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Issue 3




Spring 2009

The Georgia Society for Histotechnology


President's Message Coverslipping Article Memorial to Lyle Baker GSH Board of Directors GSH Meeting--Callaway Gardens GSH Membership Form NSH Program--Seattle NSH Membership Form

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Fall is Almost here ... and NSH is just around the corner... Get Ready for Seattle!

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President Letter....

September 2010

Dear GSH Members, On August 7, I officially retired from Piedmont Newnan Hospital, where I had worked for some 40 plus years. As I reflect back over that time, I can see a lot of faces and remember a lot of names of the great Histotechnologist ' s who made our profession what it is. I've spent a great deal of time with people like Lee Luna, Dominique Europa, Dezna Sheehan, Charlie Cullings, Lyle Baker and many others who fought so hard for the profession. They were people of honor, integrity and courage, who stood for the profession and made it what it is today. One of Lee Luna's favorite sayings was; " if you have time to do it

over; you had time to do it right. "

I hope that in my going, I left a legacy behind me. I always put pride in the job that I did, and treated every patient as if they were a close family member. I often told my students; " give no less than you would

expect to receive. " I'm never spent any time with a student that I did not promote the profession and

where I think it will be in 10 years. We are entering an interesting phase in the field as the numbers of active Histotechnologist are decreasing each day. I wish I had some magic formula or wand that I could wave and solve the problem, but I don't. It was with great sadness that I saw my job being posted and under requirement was listed MLT or MT. HT ( ASCP ) was mentioned, but not stressed. I hope that a good Histotechnologist does apply for and gets the job. It is truly a great place to work, with two wonderful Pathologists. It is my intention to continue to serve the Georgia Society for Histotechnology in the capacity as your president. I encourage your active participation, input, thoughts and ideas. Chris and I have signed the contract for next year ' s meeting which will be March 25 - 27, 2011 at Callaway Gardens in Pine Mountain. The room rates will be $99 to include a continental breakfast and admission to the garden and its amenities. The rooms have been renovated and are absolutely gorgeous. For those of you who came the last time, the meeting rooms will be in the same area, but the rooms will be at the top near the meeting rooms. I want to encourage you to attend and participate in a great meeting. The last time we were there we had 115 people, so get your reservations early. I hope you have a great fall, and we will see you at Callaway Gardens. Mike Ayers President GSH

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Do You Remember the Cities Where GSH was Held?

DATE March 4, 1972 March 4, 1973 July 26-27, 1973 Sept. 27, 1974 July 18-20, 1975 July 16-18, 1976 July 20-24, 1977 June 17, 1978 June 23, 1979 Oct. 27-31, 1980 May 22-23, 1981 May 28-29, 1982 June 23-25, 1983 June 7-9, 1984 March 28-30, 1985 May 23-24, 1986 May 15-16, 1987 May 20-21, 1988 May 18-20, 1989 May 17-19, 1990 May 16-18, 1991 April 31-May 2, 1992 March 24-27, 1993 May 5-7, 1994 May 18-20, 1995 May 2-6, 1996 May 29-31, 1997 May 7-9, 1998 March 11-13, 1999 May 20-22, 2000 April 27-28, 2001 May 17-18, 2002 April 11-12, 2003 April 2-4, 2004 March 5-6, 2005 June 3, 2006 April 13-15, 2007 April 3-5, 2008 March 20-22, 2009 March 26-28, 2010 March 25-27, 2011 LOCATION Ramada Inn Palymra Park Hospital Buccaneer Motorlodge Buccaneer Motorlodge Buccaneer Motorlodge Ramada Inn, Midtown Ramada Inn, Airport Hilton Hotel Colony Square Hotel Colony Square Hotel Sheraton-By-the-Sea Sheraton Inn and Country Club Lake Arrowhead Resort UGA Center for Continuing Education Terrace Garden Hyatt Regency Hotel Tower Place Unicoi St. Park Resort Holiday Inn at Arkwright Villas-By-the-Sea Holiday Inn, Buckhead Radisson Hotel Embassy Suites Villas-By-the-Sea Falcon Inn Radisson Riverfront Hotel Marriott Riverfront Hotel UGA Center for Continuing Education Marriott North Central UGA Center for Continuing Education Partridge Inn Holiday Inn Macon Conference Center Hyatt Regency Suites Perimeter NW Sea Palms Golf & Tennis Resort Hyatt Regency Suites Perimeter NW Marriott Augusta Hotel & Suites Mountain Creek Inn Westin Peachtree Plaza, Sea Palms Golf & Tennis Resort Evergreen Marriott Convention Resort Mountain Creek Inn CITY Macon Albany Jekyll Island Jekyll Island Jekyll Island Atlanta Atlanta Macon Atlanta Atlanta (NSH Symposium/Convention) Jekyll Island Savannah Waleska Athens Atlanta Savannah Atlanta Helen Macon Jekyll Island Atlanta Augusta Buckhead (Region III NSH) Jekyll Island Suwanee Augusta Savannah Athens Atlanta Athens Augusta Macon Marietta St. Simons Marietta Augusta Callaway Gardens Atlanta (Region III NSH) St. Simons St Mountain Callaway Gardens (Region III NSH)

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Coverslipping Productivity

by René J. Buesa, HTL (ASCP) (Ret.)

After a section has been stained, it is dehydrated and cleared, the next step being to cover it for its long term preservation. This step is called coverslipping and requires using a liquid medium that will dry out adhering a solid and flat surface over the stained section. Coverslipping can be manual or automated. There are two basic manual coverslipping techniques: one is to add the mounting medium over the slide laid flat over the counter, applying the coverslip over it. The other way is to apply the mounting medium over the coverslip pressing the slide with the section over it. In both ways the mounting medium has to be liquid enough as to cover the section leaving no air bubbles. The excess medium overflowing the limits of the coverslip has to be wiped off. Nowadays most mounting media are artificial and dry quickly, but many years ago, when Canada balsam was widely used, the coverslipped slides had to be left flat for days until the balsam dried out. Small lead fishing weights or small bottles with lead shots were sometimes placed over the coverslips to help drying; also the slides were placed in convection ovens. Faster drying was obtained using xylene diluted Dammar resin, but presently there are numerous synthetic mounting media that can be given the desired fluidity by adding either toluene or xylene. The ideal fluidity is that of low grade mineral oil. Any correctly diluted synthetic mounting medium can be dried within 24 hours and allow the finished slides to be stored properly. Manual coverslipping is less frequent in the US (about of histolabs) than in other countries where at least half of all histolabs coverslip manually. Manual coverslipping takes an average of 35 seconds per slide in the US, and 47 seconds in other countries, which is a statistically significant difference (P<0.05). Automated coverslippers can use a plastic film or glass coverslips. Film coverslippers use xylene to adhere the acetate based film to the section. Those using glass add the mounting medium to the section and press the coverslip over it mimicking the manual sequence. Automated coverslipping is less frequent than automated staining and the time it takes to cover one section is 3 to 5 seconds for film coverslippers, and 10 to 20 seconds with glass coverslippers. Film coverslipping has the productivity advantage but has two technical disadvantages. One, less important, refers to the preference by some pathologists who usually favor glass over film when taking photomicrographs, although film has proven to be adequate enough. The second is much more important and is caused by coverslippers delivering less xylene than needed due to calibration problems.

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This seemingly small problem will determine that some archival slides may present film detachment that usually ruins the slide by pulling the section attached to the film. All these factors have to be weighed when deciding which type of coverslip to buy. In the US 83% of histolabs with automated H&E stainers also have automated coverslippers, but in other countries about of histolabs with H&E autostainers coverslip manually. Coverslipping productivity differs between countries as summarized in the following Table. Type of Histology Lab and (Number of Each) % of Labs with Coverslippers 43 65 65 47 0 69 6 100 60 37 Productivity (Slides/ Hour) Manual 80 103 78 69 99 70 65 -- 75 61 Automated 150 143 120 160 -- 120 125 175 120 145

Total Human Pathology Histo Labs (379) US Labs (159) (a) Commonwealth of Nations (14) (b) Other Nations (13) Russian Histolabs (13) Spanish Labs (148) (c) Hispano-American Histolabs (32) (d) Reference Histology Labs (5) US--GI, Small and Renal Bx (5) (e) Veterinary Pathology Histology Labs

(a) Australia (3); Canada (6); New Zealand (1); South Africa (3); UK (1) (b) Austria (2); China (6); Dubai (2); Philippines (1); Poland (1); Saudi Arabia (1) (c) Argentina (4); Colombia (7); Ecuador (3); Mexico (14); Venezuela (4) (d) US (4); Venezuela (1) (e) Australia (2); UK (1); US (9)

Buesa RJ: Productivity standards for histology laboratories Annal Diag.Pathol., (2010) 14(2):107-124


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Join the contest for the best theme for the GSH Meeting! awarding $100.00 for best the best theme Mike Ayers and Chris Coley - judges



JUNE 21, 1937 -- JULY 16, 2010

Lyle Baker and many others who fought so hard for the profession, were people of honor, integrity and courage, who stood for the profession and made it what it is today. Friend and colleague Lyle Baker passed away July 16, 2010. Lyle had been active in both NSH and the North Carolina Society. A charter member of NSH, he served as Awards Chair from 1988 to 1996 and from 1992-2002. A native of Mahaska County, Iowa, Lyle was born on the longest day of the year, June 21, 1937, to Iowa farmers Howard and Hazel Maughan Baker. He was one of seven boys. He proudly wore a U.S. Navy uniform for 20 years, during which time he served in the Vietnam War as a Med Tech, and the on the USS Beale, a destroyer. He then assisted astronauts in Houston, Texas, as a Navy medic in a NASA test program entitled SMEAT, in 1973. After his retirement from the Navy, he continued his medical career by working for the Moses Cone Health System as a Chief Histotechnologist from 1973 until 1999. He then went on to work for Greensboro Pathology Associates until he finally retired in 2005. His life was certainly filled to the brim with happiness and love, spare time was spent in various ways; from establishing hospital histology labs to histology organizations, where he held multiple positions of authority. He led an amazing and fulfilling life. Lyle's achievements in Histology included Charter member of NSH, served as Awards Chair from 19982002. Lyle was NSH Representative to CLSI (formerly NCCLS) from 1986 to 1988. He was presented with the J.D. McCormick Award in 1997, Histotechnologist of the year in 2002 and the Lee G.Luna Foreign Travel Scholarship in 2005. Mequita Praet wrote: I was privileged to know Lyle Baker and so saddened to hear of his passing on July 16, 2010. Lyle was many things to me, friend, colleague, mentor, teacher and hero. I can't remember when I first met him. It seems like I have always known him. He asked me to be NSH Region III's secretary when he served as its Director, that cemented our friendship. From then on, I always looked forward to seeing him at the various meetings. I will always appreciate his leadership in our society and profession. He served NSH, Region III and NCSHT in many capacities with great honor, dedication, caring and wisdom. He was NSH Histotechnologist of the Year in 2002, an honor he richly deserved. Our future is dimmer without him. Goodbye, my friend, I'll always miss you. Mequita Praet, HTL (ASCP) SLS

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GSH PRESIDENT Mike Ayers Newnan, GA PH: 770- 304-4065 FX: 770- 253-2570 Email: [email protected]

HISTORIAN Janet Hobbs Augusta, GA PH: 706-721-8515 Email: [email protected]

GSH BYLAWS & LEGISLATIVE CHAIR Larry Fields Marietta, GA 803-917-4536 [email protected] MICROTIME EDITOR Carole Fields Marietta, GA PH: 404-895-9604 Email: [email protected]

GSH VICE PRESIDENT & GSH EXHIBIT LI-AISON Christopher M. Coley HT (ASCP) Acworth, GA Email: [email protected] (c) 706-506-5307

GSH TREASURER & BUDGET AND FINANCE CHAIR Mary Anne Taylor 6645 Goodall Mill Rd. Macon, GA 31216 PH: 478-788-1286 Email: [email protected]

GSH EDUCATION COORDINATOR and MICROTIME ASSOCIATE EDITOR Carl Sagasser Albany, GA PH: 229-317-6974 FX: 229-317-6682 Email: [email protected]

GSH SECRETARY & WEB MANAGER Shirley A. Powell Macon, GA PH: 478-301-2374 FX: 478-301-5489 Email: [email protected]edu

GSH PR CHAIR Fran Adams Riverdale, GA PH: 770-994-1360

GSH MEMBERSHIP CHAIR Wanda Simons Grayson, GA Emory Eastside Medical PH: 770-736-2565 FAX: 770-736-2368 Email: [email protected] GSH ASSISTANT MEMBERSHIP CHAIR Terri Brown Northside Hospital-Atlanta [email protected] PH: 404-845-5423 FAX: 404-303-3706

Email: [email protected]

GSH SYMPOSIUM REGISTRAR Harriett Baker Griffin, GA 770-229-6909 [email protected]




JOIN US FOR OUR ANNUAL MEETING March 25 - 27, 2011 at Callaway Gardens in Pine Mountain, Georgia The room rates will be $99 to include a continental breakfast and admission to the garden and its amenities. The rooms have been renovated and are absolutely gorgeous. For those of you who came the last time, the meeting rooms will be in the same area, but the rooms will be at the top near the meeting rooms. I want to encourage you to attend and participate in a great meeting. GET YOUR RESERVATIONS EARLY! We are looking for a theme for our meeting at Callaway Gardens in Pine Mountain in March, 25-27th. We are having a contest for the best theme for the meeting, awarding $100.00 for best one. Mike Ayers and Chris Coley will be the judges.


Please fill out all blanks COMPLETELY - PLEASE PRINT and Mail to: Wanda Simons, GSH Membership Chair 459 Bishopton Street Grayson, GA 30017

Name ____________________________________________________________ Home Address _______________________________________________________________ -

Home City ___________________________________ ST ___________ Zip _______ Home Phone _________________________________________Fax____________________ Home Email _______________________________________________________________ Employer __________________________________________________________ Work Address -_______________________________________________________________ Work City _______________________State ______________________Zip ________ Work Phone ___________________________________Fax ___________________ Work Email ______________________________________________________________ NSH MEMBER ______YES _______NO GSH recommends that you consider joining NSH in order to be eligible to serve as a representative of GSH in the House of Delegates at the annual Symposium and actively participate in your society. *******Date: _____________________________________ ****** REQUIRED Membership is free to anyone working in histology in Georgia but you must fill out a form each year to be a member. If you do not live in but work in Georgia, you qualify. If you do not work in Georgia you may still be a non-voting member with other member privileges such as discount rates at meetings. MEMBERSHIP YEAR IS FROM JANUARY TO DECEMBER Interested in serving on a committee? _______ Do you prefer mail sent to: Home?__ Work?__ It is very important to notify us of changes in your information as soon as possible so we can keep you informed.

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Friday, September 24, 2010

11:00am - 6:00pm 4:00pm ­ 5:00pm 5:00pm ­ 6:30pm 5:00pm ­ 5:45pm 6:45pm - 8:00pm Registration Opens Committee Chairs Meeting President's Council Meeting NSH Committee Meetings · PR · Membership First Time Attendee Welcome Reception

Saturday, September 25, 2010

7:00am - 5:00pm Registration

7:00am - 8:00am 8:00am - 11:30am 11:30am - 1:00pm 12:00pm ­ 12:45pm

1:00pm - 2:30pm 3:00pm ­ 4:30pm 4:35pm - 5:30pm 4:35pm - 5:30pm

Continental Breakfast Available Workshops Lunch on Your Own NSH Committee Meetings · Budget & Finance · Education/CEU · Quality Control · VIR Workshops (90 minute sessions new this year!) Workshops (90 minute sessions new this year!) NSH Region Meetings (I-IX) International Attendee Meeting

Sunday, September 26, 2010

7:00am - 5:00pm 7:00am - 8:00am 8:00am - 9:30am 9:30am - 4:30pm 9:45am - 10:15am 10:00am - 11:30am 12:30pm - 4:00pm 7:00pm ­ 11:30pm Registration Continental Breakfast Available NSH Lecture Series Exhibits Open for Attendees New Member Orientation Poster Sessions Workshops NSH Annual Leadership, Education & Advocacy Awards Banquet Histotech of the Year & Decade to be Awarded!


Monday, September 27, 2010


- 5:00pm 7:00am - 8:00am 8:00am - 5:00pm 8:00am - 11:30am 8:00am ­ 5:00pm 9:00am - 3:30pm 11:30am - 1:30pm 12:00pm ­ 12:45pm

1:30pm - 5:00pm 5:00pm - 6:00pm

Registration Continental Breakfast Available Board of Directors Meeting Workshops Educator's Forum Exhibits Open for Attendees Lunch On Your Own NSH Committee Meetings · Hard Tissue · IHC · Health & Safety · Legislation Workshops Resource Group Meetings: · Instructors of Histotechnology · Journal Editorial Board · State Newsletter Editors · State Meeting Planners

Tuesday, September 28, 2010

7:00am - 5:00pm 7:00am - 8:00am 8:00am - 11:30am 8:00am ­ 4:30pm 9:00am - 1:00pm 11:30am - 1:00pm 1:00pm - 4:30pm 4:45pm - 6:00pm 6:00pm - 7:00pm Registration Continental Breakfast Available Workshops Management Forum Last Day of Exhibits Lunch on Your Own Workshops NSH Membership Meeting NSH Committee Meetings: · Bylaws · Nominations & Elections

Wednesday, September 29, 2010

7:00am - 2:30pm 7:00am - 8:00am 8:00am - 11:30am 11:30am - 1:00pm 1:00pm - 4:30pm 6:00pm 7:00pm Registration Continental Breakfast Available Workshops Lunch On Your Own Workshops Sign In for House of Delegates House of Delegates Convenes

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Georgia Rep... Georgia Rep... Sharon Wehman [email protected] Sharon Wehman 678-462-6349 [email protected]


Mail Preference : All NSH Correspondence will be sent to this address. In addition this address is published in our online membership directory. Home Work

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Georgia Rep..... Sandy Schmitz 404-697-5262


Carole Fields, HT (ASCP) 962 Hickory Leaf Ct. Marietta, GA 30065



19 pages

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