment-physical, chemical and biological treatment processes; algal blooms and human health. Unit-III Microbiology of waste water treatments : aerobic process- activated sludge, oxidation switches, trickling filter, towers, rotating discs, rotating drums, oxidation ponds; anaerobic processes- anaerobic digestion, anaerobic filters, upflow anaerobic sludge blanket reactors; treatment schemes for waste waters of dairy, distillery, tannery industries; biotechnological applications of microbes from extreme environment. Unit-IV

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Microbial degradation of xenobiotics in the environment - ecological considerations, decay behaviour & degradative plasmids, hydrocarbons, substituted hydrocarbons, oil pollution, surfactants, pesticides; bioaccumulation of metals and radio-nuclerds and detoxification ; bioremediation. Unit-V Biological N2 fixation, H2 production, biofertilizers and biopesticides; solid wastes: sources and management (composting, wormiculture and methane production). Single cell protein (Spin/Una, yeast, mushroom); global environmental problems- ozone depletion, UV-B, green house effect and acid rain, their impact and. biotechnological approaches for Practical Isolation of industrially important microorganism for microbial processes. Determination of thermal death point (TDP) and thermal death time (TDT) of microorganism for design of a sterilizer. Comparative study of Ethanol production using different substrate .

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

SEMESTER III

MSCBT-304: Bioprocess Engineering & Technology

Unit-I Introduction to bioprocess engineering, bioreactors, isolation, preservation and maintenance of industrial microorganisms; kinetic of microbial growth and death; media for industrial fermentation, air and media sterilization. Unit - II Types of fermentation processes, analysis of batch, fed-batch and continuous bio-reactides, stability of microbial reactors, analysis of mixed microbial populations, specialized bioreactors (pulsed, fluidized, photobioreactors etc.); measurement and control of bioprocess parameters.

Unit - III Downstream processing: introduction, removal of microbial cells and solid matter, foam reparation, precipitation/filtration, centrifugation, cell disruptions, liquid-liquid extraction, chromatography; membrane process, drying and crystallization; effluent treatment- D.O.C. and C.O.D. treatment and disposal of effluents; whole cell immobilization and their industrial applications. Unit - IV Industrial production of chemicals: alcohol (ethanol), acids (citric acetic and gluconic) solvents, (glycerol acetone butenol) , antibiotics (penicillin), amino acids (lysine), single cell-protein.

Unit - V

Use of Microbes in mineral beneficiation and oil recovery; Introduction to food technology: Elementary idea of canning and packing, sterilization and pasteurization of food products, technology of typical food / food products( Bread, Cheese, Idli ), food Preservation.

Practical Isolation of antibiotic producing microorganism from soil. Use of alginate for cell immobilization.

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Detection of coli--for determination of purity of potable w--. Determination of COD/BOD for a sewage sample. Determination of the efficiency of removal of air. Pollutant by using air samples. Test for the degradation of aromatic hydrocarbon by bacteria.

SEMESTER III

MSCBT-305: Practical

Immunology Plant Bio-Technology Environmental Biotechnology Bioprocess Engineering & Technology Internal Assessment : 25 Marks : 20 Marks : 15 Marks : 20 Marks : 20 Marks

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SEMESTER IV

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

MSCBT-401: Biostatistics

Unit I:- Introduction:- definition, scope and limitation of biostatistics; concept of variables in biological systems; collection, classification, tabulation, graphical diagrammatic representation of data, Measures of central tendency (arithmetic, harmonic & geometric), median and mode; confidence limit of population mean. Unit II:- Measure of dispersion: range, standard deviation variance, coefficient of variation; definitionand basic properties of probability, normal and binomial distribution function, test of significance, hypothesis, error, level of significance; t-statistics: test that a population mean equals a specified value, paired 't' test. Unit III:- F-statistics: one way analysis of variance (sample size equal and unequal), chi-square statistics, test of goodness of fit, test of independence of factor; co-relation and its coefficient/ linear regression and its coefficient regression equation & its diagram. Unit IV:- Introduction to data base concept, introduction to internet and its application, introduction to MS office software, covering word processing, spreadsheets, introduction to hardware graphics. Unit V:- Computer oriented statistical techniques by using Excel; frequency table of single discrete variable, computation of mean, variance and standard deviation;

SEMESTER IV

MSCBT-402: Animal Biotechnology

Unit - I Basic principles of Biotechnology as applicable to animal Science Artificial Insemination, pregnancy diagnosis, In-vitro fertilization - Embryo Transfer Technology - Transgenic Animals. Unit - II Animal Cell culture - Applications in Animal biotechnology. Bio hazard and Biosecurity in Animal BiotechnologyEthical aspects in Animal Biotechnology. Unit - III Biofueling and Control and development technology. Bioremediation. Use transgenic technology to study fish growth

Unit IV Genetic engineering and ploidy manipulation to enhance grow reproduction and development of disease resistance in aquaculture species. Cryobiology in Marine germplasm preservation Pharmaceuticals from marine organisms.

SEMESTER IV

MSCBT-403: Pharmaceutical Biotechnology

Unit I Production recombinant pharmaceuticals : Recombinant insulin Human Growth factor synthesis Recombinant factor VIII Unit II

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Synthesis of : Recombinant vaccine Recombine Protein Live recombinant vaccine Unit III Identification of genes responsible for human disease. Breast cancer Autosomal dominant and recessive disorders. X-linked disorders. Unit IV Gene therapy of somatic cell. Genetic Manipulation of Grem cell. Unit V Prospects and Ethics of gene therapy

SEMESTER IV

MSCBT-404: Research Methodology

Introduction to Research: Definition, Scope, Limitations, and Types. Objectives of Research Research Process Research Designs Data Collection: Secondary Data, Primary Data, and Methods of Collection. Scaling Techniques: Concept, Types, Rating scales & Ranking Scales Scale Construction Techniques, Multi Dimensional Scaling. Sampling Designs: Concepts, Types and Techniques Sample Size Decision Theory of Estimation and Testing of Hypothesis Small & Large Sample Tests, Tests of Significance based on t, F , Z test and Chi-Square Test. Designing Questionnaire. Interviewing. Tabulation, Coding, Editing. Interpretation and Report Writing.

SEMESTER IV

MSCBT-405: Practical / Training

Biostatistics Animal Biotechnology Pharmaceutical Biotechnology Research Methodology Internal Assessment: 25 Marks

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: 20 Marks : 20 Marks : 15 Marks : 20 Marks

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M.Sc. (Bioinformatics) (MSCBI)

SEMESTER I MSCBI-101: Bioinformatics- 1

· Computational analysis of protein sequence, structures and function. Sequence and structure databases, visualization and modeling tools. Methods for pairwise alignment, discovery and searching for remote homologous in protein sequence databases. Construction of profile Hidden Markov models, including sequence weighting and Dirichlet mixture priors. Multiple alignment of protein sequences. The prediction of secondary structure by neural networks, hidden Markov models, and other methods. Analysis of X-ray, NMR and mass spectrometry data. Models of protein structure. Protein threading methods. Computational methods for structure-structure alignment and prediction of function from structure. Construction of protein structure models by homology. Ab initio prediction of protein structure. Molecular dynamics models of protein structure and folding.

·

· ·

SEMESTER I MSCBI-102: Biochemistry-I

· · · · · A general overview for graduate and advanced undergraduate students in agricultural, biological, chemical and nutritional sciences. Chemistry of amino acids, proteins, carbohydrates, lipids, vitamins and protein structure; Enzymology; carbohydrate metabolism. Metabolism of carbohydrates, amino acids nucleotides and lipids; biosynthesis and molecular relationships among DNA, RNA and proteins; Genetic code. Regulation of gene expression; selected topics in the molecular physiology of plants and animals.

SEMESTER I MSCBI-103: Machine Learning

· · Algorithmic models of learning. Design, analysis, implementation, and applications of learning algorithms. Learning of concepts, classification rules, functions, relations, grammars, value functions, models, skills, behaviors and programms. Agents that learn from observation, examples, instruction, induction, imitation, deduction, reinforcement, and interaction. Computational learning theory. Data mining and knowledge discovery using artificial neural networks, decision trees, Bayesian learning, association rules, genetic algorithms, dimensionality reduction, feature selection, and visualization. Learning from heterogeneous, distributed, dynamic data and knowledge sources. Learning in multiagent systems. Selected applications in automated knowledge acquisition, pattern recognition, programm synthesis, bioinformatics, and interest-based information systems.

·

· ·

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SEMESTER I MSCBI-104: Theory of Statistics and Genetic Statistics

· · · · · Point estimation including maximum likelihood estimation, Bayes estimators, Bayesian and minimax optimality, unbiasedness, sufficiency, completeness, Basu's theorem. Convergence in probability, convergence in distribution, laws of large numbers, central limit theorm. Confidence intervals, prediction intervals; Hypothesis testing, Neyman-Pearson Lemma, uniformly most powerful tests, likelihood ratio tests. Bayesian interval estimation and tests. Nonparametric methods, bootstrap. Probability applied to genetic systems; random matin; selection, mutation and migration; theory of in breeding; effects of finite population size; basic concepts in quantitative genetics; prediction of progress from artificial selection. Sampling designs and experimental designs to obtain information from markers; detecting major genes; linkage analysis and segregation analysis; finding alignments and similarities between DNA sequences; constructing phylogenetic trees.

·

SEMESTER I MSCBI-105: Communication and Soft Skills

UNIT I Essentials of Grammar: · · · · Parts of Speech Punctuation Vocabulary Building Phonetics

UNIT II

Office Management: · · · · · · · UNIT III Letter & Resume Writing: · · · · · · · UNIT IV

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Types of Correspondence Receipt and Dispatch of Mail Filing Systems Classification of Mail. Role & Function of Correspondence MIS Managing Computer

Types of Letters-Formal / Informal Importance and Function Drafting the Applications Elements of Structure Preparing the Resume Do's & Don'ts of Resume Helpful Hints

Presentation Skills: · · · · · · · · · · · UNIT V Interview Preparation: · · · · · · · · UNIT VI Group Discussion & Presentation: · · · · · Definition Process Guidelines Helpful Expressions Evaluation Types of Interview Preparing for the Interviews Attending the Interview Interview Process Employers Expectations General Etiquette Dressing Sense Postures & Gestures

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

Importance of Presentation Skills Capturing Data Voice & Picture Integration Guidelines to make Presentation Interesting Body Language Voice Modulation Audience Awareness Presentation Plan Visual Aids Forms of Layout Styles of Presentation.

(Note: Every student shall be given 15 minutes. of presentation time & 45 minutes of discussion on his/ her presentation.) The student will be evaluated on the basis of : his / her presentation style Feedback of Faculty & Students General Etiquette Proficiency in Letter Drafting / Interview Preparation The paper is internal and at least 3 tests will be taken. Best 2 of 3 shall account for final grades (70% Test & 30% Presentation)

SEMESTER I MSCBI-106: Practical

Bioinformatics ­ 1 Biochemistry ­ 1 Machine Learning Theory of Statistics and Genetic Statistics Internal Assessment: 25 Marks

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- 20 Marks - 20 Marks - 20Marks - 15 Marks

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SEMESTER II MSCBI-201: Bioinformatics- 2

· · · Genomics databases and analysis of high-through put genomics data sets including genomic DNA, ESTs, SNPs, DNA micro array expression data, yeast 2-hybrid and other methods. BLAST and related sequence comparison methods. Pairwise alignment of biosequences by dynamic programming. Using Gibbs Sampling, expectationmaximinization, and other statistical methods to discover common motifs in biosequences. Multiple alignment and database search using motif models. · · · · Methods for constructing phylogenetic trees. Hidden Markov models for finding genes and other genomic features. Discriminative methods for the analysis of bioinformatics data including generalized linear models, neural networks and support vector machines. Methods for locating genes and predicting gene function, including introduction to linkage analysis and disease association studies using SNPs. Methods for modeling the structure of DNA and RNA, including stochastic context-free grammars and pseudo-energy minimization methods.

SEMESTER II MSCBI-202: Biochemistry ­ 2

· · · · · · · ·

Chemical composition of living matter. Chemical composition of amino acids, proteins, carbohydrates and lipids. Enzymology and co-enzymes. Metabolism of carbohydrates and biological oxidation. Metabolism of lipids, aminoacids, and nucleotides and membrance biochemistry. Biosynthesis of DNA, RNA and proteins; gene regulation; (selected topics). Structure and properties of Reactivity Biomolecules. Physical Biochemistry. Selected topics in physical chemistry in the context of applications to problems in biology, biochemistry and food sciences. Not acceptable for credit toward a major in biochemistry or biophysics.

· ·

Protein Chemistry-Physical Methods. Protein structure determination as a means of understanding biological function.

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SEMESTER II MSCBI-203: Design and Analysis of Algorithms

·

Basic techniques for design and analysis of efficient algorithms. Sorting, searching, graph algorithms, computational geometry, string processing and NP-completeness. Design techniques such as dynamic programming and the greedy method.

· · · · ·

Asymptotic, worstcase, average-case amortized analyses. Data structures including heaps, hash tables binary search trees and red-black trees. Programming projects. A study of basic algorithm design and analysis techniques. Advanced data structures, amortized analysis and randomized algorithms. Applications to sorting, graphs, and geometry. NP-completeness and approximation algorithms

SEMESTER II MSCBI-204: Database Systems

· · · · · ·

Introduction to database concepts. Data models. Algebraic, first order calculus based, and user oriented query languages. Data storage, access methods, query execution, and transaction management. Parallel and distributed databases. Special purpose databases. Information integration using data warehouses, mediators, wrappers, and data mining. Oral and written reports.

SEMESTER II MSCBI-205: Practical

Bioinformatics ­ 2 Biochemistry ­ 2 Design and Analysis of Algorithms Database Systems Internal Assessment: 25 Marks - 20 Marks - 20 Marks - 15 Marks - 20 Marks

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SEMESTER III MSCBI-301: Introduction to Data Structure for Biologists

· ·

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

An object-oriented approach to programming and data structures for biologic. Object-oriented programming. Strings. Stacks. Queues. Recursion. Lists. Trees. Graphs. Sorting. Algorithm Analysis. The course is designed to provide the fundamentals of data structures and programming for biology students that already have basic programming skills.

SEMESTER III MSCBI-302: Bio-computing and Computational Biology

· Students will become familiar with use of arrange of programming skills useful for writing bioinformatics software. They will gain experience of work in small groups and of linking together different types of program to form a software package. · · The UNIX operating system; PERL programming; Web authoring; relational databases; middle ware; Corba. Assessment -Demonstration of software resulting from a group programming project. Introduction to essential statistical and computational methods and tools in molecular biology research. Emphasis will be on concepts and principles, combined with hands on (keyboard) applications. · Topics include : molecular databases, score-based sequence analysis, amino acid substitution scoring matrices, query search problems, dynamic programming and other methods for pairwise sequence alignment, motif identification, multiple sequence alignment, construction of phylogenetic trees from sequence data, gene structure prediction, protein structure prediction, and selected current research topics.

SEMESTER III MSCBI-303: Software Engineering

· · · Introduction to the general principles of software engineering. Covers current and classical topics from both practical and theoretical viewpoints. Topics include software evolution, project management, software inspections, design methods, requirements analysis and specification, software testing, maintenance, software implementation, human interfaces, and software engineering experimentation.

SEMESTER III MSCBI-304: Pharmacology

· · · · · Drug metabolism & Pharmacogenetics. Frontiers in Pharmacology Statistical methdos in Pharmacology Introduction to Research in Pharmacology. Introduction to Pharmacoinformatics

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SEMESTER III MSCBI-305: Practical

Introduction to Data Structure for Biologists Biocomputing and Computational Biology Software Engineering Pharmacology Internal Assessment: 25 Marks

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- 20 Marks - 20 Marks - 20 Marks - 15 Marks

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SEMESTER IV MSCBI-401: Introduction to Java and Introductory Combinatorics

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

To introduce students to a modern programming language and help them gain sufficient fluency to undertake research projects with a programming component; to lay the foundations for more advanced study of object-oriented languages. Objectives -to understand the basic concepts of programming; to learn the syntax and semantics of Java; to be able to use a program development environment; to become familiar with some fundamental algorithms and data structures. Variables; arithmetic; input and output; operators and control statements; functions; arrays and pointers; structures and strings; dynamic memory allocation; object-oriented programing. Practical : Students will be expected to work their way through a set of pogramming exercises designed to demonstrate various aspects of Java and object-oriented programming. Permutations, combinations, binomial coefficients, inclusion-exclusion principle, discrete probability, classical probability. Additional topics selected from recurrence relations, generating functions, random walks, and Markov chains.

SEMESTER IV MSCBI-402: Basic Research Skills

Objectives : This module is designed to provide a general appreciation of workplace and communication skills pertinent to the biological and medical sciences and an understanding of some of the legal constraints and requirements of biological/medical research. Inter-personal and personal transferable skills will be given particular emphasis in an effort to better equip the student for the workplace, as well as interaction with staff and students while attending their Masters course. Assessment - Written essary presenting a topic related to Bioinformatics to a non-specialist reader. Talk at a Student Symposium presenting an area of Bioinformatics to the rest of the class and other members of the school.

SEMESTER IV MSCBI-403: Computational Techniques for Genome Assembly and Analysis

This course focuses on practical sequence assembly and comparison techniques. It has a special emphasison efficient techniques for processing raw and finished DNA sequences at the genome level. Those techniques are increasingly important, as complete sequences are becoming available for animal and plant genomes. Algorithms and software for assembly and analysis of genomes. Global alignment, local alignment, overlapping alignment, baded alignment, linear-spaco alignment, word hashing, DNAprotein alignment, DNA-cDN Aalignment, comparisation of two sets of sequences, construction of contigs, generation of consensus sequences.

Paramedical Syllabus Manual : 143 / 144

PTU/DEP/Paramedical/September 2005/Detailed Syllabus

SEMESTER IV MSCBI-404: Proteomics

· · · · · · · · Protein Sequence Comparison Principles of Protein Structure Prediction Protein Multiple Sequence Alignment Automatics Structural Classification of Proteins Protein Structural Comparison Geometric Hashing, Rigid and Flexible Matching Protein Folding Methods of resolving Protein 3D Structures

SEMESTER III MSCBI-505: Practical

Introduction to Java and Introductory Combinatorics Basic Research Skills Computational Techniques for Genome Assembly and Analysis Proteomics Internal Assessment: 25 Marks - 20 Marks - 20 Marks - 20 Marks - 15 Marks

Paramedical Syllabus Manual : 144 / 144

September 2005

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