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NMC 50th Annual Meeting January 23-26, 2011 Arlington, Virginia

Titles of Posters to be Presented at the Technology Transfer Session

1. Management Practices Associated with Acquisition of New Staphylococcus aureus Intramammary Infections Simon Dufour 1,2, Ian R. Dohoo 1,3, and Daniel T. Scholl 1,2, 1Canadian Bovine Mastitis Research Network, 2 Veterinary Medicine, University of Montreal, 3Atlantic Veterinary College, University of Prince Edward Island, St.-Hyacinthe, Quebec, Canada 2. Estimation of the Diagnostic Accuracy of a Multiplix Real-Time PCR Assay and Bacteriological Culture for Four Bovine Intramammary Pathogens M.E. Paradis1,4, S.P. Oliver2,4, B. Gillespie2, S. Messier 1,4, J. Comeau 3,4, and D.T. Scholl1,4, 1University of Montreal, St-Hyacinthe, Québec, Canada, 2University of Tennessee, Knoxville, Tennessee, USA, 3University of Sherbrooke, Sherbrooke, Québec, Canada, 4Canadian Bovine Mastitis Research Network 3. Consequence for Dairy Herds in the United States of Imposing Different Standards for Somatic Cell Count H. Duane Norman, Janice Wright, and Robert Miller, Animal Improvement Programs Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland, USA 4. Predicting Liner Performance Sonia Gomez, Doug Reinemann, and Paul Thompson, University of Wisconsin-Madison, Madison, Wisconsin, USA 5. Cow and Quarter Characteristics Associated with Teat Dimensions Ingrid Zwertvaegher1, Sarne De Vliegher2, Sofie Piepers2, Jeroen Baert1, and Stephanie Van Weyenberg1, 1 Institute for Agricultural and Fisheries Research (ILVO), 2Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium 6. Bacteriological Cure of Clinical Mastitis Cases in Wisconsin Dairy Herds ­ Preliminary Results Leane Oliveira, Carol Hulland, and Pamela L. Ruegg, University of Wisconsin, Madison, Wisconsin, USA 7. Molecular Characterization of Isolates from Pre-partum Secretions, Teat Skin Swabs and Post-partum Milk Samples from Replacement Heifers on a Grazing Dairy Davin R. Ringen, Doug Suntrup, and John R. Middleton, University of Missouri, College of Veterinary Medicine, Columbia, Missouri, USA 8. Prevalence And Antimicrobial Susceptibility of Mastitis-causing Bacteria in Dairies of Costa Rica Laura Solano¹, Mariano Rojas³, Elías Barquero1,2, Jaime Murillo¹, ¹Faculty of Veterinary Medicine, National University of Costa Rica, Heredia, Costa Rica, ²Research Program in Tropical Diseases, Heredia, Costa Rica, ³Dos Pinos Dairy Corporation, Alajuela, Costa Rica 9. Bovine Mastitis Detection by Real-time Polymerase Chain Reaction and Standard Microbiology in Early Lactation Quarter Samples Reha O. Azizoglu1, Rudy Rodriguez1,2, Roberta Lyman1, Debbie Asion2, Kevin L. Anderson1, 1North Carolina State University, Raleigh, North Carolina, USA, 2Advanced Animal Diagnostics, Durham, North Carolina, USA 10. The Effects of Flunixin Meglumine Treatment on Activity Measures And Feed Intake During Experimentally Induced Escherichia coli Mastitis Emily E. Yeiser, Christina S. Petersson-Wolfe, and Ken E. Leslie, Virginia Tech University, Blacksburg, Virginia, USA 11. Quantification of Aflatoxin M1 in Dairy Farms Located in Buenos Aires Province (Preliminary Study) C.N. Acuña Pasqualini, G.F. Casasnovas, and A. Magariños, PURO (United Milk Producers Cooperative from Route Eight) Laboratory, San Antonio de Areco, Argentina

12. Fly Repellent Efficacy of Barrier Teat Dip Products with Housefly a Comparative Evaluation of Some Commercial Products Fahim U Ahmed, Camelia N. Traistaru, Thomas C Hemling, DeLaval Manufacturing, MQAH R&D Department, Kansas City, Missouri, USA 13. Does Choice of Mastitis Therapy Make Any Difference? James Allcock, Alex Corbishley, and Rob Smith, Clover Data Management Ltd., Whitchurch, Shropshire, United Kingdom 14. In Vitro Efficacy of Commercial Teat Dips Against Prototheca Gilbert Bardoneschi and Joseph Morelli, Ecolab Inc., Châlons en Champagne, France; and Redmond, Washington, USA 15. Udder Health and Antibiotic Use can be Optimized by the Combined Use of Herd Navigator and On-farm Culture Torben W. Bennedsgaard1, and Jens Yde Blom2, 1Aarhus University, 2Biosens, Foulum, Denmark 16. Determination of Total DNA and Enumeration of Viable S. aureus from Bovine Subclinical Mastitis Bruno G. Botaro, Cristina S. Cortinhas, Larissa V. de Março, Luis F. P. Silva, José G. M. Franchini, Nilson R. Benites, and Marcos V. Santos, University of São Paulo-Pirassununga, São Paulo, Brazil 17. Effect of Payment System on Quality of Milk in Brazilian Dairy Herds Bruno G. Botaro, Augusto H. Gameiro, and Marcos V. Santos, University of São Paulo-Pirassununga, São Paulo, Brazil 18. Use of a Selective Agar for Detection of Prototheca in Bulk Tank and Cow Milk Culture A.M. Britten, Jon Cerar, and Murali Gurajala, Udder Health Systems, Inc., Boise, Idaho, USA 19. Prevalence of Mycoplasma spp. in Bulk Tank Milk from Dairy Farms of the Biobio Region of Chile Karina Bustos and Marcos Munoz, Facultad de Ciencias Veterinarias, Universidad de Concepción, Chillán, Chile 20. Milk Discard for Spiramycin, Neomycin and Flumethasone in Cows Treated by Intramammary Way G.F. Casasnovas1, D. Chan2, R.E. Chertcoff1, and C.N. Acuña Pasqualini1, 1United Milk Producers Cooperative from Route Eight, San Antonio de Arec, Argentina 2University of Buenos Aires, Buenos Aires, Argentina 21. Spiramycin-Neomycin Effectiveness for Clinical Mastitis Treatment Produced by Staphylococcus aureus Sensitive and Resistant to Penicillin G.F. Casasnovas, R.E. Chertcoff, M.C. Chiapello, C.N. Acuña Pasqualini, PURO (United Milk Producers Cooperative from Route Eight), San Antonio de Areco, Argentina 22. Propionibacterium Granulosum and Lipopolysaccharide of Escherichia coli Effectiveness in Clinical Mastitis Caused by Coliform Bacteria Treatment G.F. Casasnovas, and C.N. Acuña Pasqualini, PURO (United Milk Producers Cooperative from Route Eight), San Antonio de Areco, Argentina 23. Round Shaped Liners vs. Triangular Shaped Liner and Their Effect on the Teat End Condition in a Dairy Herd Located in Southeastern Sicily Difalco1, M. Gambina1, and G. Licitra1,2, 1CoRFiLaC, Regione Siciliana, Ragusa, Italy, 2D.A.C.P.A., Università di Catania, Catania, Italy 24. A Practitioner's Perspective of PCR Testing and its Comparison to Conventional Bacteriology Peter W. Edmondson, Shepton Veterinary Group, Shepton Mallet, Somerset, England 25. Objective Assessment of Pain in Dairy Cattle with Clinical Mastitis Colleen E. Fitzpatrick1, Ken E. Leslie1, Todd F. Duffield1, David F. Kelton1, Trevor J. DeVries1, Christina S. Petersson-Wolfe2, 1University of Guelph, Guelph, Ontario, Canada, 2Virginia Polytechnic Institute and State University, Blacksburg Virginia, USA

26. Correction of Inadequate Settings of Milking Systems on Sicilian Dairy Farms M. Gambina1, A. Difalco1, G. Licitra1,2, 1CoRFiLaC, Regione Siciliana, Ragusa, Italy, 2D.A.C.P.A., Università di Catania, Catania, Italy 27. Detection and Prevention of Milking Routine Problems by Computerized Monitoring of Milking Irregularities R. Ginsberg1, D. Rak2, A. Arazi2, and E. Aizinbud2, 1National Service for Udder Health and Milk Quality ­ Israel Dairy Board, Yehud, Israel, 2S.A.E Afikim, Kibbutz Afikim, Israel 28. An Evaluation of Bacterial Count in a Liner Before and After Spraying with Peracetic Acid Solution Richard Hiley1, Richard May1, Colin Kingston1, Ian Ohnstad2, 1Ambic Equipment Ltd., Witney, Oxfordshire, England, 2The Dairy Group, Taunton, Somerset, England 29. Efficacy of Vaccination on Mastitis Epidemiology: Field Study Luis Miguel Jiménez1, Carlos Romero2, 1Servet Talavera SL, Talavera de La Reina, Toledo, Spain2, Finca La Nava, Almagro, Ciudad Real, Spain 30. The Effect of Over-Pressure on Teat End Health Aaron K. Kochman, Lauren AgriSystems, New Philadelphia, Ohio, USA 31. In Vitro Susceptibility Testing of Coagulase Negative Staphylococcus (CNS) Isolated from Subclinical an Clinical Mastitis in Dairy Cows in Brazil Helio Langoni, Felipe Guimarães, Diego Nóbrega, and Paulo Domingues, School of Veterinary Medicine and Animal Science, São Paulo State University, Botucatu, Sao Paulo, Brazil 32. In Vitro Susceptibility Testing of Ceftiofur Against Bovine Mastitis Pathogens Isolated as Part of an Ongoing Surveillance Program (2002-2009) Cindy J. Lindeman, Lacie Johansen, Ellen S. Portis, and Lisa M. Mullins, Pfizer Animal Health, Kalamazoo, Michigan, USA 33. Yeast-like Mastitis Pathogens ­ Efficacy of Teat Disinfectants Mario G. Lopez Benavides, Carolina Mateus, and Tom Hemling, DeLaval Manufacturing, Kansas City, Missouri, USA 34. Evaluation of Teat Condition Using Liquid or Powder Dips in Winter Mario G. Lopez Benavides1, Kia Knutson2, Leo Timms2, Mark Henderson1, and Tom Hemling1, 1DeLaval Manufacturing, Kansas City, Missouri, USA, 2Iowa State University, Ames, Iowa, USA 35. Coagulase-Negative Staphylococci Enterotoxins Isolated from Bovine with Subclinical Mastitis Simone Lucheis1,2, Hélio Langoni1, Maria Cunha1, and Diego Nóbrega1, 1São Paulo State University, São Paulo, Brazil, 2Paulista Agency of Agribusiness Technology ­ APTA, Bauru, São Paulo, Brazil 36. SOP Treatment of Separate Manure Solids Reduced Klebsiella Bacteria Counts H. Lynn Sharkey1, A. Zanierato2, P. Luparia2, M. Poggianella2, P. Moroni 1, Y. H. Schukken1, 1Quality Milk Production Services, Cornell University, Ithaca, New York, USA, 2S.O.P. Srl, Busto Arsizio, Varese, Italy 37. Evaluation of the Efficacy of a New Vaccine Against Bovine Mastitis Caused by CNS Field Trial Results Marta Noguera, Ricard March, Roger Guix, Antoni Prenafeta, Antoni Foix, HIPRA, Amer, Girona, Spain 38. Incidence Rate and Risk Factors of Subclinical and Clinical Mastitis in Dutch Dairy Heifers Richard Olde Riekerink1, Inge Santman-Berends1, Otlis Sampimon1, and Theo Lam1,2, 1GD Animal Health Service, Deventer, the Netherlands, 2Dutch Udder Health Centre UGCN, Deventer, the Netherlands 39. Monitoring Clinical Mastitis Using Dairy Comp 305 Jose C. F. Pantoja, David A. Rhoda, Nigel B. Cook, and Pamela L. Ruegg, University of Wisconsin, Madison, Wisconsin, USA 40. Characterization of Abnormal Milk by Proteomic Methods Lotte Bach Larsen, and Morten Dam Rasmussen, Aarhus University, Research Centre Foulum, Tjele, Denmark

41. On-farm Antimicrobial Use and Antimicrobial Resistance in Bovine Mastitis Pathogens: The Canadian Scenario V. Saini1, J T. McClure2, D. Léger3, and H. W. Barkema1, 1 University of Calgary, Calgary, Alberta, Canada, 2 Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Prince Edward Island, Canada, 3 Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Ontario, Canada 42. Persistence of Candida Rugosa Intramammary Infection in a Dairy Herd L. Scaccabarozzi1, C. Locatelli1, G. Pisoni1, G. Manarolla1, A. Casula1, V. Bronzo1, P. Moroni1,2, 1University of Milan, Milan, Italy, 2Quality Milk Production Services, Cornell University, Ithaca, New York, USA 43. The Effects of Lauric Acid on Mastitis Pathogens C.S. Schneider1, L.K. Fox2, and A.N. Nristov3, 1University of Idaho, Moscow, Idaho, USA, 2Washington State University, Pullman, Washington, USA, 3Pennsylvania State University, University Park, Pennsylvania, USA 44. Dynamics of Subclinical Mastitis in Holstein Cows from Brazilian Dairy Herds with Low and High Bulk Tank Somatic Cell Counts Guilherme N. Souza1, José R. F. Brito2, Abdon Geber de Melo3, Geraldo Max Linhares3, Leonardo Leite Cardozo4, Samuel Miguel Hylario5, and Cleocy Fam de Mendonça Júnior6, 1Embrapa Gado de Leite, Juiz de Fora, Minas Gerais, Brazil, 2Pólo de Excelência do Leite, Juiz de Fora, Minas Gerais, Brazil, 3Centro Universitário Nilton Lins, Manaus, Amazonas, Brazil, 4UDESC, Lages, Santa Catarina, Brazil, 5Embrapa Gado de Leite/CNPq, Juiz de Fora, Minas Gerais, Brazil, 6Associação dos Criadores de Gado Holandês do Estado de Minas Gerais, Juiz de Fora, Minas Gerais, Brazil 45. Standardized Method for the Testing of Liner Mouthpiece Vacuum Under Field Conditions Brandon Treichler, and Paul Rapnicki, University of Minnesota College of Veterinary Medicine, St. Paul, Minnesota, USA 46. Biomolecular Monitoring Of Milk Quality: Standardization of Multiplex PCR for the Detection of Staphylococcus aureus, Streptococcus agalactiae and Escherichia coli in Bovine Milk Samples Obtained from Bulk Tanks Marcella Troncarelli, Virginia Richini-Pereira, and Helio Langoni, Universidade Estadual Paulista, Botucatu, Sao Paulo, Brazil 47. Combination Effects of Teat Spraying and Dry Cow Therapy Sally-Anne Turner, John Williamson, Kate Wynn, and Jane Lacy-Hulbert, DairyNZ, Hamilton, New Zealand 48. The Annual Monitoring of the SCC on a Commercial Italian Farm Treated with SOP C COW Andrea Zanierato1, Mauro Casalone2, Paola Luparia1, Valerio Bronzo3, and Paolo Moroni3,4, 1SOP s.r.l., Busto Arsizio, Varese, Italy, 2Free-lance veterinary, Torino, Italy, 3Università degli Studi di Milano, Veterinaria, Italy, 4 Quality Milk Production Services, Cornell University, Ithaca New York, USA 49. Pilot Study to Assess the Incidence of Staphylococcus aureus Infections in Newly Calved Dairy Heifers Using PCR on DHI Samples Ann Godkin1 and 2Richard Cantin, 1Ontario Ministry of Agriculture, Food and Rural Affairs, Elora, Ontario, Canada, 2CanWest DHI, Guelph, Ontario, Canada

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