Read Front Cover:Front Cover text version

2009 Catalog

Shakers, CO2 Incubators, Freezers, Fermentors, Bioreactors and more

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Exciting New Developments from NBS

Dear Reader, Welcome to the 2009 edition of New Brunswick Scientific's life science equipment catalog. We have many exciting new products and changes to tell you about. Introducing NBS Galaxy® CO2 Incubators - We are pleased to introduce this new line of advanced CO2 incubators, ideally suited for traditional cell culture requirements, as well as today's more sophisticated protocols. These units are packed with even more standard features and options than our previous models, including new active humidification control and an improved cooling option. For details, see page 28. New Space-Saving Incubator Shakers - For labs short of space, we've developed two new incubator shakers that fit on or under your lab bench, or can be stacked two high on the floor. Innova 42 and 42R shakers provide programmable controls for set-it and forgetit convenience, as well as include a stationary culture shelf and internal power receptacle for exceptional versatility. You can learn more about them on page 8. New Data Logging Software for Shakers, Freezers & Incubators - BioCommand® SFI for shakers, freezers and incubators provides advanced remote logging and control of your NBS equipment in a convenient software package. It's described on page 32. "Green" Initiatives - NBS has been at the forefront of green initiatives long before being "green" came into vogue. We not only recycle everything from scrap metal to toner cartridges, paper and plastics, but we build our freezers to be 95% recyclable by weight so you can recycle our products too. And, while typical freezers can be among the most energy-hungry pieces of equipment in the lab, our freezers are among the most energyefficient models in the market today. We now also offer a new line of hydrocarbon-based freezers to further reduce the carbon footprint. They are described on page 21. Eppendorf Products Showcase - Last but not least, as a member of the Eppendorf family, we are proud to include a sampling of Eppendorf's complementary portfolio of life science products in our 2009 catalog. While their equipment is offered through Eppendorf sales offices, we invite you to view their pipettes, centrifuges, and more on pages 34 to 37. From all of us at New Brunswick Scientific, thank you for your continued loyalty to our products and services. We appreciate the opportunity to serve you. With warm regards,

Jim Orcutt, President & CEO New Brunswick Scientific

a culture of innovation

New Brunswick Scientific · [email protected] · www.nbsc.com · 800.631.5417 · Fax: 732.287.4222

Expanding the Cell Culture Universe

Introducing Galaxy® CO2 Incubators

Whether you are applying traditional cell culture techniques, or experimenting with innovative new protocols, these redesigned CO2 incubators from New Brunswick Scientific provide the advanced features and options you require to progress your science to the next level.

· New Active Humidification Option ideal for microplate and low volume cell culture. · New UV Sterilization Option for active protection from contamination. · New Cooling Option for better performance and wider temperature range. · New Redesigned Sealed Inner Glass Door is now standard on CO-170 models, resulting in improved recovery, uniformity, and reduced CO2 consumption. · New Perforated Shelving for faster humidification recovery and improved uniformity. · New Economy Model 3.6 cu.ft. (48L). · Access Port and RS232 Port now standard on all units.

For details, see pages 28 - 31

By combining these new features with a unique direct heating platform, three ranges of O2 control, a high temperature decontamination option, and a large usable volume, we are sure that the Galaxy line will suit your laboratory for years to come.

a culture of innovation

Table of Contents

PAGE

Ordering Information & Warranty . . . . . . . . . . . . . . . . . . . .2 Shakers & Accessories Shaker Selector Chart . . . . . . . . . . . . . . . . . . . . . . . . . . .3 . . . . . . . . . . . . . . . . .4 CO2 Incubators (continued) Incubator Options & Accessories . . . . . . . . . . . . . . . . . .31 Shaker, Freezer & CO2 Incubator Control BioCommand SFI Automated Data Logging & Control - New! . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32 Roller Drums Tissue/Microbial Culture Roller Drum, TC-7 & TC-8 . . . . . .32 Media Sterilizer & Plate Pourer MM9 MediaMatic® Benchtop Media Sterilizer . . . . . . . . . .33 MP-1000 PourMatic® Plate Pouring System . . . . . . . . . . .33 Eppendorf Products Showcase . . . . . . . . . . . . . . . . . . .34 - 37 Fermentation & Cell Culture Contract Lab Services & Training . . . . . . . . . . . . . . . . .38

PAGE

Shaker & Platform Capacity Chart

Flask Clamps, Test Tube Racks & Microplate Carriers . . .5 Innova® Incubated / Refrigerated Shakers Innova 44 & 44R Stackable Floor Models . . . . . . . . . . . . . . .6 Innova 43 & 43R Console Models . . . . . . . . . . . . . . . . . . . .7 Innova 42 & 42R On/Under Bench or Stackable - New! .8 & 9 Innova 40 & 40R Benchtop Models . . . . . . . . . . . . . . . . . . .9 Innova Water Bath Shaker Innova 3100 Water Bath Model

. . . . . . . . . . . . . . . . . . . .10

Innova Open Air Shakers Innova 2000 & 2050 Compact Benchtop . . . . . . . . . .11 - 12 Innova 2100 & 2150 Mid-Size Benchtop . . . . . . . . . . .11 - 13 Innova 2300 & 2350 Large-Capacity Benchtop . . . . .11 & 13 Innova 5000 Large-Capacity Multi-Tier Shaker . . . . . . . . . .14 Innova 5050/5051 Racking System . . . . . . . . . . . . . . . . . . .14 I Series Incubated / Refrigerated Shakers I26 & I26R Stackable Floor Models . . . . . . . . . . . . . . . . . .15 Excella® Incubated / Refrigerated Shakers E24 & E24R Benchtop Models . . . . . . . . . . . . . . . . . . . . . .16 E25 & E25R Console Models . . . . . . . . . . . . . . . . . . . . . . .17 Excella Open Air Shakers E10 Large-Capacity Benchtop . . . . . . . . . . . . . . . . . . . . . .17 E5 Mid-Size Benchtop . . . . . . . . . . . . . . . . . . . . . . . . . . . .18 E1 & E2 Compact Benchtop . . . . . . . . . . . . . . . . . . . . . . . .18 C-Line Water Bath Shaker C76 Large Capacity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19 Freezers, Ultra-Low Temperature (-86°C) Five Considerations In Selecting a Freezer . . . . . . . . . . . .20 About NBS Energy-Saving Eco-Friendly Freezers . . . . . . . .21 Hydrocarbon-Based "Green" Models . . . . . . . . . . . . . . . . . .21 Innova U725-G & Premium U570-G - New! . . . . . . . . . . . .21 Innova Freezers with Vacuum Insulation Panels Innova U101, U360, U535 & U725 Upright Models . . .22 - 23 Innova C585 & C760 Chest Models . . . . . . . . . . . . . . . . .22 - 23 Premium Freezers with Traditional Insulation Premium U410 & U570 Models. . . . . . . . . . . . . . . . . . . . . . . . . . . 24 Premium C340 & C660 Chest Models . . . . . . . . . . . . . . . . . . . . 24 Accessories, All Freezer Models . . . . . . . . . . . 23 & 25 - 27 Independent Temperature Monitoring System - New! . .27 CO2 Incubators NBS Galaxy® Direct-Heat Incubators . . . . . . . . . . . . .28 Galaxy Models CO-170R & CO-170S - New! . . . . . . . . . . .29 Galaxy Models CO-48R, CO-48S & CO-14 - New! . . . . . . .30

Fermentor/Bioreactor Selector Chart . . . . . . . . . . . . . . .39 FibraCel® Disk Solid-Support Growth Matrix . . . . . . .40

Disposable Systems FibraStageTM Cell Culture System, 500 mL . . . . . . . . . . . . .41 Automated Cell Counting Systems NucleoCounter® for Mammalian Cells & Yeast . . . . . . . . . . . . .42 Growing CHO Cells, one in a series of guides . . . . . . . .43 Autoclavable Benchtop Fermentors & Bioreactors BioFlo® 110 Fermentor/Bioreactor, 1.3 - 14 L . . . . . . . . . .44 - 49 BioFlo 310 Fermentor/Bioreactor 2.5 - 14 L . . . . . . . . . . . .50 - 51 BioFlo 3000 Upgrade to a BioFlo 310 . . . . . . . . . . . . . . . . . . .51 Impellers, Which is Best for Your Cell Line . . . . . . . . . . . . . .52 RPC Reactor Process Controller . . . . . . . . . . . . . . . . . . . . . . .53 Autoclavable Benchtop Fermentors & Bioreactors cont'd. CelliGen® 310 Dedicated Bioreactor, 2.5 - 14 L . . . . . . . . . . . . . 54 - 56 CelliGen Plus Upgrade to CelliGen 310 . . . . . . . . . . . . . . .55 & 57 Sterilizable-in-Place Fermentors & Bioreactors BioFlo 415 Fermentors, 7 - 19.5 L . . . . . . . . . . . . . . . . . . . .. 58 BioFlo & CelliGen 510 Fermentors/Bioreactors, 19.5 - 40 L- New! . . . . . . . . . . . . . . . . . . . . . . . . . . . . .59 BioFlo Pro Fermentors, 75 - 3000 L . . . . . . . . . . . . . . . . . .60 - 61 CelliGen Pro Bioreactors, 75 - 650 L . . . . . . . . . . . . . . . .60 & 62 Accessories, Fermentors & Bioreactors Pumps, Connectors, Tubing & Chillers . . . . . . . . . . . . . . . .63 Additon Vessels, Sample Vials & Chart Recorders . . . . . . .64 Controllers & EX-2000 Off-Gas Analyzer . . . . . . . . . . . . . .65 BioCommand® Software including OPC Packages - New! . .66 Probes/Sensors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67 - 70 CEPA High-Speed Centrifuges . . . . . . . . . . . . . . . . . . . . . .71 Service Plans to Protect Your Investment . . . . . . . . . . . . . .72 Index By Catalog Number . . . . . . . . . . . . . . . . . . . . . . .73 - 74

1.732.287.1200 · 1.800.631.5417 · Fax: 1.732.287.4222 · [email protected]

1

NBS Ordering & Warranty Information

TO PLACE ORDERS WARRANTY

New Brunswick Scientific's equipment is protected by a comprehensive warranty and on-site service in your facility. The warranty covers faulty components and assembly, and our obligation under this warranty is limited to repairing or replacing the instrument or part thereof, which shall prove to be defective after our examination. The NBS warranty does not cover loss of time or materials, such as the loss of biological or biochemical by-products caused by any work interruption resulting from equipment failure; it does not extend to equipment that has been subject to misuse, neglect, accident or improper installation or application; nor does it cover any machine that has been repaired or altered outside the NBS factory without prior authorization from NBS. Expendable items such as lamps, probes, sensors including incubator sensors, glass, filters, etc., are not covered. The warranty begins on the date the equipment ships and extends for a period as per the following: Instrument SHAKERS: Innova Shakers I Series Shakers Excella Shakers & C-76 Shakers Shakers Accessories* CO2 INCUBATORS: Incubator Accessories ULT FREEZERS: Parts Warranty 3 years 2 years N. America: 2 years Elsewhere: 1 year 1 year 2 years 1 year 5 years VIP panels: 12 years 1 year Labor Warranty 2 years 2 years N. America: 2 years Elsewhere: 1 year 1 year 2 years 1 year 2 years 1 year

New Brunswick Scientific (NBS)

P.O. Box 4005, 44 Talmadge Road Edison, New Jersey 08818-4005 USA CUSTOMER SERVICE PHONE: N. America: 1.800.631.5417, 2 · Elsewhere: 1.732.287.1200, 2 FAX: N. America: 1.800.489.1400 · Elsewhere: 1.732.248.0589 E-Mail Orders: [email protected] Specify: · Model number/name · Catalog number · Electrical service Provide: · Name of person ordering equipment · Complete shipping address · Building name · Department and room number where equipment is to be located Orders will be promptly acknowledged via e-mail, indicating the date of anticipated shipment, and price.

PRODUCT SPECIFICATIONS

Specifications of the products described in this catalog are subject to change without notice. Such changes may result from our long-standing commitment to improve performance, and extend the service life and applications of our products. NBS may change product design and specifications at any time without incurring obligation. To ensure that the equipment meets individual requirements, up-to-date specifications will be incorporated in formal quotations to afford you a means for final review and approval of the equipment before making a purchase. Photos shown are for illustration purposes and are not shown to scale.

Freezer Accessories FERMENTORS, BIOREACTORS & ALL OTHER NBS EQUIPMENT:

1 year

1 year

(*) Chart recorders, photosynthetic light banks, etc. () Stacking stand, casters, shelves, etc. () Chart recorders, CO2/LN2 back up systems, etc.

SHIPPING

Freight charges are added to invoice, unless you specify otherwise. Orders are shipped via best method, or you may designate a specific carrier, mode of shipment, and/or inside delivery to a location other than the receiving dock. Please also specify if your loading dock requires a truck with lift gate.

EXTENDED WARRANTY OPTION

To minimize downtime from unexpected malfunctions in equipment operation, NBS offers one and two year extended warranties, as well as programs customized to your specific requirements. For details, contact our Repair Service Department at: 1.732.287.1200, 3 or 1.800.631.5417, 3 prior to the expiration of your original factory warranty.

SPARE PARTS KITS

To eliminate downtime, a supply of expendable replacement parts should be available at all times for uninterrupted operation of major equipment. Most products are available with optional kits of recommended spare parts to make expendable components immediately available should you need them. The kits are listed under "Accessories" for each product. Standard kits contain parts such as fuses, belts, O-rings, gaskets, membranes, filters, motor brushes, ball bearings, etc. More comprehensive kits contain a larger number of spare parts, including bearing housings, valves, motors and circuit boards. Each kit varies, depending on the equipment's electrical service and year of manufacture. For a description, contact Customer Service (as shown above) and specify equipment serial number and electrical service.

INTERNATIONAL SHIPMENTS

NBS sells and supports our products worldwide through subsidiary offices as well an international network of distributors. For equipment with an ultimate destination outside the U.S., consult our Customer Service Department for the name of the NBS sales representative in your area.

EQUIPMENT RETURNS

If there is a need to return equipment, first contact our Customer Service Department to obtain a Return Material Authorization (RMA) number. Please specify instrument model, serial number and original purchase order number. Equipment ordered may be authorized for return to the factory for a period not to exceed 90 days from the date of shipment. However, depending on condition, there may be a restocking charge. Material will not be accepted without such authorization, and the RMA number must be clearly displayed on each of the containers in the return shipment.

WARRANTY REGISTRATION

To register your warranty with NBS, complete the on-line form at www.nbsc.com under the "How Can We Help?" tab, or use the warranty card enclosed with your equipment.

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New Brunswick Scientific · a culture of innovation · www.nbsc.com

Shaker Selector Chart

New Brunswick Scientific is the leading supplier of biological shakers, with over two dozen models offered to suit a wide variety of applications and budgets. Advanced Innova® & I26 Shakers: Innova and I26 Shakers feature the NBS heavy-duty, Triple-Eccentric Drive, providing uniform motion and superb reliability. These models handle heavier workloads than our Excella line, feature a broader temperature range, and provide better control. For labs short on space, we offer several space-saving stackable models. Many Innova incubator shakers also provide advanced capabilities, such as gassing, humidity monitoring, and ability to automate multiplesetpoint changes on a timed basis. Economical Excella® Shakers: These shakers feature microprocessor controls and UniCentric counterbalanced drives for accurate control and dependable operation for most lab applications. For High-Temperature Water Bath Applications, Innova 3100 and C76 shakers are offered. Interchangeable Platforms (sold separately): Universal Platforms accommodate a mix of different size glassware on a single platform, providing maximum versatility. Clamps, Test Tube Racks, Sticky Pad and other accessories are sold separately. If using a single flask size only, our Dedicated Platforms include clamps pre-installed, and maximize platform capacity. See page 4. NEW for most models, BioCommand SFI enables programmable control and automated data logging for most incubated shakers. See page 32.

Siz e( Inc Ma he xN s) on um Un ber ive o rsa f 250 lP Ca lat mL F for l pa m asks Siz city e o of n U La niv rge ers st F Or al l bit Pla ask Dia tfo (in rm ch met es er ) Te m (A pera =A t N.A mb ure .=N ient Ran ge ot °C Ap pli ca ble RP ) M Ra ng e

Innova 2000 Innova 2050 Innova 2100 Innova 2150 Innova 2300 Innova 2350 Innova 3100 Innova 40 Innova 40R Innova 42 Innova 42R Innova 43 Innova 43R Innova 44 Innova 44R I26 I26R

13 x 11 16 x 12 18 x 18 24 x 18 30 x 18 36 x 24 12 x 16.5 18 x 18 18 x 18 18 x 18 18 x 18 30 x 18 30 x 18 30 x 18 30 x 18 30 x 18 30 x 18 6 platforms Innova 5000 32.5 x 26.8 13 x 11 13 x 11 18 x 18 30 x 18 18 x 18 18 x 18 30 x 18 30 x 18 12 x 16.5

6 10 18 24 30 50 8 18 18 18 18 30 30 30 30 30 30 252 6 6 18 30 18 18 30 30 8

4 x 500ml 8 x 500ml 2 x 6L 3 X 6L 4 x 6L 8 x 6L 2 x 2L 4 x 3L 4 x 3L 2 x 6L 2 x 6L 4 x 6L 4 x 6L 6 x 5L 6 x 5L 6 x 2.8L 6 x 2.8L refer to catalog 4 x 500ml 4 x 500ml 2 x 6L 4 x 6L 5 x 2L 5 x 2L 4 x 6L 4 x 6L 2 x 2L

0.75 0.75 0.75 0.75 1.0; 2.0 1.0; 2.0 0.5 0.75 ; 1.0 0.75 ; 1.0 0.75 ; 1.0 0.75 ; 1.0 1.0; 2.0 1.0; 2.0 1.0; 2.0 1.0; 2.0 1 1 2 0.75 0.75 0.75 1 0.75 0.75 1 1 0.5

N.A. N.A. N.A. N.A. N.A. N.A. A+5 to 100 A+5 to 80 A-15 to 80 A+5 to 80 A-20 to 80 A+5 to 80 A-20 to 80 A+5 to 80 A-20 to 80 A+5 to 60 A-15 to 60 N.A. N.A. N.A. N.A. N.A. A+7 to 60 A-15 to 60 A+7 to 60 A-15 to 60 A+7 to 80

25 to 500 25 to 500 25 to 500 25 to 500 25 to 500* 25 to 500* 25 to 400 25 to 500 25 to 500 25 to 400 25 to 400 25 to 500* 25 to 500* 25 to 400* 25 to 400* 25 to 400 25 to 400 25 to 350 50 to 400 50 to 400 50 to 400 50 to 400 50 to 400 50 to 400 50 to 400 50 to 400 50 to 300

Benchtop Benchtop Benchtop Benchtop Benchtop Benchtop Water bath Benchtop Benchtop

S

On/Under Bench; Floor Stackable (2 units) S Console Console S Floor stackable (up to 3) Floor stackable (up to 3) S Floor stackable (up to 3) Floor stackable (up to 3) S Three tiers large scale Benchtop (analog) Benchtop Benchtop Benchtop Benchtop Benchtop Console Console Water bath

Excella E1 Excella E2 Excella E5 Excella E10 Excella E24 Excella E24R Excella E25 Excella E25R C76

S S

B = Built In. O = Optional using BioCommand SFI software - see page 32. F = Factory installed option (*) Maximum speed for 2" orbit is 300 RPM () When stacked, maximum speed is 400 rpm with 250/500 mL flasks; maximum 300 rpm or lower with larger flasks.

1.732.287.1200 · 1.800.631.5417 · Fax: 1.732.287.4222 · [email protected]

Re frig era tio Mu n ltiSte Ph pP oto ro Ga synth gram ssi m e UV ng M tic Li ing gh Ge ani t Hu rmic fold s ida mid lL ity Mo amp Pag nit e or

B B B B B B B B O O F F F F F F F F F F F F F F F F F

Pla tfo rm

Ty pe of Sh ak er

Mo de l

11 11 11 11 11 11 10 9 9 8 8 7 7 6 6 15 15 14 18 18 18 17 16 16 17 17 19

O O O O

3

Shaker & Platform Capacity Chart

Universal and Dedicated Platforms

Each NBS shaker accepts a wide variety of interchangeable platforms.

Universal Platforms (U) have multiple holes enabling you to mount an assortment of flask clamps or other accessories on a single platform.The capacities shown below represent the maximum number of flasks in a given size that will fit on the platform in a balanced pattern. Universal Platform, clamps & accessories are sold separately. Dedicated Platforms (D) - When just one size flask will be used on the shaker, (ie. 250 mL flasks) Dedicated Platforms (D) come with flask clamps already mounted. Dedicated Platforms generally will hold a greater number of flasks than the Universal Platform, but do not offer the versatility.

For platform catalog numbers, see the corresponding shaker page. Clamps and test tube racks are shown on page 5.

Shaker Model, Platform Dimensions & Maximum Capacity Innova Models 2000 2050 2100 40/40R 42/42R 4000/4080 4200/4230 E5/E24/E24R 2150 4900 43/43R 2300 4300 Series 4400 Series 2350 3100 44/44R 5000

(Per platform Max. 6 per shaker)

Excella Models

E1/E2

E10/E25/E25R C76

I26 & C76

Models Platform (in & cm)

Ded Plat. (D) Univ Plat. (U) 10 mL 25 mL 50 mL 125 mL 250 mL 500 mL 1L 2L 2.8 L 4L 5L 6L

Large TT Rack Medium TT Rack Small TT Rack Microplate Rack (stack) Microplate Rack (1 layer)

I26

13" x 11" 33 x 28 cm D 60 32 20 12 8 6 N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A U 60 20 15 11 6 4 N/A N/A N/A N/A N/A N/A N/A N/A 3 3 N/A

16" x 12" 41 x 31 cm D 86 48 35 20 12 8 6 3 N/A N/A N/A N/A N/A N/A N/A N/A N/A U 86 32 32 16 10 8 N/A N/A N/A N/A N/A N/A N/A 3 4 6 N/A

18" x 18" 46 x 46 cm D N/A N/A 64 34 25 16 9 5 4 4* 4 2* N/A N/A N/A N/A N/A U 109 64 45 21 18 14 8 5 4 4* 4* 2* 4 5 5 8 2

24" x 18" 61 x 46 cm D N/A N/A 80 48 35 20 12 8 5** 4** N/A** 4** N/A N/A N/A N/A N/A U N/A N/A 63 35 24 20 12 6 5 5 4 3 6 7 8 12 3

30" x 18" 76 x 46 cm D N/A N/A 108 60 40 24 15 12 6 6 N/A 4 N/A N/A N/A N/A N/A U 183 92 92 39 30 18 12 8 6 6 6 4 7 9 9 16 4

36" x 24" 91 x 61 cm D N/A N/A N/A 96 70 40 24 15 12 12 N/A 6 N/A N/A N/A N/A N/A U N/A N/A N/A 75 50 30 24 15 12 10 10 8 12 13 18 27 8

12" x 16.5" 30" x 18" 32.5" x 26.7" 30" x 18" 31 x 42 cm 76 x 46 cm 83 x 68 cm 76 x 46 cm D N/A N/A 31 22 13 8 6 2 N/A N/A N/A N/A N/A N/A N/A N/A N/A U 59 35 18 18 8 6 4 2 N/A N/A N/A N/A N/A 3 4 6 N/A D U D U D N/A N/A N/A 60 40 24 15 12 6 N/A N/A N/A N/A N/A N/A N/A N/A U 187 93 93 39 30 24 12 8 6 N/A N/A N/A 7 9 9 16 4

N/A 187 300 264 N/A 93 248 143 N/A 91 161 143 60 39 99 72 40 30 64 42 24 24 42 33 15 14 23 17 12 8 14 § 12 § 6 6 9§ 8§ 6 6 9 8 N/A 6 ^ N/A 6 N/A N/A 8 6 N/A N/A N/A N/A N/A 7 9 9 16 4 N/A N/A N/A N/A N/A 10 10 16 24 6

(*) Innova 42/42R, 4200/4230, Innova 2100 and Excella E5 only. (**) 4900 only. () Innova 4900 accepts up to 4 shaker modules. When using 2 L or 2.8 L flasks, the maximum number of shaker modules is 3. With 4, 5 or 6 L flasks, the maximum shaker modules is 2. The maximum capacity of flasks using a 125 mL Dedicated Platform is decreased to 48 when using a Slide-Out Platform. () Not for use with Innova 4340, Innova 4400/4430. () Maximum flask size when using Photosynthetic Light Bank is 4 Liters. (^) Using Sticky Tape - less capacity with clamps. (§) Only half of bottom tier can be used. () For use on top tier only.

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New Brunswick Scientific · a culture of innovation · www.nbsc.com

Accessories: Clamps & Test Tube Racks

SHAKER PLATFORMS & CLAMPS

Shakers are sold separately from platforms and accessories to provide the flexibility to select the platform(s) best suited to your application. Universal Platforms accommodate a mix of different size glassware on a single platform. Flask clamps, test tube and microplate racks sold separately. Sticky Tape and Sticky Pad provide another alternative for securing vessels to a platform without need for a clamp. (See below.) If using a single flask size only, our Dedicated Platforms include clamps already installed, and maximize platform capacity. Utility Trays provide a non-slip surface for low-speed applications. Utility Carriers secure vessels between adjustable, cushioned rods.

Universal Platform

Universal Platform shown with clamps, test tube racks and sticky tape

Sticky Pad Platform

Dedicated Platform

Utility Tray

Utility Carrier

Flask Clamps & Sticky Tape/Pad

Catalog No. Description

Flask clamps are of a single piece stainless-steel construction (not spot welded or riveted) for added strength and safety. Clamps 2L and above come with an extra girdle for added security when agitating flasks at high speeds. Includes mounting screws. Catalog No. ACSB-500S ACSB-1000S TTR-210 TTR-215 M1289-0700 TTR-221 M1250-9600ø M1250-9504

3

Description 500 mL Media Bottle Clamp with spring retainer 1 L Media Bottle Clamp with spring retainer Angled Tube Rack Holder for user-supplied test tube racks Spacer Bar for TTR-210 Microplate rack, stack 3 deep well or 9 standard plates Microplate rack, for single layer of 5 plates Sticky Tape (16.4' x 1.5") Sticky Pad Adapter Kit Spare Flat Phillips Head Screws, 10­24 x 5/16 , Pack of 25, for aluminum & phenolic platforms Spare Flat Phillips Head Screws, 10­24 x 5/8 , Pack of 25 , for model 2350 (plywood) platform only

ACE-10S 10 mL Erlenmeyer Clamp M1190-9004 25 mL Erlenmeyer Clamp M1190-9000 50 mL Erlenmeyer Clamp with spring retainer M1190-9001 125 mL Erlenmeyer Clamp with spring retainer M1190-9002 250 mL Erlenmeyer Clamp with spring retainer M1190-9003 500 mL Erlenmeyer Clamp with spring retainer ACE-1000S 1 L Erlenmeyer Clamp with spring retainer ACE-2000S 2 L Erlenmeyer Clamp with spring retainer M1190-9005*** 2 L Lauber Clamp with spring retainer ACFE-2800S 2.8 L Fernbach Clamp with spring retainer ACE-3000S 3 L Erlenmeyer Clamp with spring retainer ACE-4000S 4 L Erlenmeyer Clamp with spring retainer ACE-5000S 5 L Erlenmeyer Clamp with spring retainer ACE-6000S 6 L Erlenmeyer Clamp with spring retainer

() (ø) (**) (***) (3)

M1250-9700** Sticky Pad, 8" x 8" (20 x 20 cm) S2116-3051P S2116-3101P

Flask clamps are stainless steel, come complete with required screws, and are fitted with coiled wire springs for added stability at elevated speeds. Replaces the need for clamps when shaking at speeds up to 250 rpm. Not for use with water bath shakers, or at speeds above 250 rpm. Sticky Pad should be used on an anodized aluminum substrate. Select from M1250-9504, M1250-9903, M1250-9904 or M1282-9913. Fits 2L Thomson flask with our universal platform. Two anodized aluminum sheets each 6" x 18" (15.2 x 45.7 cm). Used with Sticky Pad on aluminum Universal platforms.

Microplate Racks

Test Tube Racks

Large Racks

Part number M1289-0100 M1289-0200 M1289-0300 M1289-0400 M1289-0500 M1289-0600 Diameter 8-11 mm 12-15 mm 15-18 mm 18-21 mm 22-26 mm 26-30 mm Tubes/Rack 80 60 42 30 22 20

Test Tube Racks have an adjustable angle and tube height support. Made from stainlesssteel with inserts to help retain tubes and prevent them from spinning in the rack. Fingertight knobs let you quickly adjust angle, or release the rack from the base plate, for easy transport to the bench.

Medium Racks

Part number M1289-0010 M1289-0020 M1289-0030 M1289-0040 M1289-0050 M1289-0060 Diameter 8-11 mm 12-15 mm 15-18 mm 18-21 mm 22-26 mm 26-30 mm Tubes/Rack 60 44 31 23 16 16 Part number M1289-0001 M1289-0002 M1289-0003 M1289-0004 M1289-0005 M1289-0006

Small Racks

Diameter 8-11 mm 12-15 mm 15-18 mm 18-21 mm 22-26 mm 26-30 mm Tubes/Rack 48 34 24 18 13 12

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5

Innova Incubator Shakers

®

Innova® 44 Stackable Incubator Shakers & 44R with Added Refrigeration - Programmable

Space-saving Innova 44 incubator shakers, and 44R with added refrigeration, stack up to three high and have front door that glides up to further minimize space requirements. Slide-out platform: 30" x 18" (76 x 46 cm). (Platform sold separately.) Temperature range**: Innova 44: 5°C above ambient to 80°C; Innova 44R: 20°C below ambient to 80°C (minimum 4°C). Speed (rpm) : 25 - 400 with 1" orbit; 25 - 300 with 2" orbit or when stacking 2 units; 25 - 250 when stacking three units. Programmable controller automatically changes temperature, speed, optional photosynthetic and UV germicidal lights at timed intervals. Overall dimensions: 53" W x 34.4" D x 26" H (135 x 87.3 x 66 cm). Height: add 26"(66 cm) for each added unit; door open adds 21" (53 cm). Net weight: Innova 44: 523 lbs. (238 kg); Innova 44R: 566 lbs. (257 kg).

Model Catalog No. 1" (2.54 cm) Orbit M1282-0000 M1282-0001 M1282-0002 M1282-0003 M1282-0004 M1282-0005 M1282-0006 M1282-0007 Catalog No. 2" (5.08 cm) Orbit M1282-0010 M1282-0011 M1282-0012 M1282-0013 M1282-0014 M1282-0015 M1282-0016 M1282-0017 Electrical Service 120V, 60Hz 100V, 60Hz 230V, 50Hz 100V, 50Hz 120V, 60Hz 100V, 60Hz 230V, 50Hz 100V, 50Hz

Three shakers shown. Slide-out platforms provide easy access to all flasks.

44 Incubated 44R Incubated/ Refrigerated

() Shaker does not include platform which must be ordered separately. (**) Maximum temp. is 60°C when using optional RH humidity probe and 55°C above ambient without RH probe. () Less with baffled flasks. () Refrigerated 50 Hz units temperature range: ambient -17°C to 80°C.

Innova 44/44R Interchangeable Platforms, Slide-Out

30" x 18" (76 x 46 cm) Aluminum unless otherwise stated below. (Not interchangeable with other 30" x 18" platforms)

Catalog No. M1282-9904 * M1282-9915 * M1282-9905 M1282-9906 M1282-9907 M1282-9908 M1282-9925 M1282-9909 M1282-9926 M1282-9910 M1282-9927 M1282-9911 M1282-9928 M1282-9913 ^ Description Universal Platform, (can be used with New Sticky Tape) Universal Platform, 3/8" Aluminum Plate 125 mL Erlenmeyer Flask Dedicated Platform 250 mL Erlenmeyer Flask Dedicated Platform 500 mL Erlenmeyer Flask Dedicated Platform 1 L Erlenmeyer Flask Dedicated Platform 1 L Dedicated Platform, 3/8" Aluminum Plate 2 L Erlenmeyer Flask Dedicated Platform 2 L Dedicated Platform, 3/8" Aluminum Plate 2.8 L Fernbach Flask Dedicated Platform 2.8 L Dedicated Platform, 3/8" Aluminum Plate 4 L Erlenmeyer Flask Dedicated Platform 4 L Dedicated Platform, 3/8" Aluminum Plate Sticky Pad Platform Clamps 1 Shaker -- -- 60 40 24 15 15 12 12 6 6 6 6 -- Clamps 2 Shakers -- -- 120 80 48 30 30 24 24 12 12 12 12 -- Clamps 3 Shakers -- -- 180 120 72 45 45 36 36 18 18 18 18 --

(*) Flask clamps, test tubes and Sticky Tape for Universal Platforms must be ordered separately. See page 5. (^) Sticky Pad sold separately. See page 5. () Recommended for use with a full load of flasks, 1 liter or larger, containing more than 20% volume and/or baffles.

Innova 44/44R Optional Accessories

(Not interchangeable with Innova 4400 Series Accessories)

Catalog No. Description Base: Raises shaker height for easier access to chamber & controls. M1282-0600 Short Base to raise shakers by 4"(10.2 cm). For use with up to three stacked units M1282-0800 Medium Base to raise shakers by 12"(30.5 cm). For use with up to two stacked units M1282-0700 Tall Base to raise shakers by 16" (40.6 cm). For use with a single unit Stacking Kit: Bolts shakers together for stability when stacking two or more units. One needed for two shakers. Two needed for three shakers. M1282-0500 Stacking Kit to join two Innova 44/44R Shakers M1282-0501 Stacking Kit to join Innova 44/44R with Innova 4400. (44 shakers must be installed below 4400). Other Options: M1282-9922 Photosynthetic Light Bank for Model 44R only, factory installed M1282-0291 12-Port Gassing Manifold for direct gassing into flasks, factory installed M1282-5011 Humidity Monitor, factory installed M1282-9921 UV Germicidal Lamp for Model 44R only, factory installed M1282-8038 Remote Alarm, factory installed M1282-6000 Spare Parts Kit (refer to page 2 for details)

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Innova® 43 Console Incubator Shakers & 43R with Added Refrigeration - Programmable

Large-capacity Innova 43 Incubator Shakers and Model 43R with added refrigeration accommodate numerous culture flasks up to 6 liters. Platform: 30" x 18" (76 x 46 cm) sold separately.) Temperature range: Model 43: 5°C above ambient to 80°C; Model 43R: from 20°C below ambient to 80°C (minimum setpoint 4°C). Speed: 25 - 500 in models with 1" orbit; 25 - 300 rpm with 2" orbit. Programming capability automatically changes temperature, speed, optional photosynthetic and UV germicidal lights at timed intervals. Overall dimensions: 45.5" W x 32" D x 40.6" H (115.6 x 81.3 x 103.1 cm). With door open, height is 63 1/2" (161 cm). Net weight: Innova 43 - 420 lbs. (191 kg); Innova 43R - 475 lbs. (204 kg).

Model Catalog No. 1" (2.54 cm) Orbit M1320-0000 M1320-0001 M1320-0002 M1320-0003 M1320-0004 M1320-0005 M1320-0006^ M1320-0007^ Catalog No. 2" (5.1 cm) Orbit M1320-0010 M1320-0011 M1320-0012 M1320-0013 M1320-0014 M1320-0015 M1320-0016 M1320-0017 Electrical Service 120V, 60Hz 100V, 60Hz 230V, 50Hz 100V, 50Hz 120V, 60Hz 100V, 60Hz 230V, 50Hz 100V, 50Hz

43 Incubated 43R Incubated/ Refrigerated

() Shaker does not include 30" x 18" platforms which must be ordered separately. See below. () Baffled flasks will significantly reduce maximum speed. () With Photosynthetic lights, maximum flask size is 4 liters. (^) Temperature range is Ambient -17°C to 80°C for refrigerated 50 Hz units. .

Innova 43 & 43R Interchangeable Platforms

30" x 18" (76 x 46 cm) Aluminum

Catalog No. M1250-9920* M1243-2500 AG-00 M1191-9908 M1191-9909 M1191-9910 M1191-9911 AG-1 AG-2 AG-28 AG-4 AG-6 M1250-9904 Description Universal Platform Microplate Platform. Holds 105 standard or 21 deep well plates Utility Tray with non-skid surface 50 mL Erlenmeyer Flask Dedicated Platform 125 mL Erlenmeyer Flask Dedicated Platform 250 mL Erlenmeyer Flask Dedicated Platform 500 mL Erlenmeyer Flask Dedicated Platform 1 L Erlenmeyer Flask Dedicated Platform 2 L Erlenmeyer Flask Dedicated Platform 2.8 L Fernbach Flask Dedicated Platform 4 L Erlenmeyer Flask Dedicated Platform 6 L Erlenmeyer Flask Dedicated Platform Platform for Sticky Pad Clamps -- -- -- 108 60 40 24 15 12 6 6 4 --

(*) Flask clamps and test tube racks for Universal Platform must be ordered separately. See page 5. (1) AG-6 Platform can not be used with photosynthetic light bank. (2) Sticky Pad ordered separately. See page 5.

Innova 43 & 43R Accessories

Catalog No. M1320-0500 M1320-0300 M1320-0400 M1320-0600 M1320-8029 M1320-0700 M1282-6000 M1320-6000 Description 12-Port Gas Manifold for direct gassing into flasks, factory installed Photosynthetic Light Bank for Model 43R only, factory installed UV Germicidal Lamp for Model 43R only, factory installed Humidity Monitor, factory installed Remote Alarm, factory installed Utility Basket for incubating/refrigerating stationary glassware Spare Parts Kit includes drive belt and fuses Spare Parts Kit includes drive belt, fuses, chamber bulbs and hinge assemblies

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7

Innova Incubator Shakers

®

Innova® 42 Stackable, On-or-Under-Bench Incubator Shakers & 42R with Added Refrigeration - Programmable

NEW

Our most versatile incubator shakers, programmable 42 & 42R provide "set it and forget it" convenience. Use on or under the bench, or double stacked for space savings §. Programming capability automatically changes temperature and speed as well as optional Photosynthetic Lighting and UV Germicidal lights at timed intervals. Optional culture drawer and humidity probe are available for both the 42 and 42R. Optional photosynthetic light bank and UV decontamination available for the 42R only. Temperature range: Innova 42: 5°C above ambient to 80°C; Innova 42R: 20°C below ambient to 80°C. Speed: 25 - 400 rpm. Platform: 18" x 18" (46 x 46 cm) accommodates flasks up to 4 liters. (Platform sold separately.) Overall dimensions: 25" W x 29.7" D x 32.2" H (62 x 75.4 x 82 cm). Height stacked: 64.50" (164 cm). Depth with door open: 51.5" (130.8 cm). Net weight: Innova 42: 217 lbs. (98.5 kg); Innova 42R: 267 lbs. (121 kg).

Model Catalog No. 3/4" (1.92 cm) Orbit M1335-0000 M1335-0002 M1335-0001 M1335-0003 M1335-0004 M1335-0006 M1335-0005 M1335-0007 Catalog No. 1" (2.54 cm) Orbit M1335-0010 M1335-0012 M1335-0011 M1335-0013 M1335-0014 M1335-0016 M1335-0015 M1335-0017 Electrical Service 120V, 60 Hz 230V, 50 Hz 100V, 60 Hz 100V, 50 Hz 120V, 60 Hz 230V, 50 Hz 100V, 60 Hz 100V, 50 Hz

42 Incubated 42R Incubated/ Refrigerated

Shown stacked two units high. Stationary shelf and internal power receptable are standard.

() Shaker does not include 18" x 18" platforms which must be ordered separately. (§) Stacking kit required when stacking 2 units. See accessories below. () Baffled flasks will significantly reduce maximum speed. When stacked, maximum speed is 400 rpm with 250/500 mL flasks; maximum 300 rpm or lower with larger flasks.

Innova 42 & 42R Interchangeable Platforms - See next page Innova 42 & 42R Optional Accessories

Catalog No. M1335-0300 M1335-0500 M1335-0400 M1335-0501 M1335-0800 M1335-0600 M1335-0080 M1320-8029 M1335-6000 Description Photosynthetic Light Bank for Model 42R only, factory installed Humidity Monitor, factory installed UV Germicidal Light for Model 42R only, factory installed Culture Drawer, prevents plates from drying Stacking Kit, required when stacking 42/42R units (See also M1335-0810 below) 12-Port Gassing Manifold for direct gassing into flasks, factory installed Additional Shelf & Brackets Remote Alarm, factory installed Spare Parts Kit, all voltages

Innova 4200 & 4230 Optional Accessories

Catalog No. M1233-9996 M1233-6002 M1335-0810 Description Additional Shelf & Brackets Spare Parts Kit - 120V, 50/60 Hz Stacking Kit, to stack Innova 42/42R units on Innova 4200/4230 shakers

() Refer to page 2 for details of Spare Parts Kits.

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Innova® 40 Benchtop Incubator Shakers & 40R with Added Refrigeration - Programmable

Innova 40 incubator shakers provide high-temperature capability for culturing a wide variety of organisms in flasks up to 3 liters. Refrigeration module is built into the Innova 40R, or can be easily added to Model 40, at any time. Temperature range: Innova 40: 5°C above ambient to 80°C. Innova 40R: 15°C below ambient to 80°C. Speed: 25 - 500 with choice of 3/4" or 1" orbit (1.9 or 2.54 cm). Programming capability automatically changes temperature and speed at timed intervals. Platform: 18" x 18" (46 x 46 cm), sold separately. Overall dimensions: 22" W x 30" D x 24" H (55.88 x 76.2 x 61 cm). With lid open, height is 40" (102 cm). Net weight: Innova 40:133 lbs. (60.3 kg); Innova 40R: 175 lbs. (79.4 kg).

Model Catalog No. 3/4" (1.92 cm) Orbit M1299-0080 M1299-0081 M1299-0082 M1299-0083 M1299-0084 M1299-0085 M1299-0086 M1299-0087 Catalog No. 1" (2.54 cm) Orbit M1299-0090 M1299-0091 M1299-0092 M1299-0093 M1299-0094 M1299-0095 M1299-0096 M1299-0097 Electrical Service 120V, 60Hz 100V, 60Hz 230V, 50Hz 100V, 50Hz 120V, 60Hz 100V, 60Hz 230V, 50Hz 100V, 50Hz

40 Incubated 40R Incubated/ Refrigerated

() Shaker does not include 18" x 18" platforms which must be ordered separately. () Baffled flasks will significantly reduce maximum speed.

Innova 40 & 40R / 4000 & 4080 Options

Catalog No. M1299-0061 M1320-8029 M1299-6000 M1192-6002 Description Refrigeration Module to upgrade Innova 40 to Innova 40R Remote Alarm, factory installed only Spare Parts Kit - Innova 40 & 40R, (120V, 50/60 Hz) Spare Parts Kit - Innova 4000 & 4080, (120V, 50/60 Hz)

-- --

() Refer to page 2 for details of Spare Parts Kits.

Innova 40 & 40R / 42 & 42R / 4000 & 4080 / 4200 & 4230 Interchangeable Platforms

18" x 18" (46 x 46 cm) Phenolic Resin unless otherwise stated

Catalog No. M1250-9902* M1250-9906 M1194-9909 M1194-9923 M1194-9910 M1194-9903 M1194-9904 M1194-9905 M1194-9906 M1194-9907 M1194-9908 M1233-9932 M1233-9930 M1250-9903 2 M1192-99011 Description Universal Platform, Aluminum Drip Pan for Universal Platform M1250-9902 Utility Carrier holds glassware between rubber-cushioned cross bars Rod Kit for Utility Carrier (one additional rod) Utility Tray with non-skid surface 50 mL Erlenmeyer Flask Dedicated Platform 125 mL Erlenmeyer Flask Dedicated Platform 250 mL Erlenmeyer Flask Dedicated Platform 500 mL Erlenmeyer Flask Dedicated Platform 1 L Erlenmeyer Flask Dedicated Platform 2 L Erlenmeyer Flask Dedicated Platform 2.8 L Fernbach Flask Dedicated Platform 4 L Erlenmeyer Flask Dedicated Platform Sticky Pad Platform Quick-Change Platform Kit Clamps -- -- -- -- -- 64 34 25 16 9 5 4 4 -- --

(*) Flask clamps for Universal Platform must be ordered separately. See page 5. (2) Sticky Pad sold separately, see page 5. (1) Maximum speed 400 rpm. () Available for models 42/42R and 4200/4230 only.

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9

Innova Water Bath Shakers

®

Innova® 3100 High-Temperature Water Bath Shakers

Large-capacity Gyrotory shaker with microprocessor control of speed and temperature. One-piece stainless steel bath. Adjustable water-level control automatically replenishes evaporated water. Has drain, water-in and overflow lines. Speed: 25-400 rpm (less for baffled flasks). Orbit: 1/2" (1.3 cm). Temperature range: 5°C above ambient to 100°C.§ Platform: 12" x 16.5" (30.5 x 42 cm), sold separately. Overall dimensions: 17" W x 27.5" D x 14.5" H (43 x 70 x 37 cm). Net weight: 115 lbs. (63 kg).

Model Catalog No. M1231-0000 M1231-0001 M1231-0002 M1231-0003 Electrical Service 120V, 50/60Hz 100V, 50/60Hz 220/230V, 50/60Hz 240V, 50/60Hz

3100 Water Bath

For flasks and gel trays

() Shaker does not include 12" x 16.5" platforms which must be ordered separately. See below. (§) Elevated temperatures attained with aid of a gable cover using bath liquid with a high boiling point to minimize evaporation. For lower temperatures an optional cooling assembly is available. See below.

Innova 3100 Interchangeable Platforms & Accessories

12" x 16.5" (30.5 x 42 cm) Stainless Steel

Catalog No. M1231-9930* M1231-9933 M1231-9934 M1231-9935 M1231-9936 M1231-9937 M1231-9938 M1231-9939 Description Universal Platform 50 mL Erlenmeyer Flask Dedicated Platform 125 mL Erlenmeyer Flask Dedicated Platform 250 mL Erlenmeyer Flask Dedicated Platform 500 mL Erlenmeyer Flask Dedicated Platform 1 L Erlenmeyer Flask Dedicated Platform 2 L Erlenmeyer Flask Dedicated Platform Sub-Platform for mounting half-size platforms containing flasks and test-tube racks. Sub-platform holds 2 half-size flask platforms or 4 racks. Clamps -- 31 22 13 8 6 2 --

Half-Size Platforms (Half platforms require Sub-Platform listed above)

Catalog No. AG7-50 AG7-125 AG7-250 Half Platforms Description Per Bath 50 mL Erlenmeyer Flask Half Platform 2 125 mL Erlenmeyer Flask Half Platform 2 250 mL Erlenmeyer Flask Half Platform 2 Clamps Per Platform 13 8 5

Half-Size Platforms with accessory clamps

Test Tube Racks

Catalog No. AG7-TT13 AG7-TT16 AG7-TT20 AG7-TT25 Description Rack for 13 mm Tubes Rack for 16 mm Tubes Rack for 20 mm Tubes Rack for 25 mm Tubes Racks Per Bath 4 4 4 4 Tubes Per Rack 60 36 29 18

Additional Accessories

Catalog No. M1231-2010 M1231-2000 Stainless Steel Gable Cover M1231-9920 M1195-1020 M1231-0730 M1020-1220 M1231-6000 Description Clamps Gable Cover, Plexiglas, minimizes heat loss and heat-up time. Not recommended for temperatures above 60°C. Gable Cover, Stainless Steel, for high-temperature applications up to 100°C. Reduces heat-up time. Minimizes evaporation. Cooling Assembly, for below ambient temperature applications. Must be factory installed. Space-Saving Dolly, (Under lab bench operation) 18" W x 28.25" long (46 x 72 cm). Overall height, of shaker on dolly: 20"(51 cm) Spare Set of Arms Portable Cart, 221/2"W x 32.25"L, (57 x 87 cm). Overall height, shaker on cart: 42.5" (108 cm) Spare Parts Kit

Cooling Assembly

(*) Flask clamps for Universal Platform must be ordered separately. For platform capacities and clamp information see page 5. () Refer to page 2 for details of Spare Parts Kit.

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Innova Open Air Shakers

®

Innova® 2000, 2100 & 2300 Series Benchtop Open Air Shakers

Low-profile shakers with Triple-Eccentric Drive for uniform motion and microprocessor controls for accurate regulation of shaking speed, 25 ­ 500 rpm (less with baffled flasks). Includes audible and visual alarms, timer (0 ­ 99.9 hrs) with continuous operation or automatic shut-off at the end of Model No. Innova 2000 Innova 2050 Innova 2100 Innova 2150 Innova 2300 Innova 2350 Platform Size Inches cm 13 x 11 16 x 12 18 x 18 24 x 18 30 x 18 36 x 24 33 x 28 40.6 x 30.5 45.7 x 45.7 61 x 45.7 76.2 x 45.7 91.4 x 61 Capacity/ Orbit Flask size 6 x 500 mL 3x2L 5x2L 8x2L 4x6L 6x6L 3/4" 3/4" 3/4" 3/4" 1"or 2" 1"or 2" the study. Models 2000, 2100 and 2300 provide for small, intermediate and large-capacity shaking, respectively. Models 2050, 2150 and 2350 come with additional stabilizing feet and/or counterweight to balance larger loads on oversized platforms. Platforms sold separately, see pages 12 ­ 13. Overall Dimensions Inches (W x D x H) cm (W x D x H) 14 x 14.5 x 5.75 17 x 14.5 x 5.75 19 x 21.9 x 6.4 24 x 23.3 x 6.4 30 x 22.3 x 6.4 36 x 24 x 6.4 35.5 x 37 x 14.6 43 x 37 x 14.6 48 x 55.5 x 16.3 60.1 x 59.1 x 16.3 76.2 x 56.6 x 16.3 91.4 x 61 x 16.3 Net Weight lbs. kg. 33 35 75 80 105 115 18.6 19.1 54.4 57 66 68

() Indicates number of largest flask size with dedicated platform. Universal platform capacity may be less. Flask clamps for Universal Platform must be ordered separately. See page 5.

Model

Catalog No. M1190-0000 M1190-0001 M1190-0002 M1190-0003 M1190-0010 M1190-0011 M1190-0012 M1190-0013 M1194-0000 M1194-0001 M1194-0002 M1194-0003 M1194-0010 M1194-0011 M1194-0012 M1194-0013 M1191-0000 M1191-0001 M1191-0002 M1191-0003 M1191-0020 M1191-0021 M1191-0022 M1191-0023 M1191-0010 M1191-0011 M1191-0012 M1191-0013 M1191-0030 M1191-0031 M1191-0032 M1191-0033

Electrical Service 120V, 50/60Hz 100V, 50/60Hz 220/230V, 50/60Hz 240V, 50/60Hz 120V, 50/60Hz 100V, 50/60Hz 220/,230V, 50/60Hz 240V, 50/60Hz 120V, 50/60Hz 100V, 50/60Hz 220/230V, 50/60Hz 240V, 50/60Hz 120V, 50/60Hz 100V, 50/60Hz 220/230V, 50/60Hz 240V, 50/60Hz 120V, 50/60Hz 100V, 50/60Hz 220/230V, 50/60Hz 240V, 50/60Hz 120V, 50/60Hz 100V, 50/60Hz 220/230V, 50/60Hz 240V, 50/60Hz 120V, 50/60Hz 100V, 50/60Hz 220/230V, 50/60Hz 240V, 50/60Hz 120V, 50/60Hz 100V, 50/60Hz 220/230V, 50/60Hz 240V, 50/60Hz

2000

2050

2100

2150 2300

1" Stroke (2.54 cm)

2300

2" Stroke (5.08 cm)

For multi-shaker racking system, see page 14.

2350

1" Stroke (2.54 cm)

2350

2" Stroke (5.08 cm)

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11

Innova Open Air Shakers

®

Innova® 2000 Interchangeable Platforms & Accessories

13" x 11" (33 x 28 cm) Stainless Steel

Catalog No. M1001-0240* AG2-UT AG2-00 M1001-9920 AG2-10 M1190-9919 M1190-9915 M1190-9916 M1190-9917 M1190-9918 M1190-0300 M1190-9910§ M1190-9909 M1190-6100 Description Universal Platform Utility Carrier holds a variety of glassware between rubber-cushioned cross bars Utility Tray with non-skid rubber surface Rod Kit for Utility Carrier (one additional rod) 10 mL Erlenmeyer Flask Dedicated Platform 25 mL Erlenmeyer Flask Dedicated Platform 50 mL Erlenmeyer Flask Dedicated Platform 125 mL Erlenmeyer Flask Dedicated Platform 250 mL Erlenmeyer Flask Dedicated Platform 500 mL Erlenmeyer Flask Dedicated Platform Sticky Pad Platform Capacity Upgrade Kit, converts Model 2000 to a Model 2050 Temperature & Speed Monitoring Kit for recording on an external chart recorder Spare Parts Kit, Innova 2000/2050 (120V, 50/60Hz) Clamps -- -- -- -- 60 32 20 12 8 6 -- -- -- --

Innova 2050 Interchangeable Platforms

16" x 12" (40.6 x 30.5 cm) Stainless Steel

Catalog No. M1190-9900* M1190-9907 M1190-9920 M1190-9908 M1190-9901 M1190-9902 M1190-9903 M1190-9904 M1190-9905 M1190-9906 M1190-9911 M1190-6100 Description Universal Platform Utility Carrier holds a variety of glassware between rubber-cushioned cross bars Rod Kit for Utility Carrier (one additional rod) Utility Tray with non-skid rubber surface 10 mL Erlenmeyer Flask Dedicated Platform 25 mL Erlenmeyer Flask Dedicated Platform 50 mL Erlenmeyer Flask Dedicated Platform 125 mL Erlenmeyer Flask Dedicated Platform 250 mL Erlenmeyer Flask Dedicated Platform 500 mL Erlenmeyer Flask Dedicated Platform 1 L Erlenmeyer Flask Dedicated Platform Spare Parts Kit, Innova 2000/2050 (120V, 50/60Hz) Clamps -- -- -- -- 86 48 35 20 12 8 6 --

Innova 2100 Interchangeable Platforms and Accessories

18" x 18" (45.7 x 45.7 cm) Phenolic Resin - Unless otherwise stated

Catalog No. M1250-9902* M1250-9906 M1194-9909 M1194-9923 M1194-9910 M1194-9903 M1194-9904 M1194-9905 M1194-9906 M1194-9907 M1194-9908 M1233-9932 M1250-9903** M1194-9926§ M1192-9901 M1194-9924 M1194-6001 Description Universal Platform, Aluminum Drip Pan to catch accidental spills for use with Universal Platform M1250-9902 Utility Carrier holds a variety of glassware between rubber-cushioned cross bars Rod Kit for Utility Carrier (one additional rod) Utility Tray with non-skid rubber surface 50 mL Erlenmeyer Flask Dedicated Platform 125 mL Erlenmeyer Flask Dedicated Platform 250 mL Erlenmeyer Flask Dedicated Platform 500 mL Erlenmeyer Flask Dedicated Platform 1 L Erlenmeyer Flask Dedicated Platform 2 L Erlenmeyer Flask Dedicated Platform 2.8 L Fernbach Flask Dedicated Platform Sticky Pad Platform Capacity Upgrade Kit to convert a Model 2100 to a Model 2150 Quick-Change Platform Kit, Innova 2100/4000/4230 Temperature & Speed Monitoring Kit for recording on an external chart recorder. For Models 2100, 2150, 2300 & 2350 Spare Parts Kit, Innova 2100/2150 (120V, 50/60Hz) Clamps -- -- -- -- -- 64 34 25 16 9 5 4 -- -- -- -- --

(*) Flask clamps for Universal Platform must be ordered separately. See page 5. (**) Sticky Pad sold separately, see page 5. (§) Includes counterweight and hardware to balance larger workloads. () Enables platforms to be snapped in place without tools; platforms must be ordered separately; maximum speed is 400 rpm. () Refer to page 2 for details of Spare Parts Kits.

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Innova® 2150 Interchangeable Platforms & Accessories

24" x 18" (61 x 45.7 cm) Phenolic Resin

Catalog No. M1194-9912* M1194-9921 M1194-9925 M1194-9922 M1194-9915 M1194-9916 M1194-9917 M1194-9918 M1194-9919 M1194-9920 M1194-9924 M1194-9927 M1194-6001 Description Clamps Universal Platform -- Utility Carrier holds a variety of glassware between rubber-cushioned cross bars -- Rod Kit for Utility Carrier (one additional rod) -- Utility Tray with non-skid rubber surface -- 50 mL Erlenmeyer Flask Dedicated Platform 80 125 mL Erlenmeyer Flask Dedicated Platform 48 250 mL Erlenmeyer Flask Dedicated Platform 35 500 mL Erlenmeyer Flask Dedicated Platform 20 1 L Erlenmeyer Flask Dedicated Platform 12 2 L Erlenmeyer Flask Dedicated Platform 8 Temperature and Speed Monitoring Kit for recording on an external chart recorder. For Models 2100, 2150, 2300 & 2350 Shakers -- Quick-Change Platform Kit -- Spare Parts Kit, Innova 2100/2150 (120V, 50/60Hz) --

Innova 2300 Interchangeable Platforms & Accessories

30" x 18" (76.2 x 45.7 cm) Aluminum

Catalog No. M1250-9920* M1250-9921 AG-00 M1191-9908 M1191-9909 M1191-9910 M1191-9911 AG-1 AG-2 AG-4 AG-6 AG-28 M1250-9904** M1191-9905§ M1191-9904 M1194-9924 M1191-6102 M1243-2500 Description Clamps Universal Platform -- Drip Pan to catch accidental spills for use with Universal Platform, M1250-9920 -- Utility Tray with non-skid rubber surface -- 50 mL Erlenmeyer Flask Dedicated Platform 108 125 mL Erlenmeyer Flask Dedicated Platform 60 250 mL Erlenmeyer Flask Dedicated Platform 40 500 mL Erlenmeyer Flask Dedicated Platform 24 1 L Erlenmeyer Flask Dedicated Platform 15 2 L Erlenmeyer Flask Dedicated Platform 12 4 L Erlenmeyer Flask Dedicated Platform 6 6 L Erlenmeyer Flask Dedicated Platform 4 2.8 L Fernbach Flask Dedicated Platform 6 Sticky Pad Platform -- Capacity Upgrade Kit to convert a Model 2300 to a Model 2350 -- Quick-Change Platform Kit, Innova 2300 -- Temperature and Speed Monitoring Kit for recording on an external chart recorder. For Models 2100, 2150, 2300 & 2350 Shakers -- Spare Parts Kit, Innova 2300/2350, (120V, 50/60Hz) -- Microplate platform holds 105 standard or 21 deep well plates --

Innova 2350 Interchangeable Platforms & Accessories

36" x 24" (91.4 x 60.9 cm) Marine Plywood unless otherwise stated

Catalog No. PTL-386 PTL-393 AG-21-00 M1191-9912 M1191-9913 M1191-9914 M1194-9924 Description Universal Platform Universal Platform, aluminum Utility Tray with non-skid rubber surface 125 mL Erlenmeyer Flask Dedicated Platform 250 mL Erlenmeyer Flask Dedicated Platform 500 mL Erlenmeyer Flask Dedicated Platform Temperature and Speed Monitoring Kit for recording on external recorder. For Models, 2100, 2150, 2300 & 2350 Spare Parts Kit, Innova 2300/2350, (120V, 50/60Hz) Clamps -- -- -- 96 70 40

M1191-6102

-- --

(*) Flask clamps for Universal Platform must be ordered separately. For platform capacities and clamp information see page 5. (**) Sticky Pad sold separately. See page 5. (§) Includes counterweight, hardware and outrigger feet to balance larger workloads. () Enables platforms to be snapped in place without tools; platforms must be ordered separately. Maximum speed with 1" orbit shaker is 400 rpm. () Refer to page 2 for details of Spare Parts Kits.

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13

Innova Multi-Tier Shakers

®

Innova® 5000 Multi-Tier Shaker -- An Industrial Workhorse

Culture hundreds of samples on this industrial workhorse. Speed: 25 to 350 rpm (less for baffled flasks). Orbit: 2" (5.08 cm). Counterbalanced shaker imparts uniform agitation to all samples without being bolted to the floor. Cantilevered design provides unobstructed access to all glassware. Six removable platforms: 32.5 x 26.75" (82.5 x 68 cm) accommodate flasks 10 mL to 6 L. (Platforms sold separately.) Digital display indicates speed, setpoint, running time and alarm conditions. Overall dimensions: 62" W x 38" D x 53" H (157.5 x 96.5 x 135 cm). Net weight (with platforms): 1,554 lbs. (706 kg).

Model Catalog No. M1196-0000 M1196-0001 M1196-0002 M1196-0003 Electrical Service 120V, 50/60Hz 100V, 50/60Hz 220V, 50/60H 240V, 50/60Hz

Innova 5000

() Shaker does not include 32.5 x 26.75" Platforms which must be ordered separately. See below.

Simultaneously agitate hundreds of samples on up to six accessory platforms

Innova 5000 Interchangeable Platforms & Accessories

32.5" x 26.75" (82.5 x 68 cm) Phenolic Resin. Capacity: 6 Platforms per Shaker

Flask Catalog No. M1196-9447** M1196-9900 M1196-9901 M1196-9902 M1196-9903 M1196-9904 M1196-9905 M1196-9906 M1196-9907 M1196-6000 No. of Flasks Description Per Platform Universal Platform -- 125 mL Erlenmeyer Flask Dedicated Platform 99 250 mL Erlenmeyer Flask Dedicated Platform 64 500 mL Erlenmeyer Flask Dedicated Platform 42 1 L Erlenmeyer Flask Dedicated Platform 23 2 L Erlenmeyer Flask Dedicated Platform 14 2.8 L Fernbach Flask Dedicated Platform 9 4 L Erlenmeyer Flask Dedicated Platform 8 6 L Erlenmeyer Flask Dedicated Platform 8 Spare Parts Kit (120V, 50/60Hz) No. of Flasks Per Machine -- 594 384 252 138 70 54 16 16

(**) Flask clamps for Universal Platform must be ordered separately. For platform capacities and clamp information see page 5. () Can only use front half of platform on bottom tier. () For use on top tier only. Maximum, 16 flasks on 2 top trays. () Please refer to page 2 for details of Spare Parts Kits.

Innova 5050/5051 Racking Systems for Innova 2300 Shakers

Model 5050 consists of a sturdy, stainless-steel rack to hold up to four Innova 2300 Shakers. Innova 5051 rack extension can be added to support an additional four shakers, bringing the total number to eight. Each unit includes an electrical strip for powering the shakers. Shakers must be ordered separately. See page 11 & 13 for Innova 2300 shaker and platform ordering information. Maximum speed: 400 rpm for 1" (2.54 cm) orbit, and 300 rpm for 2" (5.08 cm) orbit shakers. See chart below for rack capacities. Overall dimensions 5050 or 5051: 39" W x 27" D x 72" H (99.1 x 68.6 x 182.9 cm).

Model Catalog No. M1232-0100 M1232-0101 M1232-0110* M1232-0111* Description 5050 Rack System 5050 Rack System 5051 Rack Extension 5051 Rack Extension Electrical Service 100V/120V, 50/60Hz 230V/240V, 50/60Hz 100V/120V, 50/60Hz 230V/240V, 50/60Hz

Innova 5050 & 5051

Innova 5050 & 5051 Extension Racks shown. Shakers sold separately.

Max. Shakers Max. Flasks with Max. Flasks with Max. Flasks with Flask Size per Rack Innova 2300 Innova 5050 Rack Innova 5051 Extension 50 mL 4 108 432 864 125 mL 4 60 240 480 250 mL 4 40 160 320 500 mL 4 24 96 192 1L 3 15 45 90 3 12 36 72 2 L ** (*) Must be used with Model 5050 Rack System. (**) Larger flasks can be used by removing one or more adjustable-height shelves.

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I Series Incubator Shakers

I26 Stackable Incubator Shakers & I26R with added Refrigeration

Stackable, incubated I26 and Incubated/Refrigerated Model I26R Shakers feature NBS' triple-eccentric drive for uniform motion and dependable operation. Based on the design concepts of the Innova shakers, the I26 & I26R offer excellent performance at a value price. Platform: 30" x 18" (76.2 x 45.72 cm) slides out for easy access to all flasks. (Platform sold separately). Speed: 25 - 400 with a 1" orbit (2.54 cm). Temperature range: I26: 5°C above ambient to 60°C; I26R: 15°C below ambient to 60°C. Overall dimensions: 50" W x 30" D x 25.25" H (127 x 76.2 x 64.14 cm)*. Add 25.25" (64 cm) for each additional unit. With door open, add 19.5" (49.5 cm) to depth. Net weight: I26: 370 lbs. (168 kg); I26R: 400 lbs. (182 kg).

Model Catalog No. 1" (2.54 cm) Orbit M1324-0000 M1324-0001 M1324-0002 M1324-0003 M1324-0004 M1324-0005 M1324-0006 M1324-0007 Electrical Service 120V, 60Hz 100V, 60Hz 230V, 50Hz 100V, 50Hz 120V, 60Hz 100V, 60Hz 230V, 50Hz 100V, 50Hz

I26 Incubated I26R Incubated/ Refrigerated

Stacks up to 3 shakers. 2 shakers shown on a medium base

() Shaker does not include platform which must be ordered separately. See below. () Less with baffled flasks. (*) When using 1 unit on the floor, height is 27" (68.58 cm) including shaker feet. When using shaker on a base or stacked, no feet are required and each shaker will be 25.25" high (64.14 cm).

I26 & I26R Interchangeable Platforms, Slide-Out

30" x 18" (76.2 x 46 cm) Aluminum

Platforms are not interchangeable with other 30" x 18" platforms Clamps 1 Shaker -- 60 40 24 15 12 6 -- Clamps 2 Shakers -- 120 80 48 30 24 12 -- Clamps 3 Shakers -- 180 120 72 45 36 18 --

Catalog No. M1324-9904* M1324-9905 M1324-9906 M1324-9907 M1324-9908 M1324-9909 M1324-9910 M1324-9911

Application/Size of Glassware Universal Platform 125 mL Erlenmeyer Flask Dedicated Platform 250 mL Erlenmeyer Flask Dedicated Platform 500 mL Erlenmeyer Flask Dedicated Platform 1 L Erlenmeyer Flask Dedicated Platform 2 L Erlenmeyer Flask Dedicated Platform 2.8 L Fernbach Flask Dedicated Platform Sticky Pad Platform

(*) Flask clamps and test tubes for Universal Platforms must be ordered separately. See page 5.

I26 & I26R Optional Accessories

Accessories are not interchangeable with any other shaker

Catalog No. Description Base: Raises shaker height for easier access to chamber & controls. M1324-0600 Short Base to raise shakers by 4"(10.2 cm). For use with up to three stacked units M1324-0800 Medium Base to raise shakers by 13"(33 cm.) For use with up to two stacked units M1324-0700 Tall Base to raise shakers by 17" (43.2 cm). For use with a single unit Stacking Kit: Bolts shakers together for stability when stacking two or more units. One needed for two shakers; Two needed for three shakers. M1324-0500 Stacking Kit to join two I26/I26R Shakers

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15

Excella Shakers

®

Excella® Shakers -- Excellent Performance & Value

These next-generation shakers from New Brunswick Scientific are offered in eight models to suit a wide variety of culturing applications. Replacing our C-Line, these completely re-designed shakers combine sleek looks, microprocessor controls and heavy-duty counterbalanced drives to provide years of continuous, quiet operation. Digital displays are user friendly and easily seen throughout the lab. Excella shakers are available with a large selection of accessories to accommodate a wide range of flasks, test tubes and general labware. Like most NBS shakers, Excella platforms, clamps and accessories are interchangeable with earlier NBS models.

Excella® E24 Benchtop Incubator Shakers & E24R with Added Refrigeration

Microprocessor-controlled incubator shaker with clear lid allows easy viewing of all flasks. Temperature: Model E24: 7°C above ambient to 60°C; Model E24R: 15°C below ambient to 60°C. Speed: 50 - 400 rpm, (less for baffled flasks). Orbit: 3/4" (1.9 cm). Platform: 18" x 18" (46 x 46 cm), for test tubes and flasks up to 2.8 liters. (Platform sold separately.) Overall dimensions: 22" W x 30" D x 24" H (56 x 76.2 x 61 cm). Net weight: E24: 145 lbs. (66 kg); E24R: 180 lbs. (82 kg).

Model Catalog No. 3/4" (2.54 cm) Orbit M1352-0000 M1352-0001 M1352-0002 M1352-0003 M1352-0004 M1352-0005 M1352-0006 M1352-0007 Electrical Service 120V, 60Hz 100V, 60Hz 230V, 50Hz 100V, 50Hz 120V, 60Hz 100V, 60Hz 230V, 50Hz 100V, 50Hz

E24 Incubated E24R Incubated/ Refrigerated

() Shaker does not include 18" x 18" platforms which must be ordered separately. See below.

Excella E24 & E24R Interchangeable Platforms

18" x 18" (46 x 46 cm) Phenolic Resin unless otherwise stated

Catalog No. M1250-9902* M1250-9906 M1194-9909 M1194-9923 M1194-9910 M1194-9903 M1194-9904 M1194-9905 M1194-9906 M1194-9907 M1194-9908 M1233-9932 M1192-9901 M1250-9903 1 M1352-6000 Description Universal Platform, Aluminum Drip Pan for Universal Platform M1250-9902 Utility Carrier holds glassware between rubber-cushioned cross bars Rod Kit for Utility Carrier (one additional rod) Utility Tray with non-skid surface 50 mL Erlenmeyer Flask Dedicated Platform 125 mL Erlenmeyer Flask Dedicated Platform 250 mL Erlenmeyer Flask Dedicated Platform 500 mL Erlenmeyer Flask Dedicated Platform 1 L Erlenmeyer Flask Dedicated Platform 2 L Erlenmeyer Flask Dedicated Platform 2.8 L Fernbach Flask Dedicated Platform Quick-Change Platform Kit Sticky Pad Platform Spare Parts Kit, E24 & E24R Clamps -- -- -- -- -- 64 34 25 16 9 5 4 -- -- --

(*) Flask clamps for Universal Platform must be ordered separately. See page 5. (1) Sticky Pad sold separately, see page 5

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Excella® E25 Console Incubator Shakers & E25R with Added Refrigeration

Large-capacity, floor model incubator shaker. Temperature: Model E25: 7°C above ambient to 60°C; E25R: 15°C below ambient to 60°C (minimum setpoint of 4°C ). Speed: 50 - 400 rpm (less for baffled flasks). Orbit: 1" (2.54 cm). Platform: 30" x 18" (76 x 46 cm) accommodates a variety of flask capacities up to 6 liters. (Platform sold separately.) Overall dimensions: 50" x 30.5" x 41.7" (127 x 77 x 85 cm). With lid open: 65" H (165 cm). Net weight: Model E25: 377 lbs. (171.2 kg); Model E25R: 417 lbs. (189.3 kg).

Model Catalog No. 1" (2.54 cm) Orbit M1353-0000 M1353-0001 M1353-0002 M1353-0003 M1353-0004 M1353-0005 M1353-0006 M1353-0007 Electrical Service 120V, 60Hz 100V, 60Hz 230V, 50Hz 100V, 50Hz 120V, 60Hz 100V, 60Hz 230V, 50Hz 100V, 50Hz

E25 Incubated E25R Incubated/ Refrigerated

() Shaker does not include 30" x 18" platforms which must be ordered separately. See below.

Excella E10 Large-Capacity Benchtop Open Air Shakers

Large-capacity, microprocessor-controlled shaker with digital display. Speed: 50 - 400 rpm (less for baffled flasks). Orbit: 1" (2.54 cm). Platform: 30" x 18" (76 x 46 cm) accommodates flasks up to 6 liters. Overall dimensions: 30" x 27" x 8.5" (76 x 68.3 x 21.6 cm). Net weight: 100 lbs. (45.4 kg).

Model Catalog No. M1351-0000 M1351-0001 Electrical Service 120/100V, 50/60Hz 230V, 50/60Hz

E10 Open Air

() Shaker does not include 30"x 18" platforms which must be ordered separately. See below.

Excella E25, E25R and E10 Interchangeable Platforms

30" x 18" (76 x 46 cm) Aluminum

Catalog No. M1250-9920** M1250-9921 AG-00 M1191-9908 M1191-9909 M1191-9910 M1191-9911 AG-1 AG-2 AG-28 AG-4 AG-6 M1250-9904^ M1243-2500 Description Universal Platform Drip Pan to catch accidental spills, for Universal Platform M1250-9920 Utility Tray with non-skid rubber surface 50 mL Erlenmeyer Flask Dedicated Platform 125 mL Erlenmeyer Flask Dedicated Platform 250 mL Erlenmeyer Flask Dedicated Platform 500 mL Erlenmeyer Flask Dedicated Platform 1 L Erlenmeyer Flask Dedicated Platform 2 L Erlenmeyer Flask Dedicated Platform 2.8 L Fernbach Flask Dedicated Platform 4 L Erlenmeyer Flask Dedicated Platform 6 L Erlenmeyer Flask Dedicated Platform Platform for Sticky Pad Microplate platform holds 105 standard or 21 deep well plates Clamps -- -- -- 108 60 40 24 15 12 6 6 4 -- --

(**) Flask clamps for Universal Platforms must be ordered separately. See page 5. (^) Sticky Pad sold separately, see page 5. () For use with E10 Shakers only.

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17

Excella Open Air Shakers

®

Excella® E5 Mid-Sized Benchtop Open Air Shakers

Microprocessor-controlled benchtop platform shaker for agitating test tubes and flasks up to 2 L. Speed: 50 - 400 rpm (less for baffled flasks), with electronic speed control. Orbit: 3/4" (1.9 cm). Platform: 18" x 18" (45.7 x 45.7 cm), sold separately. Overall dimensions: 20.5" W x 17.90" D x 8.5" H (52 x 45.5 x 21.6 cm). Net weight: 100 lbs. (45.4 kg).

Model Catalog No. M1355-0000 M1355-0001 Electrical Service 120/100V, 50/60Hz 230V, 50/60Hz

E5

Open Air

() Shaker does not include 18"x 18" platforms which must be ordered separately. See below.

Excella E5 Interchangeable Platforms

18" x 18" (46 x 46 cm) Phenolic Resin unless otherwise stated

Catalog No. M1250-9902 M1250-9906 M1194-9909 M1194-9923 M1194-9910 M1194-9903 M1194-9904 M1194-9905 M1194-9906 M1194-9907 M1194-9908 M1250-9903** Description Universal Platform, Aluminum Drip Pan to catch accidental spills for use with Universal Platform M1250-9902 Utility Carrier holds a variety of glassware between rubber-cushioned cross bars Rod Kit for Utility Carrier (one additional rod) Utility Tray with non-skid rubber surface 50 mL Erlenmeyer Flask Dedicated Platform 125 mL Erlenmeyer Flask Dedicated Platform 250 mL Erlenmeyer Flask Dedicated Platform 500 mL Erlenmeyer Flask Dedicated Platform 1 L Erlenmeyer Flask Dedicated Platform 2 L Erlenmeyer Flask Dedicated Platform Sticky Pad Platform Clamps -- -- -- -- -- 64 34 25 16 9 5 --

() Flask clamps for Universal Platform must be ordered separately. See page 5. (**) Sticky Pad sold separately, see page 5.

Excella E2 Microprocessor Controlled & E1 Analog-Controlled Compact Open Air Shakers

E2: Benchtop shaker for agitating test tubes and flasks up to 500 mL. Speed: 50 - 400 rpm (less for baffled flasks), with electronic speed control. Orbit: 3/4" (1.9 cm). Platform: 13" x 11" (33 x 28 cm), sold separately. Overall dimensions: 15.75" W x 17.9" D x 8.5" H (40 x 45.5 x 21.6 cm). Net weight: 35 lbs. (15.9 kg). E1: Same as E2, but with analog control.

Model Catalog No. M1350-0000 M1350-0001 Electrical Service 120/100V, 50/60Hz 230V, 50/60Hz Model Catalog No. M1354-0000 M1354-0001 Electrical Service 120V, 50/60Hz 230V, 50/60Hz

E2 with LED display, left. Analog-controlled E1, right.

Open Air

E2

Open Air

E1

() Shakers do not include 13"x 11" platforms which must be ordered separately. See below.

Excella E2 & E1 Interchangeable Platforms

13" x 11" (33 x 28 cm) Stainless Steel

Catalog No. M1001-0240** AG2-UT AG2-00 M1001-9920 AG2-10 M1190-9919 M1190-9915 M1190-9916 M1190-9917 M1190-9918 Description Universal Platform Utility Carrier holds a variety of glassware between rubber-cushioned cross bars Utility Tray with non-skid rubber surface Rod Kit for Utility Carrier (one additional rod) 10 mL Erlenmeyer Flask Dedicated Platform 25 mL Erlenmeyer Flask Dedicated Platform 50 mL Erlenmeyer Flask Dedicated Platform 125 mL Erlenmeyer Flask Dedicated Platform 250 mL Erlenmeyer Flask Dedicated Platform 500 mL Erlenmeyer Flask Dedicated Platform Clamps -- -- -- -- 60 32 20 12 8 6

(**) Flask clamps for Universal Platform must be ordered separately. See page 5.

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C-Line Shakers

C76 High-Temperature Water Bath Shakers

Microprocessor-controlled orbital shaker for high-temperature applications. Temperature: 7°C above ambient** to 80° C. Speed: 50 - 300 rpm (less for baffled flasks). Orbit: 1/2" (1.3 cm), dynamically balanced. Features feedback control of temperature and agitation rate, with self-diagnostic status lights for speed, temperature and elapsed time. One-piece seamless bath with rounded corners. Platform: 12" x 16.5" (30.5 x 42 cm) holds flasks to 2 L. (Platform sold separately.) Overall dimensions: 21.4" x 26.9" x 18.5" (54 x 68 x 47 cm). Net weight: 115 lbs. (52 kg).

Model Catalog No. M1248-0002 M1248-0003 Electrical Service 120V, 50/60Hz 230/240V, 50/60Hz

Shown with accessory Plexiglas Gable Cover

C76 Water Bath

() Shaker does not include 12" x 16.5" platforms which must be ordered separately. See below. (**) Elevated temperatures attained with aid of a gable cover using bath liquid with a high boiling point to minimize evaporation. For lower temperatures an optional cooling assembly is available. See below.

C76 Interchangeable Platforms & Accessories

12" x 16.5" (30.5 x 42 cm) Stainless Steel

Catalog No. M1231-9930* M1231-9933 M1231-9934 M1231-9935 M1231-9936 M1231-9937 M1231-9938 M1231-9939 Description Universal Platform 50 mL Erlenmeyer Flask Dedicated Platform 125 mL Erlenmeyer Flask Dedicated Platform 250 mL Erlenmeyer Flask Dedicated Platform 500 mL Erlenmeyer Flask Dedicated Platform 1 L Erlenmeyer Flask Dedicated Platform 2 L Erlenmeyer Flask Dedicated Platform Sub-Platform for mounting half-size platforms containing flasks and test tube racks. Sub-platform holds 2 half-size flask platforms or 4 racks. Clamps -- 31 22 13 8 6 2 --

Half-Size Platforms (Half platforms require Sub-Platform listed above)

Catalog No. AG7-50 AG7-125 AG7-250 Half Platforms Description Per Bath 50 mLErlenmeyer Flask Half Platform 2 125 mL Erlenmeyer Flask Half Platform 2 250 mL Erlenmeyer Flask Half Platform 2 Clamps Per Platform 13 8 5

Test Tube Racks

Catalog No. AG7-TT13 AG7-TT16 AG7-TT20 AG7-TT25 Description Rack for 13 mm Tubes Rack for 16 mm Tubes Rack for 20 mm Tubes Rack for 25 mm Tubes Racks Per Bath 4 4 4 4 Tubes Per Rack 60 36 29 18

Additional Accessories

Catalog No. M1231-2010 M1231-2000 M1231-9920** M1248-0730 M1231-6000 Description Clamps Gable Cover, Plexiglas, minimizes heat loss and heat-up time. Not recommended for temperatures above 60°C. Gable Cover, Stainless Steel, for high-temperature applications up to 100°C. Reduces heat-up time. Minimizes evaporation. Cooling Assembly, factory installed only. Must be ordered with unit for factory installation Spare Set of Arms Spare Parts Kit

(*) Flask clamps for Universal Platform must be ordered separately. See page 5. (**) For below-ambient temperature an optional factory installed cooling coil is available. () Refer to page 2 for details of Spare Parts Kit.

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19

Ultra-Low Temperature (-86°) Freezers

Five Considerations In Selecting an Ultra-Low Temperature Freezer For Your Lab:

The purchase of a ULT freezer is of critical importance to a laboratory. Whereas in the past, models were roughly equivalent, today's freezers can vary considerably in performance, energy consumption and reliability. The ULT freezer is an integral and valuable instrument in the operation of any sample storage application, yet many purchasing decisions are based solely on price. Here are five factors to consider when selecting an ultra-low temperature freezer.

Reliability:

The foremost consideration in a ULT freezer purchase is reliability. The value of products stored in this type of unit can rapidly approach millions of dollars, especially when the effort and critical nature of the samples are factored. Yet, many laboratories continually store these valuable samples in units of inferior quality. This is equivalent to hanging a masterpiece on a needle. It may work for a while, but ultimately your sample's integrity will be compromised. It is important to look at how these units are built; the quality of the compressors, the construction of the door and gaskets, and the operation of the control system. These key system components have a profound effect on the reliability of the system.

Performance:

The second component to consider is performance. The time it takes for a unit to recover after a door opening, the pull down time to get to the temperature set-point, and the temperature uniformity are all vital features of the conditions that the samples are subjected daily. A unit incapable of minimizing these frequent swings in temperature will inevitably cause sample degradation.

Efficiency:

A third measure to consider, and one becoming more valuable by the day, is the energy efficiency of the unit. ULT freezers are traditionally high energy consumers requiring frequent compressor cycling for optimal performance. It is important to look at cycling times, and more importantly kW/h consumption rates, to gain a clear understanding of what the freezer is costing the facility. With energy costs soaring, the operating efficiency difference between manufacturers can be significant. Secondary to the equation is the environmental impact these freezers have on our environment. A high efficiency model can not only benefit the organization financially, but also benefit the global environment by lowering its carbon footprint.

Footprint:

The footprint of the unit is a fourth consideration. How many samples can be stored and how much space does the unit consume? Costs per square feet for laboratory space is extremely expensive and most laboratories are space-limited. The ULT freezer should be able to seamlessly integrate into the area without becoming obtrusive. In addition, stringent safety guidelines require specific walk through and spatial zones in which a traditional freezer may not suffice.

Customer Service:

It's important to recognize the level of customer service being offered. If there is a problem or you have a question, how quickly is there a response? How are the lead-times of the units? You should not have to wait months for a unit to arrive. How well is a service call handled? It may initially seem like a small component, but if there is a problem with the unit, you want a partner, not a supplier.

When these five key features are taken into consideration during the selection process, along with value, it becomes clear that an NBS -86° ULT Freezer is the intelligent choice for your laboratory.

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Environmentally Friendly & Energy Efficient Freezers

All NBS Freezers are exceptionally energy efficient. Our freezers consume roughly half the power of competitor's ULT freezers of similar size, saving you thousands of dollars in energy bills over the life of the freezer. How do we do it? · Specialized low-temperatue seals on outer & inner doors prevent cold air loss · Insulated inner doors & sub-lids reduce temperature fluctuation · New ice-proof inner door latches minimize cold air loss (Innova models only) · Multiple compartments reduce cold air loss when the outer door is opened · Use of a single, powerful fan (most freezers require two) reduce energy consumption · Two-stage cascade refrigeration system provides efficient circulation of refrigerants · Heavy-duty compressors reduce cycle times, to significantly reduce energy usage · Filter-cleaning indicator prevents compressor overheating How much can you save over the life of your freezer? See www.nbsc.com/freezers NBS Freezers are Eco-Friendly · No CFCs (chlorofluorocarbons) or HCFCs (hydrochlorofluorocarbons) are used, to reduce emission of greenhouse gasses, as well as minimize depletion of the ozone layer · Four new HFC-Free "Green" Models are now offered. · NBS freezers are 95 - 98% recyclable (by weight) · And they meet all WEEE Directives for Disposal, as set by the European Union

Which Model is Right For You? · Space-Saving Innova Freezers - utilize Vacuum Insulation Panel technology to provide up to 30% more internal capacity than freezers of equal size. Seven models are offered. See pages 22-23. · Economical Premium Freezers - these conventionally-insulated models provide a cost-effective alternative, with many of the same features and quality as the Innova models. See page 24. · NEW HFC-Free Green Freezers safeguard your samples and the environment. Currently available in 50Hz models only. See below for details.

New Eco-Friendly "Green Freezers", Innova U725-G & Premium U570-G Upright Models

NBS is proud to offer our first hydrocarbon-based refrigerant freezers, to further help prevent global warming. These models are identical in all respects to our energy-efficient and eco-friendly Innova U725 & Premium U570 freezers shown on pages 22-24, except that in addition to eliminating all use of CFCs and HCFCs, these models are also free of hydrofluorocarbons (HFCs): · HFCs are a known greenhouse gas and contributor to global warming. · By switching to hydrocarbon propane/butane based refrigeration system, NBS is able to offer the most environmentally friendly, energy efficient ULT freezer on the market, while maintaining safety and reliability. · Water-cooled options are available for even more energy and environmental savings.

Model Catalog No. Electrical Service 230V, 50Hz 230V, 50Hz

Innova U725-G

Innova U725-G Upright Premium U570-G Upright

U9440-0005 Air-Cooled U9440-0004 Water-Cooled

U9270-0005 Air-Cooled U9270-0004 Water-Cooled

230V, 50Hz 230V, 50Hz

() These models are currently only available with 230 V, 50 Hz power and are supplied with both UK and Schuko cordsets.

Premium U570-G

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21

Innova® Ultra-Low Temperature (-86°C) Freezers

Innova® ­86°C Freezers with Space-Saving Vacuum Insulation Panel Technology

Energy-efficient, quiet running and dependable, Innova Freezers use space-saving Vacuum Insulation Panel technology to reduce wall thickness for a significant increase in storage capacity. Advanced features include: · New gasketed, insulated inner doors that lift-off without tools for easy cleaning · Heated air vent with ice-clearing plunger to allow doors to be re-opened anytime · Easy-to-read LED display with display of system alarms & diagnostics · Internal Voltage Stabilizer including Buck/Boost · Advanced security: four-digit password protects temp. and alarm settings; keyed door lock with optional padlock adapter; lockable security plate protects power and alarm on-off switches; and remote alarm contacts for connection to your external building alarm/monitoring system. · CE, UL & WEEE certifications. Innova Models

U101 U360 U535 U725 & U725-G C585 C760

Style

Upright Upright Upright Upright Chest Chest

Capacity Max. Box Capacity Liters Cubic Ft. 2" 3" 4"

101 360 535 725 585 760 3.6 12.7 18.9 25.6 20.7 26.9 60 252 336 504 416 572 36 180 240 360 288 396 24 108 144 216 192 264

Dimensions (H x W x D) Internal External

32.7" x 35.4" x 22.3" 83 x 90 x 56.6 cm 76.8" x 26.4" x 34.1" 195 x 67.0 x 86.7 cm 76.8" x 31.5" x 34.1" 195 x 80 x 86.7 cm 76.8" x 40.4" x 34.1" 195 x 102.5 x 86.7 cm 43" x 66.5" x 30.9" 109.2 x 169 x 78.5 cm 43" x 80.7" x 30.9" 109.2 x 205 x 78.5 cm 25.2" x 18.9" x 13" 64 x 48 x 33 cm 53.7" x 17.3" x 24.2" 136.5 x 44.0 x 61.5 cm 53.7" x 25.2" x 24.2" 136.5 x 64 x 61.5 cm 53.7" x 34.1" x 24.2" 136.5 x 86.5 x 61.5 cm 30.7" x 47.2" x 24.6" 78 x 120 x 62.5 30.7" x 61.4" x 24.6" 78 x 156 x 62.5 cm

() To allow for handles and hinges, add 3.1" (80 mm) to width of upright freezers and 4.3" (110 mm) to the depth of chest freezers.

Innova Upright & Chest Models

Six space-saving Innova models to choose from: · Two unique upright sizes - U101 fits on or under bench. Ultra-slender U360 fits where other models simply can't go. (See page 23 for details). · Two Larger Uprights: For easy access to samples, choose the U535 or for more capacity, the larger U725, each with three compartments and two adjustable-height shelves. · Two Chests: If a chest style is preferred, choose C585 or C760. Sub-lids provide added insulation. · CO2 and LN2 Back-up systems available - see page 25.

Model Catalog No. Electrical Service 120V, 60Hz* 208/220V, 60Hz 230V, 50Hz

Innova U535 Upright Innova U725 Upright Innova C585 Chest Innova C760 Chest

U9430-0000 U9430-0002 U9430-0001

U9440-0002 U9440-0001

208/220V, 60Hz* 230V, 50Hz

U9400-0000 U9400-0002 U9400-0001

120V, 60Hz* 208/220V, 60Hz 230V, 50Hz

U9410-0002 U9410-0001

208/220V, 60Hz 230V, 50Hz

(*) Dedicated 20 Amp line required for 120 V freezers () All 50 Hz freezers are supplied with both UK and Schuko cordsets. () See also HFC-free Green-model U725-G on page 21 & 24. (For use in Europe only.)

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Innova U101 Personal-Sized, -86°C Upright Freezer On or Under Lab Bench.

Why store your valuable samples down the hall, when you can have the convenience and security of a personal-sized, 3.6 cu. ft (101 L) upright freezer tucked on or under your lab bench? · Integrated key lock on handle · Two inner doors prevent cold air loss; lift off for easy cleaning · Capacity: Hold up to 6000 samples (60 two-inch boxes) · Stackable · Rear wheels provide easy mobility Front-opening door · All the advantages of our other Innova models. for easy access

to all samples

Model

Catalog No. U9420-0000 U9420-0001

Electrical Service 120V, 60Hz* 230V, 50Hz

Innova U101 Upright

Innova U360 Ultra-Slim Upright Freezer with Advanced Control & Monitoring

Ultra-slender, 12.7 cu. ft. (360 liters), the Innova upright U360 offers all the advantages of our other Innova freezers, plus an advanced new controller with: · Large, back-lit display (4.75" x 2.7" [12 x 6.8 cm]) for on-screen monitoring, programming & data graphing · Automated data logging of temperature, alarms and door openings · User-programmable setpoints, alarms & passwords - Programmable alarm delays, with log showing alarm acknowledgments · On-screen messaging & diagnostics Three compartments with insulated, gasketed lift-off inner doors, and an advanced new low noise, energy saving refrigeration system are a few of the other advantages.

Model Catalog No. U9425-0000 U9425-0001 Electrical Service 120V, 60Hz* 230V, 50Hz

Just 26.4" wide (67 cm) including the handle!

Innova U360 Upright

(*) Dedicated 20 Amp line required for 120 V freezers () All 50 Hz freezers are supplied with both UK and Schuko cordsets

Innova U101 & U360 Accessories (More freezer accessories on pages 25 & 26.) Energy Savings Calculator

Catalog No. Description STACKING KIT for Model U101 P0625-0020 U101 Stacking kit, provides structure for safe stacking INTERNAL VOLTAGE STABILIZERS M1322-0000 External Voltage Stabilizer, 120V, 60 Hz, Model U101 P0625-1090 Internal, Slide-In Voltage Stabilizer, 120V, 60Hz, Model U360 AIR FILTERS K0200-0506 Model U101, washable, spare P0625-1110 Model U360, washable, spare

() CO2 and LN2 Back-up systems available - see page 25.

How many thousands of dollars can you save over the life of your freezer? See www.nbsc.com/freezers.aspx for an energy savings worksheet

U101

U360

U535

U725

C585

C760

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23

Premium Ultra-Low Temperature (-86°C) Freezers

Premium ­86°C Ultra-Low Temperature Freezers with Traditional Urethane Foam Insulation

Insulation: conventional urethane foam insulation. Display and Control: easy-to-read LED display; S.M.A.R.T. PLUSTM controller presents error and alarm codes and detailed systems information. Advanced Features: NEW vent plunger; heated air vent prevents vacuum formation; keyed lock; user-defined password protection; stainless-steel interior; 4 shelves included in upright models; removable power cords and numerous options and accessories. Premium Models

U410 U570 & U570-G C340 C660

Style

Upright

Capacity Liters Cubic Ft.

410 14.5

Max. Box Capacity 2" 3" 4"

240 180 120

Dimensions (H x W x D) External Internal

75.4 x 31.5 x 33.5 191.5 x 80 x 85.2 cm 4.8" x 21.7" x 22.6" 126.5 x 55 x 57.5 cm 49.8" x 30.1" x 22.6" 126.5 x 76.5 x 57.5 cm 30.0" x 30.0" x 23.2" 76 x 76 x 59 cm 30.0" x 57.9" x 23.2" 76 x 147 x 59 cm

Upright Chest Chest

570 340 660

20 12 23.3

400 260 520

300 180 360

200 120 240

75.8 x 40.3 x 33.5 192.5 x 102.5 x 85.2 cm 42.3 x 52.8 x 33.1 107.5 x 134 x 84 cm 42.3 x 80.7 x 33.1 107.5 x 205 x 84 cm

() To allow for handles and hinges, add 3.1" (80 mm) to width of upright freezers and 4.3" (110 mm) to the depth of chest freezers.

Premium Upright & Chest Models

Four space-saving Premium models to choose from: · Two Uprights: For easy access to samples, choose the U410 or U570, each with five compartments and four adjustable-height shelves. · Two Chests: If a chest style is preferred, choose C340 or C660. Two insulated panels provide added insulation. · CO2 and LN2 Back-up systems available - see page 25.

Model Catalog No. U9260-0000 U9260-0002 U9260-0001 U9270-0000 U9270-0002 U9270-0001 Electrical Service 120V, 60Hz* 208/220V, 60Hz 230V, 50Hz 120V, 60Hz* 208/220V, 60Hz 230V, 50 Hz

Heated Vent Plunger Now Standard!

Premium U410 Upright Premium U570 Upright Premium C340 Chest Premium C660 Chest

U9230-0000 U9230-0001

120V, 60Hz* 230V, 50Hz

U9250-0002 U9250-0001

208/220V, 60Hz 230V, 50Hz

(*) Dedicated 20 Amp line required for 120 V freezers () All 50 Hz freezers are supplied with both UK and Schuko cordsets

U410

U570

C340

C660

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New Brunswick Scientific · a culture of innovation · www.nbsc.com

Freezer Accessories, All Models

CO2 & LN2 Freezer Backup Systems^

Totally-independent systems utilize their own temperature probe and battery backup to provide protection even during a power outage. Catalog No. U9043-0002 U9043-0004 U9044-0002 U9044-0004 Description Innova Freezers: CO2 Back-up, 120 - 220V, 60Hz CO2 Back-up, 230V, 50Hz LN2 Back-up, 120 - 220V, 60Hz LN2 Back-up, 230V, 50Hz Catalog No. U9043-0006 U9043-0008 U9044-0006 U9044-0008 Description Premium Freezers: CO2 Back-up, 120 - 220V, 60Hz CO2 Back-up, 230V, 50Hz LN2 Back-up, 120 - 220V, 60Hz LN2 Back-up, 230V, 50Hz

(^) NBS recommends that all back-up systems be factory installed.

Freezer Accessories (More accessories on page 26 - 27)

Catalog No. K0440-0355 K0540-0025 K0660-0051 K0280-1031 K0280-1030 P0625-1180 K0280-1034 K0280-1036 P0625-1170 M1322-0000 M1322-0002 M1291-0054 Description 7-Day Chart Recorder 7-Day Chart Recorder Chart Recorder Paper, pack of 100 charts Chart Recorder Pens, pack of 3 Shelves Extra Shelf, Premium Model U410 Extra Shelf, Premium Model U570 Extra Shelf, Innova Model U360 Extra Shelf, Innova Model U535 Extra Shelf, Innova Model U725 Padlock Adapter Kit: All upright models Kit allows up to two user-supplied padlocks to be secured to outer door handle for extra security. External Voltage Stabilizer: for Premium and U101 Freezers only External Voltage Stabilizer, 120V, 60Hz External Voltage Stabilizer, 208/220V, 60Hz Automated Data Logging & Control Software BioComand® SFI - Automated Data Logging & Controller Software (see page 32).

() All shelves provided with 4 shelf clips for installation.

Inventory Racking Systems, Innova & Premium

Lightweight for easy transport, yet durably constructed of non-corrosive aluminum, our freezer racking is exclusively designed for New Brunswick Scientific freezers to maximize storage space; and provide one of the lowest costs per capacity in the industry. Racks for upright freezers feature drawers that pull forward for easy accessibility to your samples. Standard racking holds boxes of various sizes, or we can supply racks to any specification.

NBS Plastic-Coated Cardboard Boxes

How To Select Racking Accessories

Step 1. Select your box size in the chart to the right. Step 2. Choose the correct divider for your tube/vial diameter. Step 3. Choose the appropriate rack to match your box size from the chart on page 27.

1.

Max Tube Choose Catalog Height Box Dimensions (HxWxD) No. Inches/mm correct Box Inches mm for your B50-SQ 2" 50 5.2 x 5.2 x 1.97" 132 x 132 x 50 mm tube/vial B75-SQ 3" 75 5.2 x 5.2 x 2.76" 132 x 132 x 75 mm Height

B95-SQ 4" 100 5.2 x 5.2 x 3.4" 132 x 132 x 100 mm

Dividers for NBS Plastic-Coated Cardboard Boxes 2.

Choose correct Box Divider for your tube/vial Diameter Maximum Tube Diameter Catalog No.

Inches mm

Matrix

No. of Vials or Tubes

D100 D81 D64 D49

0.46 0.51 0.59 0.69

11.8 13.0 15.0 17.4

10 x 10 9x9 8x8 7x7

100 81 64 49

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25

Innova & Premium Freezer Accessories

®

Racking Capacity Chart for Upright & Chest Freezers 3.

Style Model U101 Innova U360 Innova U535 Innova U725 Innova U410 Premium U570 Premium C585 Innova C760 Innova C340 Premium C660 Premium Max. Racks Per Shelf 3 3 4 6 3 5 N/A N/A N/A N/A Max No. of Shelves 2 3 3 3 5 5 N/A N/A N/A N/A Max. Racks Per Freezer 6 9 12 18 15 25 32 44 20 40

Upright

Chest

External Rack Dimensions (Depth x Width x Height)

Style Upright Model U101 Innova U360, U535 & U725 Innova U410 & U570 Premium Chest ALL Models Inches 12.13 x 5.55 x 11.52 22.24 x 5.55 x 16.16 22.24 x 5.55 x 9.25 5.35 x 5.47 x 29.61 mm 308 x 141 x 292.5 565 x 141 x 410.5 565 x 141 x 235 136 x 139 x 752

Select box size, read down chart to freezer model to find rack part number & price.

Freezer Model 2" or 50 mm

K0641-3003 U101 K0641-3000 U360 U535 K0641-3001 K0641-3002 K0641-3000 U725 K0641-1690 C585 K0641-1690 C760 K0641-1900 U410 K0641-1900 U570 K0641-1890 K0641-1880 K0641-1690 C340 K0641-1690 C660 K0641-1700 K0641-1750 K0641-1700 K0641-1750 K0641-1890 K0641-1880 K0641-1700 K0641-1750 K0641-1700 K0641-1750 K0641-3001 K0641-3002 9 K0641-3004 K0641-3005

Read across chart to find rack and box quantity information for your freezer model

Total Racks per Freezer

6

3" or 75 mm

4" or 100 mm

x x

Boxes per Rack

10 6 4 28 20 12 28 20 12 28 20 12 13 9 6 13 9 6 16 12 8 16 12 8 13 9 6 13 9 6

= =

Total # Boxes per Freezer

60 36 24 252 180 108 336 240 144

12

x

=

18

x

=

504 360 216 416 288 192 572 396 264 240 180 120 400 300 200 260 180 120 520 360 240

32

x

=

44

x

=

15

x

=

25

x

=

20

x

=

40

x

=

26

New Brunswick Scientific · a culture of innovation · www.nbsc.com

ULT Freezer Temperature Monitoring System

A2 Independent Temperature Monitor, Data Logger and Advanced Autodialer for ULT Freezers

The integrity of your samples is paramount to your research. This is why New Brunswick Scientific has partnered with Accsense to offer the all-in-one A2 Temperature Monitoring System. The A2 is an independent temperature monitor with alarm, electronic chart recorder, and an autodialer incorporated in one small pod that integrates seamlessly to the side of your equipment. Independent temperature monitoring: The A2 system consists of an NIST-traceable probe and a small communication pod about the size of a wallet. The A2 communicates through any standard Ethernet connection with internet access. No software to download. Firewall compatible. Setup is fast and easy, taking only five minutes to be installed. Data logging: The A2 transmits data to a professionally managed centralized data server where redundant systems store and record probe, ambient temperature and alarms. A web-based portal allows the user access to this data anytime, from any computer. The intuitive data graphing and tracking functions allow for a complete trace of the installed freezer's performance. Autodialer*, text and e-mail alert system: The integrated e-mail, text, and phone messaging service alerts a sequential number of users to any alarms, until the alarms are recognized. In addition, alarm acknowledgements and resolutions are tracked for regulatory control. Request a demo today!

(*) Currently, autodialer available for U.S. use only. Intuitive, user friendly data interface

NEW!

Features:

· Web-based access to data from anywhere in the world · Secure encrypted transmission · NIST-traceable probe · System setup in less than 5 minutes - simply connect to ethernet · No software to install or maintain · Phone, text and e-mail alarm notification · Historic data recording and report generation · Non-alterable data · Corrective action plans · Independent alarm notifications · Easily retrofitted on current units · Firewall compatible · Wall plug or power over ethernet option · NIST certification available

Model

Catalog No. P0625-1630

Description Pod & Probe with wall plug power connection, 60 Hz Pod & Probe with power over ethernet connection Pod & Probe with wall plug power connection, 50 Hz One-Year Subscription Three-Year Subscription Five-Year Subscription

A2 Pod and Probe

P0625-1640 P0625-1635

Subscriptions

P0625-1650 P0625-1651 P0625-1652

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27

CO2 Incubators - Direct Heat

NEW!

NEW BRUNSWICK GALAXY

®

C02 INCUBATORS

The sophistication required of today's cell culture laboratories is increasing rapidly in light of historic advances in the emerging fields of Stem Cell, IVF, as well as traditional cell culture. The specific requirements for experimentation have gone beyond a "one size fits all" platform to the need for a both robust and highly controllable incubator. These advancements have altered the role of the CO2 incubator into an integral instrument of research advancement. In response to these needs, NBS is launching its newly designed GALAXY brand of CO2 incubators. These units have more standard features, more options, and the addition of a full featured economical unit, the CO-48S. By responding to the rapidly growing needs of cell culture, NBS has the CO2 incubator to drive today's research as well as the future demands of your laboratory.

Standard Features

· Direct Heating System: A unique heating profile uses natural convection to gently bathe cells in warm air. This not only allows for a superior uniformity but also guards against the rapid fluctuations seen with traditional fan-driven incubators which can cause drastic changes in genotypic and phenotypic expression of cells. · Fanless Design with Seamless Deep Drawn Chamber: By eliminating the fan and housing, the Galaxy incubator is able to provide the maximal usable space in the chamber. There is no increase in desiccation on the top shelf so your samples are uniform throughout the chamber. In addition, the fanless design removes a historical hotspot of contamination as well as eliminating the need for expensive internal HEPA filters. This, combined with the seamless chamber, leaves contaminants no place to hide. · Advanced Display and Controller: The large 5.3" (13.5 cm) display on the NBS R Series incubators allows for comprehensive and rapid analysis of the performance of the unit and incorporates user-friendly intuitive controls. In addition, these models offer 72 hour on board graphing capability of temperature, gas, and alarms. · IR CO2 SENSOR: The Infra-Red CO2 sensor provides superior accuracy and stability over traditional thermo-conductivity sensors. · NEW - Sealing Inner Glass Door: Standard on all CO-170 models. Engineered to offer a more rapid gas and temperature recovery, and allow users the ability to visualize their cultures while maintaining critical atmosphere. · NEW - 25mm Access Port: Standard on all CO-170 and CO-48 models, the access port allows for additional probes and equipment to be used inside the incubator. · NEW - RS232 Port: The RS232 communications port allows for data offloading and logging to a PC. · NEW - Perforated Shelves: The newly designed perforated shelves offer increased performance in temperature, gas and humidity recovery.

Options

· High Temperature Decontamination: One touch 120° C 4 hour sterilization cycle for enhanced contamination protection. Available on the CO-170R, S and CO-48R, S · Oxygen Control: Three levels of oxygen control to provide a hypoxic or hyperoxic environment. Most common option is ideal for the 1-19% O2 majority of hypoxic applications Option for strict hypoxic conditions. 0.1-19% O2 Tri-gas option for both hypoxic and 1-95% O2 hyperoxic studies. Applications: Stem Cell, IVF, In Vivo Mimicking Available on the CO-170R, CO-48R, and CO-14S · NEW - Cooling Option: Improved Cooling option using a Peltier cooling unit to provide the ability to culture cells at or 10°C below ambient temperatures. Applications: Plant Cell, Biofuels Available on the CO-170R · NEW - Active Humidification System: (AHS) allows you to set and maintain humidity using non-condensing humidification. This option also provides a superior humidity recovery and humidity stability and prevents desiccation. Applications: Low Volume, Microtiter Plate Culture Available on the CO-170R · NEW - Active UV Protection: The UV protection guards against contamination using a UV module external to the chamber to provide constant sample protection and no UV risk to the cell cultures. Applications: ALL Available on the CO-170R · Humidity Monitoring Package: The humidity package features a humidity sensor, display and humidity tray warning system for additional monitoring and control. Applications: ALL Available on the CO-170R and CO-48R

28

New Brunswick Scientific · a culture of innovation · www.nbsc.com

Galaxy CO-170 Series, Full-Sized CO2 Incubator, 170 Liter 6.0 cu. ft. Capacity

The CO-170 Series offers the advanced LCD display with on board 72 hour data logging. Standard Features: include an IR CO2 Sensor, sealed inner glass door, RS232 communication port, and a 25mm Access port. The integrated LCD controller allows simple on screen programming of alarms and settings. The R series allows for the full range of options including O2 control, Active Humidification, High Temperature Decontamination, Active UV sterilization and cooling. The Galaxy CO-170 series of incubators offers 6.0 cu. ft. of a highly controlled incubation environment and a tremendous amount of usable space all in a conveniently small footprint. These units are stackable with stand, and shelving is easily removable without tools for a rapid wipe down.

170L Incubator, R 170 to Choose From: Three Models IncubIatorof and SLSeries: R Series: The full featured R unit including a large 5.3" (13.5 cm) intuitive controller with on-board data logging and simple access to alarms and settings and allows complete access to the full range of upgrades including the new AHS and Cooling modules as well as O2 control and High Temp Decontamination. S Series: A full featured unit with an LED control system and High Temperature Decontamination. Volume: Shelves: Specifications 170L, 6.0 cu. ft. 4 Shelves

NEW!

External Dimensions: 27" W x 26.7" D x 33.2" H, (68.7 x 68 x 84.4 cm) Internal Dimensions: 21" W x 17.5" D x 27.2" H, (54 x 44.4 x 69.3 cm) Weight: Range: Uniformity: Certification: 183 lbs. (83 kg) 4°C above Ambient to 50°C +/- 0.2°C CE

Model

Catalog No. for 120V CO170R-120-0000 CO170R-120-1000 CO170R-120-0200 CO170R-120-1200

Catalog No. for 230V CO170R-230-0000 CO170R-230-1000 CO170R-230-0200 CO170R-230-1200

Options

CO-170R

High Temp Decontamination 1-19% O2 Control High Temp Decontamination, 1-19% O2 Control

CO-170S

CO170S-120-0000 CO170S-120-1000

CO170S-230-0000 CO170S-230-1000

High Temp Decontamination

CO-170 R large back-lit displays provide a detailed record of chamber conditions - including an automated 72-hr. record of historical data.

For more options and configurations, see page 31.

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29

CO2 Incubators - Direct Heat

Galaxy CO-48 Series, Personal-Sized CO2 Incubator, 48 Liter 3.6 cu. ft. Capacity

NEW!

The Galaxy CO-48 is specifically designed for smaller laboratories and applications requiring a higher level of isolation. Providing many of the same standard features as the CO-170 and including a viewing window on the outside door, the CO-48 offers sophisticated performance in a smaller package. New for 2009 is the Galaxy CO-48S series providing a more economical choice in a convenient size.

48L Incubator, R and S Series: Volume: R Series: Offers large 5.3 LCD intuitive interface with 72 hour data logging and easy access to set points and alarms. This unit is upgradeable with many options including O2 control and High Temperature Decontamination. S Series: Offers an LED controller with optional High Temperature Decontamination. Shelves: Specifications 48 L, 3.6 cu. ft. 3 Shelves

External Dimensions: 19" W x 18.7" D x 25.4" H, (48.4 x 47.5 x 64.5 cm) Internal Dimensions: 15.8" W x 12" D x 15.8" H, (40.1 x 30.8 x 40.1 cm) Weight: Range: Uniformity: Certification: 32 (71 kg) 4°C above Ambient to 50°C +/- 0.2°C CE

Model

Catalog No. for 120V CO48R-120-0000 CO48R-120-1000 CO48R-120-0200 CO48R-120-1200

Catalog No. for 230V CO48R-230-0000 CO48R-230-1000 CO48R-230-0200 CO48R-230-1200

Options

CO-48R

High Temp Decontamination 1-19% O2 Control High Temp Decontamination, 1-19% O2 Control

CO-48S

CO48S-120-0000 CO48S-120-1000

CO48S-230-0000 CO48S-230-1000

High Temp Decontamination

Galaxy CO-14 Series, Micro-Sized CO2 Incubator, 14 Liter 0.5 cu. ft. Capacity

NEW!

The unique CO-14, originally designed for the IVF market, is ideal for isolation of sensitive cultures. Its compact size allows the unit to be placed directly in your work area and its 0.5 cu.ft. capacity allow for an enhanced uniformity and temperature specification.

14L Incubator, S Series: Volume: S Series: The CO-14 standard S Series offers compact CO2 culture capability in a package that does not intrude on your valuable laboratory space. Also available with a 1-19% O2 control option ideal for IVF and stem cell applications. Shelves: Specifications 14 L, 0.5 cu. ft. 2 Shelves

External Dimensions: 12.3" W x 11" D x 17.8" H, (31.3 x 28.5 x 45.1 cm) Internal Dimensions: 9.2" W x 8.2" D x 11.5" H, (23.3 x 20.8 x 29.2 cm) Weight: Range: Uniformity: Certification: 13.5 (6 kg) 4°C above Ambient to 50°C +/- 0.2°C CE

Model

Catalog No. for 120V CO14S-120-0000 CO14S-120-0200

Catalog No. for 230V CO14S-230-0000 CO14S-230-0200

Options

CO-14S

1-19% O2 Control

30

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CO2 Incubator Options, Factory Installed

Incubator Options

Standard Unit High Temperature Decontamination 1-19% O2 Control High Temperature Decontamination & 1-19% O2 Control BMS Alarm Relays 0.1 - 19% O2 1 - 95% O2 Peltier Cooling Active Humidification Active UV Decontamination Humidity Monitoring Package Copper Chamber Inner Door 4-Split Inner Doors 8-Split Inner Doors 2-Split Inner Doors IPP Internal Power Receptacle P0628-5560 P0628-5560

Models

CO-170R

CO170R-120-0000 CO170R-230-0000 CO170R-120-1000 CO170R-230-1000 CO170R-120-0200 CO170R-230-0200 CO170R-120-1200 CO170R-230-1200

CO-170S

CO170S-120-0000 CO170S-230-0000 CO170S-120-1000 CO170S-230-1000

CO-48R

CO-48S

CO-14S

CO14S-120-0000 CO14S-230-0000

CO48R-120-0000 CO48S-120-0000 CO48R-230-0000 CO48S-230-0000 CO48R-120-1000 CO48S-120-1000 CO48R-230-1000 CO48S-230-1000 CO48R-120-0200 CO48R-230-0200 CO48R-120-1200 CO48R-230-1200

CO14S-120-0200 CO14S-230-0200

P0628-5540 P0628-5410 P0628-5400 P0628-6810 P0628-6800 P0628-6790 P0628-6820 P0628-5612 STANDARD P0628-6780 P0628-6781

P0628-5540

P0628-5340 P0628-6280 P0628-5260

P0628-5340

P0628-6300

P0628-6770 P0628-5612 STANDARD P0628-6780 P0628-6761 P0628-5350 P0628-6761 P0628-5350 P0628-6760 P0628-6760 P0628-6210

CO2 Incubator Accessories

Incubator Accessories

CO2 Bottle Automatic Change-Over Controller CO2 Supply Line Filters, HEPA (2) In-Line Pressure Regulator Auto Zero Filters, HEPA, (Pack of 5) CO2 Gas Analyzer Kit 10 Spare CO2 Gas Analyzer Tubes BioCommand® SFI Double Stacking Stand Below Bench Caster Set Additional Shelf, Perforated Multi-Position Wire Shelf Rack Additional Humidity Tray Electronic CO2 Gas Analyzer O2 Sensor (spare)

CO-170

P0628-5000 P0628-5020 P0628-5030 P0628-5060 P0628-5040 P0628-5050 M1291-0054 P0628-6270 P0628-6160 P0628-6251 STANDARD P0628-6260 (2.5 Liter) P0628-6150 P0628-5790

CO-48

P0628-5000 P0628-5020 P0628-5030 P0628-5060 P0628-5040 P0628-5050 M1291-0054 P0628-5090 NA P0628-5080 P0628-5100 P0628-5940 (0.5 Liter) P0628-6150 P0628-5790

CO-14

P0628-5000 P0628-5020 P0628-5030 NA P0628-5040 P0628-5050 M1291-0054 P0628-6230** NA NA P0628-6170 P0628-6200 (0.3 Liter) P0628-6150 P0628-5790

Note: Certain option combinations may not be possible. Please consult your NBS sales representative for assistance selecting the right combination for your lab.

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31

Automated Data Logging & Control

NEW!

BIOCOMMAND® SFI LABORATORY CONTROL SOFTWARE

Enhance the Power and Control of your Shakers, CO2 Incubators and Freezers: BioCommand SFI has taken the advanced control of the BioCommand supervisory software and optimized the features and design specifically for standard laboratory equipment including Shakers, CO2 Incubators and ULT Freezers*. This software gives the operator advanced control and data trending capability for not only New Brunswick units but any OPC-compatible unit. Trend and control your entire lab through a single PC. Features: · Design and save templates setting specific growth conditions on Shakers and CO2 Incubators. · Monitor and control multiple units from a single PC. · Trend and archive critical data including temperature, gas, RPM, etc, up to 32 units simultaneously. · Graphical representation of output. · Provides localized control and monitoring with alarms of integral laboratory equipment. · Visible and Audible Alarm including comprehensive alarm log for regulatory operation. · Ability to log any lab equipment that communicates through an OPC protocol.

(*) Units must have RS232, RS422 or USB port.

Model

Catalog No.

BioCommand SFI

Shaker Cable Freezer Cable CO2 Cable

M1291-0054 BioCommand SFI Software M1291-8021 Cable for Shakers M1291-8022 Cable for Freezers M1291-8023 Cable for CO2 Incubators

For BioCommand Plus, BioCommand Plus with OPC or BioCommand Lite see page 66.

Roller Drums

Tissue/Microbial Culture Roller Drum, TC-7 & TC-8

Precision tool designed for the growth of tissue and bacterial cultures by the roller tube method. Interchangeable Drums: accommodate 13 mm, 16 mm, 18 mm & 25 mm test tubes and 50 mL centrifuge tubes. (Drums sold separately.) Speed: 6 to 56 rpm in Model TC-7 and 0.2 to 3.5 in Model TC-8. Overall dimensions: 15" W x 13.5" D x 18" H max. (Height varies dependent on angle adjustment; 37.4 x 34 x 46 cm). Net weight 16 lbs. (7.3 kg).

Model Catalog No. M1053-4004 M1053-4005 M1053-5004 M1053-5005 Speed (RPM) 6 - 56 6 - 56 0.2 - 3.5 0.2 - 3.5 Electrical Service 120V, 50/60Hz 220/230V, 50/60Hz 120V, 50/60Hz 220-230V, 50/60Hz

TC-7 TC-8

Interchangeable Roller Drums, 14" (36 cm) diameter

Catalog No. ATC-TT16 M1053-0450 ATC-TT25 M1053-0360 M1053-0306 Description Holds 164 test tubes 16 mm and smaller Holds 164 test tubes 18 mm and smaller Holds 80 test tubes 25 mm and smaller Holds 46 size 50 mL centrifuge tubes, 30 mm and smaller Holds 311 test tubes, 13 mm diameter x 100 mm length

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New Brunswick Scientific · a culture of innovation · www.nbsc.com

Media Sterilizer & Plate Pourer

MediaMatic® MM9 Benchtop Media Sterilizer

Sophisticated, versatile media sterilizer, benchtop autoclave and controlled water bath. Volume: prepares from 1 to 9 liters of media in about an hour. Features: simple operation combined with 45 storable programs and two-speed stirring provide for controlled production of media and agar. Advanced Features: Built-in printer provides record of all production parameters. Headplate opens without tools or removing bolts - just twist open! Sterile filtered air vent. Uniquely flexible machine can be used for media preparation, general autoclaving for flasks and controlled temperature water-bath applications. Overall Dimensions: 19.3" W x 22.5" D x 17.2"H (49 x 57 x 43.7 cm). Net weight: 108 lbs, (49 kg). Certifications: CE & UL.

Model Catalog No. M1325-0000 M1325-0002 Electrical Service 120/240V, 60Hz 230V, 50Hz

MediaMatic

Connects easily to PourMatic for complete Media Prep System!

MediaMatic Accessories

Catalog No. P0626-0010 P0626-0020 P0626-0030 P0626-0040 P0626-0050 P0626-0100 P0626-0060 P0626-0080 P0626-0070 P0626-0090 P0626-0091 M1325-2210 Description Inner Vessel, stainless steel Magnetic Stirrer Sterile Air Filter, 0.45µM Seal for Vessel Lid, replacement Dispense Fitting for media withdrawal Base Plate & Flexible PT 100 Temperature Sensor (required when autoclaving flasks) Dispense Tube (decanting), silicone Dispense Tube (decanting), stainless steel Printer Paper Roll, 1 per pack Temperature Sensor, double PT 100 flexible Temperature Sensor, double PT 100 rigid Cooling Water Regulator Kit

PourMatic® Model MP-1000 Automated Plate Pouring System

Dependable, automated petri dish pouring system. Capacity: holds up to 320 petri dishes (100 x 15 mm, or 60 x 15 mm) with a dispensing capacity of 5-40 mL. Pours and stacks 320 plates in about 20 minutes. Features: simple dispense line connection using the Masterflex EZ Load Pump head. Advanced Features: optional conversion kits allow easy conversion of 100 mm plate instrument to 60 mm plates and vice versa. A coding ink jet printer is available for printing directly onto the plates. Contact your local NBS sales representative for details. Overall Dimensions: 20.5" W x 24" D x 34"H (52 x 61 x 86.4 cm). Net weight: 80 lbs. (36.3 kg). Certifications: CE & cETL

Model Catalog No. M1224-0002 M1224-0006 Electrical Service 120V, 60Hz 220V, 50Hz 120V, 60H 220V, 50Hz Plate Size 100 mm 60 mm

For 60 or 100 mm plates. Connects easily to MediaMatic for a complete Media Prep System!

PourMatic

M1224-0001 M1224-0005

PourMatic MP-1000 Accessories

Catalog No. P0160-4440 M1224-3000 P0300-0350 P0740-2505 M1224-0160 M1224-0100 M1224-0110 M1224-9200 M1224-9740 P0620-6430 M1240-1000

Description Spring Clamp for securing tubing Dispensing Line Kit, includes stainless steel plug-in deflecting nozzle, 12 ft. (3.7m) silicone tubing, 2 spring clamps UV Lamp, 4 watts (spare) Silicone Tubing, 25 ft. (7.6m) 4.8 mm i.d. Conversion Kit from 100 mm to 60 mm plates Conversion Kit from 60 mm to 100 mm plates Dual Nozzle Kit for Pouring Bi-Plates comes complete with one additional Masterflex quick-load pump head Dispensing Nozzle, stainless-steel (spare) Foot Pedal Pump Switch, factory installed Ink Jet Printer (Requires bracket assembly below), installation & training also available Bracket Assembly for Ink Jet Printer (must be used with printer above)

1.732.287.1200 · 1.800.631.5417 · Fax: 1.732.287.4222 · [email protected]

33

Eppendorf Products Showcase

Eppendorf products support users in a vast number of laboratory settings and tasks. Flagship Eppendorf pipettes, centrifuges and the famous Eppendorf Tube have spurred the evolution & advancements of biotechnology. On the following pages we present a selection of the Eppendorf product portfolio. For details, ordering information, or to view more Eppendorf products, please see their website at: www.eppendorf.com.

Eppendorf Research® Pipettes

Description Separate tip ejection (two-button operation) and certified ergonomics make Eppendorf Research® pipettes lab favorites. They are available in fixed-volume, adjustable-volume, multichannel & electronic versions. Unlike other pipettes that

provoke unnatural hand position during pipetting, the easy-togrip handle & well-placed operating buttons, combined with a perfectly positioned hand rest, ensure an optimal fit.

MixMate®, Thermomixers and ThermoStat plus

MixMate

Thermomixer comfort

Thermomixer compact

ThermoStat plus

Description

The Eppendorf mixer portfolio, including temperature control, is ideally suited for mixing and incubating small volumes. 12 different exchangeable thermoblocks for different types of vessels (plates and tubes) support flexibility of choice to meet individual needs during your daily lab routine. Importantly, the Eppendorf MixMate has demonstrated superior performance, especially during mixing of minute volumes in all kinds of plates. When there is a need for both mixing and temperature control, Thermomixer comfort and Thermomixer compact are the devices of choice. The ThermoStat plus can be used to heat up samples to 99°C and cool them down to as low as -5°C in all commonly used small laboratory vessels.

34

For ordering and more products see www.eppendorf.com

Centrifuges & Micromanipulation

Eppendorf Microcentrifuges

Centrifuges

MiniSpin®

MiniSpin® plus

5418

5424

5415 R

5430

5417 R

Description Microcentrifuges from Eppendorf cover a vast range of applications, starting with a 12-place personal microcentrifuge spinning at 12.100 x g, all the way up to the latest versatile 30- place microcentrifuge with a max. speed of 30.130 x g.

These centrifuges can accommodate the following tubes and plates: 0.4 ml-2.0 ml tubes, PCR tubes, spin columns, Cryo tubes, 15ml/50ml Falcon® (conical) tubes as well as MTP plates.

Eppendorf Multipurpose Centrifuges

Centrifuges

5702

5702 R

5702 RH

5804

5804 R

5810

5810 R

Description With the 5702 series Eppendorf provides an economical centrifuge range for clinical labs, for molecular biology and human medicinal applications. The 5804 / 5810 series, in turn, are general purpose centrifuges for a wide range of applications

and are able to spin plates, tubes and bottles in a volume range of 0.2 ml up to 400 ml. A large array of accessory rotors, buckets and adapters make for unparalleled application versatility.

Eppendorf Micromanipulation Applications

Description Eppendorf offers an exciting range of instruments and consumables for micromanipulation applications. At the forefront, the TransferMan® NK 2 micromanipulator is used for suspended cell applications. The device features three motors, centrally controlled by one very-easy-to-use joystick, for performing all movements, whether fine or broad stroke. With a resolution of 40 nm and the ability to store positions and user profiles, it simplifies even the most complicated microinjection techniques.

35

Tubes, Pipette Tips & Deepwell Plates

Safe-Lock Eppendorf Tubes

®

Description The famous original Eppendorf Tubes ensure absolute safety when working with expensive, toxic or radioactive substances. The hinged lid eliminates any danger of leaks. The 0.5 ml, 1.5 ml, and 2.0 ml tubes have volume graduations and etch-marked surfaces. Three different purity classes are offered: standard, Biopur and PCR clean, depending on the application they are used for. Two more surface options help to prevent sample loss, DNA LoBind and Protein LoBind. The 0.5 ml and 1.5 ml tubes withstand g-forces of up to 30,000 x g.

Eppendorf Pipette Tips

Description Only a perfectly manufactured pipette tip can ensure that the precision of a modern pipette is maximized. epT.I.P.S. have been developed to work in perfect combination with Eppendorf pipettes. The results are reduced-force tip attachment and ejection with complete sealing. The tip refill system consists of the reusable, fully autoclavable box and the reloads refill trays. It offers the easiest possible handling in a space saving and environmentally friendly package. All components are 100% recyclable.

Eppendorf Deepwell Plates (96 & 384 wells)

Description Eppendorf Deepwell Plates with new RecoverMaxTM well designs enable high-speed pipetting, excellent mixing and high-speed centrifugation. Sophisticated color coding make reading, orientation and manual handling easier and faster. Made of virgin polypropylene, each plate type is also available in sterile quality. Eppendorf Deepwell Plate 96/500, 96/1000 and 384/200 are additionally offered as DNA LoBind and Protein LoBind. Ease of stacking, multiple sealing options as well as high chemical- and temperature-resistance make the plates excellent, multipurpose tools. The plates fulfill current guidelines: ANSI/SBS-Standards I-IV and EN ISO 9001/13485.

36

For ordering and more products see www.eppendorf.com

Photometry, PCR and Automated Pipetting

BioPhotometer plus

Description The BioPhotometer plus, a compact UV/Vis photometer, is optimized for use in molecular biology, biochemistry and cell biology labs. Preprogrammed methods for routine applications as well as automatic calculations of results guarantee safe and error-free operation. In addition to its low weight, the photometer is small, yet extremely sturdy due to a robust metal housing, which allows for easy transport and cleaning. The BioPhotometer plus is designed for use with cuvettes such as the disposable, UV transparent Eppendorf UVette®, but is also optimized for measurement of very small volumes through the use of microliter cells.

Mastercycler® pro

Description The Mastercycler pro is Eppendorf's newest PCR cycler. It offers very fast heating and cooling rates, a full gradient function and Eppendorf's new vapo.protectTM technology to reduce evaporation during PCR to a minimum. It is possible to combine up to 30 units to a network and control all units with one computer.

epMotion 5070CB

Description

The new epMotion 5070 CB increases the reproducibility of cell culture experiments. The automated pipetting system epMotion is the first automation system designed to be operated in a laminar flow hood. Automation enables more reproducible pipetting and the usage of higher density plates formats such as 96 or 384 well plates. The epMotion 5070 CB is the perfect tool for low- and mid-throughput routine cell culture work, such as seeding cells, changing media or preparing solutions with additives for cell treatment. Moreover complete assays such as cytotox, cell viability and apoptosis assays can be performed on the epMotion.

37

Fermentation & Cell Culture Services

Contract Laboratory Services

As an equipment manufacturer with in-house fermentation and cell culture laboratories, New Brunswick Scientific (NBS) offers many advantages that larger contract research organizations cannot -- low overhead and unmatched expertise in the operation and optimization of its equipment. Specializing in non-cGMP processes, NBS labs have a long history of successful scale-up and optimization of both cell culture and fermentation processes. Whether your needs are for biomass, partial purification of product or intracellular or extracellular products in raw or fractionated forms, NBS labs can help. Services include consultation for new processes, process development, biomass production, secreted protein recovery, as well as batch, fed-batch and continuous fermentation and cell culture processing. If preferred, researchers are welcome to work side-by-side with the NBS team. Please call for a free consultation.

New Brunswick Scientific's wide range of services include:

· Media formulation · Computer-control strategies · Process development · Optimization · Scale-up · Downstream purification · Benchtop to pilot-scale contract production

Expertise extends to a range of cell types including, but not limited to:

· Recombinant animal cells · NSO cells · BHK cells · CHO cells · VERO cells · 293 cells · HEK cells · Insect cells · Recombinant E. coli · Yeast with particular expertise in Pichia · Bacillus · Streptomyces · Rat liver cells · Baculovirus

Our U.S.-based facility is staffed with fermentation & cell culture specialists, and equipped with stirred-tank and packed-bed bioreactors and fermentors for research through production. For further information, contact NBS at 1.800.631.5417 or 1.732.287.1200.

Training

NBS can offer training on any of our fermentors and bioreactors. Training sessions can be set up at your site or at NBS Edison. Please call for details.

Training is designed to assist new and existing users by familiarizing them with the components and operation of their fermentor or bioreactor. Training is performed at the client site after delivery and setup of the equipment and after utility services have been connected. Training may be customized to the needs of small groups. For further information, contact NBS at 1.800.631.5417 or 1.732.287.1200.

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New Brunswick Scientific · a culture of innovation · www.nbsc.com

Selecting the Right Model Fermentor/Bioreactor

(T )T ot (W al V ol ) u W or me kin g (A Vo lu (S ) Au m I e (D P) S toc lav ) D te is ri ab Ba po liza le ct sab ble er ia le -Inl/Y Pl Pl ac ea an e st t/A /F un lg ae gi M am m al ia n/ In An se ct im al

(W) 500 mL (T) 1.3 - 14 L (W) 0.4 - 10.5 L (T) 2.5 - 14 L (W) 0.75 - 10.5 L (T) 2.5 - 14 L (W) 0.75 - 10.5 L (T) 7 - 19.5 L (W) 2.0 - 15.5 L (T) 19.5 - 40 L (W) 5.2 - 32 L (T) 19.5 - 40 L (W) 5.2 - 32 L (T) 75 - 3000 L (W) 32 - 2400 L (T) 75 - 650 L (W) 18.75 - 520 L

Model

FibraStage BioFlo 110

Ferm/Bioreactor

D A A A SIP SIP SIP SIP SIP

· · · · · · · · · · · · · ·

· · · ·

· · · · · · · · · · · · · · · ·

R or TMFC R or TMFC R or TMFC TMFC R or TMFC R or TMFC 2/4 Gas 2/3/4 Gas 2/3/4 Gas 2/3/4 Gas 2/3/4 Gas 2/3/4 Gas 2 Gas 4 Gas

BioFlo 310

Fermentor

CelliGen 310

Bioreactor

BioFlo 415

Fermentor

BioFlo 510

Fermentor

CelliGen 510

Bioreactor

·

·

BioFlo Pro

Fermentor

CelliGen Pro

Bioreactor

·

·

() Air Flow Controllers: R=Rotameter, TMFC=Thermal Mass Flow Controller (*) = Programmable Automation Controller (PAC)

A Lesson in Gas Supplementation: What's the difference between a Rotameter and a Flow Controller? Gas Supplementation

Depending upon the culture that is being grown, additional gases beyond air may be required to help sustain growth. Bacterial/yeast cultures, (Pichia, E. coli, streptomyces) often require oxygen to supplement the flow of air entering the system to meet the increased oxygen demands of these cultures. Insect cells usually require air, O2, and CO2 to sustain the dissolved oxygen and pH in the vessel at levels conducive to growth. Mammalian cells, which tend to be the most sensitive to changes in their growth environment, typically require Air, O2, N2, and CO2 to attain even tighter control of DO and pH in the medium.

Rotameters

This manually-operated device can be used to control the flow of gas or gasses entering the system. A Rotameter uses a graduated tube and a float to provide a visual indication of flow rate.

Thermal Mass Flow Controllers (TMFC)

This sophisticated device is used to enter the gas flow rate on the operator interface/display screen, and automatically control the actual gas flow rate entering your fermentor/ bioreactor vessel. Setpoint and process (current) value are displayed in SLPM (Standard Liters Per Minute).

Ve ter ss cha el n M s gea icr ble Co o nt pro ro c To lle es r so Co uch r nt scr ro ee ll n He er at In du ct io Ai n rF lo w Co Ga nt ro sM l ixi ng Va Op lid tio at ns ab Pa le ge

41 44-49

In

· · · · · · ·

50-51 54-56 58

59 59 60-61 60-62

· ·* ·* ·

R or TMFC R or TMFC

1.732.287.1200 · 1.800.631.5417 · Fax: 1.732.287.4222 · [email protected]

39

Increase Cell Culture Yields!

FibraCel® Disks: A Solid Support Growth Material for Mammalian, Animal & Insect Cells

Applications: for growth of mammalian, animal and insect cells, used predominantly for production of secreted products such as recombinant proteins & viruses. Used in NBS' FibraStage disposable Cell Culture System, Spinner Basket, CelliGen 310, CelliGen 510 and CelliGen Pro Bioreactors and is available for use with disposable bags or other bioreactor systems. Anchorage-dependent cultures: cell density & productivity can be up to ten times higher when using FibraCel disks instead of ordinary microcarriers.

FibraCel Disks

Suspension cultures: disks are electrostatically treated to facilitate suspension cells adhering to the disk and becoming entrapped in the disks' fiber system, where they remain throughout the process. Disk composition: manufactured to cGMP guidelines, composed of USP Class VI polypropylene and polyester non-woven fiber. Attachment: cells can attach within 15 - 60 minutes on FibraCel, compared to approximately six hours for cells to attach to ordinary microcarriers. FibraCel bed is inoculated in a single step -- no periodic stoppage needed to stir cells.

Advantages over standard microcarriers: · Low pressure drop across the bed for easier scale-up · High surface-to-volume ratio increases the total biomass that can be maintained in the bioreactor for higher productivity · Cells are less susceptible to shear forces from impeller blades and sparger gas bubbles as they remain entrapped in the disk bed, shielded from turbulence · Higher mass transfer of nutrients and oxygen across the disk bed results in cells that are just as viable at the bottom of the bed as they are at the top · Simple and efficient separation of biomass from secreted products. FibraCel offers sustained long-term periods of high-density growth in perfusion, without any danger of clogging and with elimination of the need for cell filtration to separate cells from the end product.

High resolution micrograph of FibraCel Disks showing polyester mesh with polypropylene support

Commonly Used Cells Successfully Grown on FibraCel Disks

FibraCel Disk Specifications

Disk Diameter Autoclavable Endotoxin Tested Bioburden Tested Cytotoxicity Tested 6 mm Yes Yes Yes Yes

Typical Quantity of FibraCel Used:

FibraStage 2.5 L Vessel 5.0 L Vessel 7.5 L Vessel 14. L Vessel

10 Grams 50 Grams 150 Grams 250 Grams 500 Grams

Hybridoma DA4.4, 123A 127A, GAMMA, 67-9-B Anchorage-Dependent 3T3, COS, Human Osteosarcoma, MRC-5, BHK, VERO, CHO, rCHO-tPA, rCHO- Hep B Surface Antigen, HEK 293, rHEK 293, rC127 - Hep Surface Antigen, Normal Human Fibroblasts, Stroma, Hepatocytes Insect Tn-368 SF9 rSF9 Hi-5

Ordering Information

Catalog No. M1292-9984 M1292-9988 Description FibraCel Disks, 50 grams FibraCel Disks, 250 grams

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New Brunswick Scientific · a culture of innovation · www.nbsc.com

Cell Culture Systems

FibraStageTM Disposable, High-Yield Cell Culture System

A small scale disposable system simplifies production of secreted products, virus or cells from anchorage-dependent and some suspension cultures. Disposables: Pre-sterliized, single-use 500 mL bottle(s), pre-filled with 10 grams of the FibraCel® disks provide a low-shear environment and extremely high-surface area for cell growth. NonDisposable Components: FibraStage console with adjustable speed platform collapses/expands the bottles, exposing cells to nutrients and gasses; and a controller. Requires use of CO2 incubator for temperature and gas control. Control & Display: Keypad with LED display regulates bottle movement and "hold time", for efficient nutrient and gas exchange. Capacity: 500 mL bottles; Console holds up to four bottles. Overall dimensions: 10.4" W x 14.1" D x 12.2" H (26.4 x 35.9 x 30.9 cm). Ask about our 30-day in-lab trial offer.

Model Catalog No. M1327-1000 M1327-9980 Description Console & Controller* Disposable Bottles, Pack of 4

FibraStage

FibraStageTM Disposable, High-Yield Continuous Cell Culture System

The FibraStage Continuous System includes everything listed above plus a pump module to provide continuous media exchange in the FibraStage continuous bottles. Each bottle is equipped with additional inlet and outlet lines for media re-circulation that links to an optional media addition bottle. This provides continuous exchange of nutrients, eliminating the labor for media exchange and avoiding possible contamination during media exchange. Accommodates up to 3 bottles.

Model Catalog No. M1327-1001 M1327-9981 Description Console & Controller* Disposable Bottles, Pack of 4

FibraStage Continuous System

FibraStage Accessories

Catalog No. Description Additional Parts for FibraStage Systems: P0620-6460 FibraStage Adapter (required for Europe) P0640-0518 FibraStage Bottle Opener (To open bottle for harvesting disks) P0440-0500 Glucose Monitoring Kit (Includes 50 test strips) P0440-0508 Glucose Test Strips (Includes 50 test strips) P0620-6470 9-Pin Signal Cable P0640-0610 Crystal Violet dye (100 mL for cell counting - Nucleus counting kit) P0640-0600 Cell Dissociation Tool (To compress the bottom of the bottle) P0640-0620 FibraStage Bottle Cap For Continuous System Only: P0620-3040 FibraStage 3-Head Pump (3 individually controlled motor units) tubing & pump head not included. P0740-2730 Tubing Set & Pump Head (Includes 1 set of tubing, tubing connectors and pump head) P0740-2740 Tubing Only (Includes 5 sets of tubing) M1228-5014 2L Media Addition Bottle * Includes forceps for cell sampling and crystal violet dye.

1.732.287.1200 · 1.800.631.5417 · Fax: 1.732.287.4222 · [email protected]

41

Automated Cell Counting Systems

NucleoCounter® Automated Mammalian Cell Counter

Available in North America only. Designed to automatically count mammalian cells in research and production environments. An integrated fluorescence microscope in the NucleoCounter detects signals from a fluorescent dye, propidium iodide, that is bound to the cells' nuclei. The resulting counts can be total cells or viable cells depending on sample preparation. Each count requires one NucleoCassette® and aliquots of Reagent A100 and Reagent B. Overall Dimensions:15" L x 8.7" D x 10.2" H (22 x 38 x 26 cm). Net weight: 7 lbs (3 kg). Requires 120 VAC.

Model Catalog No. Electrical Service 120V, 60Hz

Compact NucleoCounter & NucleoCounter YC-100 need no maintenance, calibration or cleaning

NucleoCounter

M1293-0000

Required Accessories (see additional accessories below) P0820-5190 P0820-5180 P0820-5200 M1293-0020 Reagent A100 (Lysis Buffer). Reagent B (Stabilization Buffer). Reagent C (Blood Applications) Starter Kit - Includes one bottle of reagent A100 and one bottle of Reagent B, 2 dispensers, reagent holder, 100 NucleoCassettes & NucleoView software

NucleoCounter® YC-100 Automated Yeast Cell Counter

Available in North America only. Designed to automatically count yeast cells in R&D and QC production environments, brewery & biofuels research. Suitable for performing total yeast cell counts as well as estimating viability. An integrated fluorescence microscope in the NucleoCounter detects signals from a fluorescent dye, propidium iodide, that is bound to the cells' nuclei. Suitable for performing total yeast cell counts well as estimating viability. Can be connected to a PC for documentation, data storage and image collection. Each count requires one NucleoCassetteTM and aliquots of Reagent Y100. Overall Dimensions: 15" L x 8.7" D x 10.2" H (22 x 38 x 26 cm). Net weight: 7 lbs (3 kg). Requires 120 VAC.

Model Catalog No. Electrical Service 120V, 60Hz

NucleoView Software automatically calculates cell viability, allows you to view the sample image, and download data to your PC

NucleoCounter YC-100

M1293-1000

Required Accessories (see additional accessories below) P0820-5185 Reagent YC-100

Common NucleoCounter Accessories

Catalog No. M1293-0100 Description NucleoCassettes® ­ Disposable, plastic sampling iodide, used to count cells when filled with treated sample and inserted into the NucleoCounter. Plunger loading ensures that the correct amount of sample (50 µl) is taken into the cassette with 2 µl delivered to the counting chamber. Cassettes are packaged in foil bags of 10. Sold in quantities of 100 cassettes. QC Test Kit Dispenser Pump ­ Used to dispense microliter quantities of Reagent A100 and Reagent B Reagent Holder holds two 500 mL Reagent Bottles NucleoView® Software ­ Enables the user to view the counted cell area and data log the count for future use.

M1293-0900 P0820-5179 P0860-5178 P0540-5440

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Growing CHO Cells

This paper is one in a series of guides to growing cultures in NBS fermentors and bioreactors. For our other papers on growing mammalian, insect, bacterial and yeast cultures, please see our website: www.nbsc.com in the Technical Info section.

Chinese Hamster Ovary cells, or CHO cells, are commonly used in biotechnology for protein production in the growing sector of mammalian cell culture. Mammalian cell culture has become increasingly popular due to the ability of Eukaryotic cells to achieve more complex post-translational modification of proteins.

pH/DO Control: pH control in animal cell cultures is typically accomplished through a combination of liquid and gas additions. CO2 is usually sparged into the media as a means of lowering the pH and addition of an alkaline solution, typically sodium bicarbonate solution, is often made to increase the pH. pH deadbands of 0.1-0.2 may also be used. These will reduce the volume of base being added. DO control: DO control is manually or automatically accomplished by adding various gasses into the media to either raise or lower the DO. Air supplementation is most commonly used to maintain and raise DO. O2 is sometimes supplemented if air is not capable of control alone. N2 is used to reduce the DO level of the media. Impeller Choices: Many mammalian cell lines, and CHO in particular, can be grown using a wide variety of different impellers. Pitched blade and Marine impellers are popular when growing suspension and anchorage dependent cells in batch, fed-batch, or perfusion. By design, these impellers deliver excellent mixing while producing minimal shear. Filter or screen impellers such as our Spin Filter and Cell Lift® are useful when growing cultures where cell-free media needs to be removed, as either harvest or waste, while cells are retained within the media. Our Packed Bed Basket impellers use FibraCel® Disks as the cell attachment matrix and can produce very high cell densities as cells grow tightly in or on the fibrous bed material. This method is most commonly used when secreted proteins or virus are the desired product. (For more information on impellers, see page 52).

Medium: Many pre-defined media are available for mammalian CHO cultures from companies such as Hyclone, GIBCO, JRH, and Sigma. While options exist for media containing serum, the use of animal component free chemically defined media is quickly becoming the industry standard.

Batch/Fed-Batch/Continuous: There are three ways which CHO cells are commonly grown. In a batch method, all the necessary media and nutrients for the run are added to the vessel after sterilization and the vessel is inoculated by adding cells. The cells are allowed to grow until there is no more available carbon/nitrogen source and they begin to die. At this point the cells would either be transferred or harvested. Fed-batch starts out similar to a batch method, but the vessel is only partially filled with media and nutrients leaving room to add additional components. When the cells have consumed all available carbon/nitrogen sources they are fed a supplement and they continue to grow. This is sometimes repeated several times. In a continuous system cells are allowed to grow up to a certain density at which point media is removed and an equal amount of fresh media is added. Continuous systems can often operate for very long runs, sometimes over a month.

Typical Cell Densities: Typical Process Control Setpoints For CHO: Culture Type Temperature........................................................................37°C pH .........................................................................................7.2 Dissolved Oxygen ...........................................................35-50% Agitation ................................................................70-130 rpm* Gas Control .......................................................................4-Gas Inoculum ..............................................................1.0 - 4.0 x 105 *Agitation ranges will vary depending on the vessel size and the type of impeller used. Packed-Bed FibraCel® Disks Setpoints for CHO should be entered prior to inoculation, and, except for DO which often remains high, the media should be allowed to equilibrate prior to inoculation. DO remains high because it takes time for the O2 to permeate out of the media as it is not yet being utilized by the culture. An initial DO value of approx. 100% air saturation is acceptable and will decrease as the cells metabolize it. Setpoints are commonly controlled in auto mode. Process Average cell density (cells/ml) 2 - 4 x 106 3 - 6 x 106 10 - 15 x 106 2 - 3 x 106 10 - 15 x 106

Suspension

Batch Fed-Batch Perfusion Batch Perfusion

Microcarrier

Perfusion

0.5 - 1 x 108

1.732.287.1200 · 1.800.631.5417 · Fax: 1.732.287.4222 · [email protected]

43

Fermentation & Cell Culture Systems

BioFlo® 110 Modular Benchtop Fermentor/Bioreactor, 1.3 - 14 Liters

BioFlo 110 is an easy-to-use, cost-effective and convenient modular microbial fermentor and/or cell culture bioreactor system. Packaged in kits to simplify ordering, individual components may also be purchased at any time, to meet changing needs. Features a multi-language PCU, and choice of interchangeable autoclavable vessels, 1.3 to 14 L (total volume).

1 2 12 13 14 15 3 4 5 6

11

10 9

8

7

BioFlo 110 System Controllers And Components

1. PCU (Primary Control Unit) - an easy to use 32-loop controller with bright display, supports from one to four vessels. It provides the interface for all control functions. 2. Levels/Foam Controller - accepts up to three conduction probes for automatic control of antifoam, addition and media addition/removal. 3. pH/dO2 Controller - supports one pH and one dissolved oxygen probe. Requires acid base addition pumps and/or the four gas controller for pH control. 4. Power Controller - supports temperature and agitation control in one vessel, and also has 5 controlled power outlets for liquid addition pumps. 5. Thermal Mass Flow Controller (TMFC) - TMFC replaces the rotameter to provide automated gas flow control. Advantages include a digital display of the gas flow rate on the PCU; with the ability to log gas flow data using an optional data acquisition package, such a BioCommand® Lite or BioCommand Plus. 6. Gas Mix Controller - four valves allow automatic rationing of two, three, or four gasses (air, oxygen, carbon dioxide and nitrogen) for gentle regulation of pH and dO2 in cell culture, and for oxygen enhancement in fermentation. Works together with the pH/dO2 controller. 7. Four-Pump Module - four independent PWM peristaltic pumps utilize Watson Marlow 101 pump heads. Use for acid and base, antifoam, nutrient additions, and perfusion. One four-pump module may be shared by multiple vessels. Vessel Stand - used with a heat-blanketed vessel. Heat Blanket (HB) - wraps around vessels, adding heat when required to maintain vessel contents at temperature setpoint. The blanket has two large windows for viewing. Sterile Sampler - a syringe-activated device for removing media samples without contaminating the culture. The sampler installs in a headplate port. Samplers are sized to vessels. Direct Drive Shaft - available in fermentation & cell culture vessels. Exhaust Condenser - essential in most applications for keeping exhaust filters dry and vessel contents from evaporating during culture. Magnetic Drive Shaft - only available in cell culture vessel. Addition Bottles - for acid, base and additions; can be mounted directly to vessel stand. Jacket Water Heater - used with water jacket vessel. The heater controls jacket water heater temperature to maintain vessel's temperature setpoint.

8. 9.

10.

11. 12.

13. 14. 15.

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BioFlo® 110 Components & Vessel Accessories

Septum - installs in a 12mm headplate port to enable additions by syringe, such as inoculum. Baffle Assembly - a removable assembly of flat stainlesssteel panels that protrude from the fermentor vessel wall to createturbulence in the media, improving mixing and aeration ofvessel contents. Baffles are not used with shear-sensitivemammalian or insect cells. Foam Trap - this liquid trap installs between the exhaust condenser and filter to catch liquid that inadvertently passes through the condenser. Tri-port Adapter - a three-addition fitting that requires only one 12 mm headplate port. Angled Autoclave Rack - reduces the autoclave chamber height requirement by cradling 7.5 & 14 L vessels off the vertical. Probe Adapter - retains 12 mm probes with a headplatemounted compression fitting that won't "bake" onto the probe surface. Allows continuous penetration-depth adjustment. Sparge Ring - a headplate mounted air-inlet tube. The immersed end forms a perforated ring that sits directly beneath the impeller blade tips for efficient oxygenation. pH/dO2 Controller Kit - includes one pH/dO2 controller, pH & dO2 probes sized to the vessel, adapters and cables.

How To Order BioFlo 110 Kits Fermentor:

1. Determine your requirements: · Select an Advanced or Basic Fermentor (Advanced kits include a more complete array of controls and accessories. See Kit Contents on page 42) · For Advanced Fermentors, choose temperature control using heat blanket with cooling coil, or water jacket with heater · Select Vessel Size & Voltage 2. Order the Part Number corresponding to your choices from the tables on page 43

Bioreactor:

1. Determine your requirements: · Agitation Drive: magnetic-coupled or direct-coupled · Temperature Control using heat blanket with cooling coil, or water-jacket · Vessel Size & Voltage · Impellers: spin filter with marine blade, marine blade, or pitched blade 2. Order the Part Number corresponding to your choices from the tables on page 44

Optional

3. Add a Gas-Mix Controller to Advanced and Basic Fermentors if you want the ability to blend oxygen with the sparge air. 4. Add a Thermal Mass Flow Controller if you want automated control and digital display of the gas flow rate on your PCU. 5. Order Up to Three Additional Vessels for use with the Primary Control Unit included with your fermentor. Choose from the Add-A-Vessel Kits on page 43. (Kit contents listed on page 42) 6. For each additional vessel, order one Power Control Unit (for temperature, agitation and pump power outlets), plus Controllers & pH/D.O. Controller Kits as desired on page 44.

Optional

3. Add a Thermal Mass Flow Controller if you want automated control and digital display of the gas flow rate on your PCU. 4. To run more than one vessel from the Primary controller included with your bioreactor, choose from the Add-A-Vessel Kits on page 44. (Kit contents listed on page 42) 5. For each additional vessel, order one Power Control Unit (for temperature, agitation and pump power outlets), plus Controllers & pH/D.O. Controller Kits as desired on page 44.

How To Order BioFlo 110 Individual Components

You can order components to assemble a custom fermentor or bioreactor. · Determine your requirements · Order Add-a-Vessel Kits from pages 42 - 43 · Order controllers from page 44 · Order accessories from pages 45 · Order pH and dO2 probes and cables from pages 66 - 70. · Be sure vessel accessories and probes match vessel size

NOTE: Cell culture and fermentor vessels can run simultaneously from the same PCU

1.732.287.1200 · 1.800.631.5417 · Fax: 1.732.287.4222 · [email protected]

45

Fermentation & Cell Culture Systems

Kit Contents for Fermentation and Cell Culture - BioFlo® 110

KIT CONTENTS

Vessel Kit - BASIC* Vessel Kit - ADVANCED* Primary Control Unit (supports 4 vessels) Power Controller (Temperature and Agitation) Foam/Levels Controller Foam Probe, Adapter and Cable pH / dO2 Controller pH Probe, Adapter and Cable dO2 Probe, Adapter and Cable 4-Pumps Module Gas Mix Controller Thermal Mass Flow Controller (Optional)

(*) For details of vessel kit components, see Add-A-Vessel table below

ADVANCED ADVANCED FERMENTATION KIT CELL CULTURE KIT

BASIC FERMENTATION KIT

· · · · · · · · ·

· · · · · · · · · ·

· · ·

Add-A-Vessel Kits for Fermentation and Cell Culture - BioFlo 110

BioFlo 110 Add-A-Vessel Kits contain ancillary components required for independent operation of a 2nd, 3rd or 4th vessel. Additional control components are required. Basic and Advanced kits differ in their included accessories.

COMPONENT

VESSEL KIT - ADVANCED VESSEL KIT - ADVANCED CELL CULTURE FERMENTATION

Heat Blanket (HB) Water Jacket (WJ) Heat Blanket (HB) Water Jacket (WJ)

VESSEL KIT - BASIC FERMENTATION

Heat Blanket Water Jacket (HB) (WJ)

Dish-Bottom Vessel w/Stainless Steel Headplate Vessel Stand Agitation Motor, 50-1200 rpm Agitation Motor, 30-300 rpm Direct Drive Assembly Magnetic Drive Assembly Option Heater Blanket Jacket Water Heater Immersion Cooling Coil Cooling Water Valve Thermowell RTD Probe Rotameter Baffle Assembly Two Rushton Impellers One Pitched Blade Impeller Exhaust Condenser Sterile Sampler Tri-port Adapter Septum Kit Liquid addition Tube and Headplate Adapter Three Addition Bottles and Tubing

· · · · · · · · · · · · · · · · · ·

· · · · · · · · · · · · · · · ·

Need help?

· · · · · · · · · · · · · · · · · ·

· · · · · · · · · · · · · · · ·

· · · · · · · · · · · ·

· · · · · · · · · ·

BioFlo 110 Protocols for commonly used cell lines are free for download at www.nbsc.com/bf110.aspx

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Advanced Fermentation Kits - BioFlo® 110

HB kits include Vessel, Heat Blanket (HB) & Immersed Cooling Coil. WJ kits include Water-Jacketed (WJ) Vessel and Jacket Water Heater Total Volume (L) Working Volume (L) Electrical Service HB Catalog No. WJ Catalog No.

1.3 3.0 7.5 14.0

0.4 - 1.0 0.8 - 2.2 2.0 - 5.6 4.0 - 10.5

100 - 120 V 200 - 240 V 100 - 120 V 200 - 240 V 100 - 120 V 200 - 240 V 100 - 120 V 200 - 240 V

M1273-1121 M1273-1151 M1273-1122 M1273-1152 M1273-1125 M1273-1155 M1273-1130 M1273-1150

M1273-1111 M1273-1161 M1273-1112 M1273-1162 M1273-1115 M1273-1165 M1273-1120 M1273-1160

ADD-A-VESSEL KITS - ADVANCED FERMENTATION Total Volume (L) Working Volume (L) HB Catalog No. WJ Catalog No.

1.3 3.0 7.5 14.0

0.4 - 1.0 0.8 - 2.2 2.0 - 5.6 4.0 - 10.5

M1273-1601 M1273-1602 M1273-1605 M1273-1610

M1273-1611 M1273-1612 M1273-1615 M1273-1620

() When ordering Add-a-Vessel Kits, ancillary controllers are required. See page 44.

Basic Fermentation Kits - BioFlo 110

HB kits include a vessel, Heat Blanket with PID temperature and agitation control, plus manual air flow control. Add additional control capabilities or vessels at any time. Additional vessels are available with heat blanket or water jacket. See chart below Total Volume (L) Working Volume (L) Electrical Service HB Catalog No. WJ Catalog No.

1.3 3.0 7.5 14.0

0.4 - 1.0 0.8 - 2.2 2.0 - 5.6 4.0 - 10.5

100 - 120 V 200 - 240 V 100 - 120 V 200 - 240 V 100 - 120 V 200 - 240 V 100 - 120 V 200 - 240 V

M1273-1101 M1273-1141 M1273-1102 M1273-1142 M1273-1105 M1273-1145 M1273-1110 M1273-1140

M1273-1621 M1273-1631 M1273-1622 M1273-1632 M1273-1625 M1273-1635 M1273-1630 M1273-1640

ADD-A-VESSEL KITS - BASIC FERMENTATION Total Volume (L) Working Volume (L) HB Catalog No. WJ Catalog No.

1.3 3.0 7.5 14.0

0.4 - 1.0 0.8 - 2.2 2.0 - 5.6 4.0 - 10.5

M1273-1001 M1273-1002 M1273-1005 M1273-1010

M1273-1011 M1273-1012 M1273-1015 M1273-1020

() When ordering Add-a-Vessel kits, ancillary controllers are required. See page 44.

1.732.287.1200 · 1.800.631.5417 · Fax: 1.732.287.4222 · [email protected]

47

Fermentation & Cell Culture Systems

Advanced Cell Culture Kits - BioFlo® 110

NBS offers Advanced Cell Culture Kits with water-jacket vessel or heat blanket vessels. Each is offered with a choice of magnetic (M) or direct-drive (D) agitation systems. Total Volume (L) Working Volume (L) Electrical Service Drive* HB Catalog No. WJ Catalog No.

100-120 V 1.3 0.4 - 1.0 200-240 V 100-120 V 3.0 0.8 - 2.2 200-240 V 100-120 V 7.5 2.0 - 5.6 200-240 V 100-120 V 14.0 4.0 - 10.5 200-240 V

(*) "M" or "D" indicates Magnetic drive or Direct drive assembly and motor

M D M D M D M D M D M D M D M D

M1273-1201 M1273-1301 M1273-1401 M1273-1501 M1273-1202 M1273-1302 M1273-1402 M1273-1502 M1273-1205 M1273-1305 M1273-1405 M1273-1505 M1273-1210 M1273-1310 M1273-1410 M1273-1510

M1273-1211 M1273-1311 M1273-1171 M1273-1371 M1273-1212 M1273-1312 M1273-1172 M1273-1372 M1273-1215 M1273-1315 M1273-1175 M1273-1375 M1273-1220 M1273-1320 M1273-1170 M1273-1370

ADD-A-VESSEL KITS - ADVANCED CELL CULTURE Total Volume (L) Working Volume (L) Agitation Drive HB Catalog No. WJ Catalog No.

1.3 3.0 7.5 14.0 1.3 3.0 7.5 14.0

0.4 - 1.0 0.8 - 2.2 2.0 - 5.6 4.0 - 10.5 0.4 - 1.0 0.8 - 2.2 2.0 - 5.6 4.0 - 10.5

Direct (D)

M1273-1041 M1273-1042 M1273-1045 M1273-1050 M1273-1051 M1273-1052 M1273-1055 M1273-1060

M1273-1021 M1273-1022 M1273-1025 M1273-1030 M1273-1031 M1273-1032 M1273-1035 M1273-1040

Magnetic (M)

() When ordering Add-a-Vessel Kits, ancillary controllers are required. See below.

Control Components For Fermentation & Cell Culture - BioFlo® 110

Catalog No. Controllers M1273-3101 M1273-3100 M1273-3110 M1273-3103 M1273-3106 M1273-3116 M1273-3104 M1273-3102 M1273-3109 M1273-3112 Description Primary Control Unit Power Controller, 100-120V Power Controller, 200-240V Levels/Foam Controller (no probes, cables or adapters) 4-Pump Module, 100-120V 4-Pump Module, 200-240V Gas Mix Controller pH/dO2 Controller (no probes, cables or adapters) Thermal Mass Flow Controller (0 - 20 SLPM) for fermentation Thermal Mass Flow Controller (0 - 5 SLPM) for cell culture Catalog No. Heater Blankets M1273-9931 M1273-9932 M1273-9930 M1273-9933 Description For 1.3 L Vessels For 3.0 L Vessels For 7.5 L Vessels For 14 L Vessels

BIOFLO 110 PROBE KITS - Suitable for fermentation and cell culture applications using heat-blanketed or water-jacketed vessels pH Probe Kits M1273-9970 1.3 L Direct or Magnetic Drive M1273-9977 3 L Direct or Magnetic Drive M1273-9982 7.5 L Direct or Magnetic Drive M1273-9985 14 L Direct or Magnetic Drive dO2 Probe Kits M1273-9974 M1273-9975 M1273-9979 M1273-9986 M1273-9988 Motors M1273-3120 M1273-3125 M1273-3135 M1273-3130

pH/dO2 Controller Kits (includes controller, probes, cables & adapters) M1273-9850 1.3 L Total Vessel Volume M1273-9851 3.0 L Total Vessel Volume M1273-9852 7.5 L Total Vessel Volume M1273-9853 14 L Total Vessel Volume Jacket Water Heaters M1273-3107 Jacket Water Heater, 1.3 - 3 L Vessels M1273-3108 Same as above, 7.5 - 14 L Vessels

1.3 L Direct Drive 1.3 L Magnetic Drive 3 L Direct or Magnetic Drive 7.5 L Direct or Magnetic Drive 14 L Direct or Magnetic Drive Direct Drive , Ferm., 1.3 - 3.0 L Direct Drive, Ferm., 7.5 - 14.0 L Direct Drive, Cell Culture, all vessels Magnetic Drive, all vessels

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Other Accessories - BioFlo® 110

Catalog No. Description Catalog No. Description Foam And Level Probe Kits (includes probe, cable & adapter) M1273-9947 Foam Level Probe Kit, for 1.3 L Vessels M1273-9951 Foam Level Probe Kit, 3 L Vessels M1273-9960 Foam Level Probe Kit, 7.5 & 14 L Vessels M1273-9942 Foam Level Probe Kit, 250 mm Exhaust Condensers M1273-9945 Exhaust Condenser, 1.3, 3 & 7.5 L Vessels M1273-9957 For 14 L Vessels Headplate Adapters & Plugs M1273-9961 Tri-Port Adapter M1273-5054 6 mm Adapter Kit, 6 mm port to 6 mm tube M1273-5056 Adapter Kit, 12 mm port to 6 mm tube with set screws M1273-5058 Adapter Kit, 12 mm port to 12 mm tube, Exhaust with set screws Condenser M1273-5040 Probe Adapter, 12 mm, compression M1273-5042 Level/Sample Adapter, 6 mm, compression M1273-9575 Addition Tube for 6 mm adapter M1273-9405 Plug, 6.35 mm port M1273-9406 Plug, 12 mm port M1273-9407 Plug, 19 mm port M1273-9900 Headplate-Port Washers & O-rings M1273-3031 Septum Kit for 12 mm port Impellers M1273-9291 6-Bladed Fermentation Impeller for 1.3 & 3 L Vessels M1273-9292 6-Bladed Impeller, 7.5 L Vessels M1273-9293 6-Bladed Impeller, 14 L Vessels M1273-9206 Impeller, Pitched-blade (standard upflow) for 1.3 and 3 L Vessels Rushton M1273-9207 Impeller, Pitched-blade (standard upflow) Impeller for 7.5 and 14 L Vessels M1273-9290 Impeller, Pitched-blade (downflow) for 1.3 & 3 L Vessels M1230-9212 Impeller, Pitched-blade (downflow) for 7.5 & 14 L Vessels Pitched M1273-9901 Marine Blade Impeller Kit, 1.3 & 3.0 L Blade M1273-9902 Marine Blade Impeller Kit, 7.5 & 14 L Impeller Spin Filters M1273-3201 Spin Filter, Suspension Cells, 1.3 L M1273-3202 Spin Filter, Suspension Cells, 3 L Vessels M1273-3205 Spin Filter, Suspension Cells, 7.5 L Vessels M1273-3210 Spin Filter, Suspension Cells, 14 L Vessels M1273-3211 Spin Filter, Microcarriers, 1.3 L M1273-3212 Spin Filter, Microcarriers, 3 L Vessels M1273-3215 Spin Filter, Microcarriers, 7.5 L Vessels M1273-3220 Spin Filter, Microcarriers, 14 L Vessels Microspargers Spin Filter M1273-5007 Sintered/Porous Microsparger, 1.3 L, HB with M1273-5003 Sintered/Porous Microsparger,1.3 L WJ Marine M1273-5004 Sintered/Porous Microsparger, 3 L vessel Blade M1273-5005 Sintered/Porous Microsparger, 7.5 L vessel M1273-5006 Sintered/Porous Microsparger, 14 L vessel NON-JACKETED VESSELS

VESSEL

Replacement Vessels M1273-9907 Replacement Heat Blanket Vessel (glass only), 1.3 L M1273-9909 Replacement Vessel, as above 3 L M1273-9916 Replacement Vessel, as above 7.5 L M1273-9918 Replacement Vessel, as above 14 L M1273-9908 Replacement Water-Jacketed Vessel (glass only), 1.3 L M1273-9915 Replacement Vessel, as above, 3 L M1273-9917 Replacement Vessel, as above, 7.5 L M1273-9919 Replacement Vessel, as above, 14 L Rotameters M1273-5063 Rotameter Assembly, 0.5 LPM M1273-5062 Rotameter Assembly, 1.2 LPM M1273-5073 Rotameter Assembly, 2.5 LPM M1273-5064 Rotameter Assembly, 5 LPM M1273-5074 Rotameter Assembly, 11.4 LPM M1273-5065 Rotameter Assembly, 20 LPM Sampling & Inoculation M1273-9946 Sterile Sampling Kit, 1.3 L Vessels M1273-9949 Sterile Sampling Kit, 3 L Vessels M1273-9953 Sterile Sampling Kit, 7.5 L Vessels M1273-9956 Sterile Sampling Kit, 14 L Vessels Utilities, Autoclave Accessories M1273-9266 Angled Autoclave Rack for 7.5 L & 14 L Vessels M1273-5001 Water Regulator/Filter Kit (4 Manifolds) Ability to add 1 to 4 vessels M1117-2040 Water Regulator/Filter Kit M1273-5002 Air Regulator/Filter Kit (4 Manifolds) Ability to add 1 to 4 vessels M1230-3030 Air Regulator/Filter Kit M1273-9936 Bearing Housing Cap, 10 Pack Miscellaneous M1273-9989 Addition Bottle Kit, 250 mL M1273-9990 Addition Bottle Kit, 500 mL M1273-9940 Addition Bottle Holder Kt M1273-5071 1-Pump Masterflex EZ Load, 110V M1273-5070 1-Pump Masterflex EZ Load, 220V M1273-9991 Spare Parts Kit, 1.3 - 3 L, HB M1273-9992 Spare Parts Kit, 7.5 - 14.5 L, HB M1273-9998 Spare Parts Kit, 1.3 - 3 L, WJ M1273-9999 Spare Parts Kit, 7.5. - 14 L, WJ P0860-1050 Silicone Grease for o-rings/seals P0160-4460 Tubing Clamp P0240-2680 Female, Polysulfone Quick Connect, 1/4" P0240-2670 Male, Polysulfone Quick Connect, 1/4" See also automated data logging & control accessories pages 63 - 65

Glass Replacement Vessels

Sampling Kit

Angled Autoclave Rack

Addition Bottle

Approximate Vessel Dimensions, as Prepared for Autoclave

WATER-JACKETED VESSELS

VESSEL

Total Volume (L)

With Exhaust Condenser

Without Exhaust Condenser

Length

Inches cm

Width 8.5 22 11.5 29 11.5 29

Height 22 25.5 29 56 65 74

Diameter

Inches cm

Height

Inches cm

Inches cm Inches cm

Total Volume (L)

With Exhaust Condenser

Without Exhaust Condenser

Length

Inches cm

Width 11.5 11.5 11.5 11.5 29 29 29 29

Height 20.5 22.5 26.8 31.5 52 56 68 80

Diameter

Inches cm

Height

Inches cm

Inches cm Inches cm

1.3 & 3.0 9.5 7.5 14.5 14.0 11.5

24 37 29

8.5 11.5 11.5

22 29 29

16.5 42 19.5 49.5 24 61

On Optional Slant Rack 7.5 14.0 28 31.5 71 80 11.5 29 11.5 29 18 20 46 51 -- -- -- -- -- -- -- --

1.3 3.0 7.5 14.0

11.5 11.5 11.5 11.5

29 29 29 29

9.25 9.25 11.5 11.5

24 24 29 29

16 18 20.5 26.5

41 45 52 67

On Optional Slant Rack 7.5 14.0 26 28.5 66 72 11.5 29 11.5 29 18.5 47 20.5 52 -- -- -- -- -- -- -- --

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49

Fermentation & Cell Culture Systems

cGMP nt omplia c

BioFlo® 310 Compact Autoclavable Benchtop Fermentor, 2.5 - 14 Liters

BioFlo 310 is a versatile benchtop fermentor with an advanced controller and touchscreen interface capable of operating up to four reactors simultaneously. This compact system can operate in batch, fed batch or continuous mode and is suitable for growth of bacteria, yeast, fungi and plant cells. Sophisticated Master Control Station: Runs on the powerful RPC (Reactor Process Controller) which can control up to 4 vessels, 32 control loops per reactor, 2 USB ports plus everything that is included in the utility station described below. Stores 10 recipes & trends up to 8 parameters per reactor. (See page 53). Adjustable-position, 15" industrial color Touchscreen Display: is standard with the first fermentor. Not required for 2nd, 3rd, and 4th reactor. Compact Utility Station: Each includes 3 built-in pumps, control of standard parameters (Agitation, Temperature, pH, DO, Foam/Level, plus Gas Loops depending on the gas selection). Up to 7 analog inputs and 7 analog outputs, 1 TMFC* (0.4 - 20 SLPM**) with 4 solenoid valves and much more. Options: include up to 4 TMFCs with choice of flow rates, 2nd pH - DO/Redox and 8-port serial box for scales. Vessel Sizes: Choice of interchangeable 2.5, 5, 7.5 & 14 L vessels. Vessel Assembly: includes glass vessel with stainless-steel dished jacketed bottom, direct drive, motor, exhaust condenser, sampling assembly, two 6-blade Rushton-type impellers, one foam/level sensor, and much more. Sensor Kits: pH/DO kits include probe and cable. Redox kit is also available. Basic and advanced validation packages are available. Pre-configured kits are available to simplify ordering.

Trend up to 144 hours of process data. Monitor and control it all on a large, 15" touchscreen interface

Pre-Configured Kits

Catalog No.

Total Volume (L)

Working Volume (L)

Electrical Service

System Includes Includes BioFlo 310 Master Control Station, 1 TMFC (0.4 - 20 SLPM) with 4-Gas Mixing, Touchscreen Display, Vessel Assembly and a pH/DO Probe Kit.

M1287-1170 M1287-1171 M1287-1172 M1287-1173 M1287-1174 M1287-1175 M1287-1176 M1287-1177

2.5 5.0 7.5 14.0 2.5 5.0 7.5 14.0

0.75 - 1.75 1.25 - 3.75 2.0 - 5.5 3.0 - 10.5 0.75 - 1.75 1.25 - 3.75 2.0 - 5.5 3.0 - 10.5

100 - 120V, 50/60 Hz 100 - 120V, 50/60 Hz 100 - 120V, 50/60 Hz 100 - 120V, 50/60 Hz 200 - 240V, 50/60 Hz 200 - 240V, 50/60 Hz 200 - 240V, 50/60 Hz 200 - 240V, 50/60 Hz

How to order a BioFlo 310 with multiple Thermal Mass Flow Controllers (TMFCs) with various ranges, and/or 2nd, 3rd and 4th Utility Station, and/or 2nd DO - pH/Redox sensors

1. Choose voltage: · 100 - 120V . . . . . . . . . . . . . . . . . . . . . . . . . M1287-1010 · 200 - 240V . . . . . . . . . . . . . . . . . . . . . . . . . M1287-1020 2. Choose CABINET: (see descriptions above) · Master Control Station . . . . . . . . . . . . . . . M1287-2110 · Utility Station with no display. . . . . . . . . . . M1287-2112 3. Choose GASES: · 0 TMFC (requires a Rotameter)*** . . . . . . . M1287-2010 · 1 Rotameter (0-20 SLPM) . . . . . . . . . . . M1287-3510 · 1 Rotameter (0-5 SLPM) . . . . . . . . . . . . M1287-3520 · 1 TMFC (0.4-20 SLPM)*** . . . . . . . . . . . . . M1287-2012 · 1 TMFC (0.1-5 SLPM)*** . . . . . . . . . . . . . . M1287-2011 · 2 TMFC (0.1-5 SLPM) . . . . . . . . . . . . . . . . . M1287-2013 · 2 TMFC (0.4-20 SLPM) . . . . . . . . . . . . . . . . M1287-2014 · 3 TMFC (0.4-20 SLPM) . . . . . . . . . . . . . . . . M1287-2016 · 4 TMFC (0.1-5 SLPM) . . . . . . . . . . . . . . . . . M1287-2017 · 4 TMFC (0.4-20 SLPM) . . . . . . . . . . . . . . . . M1287-2018 3a. When should you select multiple TMFCs? When you require each gas to be individually contolled, monitored and displayed in SLPM. 3b. Which TMFC is right for your process? For fermentation we recommend 0.4 - 20 SLPM and 0.1 - 5 SLPM for smaller size vessels or multiple TMFCs. 4. Vessel Kits: Size Total Volume (L) ADD-A-VESSEL KITS With Motor Catalog No. Without Motor Catalog No.

2.5 5.0 7.5 14.0

M1287-0300 M1287-0301 M1287-0302 M1287-0303

M1287-0320 M1287-0321 M1287-0322 M1287-0323

5. 2nd DO - pH/Redox board: Factory installed . . . . . . . . . . . . . . . . . . . . M1287-3540 6. Add Probe Kits: pH/DO Probe Kits M1287-0400 M1287-0401 M1287-0402 M1287-0403 . . .2.5 L . . .5.0 L . . .7.5 L . . .14 L

Redox Probe Kits M1287-0410 M1287-0411 (*) TMFC = Thermal Mass Flow Controller (**) SLPM = Standard Liters Per Minute M1287-0412 (***) 0 and 1 TMFCs are built with 4-gas manifold for 4 gas mixing M1287-0413 . . .2.5 L . . .5.0 L . . .7.5 L . . .14 L

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BioFlo 310 Vessel Dimensions, Options and Accessories

VESSEL DIMENSIONS

VESSEL

Total Volume (L)

With Exhaust Condenser

Without Exhaust Condenser

NEW BIOFLO 310 FERMENTOR VESSEL HEADPLATE PORTS Vessel Size 2.5 L 5.0 L 7.5 L 14.0 L Catalog No. 6mm 1 3 3 3 13mm 9 10 12 12 Description 19mm 0 1 1 1 Total Ports 10 14 16 16

Length

Inches

Width

Inches

Height

Inches

Length

Inches

Width

Inches

Height

Inches

2.5 L 5.0 L 7.5 L 14.0 L

8 9.5 10.5 11.75

8 9.5 10.5 11.75

20.25 23 24 26.5 Description

8 9.5 10.5 11.75

8 9.5 10.5 11.75

16.25 18.63 20 23.5

Catalog No.

Cell Culture Conversion Kit/Gas Overlay M1287-3501 Microbial to Cell Culture Conversion Kit adapts the BioFlo 310 fermentor to enable high performance growth of mammalian, insect or plant cells using your existing CelliGen Plus or future CelliGen 310 vessel. Includes a TMFC with 4 gas mix which plugs into your BioFlo 310 cabinet, reducing the flow rate from 0 - 20 SLPM to 0 - 5 SLPM. Enables dual control for sparge and gas overlay. For Cell Culture Vessel Assemblies & Impellers, see page 52. 2nd DO-pH/Redox Options M1287-3530 2nd DO-pH/Redox Controller (Field Installed) Additional Options M1287-0020 RS232 8-Port (Serial) Controller (required when connecting scales) P0440-4489 Mettler Toledo Scale Display (required with each of the scales below) P0440-4325 Mettler Toledo Scale, 15 Kg (33.0 lbs) P0440-4321 Mettler Toledo Scale, 32 Kg (70.5 lbs) P0440-4326 Mettler Toledo Scale, 60 Kg (132.2 lbs) P0440-4327 Mettler Toledo Scale, 150 Kg (330.4 lbs) M1287-9966 Barnant Variable Speed Pump, 115V (connects via Analog I /O) 0 - 100 rpm M1287-9967 Barnant Variable Speed Pump, 230V (connects via Analog I/O) 0 - 100 rpm M1273-5001 Water Regulator/Filter Kit (4 Manifolds) M1273-5002 Air Regulator/Filter Kit (4 Manifolds) Replacement Glass Vessels M1287-9930 Replacement Vessel, 2.5 L M1287-9931 Replacement Vessel, 5.0 L M1287-9932 Replacement Vessel, 7.5 L M1287-9933 Replacement Vessel, 14 L Validation Kits M1287-0101 M1287-0102 Basic Validation Basic Plus Validation

Additional Options M1287-5042 Sampling Assembly (included in all vessel kits) M1287-9964 Optional Sampling Assembly adapter (used with Corning 15 ml or BD Falcon 50 ml disposable vials) M1287-5031 Septum Adapter Kit (Includes 10 Septa) M1287-5030 Probe Adapter Kit (12 mm compression) M1287-5034 6 mm Adapter Kit (Holds 1/4" Tube) M1287-9914 User's Kit for 2.5, 5.0 and 7.5 L - Includes tubings, clamps, filters, connectors, addition vessels and other commonly required items M1287-9915 User's Kit for 14 L, same as above M1287-6020 Spare Parts Kit, 2.5 L M1287-6021 Spare Parts Kit, 5.0 L M1287-6022 Spare Parts Kit, 7.5 L M1287-6023 Spare Parts Kit, 14 L M1273-9989 Addition Bottle Kit, 250 ml M1273-9990 Addition Bottle Kit, 500 ml M1287-9212 Tri-Port Addition Kit M1287-5043 Single Addition Kit Optional Autoclave Rack XMF-8624 Angled Autoclave Rack M1227-9231 Angled Autoclave Rack, low profile for 14 L vessels Autoclave Dimensions Catalog No. Vessel Assembly

1.6 L XMF-8624 3.3 L 6.6 L 14.0 L M1227-9231 14.0 L

Maximum Height Rack Mounted

11" (28 cm)

Maximum Depth Rack Mounted

20" (47.9 cm)

131/4" (33.7 cm) 15" 183/4" (38.1 cm) (47.7 cm) cm)

2115/16" (55.7 cm) 241/8" 26 " 26" (61.3 cm) (66.1 cm) (66.1 cm)

173/16" (43.7

Upgrade your existing BioFlo 3000 vessel or existing cabinet to a BioFlo 310. You can gain the power of the advanced BioFlo 310 controller while saving money by re-using your existing vessels/controller.

How to Order: To use your existing BF-3000 Vessel with a new BF-310 Cabinet: 1. Choose CABINET: (Includes Master Control Station with 1 TMFC, 0.4 - 20 SLPM ­ complete Fermentation cabinet) · 120V Master Control Station . . . . . . . . M1287-1003 · 230V Master Control Station . . . . . . . . M1287-1005 2. Choose Motor Conversion Kits: (Includes new motor, cables and tubing) · For BioFlo 3000 Vessels, 1 & 3 L . . . . . . M1287-1200 · For BioFlo 3000 Vessels, 6 L . . . . . . . . . M1287-1201 · For BioFlo 3000 Vessels, 14 L . . . . . . . . M1287-1202 To use your existing BF-3000 Cabinet with a new BF-310 Vessel: BioFlo 310 Retro Kit (Includes vessel assembly, vessel kit, tubing kit and motor) · For BioFlo 310 Vessels, 2.5 L . . . . . . . . M1287-1205 · For BioFlo 310 Vessels, 5.0 L . . . . . . . . M1287-1206 · For BioFlo 310 Vessels, 7.5 L . . . . . . . . M1287-1207 · For BioFlo 310 Vessels, 14 L . . . . . . . . . M1287-1208

1.732.287.1200 · 1.800.631.5417 · Fax: 1.732.287.4222 · [email protected]

51

Which Impeller is Best for Your Cell Line?

A Guide to Impeller Selection in Fermentors & Bioreactors

When growing Eukaryotes or Prokaryotes in a stirred-tank reactor, it's critical to choose the impeller type best suited to your process. This article provides a brief overview of our impellers and how they work, to guide you in selecting the best impeller for your application. For a table of commonly-used cell lines used in fermentation and cell culture processes, matched with the impeller(s) best suited for their growth, see our website at www.nbsc.com/papers or contact us for assistance. Cell Lift Impellers for Microcarrier Culture The Cell Lift Impeller, designed and patented by New Brunswick Scientific, provides uniform circulation for microcarrier cultures. This is an ultra-low shear impeller, in which the flow is caused by three discharge ports located on the impeller shaft. The rotation of the ports creates a low differential pressure at the base of the impeller tube, lifting the microcarriers up through the tube and expelling them out through the ports. This continuous recirculation loop keeps the cells uniformly dispersed throughout the vessel. Gases are introduced through a ring sparger, which generates bubbles that pass along the impeller between the exterior of the inner tube and an outer membrane, known as the aeration cage. A mesh lining, on the outer membrane of the cage, has 85 µm penetrations small enough to ensure that cells growing on the microcarriers cannot pass through. Gas exchange occurs at the membrane-media interface, ensuring that the cells remain in a bubble-free environment and are not subjected to shear due to bubble breakage. The bubbles are then expelled out the ports, located at the top of the impeller, into a second screened-in cage. A foam breaker directs air, supplied by a gas overlay, into the cage to break up any foam that has developed. The Cell Lift Impeller can be used in batch and fed batch processes of shear-sensitive animal cells. It can also used for continuous perfusion processes with the addition of a decanting column(s) and a media feed in/broth pump out setup. Packed-Bed Basket Impellers for Secreted Products Another unique New Brunswick Scientific design, the packed-bed basket impeller is used primarily for the manufacturing high yields of secreted products from anchorage-dependent or suspension cultures in perfusion. The basket is comprised of two horizontally-positioned, perforated metal screens that extend to the vessel walls. Enclosed between the screens, a bed of FibraCel® disks serves as a solid support matrix for cell growth. Cells growing in the disk bed become immobilized on or between the disks, where they remain throughout the culture run, protected from external shear forces. Media is circulated by way of a hollow impeller tube with discharge ports positioned above the basket. Like the Cell Lift impeller, rotation of these discharge ports creates a low differential pressure at the base of the impeller tube, which circulates the media throughout the system. The media receives gases through a sparger located at the bottom of the inner tube, protecting the cells from being exposed to the gas liquid interface. This results in low turbulence and low shear stress on the culture. Exceptionally high cell densities are achievable due to the high surface-to-volume ratio provided by the disk bed, coupled with the ability to use perfusion or medium replacement techniques. Culture periods in excess of three months have been reported. By ensuring the cells remain entrapped in the bed, this system also simplifies protein harvesting from the cell-free media. Rushton-Style Impellers Ideal for Fermentation The blades of Rushton and Rushton-type impellers are flat and set vertically along the agitation shaft, producing a unidirectional radial flow. These impellers are commonly used in fermentations of cell lines that require high oxygen rates, such as yeast, bacteria, and some fungi. Pitched Blade Impellers for Shear-Sensitive Microorganisms The blades on this impeller are flat, and set at approximately 45° angles, producing both an axial and radial flow. The combination provides better overall mixing, and creates a higher oxygen mass transfer rate (KLa) than that of unidirectional (axial or radial) flow impellers. Pitched blade impellers are low shear impellers, designed to gently mix the contents of the culture without causing cell damage. They are most commonly used with mammalian, insect, or other shear-sensitive cell lines, growing either in suspension or with the aid of microcarriers. They are also widely used in fermentation processes dealing with highly viscous cultures, such as filamentous bacteria and fungi, as well as some biofuels processes. Marine Blade Impellers for Shear-Sensitive Microorganisms The leading face of the blades on this impeller can be flat or concave, while the back sides are convex, producing an axial flow. Marine blade impellers are used for applications requiring gentle mixing without causing cell damage. Due to their unidirectional flow, however, the KLa of marine blade impellers tend to be slightly lower than that of impellers which employ both axial and radial mixing. Spin Filters for All Cell Lines Spin filters are retention devices commonly used to keep cells inside the vessel during continuous or perfusion culture. In NBS bioreactors, spin filters with a low-shear marine blade impeller are offered for suspension and microcarrier applications. The spin filter kit consists of a screened cage surrounding the impeller shaft, with very small filter pore openings that keep cells isolated outside the cage. Inside the rotating cage, a dip-tube is provided for continuous withdrawal of culture broth. A media feed tube is used outside the cage to provide a steady supply of fresh nutrients. NBS spin filters are offered with 10µm openings for suspension cultures and 75µm openings for microcarrier cultures. Due to its gentle mixing nature, the spin filter is typically used with cell lines that are microcarrier dependent or highly sensitive to shear. They are ideal for production of secreted proteins, as they keep the harvested media cell-free, and thereby simplify purification.

Cell Lift Impeller

Basket with FibraCel

Rushton-Type Impeller

Pitched Blade Impeller

Marine Blade Impeller

Spin Filter with Marine Blade

52

New Brunswick Scientific · a culture of innovation · www.nbsc.com

RPC Reactor Process Controller

RPC Reactor Process Controller, Used In BioFlo 310, 415 and 510 Fermentors & CelliGen 310 and 510 Bioreactors

Reactor Process Controller (RPC) is an advanced new controller used in all new NBS benchtop and pilot plant fermentors and bioreactors. By using the same controller in the BioFlo 310 & 415 & 510 fermentors, as well as CelliGen 310 & 510 bioreactors, you gain the advantages of easier scale-up, a reduced learning curve, and uniformity between NBS products. The system uses an advanced touchscreen to simplify setup, calibration and monitoring. RPC is capable of controlling up to 32 process loops per fermentor or bioreactor. When used in our BioFlo & CelliGen 310, it enables simultaneous control of up to 4 vessels -- and over 120 process loops -- from a single controller.

Summary Screen lets you conveniently view setpoints, current values, cascade loops and more -- for up to 10 parameters simultaneously. All 32 parameters can be viewed simply by scrolling up or down.

Synoptic View provides a pictorial alternative to the summary screen for viewing & editing process values.

Trend graphing makes it simple to track & export data on up to eight process variables per reactor over a six day span. Customize the view by selecting the parameters, colors & interval between sample measurements.

The setup screen lets you easily configure your settings -- Adjust gas flow options, change vessel size for more accurate PI values, save up to 10 recipes, add additional fermentors, and more.

Enter and view sterilization parameters and valve sequences from the sterilization screen shown, at left. (Available only on sterilizable-inplace 415 & 510 models). A variety of other screens are also provided for setting up and controlling pumps, alarms, cascade control strategies, and much more.

1.732.287.1200 · 1.800.631.5417 · Fax: 1.732.287.4222 · [email protected]

53

Dedicated Benchtop Cell Culture Bioreactors

CelliGen®310 Autoclavable Stirred-Tank/ Packed-Bed Bioreactor, 2.5 - 14 Liters

CelliGen 310 is a versatile benchtop bioreactor with an advanced controller and touchscreen interface capable of operating up to four reactors simultaneously. This compact system can operate in batch, fed batch or continuous mode and is suitable for growth of animal, insect and plant cells. Sophisticated Master Control Station: Runs on the powerful RPC (Reactor Process Controller) which can control up to 4 vessels, 32 control loops per reactor, 2 USB ports plus everything that is included in the Utility Station. Stores 10 recipes & can trend up to 8 parameters per reactor. (See page 53). Adjustable-position, 15" industrial color Touch Screen Display: is standard with the first bioreactor. Not required for 2nd, 3rd, and 4th reactor. Compact Utility Station: Each includes 3 built-in pumps, standard parameters (Agitation, Temperature, pH, DO, Foam/Level, plus Gas Loops depending on the gas selection) plus up to 7 analog inputs and 7 analog outputs, 1 TMFC* (0.1 - 5 SLPM**) with 4 solenoid valves and much more. Options: include multiple TMFCs with choice of 4 SLPM flow rates, 2nd DO ­ pH/Redox sensor and 8-port serial boxfor scales. Vessel Sizes: Interchangeable glass-jacketed vessels, 2.5, 5.0, 7.5 & 14 L total volumes. Impellers: choice of five types including patented low-shear NBS Cell Lift Impeller & unique packed-bed basket for high yields of secreted products - see below. Sensor Kit: Select pH/DO kits that include probe & cable. Basic or advanced validation packages are available.

Catalog No. Total Volume (L) Working Volume (L) Electrical Service

Trends 8 loops and up to 144 hours of process data.

System Includes

M1287-1260 M1287-1261 M1287-1262 M1287-1263 M1287-1264 M1287-1265 M1287-1266 M1287-1267

2.5 5.0 7.5 14.0 2.5 5.0 7.5 14.0

0.6 - 1.4 1.2 - 3.5 1.5 - 5.0 3.5 - 10.0 0.6 - 1.4 1.2 - 3.5 1.5 - 5.0 3.5 - 10.0

100 - 120V, 50/60 Hz 100 - 120V, 50/60 Hz 100 - 120V, 50/60 Hz 100 - 120V, 50/60 Hz 200 - 240V, 50/60 Hz 200 - 240V, 50/60 Hz 200 - 240V, 50/60 Hz 200 - 240V, 50/60 Hz

CelliGen 310 Master Control Station, 1 TMFC (0.1 - 5 SLPM) with 4-Gas Mixing, Touchscreen Display, Motor & Vessel Assembly

(*) TMFC = Thermal Mass Flow Controller (**) SLPM = Standard Liters Per Minute () When using a Cell Lift or Basket impeller, the minimum and maximum working volume is always the maximimum volume shown for that vessel size, in the above chart

How To Order:

1. 2. 3. 4. 5. 6. 7. Select Vessel Size, Electrical Services from above chart. Specify Impeller Kit - See article on page 52, then select part number from page 56 (mandatory). Specify Probe Kit - Select part number from page 55. (mandatory). Add FibraCel Disks - If basket impeller kit is selected. (For info on FibraCel, see page 40.) Optional Accessories - Choose from a large variety of standard accessories. (Pages 56, 63 - 66.) For a custom configuration or 2nd/3rd or 4th Utility Station, see page 55. If you want to upgrade your existing CelliGen Plus to a CelliGen 310, see page 55.

Impellers - See article on page 52 for more informaton

For high yields of secreted products from suspension or anchorage dependent cells. Perfusion only. Also for processes using viral infections, BEVS and gene therapy. Cells are immobilized on FibraCel disks and no special cell retention devices are required. For traditional vaccines as well as gene therapy processes using microcarriers, batch or perfusion. Perfusion requires a cell retention device such as a decanting column. Also for processes using viral infections. High KLa. For suspension cells in batch, fed-batch, or perfusion. In perfusion a cell retention device is required. Also for processes using viral infections. For suspension (10µ screen) and for microcarriers (75µ screen). Dip tube inside Spin Filter harvests cell-free medium. Perfusion only. For insect and other suspension cells as well as microcarriers in batch, fed-batch or perfusion. A cell retention device is required in perfusion. Also for processes using viral infections.

Basket Impeller with Fibracel® Disks Basket Impeller

Patented low-shear Cell Lift

Marine Blade

Spin Filter with Marine Blade

Pitched Blade

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New Brunswick Scientific · a culture of innovation · www.nbsc.com

How to order a CelliGen 310 with multiple Thermal Mass Flow Controllers (TMFCs) with various ranges, and/or 2nd, 3rd and 4th Utility Station, and/or 2nd DO - pH/Redox sensors

1. Choose voltage: · 100 - 120V . . . . . . . . . . . . . . . . . . . . . . . . . M1287-1010 · 200 - 240V . . . . . . . . . . . . . . . . . . . . . . . . . M1287-1020 2. Choose CABINET: (see description on page 52) · Master Control Station . . . . . . . . . . . . . . . . M1287-2110 · Utility Station with no display. . . . . . . . . . . M1287-2112 3. Choose GASES: · 0 TMFC (requires a Rotameter)*** . . . . . . . M1287-2010 · 1 Rotameter (0 - 20 SLPM) . . . . . . . . . . M1287-3510 · 1 Rotameter (0 - 5 SLPM) . . . . . . . . . . . M1287-3520 · 1 TMFC (0.4 - 20 SLPM)*** . . . . . . . . . . . . M1287-2012 · 1 TMFC (0 - 1 SLPM)*** . . . . . . . . . . . . . . . M1287-2019 · 1 TMFC (0.1- 5 SLPM)*** . . . . . . . . . . . . . . M1287-2011 · 2 TMFC (0.1- 5 SLPM) . . . . . . . . . . . . . . . . . M1287-2013 · 2 TMFC (0.4 - 20 SLPM) . . . . . . . . . . . . . . . M1287-2014 · 3 TMFC (0.1 - 5 SLPM) . . . . . . . . . . . . . . . . M1287-2015 · 3 TMFC (0.4 - 20 SLPM) . . . . . . . . . . . . . . . M1287-2016 · 4 TMFC (0 - 0.5 SLPM) . . . . . . . . . . . . . . . . M1287-2021 · 4 TMFC (0 - 1 SLPM) . . . . . . . . . . . . . . . . . M1287-2020 · 4 TMFC (0.1 - 5 SLPM) . . . . . . . . . . . . . . . . M1287-2017 · 4 TMFC (0.4 - 20 SLPM) . . . . . . . . . . . . . . . M1287-2018 3a. When should you select multiple TMFCs? When you require each gas to be individually contolled, monitored and displayed in SLPM. 3b. Which TMFC is right for your process? In Cell Culture Process ­ Refer to chart below. Processes might vary depending on your requirement. All gases combined should add up to the total gas range desired. 4. Vessel Kits: ADD-A-VESSEL KITS Vessel Size Total Volume (L) Includes Motor Catalog No. Without Motor Catalog No.

2.5 5.0 7.5 14.0

M1287-0310 M1287-0311 M1287-0312 M1287-0313

M1287-0330 M1287-0331 M1287-0332 M1287-0333

5. Select Impeller Kit (see page 54. 6. 2nd DO - pH/Redox board: Factory installed . . . . . . . . . . . . . . . . . . . . M1287-3540 7. Select pH/D.O. Kit (below) pH/DO Probe Kits - for all Impellers except Basket M1287-0400 M1287-0401 M1287-0402 M1287-0403 2.5 L 5.0 L 7.5 L 14 L

pH/DO Probe Kits - for Basket Impellers Only M1287-0674 M1287-0675 M1287-0676 2.5/5.0 L 7.5 L 14 L

Typical Quantity of FibraCel Used:

FibraCel Disk Specifications

Disk Diameter Autoclavable Endotoxin Tested Bioburden Tested Cytotoxicity Tested

Packed with power, to regulate one to four bioreactors from a single controller.

6 mm Yes Yes Yes Yes

FibraStage 2.5 L Vessel 5.0 L Vessel 7.5 L Vessel 14. L Vessel

10 Grams 50 Grams 150 Grams 250 Grams 500 Grams

Total gases when using different impellers and vessel sizes in CelliGen 310 [SLPM] Impellers Packed-Bed Basket Pitched / Marine Blade Spin Filter Cell Lift

(*) TMFC = Thermal Mass Flow Controller

Total Volume: 2.5 L Min Max 0 0.1 0 0.3 0 0.5 0 1.0

Total Volume: 5.0 L Min Max 0 0.15 0 0.5 0 0.5 0 1.5

Total Volume: 7.5 L Min Max 0 0.2 0 0.5 0 0.5 0 2.5

Total Volume: 14.0 L Min Max 0 0.3 0 0.7 0 1.0 0 5.0

(**) SLPM = Standard Liters per Minute

(***) 0 and 1 TMFCs are built with 4-gas manifold for 4 gas mixing

Upgrade your existing CelliGen Plus vessel or existing cabinet to a CelliGen 310. You can gain the power of the advanced CelliGen 310 controller while saving money by re-using your existing vessels/controller.

How to Order: To use a CelliGen 310 Cabinet with a your existing CelliGen Plus Vessel: 1. Choose CABINET: (Includes Master Control Station with 1 TMFC, 0.1 - 5 SLPM ­ complete Cell Culture cabinet) · 120V Master Control Station . . . . . . . . M1287-1002 · 230V Master Control Station . . . . . . . . M1287-1004 2. Choose Motor Conversion Kits: (Includes new motor, cables and tubing) · For CelliGen Plus Vessels, 2.2 & 5 L . . . . M1287-1203 · For CelliGen Plus Vessels, 7 & 14 L . . . . M1287-1204 To use your existing CelliGen Plus Cabinet with a new CelliGen 310 Vessel: CelliGen 310 Retro Kit (Includes vessel assembly, vessel kit, tubing & motor). · For CelliGen 310 Vessels, 2.5 L . . . . . . M1287-1210 · For CelliGen 310 Vessels, 5.0 L . . . . . . . M1287-1211 · For CelliGen 310 Vessels, 7.5 L . . . . . . . M1287-1212 · For CelliGen 310 Vessels, 14 L . . . . . . . M1287-1213

1.732.287.1200 · 1.800.631.5417 · Fax: 1.732.287.4222 · [email protected]

55

Dedicated Benchtop Cell Culture Bioreactors

CelliGen 310 Options & Accessories

Catalog No. Description Total Volume Catalog No. Description Total Volume 5.0 L 7.5 L 14 L 2.5 L 5.0 L 7.5 L 14 L 2.5 L 5.0 L 7.5 L 14 L Gas Overlay M1287-3500 Gas Overlay with TMFC (0.1 - 5 SLPM) M1287-3550 Gas Overlay without TMFC (requires rotameter) M1287-3505* Gas Overlay Vessel Kit (includes stainless steel tube & adapter, filter and silicone tubing). Scales M1287-0020 P0440-4489 P0440-4325 P0440-4321 P0440-4326 P0440-4327 Impeller Kits (includes Shaft, Impeller & Sparger) continued M1287-1141 Basket Impeller Kit for secreted proteins M1287-1142 Basket Impeller Kit for secreted proteins M1287-1143 Basket Impeller Kit for secreted proteins M1287-1125 Spin Filter Impeller Kit - for suspension cells, (10µ ) M1287-1126 Spin Filter Impeller Kit - for suspension cells, (10µ ) M1287-1127 Spin Filter Impeller Kit - for suspension cells, (10µ ) M1287-1128 Spin Filter Impeller Kit - for suspension cells, (10µ ) M1287-1135 Spin Filter Impeller Kit - for microcarriers M1287-1136 Spin Filter Impeller Kit - for microcarriers, (75µ ) M1287-1137 Spin Filter Impeller Kit - for microcarriers, (75µ ) M1287-1138 Spin Filter Impeller Kit - for microcarriers, (75µ ) Impeller Only M1287-9227 M1287-9228 M1287-9229 M1287-9230 M1287-9287 M1287-9288 M1287-9289 M1287-9290 M1287-9950 M1287-9952 M1287-9954 M1287-9951 M1287-9953 M1287-9955

RS232 8-Port (Serial) Controller (required when connecting 1- 8 scales to CelliGen 310) Mettler Toledo Scale Display (required with each of the above scales) Mettler Toledo Scale, 15 Kg (33.0 lbs) Mettler Toledo Scale, 32 Kg (70.5 lbs) Mettler Toledo Scale, 60 Kg (132.2 lbs) Mettler Toledo Scale, 150 Kg (330.4 lbs)

Replacement Glass Vessels M1287-9920 2.5 L Glass Vessel M1287-9921 5.0 L Glass Vessel M1287-9922 7.5 L Glass Vessel M1287-9923 14 L Glass Vessel Additional Options M1287-9966 Barnant Variable Speed Pump, 115V (connects via Analog I /O) 0 - 100 rpm M1287-9967 Barnant Variable Speed Pump, 230V (connects via Analog I/O) 0 - 100 rpm M1273-5001 Water Regulator/Filter Kit (4 Manifolds) M1273-5002 Air Regulator/Filter Kit (4 Manifolds) Validation Kits M1287-0111 Basic Validation M1287-0114 Basic Plus Validation 2nd DO-pH/Redox Options M1287-3530 2nd DO-pH/Redox Controller (Field Installed) Spare Parts Kits M1287-6030 For 2.5 L Vessels M1287-6031 For 5.0 L Vessels M1287-6032 For 7.5 L Vessels M1287-6033 For 14 L Vessels Impeller Kits (includes Shaft, Impeller & Sparger) M1287-1110 Cell Lift Impeller Kit for microcarriers (85µ ) 2.5 L M1287-1111 Cell Lift Impeller Kit for microcarriers (85µ ) 5.0 L M1287-1112 Cell Lift Impeller Kit for microcarriers (85µ ) 7.5 L M1287-1113 Cell Lift Impeller Kit for microcarriers (85µ ) 14 L M1287-1150 Air Wash Kit for increasing oxygen transfer rate. (Used with Cell Lift only) All Sizes M1287-5072 Marine Blade Impeller Kit for suspension cells 2.5 L M1287-5073 Marine Blade Impeller Kit for suspension cells 5.0 L M1287-5074 Marine Blade Impeller Kit for suspension cells 7.5 L M1287-5075 Marine Blade Impeller Kit for suspension cells 14 L M1287-5068 Pitched Blade Impeller Kit for suspension cells 2.5 L M1287-5069 Pitched Blade Impeller Kit for suspension cells 5.0 L M1287-5070 Pitched Blade Impeller Kit for suspension cells 7.5 L M1287-5071 Pitched Blade Impeller Kit for suspension cells 14 L M1287-1140 Basket Impeller Kit for secreted proteins Need to order FibraCel separately 2.5 L

() Includes dip tube. (*) Recommended when purchasing overlay.

Pitched Blade Impeller only, Right-hand 2.5 L Pitched Blade impeller only, Right-hand 5.0 L Pitched Blade impeller only, Right-hand 7.5 L Pitched Blade impeller only, Right-hand 14 L Pitched Blade impeller only, Left-hand 2.5 L Pitched Blade impeller only, Left-hand 5.0 L Pitched Blade impeller only, Left-hand 7.5 L Pitched Blade impeller only, Left-hand 14 L Marine Blade Impeller only, Right-hand pitch (directs flow up). 2.5 & 5.0 L Marine Blade Impeller only, Right-hand pitch (directs flow up). 7.5 L Marine Blade Impeller only, Right-hand pitch (directs flow up). 14 L Marine Blade Impeller only, Left-hand pitch (directs flow down). 2.5 & 5.0 L Marine Blade Impeller only, Left-hand pitch (directs flow down). 7.5 L Marine Blade Impeller only, Left-hand pitch (directs flow down). 14 L

Microspargers M1287-5010 M1287-5011 M1287-5012 M1287-5013

Microsparger (5 micron) Microsparger (5micron) Microsparger (5 micron) Microsparger (5 micron)

2.5 L 5.0 L 7.5 L 14 L

Additional Options M1287-5042 Sampling Assembly, used with cell lift impeller M1287-1190 Decanter Assembly, used with cell lift impeller M1287-1191 Decanter Assembly, used with cell lift impeller M1287-1192 Decanter Assembly, used with cell lift impeller M1287-1193 Decanter Assembly, used with cell lift impeller Replacement Screen & O-Ring Kit for Cell Lift Impeller M1287-9985 For 2.5 L vessel M1287-9986 For 5 L vessel M1287-9987 For 7.5 L vessel M1287-9988 For 14 L vessel CelliGen 310 Perfusion Kits M1287-1185 2.5 L CelliGen 310 Perfusion Kit M1287-1186 5.0 L CelliGen 310 Perfusion Kit M1287-1187 7.5 L CelliGen 310 Perfusion Kit M1287-1188 14 L CelliGen 310 Perfusion Kit

2.5 L 5.0 L 7.5 L 14 L

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New Brunswick Scientific · a culture of innovation · www.nbsc.com

CelliGen Plus® Accessories

Catalog No. Description Total Volume Catalog No. Microsparger M1228-5027 M1228-5028 M1228-5029 M1228-5026 Perfusion Kits M1176-0120 Description Total Volume 2.5 L 2.5 L 5L 7.5 L Impeller Kits (includes Shaft, Impeller & Sparger) M1176-1000 Cell Lift Impeller Kit for microcarriers 2.5 L M1176-1001 Cell Lift Impeller Kit for microcarriers 5.0 L M1176-1002 Cell Lift Impeller Kit for microcarriers 7.5 L M1228-1014 Cell Lift Impeller Kit for microcarriers 14 L M1176-1020 Cell Lift Impeller Kit with stainless steel screens 5.0 L M1176-1030 Cell Lift Impeller Kit with stainless steel screens. 7.5 L M1228-0022 Air Wash Kit for increasing oxygen transfer rate. (Used with Cell Lift only) 2.5 L M1228-0021 Air Wash Kit (used with Cell Lift only) 5.0 L M1228-0020 Air Wash Kit (used with Cell Lift only) 7.5 L M1228-0023 Air Wash Kit (used with Cell Lift only) 14 L M1176-1003 Marine Blade Impeller Kit for suspension cells 2.5 L M1176-1004 Marine Blade Impeller Kit for suspension cells 5.0 L M1176-1005 Marine Blade Impeller Kit for suspension cells 7.5 L M1228-1013 Marine Blade Impeller Kit for suspension cells 14 L M1176-1006 Pitched Blade Impeller Kit for suspension cells 2.5 L M1176-1007 Pitched Blade Impeller Kit for suspension cells 5.0 L M1176-1008 M1228-1012 M1176-1012 M1176-1013 M1176-1016 M1228-1015 M1228-3002 M1228-3005 M1228-3007 M1228-3014 M1228-3012 M1228-3015 M1228-3017 M1228-3024 Impeller Only P0200-2050 P0200-2410 P0200-2600 P0200-2367 P0200-2227 P0200-2427 M1176-9351 M1176-9352 M1228-9209 Pitched Blade Impeller Kit for suspension cells Pitched Blade Impeller Kit for suspension cells Basket Impeller Kit for secreted proteins Need to order FibraCel separately Basket Impeller Kit for secreted proteins Basket Impeller Kit for secreted proteins Basket Impeller Kit for secreted proteins Spin Filter Impeller Kit - for suspension cells Spin Filter Impeller Kit - for suspension cells Spin Filter Impeller Kit - for suspension cells Spin Filter Impeller Kit - for suspension cells Spin Filter Impeller Kit - for microcarriers Spin Filter Impeller Kit - for microcarriers Spin Filter Impeller Kit - for microcarriers Spin Filter Impeller Kit - for microcarriers 14L 14 L 2.5 L 5.0 L 7.5 L 14 L 2.5 L 5.0 L 7.5 L 14 L 2.5 L 5.0 L 7.5 L 14 L

Sintered/Porous Microsparger (5 micron) Sintered/Porous Microsparger (40 micron) Sintered/Porous Microsparger (5 micron) Sintered/Porous Microsparger (5 micron)

Perfusion Kit for use with microcarrier applications. Includes nutrient and harvest reservoirs, decanting column, filters, tubing, clamps, and fittings Perfusion Kit same as above. Perfusion Kit same as above Perfusion Kit same as above Decanter Assembly for use with microcarriers Decanter Assembly Decanter Assembly Decanter Assembly

2.5 L 5L 7.5 L 14 L 2.5 L 5L 7.5 L 14 L

M1228-0120 M1228-0121 M1228-0122 M1176-5200 M1228-5202 M1228-5203 M1228-5200

Fibracel Disks® For Use In Basket (For more info, see page 36) M1292-9984 FibraCel Disks, 50 grams M1292-9988 FibraCel Disks, 250 grams Optional Bottom dO2 Kits (For Oxygen uptake rate measurement in basket impeller only) M1176-0315 Mettler Ingold Bottom D.O. Kit for 2.2 L Basket. Allows real-time measurement of O2 uptake rate M1176-0335 Same for 5.0 L Basket M1176-0350 Same for 7.5 L Basket M1228-0310 Same for 14 L Basket pH Port Adapters & Gaskets (Adapters enable PG 13.5 mm threaded probes to be screwed into NPT ports) M1016-0890 Gasket for probe adapters M1016-0900 & M1226-9446 M1016-0900 Adapter for 3/8" diameter NPT port M1226-9446 Adapter, 3-5/8" Long, for 3/8" diameter NPT port M1228-9401 Adapter for 1/2" diameter NPT ports M1228-9403 Adapter for 1" diameter NPT ports M1228-9409 Adapter for 2" diameter NPT ports M1228-9404 Adapter for 2-1/2" diameter NPT ports dO2 Port Length Extenders M1176-9540 M1176-9575 M1176-9579 Extender for 1-11/16" diameter ports Extender for 3-1/32" diameter ports Extender for 3-1/4" diameter ports

Marine Blade Impeller 3" (7.6 cm) diameter. Right-hand pitch (directs flow up). Marine Blade Impeller 3" (7.6 cm) diameter Left-hand pitch (directs flow down). Marine Blade Impeller 2.5" (6.4 cm) diameter Left-handed (directs flow down). Marine Blade impeller 2.5" (6.4 cm) diameter. Right handed (directs flow up) Marine Blade Impeller 4.5" diameter Right-handed (directs flow up) Marine Blade Impeller 4.5" diameter Left-handed (directs flow down) Pitched Blade Impeller only Pitched Blade impeller only Pitched Blade impeller only

5.0 & 7.5 L 5.0 & 7.5 L 2.5 L 2.5L 14 L 14 L 2.5 L 5.0 & 7 L 14 L

() Includes dip tube

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57

Fermentation & Cell Culture Systems

BioFlo® 415 Compact Sterilizable-In-Place Benchtop Fermentor for R&D, 7 - 19.5 Liters

Full featured lab fermentor with built-in- electric heater that utilizes your water supply to automatically achieve sterilization on your lab bench without the need for an external steam source. Applications: as a fermentor suitable for growth of bacteria, yeast and fungi. Can be adapted as a cell culture bioreactor with added components. Control Station: Runs on the powerful RPC (Reactor Process Controller) which can control up to 32 process loops, simultaneously trend 8 process parameters, and store up to 10 unique batch recipes. RPC come standard with integrated security features and the ability to control your process based on sophisticated cascades. (See page 53). The control station comes standard with 1 Thermal Mass Flow Controller (0.5 ­ 25 SLPM)* with 4 solenoid valves for 4 gas mixing, three built-in, fixed-speed pumps and seven analog inputs/outputs. Air/Gas Control: Replace the single TMFC** with a low-cost rotameter, or upgrade by adding additional TMFCs. 15" Touch-Screen Display: The control station comes with a bright, 15", adjustable-position industrial color touchscreen interface. The large, easy-toread screen facilitates effortless navigation of the integrated software. Vessel sizes: Interchangeable stainless-steel 7, 14, or 19.5L. Vessel/Drive Assembly: includes single mechanical seal with magnetic drive, motor, exhaust condenser and sparger assembly with SIP filters, foam/level probe kits and more. Impellers: 7 and 14 L vessel include 2 Rushton-type impellers and 19.5 L vessel includes 3 Rushton-type impellers. Options: include multiple TMFCs with choice of 4 different SLPM, Sampling Assembly, 2nd DO ­ pH/Redox sensors and 8-port serial box for scales. Pitched blade and marine blade impellers are also available.

Pre-Configured Kits Catalog Number TotalWorking Volume Volume Aspect Ratio Electrical Service

No Autoclave or Steam Generator Needed

M1360-1120 M1360-1121 M1360-1123

7L 14 L 19.5 L

2L-5L 4 L - 10 L 5 L - 15.5 L

2:1 2:1 3:1 100V - 240V, 50/60 Hz

System Includes Includes BioFlo 415 Control Station, 1 TMFC (0.5 ­ 25 SLPM) with 4-Gas Mixing, Touchscreen Display and Vessel Assembly Kit. Call for Detailed Quotation

How To Order:

1. Specify Pre-Configured Kit from above chart dependent on your vessel size 2. Specify cord set type (no additional charge): North America: M1360-0100. Elsewhere: M1360-0200 3. Select any of the highly recommended options from the list below. (Not included in the preconfigured kits) · Probe kits (pH, DO, Redox kits include probes and cables) · Baffle Plug Kit and Pitched or Marine Blade (required for cell culture process) · Needles and QMI 7-port Seputm Kit · Sample Assembly 4. For a different configuration than listed above, including multiple TMFCs with various ranges and/or 2nd DO- pH/Redox sensor: · Specify from the above cord set type (see item 2) and select Gases from list below: GASES: · 0 TMFC*** . . . . . . . . . . . . . . . . . M1360-2030 · 3 TMFC (0.5 - 25 SLPM) . . . . . . . . . . M1360-2036 · 1 TMFC (0.1- 5 SLPM)***. . . . . . . M1360-2031 · 4 TMFC (0 - 0.5 SLPM) . . . . . . . . . . . M1360-2041 · 1 TMFC (0.5 - 25 SLPM)*** . . . . . M1360-2032 · 4 TMFC (0 - 1.0 SLPM) . . . . . . . . . . . M1360-2040 · 2 TMFC (0.1- 5 SLPM). . . . . . . . . . M1360-2033 · 4 TMFC (0.1 - 5 SLPM) . . . . . . . . . . M1360-2037 · 2 TMFC (0.5- 25 SLPM). . . . . . . . . M1360-2034 · 4 TMFC (0.5 - 25 SLPM) . . . . . . . . . M1360-2038 · 3 TMFC (0.1 - 5 SLPM) M1360-2035 5. To upgrade your existing BioFlo 410 to a BioFlo 415: Choose cabinet M1360-1005 and specify cord set type (see item 2).

BioFlo 415 Options and Accessories

Catalog No. Description SPARE vessel WITH drive Assembly kits (Includes components listed in "Vessel/Drive Assembly" description, above). M1360-2020 7L System with Magnetic Drive M1360-2021 14L System with Magnetic Drive M1360-2023 19.5L System with Magnetic Drive M1360-2050 Fluidine Tank Bottom Drain/Harvest Valve Probe Kits 7 Liter Systems: M1294-9820 pH probe, Mettler Ingold Gel Filled M1294-9822 dO2 probe, Mettler Ingold M1360-9832 Redox probe, Hamilton Gel Filled 14 Liter Systems: M1294-9824 pH probe, Mettler Ingold Gel Filled M1294-9826 dO2 probe, Mettler Ingold M1360-9834 Redox probe, Hamilton Gel Filled 19.5 Liter Systems: M1294-9828 pH probe, Hamilton Gel Filled M1294-9830 D.O. probe, Hamilton M1360-9836 Redox probe, Hamilton Gel Filled Impellers M1273-9206 Pitched Blade Impeller (up flow), 7 L M1294-9940 Pitched Blade Impeller (up flow), 14 L M1294-9942 Pitched Blade Impeller (up flow), 19.5 L

(*) SLPM = Standard Liters per Minute (**) TMFC = Thermal Mass Flow Controller () If product received in 2006 or before, please contact NBS for correct part numbers.

Catalog No. Description Impellers (continued) M1273-9290 Pitched Blade Impeller (down flow), 7 L M1294-9941 Pitched Blade Impeller (down flow), 14 L M1294-9943 Pitched Blade Impeller (down flow), 19.5 L M1294-9948 Marine Blade Impeller, 7 L M1294-9944 Marine Blade Impeller, 14 L M1294-9945 Marine Blade Impeller, 19.5 L Additional Options M1294-5023 QMI 7-Port Septum Kit (19 mm port) P0720-6570 dO Probe Membrane Replacement Kit M1294-9544 PG 13.5 Probe-Adapter for 19 mm port M1294-9542 19 mm Probe-Adapter for 19 mm port M1273-5001 Water Regulator/Filter Kit (4 Manifolds) M1273-5002 Air Regulator/Filter Kit (4 Manifolds) P0440-0064 Needle (Pack of 100) 18 Gauge 1-1/2" Length (used for multiple septum ports) M1294-9954 Baffle Plug Kit (New vessel headplate) M1360-0101 Basic Validation M1360-0102 Basic Plus Validation M1360-0103 Enhanced Validation M1294-5013 Sampling Assembly M1360-9969 Spare Parts Kit

(***) 0 and 1 TMFC are built with 4-gas manifold for 4 gas mixing

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SIP Fermentation & Cell Culture Systems

BioFlo® 510 & CelliGen® 510 Sterilizable-In-Place Culture Systems, 19.5 and 40.0 Liters

The 510 culture systems, consisting of the CelliGen 510 Bioreactor and the BioFlo 510 Fermentor, offer high-quality, intermediate-sized systems capable of operating in batch, fed-batch, or continuous modes. Size/Construction: The 19.5L and 40.0L vessels are constructed out of electropolished 316L stainless steel and feature a height-todiameter ratio of 2:1. Applications: perfect for mammalian, insect, plant, yeast or bacterial cell cultures. Control Station: Runs on the powerful RPC (Reactor Process Controller) which can control up to 32 process loops, simultaneously trend 8 process parameters, and store up to 10 unique batch recipes. RPC come standard with integrated security features and the ability to control your process based on sophisticated cascades. (See page 53). The control station comes standard with three built-in, fixed-speed pumps, seven analog inputs/outputs, two USB ports, an integrated load cell and more. Air/Gas Control: Standard with one Thermal Mass Flow Controller (TMFC) and four solenoids for gas mixing. Replace the single TMFC with a low-cost rotameter, or upgrade the gas control by adding up to five TMFCs. 15" Touch-Screen Display: The control station comes with a bright, 15", adjustable-position industrial color touchscreen interface. The large, easy-to-read screen facilitates effortless navigation of the integrated software. Options: The 510 Culture Systems have modular designs featuring a wide array of standard and optional components. This modularity allows you the flexibility to add or remove options to meet your process requirements.

· · · · · · · · STANDARD FEATURES · User-Definable, Sterilization Sequence Program Built-in Touchscreen · Control System Security RPC (Reactor Process Control ) Software · Temperature Control Top-Mounted Drive with Double Mechanical Seal · Resterilizable Harvest Port ASME/CE Electropolished Vessel Baffles (Standard on BioFlo 510 Fermentation System) · Clean-In-Place Connection Port for Clean Steam Piping · Ring Sparger Load Cell · Inlet/Exhaust Filter Bright, 15" Industrial Color Touchscreen Controller · 7 Analog Inputs and 7 Analog Outputs 3-Fixed Speed Pumps

Bench or mobile system

How To Order: 1. Specify base system: vessel size 2. Select options from the list below. 3. Consult an NBS application specialist for your specific needs.

BioFlo 510 & CelliGen 510 Options

GAS FLOW OPTIONS · Rotameter: 0 - 64 SLPM 0 - 3 SLPM 0 - 32 SLPM · Various combinations of 2, 3, or 4 TMFCs: 0.06 - 3 SLPM 0.6 - 32 SLPM 0.3 - 15 SLPM 1 - 64 SLPM · Overlay with or without TMFC (0 - 15 or 0 - 32 SLPM) · Air Wash System with TMFC (0 - 15 or 0 - 32 SLPM) EXHAUST LINE OPTIONS · Exhaust Condenser · Automatic Pressure Control IMPELLER OPTIONS · Rushton Type - Used commonly for robust cell lines such as bacteria, yeast and algae, where maximum OTR is desired. Provided as standard on BioFlo 510 fermentors · Pitched Blade - Commonly used with mammalian, insect or other "shear sensitive" cell lines for batch, fed-batch, or continuous cultures. Produces axial and radial mixing · Pitched Blade with Magnetic Drive - For critical cell culture applications · Marine Blade - Commonly used with mammalian, insect or other "shear sensitive" cell lines for batch, fed-batch, or continuous cultures. Produces axial mixing · Spin Filter - For Suspension or Microcarrier cultures where a dip tube inside the filter withdraws cell media as harvest or waste · Cell Lift - Specially designed to provide uniform circulation for both Suspension and Microcarrier cultures. Can be used with optional decanting columns for perfusion cultures (40 L only) · Packed-Bed Basket Impeller - For secreted products from anchorage dependent and suspension cells, this impeller immobilizes cells in a bed of FibraCel® disks to provide extremely high cell densities (40 L only) PROBES · Single or Redundant pH/DO Control Systems · Redox Control System · Foam/Level Detection System

ADDITION AND SAMPLE OPTIONS · Resterilizable Sample Valve · Resterilizable Addition Valves (4 Maximum) · Resterilizable Addition/Harvest Valve with Diptube (2 Maximum) · 1 ½" Sanitary Fitting 7-Port Septum · Addition Vessels · Decanters · Sterile Sampling Assembly (Kit of 3) ADDITIONAL OPTIONS · Allen Bradley Compact Logix PLC Control System · Sprayballs for Clean in Place of Vessel · External Variable Speed Pumps · External Scales · 8-Port Serial Controller (RS-232) · Spare Parts Kit · Mobile Skid · Mobile Table · System Passivation SCADA SOFTWARE OPTIONS (see pages 64) · BioCommand® Lite · BioCommand Plus · BioCommand Plus with OPC VALIDATION OPTIONS · Basic Package · Basic Plus Package · Enhanced Package UTILITY REGULATOR & PRE-FILTER KIT OPTIONS · Process Air Pre-Filter/Regulator Kit · Instrument Air Pre-Filter/Regulator Kit · Water Pre-Filter/Regulator Kit · Process Steam Pre-Filter/Regulator Kit · Utility Steam Pre-Filter/Regulator Kit · Utility Connection Kit

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59

SIP Fermentation & Cell Culture Systems

Modular, Customizable SIP BioFlo® Pro Fermentors & CelliGen® Pro Bioreactors, 75 - 3000 Liters

Now featuring Allen Bradley CompactLogix Programmable Automation Controller

The New Standard In Large-Scale Customizable Bioprocess Equipment

New Brunswick Scientific's fully modular fermentors and bioreactors offer a unique solution for pilot through production-scale bioprocessing, combining quick delivery, dependable operation and system flexibility, all at an affordable price. The BioFlo Pro systems utilize industry-standard components for dependable operation, and an Allen Bradley programmable-logic controller (PLC) for easy integration into any production facility. The modularity of the system offers many options that can be added, removed, or changed at any time to meet your various process requirements. From validation packages to redundant probes, dual inlet/outlet filters, and multiple resterilizable-in-place addition valves, these systems have the options you need, at the price and delivery you require. Fermentors available in 75, 150, 300, 500, 1000, 1500 & 3000 liter sizes; Bioreactors available in 75, 150, 300 & 650 liter sizes.

Each standard system consists of:

· Allen Bradley CompactLogix Controller · 316 L ASME code/CE stainless-steel vessel: Fermentors, all sizes - 3:1 ratio -Bottom Drive Double Mechanical seal Bioreactors, 75 - 150 L - 1.9:1 ratio, 300 L - 1.6:1 ratio, 650 L - 2:1 ratio -Top Drive Double Mechanical Seal 75 - 300 L -Bottom Drive Double Mechanical Seal 650 L · Automatic Sterilization Program · Large Color Operator Interface Terminal · Open-Frame Piping Skid · Manual Air-Flow Control · Safety Features (Headplate open interlock, Emergency Stop, Rupture Disk, Jacket Relief Valve) · Automatic Temperature Control · SIP / CIP Drain Valve · SIP Inlet / Exhaust Filters · Automatic Agitation Control · Resterilizable Harvest Port · NEMA-4 Rated Electrical Cabinet

Multiple options available - see next page.

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SIP Fermentation Systems

BioFlo® Pro Fermentors 75L, 150L, 300L, 500L, 1000L, 1500L & 3000L

Now featuring the Allen Bradley CompactLogix Programmable Automation Controller How to Order:

1. Specify base system: vessel size 2. Select options from the list below. (Standard features listed on page 60) 3. Consult an NBS application specialist for your specific needs

BIOFLO PRO OPTIONS

AIR INLET LINE · Mass-Flow Controller · Dual-Inlet Air Filters (in series) · Dual-Inlet Air Filters with test integrity ports · Gas Overlay · 2-Gas Mix (O2 supplementation) EXHAUST LINE · Exhaust Condenser · Automatic Backpressure Control · Dual Exhaust Filters (in parallel) · Single or Dual Exhaust Filters with test integrity ports PUMPS · 4 Built-In Fixed-Speed Addition Pumps · Variable-Speed Pump (2 maximum) HOUSINGS/PROBES (Mettler Ingold) · pH / dO2 Probes and Transmitters · Redox Probe and Transmitter · Retractable Probe Housings · Redundant Probe Kits FOAM KITS · Foam Kit · High-Foam Kit · High-High Foam Kit FILTER/REGULATOR KITS · Process Steam · Utility Steam · Water · Instrument Air · Utility Connection Kit IMPELLERS · Rushton-type impellers are standard · Pitched Blade (Optional) · Marine Blade (Optional) ADDITION OR INOCULATION · Addition Valve (quantity up to 5) · 7- or 12-Port Septums · Addition Vessels - 4, 7.5, 11 & 19 L · Addition Vessel Scales SAMPLING · Sampling Valve (Resterilizable) · NovaseptumTM Sampling System* · Sterile Sampling Assembly (Kit of 3) ADDITIONAL ACCESSORIES · Clean-In-Place Option (transfer panel/spray balls) · Cooling by dedicated heat exchanger for chiller · Low-Seal Pressure Switch for Double-Mechanical Seal · Thermometer (Digital) · Electropolish · Passivation · Transfer Lines (Resterilizable) VESSEL VOLUME/WEIGHT OPTIONS · Vessel Volume via differential pressure · Load Cells · Level Probe DATA RECORDING OPTIONS · BioCommand® SCADA Software (see pages 64) · (7) Inputs (4-20 mA) for Ancillary Devices VALIDATION PACKAGES · Basic Validation · Basic Plus Validation · Enhanced Validation PARTS KITS · Spare Parts Kits · Preventative Maintenance Kits

(*) NA-Connect® and NovaseptumTM are registered trademarks of Millipore.

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61

SIP Cell Culture Systems

CelliGen® Pro Bioreactors 75L, 150L, 300L & 650L

Now featuring the Allen Bradly CompactLogix Programmable Automation Controller How to Order:

1. Specify base system: vessel size 2. Select options from the list below. (Standard features listed on page 60) 3. Consult an NBS application specialist for your specific needs

CELLIGEN PRO OPTIONS

AIR INLET LINE · 4-Gas Mix (CO2, O2, N2 and Air) · Single Mass-Flow Controller · 4 Mass Flow Controllers (CO2, O2, N2 and Air) · Dual-Inlet Air Filters (in series) · Dual-Inlet Filters with test integrity ports · Gas Overlay with TMFC · Air Wash · Overlay Valve Mass Flow Control EXHAUST LINE · Exhaust Condenser · Automatic Backpressure Control · Dual Exhaust Filters (in parallel) · Single or Dual Exhaust Filters with test integrity ports (in parallel) PUMPS · 4 Built-In Fixed-Speed Addition Pumps · Variable-Speed Pump (4 maximum) HOUSINGS/PROBES (Mettler Ingold) · pH / dO2 Probes and Transmitters · Redox Probe and Transmitter · Retractable Probe Housings · Redundant Probe Kits FOAM KITS · Foam Kit · High-Foam Kit · High-High Foam Kit FILTER/REGULATOR KITS · Process Steam · Utility Steam · Water · Instrument Air · Utility Connection Kit IMPELLERS Pitched Blade Standard (Suspension) · Marine Blade (Suspension) · Spin Filter (Suspension or Microcarrier) · Cell Lift (Microcarrier) · Packed-Bed Basket (Fibracel® Disks) · Utility Connection Kit ADDITION OR INOCULATION · 1/2" Addition Valve (quantity up to 3) · 1" Addition Valve · 7- or 12-Port Septums · Addition Vessels - 4, 7.5, 11 & 19 L · Addition Vessel Scales SAMPLING · Sampling Valve (Resterilizable) · NovaseptumTM Sampling System* · Sterile Sampling Assembly (Kit of 3) ADDITIONAL ACCESSORIES · Vessel Head Lift Assembly · Clean-In-Place Option (transfer panel/sprayballs) · Cooling by dedicated heat exchanger for chiller · Low-Seal Pressure Switch for Double-Mechanical Seal · Thermometer (Digital) · BioSep System Interface · Passivation · Transfer Line (Resterilizable) VESSEL VOLUME/WEIGHT OPTIONS · Vessel Volume via Differential Pressure (75 L - 300 L) · Load Cells · Level Probe DATA RECORDING OPTIONS · BioCommand® SCADA Software (see pages 64) · 7 Inputs (4-20 mA) for Ancillary Devices VALIDATION PACKAGES · Basic Validation · Basic Plus Validation · Enhanced Validation PARTS KITS · Spare Parts Kits · Preventative Maintenance Kits

(*) NA-Connect® and NovaseptumTM are registered trademarks of Millipore.

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Fermentor & Bioreactor Accessories

In addition to the accessories listed here, NBS can supply a variety of ancillary equipment which can interface with our fermentors and bioreactors. Please allow our process engineers to suggest reliable equipment to utilize in your process development, on-line analysis, up- and downstream processing. The following section presents only a selected list of the most commonly requested items.

Additional Accessories

Catalog No. Description Catalog No. Description Pumps And Accessories P0620-0653 Watson-Marlow 101 U/R, low capacity 1-32 rpm variable speed pump. Accepts external signals up to 60 V or 32 mA for automatic start/stop operation and reversible pumping by remote control, 120V 60Hz, 15 W P0620-0654 Same as above, but 220V-240 V, 50/60Hz, 15W P0620-0662 Watson-Marlow 101 F/R, low capacity 24 rpm fixed speed pump, 120V 60Hz, 25 W P0620-0664 Same as above, but 20 rpm, 220V-240V, 50/60Hz, 25W P0620-0920 Watson-Marlow 520 U/R, large capacity 1-220 rpm variable speed pump with front panel auto/manual control. Accepts external signals up to 60 V or 32 mA for automatic start/stop operation and reversible pumping by remote control, 120V 60Hz, 100W (Must be used with M1228-8001. See below) P0620-0921 Same as above, but 220V-240V, 50/60Hz, 100W (Must be used with M1228-8001. See below) P0620-2410A Masterflex Pump, fixed speed 30 rpm with Easy-Load II Pump head, 120V, 60Hz P0620-2411A Same as above, but 230V, 50Hz M1228-8001 External Control Cable for controller P0620-0920 or P0620-0921 through BioFlo 3000 console M1287-9966 Barnant Easy Load Pumps, 1-100 rpm variable speed pumps. 6 Ft. cable with 2 open wires to directly connect to BioFlo/CelliGen 310 or BioFlo 415 Analog I/O. 120V, 50/60 Hz M1287-9987 Same as above, but 220-230V, 50/60 Hz Note: Any fixed-speed pump can be used with the BioFlo 110/310/3000/415/4500 or CelliGen 310/Plus. Variable speed pumps may be remotely controlled using the BioFlo 4500, BioFlo 510, CelliGen 510, BioFlo Pro, General Purpose Controller or 4-20mA Interface Box. Call NBS for additional pump information Autoclavable Connectors P0240-1090 Female Glass Joint for sterile connection (One set requires male connector, listed below) P0240-1080 Male Glass Joint for sterile connection M1153-9633 Resterilizable Quick Connect, 19 mm for SIP units M1153-9639 Resterilizable Quick Connect, 25 mm for SIP units P0240-5000 Luer-lock Syringe Connector P0440-0061A 4-1/4" Hypodermic Needle, 8 gauge, (Dozen)

The numbers in the chart below identify which tubing is used by fermentor/bioreactor model. See the second chart for corresponding tubing part number, I.D. & O.D.

Fermentor/ Bioreactor BioFlo 110 BioFlo 310 & CelliGen 310 Vessel Gas Acid, Base Harvest Cooling Size Connectors Antifoam, Tube Coil Addition Port All All 1 10 8 8 5 5 5 ­ Single Addition Port 5 5 Triple Addition Port 8 8 Side Addition Port ­ ­

Silicone Tubing (Replacement) Item 1 2 3 4 5 6 7 8 9 10 Part Number P0740-3110 P0740-3111C3 P0740-2542 P0740-2445 P0740-2505 P0740-2590 P0740-2430 P0740-2396 P0740-2721C3 P0740-3113C3 I.D. 1/12" (2.0 mm) 1/8" (3.2 mm) 1/4" (6.4 mm) 1/8" (3.2 mm) 3/16" (4.8 mm) 5/16" (8.0 mm) 3/32" (2.4 mm) 1/16" (1.6 mm) 3/8" (9.5 mm) 5/32" (1.5 mm) O.D. 1/4" (6.4 mm) 1/4" (6.4 mm) 3/8" (9.8 mm) 1/4" (6.4 mm) 5/16" (8.0 mm) 7/16" (11.1 mm) 1/4" (6.4 mm) 3/16" (4.8 mm) 5/8" (16.6 mm) 1/4" (6.4 mm) Length 50' (17.8 m) 50' (17.8 m) 25' (8.9 m) 25' (8.9 m) 25' (8.9 m) 50' (17.8 m) 50' (17.8 m) 50' (17.8 m) 25' (8.9 m) 25' (8.9 m)

Neslab ThermoFlexTM Recirculating CFC-Free, Mobile Chiller

Recirculating chiller for use with low-to-high density cultures to maintain sub-ambient temperatures. Applications: for use with most NBS benchtop fermentors & bioreactors. Call for details. Cooling Capacity: 1400 Watt heat removal for the 120V, 60Hz unit & 1170 Watt heat removal capacity for the 230V, 50Hz unit, at 20°C. Features: positive-displacement pump, 1.9 gallon reservoir, digital controller display, level indicator, integrated funnel and air filter. Dimensions: 27.4" H x 14.2" W x 24.7" D (69.6 x 36.1 x 62.7 cm). Net weight: 126 lbs (57.2kg)

Model Catalog No. Electrical Service

Neslab ThermoFlex Recirculating Chiller

P0620-2796 Thermoflex 1400 PD 2, IPR plus PRV P0620-2798 Thermoflex 1400 PD 2, IPR plus PRV P0620-0959 Plumbing Package P0620-2799 Pressure Regulator (0-20 psi) M1287-9918 Pressure Gauge (0-30 psi)

120V 60 Hz 230V 50 Hz

NBS can also supply chillers for SIP fermentors/bioreactors. Call for details.

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63

Fermentor & Bioreactor Accessories

Catalog No. Description Catalog No. Description

Addition Vessels, Bottles, & Carboys (Stainless Steel) P0640-8070C 2 Gallon (7.6 L) Stainless Steel ASME Pressure Vessel for adding chemicals and nutrients. Wide-mouth closure with O-ring, 9" (23 cm) I.D, with two 3/8" (9.5 mm) FPT and two 1/4" (6.4 mm) FPT ports. Electropolished, with handle and metal skirt 5 Gallon (19 L) Stainless Steel ASME Pressure Vessel for adding chemicals and nutrients. Wide-mouth closure with O-ring, 9" (23 cm) I.D., with two 3/8" (9.5 mm) FPT and two 1/4" (6.4 mm) FPT ports. Electropolished, with handle and metal skirt

Chart Recorder Accessories P0540-1082 P0660-0106 Sequencer M1154-2395 M1154-2396 8 Channel Sequencer 16 Channel Sequencer Replacement Chart Paper 6 Color Replacement Ribbon

P0640-8060C

Chillers NBS can supply Chillers for most fermentors/bioreactors. Call for detailed information. See also page 63. Steam Generators NBS can supply utility and clean steam generators for most fermentors/bioreactors in various electrical services. Please call for part numbers and prices. Air Compressors NBS can supply oil-free air compressors and dryer systems for most fermentors/bioreactors in various electrical services. Please call for part numbers and prices. Sampling Probes (Cell Free)

(Polycarbonate) P0640-0470 250 mL, Polycarbonate Bottle with polypropylene screw closure, gasket. Autoclavable for addition of chemicals and medium Same as above, but 1 L Same as above, but 2 L Vented Polypropylene Closure for 250mL, 1 L & 2 L polycarbonate bottles only. Has two barbed ports and port caps. Accepts 11/4" (6.4mm) I.D. tubing.

P0640-0471 P0640-0480 P0280-2582

(Glass) XAR-100 M1228-5016 M1228-5014 M1052-0760 250 ml Erlenmeyer Glass reservoir for acid, base, antifoam, complete with silicon stopper 500 ml Reservoir, glass with vented aseptic addition cap 2 L Bottle Kit, glass, with aseptic reservoir cap 13.3 L (3.5 Gallons) Medium Reservoir Assembly (glass carboy, stopper, penetrations, sampler, filter, transfer line and tubing) for bacterial systems only 13.3 L (3.5 Gallons) Product Reservoir Assembly (glass carboy, stopper, penetrations, sampler, filter and transfer line) for bacterial systems only

Withdraws sterile, cell-free samples from bioreactor/ fermentor for either off-line or on-line analysis. Please call for part numbers and prices. Light Sources NBS offers special cold light sources in wave lengths from 300 800 nm and with various illumination intensities. Call for detailed information. Biochemistry Analyzers NBS will assist customers in the selection of off-line or on-line process analysis equipment and interfacing to any NBS fermentor/bioreactor for analysis of glucose, lactate, glutamate, ethanol, sucrose, lactose, galactose, choline, hydrogen peroxide and methanol. Call for details on these analyzers and how they can be interfaced with your fermentor/ bioreactor and BioCommand® software.

M1052-0700

Carboys (Glass) M1052-3170 13.3 L (3.5 Gallons) Nutrient Reservoir (autoclavable glass carboy with stopper, 3 penetrations and dip tube) for animal cell culture only M1052-3180 13.3 L (3.5 Gallons) Harvest Reservoir (autoclavable glass carboy with stopper and 2 penetrations) for animal cell culture only

Sample Vials M1227-9935 P0640-0500 25 mL Sample Vial & cap (Autoclavable, case of 72) 40 mL Sample Vial & cap (Autoclavable, case of 72)

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Fermentor & Bioreactor Accessories

General Purpose Controller For Fermentation & Cell Culture Process Control

NBS' General Purpose controller lets you incorporate data from almost any analytical instrument into your process, as well as control auxiliary pumps and valves, for total process optimization. Acting as a stand-alone controller, the General Purpose controller allows quick and simple connections to any piece of laboratory equipment that outputs or accepts 0 - 5 VDC, 0 - 10 VDC or 4 - 20 mA.

No. Input Types No. Output Types

4 4

4-20 mA 0-5 V or 0-10 V

5

4-20 mA, 0-5 V, and 0-10 V in any combination

The General Purpose Controller requires a computer and monitor for initial configuration and for use as a local user interface, if desired. Unit includes BC Local software, wall mount power transformer, and 6 foot RS-232 null modem cable. Software requires 486 computer or higher with unused COM port, SVGA monitor, Windows OS. Connects to NBS BioCommand Plus supervisory software or any industrial SCADA package via standard bus protocol.

Model Catalog No. Electrical Service 120V, 50/60Hz 220V, 50/60 Hz

General Purpose Controller

M1266-0000 M1266-0001

Interface Kit for RS232 Device

The RS232 Device Interface Kit provides the ability to integrate up to eight (8) simple RS232 devices into your fermentation process to obtain weight measurements, flow rates or other data. This information can be integrated directly into BioCommand Plus with OPC for the development of powerful feed strategies based on weights or pump flow rates. The kit includes one USB cable to connect to your PC, one eight-port RS232 serial box, and OPC server software designed specifically to communicate with BioCommand. This kit is designed to communicate with Mettler scales which use SICS level 0 communication protocol. Contact your local sales representative for details on connecting to other simple RS232 devices.

Model Catalog No. Electrical Service 120V, 50/60Hz 220V, 50/60 Hz

RS232 OPC Server Kit

M1295-0000 M1295-0001

EX-2000 Off-Gas Oxygen/Carbon Dioxide Monitor

The EX-2000 gas analyzer provides continuous and unattended monitoring of exhaust carbon dioxide (CO2) and oxygen (O2) gasses in benchtop fermentors & bioreactors. Applications: suitable for use with any size fermentor/bioreactor. Features: infrared (IR) sensor determines carbon dioxide concentration. Electrochemical sensor monitors the oxygen concentration. Factory calibrated. Signal Output: provides 4 - 20 mA output. Requires interfacing into ML-6100 or General Purpose or 4 - 20mA Multi-Loop Controller with BioCommand® software to allow for visualization and archiving of carbon dioxide and oxygen levels. BioCommand also allows for supervisory control and calculations. Construction: Enclosure material is corrosion-resistant fiberglass (NEMA 12); all external interface-plumbing components are 316 stainless steel. All necessary tubing and connection fittings are included for out-of-the-box installation. Dimensions: 13" W x 16.2" H x 8.07" D (33 x 41.1 x 20.5 cm). Net weight: 9 lbs. (4.1 kg). Contact NBS for system recommendations.

Model Catalog No. Electrical Service 100-240V 50/60 Hz

May be wall-mounted, or used on the benchtop. Shown with optional stand.

EX-2000 Monitor

Stand for bench mounting

M1276-0000

M1276-5000

NBS can also recommend and supply a variety of other systems for measurement of exhaust CO2 , O2 and other gases as well as systems for sample pre-treatment. Call for details on these analyzers and how they can be interfaced with your fermentor/bioreactor and BioCommand bioprocess software. () For temperature maintenance or for rapid cooling applications in larger fermentors and bioreactors, ask your NBS sales representative for assistance.

1.732.287.1200 · 1.800.631.5417 · Fax: 1.732.287.4222 · [email protected]

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Fermentation & Bioreactor Accessories

BioCommand® Plus Bioprocessing Software

Applications: Automates control on the basis of time or process events, archives data, and allows remote monitoring of up to 8 fermentors & bioreactors*. Enables batch/recipe management, batch summary, audit trail, user-level access control, and multiple tools for automating processes. System Requirements: Windows** XP Professional, 2000, or NT4.0 and CPU: At least a 350 MHz Pentium II microprocessor and 256MB RAM and at least 1 serial port. Includes necessary cables for connecting to one system. Features: · Create & save process "recipes" using simple tools designed for non-programmers · Archive records of batch data. View graphs of current vs. historical data · Authority levels are recipe-specific, assigned by the person defining the protocol · Monitor & control runs from multiple workstations (requires a LAN) · Built-in tools make programming easy. Customize a synoptic display to show process elements and current values. Define a process including comments, time profiles and more · Use measured & calculated values to implement automatic setpoint changes, nutrient feed schedules and more

Model Catalog No. M1291-0000

BioCommand Plus

BioCommand Plus Bioprocessing Software with OPC

NEW!

Applications: All the control features of BioCommand Plus, but for up to 16 fermentors & bioreactors (8 on AFS protocol / 8 Modbus protocol). System Requirements: Windows XP Professional, CPU: Pentium IV processor, 256 MB RAM minimum and at least 1 serial port. USB to RS 232 serial hub can be used to provide additional ports. Includes necessary cables for connecting to one system. Features - same as BioCommand Plus, but with these added capabilities: · True OPC compliant application. It can interface and connect to 3rd party equipment with an OPC server. · Can run up to 16 fermentors/ bioreactors using AFS and Modbus protocols on one system. (AFS/Modbus each require their own serial port). · Can provide connectivity to 3rd party SCADA such as Matlab, Labview and other OPC compliant packages. · Develop complex feeding strategies to control feed pumps based upon mass changes.

Model Catalog No. M1291-0010

BioCommand Plus with OPC

BioCommand Lite Data Logging Software

Applications: For basic data acquisition and remote control from the PC. Not programmable. Supports up to 8 independent fermentor/bioreactor processes*. Features: Includes one RS-232 cable for connection to one fermentor or bioreactor. When using multiple units, must order one Multi-Controller Adpter. Also order one RS-422 cable for each additional vessel. System Requirements: Windows** XP Professional, 2000, or NT4.0 and CPU: At least a 350 MHz Pentium II microprocessor and 256MB RAM and at least 1 serial port. Includes necessary cables for connecting to one system.

Model Catalog No. M1286-0000

BioCommand Lite

OPC Server

NEW!

Applications: For use with NBS Bioreactors and Fermentors. Features: · True OPC Compliant application. Can interface and connect to OPC compliant 3rd party equipment. · Can be used to provide connectivity to 3rd party SCADA such as MatLab, Labview and other OPC compliant packages. · Can be used without BioCommand OPC to provide connectivity to 3rd party SCADA such as MatLab, Labview and other OPC compliant packages.

Model Catalog No. M1291-0011

OPC Server

BioCommand Accessories

Catalog No. M1171-8010 M1286-8010 P0460-7550 M1286-0100 Description 50' RS-422 Cable, Serial cable to connect one fermentor or bioreactor controller. Length is approximately 50 feet (17 meters). Two or more may be joined if greater lengths are needed BioCommand Cable RS-232/RS-422 RS-232/RS-422 Converter Multi-Controller Connection Kit (contains M1171-8010, M1286-8010 & P0460-7550) for up to eight fermentor or bioreactor controllers to one PC. Maximum of five recommended in simultaneous operation

() Includes one M1286-0100 and RS-422 cable. Order one additional M1171-8010 cable for each additional controller. (**) Windows is a Trademark of Microsoft Corp. (*) We recommend a maximum of 5 fermentors/bioreactors be in simultaneous operation. When used with one BioFlo 110 PCU operating four vessels, each vessel is considered a separate fermentor/bioreactor.

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Superior Process Measurements for the BioTechnology Industry

Top Quality pH Sensors

· · · · Reliable and accurate measurements Liquid and gel-filled reference systems Advanced design to ensure longer life Fully autoclaveable and sterilizable

Innovative In-Situ CO2

· Most reliable and practical in-situ CO2 measurement system · Increase efficiency and product yield · Autoclavable and sterilizable sensor · Accurate and responsive measurements

Industry Standard DO

M300/M400

· Accepted leader in DO measurement · Precision-engineered anode/cathode assembly assures superior performance · Easiest rebuildable sensor available · 16 Ra/N5 surface finish or better

· · · ·

Easy Handling Intuitive user interface Enhanced process control Single and dual-channel version

Intelligent Sensor Management ISMTM

Versatile Optical Density

· Wide Linear measurement range for full fermentation cycle · Live, in-situ measurement of cell density · Virtual maintenance operation

· · · · · ·

"Plug and Measure" functionality Advanced sesnor diagnostics Predictive maintenance functions Dynamic Lifetime Indicator (DLI) Wear monitor* CIP/SIP Counter

INGOLD

Leading Process Analytical

visit WWW.mt.com/pro for more information

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Probes for Fermentation & Cell Culture

NBS can supply Spare Probes & Accessories from among the broad line of Mettler Ingold & Hamilton products. For spare probes, see below & pages 69 & 70. Directions for using these charts are shown below: · For pH probes see page 69. For dO2 probes see page 70. · Locate your vessel in the left column of the appropriate chart. · Slide your finger across to the right until you come to a box with a number in it, then look at the top of the column above the number to find the catalog number for that probe. NOTE: Vessels can accommodate more than one type of probe. Each time you see a number for a particular vessel, it indicates that probe can be used with that vessel. · The number inside the box refers to the associated reference charts for cables, adapter, and housings, which follow below. · Questions? Call Customer Service at 1.800.631.5417 or 1.732.287.1200. Or contact your local NBS sales representative.

Redox Probes Redox probes for BioFlo 110 require a Redox Transmitter

Mettler Ingold

Catalog Number Immersion Depth Vessel Connection Cable end Type BioFlo 110 BioFlo 110 BioFlo 110 BioFlo 310 BioFlo 310 BioFlo 310 BioFlo 410 / 415 BioFlo 410 / 415 BioFlo 410 / 415 BioFlo 510 BioFlo Pro 1.3 L & 3L 7.5 L 14.0 L 2.5 L 5.0 L & 7.5 L 14.0 L 7L 14 L 19 L all vessels all vessels 3, 7 & 9 4&6 4, 5 & 8 2 2 2 2 2 2 2 2 2 2 P0720-5780 120 mm PG 13.5 mm K8 Gel P0720-5781 200 mm PG 13.5 mm K8 Gel 1 1 1 1 1 P0720-5782 325 mm PG 13.5 mm K8 Gel P0720-5784 425 mm PG 13.5 mm K8 Gel P0720-6532 325 mm PG 13.5 mm K8 Gel

Hamilton

P0720-6531 425 mm PG 13.5 mm K8 Gel P0720-6530 625 mm PG 13.5 mm K8 Gel

Redox Cables and Housings referenced in table above

Ref. Catalog No. Description Cables referenced in table above

1 2 3 4 5 P0720-2763 P0720-2275 P0720-2277 M1290-0610 M1290-8012 Redox Cable, 110 Redox Cable, BioFlo 310, 410 and 415 Redox Cable, BioFlo 510 pH/Redox Cable for BioFlo and CelliGen Pro pH/Redox Ground Wire Extention for Retractable Probe Housings (325mm)

Ref. Catalog No. Description Housings referenced in table above

6 7 8 9 P0720-6450C1 P0720-6240C3 P0720-5570C M1361-9208 25mm Ingold Port 12mm Housing with Material Certificate (BioFlo/CelliGen Pro Only) 25mm Ingold Port 12mm Housing with Material Certificate INTRAC 797, Stainless Steel Retractable Probe Housing, 25mm Ingold Port, 325mm Probe Length w/ Material Certificate 510 Port Adapter - Converts 1.5" Sanitary to 25mm Ingold Port

Redox Transmitters

Catalog No. P0620-5974 P0620-5975 Description Transmitter, Panel Mount, (required with BioFlo 110 Redox probes) Transmitter, Wall Mount, (required with BioFlo 110 Redox probes)

Optical Density (Turbidity) & CO2 Probes

Catalog No. Description Turbidity Probes

P0720-5950* P0720-5951* P0720-5961 P0720-5962 P0720-5963 P0720-5960 Turbidity Probe, Autoclavable 12mm/297mm Length InPro 8100 (Cable not included) Turbidity Probe, Autoclavable 12mm/120mm Length InPro 8100 (Cable not included) Turbidity Probe, SIP 12mm/120mm Length InPro 8200 with Cable Turbidity Probe, SIP 12mm/205mm Length InPro 8200 with Cable Turbidity Probe, SIP 12mm/297mm Length InPro 8200 with Cable Turbidity Probe, SIP 12mm/407mm Length InPro 8200 with Cable

Catalog No.

Description Turbidity Accessories

P0720-2430 P0620-5551 P0720-6480 P0720-6481 P0720-6482 P0620-6580 P0720-9660

5M Turbidity Cable Turbidity Transmitter, TRB 8300 CO2 Probes (VP Connector) CO2 Probe, 12mm / 120mm length CO2 Probe, 12mm / 220mm length CO2 Probe, 12mm / 320mm length CO2 Accessories CO2 Transmitter, 5100E CO2 Cable, VP CABLE VP6-ST/3M (9.9FT)

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Probes for Fermentation & Cell Culture

pH Probes

Mettler Ingold

Catalog Number Immersion Depth Vessel Connector Cable End Type BioFlo 110 / 3000 BioFlo 110 / 3000 BioFlo 110 / 3000 BioFlo 110 / 3000 BioFlo 310 BioFlo 310 BioFlo 310 CelliGen 310 CelliGen 310 CelliGen 310 CelliGen 310 Basket CelliGen 310 Basket CelliGen Plus CelliGen Plus CelliGen Plus CelliGen Plus Basket CelliGen Plus Basket CelliGen Plus Basket BioFlo 410 / 415 BioFlo 410 / 415 BioFlo 410 / 415 BioFlo 4500 BioFlo 5000 BioFlo/CelliGen 510 BioFlo/CelliGen Pro 1.3 L / 1.6 L 3L 7.5 L / 6.6 L 14.0 L 2.5 L 5.0 L & 7.0 L 14.0 L 2.5L 5.0 L & 7.5 L 14.0 L 2.5 L to 7.5 14.0 L 2.2 L 5.0 L & 7.5 L 14.0 L 2.2 L 5.0 L & 7.5 L 14.0 L 7.0 L 14.0 L 19.5 L all vessels all vessels all vessels all vessels 2&8 1&8 4, 8 & 10 5&7 5, 6 & 9 2&9 1&9 2 2 2 3 3 3 2 2 2 3 3 3 3 3 3 3 3 P0720-5581 120 mm 25 mm K9 Gel P0720-5582 200 mm PG 13.5 mm K9 Gel 2 2 2 2 P0720-5584 225 mm PG 13.5 mm K9 Gel P0720-5580 325 mm PG 13.5 mm K9 Gel P0720-5583 425 mm PG 13.5 mm K9 Gel

Hamilton

P0720-6540 625 mm PG 13.5 mm K9 Gel

() For CelliGen Probe Adapters, see page 57.

pH Probe Cables & Housings

Reference No. Catalog No. Description AK9 Cable for BioFlo 5000 AK9 cable, 3ft, BioFlo IIC, 2000, III, 110, 3000, IV, 4000, 4500, CelliGen and CelliGen Plus pH Cable for BioFlo 310, 410, 415 and CelliGen 310 pH Cable for BioFlo 510 and CelliGen 510 pH/Redox Cable for BioFlo and CelliGen Pro pH/Redox Ground Wire Extention for Retractable Probe Housings (325mmm) Cables referenced in table above 1 P0720-2093 2 P0720-2095 3 P0720-2273 4 P0720-2276 5 M1290-0610 6 M1290-8012

Cables for older NBS equipment, not referenced in table above M1131-8010 AK9 Cable, 8ft (2.4m). For Micros, MPP, MicroGen (ML-4100), MicroLift, and ML4100 P0720-2090 AK9 Cable, Analog For MultiGen, BioFlo I , MicroFerm and MicroGen M1238-8001 pH Cable for BioFlo 6000 (Ingold Probes Only) Housings referenced in table above 7 P0720-6450C1 25mm Ingold Port 12mm Probe Housing with Material Certificate (BioFlo/CelliGen Pro Only) 8 P0720-6240C3 25mm Ingold Port 12mm Probe Housing with Material Certificate 9 P0720-5570C INTRAC 797, Stainless Steel Retractable Probe Housing, 25mm Ingold Port, 325mm Probe Length w/ Material Certificate 10 M1361-9208 510 Port Adapter - Converts 1.5" Sanitary to 25mm Ingold Port

1.732.287.1200 · 1.800.631.5417 · Fax: 1.732.287.4222 · [email protected]

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Probes for Fermentation & Cell Culture

dO2 Probes

Mettler Ingold (InPro 6800)

Catalog Number Immersion Depth Vessel Connector Cable End BioFlo 110 a / 3000 b BioFlo 110 a/ 3000 b BioFlo 110 a / 3000 b BioFlo 110 a / 3000 b BioFlo 310 BioFlo 310 BioFlo 310 BioFlo 310 CelliGen 310 CelliGen 310 CelliGen 310 CelliGen 310 CelliGen Plus CelliGen Plus CelliGen Plus CelliGen Plus CelliGen Plus a / 310 b (Basket Only) CelliGen Plus a / 310 b (Basket Only) CelliGen Plus a / 310 b Basket Only) CelliGen Plus a / 310 b Basket Only) BioFlo 410 / 415 BioFlo 410 / 415 BioFlo 410 / 415 BioFlo 4500 BioFlo 5000 BioFlo/CelliGen 510 BioFlo/CelliGen Pro 1.3 L / 1.6 L 3.0 L / 3.3 L 7.5 L / 6.6 L 14.0 L 2.5 L 5.0 L 7.5 L 14.0 L 2.5 L 5.0 L 7.5 L

Hamilton

P0720-6282 220 mm PG 13.5 mm T82 1, 11 & 14 1, 12 & 14 1, & 13 5 5 5 5 5 5 5 5 1 & 13 1 1 P0720-6283 320 mm PG 13.5 mm T82 P0720-6284 420 mm PG 13.5 mm T82 P0720-6520 625 mm PG 13.5 mm T82

P0720-6270 80 mm 25 mm T82

P0720-6280 120 mm PG 13.5 mm T82

P0720-6281* 160 mm PG 13.5 mm VP 3 or 1

14.0 L 2.2 L 5.0 L 7.5 L

14.0 L

1 & 13 1 & 13 a / 5 b 1a / 5 b 1a / 5 b 1a / 5 b 5 5 5 1&8 2&8 1&8 2&8 6, 8 & 10 4&7 1&9 2&9 4&9 1a / 5 b 1a / 5 b 1a / 5 b

2.2 L / 2.5 L 5.0 L 7.5 L

14.0 L 7.0 L 14.0 L 19.5 L all vessels all vessels all vessels all vessels

dO2 Cables, Housings and Accessories (plus pH General Accessories)

Ref. Catalog No. Description Ref. Catalog No. Description Adapters not referenced in table above Cables referenced in table above -- M1176-9575 dO2 port length extender (same threads), 3-1/32" 1 P0720-2331 DO Cable, BioFlo IIC, III, 3000, 4000, IV, 4500, -- M1176-9579 dO2 port length extender (same threads), 3-1/4" 110 (except 1.6L) and CelliGen Plus Gasket referenced in table above 2 M1131-8009 DO Cable, BioFlo 5000, Micros, MicroLift, MPP 14 M1016-0890 Gasket for probe adapters M1016-0900 & M1226-9446 and ML-4100 3 P0720-2332* DO Cable, BioFlo 110 (1.3L) and BioFlo III/3000 (1.6L) dO2 Accessories, Mettler Ingold not referenced in table above -- P0620-5192 dO2 Amplifier & cables. Converts polarographic signal 4 P0720-2342 DO Cable, BioFlo and CelliGen Pro to galvanic for CelliGen, 120 V, 50/60 Hz. Not CE rated. 5 P0720-2333 DO Cable, BioFlo 310, 410, 415 and CelliGen 310 -- P0620-5193 Amplifier, same as above but 220 V 50/60 Hz 6 P0720-2336 DO Cable, BioFlo 510 -- P0620-5580 dO2 Transmitter Mettler Model 4500, 115 V Housings referenced in table above -- P0720-2560 dO2 Cable, 3 meters for Mettler Model 4500 7 P0720-6450C1 25mm Ingold Port 12mm Probe Housing with -- P0720-2561 dO2 Cable, 5 meters for Mettler Model 4500 Material Certificate (BioFlo/CelliGen Pro Only) -- P0720-5567 dO2 probe cap 8 P0720-6240C3 25mm Ingold Port 12mm Probe Housing with -- P0720-6268 dO2 Membrane Kit for Inpro 6000. Includes Material Certificate four membranes, O-rings, & 25 mL electrolyte 9 P0720-5570C INTRAC 797, Stainless Steel Retractable Probe Housing, -- P0720-6339 dO2 Membrane Kit for Inpro 6000. Includes 25mm Ingold Port, 325mm Probe Length one membrane, O-rings, & 25 mL electrolyte w/ Material Certificate -- P0720-6470* Probe Adapter, converts T-82 to VP connector 10 M1361-9208 510 Port Adapter - Converts 1.5" Sanitary to 25mm pH & dO2 General Accessories, not referenced in table above Ingold Port -- P0720-5750 Polarization module for constant preconditioning of Adapters referenced in table above sensor without amplifier electronics. Battery included 11 M1016-0900 PG13.5 thread adapter for 3/8" NPT port -- P0720-5631 pH & dO2 simulator/calibrator. For checking integrity 12 M1226-9446 PG13.5 thread adapter for 3/8"NPT port, 3-5/8" of pH & DO electronics & cables 13 M1176-9540 DO Port Lenth Extender (same threads), 1-11/16"

(*) 1L BioFlo 110 probes require a VP connector cable, P0720-2332, or probe adapter P0720-6470.

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Centrifugation Equipment

CEPA High-Speed Centrifuges

The CEPA family of centrifuges comprises a variety of systems used in the life sciences as well as in a wide range of other fields of industry. The high-speed models described in this catalog are for the life sciences market. They are designed for rapid cell removal and clarification of fermentation broth and similar liquid-solid mixtures in "clarifying" configurations; or for the separation of two liquids in "separating" configurations. This family of instruments range from a small lab unit, through large production-scale models capable of processing up to 3,000 L/hr. All models use bottom feed, retain solids in the bowl, expel supernatant from upper tray(s). All wetted surfaces are stainless steel (except optional silicone bottom valve). Basic configurations include one bowl, ancillary equipment1, and tools. Virtually all combinations of options are available on all models in all voltages.

LE Benchtop Model Laboratory Centrifuge Z41 Floor Model Centrifuge Z61 Floor Model Centrifuge Z81 Production Floor Model Centrifuge

Model

All model LEs feature variable speed and digital tachometer. Recommended for separating 2 - 15 liters of liquids with moderate to high2 solids content, and up to 30 liters for liquids with low solids content. Typical clarification rate is 10-20 L/hr. Recommended for laboratory or pilot-scale volumes of 20 - 75 L for liquids with high to moderate solids content1, and up to 500 L for low solids content liquids. Typical clarification rate is 50 - 250 L/hr. All model Z41s feature 3-phase electric motors for rapid acceleration. Recommended for pilot-scale volumes of 30 - 200 L for liquids with high to moderate solidscontent1 with maximum throughput of 1,500 L/hr for liquids with low-solid content. Typical clarification rate is 100 - 500 L/hr. All model Z61s feature 3-phase electric motors for rapid acceleration. All model Z81s feature 3-phase electric motors for rapid acceleration. Recommended for pilot and production process volumes of 50 - 500 L for liquids with high to moderate solids content1, and up to 2,000 L for liquids with low-solids content. Typical clarification rate is 150 - 1,500 L/hr. For larger capacity needs, ask your rep about Model Z101. Specifications Power: Bowl Volume: Rotational Speed: Max Acceleration: Height: Power: Bowl Volume: Rotational Speed: Max Acceleration: Height: Power: Bowl Volume: Rotational Speed: Max Acceleration: Height: Power: Bowl Volume: Rotational Speed: Max Acceleration: Height: 330 Watts, various voltages available 0.25 L 15,000 - 45,000 rpm 50,000 G 27" 0.9 kW, various voltages-specify 2L 20,000 rpm 17,000 G 46" 1.5 kW, various voltages-specify 6L 17,000 rpm 18,000 G 61" 2.2 kW, various voltages-specify 8L 16,000 rpm 18,000 G 61" Optional Features Recommended Accessories Spare Parts Kit, XLE-SP1 Extra clarifying cylinders Extra separating cylinders Cooling Coils Spare Parts Kit, X41-SP1 Extra clarifying cylinders Extra separating cylinders Cooling Coils Spare Parts Kit, X61-SP1 Extra clarifying cylinders Extra separating cylinders Cooling Coils Spare Parts Kit, X81-SP1 Extra clarifying cylinders Extra separating cylinders Cooling Coils

LE Z41

Z61

Z81

Clarifying Bowl: Retains solids such as biomass and discharges liquid through single-level upper discharge ports. Separating Bowl: Separates liquids of different densities, retains solids and discharges liquids through bi-level upper discharge ports. Closed Bowl (non-continuous): For precise sediment determination of measured charges. Contains no feed or discharge lines. Emulsifying Bowl: For emulsification of two liquids. Adjustable Separating Bowl: A separating cylinder with interchangeable separating rings for optimizing performance with different liquid combinations. Bottom Valve: Centrifugally actuated silicone rubber valve prevents centrifuge contents from emptying when cylinder rotation is stopped. The valve pre-

vents feed input when rotational speeds are less than 80% of maximum. Cooling Coil: Stainless steel. Allows circulation of cold water or other coolants to lower the temperature of the cylinder's contents during centrifugation. Enclosed Models: CEPA centrifuges are available mounted in cast-metal chambers with stainless steel-clad interiors. Sealed by a sturdy door the enclosure facilitates quieter operation, control of gaseous environment at atmospheric pressure. HEMA Configuration: CEPA centrifuges can be supplied with serumclarifying cylinders and full length cooling coils for blood fractionation. Explosion Proof: For use where risk of explosion exists.

(1) Ancillary equipment, which varies with the model, includes various cleaning tools such as special brushes and other implements. (2) Moderate to high solids content is defined as 50 g (or more) of cell paste per liter. Low solids content is less than 5 g per liter. Note: Centrifuges also available with different combinations of features than those listed here.

1.732.287.1200 · 1.800.631.5417 · Fax: 1.732.287.4222 · [email protected]

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NBS Equipment Service Plans

Protect Your Investment

NBS offers three service options to keep your equipment in peak operating condition, and your research & production schedules on track. We can accommodate virtually any budget, through a choice of Extended Warranty and preventive maintenance contracts, or On-Call Service. What we provide: · Electrical, electronic and mechanical safety checks · Problem analysis, complete functional check-up and testing of all systems · Functional checks of sensors and instrumentation Why choose NBS: · Our staff of experienced service professionals is the largest and best-trained in the industry · Our extensive parts inventory is strategically located around the country for rapid response to your needs · Our new, improved computerized system for service scheduling permits quality service from coast to coast · We provide a 20% discount on parts with preventive maintenance contracts & discounts for multiple items under contract

Extended Warranty

This plan is a one-year extension of the original equipment warranty, and includes parts and labor as needed. Which Equipment is Covered: All NBS Products. Length of Contract: Twelve months after the expiration of your original warranty. When to Purchase: At the time of your equipment purchase. Special Discounts: Ask your NBS sales representative for a quote.

Service Contracts

Service contracts are designed to keep NBS equipment in top-running condition. Which Equipment is Covered: Preventive maintenance is available for fermentation and cell culture equipment as well as freezers, CO2 incubators, media prep, centrifuges and shakers. For How Long: Twelve months. When to Purchase: Any time; products may be subject to NBS' equipment inspection and mutual acceptance. Contact your local NBS representative for details.

Repair Service On-Call

NBS also offers on-call repair service -- no contracts needed! In North America, simply contact our Customer Service Department at 1.800.631.5417, 8:15 - 6:00 pm Eastern Time, to order replacement parts or request on-site or in-house repair service. Elsewhere, contact your local NBS service office.

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New Brunswick Scientific · a culture of innovation · www.nbsc.com

Index by Catalog Number

NBS Part No. Page NBS Part No. Page NBS Part No. Page NBS Part No. Page ACE-1000S . . . . . . . . . . . 5 ACE-10S . . . . . . . . . . . . . 5 ACE-2000S . . . . . . . . . . . 5 ACE-3000 . . . . . . . . . . . . .5 ACE-4000S . . . . . . . . . . . 5 ACE-5000S . . . . . . . . . . . 5 ACE-6000S . . . . . . . . . . . 5 ACFE-2800S . . . . . . . . . . 5 ACSB-1000S . . . . . . . . . . 5 ACSB-500S . . . . . . . . . . . 5 AG-00 . . . . . . . . . . . . 13, 17 AG-1 . . . . . . . . . . . 7, 13, 17 AG-2 . . . . . . . . . . 7, 13, 17 AG2-00 . . . . . . . . . . . 12, 18 AG2-10 . . . . . . . . . . . 12, 18 AG-21-00 . . . . . . . . . . . . 13 AG-28 . . . . . . . . . . 7, 13, 17 AG2-UT . . . . . . . . . . 12, 18 AG-4 . . . . . . . . . . . 7, 13, 17 AG-6 . . . . . . . . . . . 7, 13, 17 AG7-125 . . . . . . . . . . 10, 19 AG7-250 . . . . . . . . . . 10, 19 AG7-50 . . . . . . . . . . . 10, 19 AG7-TT13 . . . . . . . . . 10, 19 AG7-TT16 . . . . . . . . . 10, 19 AG7-TT20 . . . . . . . . . 10, 19 AG7-TT25 . . . . . . . . . 10, 19 ATC-TT16 . . . . . . . . . . . . 32 ATC-TT25 . . . . . . . . . . . . 32 B50-SQ . . . . . . . . . . . . . .25 B75-SQ . . . . . . . . . . . . . .25 B95-SQ . . . . . . . . . . . . . .25 CO14S-120-0000 -0200 . . . . . . . . . . . .30 CO14S-230-0000 -0200 . . . . . . . . . . . .30 CO170R-120-0000 -1200 . . . . . . . . . . . .29 CO170R-230-0000 -1200 . . . . . . . . . . . .29 CO170S-120-0000 -1000 . . . . . . . . . . . .29 CO170S-230-0000 -1000 . . . . . . . . . . . .29 CO48R-120-0000 -1200 . . . . . . . . . . . .30 CO48R-230-0000 -1200 . . . . . . . . . . . .30 CO48S-120-0000 -1000 . . . . . . . . . . . .30 CO48S-230-0000 -1000 . . . . . . . . . . . .30 D100 . . . . . . . . . . . . . . . .25 D49 . . . . . . . . . . . . . . . . .25 D64 . . . . . . . . . . . . . . . . .25 D81 . . . . . . . . . . . . . . . . .25 K0200-0506 . . . . . . . . . . 23 K0200-0511 . . . . . . . . . . 23 K0200-0516 . . . . . . . . . . 23 K0280-1030-1031 . . . . . 25 K0280-1034 . . . . . . . . . . 25 K0280-1036 . . . . . . . . . . 25 K0440-0355 . . . . . . . . . . 25 K0440-0600 . . . . . . . . . . 25 K0540-0025 . . . . . . . . . . 25 K0641-1690 . . . . . . . . . . 26 K0641-1700 . . . . . . . . . . 26 K0641-1750 . . . . . . . . . . 26 K0641-1880 . . . . . . . . . . 26 K0641-1890 . . . . . . . . . . 26 K0641-1900 . . . . . . . . . . 26 K0641-3000-3005 . . . . . 26 K0660-0051 . . . . . . . . . . 25 K0641-1880 . . . . . . . . . . 26 M1001-0240 . . . . . . . 12, 18 M1001-9920 . . . . . . . 12,18 M1016-0890 . . . . . . . 57, 70 M1016-0900 . . . . . . . 57, 70 M1020-1220 . . . . . . . . . . 10 M1052-0700 . . . . . . . . . . 64 M1052-0760 . . . . . . . . . . 64 M1052-3170 . . . . . . . . . . 64 M1052-3180 . . . . . . . . . . 64 M1053-0306 . . . . . . . . . . 32 M1053-0360 . . . . . . . . . . 32 M1053-0450 . . . . . . . . . . 32 M1053-4004-4005 . . . . . 32 M1053-5004-5005 . . . . . 32 M1075-0100 . . . . . . . . . .33 M1075-0630 . . . . . . . . . .33 M1075-0640 . . . . . . . . . .33 M1117-2040 . . . . . . . . . . 49 M1131-8009 . . . . . . . . . . 70 M1131-8010 . . . . . . . . . . 69 M1153-9633 . . . . . . . . . . 63 M1153-9639 . . . . . . . . . . 63 M1154-2395-2396 . . . . . 64 M1171-8010 . . . . . . . . . . 66 M1176-0120 . . . . . . . . . . 57 M1176-0315 . . . . . . . . . . 57 M1176-0335 . . . . . . . . . . 57 M1176-0350 . . . . . . . . . . 57 M1176-1000-1008 . . . . . 57 M1176-1012-1013 . . . . . 57 M1176-1016 . . . . . . . . . . 57 M1176-1020 . . . . . . . . . . 57 M1176-1030 . . . . . . . . . . 57 M1176-5200 . . . . . . . . . . 57 M1176-9351-9352 . . . . . 57 M1176-9540 . . . . . . . 57, 70 M1176-9575 . . . . . . . 57, 70 M1176-9579 . . . . . . . 57, 70 M1190-0000-0003 . . . . . 11 M1190-0010-0013 . . . . . 11 M1190-0300 . . . . . . . . . . 12 M1190-6100 . . . . . . . . . . 12 M1190-9000-9005 . . . . . . 5 M1190-9900-9911 . . . . . 12 M1190-9915 . . . . . . . 12, 18 M1190-9916 . . . . . . . 12, 18 M1190-9917 . . . . . . . 12, 18 M1190-9918 . . . . . . . 12, 18 M1190-9919 . . . . . . . 12, 18 M1190-9920 . . . . . . . . . . 12 M1191-0000-0003 . . . . . 11 M1191-0010-0013 . . . . . 11 M1191-0020-0023 . . . . . 11 M1191-0030-0033 . . . . . 11 M1191-6102 . . . . . . . . . . 13 M1191-9904-9905 . . . . . 13 M1191-9908 . . . . . . . 13, 17 M1191-9909 . . . . . . . 13, 17 M1191-9910 . . . . . . . 13, 17 M1191-9911 . . . . . . . 13, 17 M1191-9912-9914 . . . . . 13 M1192-6002 . . . . . . . . . . . 9 M1192-9901 . . . . . 9, 12, 16 M1194-0000-0003 . . . . . 11 M1194-0010-0013 . . . . . 11 M1194-6001 . . . . . . . 12, 13 M1194-9903 . .9, 12, 16, 18 M1194-9904 . . 9, 12, 16, 18 M1194-9905 . . 9, 12, 16, 18 M1194-9906 . . 9, 12, 16, 18 M1194-9907 . . 9, 12, 16, 18 M1194-9908 . . 9, 12, 16, 18 M1194-9909 . . 9, 12, 16, 18 M1194-9910 . . 9, 12, 16, 18 M1194-9912 . . . . . . . . . . 13 M1194-9915-9923 . . . . . 12 M1194-9923 . . . . .9, 16, 18 M1194-9924 . . . . . . . 12, 13 M1194-9925 . . . . . . . . . . 13 M1194-9926 . . . . . . . . . . 12 M1194-9927 . . . . . . . . . . 13 M1195-1020 . . . . . . . 10, 19 M1196-0000 . . . . . . . . . . 14 M1196-0001-0003 . . . . . 14 M1196-6000 . . . . . . . . . . 14 M1196-9447 . . . . . . . . . . 14 M1197-9900-9907 . . . . . 14 M1197-2500 . . . . . . . . . . . . M1224-0001-0002 . . . . . 33 M1224-0005-0006 . . . . . 33 M1224-0100 . . . . . . . . . . 33 M1224-0110 . . . . . . . . . . 33 M1224-0160 . . . . . . . . . . 33 M1224-3000 . . . . . . . . . . 33 M1224-9200 . . . . . . . . . . 33 M1224-9740 . . . . . . . . . . 33 M1226-9446 . . . . . . . 57, 70 M1227-9231 . . . . . . . . . . 51 M1227-9935 . . . . . . . . . . 64 M1228-0020-0023 . . . . . 57 M1228-0120-0122 . . . . . 57 M1228-0310 . . . . . . . . . . 57 M1228-1012-1015 . . . . . 57 M1228-3002 . . . . . . . . . . 57 M1228-3005 . . . . . . . . . . 57 M1228-3007 . . . . . . . . . . 57 M1228-3012 . . . . . . . . . . 57 M1228-3014-3015 . . . . . 57 M1228-3017 . . . . . . . . . . 57 M1228-3024 . . . . . . . . . . 57 M1228-5014 . . . . . . . 41, 64 M1228-5016 . . . . . . . . . . 64 M1228-5026-5029 . . . . . 57 M1228-5200-5203 . . . . . 57 M1228-8001 . . . . . . . . . . 63 M1228-9209 . . . . . . . . . . 57 M1228-9401 . . . . . . . . . . 57 M1228-9403-9404 . . . . . 57 M1228-9409 . . . . . . . . . . 57 M1230-3030 . . . . . . . . . . 49 M1230-9212 . . . . . . . . . . 49 M1231-0000-0003 . . . . . 10 M1231-0730 . . . . . . . . . . 10 M1231-2000 . . . . . . . 10, 19 M1231-2010 . . . . . . . 10, 19 M1231-6000 . . . . . . . 10, 19 M1231-9920 . . . . . . . 10, 19 M1231-9930 . . . . . . . 10, 19 M1231-9933 . . . . . . . 10, 19 M1231-9934 . . . . . . . 10, 19 M1231-9935 . . . . . . . 10, 19 M1231-9936 . . . . . . . 10, 19 M1231-9937 . . . . . . . 10, 19 M1231-9938 . . . . . . . 10, 19 M1231-9939 . . . . . . . 10, 19 M1232-0100-0101 . . . . . 14 M1232-0110-0111 . . . . . 14 M1233-6002 . . . . . . . . . . . 8 M1233-9930 . . . . . . . . . . . 9 M1233-9932 . . . . .9, 12, 16 M1233-9940-9946 . . . . . . 8 M1233-9992 . . . . . . . . . . . 8 M1233-9996 . . . . . . . . . . . 8 M1238-8001 . . . . . . . . . . 69 M1238-8002 . . . . . . . . . . 70 M1240-1000 . . . . . . . . . . 33 M1243-2500 . . . . . . . .7, 17 M1248-0002-0003 . . . . . 19 M1248-0730 . . . . . . . . . . 19 M1250-9504 . . . . . . . . . . . 5 M1250-9600 . . . . . . . . . . . 5 M1250-9700 . . . . . . . . . . . 5 M1250-9902 . . . . 12, 16, 18 M1250-9902 . . . . . . . . . . . 9 M1250-9903 . .9, 12, 16, 18 M1250-9904 . . . . .7, 13, 17 M1250-9906 . .9, 12, 16, 18 M1250-9920 . . . . .7, 13, 17 M1250-9921 . . . . . . . 13, 17 M1266-0000-0001 . . . . . 65 M1273-1001-1002 . . . . . 47 M1273-1005 . . . . . . . . . . 47 M1273-1010-1012 . . . . . 47 M1273-1015 . . . . . . . . . . 47 M1273-1020 . . . . . . . . . . 47 M1273-1021-1022 . . . . . 48 M1273-1025 . . . . . . . . . . 48 M1273-1030-1032 . . . . . 48 M1273-1035 . . . . . . . . . . 48 M1273-1040-1042 . . . . . 48 M1273-1045 . . . . . . . . . . 48 M1273-1050-1052 . . . . . 48 M1273-1055 . . . . . . . . . . 48 M1273-1060 . . . . . . . . . . 48 M1273-1101-1102 . . . . . 47 M1273-1105 . . . . . . . . . . 47 M1273-1110-1112 . . . . .47 M1273-1115 . . . . . . . . . . 47 M1273-1120-1122 . . . . . 47 M1273-1125 . . . . . . . . . . 47 M1273-1130 . . . . . . . . . . 47 M1273-1140-1142 . . . . . 47 M1273-1145 . . . . . . . . . . 47 M1273-1150-1152 . . . . . 47 M1273-1155 . . . . . . . . . . 47 M1273-1160-1162 . . . . . 47 M1273-1165 . . . . . . . . . . 47 M1273-1170-1172 . . . . . 48 M1273-1175 . . . . . . . . . . 48 M1273-1201-1202 . . . . . 48 M1273-1205 . . . . . . . . . . 48 M1273-1210-1212 . . . . . 48 M1273-1215 . . . . . . . . . . 48 M1273-1220 . . . . . . . . . . 48 M1273-1301 . . . . . . . . . . 48 M1273-1302 . . . . . . . . . . 48 M1273-1305 . . . . . . . . . . 48 M1273-1310-1312 . . . . . 48 M1273-1315 . . . . . . . . . . 48 M1273-1320 . . . . . . . . . . 48 M1273-1370-1372 . . . . . 48 M1273-1375 . . . . . . . . . . 48 M1273-1401-1402 . . . . . 48 M1273-1405 . . . . . . . . . . 48 M1273-1410 . . . . . . . . . . 48 M1273-1501-1502 . . . . . 48 M1273-1505 . . . . . . . . . . 48 M1273-1510 . . . . . . . . . . 48 M1273-1601-1602 . . . . . 47 M1273-1605 . . . . . . . . . . 47 M1273-1610-1612 . . . . . 47 M1273-1615 . . . . . . . . . . 47 M1273-1620-1622 . . . . . 47 M1273-1625 . . . . . . . . . . 47 M1273-1630-1632 . . . . . 47 M1273-1635 . . . . . . . . . . 47 M1273-1640 . . . . . . . . . . 47 M1273-3031 . . . . . . . . . . 49 M1273-3100-3112 . . . . . 48 M1273-3116 . . . . . . . . . . 48 M1273-3120 . . . . . . . . . . 48 M1273-3125 . . . . . . . . . . 48 M1273-3130 . . . . . . . . . . 48 M1273-3135 . . . . . . . . . . 48 M1273-3201-3202 . . . . . 49 M1273-3205 . . . . . . . . . . 49 M1273-3210-3212 . . . . . 49 M1273-3215 . . . . . . . . . . 49 M1273-3220 . . . . . . . . . . 49 M1273-5001 . . . . . . . 49, 51 M1273-5001 . . . . . . . 56, 58 M1273-5002 .49, 51, 56, 58 M1273-5003-5007 . . . . . 49 M1273-5040 . . . . . . . . . . 49 M1273-5042 . . . . . . . . . . 49 M1273-5054 . . . . . . . . . . 49 M1273-5056 . . . . . . . . . . 49 M1273-5058 . . . . . . . . . . 49 M1273-5062-5064 . . . . . 49 M1273-5065 . . . . . . . . . . 49 M1273-5070-5074 . . . . . 49 M1273-9206 . . . . . . . 49, 58 M1273-9207 . . . . . . . . . . 49 M1273-9266 . . . . . . . . . . 49 M1273-9290 . . . . . . . 49, 58 M1273-9291-9293 . . . . . 49 M1273-9405-9407 . . . . . 49 M1273-9575 . . . . . . . . . . 49 M1273-9850-9853 . . . . . 48 M1273-9900-9902 . . . . . 49 M1273-9907-9909 . . . . . 49 M1273-9915-9919 . . . . . 49 M1273-9930-9933 . . . . . 48 M1273-9936 . . . . . . . . . . 49 M1273-9940 . . . . . . . . . . 49 M1273-9942 . . . . . . . . . . 49 M1273-9945-9947 . . . . . 49 M1273-9951 . . . . . . . . . . 49

1.732.287.1200 · 1.800.631.5417 · Fax: 1.732.287.4222 · [email protected]

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Index by Catalog Number

NBS Part No. Page NBS Part No. Page M1273-9953 . . . . . . . . . . 49 M1273-9956-9957 . . . . . 49 M1273-9960-9961 . . . . . 49 M1273-9970-9979 . . . . . 48 M1273-9982 . . . . . . . . . . 48 M1273-9985-9986 . . . . . 48 M1273-9988 . . . . . . . . . . 48 M1273-9989 . . . . . . . 49, 51 M1273-9990 . . . . . . . 49, 51 M1273-9991-9992 . . . . . 49 M1273-9998-9999 . . . . . 49 M1276-0000 . . . . . . . . . . 65 M1276-5000 . . . . . . . . . . 65 M1282-0000-0007 . . . . . . 6 M1282-0010-0017 . . . . . . 6 M1282-0291 . . . . . . . . . . . 6 M1282-0500-0501 . . . . . . 6 M1282-0600 . . . . . . . . . . . 6 M1282-0700 . . . . . . . . . . . 6 M1282-0800 . . . . . . . . . . . 6 M1282-5011 . . . . . . . . . . . 6 M1282-6000 . . . . . . . . . 6, 7 M1282-8038 . . . . . . . . . . . 6 M1282-9904-9911 . . . . . . 6 M1282-9913 . . . . . . . . . . . 6 M1282-9915 . . . . . . . . . . . 6 M1282-9921-9922 . . . . . . 6 M1282-9925-9928 . . . . . . 6 M1286-0000 . . . . . . . . . . 66 M1286-0100 . . . . . . . . . . 66 M1286-8010 . . . . . . . . . . 66 M1287-0020 . . . . . . . 51, 56 M1287-0101-0102 . . . . . 51 M1287-0111 . . . . . . . . . . 56 M1287-0114 . . . . . . . . . . 56 M1287-0300-0303 . . . . . 50 M1287-0310-0313 . . . . . 55 M1287-0320-0323 . . . . . 50 M1287-0330-0333 . . . . . 55 M1287-0400 . . . . . . . 50, 55 M1287-0401 . . . . . . . 50, 55 M1287-0402 . . . . . . . 50, 55 M1287-0403 . . . . . . . 50, 55 M1287-0410-0413 . . . . . 50 M1287-0674-0676 . . . . . 55 M1287-1002-1005 . . . . . 51 M1287-1010 . . . . . . . 50, 55 M1287-1020 . . . . . . . 50, 55 M1287-1110-1113 . . . . . 56 M1287-1125-1128 . . . . . 56 M1287-1135-1143 . . . . . 56 M1287-1150 . . . . . . . . . . 56 M1287-1170-1177 . . . . . 50 M1287-1185-1193 . . . . . 56 M1287-1200-1208 . . . . . 51 M1287-1210-1213 . . . . . 55 M1287-1260-1267 . . . . . 54 M1287-2010-2019 . . . . . .55 M1287-2021 . . . . . . . . . . 55 M1287-2110-2112 . . 50, 55 M1287-3500 . . . . . . . . . . 56 M1287-3501 . . . . . . . . . . 51 M1287-3505 . . . . . . . . . . 56 M1287-3510 . . . . . . 50, 55 M1287-3520 . . . . . . . 50, 55 M1287-3530 . . . . . . . 51, 56 M1287-3540 . . . . . . . . . . 50 M1287-3550 . . . . . . . . . . 56 M1287-5010-5013 . . . . . 56 M1287-5030-5031 . . . . . 51 M1287-5034 . . . . . . . . . . 51 M1287-5042 . . . . . . . 51, 56 M1287-5043 . . . . . . . . . . 51 M1287-5068-5073 . . . . . 56 M1287-5074-5075 . . . . . 56 M1287-6020 . . . . . . . . . . 51 M1287-6021-6021 . . . . . 56 M1287-6022-6023 . . . . . 51 M1287-6030-6033 . . . . . 56 M1287-9212 . . . . . . . . . . 51 M1287-9227-9230 . . . . . 56 M1287-9287-9290 . . . . . 56 M1287-9914-9915 . . . . . 51 M1287-9918 . . . . . . . . . . 63 M1287-9920-9923 . . . . . 56 M1287-9930-9933 . . . . . 51 M1287-9950-9955 . . . . . 56 M1287-9964 . . . . . . . . . . 51 M1287-9966 . . . . 51, 56, 63 M1287-9967 . . . . . . . 51, 56 M1287-9985-9986 . . . . . 56 M1287-9987 . . . . . . . 56, 63 M1287-9988 . . . . . . . . . . 56 M1289-0001-0006 . . . . . . 5 M1289-0010 . . . . . . . . . . . 5 M1289-0020 . . . . . . . . . . . 5 M1289-0030 . . . . . . . . . . . 5 M1289-0040 . . . . . . . . . . . 5 M1289-0050 . . . . . . . . . . . 5 M1289-0060 . . . . . . . . . . . 5 M1289-0100 . . . . . . . . . . . 5 M1289-0200 . . . . . . . . . . . 5 M1289-0300 . . . . . . . . . . . 5 M1289-0400 . . . . . . . . . . . 5 M1289-0500 . . . . . . . . . . . 5 M1289-0600 . . . . . . . . . . . 5 M1289-0700 . . . . . . . . . . . 5 M1290-0610 . . . . . . . 68, 69 M1290-8012 . . . . . . . 68, 69 M1291-0000 . . . . . . . . . . 66 M1291-0010-0011 . . . . . 66 M1291-0054 . . . . . . . . . .32 M1291-8021 . . . . . . . . . .32 M1291-8022 . . . . . . . . . .32 M1291-8023 . . . . . . . . . .32 M1292-0020 . . . . . . . . . . 42 M1292-0100 . . . . . . . . . . 42 M1292-1000 . . . . . . . . . . 42 M1292-9984 . . . . . . . 40, 57 M1292-9988 . . . . . . . 40, 57 M1293-0000 . . . . . . . . . . 42 M1293-0020 . . . . . . . . . . 42 M1293-0100 . . . . . . . . . . 42 M1293-1000 . . . . . . . . . . 42 M1293-0900 . . . . . . . . . . 42 M1294-5013 . . . . . . . . . . 58 M1294-5023 . . . . . . . . . . 58 M1294-9542 . . . . . . . . . . 58 M1294-9544 . . . . . . . . . . 58 M1294-9820 . . . . . . . . . . 58 M1294-9822 . . . . . . . . . . 58 M1294-9824 . . . . . . . . . . 58 M1294-9826 . . . . . . . . . . 58 M1294-9828 . . . . . . . . . . 58 M1294-9830 . . . . . . . . . . 58 M1294-9836 . . . . . . . . . . 58 M1294-9940-9945 . . . . . 58 M1294-9948 . . . . . . . . . . 58 M1294-9954 . . . . . . . . . . 58 M1295-0000-0001 . . . . . 65 M1299-0061 . . . . . . . . . . . 9 M1299-0080-0087 . . . . . . 9 M1299-0090-0097 . . . . . . 9 M1299-6000 . . . . . . . . . . . 9 M1320-0000-0007 . . . . . . 7 M1320-0010-0017 . . . . . . 7 M1320-0300 . . . . . . . . . . . 7 M1320-0400 . . . . . . . . . . . 7 M1320-0500 . . . . . . . . . . . 7 M1320-0600 . . . . . . . . . . . 7 M1320-0700 . . . . . . . . . . . 7 M1320-6000 . . . . . . . . . . . 7 M1320-8029 . . . . . . . 7, 8, 9 M1322-0000 . . . . . . . . . . 25 M1322-0002 . . . . . . . . . . 25 M1324-0000-0007 . . . . . 15 M1324-0500 . . . . . . . . . . 15 M1324-0600 . . . . . . . . . . 15 M1324-0700 . . . . . . . . . . 15 M1324-0800 . . . . . . . . . . 15 M1324-9904-9911 . . . . . 15 M1325-0000 . . . . . . . . . . 33 M1325-0002 . . . . . . . . . . 33 M1325-2210 . . . . . . . . . . 33 M1327-1000-1001 . . . . . 41 M1327-9980-9981 . . . . . 41 NBS Part No. Page M1335-0000-0007 . . . . . . 8 M1335-0010-0017 . . . . . . 8 M1335-0080 . . . . . . . . . . . 8 M1335-0300 . . . . . . . . . . . 8 M1335-0400 . . . . . . . . . . . 8 M1335-0500-0501 . . . . . . 8 M1335-0600 . . . . . . . . . . . 8 M1335-0800 . . . . . . . . . . . 8 M1335-6000 . . . . . . . . . . . 8 M1350-0000-0001 . . . . . 18 M1351-0000-0001 . . . . . 17 M1352-0000-0007 . . . . . 16 M1352-6000 . . . . . . . . . . 16 M1353-0000-0007 . . . . . 17 M1354-0000-0001 . . . . . 18 M1355-0000-0001 . . . . . 18 M1360-0101-0103 . . . . . 58 M1360-1120-1121 . . . . . 58 M1360-1123 . . . . . . . . . . 58 M1360-2020-2021 . . . . . 58 M1360-2023 . . . . . . . . . . 58 M1360-2030-2038 . . . . . 58 M1360-2040-2041 . . . . . 58 M1360-2050 . . . . . . . . . . 58 M1360-9832 . . . . . . . . . . 58 M1360-9834 . . . . . . . . . . 58 M1360-9969 . . . . . . . . . . 58 M1361-9208 . . . . 68, 69, 70 P0740-2730 . . . . . . . . . . 41 P0160-4440 . . . . . . . . . . 33 P0160-4460 . . . . . . . . . . 49 P0200-2050 . . . . . . . . . . 57 P0200-2227 . . . . . . . . . . 57 P0200-2367 . . . . . . . . . . 57 P0200-2410 . . . . . . . . . . 57 P0200-2427 . . . . . . . . . . 57 P0200-2600 . . . . . . . . . . 57 P0240-1080 . . . . . . . . . . 63 P0240-1090 . . . . . . . . . . 63 P0240-2670 . . . . . . . . . . 49 P0240-2680 . . . . . . . . . . 49 P0240-5000 . . . . . . . . . . 63 P0280-2582 . . . . . . . . . . 64 P0300-0350 . . . . . . . . . . 33 P0381-0380 . . . . . . . . . . 69 P0440-0061A . . . . . . . . . 63 P0440-0064 . . . . . . . . . . 58 P0440-0500 . . . . . . . . . . 41 P0440-0508 . . . . . . . . . . 41 P0440-4321 . . . . . . . 51, 56 P0440-4327 . . . . . . . 51, 56 P0440-4489 . . . . . . . 51, 56 P0460-7550 . . . . . . . . . . 66 P0540-1082 . . . . . . . . . . 64 P0540-5440 . . . . . . . . . . 42 P0620-0653-0654 . . . . . 63 P0620-0662 . . . . . . . . . . 63 P0620-0664 . . . . . . . . . . 63 P0620-0920-0921 . . . . . 63 P0620-0959 . . . . . . . . . . 63 P0620-2410A . . . . . . . . . 63 P0620-2411A . . . . . . . . . 63 P0620-2796 . . . . . . . . . . 63 P0620-2798-2799 . . . . . 63 P0620-3040 . . . . . . . . . . 41 P0620-5192-5193 . . . . . 70 P0620-5551 . . . . . . . . . . 68 P0620-5580 . . . . . . . . . . 70 P0620-5950 . . . . . . . . . . 25 P0620-5960 . . . . . . . . . . 25 P0620-5974-5975 . . . . . 68 P0620-6430 . . . . . . . . . . 33 P0620-6460 . . . . . . . . . . 41 P0620-6470 . . . . . . . . . . 41 P0620-6580 . . . . . . . . . . 68 P0625-0020 . . . . . . . . . . 23 P0625-1090 . . . . . . . . . . 23 P0625-1110 . . . . . . . . . . 23 P0625-1170 . . . . . . . . . . 25 P0625-1180 . . . . . . . . . . 25 P0625-1630 . . . . . . . . . . .27 P0625-1635 . . . . . . . . . . .27 P0625-1640 . . . . . . . . . . .27 NBS Part No. Page P0625-1650 . . . . . . . . . . .27 P0625-1651 . . . . . . . . . . .27 P0625-1652 . . . . . . . . . . .27 P0626-0010 . . . . . . . . . . 33 P0626-0020 . . . . . . . . . . 33 P0626-0030 . . . . . . . . . . 33 P0626-0040 . . . . . . . . . . 33 P0626-0050 . . . . . . . . . . 33 P0626-0060 . . . . . . . . . . 33 P0626-0070 . . . . . . . . . . 33 P0626-0080 . . . . . . . . . . 33 P0626-0090-0091 . . . . . 33 P0626-0100 . . . . . . . . . . 33 P0628-5000 . . . . . . . . . . .31 P0628-5020 . . . . . . . . . .31 P0628-5030 . . . . . . . . . .31 P0628-5040 . . . . . . . . . .31 P0628-5050 . . . . . . . . . .31 P0628-5060 . . . . . . . . . .31 P0628-5080 . . . . . . . . . .31 P0628-5090 . . . . . . . . . .31 P0628-5100 . . . . . . . . . .31 P0628-5260 . . . . . . . . . .31 P0628-5340 . . . . . . . . . .31 P0628-5350 . . . . . . . . . .31 P0628-5400 . . . . . . . . . .31 P0628-5410 . . . . . . . . . .31 P0628-5430 . . . . . . . . . .31 P0628-5540 . . . . . . . . . .31 P0628-5560 . . . . . . . . . .31 P0628-5600 . . . . . . . . . .31 P0628-5612 . . . . . . . . . .31 P0628-5790 . . . . . . . . . .31 P0628-5940 . . . . . . . . . .31 P0628-6150 . . . . . . . . . .31 P0628-6160 . . . . . . . . . .31 P0628-6170 . . . . . . . . . .31 P0628-6200 . . . . . . . . . .31 P0628-6210 . . . . . . . . . .31 P0628-6230 . . . . . . . . . .31 P0628-6251 . . . . . . . . . .31 P0628-6260 . . . . . . . . . .31 P0628-6270 . . . . . . . . . .31 P0628-6280 . . . . . . . . . .31 P0628-6300 . . . . . . . . . .31 P0628-6400 . . . . . . . . . .31 P0628-6760 . . . . . . . . . .31 P0628-6761 . . . . . . . . . .31 P0628-6770 . . . . . . . . . .31 P0628-6780 . . . . . . . . . .31 P0628-6781 . . . . . . . . . .31 P0628-6790 . . . . . . . . . .31 P0628-6800 . . . . . . . . . .31 P0628-6810 . . . . . . . . . .31 P0628-6820 . . . . . . . . . .31 P0640-0420 . . . . . . . . . . . .69 P0640-0470-0471. . . . . . . 64 P0640-0480 . . . . . . . . . . 64 P0640-0500 . . . . . . . . . . 64 P0640-0518 . . . . . . . . . . 41 P0640-0600 . . . . . . . . . . 41 P0640-0610 . . . . . . . . . . 41 P0640-0620 . . . . . . . . . . 41 P0640-8060C . . . . . . . . . 64 P0640-8070C . . . . . . . . . 64 P0660-0106 . . . . . . . . . . 64 P0720-2090 . . . . . . . . . . 69 P0720-2093-2095 . . . . . 69 P0720-2273 . . . . . . . . . . 69 P0720-2275-2277 . . . . . 68 P0720-2300 . . . . . . . . . . 70 P0720-2331-2333 . . . . . 70 P0720-2336 . . . . . . . . . . 70 P0720-2342 . . . . . . . . . . .70 P0720-2430 . . . . . . . . . . 68 P0720-2560-2561 . . . . . 70 P0720-2763 . . . . . . . . . . 68 P0720-5317 . . . . . . . . . . 69 P0720-5567 . . . . . . . . . . 70 P0720-5570 . . . . . . . .69, 70 P0720-5570C . . . 68, 69, 70 P0720-5580-5584 . . . . . 69 P0720-5631 . . . . . . . 69, 70 NBS Part No. Page P0720-5750 . . . . . . . 69, 70 P0720-5780-5782 . . . . . 68 P0720-5784 . . . . . . . . . . 68 P0720-5950-5951 . . . . . 68 P0720-5960-5963 . . . . . 68 P0720-6240 . . . . . . . 69, 70 P0720-6240C3 . . 68, 69, 70 P0720-6250 . . . . . . . . . . 69 P0720-6268 . . . . . . . . . . 70 P0720-6270 . . . . . . . . . . 70 P0720-6280-6283 . . . . . 70 P0720-6284 . . . . . . . . . . 70 P0720-6339 . . . . . . . . . . 70 P0720-6450C1 . . 68, 69, 70 P0720-6470 . . . . . . . . . . 70 P0720-6480-6482 . . . . . 68 P0720-6520 . . . . . . . . . . 70 P0720-6530-6532 . . . . . 68 P0720-6540 . . . . . . . . . . 69 P0720-6570 . . . . . . . . . . 58 P0720-9660 . . . . . . . . . . 68 P0740-2396 . . . . . . . . . . 63 P0740-2430 . . . . . . . . . . 63 P0740-2445 . . . . . . . . . . 63 P0740-2505 . . . . . . . 33, 63 P0740-2542 . . . . . . . . . . 63 P0740-2590 . . . . . . . . . . 63 P0740-2721C3 . . . . . . . . 63 P0740-2730 . . . . . . . . . . 41 P0740-2740 . . . . . . . . . . 41 P0740-3110 . . . . . . . . . . 63 P0740-3111C3 . . . . . . . . 63 P0740-3113C3 . . . . . . . . 63 P0820-5179 . . . . . . . . . . 42 P0820-5180 . . . . . . . . . . 42 P0820-5185 . . . . . . . . . . 42 P0820-5190 . . . . . . . . . . 42 P0820-5200 . . . . . . . . . . 42 P0860-0440 . . . . . . . . . . 69 P0860-1050 . . . . . . . . . . 49 P0860-5178 . . . . . . . . . . 42 PTL-386 . . . . . . . . . . . . . 13 PTL-393 . . . . . . . . . . . . . 13 S2116-3051P . . . . . . . . . . 5 S2116-3101P . . . . . . . . . . 5 TTR-210 . . . . . . . . . . . . . . 5 TTR-215 . . . . . . . . . . . . . . 5 TTR-221 . . . . . . . . . . . . . . 5 U9043-0002 . . . . . . . . . . 25 U9043-0004 . . . . . . . . . . 25 U9043-0008 . . . . . . . . . . 25 U9044-0002 . . . . . . . . . . 25 U9044-0004 . . . . . . . . . . 25 U9045-0000-0001 . . . . . 25 U9230-0000-0001 . . . . . 24 U9250-0001-0002 . . . . . 24 U9260-0000-0002 . . . . . 24 U9270-0000-0002 . . . . . 24 U9270-0004-0005 . . . . . 21 U9400-0000-0002 . . . . . 22 U9410-0001-0002 . . . . . 22 U9420-0000-0001 . . . . . 23 U9425-0000-0001 . . . . . 23 U9430-0000-0002 . . . . . 22 U9440-0001-0002 . . . . . 22 U9440-0004-0005 . . . . . 21 XAR-100 . . . . . . . . . . . . . 64 XMF-8624 . . . . . . . . . . . . 51

74

New Brunswick Scientific · a culture of innovation · www.nbsc.com

NBS -86°C Innova Freezers.

More Storage. Same Space.

Room You Won't Have to Make Room For.

· Space-Saving Vacuum Insulation Panels outperform traditional insulating foams AND provide up to 30% more room in the same space. · New Eco-Friendly "Green" Models are the most energy-efficient & environmentallyfriendly freezers on the market. · Unique, Upright, On-or-Under Bench Innova® U101 freezers provide the convenience & security of a personal-sized freezer for in-lab use. · Ultra-Slender Innova U360 Freezers Fit Where Other Freezers Can't. With an extra-large display, 72-hr. data storage, trend graphing & more. · Ten Models -- All Energy Efficient, Quiet Running and Dependable.

See pages 20 - 27 or download our brochure at www.nbsc.com

a culture of innovation

USA HEADQUARTERS Box 4005, 44 Talmadge Rd. Edison, NJ 08818-4005 1.800.631.5417 +1.732.287.1200 FAX: +1.732.287.4222 [email protected]

BELGIUM Stationsstraat 180/4 3110 Rotselaar +32 (0) 16 56 28 31 Fax: +32 (0) 16 57 27 53 [email protected]

THE NETHERLANDS Kerkenbos 1101, 6546 BC P.O. Box 6826, 6503 GH Nijmegen +31 (0)24 3717 600 Fax: +31 (0)24 3717 640 [email protected]

UNITED KINGDOM 17 Alban Park, Hatfield Road St. Albans, Hertfordshire AL4 0JJ Free Tel: 0800 581331 +44 (0) 1727 853 855 Fax: +44 (0) 1727 835 666 [email protected] www.nbsuk.co.uk

CHINA, Shanghai A903, 904 Yin Hai Building, No. 250, Cao Xi Road Shanghai 200235 +86 21 64845955, 64845966 Fax: +86 21 64845933 [email protected]

www.nbsc.com · [email protected]

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