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2nd International Symposium on Bio-nanosystems

October 31 ­ November 2, 2008 Koshiba Hall, the University of Tokyo, Hongo, Tokyo, Japan

Program and Abstracts

The Symposium is hosted by

MEXT, Scientific Research on Priority Areas: Bio-nanosystems (http://nanobio.phys.s.u-tokyo.ac.jp/NanoSystem/)

Correspondence:

Hideo Higuchi, Ph.D. Department of Physics, Graduate School of Science, the University of Tokyo 7-3-1 Hongo Bunkyou-ku Tokyo, Japan 113-0033 Phone: +81-35841-4128 Fax: +81-35841-7646 e-mail: [email protected]

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October 31st (i ) Welcome Address

13:00-13:05

October 31st (Friday)

Hideo Higuchi (University of Tokyo)

Session I. Actomyosin nanosystem

(Session Chair: Motoshi Kaya) 13:05-13:35 Motoshi Kaya (University of Tokyo) S1-1 Introduction Power stroke distance and load-dependent step size of single skeletal myosins So Nishikawa (Osaka University) S1-2 Single molecule detection of two-legged nano-machines Ronald Rock (University of Chicago) S1-3 Navigating the Cytoskeleton with Myosins Issei Mabuchi (Gakusyuin University) S1-4 Structure and assembly of actin cytoskeletons in fission yeast cells Takeyuki Wakabayashi (Teikyo University) S1-5 Tropomyosin-binding motifs of actin Taro Uyeda (AIST) S1-6 A novel expression system for toxic mutant actins, and characterization of mutant actins defective in motility.

13:35-14:05 14:05-14:45 14:45-15:15 15:15-15:30 15:30-15:45

15:45-16:00 16:00-18:00

Coffee Break Poster Session 1

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November 1st. (Satday November 1st (Saturday) ) Session II. Structure and Mechanism of Dynein

(Session Chair: Kazuhiro Oiwa) 9:00-9:05 9:05-9:40 9:40-10:05 10:05-10:40 Kazuhiro Oiwa (NICT) Introduction Stan Burgess (University of Leeds) S2-1 Structure and mechanism of dynein studied by electron microscopy Keiko Hirose (AIST) S2-2 Structural changes in dynein molecules bound to microtubules Daniela Nicastro (Brandeis University) S2-3 Three-dimensional structure of cytoplasmic and axonemal dynein bound to microtubules Yusuke Kato (University of Leeds) S2-4 Structure of the microtubule-binding domain of a flagellar dynein

10:40-10:55 10:55-11:10 11:10-11:35 11:35-11:50

Coffee Break

Takahide Kon (University of Tokyo) S2-5 Structure and mechanochemical cycle of cytoplasmic dynein Shiori Toba (NICT) S2-6 Mechanical and enzymatic properties of the heterodimeric axonemal dynein-f Kenji Imamula (University of Tokyo) S2-7 The stalk coiled coil of axonemal dynein is responsible for microtubule rotation Yuki Hirota (Nagoya City University) S2-8 Polarization of ependymal cilia during mouse brain development

11:50-12:05

12:05-12:20 12:20-13:50

Lunch (Put up posters in Poster session 2)

Session III. Dynein in Axonemes and Cells

(Session Chair, Kazuhiro Oiwa) 13:50-14:20 S2-9 Takashi Ishikawa (ETH Zürich) Structural study of outer and inner dynein arms by electron cryo-tomography Shinji Kamimura (Chuo University) S2-10 Dynamic features of the axonemal structure of sea-urchin sperm flagella revealed by small-angle X-ray diffraction analysis Yoko Toyoshima (University of Tokyo) S2-11 Reguration of dynein motility by LIS1 and NDEL1 Erika Holzbaur (University of Pennsylvania) S2-12 Flexibility in Dynein Motor Function at Obstacles and Intersections

14:20-14:45

14:45-15:20 15:20-15:45 15:45-16:00 16:00-18:00 18:30-20:30

Coffee Break Poster Session 2 Conference Dinner

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November 2

November 2nd (Sunday)

Session IV. Kinesin-microtubule nanosystem

(Session Chair: Michio Tomishige) 9:00-9:30 Michio Tomishige (University of Tokyo) S3-1 Introduction Single molecule observation of conformational changes of kinesin Hernando Sosa (Albert Einstein College of Medicine) S3-2 The kinesin-1 walking mechanism as revealed by ensemble and single-molecule fluorescence polarization microscopy

9:30-10:05

10:05-10:25 10:25-11:00

Coffee Break

Thomas Surrey (EMBL) S3-3 Microtubule plus-end tracking by EB1 and CLIP-170: Mechanistic insight from an in vitro reconstitution Gohta Goshima (Nagoya University) S3-4 Microtubule generation within the mitotic spindle Ayumu Yamamoto (Shizuoka University) S3-5 Meiotic telomere clustering depends on mircrotubule motors in fission yeast. Naofumi Handa (University of Tokyo) S3-6 Genomic ID sequence recognition by a genome maintenance enzyme

11:00-11:30 11:30-11:45

11:45-12:00

12:00-13:20

Lunch

Session V. Bio-nanosystems from Molecules to Cells

(Session Chair: Hideo Higuchi) 13:20-14:00 Keiichi Namba (Osaka University) S3-7 Molecular Mechanisms of Self-Assembly and Its Regulation of the Bacterial Flagellum Chikako Shingyoji (University of Tokyo) S3-8 Roles of motor proteins in the regulation of melanophore responses in zebrafish Hideo Higuchi (University of Tokyo) S3-9 Myosin, kinesin and dynein in in vitro and cells

14:00-14:30

14:30-15:00

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November 2

16:00-18:00

P1-1

Poster Session

Poster Session 1 (October 31st)

Takeshi Nakagawa, Hiroto Tanaka, Kazuo Sasaki (Tohoku University) Why can the energy consumption of myosin be reduced when they work together? Atsuko H. Iwane, Takako Ichinose, Masatoshi Nishikawa, Tomotaka Komori (Osaka University) The effect of the Magnesium ion in the ATPase activity and motility of MyosinVa Masayuki Takahashi, Takashi Nakasawa, Masaaki Sato, Yuusuke Kawashima, Fumiko Matsuzawa, Sei-ichi Aikawa, Michio Yazawa (Hokkaido University) Charge clusters in the C-terminal rod region are essential for assembly of vertebrate nonmuscle myosin II Shigehiko Yumura, Atushi Yata, Akira Nagasaki, Yoshiaki Iwadate (Yamaguchi University) Dynamics of myosin II filaments and myosin II heavy chain kinase C in live cells under TIRF microscopy Kazuhiro Kawanoue, Shigeru Chaen, Shinsaku Maruta (Soka University) Interaction of ATP driven motor proteins with ATP analogue having syn conformation with respect to the adenine-ribose bond Hideki Shishido, Masafumi D. Yamada, Kazunori Kondo, Shinsaku Maruta (Soka University) Photocontrol of Calmodulin Function using Photochromic Compound Ryoki Ishikawa, Ayumi Takahashi, Akio Nakamura, Kazuhiro Kohama (Gunma University) In vitro sliding on myosin II of F-actin under the conditions fully saturated with variety of actin-binding proteins Masak Takaine, Osamu Numata, Kentaro Nakano (University of Tsukuba) The fission yeast IQGAP, Rng2 is a monomeric actin-bundling protein required for contractile ring formation Shin-ichiro Kojima, Yvonne Aratyn, Danijela Vignjevic, Thomas Schaus (Northwestern University) Molecular basis of actin-bundling during filopodia protrusion.

P1-2

P1-3

P1-4

P1-5

P1-6

P1-7

P1-8

P1-9

P1-10 Masahiko Harata , Naoki Aoyama, Asako Oka, Kumiko Kitayama, Susan Gasser, Kenji Shimada, Yukako Oma (Tohoku University) Actin-related proteins involved in genome and chromosome functions P1-11 Hiroaki Kubota, Sergey V. Mikhailenko, Shin'ichi Ishiwata (Waseda University ) D-loop of actin is important for the motility of myosins II and V

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P1-12 Eri Nishihara, Makoto Tominaga, Hiroyuki Nakagawa (Fukuoka University) Neurite elongation from cultured insect neural cell P1-13 Hideki Nakanishi, Seisuke Arai, Tunehito Higashi, Ikuo Wada (Fukushima Medical University) Aberrant diffusion of secretory proteins in the endomembranes P1-14 Keisuke Ueda, Chieko Kimura, Masao Miki, Toshiaki Arata (Osaka University) Distribution of distance between spin-labeled tropomyosin and actin in reconstituted skeletal muscle thin filament by SDSL-ESR study P1-15 Kensaku Mizuno, Tai Kiuchi, Souichi Kurita, Kazumasa Ohashi (Tohoku University) Role of cofilin in actin filament assembly and disassembly during lamellipodium formation and extension P1-16 Kentaro Nakano and Masak Takaine (University of Tsukuba) Functional analysis of a novel ADF/cofilin-super family protein involved in the organization of actin cytoskeleton in fission yeast P1-17 Sinji Yoshiyama, Ryoki Ishikawa, Akio Nakamura, Sugie Higashi-Fumime, Hideyo Takatsuki, Kevin Rice, Eric. R.Blough, Kazuhiro Kohama (Gunma University) Actin-bundles as a transporter of nano-sized cargoes. P1-18 Togo Shimozawa and Shin'ichi Ishiwata (University of Tokyo) Detection of the structural distortion in a single actin filament induced by tensile force using fluorescence microscopy P1-19 Hironori Ueno, Takuo Yasunaga, Chikako Shingyoji and Keiko Hirose (AIST) Structural analysis of outer arm dynein molecules bound to microtubules P1-20 Takuo Yasunaga, Hiroko Takazaki, Yoshinori Ohta (Kyushu Institute of Technology) Image analysis systems from electron micrographs of motor proteins and their complexes P1-21 Izumi Nakano and Chikako Shingyoji (University of Tokyo) Effects of ATP-S on the microtubule-binding and the motile activity of 21S dynein of sea urchin sperm flagella at high ATP P1-22 Yumi Watanabe and Chikako Shingyoji (University of Tokyo) Conditions for the induction of continuous beating by externally applied bending in demembranated motionless sea urchin sperm flagella at low ATP P1-23 Hitoshi Sakakibara, Shiori Toba, Hiroyuki Iwamoto, Kazuhiro Oiwa (NICT) Helical Rearrangement of the Axonemal Components in Chlamydomonas flagella coupled with Ca2+ concentrations revealed by X-ray Fiber Diffractions

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P1-24 Katsutoshi Mizuno, Yuji Shitaka, Hitoshi Sakakibara, Kazuhiro Oiwa, Kazuo Inaba (University of Tsukuba) Calaxin is a potential Ca2+-dependent modulator of outer arm dynein P1-25 Kazuo Inaba, Mamoru Nomura, Akiko Hozumi, Lihong Zhu, Mia Nakachi (University of Tsukuba) Molecular signaling for the activation of flagella motility in the ascidian Ciona

intestinalis

P1-26 Kogiku Shiba, Shoji A Baba, Manabu Yoshida (University of Tokyo) Mechanism of sperm chemotaxis in the ascidian: When do spermatozoa detect the chemoattractant? P1-27 Toshiki Yagi, Keigo Uematsu, Zhongmei Liu, Ritsu Kamiya (Kyoto University) Identification of three novel dyneins localized only to the proximal region of Chlamydomonas flagella P1-28 Kazuo Sasaki and Hidemi Akiyama (Tohoku University) A unified theoretical model for "bipedal" molecular motors

Poster Session 2 (November 1st)

16:00-18:00

P2-1 M. D. Yamada, T. Aihara, K. Sugata, P. G. Fajer, M. Miki, S. Maruta, Toshiaki Arata (Osaka University) Conformational dynamics of unique elements of kinesin and troponin observed by spin-labeling ESR spectroscopy Satoshi Yasuda, Masafumi D. Yamada, Shinsaku Maruta, Toshiaki Arata (Osaka University) Study of the dynamic structure of kinesin by site-directed spin labeling ESR (electron spin resonance) Tsukasa Makino, Michio Tomishige, Masahide Kikkawa (University of Tokyo) Unique conformation of kinesin's neck linker in the nucleotide-free state Xiao Ling, Teppei Mori, Michiko Nakajima, Michio Tomishige (University of Tokyo) Observation of ATP binding to the individual motor domains of dimeric kinesin Teppei Mori, Michiko Nakajima, Michio Tomishige (University of Tokyo) Processive motility of heterodimeric kinesin that has defect in neck linker docking Ken'ya Furuta and Yoko Y. Toyoshima (University of Tokyo) Processive movement by a defined number of nonprocessive motor proteins Junichiro Yajima, Kana Mizutani, Takayuki Nishizaka (Gakushuin University) A torque component present in mitotic kinesin Eg5 revealed by three-dimensional tracking

P2-2

P2-3 P2-4

P2-5 P2-6 P2-7

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P2-8

Toshiki Taba, Masaki Edamatsu, Shiori Toba, Keitaro Shibata, Yasuhiro Imafuku, Yoko Yano Toyoshima, Katsuhisa Tawada, Akira Yamada (Kyushu University) Torsional Flexibility of Kinesin: Direction and Speed of Microtubule Movements Driven by Kinesin Motors Arranged on Catchin Filaments Atsuko Uchida, Nael Alami, Yoshio Imura, Akiko Oyabu, Anthnony Brown (Mie University) Tight functional coordination between KIF5A and dynein motors in the bidirectional transport of neurofilaments

P2-9

P2-10 Hiroyasu Hatakeyama, Hideaki Fujita, Tomonobu Watanabe, Hideo Higuchi, Makoto Kanzaki (Tohoku University) Derangement of GLUT4 movement under insulin resistant state in 3T3-L1 adipocytes ­ Qdot­based single molecule analysis of GLUT4 behavior ­ P2-11 Yuko Kubo, Masafumi D. Yamada, Yasunobu Sugimoto, Katsuzo Wakabayashi, Shinsaku Maruta (Soka University) Analysis of Conformational Change of Novel Rice Kinesin K16 Using Small Angle X-ray Solution Scattering P2-12 Masafumi D. Yamada, E-Shin Chia, Hidekatsu Maeda, Shinsaku Maruta (Soka University) Photoregulation of Kinesin Activity Using an Caged compounds P2-13 Eiichi Kobayashi, Masafumi Yamada, Masato Ito, Shinsaku Maruta (Soka University) Photo-control of ATPase activity of the kinesin motor domains intermolecularly cross-linked by bifunctional photochromic compound P2-14 Taketoshi Kambara, Jungwoo Yoo, Kohsuke Gonda, and Hideo Higuchi (University of Tokyo) Intracellular imaging of targeted proteins labeled with Quantum Dots. P2-15 Shigeaki Saitoh, Yasuyo Kobayashi, Yuki Ogiyama and Kohta Takahashi (KAN Research Institute) Dual regulation of Mad2 localization on kinetochores by Bub1 and Dam1/DASH that ensure proper spindle interaction P2-16 Hirotada Akiyama, Tomohiko J. Itoh, Takahito Higashi, Ryong-Woon Shin, Keiko Hirose, Akihiro Harada, Chiyoko Uchida, Takafumi Uchida (Tohoku University) hGas7b that controls microtubule dynamics is degraded in Alzheimer's brain P2-17 Hiroshi Ueda and Kaori Mizota (Nagasaki University) Neurosteroids Regulate Microtuble Dynamics through MAP2-Tubulin Affinity P2-18 Makoto Okuno and Chinatsu Mukai (University of Tokyo) Poly amino acid reduced bend amplitude but not beat frequency Modulation of

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P2-19 Yuko Miyamoto, Daisuke Yamauchi, Ryota Kuno, Tomonori Nakai, Norihiro Futamura, Kenji Shinohara, Teruo Shimmen, Masaki Shimamura, Tomohiro Akashi, Tetsuya Horio, Yoshinobu Mineyuki (University Hyogo) Plant -tubulins have gradually lost the function that is essential for unikonts cells during their evolution P2-20 Masaki Mishima, Ryoko Maesaki, Miyuki Kasa, Takashi Watanabe, Masaki Fukata, Kozo Kaibuchi, Toshio Hakoshima (Tokyo Metropolitan University) Structural basis for the recognition of -tubulin by the plus-end tracking protein CLIP-170 P2-21 Eiro Muneyuki, Shoichi Toyabe, Takahiro Watanabe-Nakayama University) Behavior of F1-ATPase motor under external torque (Chuo

P2-22 Ayumi Koike-Takeshita, Masasuke Yoshida, Hideki Taguchi (University of Tokyo) Revisiting the GroEL-GroES reaction cycle via the symmetrical intermediate implied by novel aspects of the GroEL (D398A) mutant P2-23 Madoka Suzuki, Vadim Tseeb, Kotaro Oyama, Kaoru Iwai, Shin'ichi Ishiwata (Waseda University) Modulation of intracellular Ca2+ by heat pulse P2-24 Tomonori Nakai, Miyuki Takeuchi, Hitoshi Sakakibara, Kazuhiro Oiwa, Naoko Kajimura, Yoshinobu Mineyuki (University of Hyogo) Bacterial cellulase in the cellulose synthesizing nanomachine and twisting of cellulose fibrils

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