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P-001: DYNAMICS OF REGULATORY ASPECT OF AYURVEDIC MEDICINE IN INDIA P-002: PIPERINE: A POTENT BIOAVAILABILITY AND BIOEFFICACY ENHANCER P-003: EFFECT OF SHODHANA AN ANCIENT PURIFICATION METHOD ON SEMECARPUS ANACARDIUM LINN. P-004: CHEMICAL AND MOLECULAR FINGERPRINT PROFILE OF OROXYLUM INDICUM L. VENT BY HPTLC & FTIR TECHNIQUES P-005: HERBAL INDIA: LEADING THE WORLD WITH STRONG ROOT OF TRADITIONAL MEDICINE P-006: COMPARATIVE IN VITRO ANTIOXIDANT ACTIVITY OF FLOWERS OF PLUMERIA RUBRA L. F. RUBRA AND PLUMERIA RUBRA F. LUTEA P-007: EVALUATION OF HEPATOPROTECTIVE ACTIVITY OF LUFFA ACUTANGULA VAR AMARA FRUIT (CURCUBITACEAE) P-008: EVALUATION OF ANTI INFLAMMATORY AND ANTHELMINTIC ACTIVITY OF SEEDS OF IPOMOEA HEDERACEA JACQ. (CONVOLVULACEAE) P-009: DEVELOPMENT AND CLINICAL EVALUATION OF HERBAL FORMULATIONS FOR LEUCODERMA P-010: PHYTOCHEMICAL INVESTIGATION & PHARMACOLOGICAL EVALUATION OF TRIANTHEMA PORTULACASTRUM LINN. (WEED) FOR ANTIASTHMATIC ACTIVITY P-011: DEVELOPMENT & EVALUATION OF POLYHERBAL FORMULATIONS FOR OSTEOARTHRITIS P-012: PRELIMINARY PHYTOCHEMICAL INVESTIGATION AND IMMUNOMUDULATORY ACTIVITY OF ETHANOLIC EXTRACT OF FICUS BENGALENSIS STEM BARK P-013: EVALUATION OF OINTMENT OF AZADIRACHTA INDICA LEAVES AND CURCUMA LONGA ROOTS (RHIZOMES) AS TOPICAL WOUND HEALING MEDICAMENT
P-014: MODELS FOR PRECLINICAL SCREENING OF HERBAL DRUGS AFFECTING SEXUALITY IN RODENTS: A REVIEW P-015: EVALUATION OF SAFETY AND TOXICITY OF HERBAL DRUGS P-016: EVALUATION OF DIURETIC POTENCY OF ROOT EXTRACT OF HEMIDESMUS INDICUS P-017: ANTI-ULCER ACTIVITY OF ANNONA RETICULATA SEED MUCILAGE P-018: STANDARDIZATION AND EVALUATION OF THE POLYHERBAL COUGH SYRUP P-019: LIQUORICE AUGMENTS PROTECTIVE ACTION OF VASAKA AGAINST CIGARETTE SMOKE INDUCED PULMONARY INFLAMMATION P-020: PHARMACOGNOSTICAL STANDARDIZATION OF VOLATILE OIL AND DETERMINATION OF ORTHOSIPHON THYMIFLORUS P-021: ISOLATION AND DETERMINATION OF TRITERPENOID FROM ROOTS OF HYPTIS SUAVEOLENS P-022: IN-VITRO ANTI-ARTHRITIC ACTIVITY OF HOLOPTELIA INTEGRIFOLIA LEAVES P-023: STANDARDIZATION OF PIPERINE CONTAINING HERBAL FORMULATIONS BY MODERN ANALYTICAL TECHNIQUES P-024: PERCEPTION OF MALAYSIAN MEDICAL STUDENTS ABOUT COMPLEMENTARY AND ALTERNATIVE MEDICINES P-025: PHARMACOGNOSTICAL AND PRELIMINARY PHYTOCHEMICAL PROFILING OF COLEUS AROMATICUS BENTH. P-026: PRELIMINARY PHARMACEUTICAL CHARACTERIZATION OF CASSIA AURICULATA FLOWER AS POTENTIAL PHARMACEUTICAL DYES: ACID-BASE INDICATOR P-027: IN-VITRO ANTI-OXIDANT ACTIVITY OF GMELINA ARBOREA FRUITS PERICARP EXTRACTS
P-028: SYNERGISTIC ANTI-OXIDANT AND PRO-OXIDANT ACTIVITIES OF SOME INDIGENOUS HERBAL FORMULATIONS P-029: LC-MS N-ALKYLAMIDE PROFILING OF AN ETHANOLIC ANACYCLUS PYRETHRUM ROOT EXTRACT P-030: ANTIDIABETIC ACTIVITY OF ANNONA CHERIMOLA P-031: STUDY OF A MIRACLE ALOE AN IMPORTANT MEDICINAL PLANT FOR ANTIDIABETIC ACTIVITY AND HISTOPATHOLOGY IN STREPTOZOTOCIN INDUCED DIABETES P-032: ANTICARIOGENIC ACTIVITY OF CALOTROPIS SP. PLANTS LATEX AND STRUCTURE ELUCIDATION OF BIOACTIVE COMPOUND P-033: ANTILITHIC ACTIVITY OF BOERHAAVIA DIFFUSA LINN, COMMIPHORA WIGHTII AND ROTULA AQUATICA LOUR ON URINARY TYPE STRUVITE CRYSTALS P-034: ANTI-ARTHRITIC ACTIVITY OF ALCOHOLIC AND WATER EXTRACT OF AN AYURVEDIC FORMULATION PANCASAMA CHURNA BY FREUND'S ADJUVANT INDUCED ARTHRITIS RATS MODEL P-035: EVALUATION OF ANXIOLYTIC ACTIVITY OF VARIOUS EXTRACTS OF AERIAL PART OF CLEOME GYNANDRA P-036: EVALUATION OF DIURETIC ACTIVITY OF AQUEOUS AND ETHANOLIC EXTRACTS OF COMMELINA DIFFUSA BURM F. IN RATS P-037: IN-VIVO AND IN-VITRO IMMUNOMODULATORY ACTIVITY OF METHANOLIC EXTRACTS OF SEEDS AND BARK OF PONGAMIA GLABRA VENT. P-038: ESTIMATION OF ANDROGRAPHOLIDE IN HEPATOPROTECTIVE FORMULATIONS USING HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHIC TECHNIQUE P-039: EVALUATION OF ANALGESIC, ANTIINFLAMMATORY AND ANTI-ARTHRITIC ACTIVITIES OF AQUEOUS EXTRACT OF AERIAL PART OF ERYTHRINA INDICA P-040: ANXIOLYTIC EFFECT OF LAGENARIA SICERARIA FRUITS ON THE ELEVATED PLUS MAZE MODEL OF ANXIETY IN RATS P-041: ANTI-INFLAMMATORY PROPERTIES OF THE LEAVES OF FICUS AMPLISSIMA SMITH. P-042: PROTECTIVE EFFECT OF AERVA LANATA (L) ON DOXORUBICIN INDUCED CARDIAC APOPTOSIS IN WISTAR RATS P-043: PHYTOCHEMICAL SCREENING, ANTIOXIDANT ACTIVITY AND HPTLC ANALYSIS OF TYLOPHORA PAUCIFLORA WIGHT & ARN AN IN VITRO STUDY P-044: EVALUATION OF IN VITRO PHYTOCHEMICAL SCREENING, CYTOTOXIC ACTIVITY AND FTIR ANALYSIS OF ALPINIA PURPURATA (VIEILL) K. SCHUM P-045: FREE RADICAL SCAVENGING ACTIVITIES AND HPTLC DETERMINATION OF CAYRATIA TRIFOLIA
P-046: CHEMICAL COMPOSITION AND ANTIOXIDANT ACTIVITY OF ESSENTIAL OIL FROM CURCUMA AMADA ROXB. P-047: EFFECT OF AQUEOUS EXTRACT OF HYBANTHUS ENNEASPERMUS IN GENTAMICIN INDUCED RENAL DAMAGE P-048: IMPROVED ABSORPTION AND BIOACTIVITY OF PLUMBAGIN FROM HYDROPHILIC LIPID BASED EXTRACT OF PLUMBAGO ZEYLANICA P-049: EVALUATION OF A NOVEL HERBAL OIL EXTRACT FORMULATION P-050: PHYTOCHEMICAL INVESTIGATIONS AND PHARMACOLOGICAL EVALUATION OF ZANTHOXYLUM RHETSA ROXB. (BARK) FOR ANTI-DIABETIC ACTIVITY P-051: EVALUATION AND FORMULATION OF NATURAL HAIR COLORANTS P-052: GLOBAL REGULATORY POSITION OF HERBAL MEDICINE: A REVIEW P-053: A STUDY OF EFFECT OF EXTRACTS OF CUSCUTA REFLEXA ROXB. ON CYCLOPHOSPHAMIDE INDUCED ALOPECIA P-054: POTENTIAL ROLE OF STRYCHNOUS POTATORUM EXTRACT ON DIABETES INDUCED RATS AND ITS HISTOPATHOLOGICAL ANALYSIS P-055: PHARMACOGNOSTIC EVALUATION AND ESTIMATION OF GALLIC ACID AND QUERCETIN IN CAREYA ARBOREA ROXB. LEAF BY HPTLC METHOD P-056: EVALUATION OF GENETIC DIVERSITY BY RAPD FINGERPRINTING IN THE GAMMA IRRADIATED MUTANT PLANTS OF JATROPHA CURCAS L P-057: ANTI ARTHRITIC ACTIVITY OF ALCOHOLIC AND WATER EXTRACTS OF CAESALPINIA DECAPETALA STEM FREUND'S ADJUVANT INDUCED ARTHRITIS RATS P-058: EVALUATION OF LIPID METABOLISM MODULATORY EFFECT OF A POLYHERBAL FORMULATION (ATH10) IN HYPERLIPIDAEMIC ANIMAL MODELS P-059: COMPARATIVE DIURETIC ACTIVITY OF PERICARP & SEED EXTRACTS OF TRAPA NATANS LINN P-060: ANTIOXIDANT ACTIVITY OF ISOLATED COMPOUNDS FROM ARTABOTRYS HEXAPETALUS LEAVES BY DIFFERENT METHODS P-061: PHYTOCHEMICAL AND PHARMACOLOGICAL EVALUATION OF CARTHAMUS TINCTORIUS LEAVES EXTRACT P-062: ADAPTOGENIC ACTIVITY OF POLYGONATUM VERTICILLATUM (L.) ALL. RHIZOME EXTRACTS IN RAT P-063: CHRONIC TOXICITY STUDIES OF PLUMERIA ACUMINATA AND GALEGA PURPUREA IN EXPERIMENTAL ANIMALS P-064: ISOLATION AND ESTIMATION OF PTERYGOSPERMIN IN MORINGA SPECIES AND MARKETED PREPARATION SEPTILIN BY HPTLC METHOD
P-065: PRELIMINARY PHYTOCHEMICAL SCREENING AND INVITRO ANTHELMINTIC ACTIVITY OF ARGEMONE MEXICANA ROOT P-066: FORMULATION OF AN EDIBLE, BIODEGRADABLE, ENVIRONMENTAL FRIENDLY, SAFE AND EFFECTIVE ANTI-ULCER CHEWING GUM P-067: APOPTOSIS STUDIES ON FLAVANOIDS ISOLATED FROM MEDICINAL PLANTS AGAINST HUMAN CANCER CELL LINE HepG2 P-068: HEPATOPROTECTIVE ACTIVITY OF ALCOHOLIC EXTRACT OF COLDENIA PROCUMBENS ON DGALACTOSAMINE INDUCED RATS P-069: NEPHROPROTECTIVE EFFECT OF TRIBULUS TERRESTRIS ON GENTAMICIN INDUCED KIDNEY DAMAGE P-070: ETHNOMEDICINAL PLANT KNOWLEDGE AND PRACTICE OF THE JASHPUR DISTRICTS OF CHHATTISGARH, INDIA P-072: IN VITRO ANTICOAGULANT ACTIVITY OF MANGROVE SPECIES AVICENNIA MARINA P-073: PHYTOCHEMISTRY AND COLUMN CHROMATOGRAPHY OF PHYSALIS MINIMA LEAVES P-074: PHARMACOGNOSTIC AND PRELIMINARY PHYTOCHEMICAL STUDY OF COMMELINA BENGHALENSIS STEM P-075: COMPARATIVE EVALUATION OF VARIOUS FRACTIONS ISOLATED FROM FENUGREEK FOR INFLAMMATORY DISORDERS P-076: QUALITY CONTROL PARAMETER AND LAXATIVE ACTIVITY OF SWADIST VIRECHAN CHURNA- A POLYHERBAL FORMULATION P-077: DEVEPLOPMENT OF QUALITY ASSESSMENT PARAMETERS FOR ANDROGRAPHIS PANICULATA P-078: SCREENING FOR ANTIMICROBIAL ACTIVITY AND PHYTOCHEMICAL CONSTITUENTS OF IXORA ALBA LINN. P-079: DEVEPLOPMENT OF QUALITY ASSESSMENT PARAMETERS FOR GYMNEMA SYLVESTRE P-080: SCREENING OF SELECTED HERBAL PLANTS FOR ANTI ACNE PROPERTIES P-081: ANTICANCER ACTIVITY OF PENTATROPHIS MICROPHYLLA P-082: STANDARDIZATION & ANTI ULCER ACTIVITY OF PEPGARD SYRUP-POLYHERB FORMULATION P-083: ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY OF MYRICA NAGI BARK P-084: ANTIULCER POTENTIAL OF STEM BARK OF BAUHINIA PURPUREA LINN. P-085: STANDARDIZATION OF NAVAYASA LAUHA CHURNA P-086: SCREENING OF ANTIDIABETIC ACTIVITY OF DESMOTRICHUM FIMBRIATUM BLUME P-087: ANTIASTHMATIC ACTIVITY OF BARK OF MYRICA NAGI THUNB.
P-088: PHYTOCHEMICAL INVESTIGATION AND IN VITRO BIOLOGICAL SCREENING OF MALLOTUS PHILIPPINENSIS P-089: EFFECT OF SHODHAN PROCESS ON STRYCHNINE CONTENT OF NUX VOMICA SEED P-090: EVALUATION OF PHYTOCHEMICAL AND PHYSICOCHEMICAL PARAMETERS OF ABHA CAPSULE P-091: PHYTOCHEMICAL INVESTIGATION & ANTIMICROBIAL ACTIVITY OF AJUGA BRACTEOSA WALL P-092: ESTIMATION OF TOTAL TANNIN CONTENT FROM RAW MATERIAL AND FORMULATIONS P-093: ANALGESIC ACTIVITY OF ESSENTIAL OIL EXTRACTED FROM COLEUS AROMATICUS LINN. LEAVES P-094: EVALUATION AND STANDARDIZATION OF VARIOUS MARKETED FORMULATION OF RAUWOLFIA P-095: HPTLC FRINGERPRINTING & ANTIHYPERLIPIDEMIC ACTIVITY OF GUAZUMA ULMIFOLIA LAM. LEAVES P-096: PHARMACOGNOSTICAL, PRELIMINARY PHYTOCHEMICAL AND ANTHELMINTIC STUDIES ON QUISQUALIS INDICA LEAF P-097: EVALUATION OF ANTIFUNGAL ACTIVITY OF OCIMUM SANCTUM LEAVES AGAINST HUMAN PATHOGENIC DERMATOPHYT ES P-098: STANDARDIZATION OF ARTHRUMPLUS CAPSULE - A POLYHERBAL FORMULATION P-099: THERAPEUTIC PROSPECTIVE OF APHANAMIXIS POLYSTACHYA AS AN ANTI-HIV AND ANTIOXIDANT P-100: IN VITRO ANTIOXIDANT ACTIVITY OF METHANOL EXTRACT OF CANTHIUM PARVIFLORUM P-101: GLORIOSA SUPERBA- A CRITICALLY ENDANGERED PLANT OF JHARKHAND AND WEST BENGAL P-102: HYPOLIPIDEMIC ACTIVITY OF DHATRINISHA CHURNA IN HIGH FAT DIET INDUCED HYPERLIPIDEMIC RATS P-103: IN VITRO ANTIOXIDANT STUDIES AND FTIR ANALYSIS ON ETHANOLIC EXTRACT OF ZALEYA DECANDRA P-104: LIPID LOWERING ACTIVITY OF FERONIA LIMONIA LINN. LEAF IN TRITON WR-1339 INDUCED HYPERLIPIDEMIA IN RATS P-105: ANTIOBESITY AND ANTIHYPERLIPIDEMIC EFFECTS OF HIBISCUS ROSA SINENSIS LINN. LEAF EXTRACT ON HIGH FAT DIET INDUCED OBESE RATS P-106: PHYTOPHARMACOLOGICAL EVALUATION OF SOLANUM SISYMBRIIFOLIUM LAM. FRUITS WITH SPECIAL REFERENCE TO ITS ANTI-DEPRESSION AND ANXIOLYTIC ACTIVITY P-107: BIOACTIVE COMPOUNDS FROM HIMALAYAN MEDICINAL PLANTS P-108: STANDARDIZATION OF PANCHKOLA CHURNA AND SIMULTANEOUS ESTIMATION OF PIPERIN & PLUMBAGIN BY HPLC METHOD
P-109: EFFECT OF FAGONIA CRETICA LINN IN THE IN VITRO INHIBITION OF CHOLESTEROL CRYSTALS P-110: IMMUNOMODULATORY ACTIVITY OF BARK OF THESPESIA POPULNEA LINN. P-111: PHYTOCHEMICAL AND PHARMACOLOGICAL EVALUATION OF FICUS LYRATA LEAVES EXTRACT P-112: EVALUATION OF DIURETIC ACTIVITY OF ETHANOLIC EXTRACT OF ACORUS CALAMUS LINN. RHIZOME P-113: QUANTITATIVE ESTIMATION OF PHYTOCHEMICALS AND ANTIOXIDANT ACTIVITY OF ROOTS OF JASMINUM SAMBAC P-114: PRELIMINARY PHYTOCHEMICAL SCREENING AND ANTIOXIDANT POTENTIAL OF ROOTS OF ALBIZZIA LEBBECK (L.) BENTH. P-115: PREPARATION AND EVALUATION OF POLYHERBAL FORMULATION FOR THE MANAGEMENT OF OSTEOPOROSIS P-116: SCREENING OF THE INDIGENOUS MEDICINAL PLANTS FOR CHOLESTEROL ESTERASE INHIBITION ACTIVITY P-117: EVALUATION OF ACETYLCHOLINE ESTERASE IINHIBITORY ACTIVITY OF SELECTED MEDICINAL PLANTS P-118: BIOACTIVITY GUIDED ISOLATION OF ANTIDERMATOPHYTIC PRINCIPLES FROM COCONUT SHELL P-119: BIO-ACTIVE GUIDED FRACTIONATION OF OCIMUM BASILICUM FOR ITS NEPHROPROTECTIVE ACTIVITY P-120: STANDARDIZATION OF SAMSHAMNI VATI P-121: PREPARATION AND EVALUATION OF POLYHERBAL SYRUP FOR TREATMENT OF PYREXIA P-122: TO PREPARE AND EVALUATE POLYHERBAL FORMULATION FOR THE MANAGEMENT OF NEPHROPROTECTIVE ACTIVITY P-123: SCREENING OF IN VITRO ANTIMALARIAL ACTIVITY OF SOME SELECTED PLANT EXTRACTS AND THEIR COMBINATIONS P-124: SCREENING OF IN VITRO ANTIMALARIAL ACTIVITY OF SOME SELECTED PLANT EXTRACTS AND THEIR COMBINATIONS P-125: ANTIMICROBIAL AND HEMOLYSIS STUDY OF PURE AND ZINC DOPED NANO-HYDROXYAPATITE P-126: EFFECT OF RASAYAN CHURNA IN EXPERIMENTAL CIRRHOTIC ACSITES- A POSSIBLE HOPE FOR HEPATORENAL FAILURE P-127: PHARMACOGNOSTIC AND PHYSICO-CHEMICAL PARAMETERS OF CORDIA DICHOTOMA G. FORST STEM BARK P-128: EVALUATION OF CLASSICAL AYURVEDIC FORMULATION VARUNADI KWATH FOR ITS ANTILITHIATIC ACTIVITY
P-129: ANTIOXIDANT AND ANTICANCER PRPOERTY OF SEMECARPUS ANACARDIUM EXTRACT IN B16F10 MELANOMA CELL LINES P-130: WONDERFUL ANTICANCER DRUG TAXOL P-131: CYTOGENETIC EVALUATION FOR CHEMOPROTECTIVE POTENTIAL OF SOME TRADITIONALLY USED HERB AS TONIC P-132: A RAY OF HOPE FOR HIV PATIENTS CALANOLIDES P-133: CHROMATOGRAPHIC FINGER PRINT STUDIES OF STEROIDS IN ZANTHOXYLUM RHETSA ROXB (DC) BY HPTLC TECHNIQUE P-134: IN-VITRO EVALUATION OF RADICAL SCAVENGING ACTIVITY OF DIFFERENT SUCCESSIVE LEAVES EXTRACTS OF PLUMERIA OBTUSA P-135: PRODUCT DEVELOPMENT AND STANDARDISATION OF AN ANTIPYRETIC POLYHERBAL FORMULATION P-136: ANTIFERTILITY ACTIVITY OF ETHANOL EXTRACT AND ITS FRACTIONS OF MENTHA ARVENSIS LEAVES IN FEMALE RATS P-137: AN ETHNOMEDICINAL STUDY ON TRADITIONAL REMEDY USED IN ARTHRITIS AND RELATED DISORDER BY MISHING TRIBE IN ASSAM P-138: PHYOCHEMICAL SCREENING, ANTIOXIDANT ACTIVITY AND ANTIMICROBIAL ACTIVITY OF SPATHODEA CAMPUNALATA (STEM +BARK) EXTRACTS P-139: TOXICITY STUDY AND ANTINOCICEPTIVE ACTIVITY OF GMELINA ARBOREA EXTRACT AND ITS FRACTIONS P-140: ANTIMICROBIAL EVALUATION OF SOME PHYTOCHEMICALS OF WITHANIA SOMNIFERA P-141: CHEMOTAXONOMIC STUDY ON FEW SEED TAXA OF FAMILY SAPOTACEAE USED AS FOLKLORE MEDICINES P-142: EXTRACTION OF ANTIMICROBIAL METABOLITES FROM HALO-TOLERANT ALKALIPHILIC ACTINOMYCETES ISOLATED FROM THE GREAT RANN OF KUTCH P-143: A COMPLETE REVIEW ON AN IMPORTANT MEDICINAL PLANT: ENICOSTEMMA LITTORALE BLUME P-144: IMMUNOMODULATORY ACTIYITY OF BARK OF THESPESIA POPULNEA LINN P-145: ISOLATION AND CHARACTERIZATION OF "THE KING OF BITTERS" BY DIFFERENT ANALYTICAL TECHNIQUES P-146: MANAGEMENT OF COMMON RESPIRATORY PROBLEM IN CHILDREN A SIDDHA THERAPEUTIC APPROACH P-147: SIDDHA APPROACH IN PAEDIATRIC CARE P-148: PHYSICO-CHEMICAL ANALYSIS OF "THIRITHODA MATHIRAI" A TRADITIONAL SIDDHA MEDICINE IN THE TREATMENT OF VARIOUS DISEASES
P-149: GASTROPROTECTIVE AND PROBABLE MECHANISTIC ACTIVITY OF THE ETHANOLIC ROOT EXTRACT OF POTENTILLA POLYPHYLLA WALL P-150: STANDARDIZATION OF POLYHERBAL FORMULATION FOR PSORIASIS P-151: A PHARMACOGNOSTICAL STUDY ON CAPPARIS DECIDUA EDGEW. STEM P-152: A CLINICAL STUDY TO COMBACT THE SIDE EFFECT OF RADIATION WITH ASWGANDHA IN ADVANCED CASE OF HEAD & NECK CANCER AND CERVICAL P-153: ANTI-DIABETIC ACTIVITY OF AMOMUM SUBULATUM ROXB. FRUIT CONSTITUENTS P-154: TRADITIONAL PHYTO-THERAPIES IN LIVESTOCK ALMENTS CARE AMONG THE NATIVE INHABITANTS OF IMAMPUR GHAT AREAS IN AHMEDNAGAR DISTRICT, (M.S.) INDIA P-155: PHARMACOGNOSTICAL EVALUATION OF BLEPHARISPERMUM SUBSESSILE DC LEAF P-156: MANGIFERIN HERBOSOMES AS A POTENT HEPATOPROTECTIVE AGENT P-157: ECOGEOGRAPHICAL DIVERSITY OF BACOPA MONNIERI IN RELATION TO QUANTITATIVE VARIATIONS OF BACOSIDE A P-158: CALLUS CULTURE STUDIES ON JASMINUM MALABARICUM - AN ENDEMIC MEDICINAL PLANT P-159: STUDY OF ENDOPHYTIC FUNGI ISOLATED FROM LEAVES OF HYDNOCARPUS PENTANDRA P-160: WOUND HEALING POTENTIAL OF OCIMUM BASILICUM (LINN.) IN INCISION WOUND MODEL IN RATS P-161: CHEMICAL COMPOSITION OF THE ESSENTIAL OILS OF TWO SALVIA SPECIES FROM IRAN FLORA P-162: COMPARATIVE PRELIMINARY ANTIINFLAMMATORY ACTIVITY OF SOLANUM SISYMBRIIFOLIUM (LAM.) LEAF, STEM AND ROOT P-163: BIO-ACTIVITY DIRECTED ISOLATION OF INDOLE ALKALOID FROM ALSTONIA SCHOLARIS STEM BARK FOR BROAD SPECTRUM ANTI-BACTERIAL AGENT P-164: IDENTIFICATION OF TERMINALIA ARJUNA ADULTERANTS USING CLASSICAL TAXONOMY AND CHEMOTAXONOMY P-165: DEVELOPMENT OF QUALITY CONTROL PARAMETERS FOR AN AYURVEDIC FORMULATIONAVIPATTIKAR CHURNA P-166: ANTIBACTERIAL ACTIVITY OF STEM BARK AND STEM WOOD OF NOTHAPODYTES NIMMONIANA P-167: ANTI-ALZHEIMER ACTIVITY OF CENTELLA ASIATICA - MODERN SCIENTIFIC EVALUATION P-168: ESTIMATION OF STIGMASTEROL AND SALICYLIC ACID IN LEPTADENIA RETICULATA (JIVANTI) AND ITS SUBSTITUTES P-169: PHARMACOGNOSTICAL STUDIES OF HYMENODICTYON ORIXENCE (ROXB.) STEM BARK
P-170: EVALUATION OF CERTAIN HERBAL REMEDIES SELECTED FROM LIBYAN MARKET P-171: IDENTIFICATION OF SOME PHYLLANTHUS SPECIES USING CLASSICAL TAXONOMY AND CHEMOTAXONOMY P-172: PHYTOCHEMICAL AND PHARMACOLOGICAL EVALUATION OF SOLANUM MELONGENA LEAVES P-173: PHARMACOGNOSTICAL STUDIES AND HPTLC FINGERPRINT OF AN AYURVEDIC POLYHERBAL FORMULATION-SITOPALADI CHURNA P-174: EFFECT OF ETHANOLIC EXTRACT OF PAEDERIA FOETIDA LINN. LEAVES ON SEXUAL BEHAVIOR AND SPERMATOGENESIS OF MALE RATS P-175: CAMPTOTHECIN PRODUCTION BY NOTHAPODYTES NIMMONIANA IN-VITRO CELL CULTURES P-176: EVALUATION FOR THE ANTI UROLITHIATIC ACTIVITY OF LUANAEA PROCUMBENS AGAINST ETHYLENE GLYCOL INDUCED RENAL CALCULI IN RATS P-177: IN VITRO & IN VIVO FREE RADICAL SCAVENGING ACTIVITY OF AMARANTHUS VIRIDIS L. P-178: ANTIDIABETIC AND IN VIVO ANTIOXIDANT ACTIVITY OF METHANOL EXTRACTS OF AGNOSMA CYMOSA ROXB. IN STREPTOZOTOCIN INDUCED DIABETIC MODEL P-179: DEVELOPMENT OF FINGER PRINTING METHODS OF AN AYURVEDIC FORMULATION PANCASAMA CHURNA: A SPECTROPHOTOMETRIC APPROACH P-180: ISOLATION OF ALOCASIA INDICA LINN. STARCH AND ITS PERFORMANCE AS A DISINTEGRATING AGENT P-181: FRUIT PEELS AS A NOVEL SOURCE OF ANTIOXIDANT AND ANTIMICROBIAL AGENTS P-182: RADICAL SCAVENGING AND ANTIOXIDANT ACTIVITIES OF PSIDIUM GUAJAVA L. (MYRTACEAE) LEAVES: A MEDICINAL PLANT FROM INDIA P-183: EFFECT OF CISSUS QUADRANGULARIS L. ON BIOCHEMICAL CHANGES INDUCED BY CARBON TETRACHLORIDE (CCL4) IN WISTAR ALBINO RATS P-184: NUTRACEUTICAL BOTTLE GOURD AS A POTENTIAL SOURCE OF NATURAL ANTIMICROBIAL AGENT P-185: STABILIZATION OF HEPATITIS -B SURFACE ANTIGEN (HBSAG) AT WATER IN OIL INTERFACE FOR ENCAPSULATION IN POLYMERIC MICROSPHERES P-186: AMELIORATIVE EFFECT OF BUTEA FRONDOSA (LEAVES) EXTRACT AGAINST SCOPOLAMINE INDUCED AMNESIA P-187: EVALUATION FOR ANTI-INFLAMMATORY ACTIVITY OF ETHYL ACETATE FRACTION OF TEPHROSIA PURPUREA P-188: COMPARATIVE STUDY OF HERBAL TERPENES AS PERMEATION ENHANCERS IN TRANSDERMAL DRUG DELIVERY OF GRANISETRON HCL.
P-189: PHARMACOGNOSTIC EVALUATION OF BERGIA SUFFRUTICOSA (DELILE) FENZL WHOLE PLANT, AN HITHERTO UNEXPLORED PLANT P-190: PHARMACOGNOSY CONCEPTS IN AYURVEDA AND AYURVEDIC DRUG STANDARDIZATION P-191: HEPATOPROTECTIVE ACTIVITY OF SEEDS OF CASSIA TORA LINN. P-192: LIMITATIONS OF CONVENTIONAL DRUG DISCOVERY METHODS AND OPEN OPTIONS TO EXPLORE AYURVEDA P-193: ANTHELMINTIC ACTIVITIES OF THE CRUDE EXTRACTS OF ALLIUM CEPA BULBS AND ELLETATRIA CARDOMOMUM SEEDS P-194: RATIONALITY FOR SELECTION OF HERBS FOR AYURVEDIC FORMULATION USED IN GI DISORDERS P-195: PHARMACOGNOSTICAL EVALUATION OF HOLOPTELEA INTEGRIFOLIA (ROXB.) PLANCH LEAF P-196: ANTI - LIPID PEROXIDATIVE PROPERTY OF SOME PHYTOCONSTITUENTS P-197: COMPARITIVE IN VITRO ANTI CANCER ACTIVITY OF COCCULUS HIRSUTUS AND CISSAMEPLOS PAREIRA P-198: NUTRACEUTICALS - AN AYURVEDIC APPROACH P-199: CNS ACTIVITIES OF DIFFERENT PLANT SOURCES OF SHANKHPUSHPI: A COMPARATIVE STUDY P-200: WOUND HEALING ACTIVITY OF LEAVES EXTRACT OF CASSIA OCCIDENTALIS LINN. P-201: ANTICONVULSANT ACTIVITY OF LEAVES OF CAPPARIS DIVARICATA LAM. P-202: PHARMACOGNOSTICAL EVALUATION OF CORDIA MACLEODII HOOK LEAF THROUGH DNA FINGER PRINTING P-203: EVALUATION OF HEPATOPROTECTIVE ACTIVITY OF ROYLEA ELEGANS (AERIAL PARTS) P-204: HPTLC AND HPLC METHODS FOR DETERMINATION OF LUTEOLIN IN HERBAL EXTRACT AND FORMULATION CONTAINING ECLIPTA ALBA LINN. P-205: OPTIMIZATION AND STANDARDIZATION OF ANTIDIABETIC POLYHERBAL FORMULATION P-206: EVALUATION OF ANTINFLAMMATORY ACTIVITY OF CASSIA OBTUSIFOLIA (LEAVES) P-207: EVALUATION OF COMPARATIVE PHARMACOGNOSTICAL AND PHOTOCHEMICAL STUDY OF ROOTS OF WILD AND CULTIVATED MUCUNA PRURIENS HOOK P-208: PHARMACOGNOSTICAL STUDIES OF RUELLIA PROSTRATA POIR AND ITS BIOLOGICAL EVALUATION FOR ANTI-INFLAMMATORY POTENTIAL IN WISTAR ALBINO RATS P-209: AYURVEDIC PERSPECTIVES ON HERBAL GALACTOGOGUES P-210: A SCIENTIFIC PHARMACOGNOSTICAL APPROACH ON VARIOUS PARTS OF JYOTISMATI (CELASTRUS PANICULATUS WILLD.)- A POLYGAMODIOECIOUS PLANT
P-211: REVIEW ON AMALAKI (EMBLICA OFFICINALIS GAERTN. ) AND ITS EFFECTIVE FORMULATIONS IN AYURVEDA: A POTENT CELL PROTECTOR P-212: DEVELOPED NATURAL SKIN CARE PRODUCTS FROM TRADITIONAL AND HERBAL MEDICINES P-213: MEDICINAL USES OF ARECA CATECHU P-214: ANTIOXIDANT PROPERTIES OF SOME THERAPEUTICALLY ACTIVE TRADITIONAL MEDICINAL PLANTS - AN OVERVIEW P-215: PERCEPTION OF ANTIOXIDANTS TO SCAVENGE FREE RADICALS IN HUMAN BODY P-216: DNA FOOTPRINTING IN MEDICINAL PLANTS P-217: EXPRESSION AND FUNCTIONALITY CHLOROPLAST GENOME TO DERIVE VACCINES AND BIOPHARMACEUTICALS - A REVIEW P-218: LICHENS AS TREASURE CHESTS OF NATURAL ANTIMICROBICS P-219: AN OVERVIEW: MOLECULAR FARMING FOR NEW DRUGS AND VACCINES P-220: TURMERIC IS THE ANTI-AGING, ANTI-OXIDANT, ANTI-INFLAMMATORY SUPER SPICE P-221: ANTI-ANXIETY POTENTIAL OF PASSIFLORA FOETIDA (AERIAL PARTS) P-222: ETHNO-HYPOGLYCEMIC GRASSES AND MANAGEMENT OF DM II P-223: DOCUMENTATION OF ETHNO-HEPATIC PLANTS FROM SOUTH-EAST RAJASTHAN P-224: IS AYURVEDA A COMPLIMENTARY SYSTEM OF MEDICINE? P-225: ANTI-INFLAMMATORY ACTIVITY OF AMAVATAVIDHAVANSA RASA P-226: COMPARATIVE STUDY OF ELEMENTS PROFILE OF JUGLANS REGIA (WALNUT), PRUNUS DULCIS (ALMOND) AND ARACHIS HYPOGAEA (PEANUT) OF INDIA P-227: A NEW EMERGING TECHNIQUE FOR SEPARATION OF ACTIVE INGREDIENT FROM MEDICINAL PLANT A WAY FOR DRUG DEVELOPMENT: SEPBOX P-228: SPIRULLINA SUPPLEMENT AS MEDICINE P-229: SIGNATURE ON THE SAFETY OF HERBAL DRUGS FOR HYPERTENSION P-230: PHYTOCHEMICAL AND PHARMACOLOGICAL POTENTIAL OF STRYCHNOS POTATORUM: A REVIEW P-231: HEPATOPROTECTIVE ACTIVITY EVALUATION OF HEDYCHIUM SPICATUM IN CARBON TETRACHLORIDE AND PARACETAMOL INTOXICATED RAT MODELS P-232: A REVIEW OF FICUS RELIGIOSA LINN. BASED ON ITS PHARMACOGNOSY, PHYTOCHEMISTRY AND PHARMACOLOGICAL USES P-233: POLYPHENOL RICH BOERHAAVIA DIFFUSA L. EXTRACT AMELIORATES OXIDATIVE STRESS IN ANGIOTENSIN II INDUCED CARDIAC HYPERTROPHY IN H9c2 CELLS.
P-234: PHARMACOGNOSTICAL PROFILE OF DESMODIUM GANGETICUM DC. AND DESMODIUM TRIFLORUM DC. P-235: A REVIEW OF FICUS GLOMERATA LINN. BASED ON ITS PHARMACOGNOSY, PHYTOCHEMISTRY AND PHARMACOLOGICAL USES P-236: ENHANCEMENT OF CURCUMIN ACTVITY BY COMPLEXATION WITH FERROUS ION P-237: EFFECT OF ABSCISSIC ACID, A PHYTO-HORMONE, IN FRUCTOSE-INDUCED INSULIN RESISTANT RATS P-238: ASSESSMENT OF SOIL PROPERTIES & ELEMENTAL COMPOSITION OF SOIL OF AN ORCHARD LOCATED AT BICHOLIN-GOA P-239: SIDDHA MEDICINAL PLANTS IN DERMATOLOGICAL DISORDERS P-240: PHARMACOGNOSTICAL AND BIOLOGICAL EVALUATION OF ARGYREIA NERVOSA LEAVES FOR ANTIULCER ACTIVITY IN WISTAR RATS P-241: MICROPROPAGATION OF SAFED MUSLI (CHLOROPHYTUM BORIVILIANUM) & STUDIES ON GENETIC INTEGRITY OF MICROPROPAGATED PLANTS THROUGH RAPD P-242: ETHNOPHARMACOLOGICAL STUDIES ON MYRICA ESCULENTA BUCH-HAM. STEM BARK A NOVEL ANTIPYRETIC HERBAL DRUG USED BY THE NAGA TRIBES OF MANIPUR P-243: MEDICINAL PLANTS AND CANCER CHEMOPREVENTION P-244: ARABINOGALACTAN: A THERAPEUTIC POTENTIAL BIO MOLECULE P-245: HEPATOPROTECTIVE ACTIVITY OF ETHANOL EXTRACT OF BERBERIS CORIACEAE IN CCl4 TREATED MALE RATS P-246: POLYHERBAL FORMULATION PROTECTIVE AGAINST ETHYLENE GLYCOL INDUCED UROLITHIASIS IN RATS P-247: GLOBAL REGULATIONS AND QUALITY STANDARDS FOR VALIDATION OF ANALYTICAL METHODS P-248: EVALUATION OF ANTIUROLITHIATIC ACTIVITY OF AQUEOUS EXTRACT OF ABUTILON INDICUM (L.) SWEET LEAVES P-249: MEDICINAL PLANTS AS A SOURCE OF ANTICANCER DRUGS: A REVIEW P-250: ASPARAGUS RACEMOSUS-A PROSPECTIVE MEDICINAL PLANT AS ADDITIONAL SOURCE OF INCOME FOR MARGINAL FARMERS P-251: ANALGESIC ACTIVITY OF ESSENTIAL OIL EXTRACTED FROM COLEUS AROMATICUS LINN. LEAVES P-252: IN SILICO ANALYSIS TO EVALUATE THE DRUGGABILITY OF PIPER NIGRUM P-253: EXENATIDE PROTECTS ISOPROTERENOL INDUCED LEFT VENTRICULAR HYPERTROPHY IN RATS
P-254: ROLE OF PUERARIA TUBEROSA DC EXTRACT ON ANDROGENESIS AND SEXUAL BEHAVIOR VIA FSH LH CASCADE P-255: A PILOT STUDY TO COMBAT THE SIDE EFFECT OF RADIATION WITH ASHWAGANDHA IN ADVANCED CASE OF HEAD & NECK CANCER & CERVICAL CARCINOMA P-256: EVALUATION OF ANTI-INFLAMMATORY ACTIVITY OF SOME LIBYAN MEDICINAL PLANTS IN EXPERIMENTAL ANIMALS P-257: PREVENTIONAL APPROACH FOR CANCER BY USE OF NUTRACEUTICALS P-258: ANTICARIOGENIC AND HEMOLYTIC ACTIVITY OF SELECTED SEED PROTEIN EXTRACTS AND THEIR POTENTIAL USE IN TOOTH INFECTION P-259: AQUASOMES: A NOVEL DRUG CARRIER P-260: PHYTOCHEMICAL STUDIES AND IMMUNOMODULATORY ACTIVITY OF ROOT BARK STREBLUS ASPER LOUR. P-261: ETHOSOMES: A NOVEL DRUG DELIVERY SYSTEM P-262: THERAPEUTIC POTENTIAL OF PHYTOALEXINS P-263: PHARMACOLOGICAL EVALUATION OF GMELINA ARBOREA FOR ITS ANTI CONVULSANT ACTIVITY P-264: A REVIEW OF PHYLLANTHUS AMARUS BASED ON ITS PHARMACOGNOSY, PHYTOCHEMISTRY AND PHARMACOLOGICAL USES P-265: WOUND HEALING POTENTIAL OF "TYPHIN" P-266: ANALYTICAL METHOD DEVELOPMENT AND EVALUATION OF EFFECT OF GERMINATION ON DIOSGENIN CONTENT IN FENUGREEK SEED BY HPTLC P-267: PHARMACOGNOSTIC EVALUATION OF PLUCHEA ARGUTA BOISS P-268: OBESITY: A CONCERN ON HUMAN HEALTH AND ITS MECHANISM P-269: SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF OMEPRAZOLE AND ONDANSETRON FROM COMBINED TABLET DOSAGE FORM P-270: SOLASODINE: A NATURAL STEROID: A REVIEW P-271: EFFECTIVE ROLE OF ACHYRANTHUS ASPERA SEED L. EXTRACT AND ITS ISOLATED CRUDE SAPONINS ON LIVER AND AORTA OF CHOLESTEROL TREATED RATS P-272: EVALUATION OF HEPATOPROTECTIVE ACTIVITY OF LEAF OF LAUNAEA PROCUMBENS (ROXB.) P-273: VITAMIN A PROTECTIVE AGAINST ISOPROTERENOL INDUCED MYOCARDIAL INFARCTION P-274: PHYTOCHEMICAL INVESTIGATION AND ANALGESIC PROPERTIES OF HIBISCUS CANNABINUS P-275: EVALUATION OF ANTI-INFLAMMATORY AND ANALGESIC PROPERTIES OF EVOLVULUS ALSINOIDES P-276: DEVELOPMENT OF ECONOMICAL NUTRACEUTICAL PRODUCTS FOR RURAL POPULATION
P-277: ANTIHYPERTENSIVE ACTIVITY OF ACORUS CALAMUS ON EXPERIMENTALLY INDUCED HYPERTENSION P-278: EVALUATION OF ANTIBACTERIAL ACTIVITY OF PANCHAVALKAL EXTRACT AND GEL FORMULATION AGAINST MULTIPLE DRUG RESISTANT STRAINS OF S. AUREUS P-279: NUTRACEUTICALS CURRENT MARKET STATUS & REGULATORY GUIDELINES P-280: ESTIMATION OF ASIATIC ACID IN GUM RESIN OF SHOREA ROBUSTA AND ITS VARIOUS MARKET FORMULATIONS P-281: TOTAL PHENOLIC ESTIMATION AND EVALUATION OF ANTIOXIDANT ACTIVITY OF DILLENIA INDICA P-282: PHYTOSOMES: A NOVEL DRUG DELIVERY SYSTEM FOR HERBAL MEDICINES P-283: ASSESSMENT OF SOIL PROPERTIES & ELEMENTAL COMPOSITION OF SOIL OF AN ORCHARD LOCATED AT BICHOLIN-GOA P-284: COMPARATIVE STUDY OF ELEMENTAL PROFILE OF JUGLANS REGIA (WALNUT), PRUNUS DULCIS (ALMOND) AND ARACHIS HYPOGAEA (PEANUT) OF INDIA P-286: FORMULATION OF SOLID ORAL DOSAGE FORM OF ETHANOLIC EXTRACT OF BOERHAAVIA DIFFUSA L. ROOTS P-287: ASSESSMENT OF ADAPTOGENIC POTENTIAL OF METHANOLIC EXTRACT OF RASAYANA DRUG JIVANTI P-288: DEVELOPMENT OF COMPUTER AIDED TECHNIQUE FOR THE IDENTIFICATION OF SELECTED HERBAL DRUGS P-289: PHARMACOGNOSTICAL EVALUTION OF SEEDS OF LEPIDIUM SATIVUM LINN. (BRASSICACEAE) P-290: HERB-DRUG INTERACTION P-291: PRECLINICAL EVALUATION FOR DETERMINATION OF ANTIVENOM POTENTIAL OF CYNODON DACTYLON P-293: COMPARISION OF PHENOLIC CONTENTS AND INVITRO ANTI-OXIDANT ACTIVITY OF VARIOUS EXTRACT OF ROOT FROM ANISOMELES INDICA LINN P-294: ISOLATION AND STANDARDIZATION OF UREASE FROM JACK BEAN P-295: ANTI BACTERIAL ACTIVITY OF SEAWEED EXTRACTS AGAINST PATHOGENIC BACTERIA
P-001: DYNAMICS OF REGULATORY ASPECT OF AYURVEDIC MEDICINE IN INDIA Chirag M. Modi, et al M.V.SC., Department of Pharmacology and Toxicology Herbal drug products constitute a major share of all the officially recognized system of health in India Viz. Ayurveda, Yoga, Naturopathy, Homeopathy, Siddha, Unani. The regulation of herbal drugs in crucial with respect to its safety, efficacy, quality and commercialization in domestic market as well as international market. Since long the appropriate laws/regulations have been in place but due to change in global trends of regulation of traditional medicine in India need to be focused for intestine priority for drug developers, their users, produces and marketing community. The optimum knowledge of regulatory affairs of herbal medicine is need of time for student, teachers and research. The current law recognizes the extrapolation of the safe use of Ayurvedic products and hence does not ask for new safety and risk assessments. In response changes in technology in productive and formulation of herbal drugs and evolution of newer combination of herbal drugs, many changes have been made by government in provision for rules related to proprietary Ayurvedic medicine. The inclusions of 3 more new categories of Ayurvedic medicine namely Balva / Poshak / Dravya / Soundary Prasadhan and Satva Ghane have been defined. The use of water as well as hydrophilicity solvent system (extract) has been permitted with some additional safety data apart from documented evidences. Adoption of Good manufacturing practice (GMP), modern packing technology, labeling of product with shelf life were made mandatory. A number of others changes new measures and provisions are already notified or under discuss in detail the genesis dynamics of regulation of Ayurvedic medicine, its current status and its impact on trade and business at domestic as well as international level. Keywords: Regulatory Aspect, Ayurvedic medicine, Herbal Drugs P-002: PIPERINE: A POTENT BIOAVAILABILITY AND BIOEFFICACY ENHANCER Ghanshyam B. Dudhatra, et al M.V.SC., Department of Pharmacology and Toxicology Piperine, an alkaloid having purity of 98% is obtained from Piper longum L. and Piper nigrum L. Indian Scientists at the Regional Research laboratory, Jammu (RRL, now known as Indian Institute of Integrative Medicine) discovered and validated Piperine as the world's first herbal bioenhancer in 1979. It acts as an antimicrobial bioenhancer as well as a nutritional bioenhancer. It also exhibits a variety of biological activities which includes antipyretic activity, antimetastatic activity, antithyroid activity, antitumor activity, antioxidative activity, antiapoptotic activity and antidepressant activity. It enhances bioavailability and bioefficacy of many drugs like phenytoin, propranolol, theophylline, rifampicin, oxytetracycline, ciprofloxacin, pefloxacin, nevirapine, amoxycillin, cefotaxime and, carbamazepine, and nutrients like coenzyme Q10, amino acids, vitamins, minerals and certain herbal compounds. It is the potent non competitive inhibitor of drug metabolizing enzymes like arylhydrocarbon hydroxylase (AHH), uridine diphosphate glucuronyl transferase (UDP-GT), ethylmorphine-N-demethylase, 7-ethoxycoumarin-O-deethylase, UDP-glucose dehydrogenase (UDPGDH), P-glycoprotein and cytochrome P450 subfamily CYP3A4. By inhibiting drug metabolism, piperine may increase the bioavailability of various compounds and alter the effectiveness of some medications. It is the most studied bioenhancer compound till date. Piperine being potent bioavailability and bioefficacy enhancer has potential future in human and veterinary therapeutics where bioavailability of some promising drug is lower and this is a major reason for therapeutic failure. The present paper describes the detailed account of bioavailability and bioefficacy enhancing potential of piperine, its future application in pharmaceutical industries and its outcomes to improve future of existing pharmacotherapeutics. Keywords: Bioavailability, Bioefficacy, Piperine, Bioenhancer P-003: EFFECT OF SHODHANA AN ANCIENT PURIFICATION METHOD ON SEMECARPUS ANACARDIUM LINN. Umang Gajjar1, K. M. Khambholja2, R. K. Patel2, S. S. Pancholi1 1Babaria Institute of Pharmacy, Vadodara, Gujarat; 2SKPCPER, Kherva, Gujarat Semecarpus anacardium if used without purification causes toxic skin inflammation problem because it contain toxic phenolic oil. During this Shodhana Process An ancient purification method toxic phenolic oil was removed, have marked effect on the concentration of the phytoconstituent & Antioxidant activity of Semecarpus anacardium. Total phenolic content 0.08660 %w/w), while 0.4163 %w/w to 8.940 decrease up to 70 % (from 28.960 there is a negligible effect on the concentration of total flavonoid (7.513 0.02623 0.004680 %w/w) and total carbohydrate (0.9077 0.006518 %w/w to 7.435 0.01828 % w/w) content. IC50%w/w to 0.8531 & EC50 value of extract of Semecarpus anacardium before and after purification are 171.73 & 314.3 while EC50 value are 280.28 mcg/ml & 304.46 mcg/ml, shows that antioxidant activity of Semecarpus anacardium is decreased but the safety profile of the drug is increased as the toxic phenolic oil was removed during Shodhana An Ancient purification method. Keywords: Semecarpus anacardium, Shodhana, toxicity P-004: CHEMICAL AND MOLECULAR FINGERPRINT PROFILE OF OROXYLUM INDICUM L. VENT BY HPTLC & FTIR TECHNIQUES Saraf Aparna A and K.Srilatha Srinivas The Institute of Science, Madam Cama road, Mumbai
Establishment of fingerprint profile of Oroxylum indicum L Vent using HPTLC & FTIR technique was carried out. Phytochemical screening and HPTLC studies were carried out by the methods of Harborne and Wagner. The solvent system employed for analysis of flavonoids was Chloroform: Methanol: Formic acid (8.8:0.7:0.5). Chrysin was used as marker flavonoid. Molecular fingerprint was obtained by FTIR technique. The current study was carried out to develop chemical and molecular fingerprints of the medicinally important plant Oroxylum indicum L Vent collected from different geographic regions of India. Such chemical and molecular fingerprint can be utilized for authentication and standardization of Oroxylum indicum L. Vent and also acts as a biochemical marker for this medicinally important plant which is utilized in the pharma industry. Keywords: HPTLC, FTIR, Chrysin, Oroxylum indicum L Vent P-005: HERBAL INDIA: LEADING THE WORLD WITH STRONG ROOT OF TRADITIONAL MEDICINE Pushpendra Kumar Patel, B.K. Dubey, Narendra Kumar Prajapati, Jyoti Sahu T.I.T. College of Pharmacy Bhopal (M.P.) India is a vast repository of medicinal plants that are used in traditional medical treatments. India gave `Ayurveda' the oldest golden book in herbal medicine system. The various indigenous systems such as Siddha, Ayurveda, and Unani use several plant species to treat different ailments. Medicinal plants play a vital role for the development of new drugs. Natural products from plant, animal and minerals have been the basis of the treatment of human disease but plants stands above all. Traditional knowledge can serve as powerful search engine, which will greatly facilitate intentional, focused and safe natural product drug discovery. These traditions have relatively organized database, and more exhaustive description of botanical material. People of small village are directly in touch with herbs and plant for treatment purpose, so we have to work together for better result. Although the herbal medicine doesn't have side effect but we should follow the strict quality control process. Globalization of herbal medicine system comes with the better advancement. All country must share their knowledge to make the world as herbal world. In present scenario the other medicinal system are in their declining phase due to their toxic effect, the herbal medicine system has a bright future to deals with the health, but still it has to face new challenges. It has to build its own place in peoples mind. Yet there is lot of scope for India for drug discovery and development for traditional medicine and to lead for the healthy world. Keywords: Ayurveda, traditional medicine, globalization, herbal world P-006: COMPARATIVE IN VITRO ANTIOXIDANT ACTIVITY OF FLOWERS OF PLUMERIA RUBRA L. F. RUBRA AND PLUMERIA RUBRA F. LUTEA Y Rajendra, P Gomathi and K Sirisha* Department of Pharm. chemistry, Vaagdevi College of Pharmacy, Hanamkonda- A.P. Background: The genus Plumeria (family: Apocynaceae) originated from Central America and consists of about eight species of which many are widely distributed in tropical countries. Plumeria rubra L., one of the species of the genus is an important medicinal plant, widely distributed throughout Southern India. In traditional medicinal system different parts of this plant have been mentioned to be useful in a wide variety of diseases. The plant material is widely used as a febrifuge, remedy for diarrhoea and cure for itch. The milky juice is employed for the treatment of inflammation and rheumatism. The leaves are reported to possess antiinflammatory, analgesic and antipyretic effects. Its branches are used as an abortive. The flowers have been reported to exhibit hypolipidemic effect in diabetes mellitus. Objective: To comparatively evaluate the antioxidant, free radical scavenging activities of the methanolic flower extracts of two cultivars of P. rubra (Plumeria rubra f. rubra and Plumeria rubra f. lutea). Materials and Methods: Shade dried flowers of both the cultivars of P.rubra were extracted with methanol and evaluated for antioxidant and free radical scavenging activities by DPPH, nitric oxide, H2O2 radical scavenging assays, reducing power assay, total phenol and flavonoid content assays. Results: Methanolic extracts of Plumeria rubra f. rubra and Plumeria rubra f. lutea (MEPR & MEPL) exhibited a strong reducing power and free radical scavenging ability. However, MEPR was found to be more active than MEPL in all the above methods except for their reducing ability and nitric oxide scavenging activity. Antioxidant activity of both the extracts was found to increase with an increase in concentration from 20-100g/ml. The results obtained in all the assays were highly significant and comparable to that of standard, ascorbic acid. Conclusion: The extracts have the antioxidant potential and can be considered as a source of natural antioxidants. P-007: EVALUATION OF HEPATOPROTECTIVE ACTIVITY OF LUFFA ACUTANGULA VAR AMARA FRUIT (CURCUBITACEAE) Diti I. Patel, Jagruti P. Modi, Vaishali N. Shah APMC College of Parmaceutical Education & Research, Himmatnagar, Gujarat Fruit of Luffa acutangula var amara belonging to family curcubitaceae commonly known as kadvighisodi reported in Ayurveda for different activities like jaundice, bronchitis, urinary discharge, piles. Systematic studies with respect to standardization of this drug are not reported in literature. The present study deals with the hepatoprotective activity of aqueous fruit extract (100, 200,300 mg/kg p.o) of luffa acutangula against CCl4 & paracetamol induced toxicity. Silymarin was used as reference drug. Various biochemical parameters like aspartate transaminase (AST), alanine transaminase (ALT), alanine phosphatase (ALP) and histopathological study was done. In order to elucidate the protection mechanism of drug extract against oxidative stress, lipid peroxide level (MDA) and antioxidant enzymes (SOD, CAT, and GSH) activities were determine simultaneously. The results showed hepatoprotective activity of aqueous extract was due to decreased levels of serum marker
enzymes viz., AST, ALT, ALP, bilirubin and increased total protein as compared to the toxicant group. Histopathological study also supports the hepatoprotective activity of fruit. Our result showed that this hepatoprotective effect of aqueous extract of fruit may be due to its antioxidant and free radical scavenging properties. Keywords: Hepatoprotective, Antioxidant, Luffa acutangula, Serum marker enzymes. P-008: EVALUATION OF ANTI INFLAMMATORY AND ANTHELMINTIC ACTIVITY OF SEEDS OF IPOMOEA HEDERACEA JACQ. (CONVOLVULACEAE) Disha I.Patel, Jyoti R.Joshi, Vaishali N. Shah APMC College of Pharmaceutical Education & Research, Himmatnagar, Gujarat In the present study seeds of Ipomoea hederacea commonly known as kaladana belonging to family Convolvulaceae have been extensively studied for its anti inflammatory & anthelmintic activity. This drug is described in Makhzan-d-Adwiya as a drastic purgative & attenuant relieving the system of Pitta and kafa and acting as an anthelmintic. Preliminary Phytochemical screening showed the presence of phytoconstituents like fatty acids, alkaloids, tannins, flavonoids and mucilage. Anthelmintic assay was performed by using various concentrations of different extracts (50,100,150 mg/ml) on the adult Indian earthworm Pheretima Posthuma. This bioassay involved determination of time of paralysis and time of death of the worms and compared with reference drug Albendazole. anti inflammatory activity was also estimated with aqueous extract (200 & 300 mg/kg) of seed by using rat paw edema model and percentage inhibition was calculated and compared with aspirin as a reference drug. Results showed that the aqueous extract exhibit highest anthelmintic activity compared to other extract in dose dependent manner. It also showed significant (p<0.01) anti-inflammatory activity in dose dependent manner. These activities may be due to the presences of phytoconstituents like polyphenols. Keywords: Ipomoea hederaceae, anthelmintic, anti-inflammatory P-009: DEVELOPMENT AND CLINICAL EVALUATION OF HERBAL FORMULATIONS FOR LEUCODERMA Parul P. Patel, Sanjogata M. Soni, Vaishali N. Shah APMC College of Pharmaceutical Education & Research, Himatnagar, Gujarat Leucoderma is a common dermatologic finding and one that has been observed since ancient times. It is characterized by white patches in the skin, caused by the loss of functioning melanocytes. Allopathic treatments for leucoderma are only suppressive therapy not curative. Ayurveda is proven to be very useful in treatment of leucoderma. So the present study showed the development of herbal formulations in form of Vati & Lepa. Vati consist of the Psoralea fruit powder, Gandhak, Red clay, Cow's ghee and Cow's urine. Lepa consist of Asafoetida and Cow's ghee. Developed formulation was standardized by phytochemical evaluation and clinical evaluation. The preliminary phytochemical screening was carried out by TLC and chemical test for the determination of various phytoconstituents. Clinical study of formulations was conducted on 16 patients having evidence of disease. All patients followed up for 3 months. The parameters like size of patch, formation of new patches, intensity of patches etc. were measured. Phytochemical evaluation showed the presence of furanocoumarins, fixed oils and fats in Vati and free ferulic acid in lepa. Results of clinical study showed that 81.75% patients got significant recovery rate with respect to repigmentation in leucoderma. It concludes that these formulations are safe to use and they produce the pigmentation in hypopigmented lesions of the skin. Keywords: Leucoderma, Psoralea, Asafoetida, Vati, Lepa. P-010: PHYTOCHEMICAL INVESTIGATION & PHARMACOLOGICAL EVALUATION OF TRIANTHEMA PORTULACASTRUM LINN. (WEED) FOR ANTIASTHMATIC ACTIVITY Shivanand V.H1 Dr Manjunath K.P2, Dr R.V Savadi2, A. V Bandarkar2 1N.E.T Pharmacy College, Raichur; 2K.L.E.S's college of pharmacy, vidyanagar, Hubli Trianthema portulacastrum Linn ( Fam: Aizoaceae.) Is a traditional medicinal plant with multiple utility that has been proved to have anti asthmatic, anti allergic activity Pharmacogonostic and pharmacological Investigation of the whole plant will give more information and this would be helpful in the isolation of biologically active compounds. Methanol was subjected for mast cell stabilization activity challenging rat mesenteric bits with mast cell degranulator compound 48/80 (4g/ml) under In vitro condition in comparison with standard disodium chromoglycate (40g/ml). Methanolic extract of weed has shown maximum activity in the concentration of (10, 20, 50 and100 g/ml) 34.94, 41.74, 53.92 and 64.04 % protection respectively. Standard has got 74.71 % protection. Considering the extractive value of methanol extract was taken for further study. Fractionation of Methanolic extract with ethyl acetate soluble further subjecting for column chromatography yields 48 major eluents. Fraction 11, 16 and 18 and Fraction 18-23 were pooled and subjected for spectral studies i.e. U.V, IR, 1H-NMR HPLC and MASS. Keywords: Trianthema portulacastrum Linn, Antiasthma, mast cell stabilization. P-011: DEVELOPMENT & EVALUATION OF POLYHERBAL FORMULATIONS FOR OSTEOARTHRITIS Bhumi M.Shah, Ekta K.Patel, Vaishali N.Shah APMC College of Pharmaceutical Education & Research, Himmatnagar, Gujarat Osteoarthritis is derived from the Greek word meaning inflammation of the bone joint. Osteoarthritis is one of the most common diseases throughout the world. It commonly affects the hips, feet, spine, large weight bearing
joint & knee. The conventional therapies are effective in alleviating symptoms but the efficacy is limited & not persistent. Many of the indigenous herbal preparations used by the tribes are little known to the public due to lack of scientific evaluation. The polyherbal formulations capsule & oil were developed by using the powders of Vitex negundo, Smilax china, Trachyspermum ammi, Tinosporea cordifolia, Boerhaavia diffusa, Curcuma longa & Zingiber officinalis. The preliminary phytochemical screening was performed. For Capsule parameters like wt.variation & disintegration time & for oil, acid value, iodine value etc. were performed. The quantification of total polyphenol & flavonoids were measured. Clinical study of formulations was conducted on 19 patients. All patients followed up for 3 months. Phytochemical screening shows Saponins, alkaloids, cardiac glycosides, bitter principle, anthrons & flavonoid present in this formulation. The clinical study showed that there was significant reduction in the parameters like severity of pain, joint swelling, muscle rubbing, etc. Results showed that the capsule provide effects may be due to the synergistic activity like anti-inflammatory, anti-arthritis, immunomodulatory effects of phytoconstituents like vitexin, curcumin, 6-gingerol etc. to reduce inflammation & pain among osteoarthritis patients. The oil acts as a synovial fluid & gives relief from pain. Thus, in this study there was an excellent relief from knee pain at the end of therapy & an overall improvement in quality of life was seen in patients after treatment. Keywords: Osteoarthritis, Polyherbal formulations, Clinical evaluation. P-012: PRELIMINARY PHYTOCHEMICAL INVESTIGATION AND IMMUNOMUDULATORY ACTIVITY OF ETHANOLIC EXTRACT OF FICUS BENGALENSIS STEM BARK Sameksha koul*, A. Pandurangan, R. L. Khosa School of Pharmacy, Bharat Institute of Technology, Partapur, Meerut Objective: The present study was designed to investigate the preliminary phytochemical and Immunomodulatrory activity of Ethanolic extract of Ficus bengalensis stem bark. Material and methods: The dried stem bark of Ficus bengalensis was pulverized, extracted with ethanol and was freeze dried. The ethanolic extract of Ficus bengalensis in two different doses of 200 mg/kg and 400 mg/kg of bodyweight was tested for its Immunomodulatory actions on Swiss albino rats weighing 150-200g. Different parameters studied were Delayed type hypersensitivity, TLC, DLC and RBC count. Result: The results obtained from the animals that received higher dose of ethanolic extract, revealed the fact there was a highly significant increase in the mean percentage of lymphocytes, 8.95 x 103 cells/ cumm ± 0.28 and significant increase in the mean percentage of neutrophils 50.33±0.88 when compared to control 4.78 x 103 cells/ cu mm ± 0.44 and 41.67±0.88 respectively. Also there was a significant increase in RBC count. Preliminary phytochemical investigations showed presence of flavanoids, steroids and tannins. Conclusion: Thus the study provides the immunomodulatory actions of the ethanolic extract of Ficus bengalensis stem bark. Keywords: Immunomodulation, Ficus bengalensis, DTH, TLC, DLC, RBC. P-013: EVALUATION OF OINTMENT OF AZADIRACHTA INDICA LEAVES AND CURCUMA LONGA ROOTS (RHIZOMES) AS TOPICAL WOUND HEALING MEDICAMENT Bharat Jhanwar, R.L. Soni, Renu Solanki 1Lachoo Memorial College of Sc.& Tech.,Jodhpur; 2College of Veterinary & Animal Sc., Rajasthan Agricultural Uni.(Raj) Fresh leaves of Azadirachta indica were washed with distilled water. Leaves were shade dried and powdered by grinding machine. Similarly, a fine powder of Curcuma longa rhizomes prepared. Leaves and rhizome powder each 3% w/w was mixed together with sterilized petroleum jelly to get desired ointment. Then was evaluated on six healthy male buffalo-calves for topical wound healing activity. Full thickness cutaneous excisional open wounds of size 2.5 x 2.5 cm were imposed on the thoraco- lumbar region of the dorsal median plane of the animal.. The wound on right region was treated with the ointment and left region served as control by using petroleum jelly. The gross evaluations were done by clinical observations like colour, granulation formation, epithelial growth formation, % rate of wound healing upto 20 days. Histopathological and Biochemical analysis viz. hydroxproline and hexosamine were also performed.Gross evaluation indicated surface of wound as moist reddish colour. Later thin scabs were formed on wound edges. Granulation tissues were apparent for 4 5 days. After 10 days wound cavity were filled by granulation tissues. Histopatholgical evaluation showed that test treatment ointment enhanced tissue repair. Biochemical test showed increased concentration level of hydroxproline and hexosamine as compared to control. The rate and quality of wound healing was more in Azadirachta indica - Curcuma longa ointment as compared to control. Keywords: Azadirachta indica, Curcuma longa, wound healing. P-014: MODELS FOR PRECLINICAL SCREENING OF HERBAL DRUGS AFFECTING SEXUALITY IN RODENTS: A REVIEW Virendra G. Kagathara, Kiran K. Shah, Indermeet Singh Anand, Neeraj S. Vyawahare, Tejal G. Soni Department of Pharmacology, Faculty of Pharmacy, Dharmsinh Desai University, Nadiad Studies of sexual behavior are particularly suitable for analyses of behavioral and neurobiological mechanisms of motivation. Epidemiological studies have shown that sexual desire disorders are common, particularly in women. Psychological treatments for such disorders are time consuming, and the outcome is uncertain. Therefore, it is very likely that many individual would prefer a reliable pharmacological and herbal treatment without side effects. The systemic development of such treatment requires undoubtedly a good preclinical model. On the other hand, the valid and reliable model of rodent sexuality would be of great utility to study the
behavioral and neurobiological basis of sexual motivation, due to similarities in basic neural and behavioral mechanisms controlling sexual desire in humans and rodents. In light of this, the present review focuses on the various preclinical screening models and methodologies used to evaluate and screen the herbal drugs and synthetic medicines likely to affect the various aspects of sexual behavior in rodents. Keywords: Behavioral, Epidemiological, neurobiological, preclinical model P-015: EVALUATION OF SAFETY AND TOXICITY OF HERBAL DRUGS Navneet Sharma, B.P. Nagori, Renu Solanki, Bharat Jhanwar Lachoo Memorial College of Science and Technology, Pharmacy wing, Jodhpur, Rajasthan Introduction: Herbal drugs are widely used for treatment and prevention of various diseases. Many people think being natural they are safe and free from undesirable side effects. However they may result in adverse effects. Therefore, this review focuses on the evaluation of safety and toxicity of herbal drugs. There are In vitro and in vivo toxicity studies which help in evaluation of herbal drugs. The test studies show that most of toxic effects are associated with hepatotoxicity. Some standardized in-vitro techniques are applied in cell culture models to determine effects of herbal extracts. These methods are based on extraction of active compounds from herbs, are then incubated in different cell lines in various dilutions. After exposition of cells, herbal extracts cytotoxicity is assessed using methods like microscopic cell morphology evaluation, methyl tetrazolium assay, lactate dehydrogenase activity. Such studies are widely accepted as most effective tests in pharmaceutical development. Same way some in-vivo techniques are also applied to test herbal drugs in order to get their physiological microenvironment. Conclusion: Systematic nomenclature, good quality control and accurate identification of herbal drugs play important concerns for scientific research. It is true that the quality of experimental studies and clinical trials on herbal drugs cannot be compared to the studies required by the FDA for a new drug approval; however, by experience, education and quality control about herbal drugs would make consumers, physicians and researchers more confident of the use of herbal drugs. Keywords: Herbal drugs, cell culture models, toxicity studies P-016: EVALUATION OF DIURETIC POTENCY OF ROOT EXTRACT OF HEMIDESMUS INDICUS Rathod M. L., Sheth N. R., Dudhrejia A. V., Parmar S. K. 1B.K.Mody Govt. Pharmacy College, Rajkot; 2Dept of Pharmaceutical Sciences, Saurashtra University The plant Hemidesmus indicus syn. Sariba, Family- Asclepiadaceae, is a perennial, slender, laticiferous, prostrate or semi-erect, twining, wiry shrub. Root part of this plant is commonly used as tonic, alternative, blood purifier, diaphoretic, diuretic and skin diseases. Crude hydro ethanolic extract of root of Hemidesmus indicus (CHEERHI) was evaluated for its diuretic activity by Lipschitz model in a dose of 250 and 500 mg/kg body weight by oral route. CHEERHI showed significant (p<0.01) increase in urine volume along with increased excretion of sodium, potassium, creatinine and chloride ions as compared to control group. CHEERHI showed delayed diuretic effect (after 4 hours of administration) as compared to furosemide. Thus, present study suggests that CHEERHI have diuretic potency. For further authenification of its exploration as diuretic drug it requires to conduct sub-acute and chronic experiments in rats for in vivo screening of diuretics. Keywords: Hemidesmus indicus, furosemide, Lipschitz model, metabolic cage P-017: ANTI-ULCER ACTIVITY OF ANNONA RETICULATA SEED MUCILAGE Roshan Patel, Naveen K. Mahobia, Nitinkumar Upwar, Naheed W. Sheikh, Sudarshan Singh Shree Leuva Patel Trust Pharmacy Mahila College, Amreli, Gujarat The plant Annona reticulata is also referred as custard apple which is widely cultivated in India for its edible fruit belongs to family Annonaceae. The seed bark contains useful tannins and astringents. The seed of Annona reticulata swells and form gelatinous mass when it comes in contact with water due to its hydrophilic nature. In present study anti-ulcer effect of seed mucilage of plant is evaluated by using ethanol induced ulcer in rats. Preliminary phytochemical screening shows the presence of only carbohydrate in mucilage. The degree of erosion was calculated in terms of ulcer index, which was scored by a person unaware of the experimental protocol, based upon the product of length and width of the ulcers present in the glandular portion of the stomach (mm2/rat). The results clearly revealed that the pre-treatment with mucilage (100-400 mg/kg, p.o.) have dose dependent anti-ulcer activity which is comparable with standard (Sucralfate).It was concluded that the finding results is may be due the formation of protective layer of mucilage against the ulcer inducer. Keywords: Annona reticulata, mucilage, anti-ulcer P-018: STANDARDIZATION AND EVALUATION OF THE POLYHERBAL COUGH SYRUP Nikunjana Patel, Jitendra Modi S K Patel College of Pharmaceutical Education & Research, Ganpat University, Kherva Zeal cough syrup (ZCS) is a liquid polyherbal formulation containing trikatu, tulsi, vasaka, yashtimadhu, sunthi as major drugs and used in respiratory diseases like cough and asthma. The syrup was provided by Vasu Health Care Pvt Ltd., Vadodara. The formulations from three different batches were evaluated for physicochemical parameters like viscosity, pH, specific gravity, sugar content, heavy metal residue, microbial contamination and quantification of major phytoconstituents by HPLC. Comparative TLC fingerprinting was also developed for raw material with syrup. The ZCS was also evaluated for acute toxicity and antitussive activity in sulphur dioxide induced cough model in mice using the codeine and chlorpheniramine maleate as a standard drug. The numbers
of bouts were measured as a parameter of evaluation and the % inhibition of cough bouts was calculated. The green color ZCS had pH 5.92 ±0.258, viscosity (162.67±0.543 poise), specific gravity (1.445±0.217), ammonium chloride (99.66±0.088), total sugar (40.32±0.606) and reducing sugar (15.80±0.176). The ZCS passes the tests of microbial contamination and heavy metal analysis. Comparative HPTLC fingerprint showed the presence of major phytoconstituents in raw materials as well as ZCS. The amount of piperine and glycyrrhizin were 8 mg/ml and 2.30 mg/ml respectively. In acute toxicity study the ZCS was found to be safe at 5000mg/kg body weight. The group of animals receiving standard drug significantly (84.397±0.887) reduced the number of bouts, while in case of ZCS low dose failed to produce significant inhibition. The higher dose of ZCS (0.5ml/kg) reduced the bouts significantly as compared to control group. Finally it can be concluded that there was very less batch to batch variations in physicochemical parameters with good antitussive activity without any toxicity. Keywords: standardization, polyherbal syrup, piperine, glycyrrhizin, HPLC P-019: LIQUORICE AUGMENTS PROTECTIVE ACTION OF VASAKA AGAINST CIGARETTE SMOKE INDUCED PULMONARY INFLAMMATION Manek RA1, Sheth NR2, Chavda JR1, Vaghasiya JD, Malaviya SV 1B K Mody Govt. Pharmacy College, Rajkot; 2Dept of Pharm. Sciences, Saurashtra Uni., Rajkot Context: Liquorice and Adhatoda vasica (family: acanthacea) are present in a number of herbal formulations for inflammation, including asthma. Both are known to possess protective efficacy against such types of disorders but their effect on pulmonary inflammation caused by cigarette smoke is unknown. Aim: The present study evaluated the effect of both plants individually as well as in combination on a rat model of cigarette smoke induced acute lung inflammation. Material and Methods: The albino rat groups orally received hydro-alcoholic (80%) extract of Adhatoda vasica AVE(100mg/kg), Glycyrrhiza glabra LE (250mg/kg), and AVE(100mg/kg)+LE(250mg/kg) 1 h before exposure to 20 commercial nonfiltered cigarettes per day for 11 days. Twenty-four hours after the last exposure, the animals were sacrificed and lung inflammatory responses were measured. Results: Treatment with AVE, LE and LE+AVE caused significant (p<0.001) reduction in % of neutrophils and CINC-1 levels in BALF as well as lung tissue TNF- as compared to CS challenged rats. Histology of LE, AVE and AVE+LE treated groups challenged with CS exhibited significant reduction in cellular infiltration, edema and showed intact alveolar architecture. Treatment with LE, AVE and LE+AVE significantly (p<0.001) restored the levels of redox cycle components melonaldehyde, reduced Glutathione, Super oxide dismutase and catalase activity in lung tissue of rat experimental model. Conclusion: LE+AVE effectively attenuated CS-induced pulmonary inflammation through inhibition of inflammatory cells infiltration, decreased oxidative stress, and reduction of pro-inflammatory mediator expression in lung, providing with the potential rationale for development of anti-inflammatory combination. Keywords: Glycyrrhiza glabra, Ardusi, herb-herb interaction P-020: PHARMACOGNOSTICAL STANDARDIZATION OF VOLATILE OIL AND DETERMINATION OF ORTHOSIPHON THYMIFLORUS Krunal P Jasani, Parth M Dave, Kaushik M Bhimani, Yogesh V Ushir, K R Vadaliya Shree H. N. Shukla Institute of Pharmaceutical Education and Research, Rajkot, Gujarat Orthosiphon thymiflorus (Lamiaceae) is popular garden plant in India which was collected from Jejuri,taluka;Purender,district: Pune, Maharastra state and it was authentificated from NISCAIR, New Delhi.The study conducted with the aim of drawing the pharmacognostical standardisation and determination of volatile oil of crude drug. By the hydrodistillation process volatile oil was separated out from orthosiphon thymiflorus and the physical parameters like solubility, specific gravity, optical rotation was determined, TLC pattern for the monoterpene like limonene, pinene was also studied. The essential oil of this drug may have various medicinal uses like antimicrobial & anti-inflammatory activity.Then, the determination and confirmation of volatile chemical constituents was carried out by gaschromatography-mass spectroscopy (GCMS). Which provides the prediction for pharmacological activity because of chemical constituents present in essential oil responsible for activity. No doubt the further investigation can be carried out for further determining different pharmacological activity and medicinal use of orthosiphon thymiflorus because of chemical constituents and metabolites present in essential oil. But at this level the present poster set up standardization parameter for orthosiphon thymiflorus. Keywords: (GC), (MS), orthosiphon thymiflorus, Lamiaceae P-021: ISOLATION AND DETERMINATION OF TRITERPENOID FROM ROOTS OF HYPTIS SUAVEOLENS Kaushik M Bhimani, Krunal P Jasani, Parth M Dave, Yogesh V Ushir, K R Vadaliya Shree H. N. Shukla Institute of Pharmaceutical Education and Research, Rajkot, Gujarat Hyptis suaveolens (Lamiaceae)commonly called as "CURRY LEAF" is a perennial, erect, annual and aromatic herb that may grow up to height of 2m. Wilayti tulsi named Hyptis suvaveolens were collected from roadside of Lalpari Lake, district:-Rajkot (Gujarat state) and it was authentificated from NISCAIR, New Delhi. Methanolic extract fractionisation was carried out in chloroform. From chloroform fraction triterpenoid was isolated by column chromatography. Triterpenoid identification did via chemical test, physical properties, TLC pattern. Then, the confirmation of chemical constituents was carried out by instrumental techniques as; UV, IR, MS and NMR. The pentacyclic triterpenoid stigmasterol structure interpreted at lastely. The ethanobotanical survey claim the valuable plant used for treating inflammation and rheumatoid arthritis (RA), No doubt, the further investigation must be needed for determining different pharmacological activity and medicinal use of Hyptis
suaveolens. But, at this level the present poster claim that the stated activity is because of stigmasterol, because in inflammation and RA the steroids and triterpenoids play a vital role. Keywords: Hyptis suaveolens, fractionisation, Triterpenoid, inflammation P-022: IN-VITRO ANTI-ARTHRITIC ACTIVITY OF HOLOPTELIA INTEGRIFOLIA LEAVES Dharmeshkumar Prajapati, N. M. Patel Shri B. M. Shah College of Pharmaceutical Education & Research, Modasa, Gujarat The World Health Organization (WHO) estimates 80% of the world population presently use herbal medicine for some aspects of primary health care. Rheumatoid arthritis (RA) is a chronic, systemic inflammatory disorder that may affect many tissues and organs, but principally attacks synovial joints. There are many herbs which are described in Ayurveda for arthritis. The medicinal plant contains flavonoids, terpenes, quinones, catechins, alkaloids, anthocyanins and anthoxanthins phytoconstituents, having anti-inflammatory effects. Holoptelea integrifolia belongs to the family of Urticaceae. The bark and leaves are bitter, astringent, anthelmintic, diabetes, helminthiasis, skin disease, intestinal disorder, leprosy, rheumatism. Alcohol, Water, succesive extracts of Petroleum ether, Chloroform, Methanol and Water extracts of H. integrifolia leaves were tested for Antiarthritic activity using Diclofenac sodium as standard by In vitro models like, Inhibition of protein denaturation and effect on membrane stabilization. Alcohol, Water and succesive Methanol extracts of H. integrifolia leaves exhibited significant Antiarthritic activity. The results of the investigations justify us the folklore use of H. integrifolia leaves in the treatment of inflammation and the plant is worth for further chemical isolation and pharmacological investigations. Keywords: Holoptelea integrifolia, Antiarthritic P-023: STANDARDIZATION OF PIPERINE CONTAINING HERBAL FORMULATIONS BY MODERN ANALYTICAL TECHNIQUES Yogita L. Makasana, Yogesh V.Ushir, K. R. Vadalia Shree H.N.Shukla Institrute of Pharmaceutical Education & Research, Rajkot, Gujarat Many of the pharmaceutical company involved in manufacturing Ayurvedic and herbal formulations, fails to full-fill the quality standards set up by Central Council for Research in Ayurveda. In present poster attempt were carried out on targeting piperine containing crude drugs as; pipali and marica available in some herbal formulation. Piperine is a good penetration enhancer as well as it increases the bioavailability of other drugs. The very popular formulations Sitopladi churna used in respiratory disorders and Abhayagi Modak tablet for laxative were purchased from local market of Rajkot. Both contain pipali and marica, these drugs helps to increase the action of other ingredients. Using modern analytical techniques here standardizations were conducted to achieve the goal, finding availability of pipali and marica qualitatively as well as quantitatively. The formulation evaluated by modern sophisticated analytical instruments like UV-Visible, HPTLC etc. The results obtained indicate that the herbal formulations content the pipali and marica but not as stated quantity which find on container and tablet strip. Keywords: Abhayagi Modak tablet, Bioavailability, HPTLC, Piperine, Sitopladi churna P-024: PERCEPTION OF MALAYSIAN MEDICAL STUDENTS ABOUT COMPLEMENTARY AND ALTERNATIVE MEDICINES Rajashekar Rao Barkur1*, Amardeep Singh2, Koh Huah Chian2, Teo Siok Har2 1Melaka Manipal Medical College, Manipal, Karnataka; 2Medical students, Melak Back ground- Complementary medicine and Alternative Medicines (CAM) refer to a group of therapeutic and diagnostic disciplines that exist largely outside the mainstream therapies that are not typically taught in medical school or generally available in hospitals. The Modes of CAM IncludeAcupuncture, Traditional Chinese medicine, Herbal medicine, Tai Chi Massage, Reflexology, Qigong, Yoga, Chiropractic and Ayuverdic medicine. Objectives and Methods-In this study using questionnaire, we assessed the knowledge, attitude and perception of Malaysian medical students (n=405) towards complementary and alternative medicines. Result and conclusion- The survey showed that majority of medical students believed that some of the CAM modalities were useful. Medical students also indicated that they have a good knowledge of massage therapy, acupuncture, herbal medicine and yoga practices. But students were not sure about Chiropractic and Qi Gong practices. Knowledge of commonly held beliefs about CAM modalities was generally poor, even for modalities which students claimed to know most about. Majority of medical students (74%) felt that clinical care should integrate the best of conventional and CAM practices. Medical student (75%) also claimed that knowledge about CAM is important for them as a future practicing health professional. Keywords: Complementary and Alternative Medicines P-025: PHARMACOGNOSTICAL AND PRELIMINARY PHYTOCHEMICAL PROFILING OF COLEUS AROMATICUS BENTH. Shahin khan*, Kishor Dholwani, Ajay Saluja A.R.College of Pharmacy & G.H.Patel Institute of Pharmacy, V.V.Nagar, Gujarat Coleus aromaticus belonging to the family Lamiaceae commonly known as Pattaajwain, Pattharchur in Hindi, Karpuravalli, Omavalli in Tamil, Country borage in English is a large succulent aromatic perennial herb. It is highly aromatic pubescent herb with distinctive smelling leaves. Much branched, fleshy highly aromatic pubescent herb with distinctive smelling leaves. It is a folkloric medicinal plant used to treat malarial fever,
hepatopathy, renal and vesical calculi, cough, chronic asthma, hiccough, bronchitis, helminthiasis, colic, convulsions and epilepsy. It can be called as complete winter herb. Inspite of its wide traditional uses, it is very rarely or not seen in market formulation of renal stone or cold preparation.Various studies on sub-acute toxicity reveals that no mortalities or evidence of adverse effects have been observed in Albino mice following acute oral administration at the highest dose of 2000mg/kg crude extracts of C. aromaticus.The leaf of C. aromaticus was collected from the Mehsana, Gujarat. Various pharmacognostical and preliminary phytochemical analysis and TLC studies was carried out. The outcomes are then compared with the reported data. Lack of proper standards of medicinal plants may result in the usage of improper drugs which in turn will cause damage not only to the individual using it, but also to the respect gained by the well-known ancient system of medicine. Therefore scientific method must be resorted to identify and maintain quality of plant drugs to be used in the traditional system of medicine. Keywords: Coleus aromaticus, TLC P-026: PRELIMINARY PHARMACEUTICAL CHARACTERIZATION OF CASSIA AURICULATA FLOWER AS POTENTIAL PHARMACEUTICAL DYES: ACID-BASE INDICATOR Parth M Dave, Krunal P Jasani, Kaushik M Bhimani, Sudarshan singh, K R Vadaliya Shree H. N. Shukla Institute of Pharmaceutical Education and Research, Rajkot, Gujarat Cassia auriculata is a species of S. auriculata belonging to family Fabaceae. The present vocation highlights the exploit of the acidified ethanolic extract of the flower as an acid-base indicator in acid-base titrations. This natural indicator is easy to extract as well as easily available. The titration was performed for strong acid and base, weak acid and base, strong acid and weak base and weak acid and strong base. Promising results were obtained when it was tested against standard synthetic indicators. Titration shows sharp color change at the equivalence point. The equivalence points obtained by the flower extract coincide with the equivalence points obtained by standard indicators. In case of weak acid and weak base titration, the results obtained by the flower extract matched with the max was found to be 256.5nm and foundresults obtained by mixed indicator. The to be chemically stable. This natural indicator is found to be a very useful, economical, simple and accurate for the said titrations. Keywords: Cassia auriculata, fabaceae, titration, flower extract P-027: IN-VITRO ANTI-OXIDANT ACTIVITY OF GMELINA ARBOREA FRUITS PERICARP EXTRACTS D. L. Chothani*, T. Y. Pasha, N. M. Patel Pioneer pharmacy Degree College, Vadodara, Gujarat The present study was aimed to evaluate antioxidant activity of various extract of fruits pericarp of Gmelina arborea (Verbenaceae). Antioxidant activity was evaluated by using DPPH free radical scavenging activity and reducing power by FeCl3. The methanolic extract (ME), water extract (WE) and ethyl acetate extract (EAE) were evaluated. The Ascorbic acid was used as positive control. The various extract showed a significant free radical scavenging activity when compared with standard. It is concluded that the Gmelina arborea fruits pericarp possess antioxidant activity. Antioxidant activity may be attributed to its phenolic and flavonoid compound. Further investigation of pericarp is suggested to isolate the active principle responsible for the activity. Keywords: Antioxidant activity, DPPH free radical scavenging activity, Gmelina arborea P-028: SYNERGISTIC ANTI-OXIDANT AND PRO-OXIDANT ACTIVITIES OF SOME INDIGENOUS HERBAL FORMULATIONS Archana Banerjee, Sainiara Begum, Swati Bhattacharya, Anandita Bandyopadhyay Department of Botany, Surendanath College, Kolkata In Bengal many plants are used in herbal drink formulations and possess health potential comparable with tea. Fruits of Averrhoa carambola L., Citrus spp., Dillenia indica L. (juice of ripe calyx), Emblica officinalis Gaertn., Hibiscus sabdariffa L. (ripe calyx), Mangifera indica L. (raw pulp), Pterospermum acerifolium (perianth), Spondius mangifera Willd., Syzygium cumini Skeels, Tamarindus indica L. are traditionally used as anti fatigue refreshing drinks. In a combination of five ingredients used in cold and cough the spice ingredients with aromatic leaves/fruit pulp exhibited synergistic antioxidant activity whereas the combination of Piper nigrum L. with Cinnamomum tamala Nees & Eberm exhibited pro oxidant activity. Highest total antioxidant activity was observed in raw M. indica drink synergistic with mustard seeds followed by E.officinalis >P.granatum >S. cumini >G. asiatica >H. sabdariffa >T. indica >S. mangifera >P. acerifolium>A.carambola. These are effective in the treatment of fever, diarrhea and dysentery, constipation, cardiovascular disease, cancer, inflammatory conditions, asthma, liver disease, diabetes, neurodegeneration and oxidant-antioxidant balance. Keywords: Health drink, spice, antioxidant synergy, pro oxidant P-029: LC-MS N-ALKYLAMIDE PROFILING OF AN ETHANOLIC ANACYCLUS PYRETHRUM ROOT EXTRACT Vikas Sharma, Jente Boonen, Bart De Spiegeleer, V.K. Dixit Department of Pharmaceutical Sciences, H.S. Gour University, Sagar, M.P. The roots of Anacyclus pyrethrum DC (AP) (Asteraceae) are frequently used in traditional medicine as Vajikarana Rasayana. An ethanolic extract of root of Anacyclus pyrethrum demonstrated its potential to enhance the sexual behaviour of male rats, with a dose dependent effect on sperm count and androgens concentration. Phytochemical analysis of ethanolic extract of Anacyclus pyrethrum revealed that it is rich in N-alkylamide. This
study therefore sought to assess characterization of ethanolic extract of Anacyclus pyrethrum root. Root extract was performed using a gradient reversed phase high performance liquid chromatography/UV/electrospray ionization ion trap mass spectrometry (HPLC/ESI-MS) method on an embedded polar column. MS1 and MS2 fragmentation data were used for identification purposes, while UV was used for quantification. Thirteen Nalkylamides (five N-isobutylamides, three N-methyl isobutylamides, four tyramides and one 2phenylethylamide) were detected. Five of them identified as undeca-2E,4E-diene-8,10-diynoic acid N-methyl isobutylamide, tetradeca-2E,4E-diene-8,10-diynoic acid tyramide, deca-2E,4E-dienoic acid N-methyl isobutylamide, tetradeca-2E,4E,XE/Z-trienoic acid tyramide and tetradeca-2E,4E,8Z,10Z-tetraenoic isobutylamide are novel compounds, which have never been identified in Anacyclus pyrethrum. Keywords: Anacyclus pyrethrum (Asteraceae), LC-MS plant profiling, N-alkylamides P-030: ANTIDIABETIC ACTIVITY OF ANNONA CHERIMOLA Chakrapani.P* and Anupalli Roja Rani Department of Genetics, Osmania University, Hyderabad, A.P. Diabetes mellitus is a group of metabolic disorders with one common manifestation hyperglycemia. Chronic hyperglycemia causes damage to eyes, kidneys, nerves etc. It is caused by inherited or acquired deficiency in production of insulin by the pancreas, or by the ineffectiveness of the insulin. Currently available synthetic antidiabetic agents like sulfonyl ureas, biguanides, etc being expensive produce serious side effects. Their use is not safe during pregnancy. Thus due to an increase in demand by patients to use natural products with antidiabetic activity, investigations on hypoglycaemic agents derived from medicinal plants have gained popularity in recent years. Medicinal plants are the development of alternative drugs and strategies for better management of diabetes. Annona is a genus of flowering plants in the pawpaw/sugar apple family, Annonaceae. Many of the annona species are used in traditional medicines for the treatment of diseases. The present study the leaf alcohol extract of the plant Annona cherimola (A. cherimola) was investigated for its antidiabetic activity in streptozotocin (STZ) induced diabetes mellitus in rats. Graded doses of the leaf alcohol extract were administered to normal and experimental diabetic rats for 30 days. Fasting plasma glucose levels, serum insulin levels, serum lipid profiles and changes in body weight were evaluated in normal rats while liver glycogen levels and pancreatic lipid peroxidation levels were evaluated additionally in diabetic rats. The findings showed the significant antidiabetic potential of the extract in ameliorating the diabetic conditions in diabetic rats. No significant effects were found in the normal rats. Keywords: Diabetes ,Annonaceae, streptozotocin , Annona cherimola, hypoglycaemic P-031: STUDY OF A MIRACLE ALOE AN IMPORTANT MEDICINAL PLANT FOR ANTIDIABETIC ACTIVITY AND HISTOPATHOLOGY IN STREPTOZOTOCIN INDUCED DIABETES B. Chandra Sekhar Singh* and Anupalli Roja Rani Department of Genetics, Osmania University, Hyderabad, Andhra Pradesh Diabetes is characterized by hyperglycemia with biochemical alterations of glucose and lipid metabolism. The association of hyperglycemia with alterations of lipid parameters presents a major risk for cardiovascular complications. Over a 13 year period from 1997 & 2010 the number of people worldwide, suffering with diabetes has doubled. Ethnobotanical information indicates that more than 800 plants are used as traditional remedies for the treatment of diabetes. Aloe vera belongs to the family Liliacae with 360 species. The most popular is Aloe barbadensis Miller which has most therapeutic value. Aloe is used extensively in the cosmetic and pharmaceutical industry. Researchers isolated a number of active phytosterol compounds from the gel that were found to reduce blood glucose. These compounds were evaluated for their anti-hyperglycemic effects in type 2 diabetes. We studied the beneficial effects of Aloe vera (CIM-sheetal) in streptozotocin induced diabetic rats. In diabetic induced rats fed with Aloe CIM-sheetal powder (350 mg/kg body wt), the fasting plasma glucose levels were reduced to normal and body weight was found to be increased. In the pancreatic sections of diabetic rats fed with Aloe CIM-sheetal, the islets were comparable to normal rats. In liver, the changes caused after induction of diabetes are granular cytoplasm, dilated sinusoids, shrunken nuclei and inflammation, which was reduced after feeding with Aloe CIM-sheetal. In diabetic rats and diabetic rats fed with Aloe CIM-sheetal, no change was noticed in the kidney. The effective role of Aloe species is to obtain novel drugs in the management of diabetes mellitus. Keywords: Aloe CIM-sheetal, phytosterol, Diabetes, streptozotocin, lipid peroxidation P-032: ANTICARIOGENIC ACTIVITY OF CALOTROPIS SP. PLANTS LATEX AND STRUCTURE ELUCIDATION OF BIOACTIVE COMPOUND Kalpesh Ishnava, Jenabhai B Chauhan, Akanksha A Garg and Arpit Thakkar ARIBAS, Sardar Patel University, New Vallabh Vidyanagar, Gujarat Dental problem is the most common health problem in the human communities. Microorganisms in mouth can also cause other infection. According to latest WHO report on dental disorders, affects about 60% of the world adult population. Despite the advances concerning its prevention and control, dental caries is still considered a public health problem that affects many countries in the world. In the present study, in vitro anticariogenic potential of the chloroform, ethyl acetate, hexane and methanol and aqueous extracts of Calotropis gigantia (L.) R. Br. and Calotropis procera (Ait.) R.Br. was evaluated by using five cariogenic bacteria, Actinomyces viscosus, Lactobacillus acidophilus, Lactobacillus casei, Streptococcus mitis and Streptococcus mutans. Agar well diffusion method and minimum inhibitory concentration (MIC) were used for this purpose. The chloroform extracted
fraction of latex showed good inhibitory effect against all selected organic solvent. In Calotropis gigantia chloroform extracts, S. mutans and L. acidophilus with MIC value of 0.032 and 0.52 mg/mL, respectively. The data of Calotropis procera showing less active compare to Calotropis gigantia. Latex : DMSO (1:1) and Latex : Distilled water (1:1) against maximum activity showing in the Streptococcus mutans (32 mm). Qualitative investigation on structure elucidation of bioactive compound using IR, NMR and GCMS techniques revealed the presence of methyl nonanoate, a saturated fatty acid. Plant extracts have great potential as anticariogenic that may be useful in the treatment of dental caries. P-033: ANTILITHIC ACTIVITY OF BOERHAAVIA DIFFUSA LINN, COMMIPHORA WIGHTII AND ROTULA AQUATICA LOUR ON URINARY TYPE STRUVITE CRYSTALS C. K. Chauhan1*, M. J. Joshi1, A. D. B. Vaidya2 1 Dept. of Physics, Saurashtra University, Rajkot; 2Kasturba Health Society, ICMR Advanced Center Struvite-(NH4) MgPO4.6(H2O), ammonium magnesium phosphate hexahydrate is one of the components of urinary calculi. Struvite calculi can grow rapidly forming "staghorn-calculi", which is more painful urological disorder with life-threatening complications. It has also high degree of recurrence. Epidemiological studies from various countries continue to report a frequency of the occurrence of struvite stones between 25 % and 38 %. Although, surgical management has become increasingly tolerable, the inhibition and medical prevention of recurrent struvite calculi is greatly desirable. It is the need of society to discover such drugs, which can inhibit struvite growth, in addition to high success rates, excellent safety profile and low side effect profile. In vitro single diffusion gel growth technique was used to study the growth-inhibition behavior of struvite crystals by using the herbal extracts like Boerhaavia diffusa Linn, Commiphora wightii and Rotula aquatica Lour. Sodium Metasilicate (SMS) solution of specific gravity 1.05 was used to prepare the gel. An aqueous solution of ammonium dihydrogen phosphate of 0.5 M concentration was mixed with the SMS solution in appropriate amount to set the pH value 7.0. After the gelation took place, 20 mL supernatant solutions of pure 1.0 M magnesium acetate (control solution), and 1.0 M magnesium acetate prepared with 0.5% and 1.0% concentrations of the each of the herbal extracts were gently poured on the set gels in test tubes in the aseptic medium. It was observed that the number, dimensions, total mass, total volume, growth rate and depth of growth of struvite crystals decreased with the increasing concentrations of each herbal extract. The enhancement of dissolution rate and fragmentation of struvite crystals were also noticed. All the investigated herbal extracts are found to have antilithic activity and proved as potent inhibitors for struvite crystals. These results are important from the reverse pharmacology point of view. Keywords: Struvite, urinary calculi, gel growth, inhibition, and herbal extracts P-034: ANTI-ARTHRITIC ACTIVITY OF ALCOHOLIC AND WATER EXTRACT OF AN AYURVEDIC FORMULATION PANCASAMA CHURNA BY FREUND'S ADJUVANT INDUCED ARTHRITIS RATS MODEL Hardik P. Patel*, Nimisha D. Darji S. K. Patel College of Pharmacetical Education & Research, Ganpat Universtiy, Kherva, Gujarat Objective: The aim of study was to evaluate the anti-arthritic activity of the water and alcoholic extract of an Ayurvedic formulation Pancasama churna which contains (Zingiberofficinale, Terminelia chebula, Piper Longum, Operculina turpethum, Saindhav salt). Pancasama Churna is one of the classical Ayurvedic dosage form mentioned in Ayurvedic formulary of India for Udara roga (stomach disease), Adhamana (flatulence with sound), Sula (pain), Amavata (Rheumatism), Arsa (piles). Methods: The anti-arthritic activity of Pancasama churna were carried out by Freund's adjuvant induced rats arthritis model after approval of Institutional Animal Ethical Committee. Water and Alcoholic extracts of Pancasama churna 100 mg/kg and 200 mg/kg, 1 % Carboxymethyl cellulose was used as vehicle control, dexamethasone 1 mg/kg positive control given orally to rat. Anti-arthritic activity was evaluated by determining physical parameters (Inflammation, mobility test, Gait test, Joint stiffness, joint hyperalgesia), hematological parameters (red blood cells (RBC), white blood cells (WBC) and hemoglobin), and biochemical parameters (Erythrocyte Sedimentation rate (ESR) and Rheumatoid Arthritis Factor (RA factor). Results and Discussion: Results of anti arthritic activity of alcoholic and water extract of pancasama churna were significant as compared to vehicle treated control groups. Significantly decrement of inflammation of rat paw volume (p<0.05 to p<0.001), Gait score(p<0.05 to 0.001), WBC (p<0.05 to p<0.001), rheumatoid arthritis (RA) factor (p<0.001 in all treatment groups), and erythrocyte sedimentation rate (ESR), significantly (p<0.05 to p<0.001), RBC (p<0.001 in all treatment groups), and hemoglobin (p<0.05 to p<0.001), significantly in treated groups from 0 to 28 days. Both alcoholic and water extract of pancasama churna shows significant anti arthritic activity as compare to control. Conclusions: Pancasama churna contain piperine, embelin and carvone phytoconstituents in their plant raw materials. These constituents may be responsible for significant anti arthritic activity of this churna. Keywords: Anti-arthritic activity, Pancasama churna, Freund's adjuvant induced P-035: EVALUATION OF ANXIOLYTIC ACTIVITY OF VARIOUS EXTRACTS OF AERIAL PART OF CLEOME GYNANDRA Patel T. D, Mishra S.H, Dodia R. A, Pancholi S.S. Dept. of Pharmacognosy, Babaria Institute of Pharmacy, Varnama, Vadodara To date, the search for novel pharmacotherapy from medicinal plants for psychiatric illnesses has significantly progressed. The present study was performed to evaluate the anxiolytic activity of various extracts viz.
petroleum ether, chloroform and methanol extract of aerial part and methanol extract of root of Cleome gynandra using elevated plus maze (EPM) model in mice. Mice were treated orally with different doses of the extracts (i.e. 50, 100 and 200 mg/kg) and behavior was observed on the elevated plus maze. The methanol extract of aerial part of Cleome gynandra at the dose of 200 mg/kg increased the percentage of time-spent and percentage of entries in the open arms of elevated plus maze. The anxiolytic activity may be due to presence of saponins. Hence this plant may be developed as a potentially useful anti anxiety agent. Keywords: Anxiolytic, Elevated plus maze, saponin, Cleome gynandra. P-036: EVALUATION OF DIURETIC ACTIVITY OF AQUEOUS AND ETHANOLIC EXTRACTS OF COMMELINA DIFFUSA BURM F. IN RATS Jigar B. Vansola, Ankita J. Patel, Nikunjana A. Patel, Rakesh K. Patel S. K. Patel College of Pharmaceutical Education and Research, Ganpat University, Kherva, Gujarat The present study was undertaken to investigate diuretic effect of aqueous and ethanolic extracts of the stem and leaves of Commelina diffusa burm f. in rats. Aqueous and ethanol extracts of Commelina diffusa burm f. were administered to experimental rats orally at doses of 200 and 400 mg/kg p.o. Hydrochlorothiazide (10 mg/kg) was used as positive control in study. The diuretic effect of the extracts was evaluated by measuring urine volume, sodium and potassium content. Urine volume was significantly increased in ethanol extracts of Commelina diffusa burm f. leaves with comparison to control group and the excretion of sodium and potassium was also increased in same extract of Commelina diffusa burm f. leaves. While there is no significant effect of aqueous extracts of leaves and stem. The diuretic effect of the extracts was comparable to that of the reference standard (hydrochlorothiazide). So it can be conclude that the ethanol extract of Commelina diffusa burm f. leaves produced notable diuretic effect which appeared to be comparable to that produced by the reference diuretic hydrochlorothiazide. The present study provides a quantitative basis for explaining the folkloric use of Commelina diffusa burm f. as a diuretic agent in Chinese population. Keywords: Diuretic activity, Commelina diffusa, hydrochlorothiazide P-037: IN-VIVO AND IN-VITRO IMMUNOMODULATORY ACTIVITY OF METHANOLIC EXTRACTS OF SEEDS AND BARK OF PONGAMIA GLABRA VENT. Sanjeev Heroor*, Arunkumar Beknal, Nitin Mahurkar HKES's MTR institute of Pharmaceutical Sciences, Gulbarga, Karnataka Objective: To evaluate immunomodulatory activity of methanolic extracts of seeds and bark of Pongamia glabra Vent. on cyclophosphamide induced myelosuppression in mice and the phagocytic effect on human neutrophils. Materials and Methods: Methanolic extracts of seeds and bark of Pongamia glabra Vent. (500mg/kg p.o.) were administered for 13 days to albino mice and cyclophosphamide (30mg/kg i.p.) was administered on 11th,12th and 13th days 1 hour after the administration of the respective treatment. On the 14th day blood collected by retro-orbital puncture and the activity was evaluated by determining the RBC, Hb%, platelet, total WBC and differential counts. The two extracts of the plant in the concentration range 100,50,25,12 and 6.25 µg were also subjected to evaluate the phagocytic effect on human neutrophils using the in-vitro models nitroblue tetrazolium (NBT) dye test, phagocyotosis of Candida albicans and chemotaxis assay. Results: Methanolic extracts of seeds and barks of Pongamia glabra Vent. showed highly significant counteracting effect (p<0.001) to cyclophosphamide induced reduction in total WBC, DLC and platelet counts and significant (P<0.01) effect to that of reduction in RBC counts and Hb %. The extracts of the plant in the concentration range 100,50,25,12 and 6.25 µg also showed significant (P<0.01) phagocytic effect on human neutrophils in the parameters studied. Conclusion: Methanolic extracts of seeds and barks of Pongamia glabra Vent. exhibited immunostimulant property in both in-vivo and in-vitro models. Keywords: Pongamia glabra, cyclophosphamide, immunomodulatory, NBT, Candida albicans P-038: ESTIMATION OF ANDROGRAPHOLIDE IN HEPATOPROTECTIVE FORMULATIONS USING HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHIC TECHNIQUE C.P. Jatav, P.R. Itankar, A.T. Patil Dept. of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University Campus Andrographolide is the active diterpene lactone obtained from Andrographis paniculata Nees (Family Acanthaceae). This compound offers a significant hepatoprotection againts D. Galactosamine, Paracetamol and Carbon tetrachloride. In the present study, accurate, simple, specific and reproducible HPTLC methods have been developed and validated. The sample extracts and standard marker were applied on the HPTLC silica gel 60 F 254 plates (E. Merck, Germany ) using a CAMAG Linomat IV applicator (Switzerland). The plates were developed with a twin-trough developing chamber. After development, the plates were scanned with a CAMAG TLC scanner V at 223 nm and the data were processed with WinCATS software. The HPTLC method developed was applied for three different concentration of isolated methanolic extract of A.paniculata herb samples, and two different marketed formulations containing A. paniculata as major constituent. Andrographolide appeared at retardation factor, Rf 0.7 in a methanolic extract of A. paniculata with a Chloroform: Methanol (70:10) solvent system. Keywords: Andrographolide, Hepatoprotective, HPTLC, Andrographis paniculata P-039: EVALUATION OF ANALGESIC, ANTI-INFLAMMATORY AND ANTI-ARTHRITIC ACTIVITIES OF AQUEOUS EXTRACT OF AERIAL PART OF ERYTHRINA INDICA
Hardik Ghelani, Sushil Suthar, Purnima Ashok, Ashish Kyada, Pinakin Jadav, Manish Rachchh Department of Pharmacology, S.J.Thakkar Pharmacy College, Rajkot Non-steroidal anti-inflammatory drugs are capable of relieving pain and reduce inflammation effectively but are associated with serious side effects such as gastric hemorrhage, ulceration, liver and kidney toxicities. Therefore, the development of new analgesic and anti-inflammatory drugs with fewer side effects become an urgent need. The aim of the present study was to investigate analgesic, anti-inflammatory and anti-arthritic activities of aqueous extract of aerial parts of Erythrina indica (AEEI). AEEI was evaluated for both peripheral (acetic acid-induced writhing) and central analgesic activities (tail immersion and hot-plate methods). The antiinflammatory activity was evaluated against carrageenan induced acute paw inflammation, cotton wool granuloma method and Complete Freund's Adjuvant (CFA) induced arthritis models. Various biochemical parameters like hydroxyproline and total protein content in CFA induced edematous tissue and haematological parameters like total RBC count, total WBC count, percentage of lymphocytes and percentage of neutrophils were also estimated at the end of study period. The AEEI exhibited significant (P < 0.05) analgesic, antiinflammatory and anti-arthritic activities on experimental animals and the results were comparable to those observed with standard drugs. The analgesic and anti-inflammatory actions of AEEI can be attributed to its active constituents such as alkaloids, flavonoids and tannins which are known to act through inhibition of prostaglandins biosynthesis. Keywords: Analgesic, Anti inflammatory, Anti Arthritic, Erythrina indica P-040: ANXIOLYTIC EFFECT OF LAGENARIA SICERARIA FRUITS ON THE ELEVATED PLUS MAZE MODEL OF ANXIETY IN RATS Rakesh Prajapati1, Manisha Kalariya2, Sachin Parmar2, Navin Sheth2 1Bhagwan Mahavir College of Pharmacy, Surat; 2Dept of Pharm. Sciences, Saurashtra Uni., Rajkot Context: In current era of globalization, approximately one-eighth of the total population of the world is affected with anxiety and other neurological disorders. Lagenaria siceraria (Molina) Standley (LS), commonly known as `bottle gourd' (English), possesses several medicinal properties; little is known about its traditional use as nerve tonic. Objective: The purpose of the study was to evaluate the anxiolytic activity of methanolic extract of Lagenaria siceraria (Molina) Standley fruits (MLSF) using elevated plus maze model. Materials and methods: Adult Wistar albino rats were subjected to elevated plus maze (EPM) test. Diazepam (Benzodiazepines) was used as reference standard drug. Results and Discussion: The anxiolytic activity of MLSF (100, 200, and 400 mg/kg) per os (p.o.) was characterized by increased time spent and number of entries in open arms in the EPM paradigm as compared to control group (p < 0.001). The phytochemical screening revealed the presence of flavonoids, saponins and sterols in the fruits. Conclusions: The results of the study for the first time show that the plant possesses anxiolytic activity, confirming the traditional claims. Future research should focus on the isolation and identification of the phytoconstituents responsible for the anxiolytic activity of LS. Keywords: Anxiety, Lagenaria siceraria, elevated plus maze model, phytochemical P-041: ANTI-INFLAMMATORY PROPERTIES OF THE LEAVES OF FICUS AMPLISSIMA SMITH. Arunachalam Karuppusamy Bioprospecting Laboratory, Department of Botany, Bharathiar University, Coimbatore, Tamil Nadu The Indian plant Ficus amplissima Smith belongs to family Moraceae, commonly known as kal-ittchi. The species is endemic to India and found everywhere in India. It commonly occurs in foothills of Himalaya, Assam, Sikkim, Kerala, Tamil Nadu, Andhra Pradesh, Maharastra. The ethnobotanical views on Ficus amplissima, suggest that fruits are chewed for mouth ulcers whereas leaf juice is applied externally in case of old chronic wounds. Leaf is boiled with salt and boiled rice water in equal proportion and blue warm solution is given in colic and also leaf is used for jaundice, fruit is used to cure mental deranged and the latex is applied on the wound. There was no scientific evidence justifying the use of Ficus amplissima, therefore the present study was aimed at evaluation of anti-inflammatory activity of the plant. In the present study the leaves of Ficus amplissima were studied for anti-inflammatory by using carageenan induced rat paw edema and histamine induced methods. F. amplissima leaf expressed the potent anti-inflammatory and antioxidant activity, acetone extract of (100mg) showed high activity. The acetone extract of F. amplissima leaf exhibited greater antiinflammatory, wound healing and antioxidant activity probably due to phenols constituents. Keywords: Ficus amplissima, Kal-ittchi, anti-inflammatory, carageenan P-042: PROTECTIVE EFFECT OF AERVA LANATA (L) ON DOXORUBICIN INDUCED CARDIAC APOPTOSIS IN WISTAR RATS Ragavendran P, Sophia D, Arul Raj C, Starlin T, Chella Perumal P and Gopalakrishnan V.K. Karpagam University, Coimbatore, Tamil Nadu Doxorubicin is an anticancer drug but it causes cardiac damage remain largely unknown to understanding the fundamental importance in doxorubicin induced cardiac toxicity. In this study, to evaluate the protective effect of Aerva lanata against doxorubicin induced cardiac apoptosis. Wistar rats used for this study. The animals were divided into 9 groups. Group I serve as control. Group II is doxorubicin induced cardiac toxicity groups (2.5mg/kg body weight). Groups III, IV and V are doxorubicin induced and treated with Aqueous, Ethanol and Aqueous ethanol extract of Aerva lanata (200mg/kg body weight for 28 days). Groups VI, VII and VIII are Aqueous, Ethanol and Aqueous ethanol extract of Aerva lanata alone. Group IX is doxorubicin induced and treated with standard drug aspirin (1.2mg/kg body weight for 28 days). After that, body weight and heart weight was noted. Animals were sacrificed heart was separated for biochemical estimations such as Collagen,
Proteolytic activity, Cytochrome b5 reductase, Cytochrome p450, Cathepsin D, Succinate dehydrogenase, NADH dehydrogenase, DNA were analyzed in tissue homogenate. Lipids were isolated from rat heart and analyzed for free fatty acid, Phospholipids and its types. The expression of calpain was analyzed by casein zymography method. The levels of Cytochrome b5, Cytochrome P450, Cathepsin D, Proteolytic activity, Free fatty acid, Phospholipids and its profiles were significantly increased in group II, after treatment with Aerva lanata the level was significantly decreased and similar to that of Group I. The level of Collagen, Succinate dehydrogenase, NADH dehydrogenase, DNA were significantly decreased in group II, after treatment with Aerva lanata the level was significantly increased and similar to that of Group I. In casein zymography of calpain analysis, Group I exhibit more expression when compared with group I, after the treatment with Aerva lanata inhibit the expression of calpain which is similar to that of control. Based on all these findings, Aerva lanata has ability to protect the cardiac cell death induced by doxorubicin and it may be good pharmacotherapeutic agents in future. Keywords: Doxorubicin, Cytochrome p450, Cytochrome b5, Cathepsin D, SDH P-043: PHYTOCHEMICAL SCREENING, ANTIOXIDANT ACTIVITY AND HPTLC ANALYSIS OF TYLOPHORA PAUCIFLORA WIGHT & ARN AN IN VITRO STUDY Starlin T, Ragavendran P, Arul Raj C, Chella Perumal P and Gopalakrishnan V.K. Karpagam University, Coimbatore, Tamil Nadu Plants having high content of antioxidants. The role of antioxidants is scavenging free radicals and protects the organs from oxidative stress. Plant based drugs produced less side effects when compared with allopathic drugs. Generally Tylophora genus has been used in the Ayurvedic system for the treatment of various diseases. In this study, to evaluate the phytochemical screening, antioxidant potential and HPTLC analysis of Tylophora pauciflora was investigated in this study. Based on the phytochemical screening ethanol extract was selected. The plant exhibited the most potent radical scavenging activity at a maximum concentration 2.5mg/ml. Natural antioxidants such as Flavonoid, Total phenols, Tannin, Carotenoids and Lycopene were evaluated and also the antioxidant activity against DPPH radical, Super oxide anion, Hydroxyl radical, Nitric oxide radical, Hydrogen peroxide radical, Total antioxidant capacity assay and anti-lipid peroxidation activity were evaluated and also compared with standards such as BHT, Vitamin C.. Tylophora pauciflora showed maximum anti lipid peroxidation against TBA. Strong antioxidant activity was observed in the ethanolic extract similar to standards ascorbic acid and BHT. Based on the phytochemical screening, constituents named alkaloid, flavonoid, phenols, terpenoid, tannins, glycosides estimated in ethanolic extract of Tylophora pauciflora. Based on this finding, Tylophora pauciflora had phytochemical constituents and also had free scavenging activity. This plant may be explored for therapeutic agent in future and used to treat against diseases which produced from oxidative stress. Keywords: Tylophora pauciflora, phytochemical screening, Natural antioxidants P-044: EVALUATION OF IN VITRO PHYTOCHEMICAL SCREENING, CYTOTOXIC ACTIVITY AND FTIR ANALYSIS OF ALPINIA PURPURATA (VIEILL) K. SCHUM Arul Raj C, Ragavendran P, Sophia D, Starlin T, Chella Perumal P and Gopalakrishnan V.K. Karpagam University, Coimbatore, Tamil Nadu Plants have been used in traditional medicine for several thousand years. Alpinia purpurata (vieill) K. Schum a popular ornamental plant located all over India. The phytochemical constituents of A. purpurata promote anti inflammatory activity, antidiabetic activity in Type II Diabetes mellitus and antimicrobial activity against certain microorganisms. The aim of the present study, to evaluate the In vitro phytochemical screening cytotoxic activity and FTIR analysis of A. purpurata. The ethylacetate extract of Alphinia Purpurata was checked for cytotoxic activity and FTIR analysis, based on phytochemical analysis. Ethylacetate extract showed most of the phytochemicals in the plant. Ovarian cancer cell line (PA-1) was treated with different concentration (6.25µg 400/ml) of ethylacetate extract of A. Purpurata and incubated for 12, 24, 48 and 72 hours. A. Purpurata treated PA-1 cells showed the IC50 value at 145µg/ml. The FTIR analysis showed the presence of major functional groups in the particular extract. It is further concluded that ethylacetate extract showed most of the phytochemicals in the plant. Cytotoxicity activity was checked with ethylacetate extract of ovarian cancer cell line (PA-1) and FTIR analysis showed the presence of functional groups in the extract. Further studies are in progress for the isolation of compounds and its anticancer activity in animal model. Keywords: Alpinia purperata, Phytochemical, FTIR, Cytotoxicity P-045: FREE RADICAL SCAVENGING ACTIVITIES AND HPTLC DETERMINATION OF CAYRATIA TRIFOLIA Chella Perumal P, Sophia D, Ragavendran P, Arul Raj C, Starlin T and Gopalakrishnan V.K. Karpagam University, Coimbatore, Tamil Nadu Bioactive compounds commonly found in edible plants such as fruits, vegetables, flowers, and leaves have been shown to confer health benefits. In the present study, the ethanol extract of the whole plant of Cayratia trifolia were evaluated for their free-radical scavenging capacity and their antioxidant activity by a number of chemical assays. The chemicals assays that were employed includes 2, 2- diphenyl-1-picryl-hydrazyl (DPPH), ferric reducing antioxidant power, superoxide radical scavenging, hydroxyl scavenging, nitric oxide scavenging and singlet oxygen scavenging activities. The results showed that the ethanol extract of Cayratia trifolia possess the free radical scavenging activities. The high performance liquid chromatography (HPTLC) analysis revealed the presence of alkaloids and flavonoids in the ethanol extract of Cayratia trifolia. Thus, the ethanol extract of Cayratia trifolia exhibited the highest antioxidant activity based on the tests performed and can be used as a
bioactive source of natural antioxidants and are potential natural resources for pharmacology of functional foods. Keywords: Cayratia trifolia, Phytochemical screening, Antioxidants, HPTLC P-046: CHEMICAL COMPOSITION AND ANTIOXIDANT ACTIVITY OF ESSENTIAL OIL FROM CURCUMA AMADA ROXB. Vishnupriya.M, Sasikumar J.M, Nishaa S, Teepica Priya Darsini D, Hephziabah Christabel P and Gopalakrishnan V.K Karpagam University, Coimbatore, Tamil Nadu This study was designed to examine the chemical composition and In vitro antioxidant activity of essential oil of Curcuma amada Roxb. The GC- MS analysis of the oil resulted in the identification of 12 compounds. -myrcene (63.85%) and -asarone (30.27%) were the two major components identified. The sample was subjected to screening for their possible antioxidant activity by using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical, ABTS radical, Ferric reducing antioxidant power and -Carotene bleaching assay. Results showed that the essential oil possessed a strong degree of antioxidant activity in terms of -Carotene bleaching capacity followed by ABTS radical, ferric reducing power and a moderate DPPH radical scavenging activity. This study concludes that the essential oil from Curcuma amada Roxb could serve as an important bioresource of antioxidants for using in food and pharmaceutical industry. Keywords: Curcuma amada, Essential oil, GC-MS analysis, Antioxidant activity P-047: EFFECT OF AQUEOUS EXTRACT OF HYBANTHUS ENNEASPERMUS IN GENTAMICIN INDUCED RENAL DAMAGE M. Manjunath Setty, K.K. Srinivasan and Annie Shirwaikar Manipal College of Pharmaceutical Sciences, Manipal University, Manipal Hybanthus enneaspermus (L.) F. Muell (Violaceae) known as Purusharathna in Sanskrit, is an important plant in the Indian system of medicine. It is a small suffrutescent perennial herb. Traditionally the plant is used as an aphrodisiac, tonic, diuretic, dysuria, urinary calculi, diarrhea, leucorrhoea and sterility. In spite of their manifold uses in kidney disorders the plant is not validated for nephrotoxicity studies. Hence the aqueous extract of the entire plant was studied for its nephroprotective activity in gentamicin-induced acute renal injury in albino rats. Wistar male rats divided randomly into 4 groups of 6 animals to study curative activity. Group 1 received s.c. dose of saline and group 2, 3 and 4 received s.c. dose of gentamicin 80 mg/kg from day 1 to day 8. From day 9 upto day 18, group 1 and 2 received 2% gum acacia (p.o) and group 3 and 4 received 250 and 500 mg/kg (b/w) of aqueous extract. From all the above groups blood was withdrawn and kidneys were removed on day 19. Various biochemical parameters such as blood urea, serum creatinine, TBARS, GSH, GST and SOD were estimated. Histopathological examinations of kidneys were carried out to determine the extent of tissue damage/healing. Aqueous extract of H. enneaspermus significantly decreased elevated blood urea and serum creatinine levels and also reverted the extent of kidney damage at both dose levels. Our studies showed that aqueous extract possesses marked nephroprotective activity and thus has a promising role in the treatment of gentamicin induced renal injury. Keywords: Hybanthus enneaspermus, nephrotoxicity, nephroprotective, gentamicin P-048: IMPROVED ABSORPTION AND BIOACTIVITY OF PLUMBAGIN FROM HYDROPHILIC LIPID BASED EXTRACT OF PLUMBAGO ZEYLANICA C. Bothiraja , Prajakta P. Joshi , Dama G.Y , Atmaram P. Pawar Department of Phytopharmaceutics, Sharadchandra Pawar College of Pharmacy, Otur, Pune The methods available for extraction of plumbagin from Plumbago zeylanica using non-edible organic solvents have various shortcomings. Alongside its poor aqueous solubility results in low bioavailability. The objective of this study was to obtain water soluble form of plumbagin single step technique using hydrophilic lipid Gelucire 44/14. It involved preparation of Gelucire extract (GPZ) and its evaluation by chromatographic and thermal analysis. The GPZ showed significantly higher extraction efficiency (3.24 ± 0.12% w/w) compared to ethanolic (EPZ) and aqueous (APZ) extraction, 2.48 ± 0.16% w/w and 0.07 ± 0.02% w/w, respectively. Further, in-vitro rat intestinal absorption and bioavailability of plumbagin from GPZ studied using non-everted rat intestinal sac model was compared with that of the APZ, EPZ and standard plumbagin. GPZ displayed significantly higher Q30min (cumulative percentage absorption of plumbagin in 30 min) and lower t40% (time required for 40% w/w drug absorption). The flux and apparent permeability coefficient in duodenum and ileum too was 2, 3 and 6 fold higher than EPZ, standard plumbagin and APZ, respectively. This was attributed to the micellar solubilization effect of Gelucire, consequently favoring partitioning of plumbagin through intestinal membrane. Besides, the use of GPZ did not cause histopathological changes in the treated rat intestine. The increase in solubility and bioavailability of GPZ was also apparent in the in-vivo anti-inflammatory study conducted in rats. Thus extraction using Gelucire can be proclaimed as an efficient, economic and solvent-free technique for extraction of plumbagin and can be utilized for various clinically important water insoluble phytoconstituents in order to improve their biopharmaceutical properties. Keywords: Plumbago zeylanica, Gelucire 44/14, Non-everted rat intestinal model P-049: EVALUATION OF A NOVEL HERBAL OIL EXTRACT FORMULATION Padmini Ravikumar, Rashmi Mallya and Lokesh Bhatt
SVKM's Bhanuben Nanavati College of Pharmacy Herbal oils also called oil extracts are traditional herbal remedies used to treat skin conditions, nourish and revitalize the skin. However these oils are not consumer friendly. In this study an attempt has been made to investigate the therapeutic properties of developed novel emulsion formulations. An oil extract containing Rubia cordifolia, Glycyrrhiza glabra, Symplocos racemosa, Citrus limoni was prepared . The mentioned plants have proven wound healing, antiseptic, skin soothing and anti-inflammatory properties. The oil extract in this study forms the oil phase for the formulation of the emulsion system. Creams and emulgels represent a promising carrier system for oily extracts due to their numerous advantages like aesthetic appeal, better efficacy, stability and appearance. The physiochemical parameters of the final formulation like appearance, pH, viscosity and spreadability were determined. Stability studies as per ICH guidelines are in process. The selected formulation has also been studied for skin irritation and wound healing activity in vivo by excision wound healing model on rats. The formulation of herbal oil into an emulsion based system proves to be safe, user friendly and suitable for skin conditions. Keywords: Herbal oil, emulsion system, wound healing P-050: PHYTOCHEMICAL INVESTIGATIONS AND PHARMACOLOGICAL EVALUATION OF ZANTHOXYLUM RHETSA ROXB. (BARK) FOR ANTI-DIABETIC ACTIVITY Vasudev pai, Rudraprabhu V Savadi, and Ananth Bhandarkar Manipal College of Pharmaceutical Sciences, Manipal University, Manipal Bark of Zanthoxylum rhetsa commonly known as "Mullilam" belongs to the Rutaceae family and is widely distributed in India. Zanthoxylum rhetsa is a medicinal plant, traditionally used in diabetes, antispasmodic, diuretic, anti-inflammatory and various ailments. The present study includes phytochemical and pharmacological evaluation of bark. Here the powdered bark was subjected to successive soxhlet extraction using different solvents of increasing polarity. The successive methanolic and chloroform extracts were subjected for in-vivo anti-diabetic evaluation. The methanolic extract was found more effective. So it was further subjected for preliminary phytochemical and chromatographic examination. Attempt was made to isolate the compounds using isocratic elution technique. The compounds isolated were characterized by UV, FTIR, H-NMR, 13C-NMR, MS and Characterization revealed that COMP-I may be a flavonoid and COMP-II may be alkaloidal moiety. Keywords: Zanthoxylum rhetsa, antidiabetic, dibetes, phytochemical investigation P-051: EVALUATION AND FORMULATION OF NATURAL HAIR COLORANTS Rashmi Mallya, Padmini Ravikumar and Nishad Kadiri SVKM's Bhanuben Nanavati College of Pharmacy Conventional methods of dyeing the hair involve the use of chemicals that result in unpleasant side effects which include irritation, breakage of hair, skin discoloration and cancer. Marketed hair colors containing plant material require processing prior to use which is inconvenient to the consumer. Also, such products have poor rinsability. Hence there is a need to formulate hair dyes containing crude drugs which are ready to use with good rinsability properties. In this study, an attempt has been made to develop formulations containing natural hair colorants like Lawsonia inermis, Emblica officinalis, Rubia cordifolia, Terminalia belerica and Juglans regia. The results indicate that except henna extract, aqueous extracts of individual plants used in this study do not impart color to human hair. Combination of extracts prepared by cold and/or hot extraction method also did not give encouraging results. A formulation containing a combination of the specified plant powders in 2% Carbopol 934 gel shows promising results and does not produce skin irritation in rats. Thus the formulated gel in addition to being consumer friendly is stable and safe for use. Keywords: Natural hair colorants, rinsability, gel P-052: GLOBAL REGULATORY POSITION OF HERBAL MEDICINE: A REVIEW R. H. Parmar*, D. B. Pandya, T. R. Desai, V. B. Savalia R K College of Pharmacy, Tramba, Rajkot, Gujarat Herbal medicine has become a topic of increasing global importance, with both medical and economic implications. The idea that just because traditional medicine products come from natural sources they are completely safe is dangerously false. This lead to need for development of uniform standards for the quality, safety and efficacy of these products. Standardization, stability and quality control for herbal medicines are feasible, but difficult to accomplish because of complex mixtures of constituents and the constituents responsible for the therapeutic effects are unknown sometimes. Further national recognition and regulation of herbal medicine vary considerably across countries. Herbal products are well established as phyto-medicines in some countries, whereas in others their therapeutic claims are not allowed and regarded as foods. Since the risk of using herbal medicine is a global concern, development of a global regulatory system is needed. This review addresses statistics regarding factors applied in regulatory systems for the classification of herbal medicinal products in different countries, which include: description in a Pharmacopoeia monograph, prescription status, and claim of a therapeutic effect, scheduled or regulated ingredients or substances, or periods of use. The article also discusses harmonizing efforts initiated worldwide in the regulatory guideline development for commercialization of herbal medicines. Keywords: Herbal medicine, Regulatory
P-053: A STUDY OF EFFECT OF EXTRACTS OF CUSCUTA REFLEXA ROXB. ON CYCLOPHOSPHAMIDE INDUCED ALOPECIA Satish Patel, Vikas Sharma, Nagendra S. Chauhan, V.K. Dixit Department of Pharmaceutical Sciences, Doctor Hari Singh Gour Vishwavidyalaya, Sagar (M.P.) Alopecia is a dermatological disorder with psychosocial implications on patients with hair loss. Hair loss is one of the most feared side effects of cancer chemotherapy. This preliminary study investigated the efficacy of the extracts of Cuscuta reflexa Roxb. in promoting hair growth in cyclophosphamide-induced hair loss. Shaved sites were created on the back skin of three groups each of 6 freshly weaned 4-week old rats. All groups were treated with 125 mg/kg cyclophosphamide (CYP) intra-peritoneally once to simulate changes seen in human chemotherapy-induced hair loss. Extracts was administered orally (group II, III) for 10 days after administration of CYP. Group I received no test substance. Histopathology and gross morphologic observations for hair re-growth at shaved sites revealed active follicular proliferation in extracts treated groups. Extracts of Cuscuta reflexa may, therefore, be capable of promoting follicular proliferation or preventing hair loss in cyclophosphamide-induced hair loss. Keywords: Cuscuta reflexa, Chemotherapy, Alopecia P-054: POTENTIAL ROLE OF STRYCHNOUS POTATORUM EXTRACT ON DIABETES INDUCED RATS AND ITS HISTOPATHOLOGICAL ANALYSIS B. Arun Jyothi and Anupalli Rojarani Department of Genetics and Biotechnology, Osmania University, Hyderabad, Andhra Pradesh Diabetes mellitus is the most common endocrine disorder that impairs glucose homeostasis resulting in severe diabetic complications due to perturbation in utilization of glucose. India has become the diabetic capital of the world with over 20 million diabetics and it will increase to 57 million by 2025. Traditionally, herbal medicines are well known for their hypoglycaemic activities. Strychonous potatorum L.f. is an important medicinal plant, belonging to Strychnaceae family used in Ayurveda, Unani, Siddha, and in folk medicine for treating several ailments. In India it is popularly used to purify water. Polysaccharide gum, alkaloids mainly diaboline, and four triterpenes viz., isomotiol, sitosterol, stigmasterol and compesterol were reported from seeds and leaves of S.potatorum. For the present study, animals were grouped into normal rat(control), diabetic (streptozotocin), diabetic but extract treated, only extract treated and diabetic but standard anti-diabetic drug tolbutamide treated group and were examined for the effect of ethanolic extract (250 and 500 mg/kg body weight) of Strychonous potatorum seeds and its histopathological study was carriedout. The extract improves the liver, kidney and pancreas function and reduce lesions associated with diabetic state in induced rats. The effect of oral administration of extract at a dose of 500 mg / kg body weight has more efficacy than the 250 mg/kg body weight. The results indicate the protective effect of extract on tissues and prove its potential as an antidiabetic agent. Keywords: Diabetes, Strychnous potatorum, histopathological, triterpenes, tolbutamide P-055: PHARMACOGNOSTIC EVALUATION AND ESTIMATION OF GALLIC ACID AND QUERCETIN IN CAREYA ARBOREA ROXB. LEAF BY HPTLC METHOD Prakash Chandra Gupta*1, Nisha Sharma1, Ch.V. Rao2 1University Institute of Pharmacy, C.S.J.M. University, Kanpur; 2NBRI, Lucknow Careya arborea Roxb. (Family: Lecthyidaceae) known as Kumbhi in Ayurvedic system of medicine, is an important medicinal plant used extensively for treating a variety of ailment in various system of indigenous medicine. In the present study, pharmacognostic profile and high performance thin layer chromatographic (HPTLC) method was developed for the estimation of gallic acid and quercetin in the leaf of Careya arborea. The pharmacognostic evaluation of Careya arborea leaf includes macroscopic and microscopic characters which will be helpful for the correct botanical identification of the drug. Macroscopically, the leaves are simple, broadly obovate in shape, acuminate apex with crenate, dentate margin and microscopically, the leaf showed the presence of median large size vascular bundle covered with fibrous bundle sheath and arrangement of xylem in cup shape. The hydroalcoholic extract of Careya arborea leaf was chromatographed on precoated silica gel 60F 254 plates with Toluene: Ethyl Acetate: Formic Acid (7:5:1 v/v/v) as mobile phase for both gallic acid and quercetin. Detection and quantification were performed densitometrically at 278 nm for gallic acid and quercetin. The standard Rf values of gallic acid and quercetin are 0.24±0.01 and 0.35±0.01 respectively. High performance thin layer chromatography showed the presence of gallic acid and quercetin (8.119 mg/g and 3.078 mg/g respectively) in the leaf of Careya arborea plant. Keywords: Careya arborea, gallic acid, quercetin, HPTLC P-056: EVALUATION OF GENETIC DIVERSITY BY RAPD FINGERPRINTING IN THE GAMMA IRRADIATED MUTANT PLANTS OF JATROPHA CURCAS L Vijay L. Ghori1, Shaik G Mastan2, PDVN sudheer2, H. Rahman2, Ch Ravi prakash2, Jitendra chikara2 1Shantilal Shah Pharmacy College, Bhavnagar University, Bhavnagar; 2CSMCRI, Bhavnagar J. curcas Linn. (Euphorbiaceae) is a genus comprising 70 species distributed in tropical and sub-tropical parts of the world. J. curcas is having high commercial and medicinal value viz. seeds, leaves and bark have been used in traditional medicine for a long time having the duel importance such as bio-energy and pharmaceutical. Studies conducted till date on J. curcas reveals limited genetic diversity in naturally available germplasm which has become one of the limiting factors for the application in different breeding programs for improvement of the
species. Thus we are trying to develop some genetically varied individuals by creating mutations. In the present study efforts were made to evaluate the diversity generated among the mutants obtained by giving predetermined dose of -radiations using Random amplified polymorphic DNA (RAPD) markers. The objective of the present study includes Screening and selection of 15 plants using RAPD primers, Generation of RAPD fingerprints using selected primers, Construction of binary matrix from the generated fingerprints, Estimation of the genetic similarity, genetic disparity, percentage of polymorphism and its analysis and Generation of dendrogram. Thus the present study aids in the identification of polymorphic markers obtained due to mutations and generation of physical maps and QTL (Quantitative Trait Analysis) and MAS (Marker Assisted Selection). Keywords: RAPD, Random amplified polymorphic DNA P-057: ANTI ARTHRITIC ACTIVITY OF ALCOHOLIC AND WATER EXTRACTS OF CAESALPINIA DECAPETALA STEM FREUND'S ADJUVANT INDUCED ARTHRITIS RATS Viren G. Patel*, Hardik. P. Patel, Kritika J. Patel S. K. Patel College of Pharmacetical Education & Research, Ganpat Universtiy, Kherva, Gujarat Objective: The aim of investigation was to evaluate the anti-arthritic activity of the water and alcoholic extracts of Caesalpinia decapetala (Leguminoseae (Fabaceae)) stem part. C. decapetala is a shrub bearing thorns and fine hairs on the inflorescence and red stem. It is known as Kirmich, Chilar in Gujarati. It is used in anthelmintic, antiperiodic, astringent, emmenagogue, febrifuge, laxative and purgative. Decoction of stem is usefull in the treatment of jaundice and rheumatism. Method: The anti-arthritic activity of C. decapetala was carried out by freund's adjuvant induced rats arthritis model after approval of Institutional Animal Ethical Committee. Water and Alcoholic extracts of C. decapetala 100 mg/kg and 200 mg/kg, 1 % Carboxymethyl cellulose was used as vehicle control, dexamethasone 1 mg/kg positive control given orally to rat. Anti-arthritic activity was evaluated by determining physical parameters (Inflammation, mobility test, Gait test, Joint stiffness, joint hyperalgesia), hematological parameters (red blood cells (RBC), white blood cells (WBC) and hemoglobin), and biochemical parameters (Erythrocyte Sedimentation rate (ESR) and Rheumatoid Arthritis Factor (RA factor). Results and Discussion: Results of anti arthritic activity of alcoholic and water extracts of C.decapetala were significant as compared to vehicle treated control groups. % inhibition of rat paw volume from 0, 7, 14, 21 and 28 days, gait score, mobility, joint hyperalgesia and joint stiffness from 7, 14, 21 and 28 days, RBC, WBC, hemoglobin, erythrocyte sedimentation rate (ESR) and rheumatoid arthritis (RA) factor at 28 day were very significant in both alcoholic and water extract 100 mg/kg and 200 mg/kg respectively as compare to vehicle treated control groups. Conclusion: C. decapetala contain glycoside, saponin, tannin, -sitosterols, stigma sterols and lupeol in their stem part raw materials. These constituents may be responsible for significant anti arthritic activity. Keywords: Anti-arthritic activity, Caesalpinia decapetala, Freund's adjuvant P-058: EVALUATION OF LIPID METABOLISM MODULATORY EFFECT OF A POLYHERBAL FORMULATION (ATH10) IN HYPERLIPIDAEMIC ANIMAL MODELS Jay Raja1, Kyada Ashish1, Chandani Kyada1, Devender Pal Sigh2, Purnima Ashok 1S.J.Thakkar Pharmacy College, Rajkot; 2QA Department, Ranbaxy Pharmaceuticals, Mohali The present investigation is aimed at evaluating lipid metabolism modulatory effect of a polyherbal formulation ATH10 in Wistar rat models. Individual herbs of ATH10 have been reported to possess antioxidant and antihyperlipidaemic activity. Effectiveness of ATH10 was measured in Triton WR-1339-induced (100 mg/kg) acute hyperlipidaemia and high fat diet-induced chronic hyperlipidaemia. Animals in test groups were treated with three different doses of ATH10 (100 mg, 200 mg and 400 mg/kg b.wt.). In triton WR-1339 induced hyperlipidaemic rats, blood samples were collected at 0 h, 6 h, 24 h and 48 h and plasma lipid profile was estimated. In chronic model, blood samples were collected on day 0 (basal value), day 15 (induction value) and day 30 (post treatment value) and lipid profile was measured. LDL and VLDL levels were calculated by using Friedwald's formula. and atherogenic index was calculated. At the end of study period, fecal cholesterol excretion and hepatic HMG-CoA reductase enzyme activity were also measured. In acute model, ATH10 at the dose of 400 mg/kg significantly lowered TC, TG, VLDL-C, LDL-C (p<0.01) with subsequent increase in high HDLC (p<0.01) at 6 h and 24 h. In chronic model, all doses significantly reduced TC and LDL-C levels with increase in HDL-C levels (P<0.001). ATH10 significantly decrease atherogenic index (P<0.01) with increase in HDL/TC ratio (P<0.01). Fecal cholesterol excretion was also significantly enhanced (P<0.01) in all the test doses of ATH10. Hepatic HMG-CoA reductase enzyme activity was found to be significant at 400mg/kg (p<0.05) (measured as an ratio of absorbance of HMG-CoA and mevalonate). Elevated levels of plasma LDL-C and its subsequent oxidative modification have been implicated as an early event in the initiation and progression of atheromatous plaque. Antioxidant property of the ATH10 could be beneficial. The above findings suggest that lipid metabolism modulatory effect of ATH10 could be due to interference in biosynthesis, metabolism and excretion of lipid. Keywords: LDL-C, Hyperlipidaemia, Atherogenic index, Polyherbal formulation P-059: COMPARATIVE DIURETIC ACTIVITY OF PERICARP & SEED EXTRACTS OF TRAPA NATANS LINN Madhuri H. Sadhwani, Brijal N. Asari, Ami S. Patel, Vaishali N.Shah APMC College of Pharmaceutical Education and Research, Himmatnagar
Fruit of Trapa natans L. var. bispinosa commonly known as Shingoda (Trapaceae) and is reported as antiinflammatory and diuretic activity in traditional literature. The present research investigated the diuretic activity with both pericarp and seed extract of fruits of Trapa natans. Preliminary phytochemical screening was done for the presence of various phytoconstituents. TLC densitometric method was developed using HPTLC for the quantification of this marker compound (Gallic acid) from the pericarp extract of Trapa natans. For diuretic activity test groups received aqueous extracts of pericarp and seed (200 mg/kg and 300 mg/kg bw). Phytochemical screening of seed extract of Trapa natans shows the presence of carbohydrate, Saponons, phytosterol, fixed oil, and fat, while pericarp extract of fruits of T.natans revealed the presence of tannins, Flavanoids and glycoside. Gallic acid content in pericarp extract was found to be 0.62%w/w using HPTLC fingerprinting. In acute oral toxicity study, it was observed that there was no mortality at any doses up to 2 gm/kg. The highest diuretic activity was presented by the aqueous pericarp extract. There is significant increase in Na+ and Cl- excretion in pericarp extract compared to control & seed. Results of this study showed more significant diuretic activity compare to seed extract may be to the presence of tannins and flavanoids. P-060: ANTIOXIDANT ACTIVITY OF ISOLATED COMPOUNDS FROM ARTABOTRYS HEXAPETALUS LEAVES BY DIFFERENT METHODS Paralkar Shrirang J., Tukra Rishbh R., Jain Nikita S.,Pal Subodh C., Aher A.N. NDMVP College of Pharmacy, Nashik Artabotrys hexapetalus is globally distributed in India, Sri Lanka, Java and South China. Two anthraquinones, isoquinoline alkaloids, apigenin, quercetin, lutein were isolated from the leaves of the plant.The antifertility, analgesic and anti-inflammatory activity, anthelmintic activity were performed from extract of the plant. The present study was performed to isolate phytoconstituents from leaf extracts of the plant. The total phenolic content and total tannins were 16.03% w/w and 11.79% w/w in the methanolic extracts of the leaves. Antioxidant activity was performed by DPPH, Nitric oxide free radical scavenging method, Hydrogen peroxide scavenging method and phenylhydrazine induced haemolysis of erythrocytes method. Antioxidant activity of isolated fractions was compared with the standard drug ascorbic acid. RP-1 and ES-1 found to be good scavenger of DPPH radical with 45.21% and 40.22% IC50 respectively. ES-1 shows good nitric oxide scavenging activity with 43.27% IC50. ES-1 shows good hydrogen peroxide scavenging activity with 52.53% IC50. ES-1 shows good and phenylhydrazine induced haemolysis of erythrocytes inhibition with 44.30% IC50. Keywords: Artabotrys, Antioxidant, Phenolics, DPPH P-061: PHYTOCHEMICAL AND PHARMACOLOGICAL EVALUATION OF CARTHAMUS TINCTORIUS LEAVES EXTRACT Pant Rahul, S.Vikram, Ch.V.Rao, Agrawal Kuldeep, Mishra Anupama Sri Rawatpura Intitute of Pharmacy, Datia (M.P.) Hepatoprotective activity of methanolic extract of leaves of Carthmus tinctorius L. (MECT) was investigated against hepatotoxicity produced by administering a combination of two anti-tubercular drugs Isoniazid and Rifampicin for 24 days by Oral route in rats. MECT were administered at two graded dose i.e. 200 and 300 mg/kg p.o. 45 min after to anti-tubercular challenge for 24 days. MECT was evident in the all doses as there was a significant decrease in AST, ALT, ALP, and Total Bilirubin levels. MECT significantly decreased the level of these biochemical parameter and elevated the level of GSH at all doses as compared to control. HPTLC analysis showed the presence of -sitosterol and Lupeol in MECT. Results showed that Carthamus tinctorius possess significant Hepatoprotective activity which might be due to flavonoids. Keywords: Carthamus tinctorius, Isoniazid , Rifampicin, Hepatoprotective activity P-062: ADAPTOGENIC ACTIVITY OF POLYGONATUM VERTICILLATUM (L.) ALL. RHIZOME EXTRACTS IN RAT Sandeep Kumar Singh, Vikas Sharma, Nagendra S. Chauhan, V. K. Dixit Hari Singh Gour Central University, Sagar, Madhya Pradesh Polygonatum verticillatum (L.) All. (Ruscaceae) rhizomes ethanolic extracts were screened for adaptogenic activity using swim endurance test and tail suspension model, the ethanolic extracts showed adaptogenic activity when administered orally at a dose of 50 mg/kg. The extracts increased the swim endurance time. Stress induced animals exhibited raised blood glucose level, serum cortisol level, cholesterol level and HDL as well as depletion in triglycerides. Keywords: Adaptogens, swimming endurance, tail suspension, blood glucose. P-063: CHRONIC TOXICITY STUDIES OF PLUMERIA ACUMINATA AND GALEGA PURPUREA IN EXPERIMENTAL ANIMALS Periyasamy Gomathi, Malaya Gupta, Upal Kanti Mazumder Vaagdevi College of Pharmacy, Kishanpura, Hanumkonda, Warangal, Andhra Pradesh The use of medicinal plants and preparations derived from them as dietary supplements, nutraceuticals, functional foods and herbal medicinal products has become more widely accepted in developing countries. Therefore, it is important to evaluate the adverse effects of these plants and their preparations. The objective of this study was to evaluate the safety of the methanol extracts Plumeria acuminata (MEPA) and Galega purpurea (MEGP) by single the long-term oral administration in mice. The study of chronic toxicity was determined by oral feeding male mice daily with the MEPA and MEGP at the dose of 300, 600 and 1,200 mg/kg body weight
continuously for 270 days. The results of chronic toxicity showed no abnormalities in the test groups as compared to the controls. Hematological and blood chemical values in treated groups were normal in comparison with the control group. Non-toxicity effect of MEPA and MEGP were present as no changes in body weight, internal organ weight, and general behaviors. Macroscopic or microscopic of internal organs or tissues in treated rats showed no changes. Therefore, the methanol extract of MEPA and MEGP given orally to male mice did not produce both acute and chronic toxicities. Keywords: Plumeria acuminata, Galega purpurea, chronic toxicity P-064: ISOLATION AND ESTIMATION OF PTERYGOSPERMIN IN MORINGA SPECIES AND MARKETED PREPARATION SEPTILIN BY HPTLC METHOD Tukra Rishabh J., Paralkar Shrirang J., Jain Bhushan B., Pal Subodh C., Aher A.N. NDMVP College of Pharmacy, Nashik Moringa oliefera is indigenous in the sub Himalayan tract from the Chenab to the Sarda also in the Outh forests. This plant is cultivated throughout India. Many scientists investigated different constituents from different parts of the plants. carotene, sterols, lecithin, different fatty acids, different vitamins has been isolated from seeds. Quercetin, Rhamnetin and Kaempoferol were isolated from flowers. Niazimin A, Niazimin B, Niazicin A and Niazicin B were isolated from leaves. 4-hydroxy mellein, - sitostenone, octacosanoic acid, sitosterol, hydroxyproline, leucodelphinidin glycoside has been reported from stem. The antibiotic principle Pterygospermin isolated from root. In this paper, the focus is given on isolation of Pterygospermin and flavonoids. The quantification of Pterygospermin in the plant part and also in the marketed preparation Septilin by HPTLC method. The amount of Pterygospermin found in the Moringa oliefera and Tablet extract was found to be 0.235% and 0.064% respectively. Keywords: Moringa, Pterygospermin, HPTLC P-065: PRELIMINARY PHYTOCHEMICAL SCREENING AND INVITRO ANTHELMINTIC ACTIVITY OF ARGEMONE MEXICANA ROOT Garad S.V., Poul B.N.,Usnale S.V. Marashtra college of pharmacy, Nilanga Dist. Latur (MS) India is the largest pool of medicinal plants with variety of pharmacological uses. It is present times need to investigate and confirm the uses with new techniques. Argemone mexicana roots are having anthelmintic activity. Presnt study reveals preliminary phytochemical constituents present in the root and invtro anthelmintic activity on Indian earthworm. In preliminary phytochemical investigation of root carbohydrates, flavonoids, alkaloids, tannins and steroids are found. In vitro anthelmintic activity is carried out on Indian earthworms using suitable anthelmintic reference standard. P-066: FORMULATION OF AN EDIBLE, BIODEGRADABLE, ENVIRONMENTAL FRIENDLY, SAFE AND EFFECTIVE ANTI-ULCER CHEWING GUM Vishnoupriya C, Chinta sruthi, Deepthi M, S. Indumathi, R. Jayapragash, Prakash Yoganandham, Gopal V Mother Theresa Post Graduate and Research Institute of Health Sciences, Puducherry Chewing gum today is gaining conservation as a vehicle or a delivery system to administer active principles that can improve health and nutrition. But its potential as an alternative drug delivery system has not yet been fully discovered and exploited. The drugs that are released from chewing gum and swallowed will be introduced in the gastro intestinal tract either dissolved or suspended in saliva and thus will be present in a readily available bioavailable form. Keeping in view the above light of information the present research work aims at formulating and evaluating an edible, biodegradable, environmental friendly, safe and effective herbal anti-ulcer chewing gum. Scientifically proven, popular herbal anti-ulcer drugs such as juice of Aloe barbadensis (Liliaceae), roots of Glycyrrhiza glabra (Leguminosae) and rhizomes of Curcuma longa (Zingiberaceae) were selected to formulate anti-ulcer chewing gum. It was thought to formulate a biodegradable chewing gum for this study. Hence, the fresh latex from the unripe fruit stalks of Manilkara zapota (Sapotaceae) were collected and used as a chewing gum base. The raw material was dried, size reduced and boiled in distilled water to get the aqueous extract. 500mg of aqueous extract of each drug were added to the latex with constant stirring till a uniform mass was formed. 1ml of glycerin and honey was finally added and allowed to cool at room temperature. The prepared gum was evaluated for its organoleptic, plasticity, stickiness, hardness and stability studies. The results obtained from the evaluation exhibit that the prepared chewing gum was found to be edible, tasty and stable with good patient compliance. Keywords: chewing gum P-067: APOPTOSIS STUDIES ON FLAVANOIDS ISOLATED FROM MEDICINAL PLANTS AGAINST HUMAN CANCER CELL LINE HepG2 Ramya Pydi, Benito Johnson and S Kavimani Mother Theresa Post Graduate and Research Institute of Health Sciences, Puducherry The flavanoids 5, 4-dihydroxyl 6, 8-dimethoxy flavones 7-o-rhamnoside and 5, 7, 4-trimethoxy flavones isolated from Indigofera aspalathoides and Bauhinia veriegata respectively were subjected to the apoptosis studies using human cancer cell line HepG2. Hoechst staining and fluorescent microscopy assay were employed to detect apoptosis. At a dose of 500µg/ml, a marked increase in the number of HepG2 cells with the typical
features of apoptosis consisting of cells with condensed and fragmented nuclei confirmed the apoptosis activity of isolated flavanoids. Keywords: Apoptosis, flavanoids and HepG2 P-068: HEPATOPROTECTIVE ACTIVITY OF ALCOHOLIC EXTRACT OF COLDENIA PROCUMBENS ON DGALACTOSAMINE INDUCED RATS R. Ganesan, Mathuram Venkatanarasimhan,G.Pramod Reddy, Sharad Pawar, K. Meenakshi Sundaramoorthi, Prema Gurumurthy Department of Biochemistry, Siddha Central Research Institute, Chennai, Tamilnadu The alcoholic extract of Coldenia procumbens in Tween 80 (Vehicle) was evaluated for hepatoprotective activity in Wister albino rats by inducing hepatic injury with D-galactosamine (200 mg/kg body weight intraperitoneally i.p.). D-galactosamine induced hepatic damage was manifested by a significant increase in the activities of marker enzymes. Biochemical data exhibited significant hepatoprotective activity of alcoholic extract of Coldenia procumbens at oral dose of 200 and 400 mg/kg against D-galactosamine. Silymarin was used as reference standard also exhibited significant hepatoprotective activity against D-galactosamine. The biochemical observations were supplemented with histopathological examination of rat liver sections. Keywords: Coldenia procumbens, Hepatoprotective activity, D-Galactosamine P-069: NEPHROPROTECTIVE EFFECT OF TRIBULUS TERRESTRIS ON GENTAMICIN INDUCED KIDNEY DAMAGE J. Mercy Jasmine1, V. Malathi1, D. Sharad Pawar2, R. Ganesan2, G. Pramod Reddy2* G. Veluchamy2 1Ethiraj College For Women, Chennai, T.N.; 2Siddha Central Research, TN Objectives: Tribulus terrestris is a perennial plant, found predominantly in India and Africa. This study is proposed to investigate the nephroprotective effect of Tribulus terrestris on gentamicin induced kidney damage in rats. Design: The kidney damage was induced by the i.m injection of Gentamicin at 80 mg/kg from 6th day onwards in Groups II, III, and IV. Group I and II were kept as normal (Saline) and nephrotoxic respectively. Group III and IV were treated with Tribulus terrestris at 300 mg/kg and 500 mg/kg of body weight in oral route respectively, for 10 days. The blood samples were collected and estimated for Creatinine, Blood urea nitrogen (BUN), Uric acid, Total protein, Albumin and Globulin. The kidneys were collected, weighed and processed for histopathology. Results: In the Group III (P <0.05) and Group IV (P<0.02) there was a significant reduction in the BUN, Creatinine, Uric acid, Total Protein, Albumin and Globulin levels as compared to that of Group I and II. (Table I & II). In the sections of the kidney obtained, there was a mild degree of damage in Group IV (+), followed by Group III (++) as compared to damage in Group II (+++).There was no damage in the sections obtained from Group I, which was normal control. Conclusion: From the results obtained it can be concluded that the alcoholic extract of Tribulus terrestris showed a significant decrease in the levels of Non-Protein Nitrogen compound levels and Protein levels probably due to its antioxidant property, thus it may be an effective nephroprotector. Keywords: Gentamicin, Kidney, Nephroprotective, Nephrotoxicity, Tribulus terrestris P-070: ETHNOMEDICINAL PLANT KNOWLEDGE AND PRACTICE OF THE JASHPUR DISTRICTS OF CHHATTISGARH, INDIA Anupam Kumar Tiwari, R Mehta, Sharad Nema, B M Prasad and K K Soni Research Scholar, Department of Rural Technology, Guru Ghasidas Vishwavidyalaya, Bilaspur (CG) An ethnomedicinal study was conducted to document the indigenous medicinal plant knowledge and use by traditional healers in Jashpur District of Chhattisgarh, India from December 2008 to November 2009. The information including the various data such as local names, ailments and disease treated, therapeutic effects, part of plants used and method of preparation were obtained from traditional healers through individual and face to face interviews. Also the fidelity levels of the species were determined. At the end of the study, it is found that 35 taxa of medicinal plants are used for used for various treatment by the traditional herbal healers. These medicinal plants belong to 26 families, which are therapeutically, used against different disease, such as cough, cold, dysentery, diarrhea, ulcer, diabetes, weakness, snake-bite and skin disorders are covered. Part of the plants used, mode of drug administration in different ailments are discussed. Keywords: Traditional healers, Ethno-medicine, Ailments P-071: STANDARDIZATION OF PANCHVALKAL CHURNA Patel Sweta R.*, Patel Nikunjana A. S.K.Patel College of Pharmaceutical Education & Research, Kherva-Mehasana Standardization of herbal formulations is essential in order to assess the quality, purity, safety and efficacy of drugs based on the amounts of their active principles. The present research work is an attempt to standardize "Panchvalkal Churna" an ayurvedic polyherbal formulation used in the treatment of vaginal infection. The churna describes in Aryabhishek and Bhavaprakash nighantu contains bark part of Ficus benghalensis (Nyagrodha), Ficus religiosa (Asvastha), Ficus racemosa (Udumbara), Albizia lebbeck (Sirisha), and Thespesia populnea (Paras Pipalo) in equal quantity. The Panchvalkal churna was prepared as per Aryabhishek. Raw material as well as in-house preparation and the marketed formulation have been standardized on the basis of organoleptic characters, physical characteristics and physico-chemical properties. The set parameters were
found to be sufficient to evaluate the churna and can be used as reference standards for the quality control/quality assurance purposes. Keywords: Panchvalkal churna, standardization, physicochemical parameters, quality P-072: IN VITRO ANTICOAGULANT ACTIVITY OF MANGROVE SPECIES AVICENNIA MARINA Soni Bhoomika P.*, Patel Rakesh K. S. K. Patel College of Pharmaceutical Education &Research, Ganpat University, Kherva,Gujarat Drugs from natural origin can help to get the new molecular lead as a hope towards the better efficacious and safe anticoagulant. The Present investigation was planned to evaluate invitro anticoagulant activity of methanolic extract, polysaccharide extract, Triterpenoid and Flavanoid extract from leaves and bark of Avicennia marina. Methanolic extract of Avicennia marina has demonstrated Presence of Carbohydrates, Triterpenoids, Steroids, Flavanoids and Tannins. Anticoagulant study by Prothrombin time test was performed on citrated plasma with different concentrations of extracts (100-1000µg/ml).The results shows that methanolic extracts of leaves prolongs the clotting time by 3.8,6.1,8.2 fold higher, respectively in 100,500,1000µg/ml than the control. Triterpenoid, Flavanoid and Polysaccharide extracts also Prolongs the clotting time and show anticoagulant activity. This study highlights that the anticoagulant activity of different extracts of avicennia marina affects intrinsic pathway of coagulation cascade by causing clotting factor(s) deficiency. Keywords: Avicennia marina, anticoagulant, Prothrombin time test, Flavanoids P-073: PHYTOCHEMISTRY AND COLUMN CHROMATOGRAPHY OF PHYSALIS MINIMA LEAVES Hardik Patel, N.M.Patel, Amit Patel, Jitendra Patel, Amish Patel Shri B. M. Shah College of Pharmaceutical Education & Research, Modasa, Gujarat Leaves and fruits of Physalis minima (Family Solanaceae; local name: Popti) has been in use traditionally for the treatment of diuretic, laxative, inflammation, enlargement of spleen, urinary diseases which are more commonly found on the bunds of the fields, waste lands, around the houses, on the road sides of India. In the present study, leaves of P.minima were subjected to phytochemical investigation. Phytochemical investigation of Leaves of Physalis minima showed presence of Steroids in Petroleum ether extract. Petroleum ether extract then subjected to Thin layer Chromatography and Column Chromatography. Three compounds were isolated by column chromatography in different fraction of Hexane: Ethyl acetate. These compounds are phytosterols which is confirmed by chemical test and TLC by antimony trichloride reagent. The Spectra of these compounds were obtained by UV spectroscopy, IR spectroscopy. These things suggest that Leaf extract contains Phytosterols. The same compound can be isolated and NMR spectroscopy and LC-MS analysis can be done to confirm the final structure and Anti inflammatory Animal models can be used to prove either they are responsible for Anti inflammatory activity or not. Keywords: Physalis minima, column chromatography, phytosterols. P-074: PHARMACOGNOSTIC AND PRELIMINARY PHYTOCHEMICAL STUDY OF COMMELINA BENGHALENSIS STEM Patel Roshani K.*, Patel Nikunjana A. S. K. Patel College of Pharmaceutical Education and Research, Kherva, Gujarat Plants are the great sources of medicines, especially in traditional system of medicine, which are useful in the treatment of various diseases. Commelia benghalensis is threatened medicinal herb belongs to the family Commelinaceae. The plant selected for the present study is used traditionally to treat fever, bone fractures, skin diseases, painful urinary discharges, and gonorrhea. Further, it has not yet been studied pharmacognostically. Hence, the present study is aimed to know the pharmacognostic parameters to determine the quality of the plant Commelina benghalensis.This study comprises the detailed macroscopic, microscopic and preliminary phytochemical investigations of the stem were studied. The microscopical evaluation of stem showed presence of multicellular uniceriate covering trichomes which were curved at apex. Paracytic stomata along with acicular calcium oxalates in epidermal cell and vascular bundle were also observed. Total ash value, acid insoluble ash value and water soluble ash value were found 25 %, 8.5 % and 13% respectively. Water extractive value was found to be more comparative to petroleum ether and ethanol. The preliminary phytochemical study reveals the presence of various phenolic components like tannins, phytosterols and flavanoids. Data's evolved in this investigation could be used in laying down pharmacopoeial standards for the drug studied, as standardization of herbal medicines is absolutely essential and is need of the hour. Keywords: Commelia benghalensis, Pharmacognostic, Phytochemical, microscopy P-075: COMPARATIVE EVALUATION OF VARIOUS FRACTIONS ISOLATED FROM FENUGREEK FOR INFLAMMATORY DISORDERS Kansara Nirali Y.*, Patel Rakesh K. S. K. Patel College of Pharmaceutical Education and Research, Ganpat University Fenugreek (Trigonella foenum graecum L. seed) is a food with traditional use in inflammation. Beneficial effects have been demonstrated in inflammatory animals by Carageenan induced rat paw oedema model. Pharmacognostic study was done by Powder flow properties and by physicochemical parameters. Phytochemical investigation was also done which shows presence of phytosterols, alkaloids, saponins and flavonoids. Anti inflammatory effects of a lipid extract, crude methanolic extract, further their sub fractions like
n-butanol fraction and steroidal aglycone of saponin glycoside and alkaloidal fraction from fenugreek seed powder were investigated In vivo using Carageenan induced rat paw oedema model. Characterisation of each fractions were done by chemical test and thin layer chromatography. All fractions show better anti inflammatory activity. Keywords: Trigonella foenum graecum, Inflammation, Carageenan, Paw oedema. P-076: QUALITY CONTROL PARAMETER AND LAXATIVE ACTIVITY OF SWADIST VIRECHAN CHURNA- A POLYHERBAL FORMULATION Sejal P. Patel, N.M. Patel, A. A. Patel, Shraddha R. Patel Shri B. M. Shah College of Pharmaceutical Education & Research, Modasa, Gujarat Standardization is an essential measurement for ensuring the quality control of the herbal drugs. Swadist Virechan churna consist of Cassia angustifolia, Foeniculum vulgare, Glycyrrhiza glabra, Sarkara and Shuddh Gandhak and used in constipation as a laxative. In present study the quality control of Swadist Virechan Churna is carried out by evaluting various parameters like powder fineness, moisture content, angle of repose, bulk density, tapped density, carr's index, hausner's ratio, pH, heavy metal analysis, microbial analysis, evalution of pharmacological activity and parameters for raw material includes organoleptic, physicochemical, phytochemical. Results indicate that Swadist Virechan churna has passed through all organoleptic and physicochemical parameters. Moisture content and pH of all ingredients and finished formulation are within limit so there is less chances of microbial growth and gastric irritation. Heavy metal analysis shows that all heavy metals are within limit. Total ash and water soluble extractive for all ingredients is within limit therefore it can be said free from impurities. Laxative activity of Swadist Virechan Churna on Wistar albino rats had showed significant fecal output after 8 hours as compared to control group. So, it is concluded that Swadist Virechan churna may be formulated as a laxative. Keywords: Standardization , laxative, Swadist Virechan Churna P-077: DEVEPLOPMENT OF QUALITY ASSESSMENT PARAMETERS FOR ANDROGRAPHIS PANICULATA Krupa Kalaria, Kruti Desai, Vidita Bhargava, Shrina Kalaria, Kishor Dholwani, Ajay Saluja Department of Pharmacognosy, A.R.C.P., Vallabh Vidyanagar Andrographis paniculata (Acanthaceae) is commonly known as "Kalmegh". It has good medicinal values in traditional system of medicine as hepatoprotective, febrifuge, bitter tonic, blood purifier, in bacillary dysentery, dyspepsia, for loss of appetite, anthelmintic, anti spasmodic, in diabetes, piles, bronchitis, debility, influenza, as an astringent, has anodyne property. So it became worthwhile to develop the quality assessment parameters of the plant. It was decided to evaluate the quality control parameters of Andrographis paniculata. Evaluation of the quality control parameters of Andrographis paniculata like morphological and microscopical studies and powder analysis were also done in which extractive value, ash value, moisture content were determined . Quantification of marker compounds andrographolide and gallic acid was done by HPTLC and HPLC. Values of proximate analysis, morphological and microscopical parameters are comparable with that of literature and parameters were assessed to standardize Andrographis paniculata. Validation of method was also carried out in terms of accuracy, precision, linearity, specificity, etc. P-078: SCREENING FOR ANTIMICROBIAL ACTIVITY AND PHYTOCHEMICAL CONSTITUENTS OF IXORA ALBA LINN. M.B. Talpade1, C.S. Sawant2, A.M. Bhagwat2 1Department of Zoology, Mithibai College, Mumbai; 2Shri C.B. Patel Research Centre, Mumbai Pathogenic bacteria contribute too many globally important diseases by virtue of their increasing resistance to the effects of antibiotics, in both community-acquired and nosocomial infections. Plants contain a fascinating array of natural products which have been traditionally used for treating many infections. Ixora species is one such plant which has ethnobotanical references indicating its use as a medicinal plant but has not been studied extensively. Antimicrobial activity and phytochemical constituents of methanolic extract of Ixora alba (bark) was investigated. Preliminary phytochemical screening of the crude methanolic extract revealed the presence of tannins, saponins, flavonoids, cardiac glycosides, bitter and pungent constituents, alkaloids, coumarins and essential oils. The crude extract displayed broad spectrum antimicrobial activity against clinically important microbial strains at the tested concentration (50 mg ml-1) showing excellent antibacterial activity against P. aeruginosa, H. parahaemolyticus and S. typhi. It also exhibited excellent antifungal potential against C. albicans and C. neoformans. The minimum inhibitory concentration (MIC) was determined using agar cup diffusion method. Based on the observations Ixora alba L. appears to be a valuable source for antimicrobial principles. Keywords: Ixora alba, antibacterial and antifungal activity, phytochemicals P-079: DEVEPLOPMENT OF QUALITY ASSESSMENT PARAMETERS FOR GYMNEMA SYLVESTRE Kruti Desai, Vidita Bhargava, Krupa Kalaria, Anisha Prajapti, Kishor Dholwani, Ajay Saluja Department of Pharmacognosy, A.R.C.P., Vallabh Vidyanagar Gymnema sylvestre belonging to family Asclepiadaceae, comonly known as "madhunashini" is used as remedy for diabetes, which is one of the major ingredient of anti-diabetic herbal formulations. It is also used as an astringent, stomachic, tonic, refrigerant, laxative, diuretic and cough. So it became worthwhile to develop the quality assessment parameters of the plant. For this morphological and microscopical studies were carried out by using fresh leaves of the plant. Powder analysis was also done in which extractive value, ash value, moisture
content were determined and also successive solvent extraction was carried out using Soxhlet's Extractor using different solvents as per the elutropic series. Thin Layer Chromatography studies of the extract were carried out, two phytoconstituents decayl gymnemic acid and gallic acid were quantified using HPTLC and HPLC methods. Validation of method was also carried out in terms of accuracy, precision, linearity, specificity, etc. and quality control parameters for standardization of Gymnema sylvestre leaf were established. P-080: SCREENING OF SELECTED HERBAL PLANTS FOR ANTI ACNE PROPERTIES KARADI RV, PANDEY C, GAVIRAJ EN, MANJUNATH KP, AKKI KS KLEU's College of Pharmacy, Hubli The objective of this study was to evaluate the in-vitro and in-vivo antiacne effects of ethanolic and aqueous extract of rhizomes of selected curcuma species (Curcuma aromatica, Curcuma amada) against Propionibacterium acnes. Minimum inhibitory concentration to inhibit the growth of P. acne of ethanolic extract of Curcuma spp. were found to be 125 g/ml and aqueous extract of Curcuma spp. were found to inhibit the growth of P. acne at concentration 250 g/ml. 140 µg of heat killed P. acnes injected in the ears of rats by subcutaneous route. Ear thickness was measured as an index of inflammatory strength using vernier caliper upto 35 days but there was no significant change after 10th day. Keywords: Propionibacterium acnes, Curcuma aromatica, Curcuma amada P-081: ANTICANCER ACTIVITY OF PENTATROPHIS MICROPHYLLA Modh Rashmi S.*, Patel Hardik P. S.K.Patel College of Pharmaceutical Education And Research, Kherva, Mehsana , Gujarat The ethnobotany and ubiquitous plants provide a rich resource for Natural drug research and development. In recent years, the use of traditional medicine information on plant research received considerable interest in Cancer Research. The Present Investigation was aimed for anticancer activity of Hydroalcoholic extracts of leaves, stem and roots of Pentatrophis microphylla. Hydroalcoholic extract of Pentatrophis microphylla has demonstrated presence of Phytosterols, Flavanoids and tannin. Effects of these compounds on the anticancer to both human breast cancer cell (MCF-7) and human prostate cancer cell (DU-145) were determined by the MTT methods. The Hydroalcoholic extracts of Pentatrophis Microphylla are showing potent Anticancer activity against MCF7 and DU-145 cell line. Keywords: Pentatrophis microphylla, Anticancer, Cell line P-082: STANDARDIZATION & ANTI ULCER ACTIVITY OF PEPGARD SYRUP-POLYHERB FORMULATION Shraddha R. Patel, N.M. Patel, A. A. Patel, Sejal P. Patel Shri B. M. Shah College of Pharmaceutical Education & Research, Modasa, Gujarat Evaluation is necessary to ensure quality and purity of the herbal product. Pepgard Syrup consists of nine ingredients eg. aqueous extract of De Glycyrrhizinated Glycerrhiza glabra Linn. (D.G.L), Asparagus racemosus Willd., Centella asiatica Linn., Embellica officinale Gaertn., Ipomoea turpethum Linn., Syzgium aromaticum Linn., Fumaria officinalis Linn., cold macerated extract of fresh herb Coriandrum sativum Linn. and powder of Sajjkhar (Astoneman indicum). It is used in peptic & duodenal ulcer, hyperacidity, heartburn, flatulence, and refluxoesophagitis. For evaluation of syrup containing single herb various parameters were tested. Results point out that raw material of `Pepgard syrup' has passed through all Organoleptic, Physicochemical, Physical and Phytochemical parameters. All three batches of `Pepgard Syrup' were uniform description, pH, viscosity, specific gravity, surface tension, total sugar, and total solid content etc. Heavy metal and microbial contamination was below the detection limit. Ulcer Index and histopathology study revealed that Pepgard Syrup had shown significant anti ulcer activity. Keywords: Anti Ulcer, Standardization, Pepgard P-083: ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY OF MYRICA NAGI BARK Kaushik B. Kanada1, Rakesh K. Patel2, M. M. Patel1 1Kalol Institute of Pharmacy, Kalol; 2SKPCPER, Ganpat University, Kherva, Gujarat Objective: The objective of present study was to evaluate antimicrobial and antioxidant activity of Myrica nagi Linn. Bark. Methods: The mixtures of different extracts of Myrica nagi bark were prepared using the SSE for the present study. Mixture A was the mixture of non-polar solvents extracts and Mixture B was mixture of polar solvents extracts. Both Mixtures were used in doses of 50 mcl for antimicrobial activity against Gram positive microorganisms like B. Subtilis, S. Aureus and Gram negative microorganism like E. Coli, P. Aeruginosa. Both mixtures were used in concentration of 200-1000 mcg/ml in methanol for in-vitro antioxidant activity by using DPPH and FRPA assay. Results: The successive solvent extracts showed significant presence of triterpenoids saponin glycoside, tannin and carbohydrate. The extract B showed a significant effect in inhibiting DPPH, reaching up to 86..41 % at concentration 800 mcg/ml and its IC50 was 324.67 mcg/ml while Extract A reaching up to 41.49 % at concentration 1000 mcg/ml and its IC50 was 1251.02 mcg/ml. This drastic change is due to the high content of phenolic compound present in the extract B as compared to the extract A. The Extract B showed a significant effect in reduction of ferric ion, with 87.39 % reduction at concentration 1000 mcg/ml and its EC50 was 313.4 mcg/ml while extract A with 65.37 % at concentration 1000 mcg/ml and its EC50 was 747.6 mcg/ml. This difference was due to the high content of phenolic compound present in the extract B. The Extract A was highly effective against E. coli and Staphylococcus aureus with all the concentration in dose dependant manner. Also Extract A was found to be effective against B. subtilis and P. aeruginosa in high concentration. The
Extract B showed significant antimicrobial activity against all four microorganisms- S. aureus, B. subtilis, P. aeruginosa and E.coli. Conclusion: The result of present study suggests that Myrica nagi bark possesses anti microbial and antioxidant activity. The work explored the pharmacological evaluation of Myrica nagi bark for various diseases like arthritis; hepatitis etc that provides a framework that may be useful in studies of other plant drugs. Keywords: Myrica nagi, anti oxidant, anti microbial P-084: ANTIULCER POTENTIAL OF STEM BARK OF BAUHINIA PURPUREA LINN. Vadnere GP*, Sathe BS, Teli NB, Md. Rageeb Md. Usman, Singhai AK Smt. S. S. Patil College of Pharmacy, Dept. of Pharmacognosy, Chopda (MS) OBJECTIVE: Highly valued drug in Indigenous System of Medicine for treatment of various ailments. Thus screening the possible potential of petroleum ether and methanol extracts of stem bark in treatment of acetic acid induced ulcerative colitis in mice. METHODS: Petroleum ether and methanol extracts of B. purpurea stem bark (100 mg/kg) were tested for its usefulness in UC by the method of acetic acid induced UC in mice. RESULTS: Methanol extract (100 mg/kg) reduced the level of MPO in blood to 278 ± 1.48 U/mL and 291 ± 1.32 U/mg in tissue. As well, it reduced the level of MDA in blood to 7.40 ± 0.12 nmol/mL and 6.14 ± 0.35 U/mg in tissue. Significance of results obtained was comparable with the standard drug, prednisolone (5 mg/kg). These protective and preventive effects of methanol extract were observed by morphological and histopathological studies. Petroleum ether extract lack that significance of effectiveness. CONCLUSION: The effect of crude extracts of stem bark of B. purpurea thus studied independent of other systems, thus relating direct mechanism of action to affirm the antiulcer effect and results as obtained thus justify the effectiveness in ulcerative colitis as compared to standard drug prednisolone in mice by methanol extract. Keywords: Ulcerative colitis, myeloperoxidase, malondialdehyde activity, histopathology P-085: STANDARDIZATION OF NAVAYASA LAUHA CHURNA Solanki Hiral D.*, Patel Rakesh K. S.K.Patel College of Pharmaceutical Education & Research, Kherva, Gujarat The standardization and quality control parameters of Ayurvedic formulations are not well defined. The efforts have been initiated to develop certain methods and parameters for standardization and quality control. Ayurvedic formulation-Navayasa lauha churna is a combination of equal parts of Triphala, Trikatu, the tubercles of the nut grass (nager musta), Embelia ribes (vidanga), and the roots of Plumbago zeylanica (chitraka) powder and nine parts of pulverized iron (lauha bhasma). In house preparations of churna were prepared as per Ayurvedic formulary. Raw materials, In house preparation and marketed churna have been standardized on the basis of organoleptic characters, physical characteristics and physico-chemical properties. Total tannins, total phenolics, total alkaloids, total flavonoids and total iron content were determined in the churna formulation. TLC fingerprinting of the chruna formulations were developed and compared with the ingredient drugs. The set parameters were found to be sufficient to evaluate the churna and can be used as reference standards for the quality control/quality assurance purposes. Keywords: Navayasa lauha churna, Standardization, Physicochemical parameters P-086: SCREENING OF ANTIDIABETIC ACTIVITY OF DESMOTRICHUM FIMBRIATUM BLUME Md. Rageeb Md. Usman*, Vadnere GP, Jagtap VJ, Somani PR, Singhai AK Smt. S. S. Patil College of Pharmacy, Dept. of Pharmacognosy, Chopda, (MS) OBJECTIVE: The present investigation aims to examine the hypoglycaemic and antidiabetic activity of whole D. fimbriatum in normoglycaemic and alloxaninduced diabetic rats. plant of METHODS: Methanol fraction of bioactive ethanol extract of whole plant of D. fimbriatum (DFMF) was administered (50, 100 and 200 mg/kg, p.o.) in normal fasted, glucose fed (1.5 g/kg, p.o.) and alloxan (120 mg/kg, s.c.)- induced diabetic rats (n=5). The blood glucose levels were estimated using One Touch glucometer (Johnson and Johnson). RESULTS: Oral pre-treatment with DFMF in normal fasted rats DFMF (50, 100 and 200 mg/kg, p.o.) dose dependently lowered (p< 0.05) the blood glucose as compared to control group. DFMF produced hypoglycaemic effect at 4 h after its administration. Glibenclamide produced significant (p< 0.05) decrease in blood glucose level. Its onset of action was at 4 h and peak effect showed at 6 h after drug administration. In glucose induced hyperglycaemic rat DFMF significantly (p< 0.05) prevented the rise in blood glucose after ½ h as compared to control glucose fed rats. Glibenclamide pre-treatment in glucose fed rats; significantly (p< 0.05) prevented the rise in blood glucose level after ½ h. In alloxan- induced diabetic rats DFMF (200 mg/kg, p.o.) significantly (p< 0.05) lowered the blood glucose after two weeks treatment. In these preliminary studies DFMF produced significant effect at the dose of 200 mg/kg, therefore, protective effect of methanol fraction of ethanol extract of whole plant of Desmotrichum fimbriatum Blume on alloxan induced diabetic rats could be attributed to regenerative properties of the fraction. CONCLUSION: The active polar constituents present in DFMF may recover the disorders in carbohydrate metabolism noted in diabetic state by stimulating existing cells or by increasing the rate of cell regeneration or by modulating intracellular glucose utilization. The actual mechanism is not clear and instigates for further investigations. Keywords: Desmotrichum fimbriatum, hypoglycaemic, antidiabetic
P-087: ANTIASTHMATIC ACTIVITY OF BARK OF MYRICA NAGI THUNB. Rana Rinu K.*, Patel Rakesh K. S.K.Patel College of Pharmaceutical Education & Research, Kherva, Gujarat Myrica nagi Thunb. (Myricaceae) is traditionally known as "Kaiphal" and reported to have various pharmacological activities such as antidibetic, antioxidant and analgesic. It is traditionally used in asthma as described in Nadkarni et al. In the present study, antiasthmatic activity of non polar and polar fractions of bark of Myrica nagi was evaluated using various experimental models i.e. Acetylcholine induced bronchospasm in Guinea pigs, Acetylcholine induced contraction on isolated Guinea pigs tracheal chain preparation, Compound 48/80 induced mast cell degranulation using rat and Trypsin and Egg albumin induced bronchospasm in conscious rats. The polar fractions have shown the presence of tannins and flavonoids, whereas non polar fractions have shown the presence of triterpenoids, phytosterols and saponins. The results of the study suggest that the plant possesses antiasthmatic activity due to its spasmolytic, mast cell stabilizing and bronchodilating properties confirming the traditional claims. Keywords: Myrica nagi, Antiasthmatic activity, Acetylcholine, Mast cell degranulation P-088: PHYTOCHEMICAL INVESTIGATION AND IN VITRO BIOLOGICAL SCREENING OF MALLOTUS PHILIPPINENSIS Patel Jalak P.*, Patel Rakesh K. S.K.Patel College of Pharmaceutical Education & Research, Kherva, Gujarat Herbal drugs have gained momentum and the demand for herbal raw drugs and other products is increasing many folds. Mallotus philippinensis (Family: Euphorbiaceae) is a popular anthelmintic plant possessing various pharmacological activities. The present work is an attempt to isolate different phloroglucinol derivatives from the powder of Mallotus philippinensis and to evaluate its In vitro antioxidant activity by DPPH method and cytotoxicity potential by MTT assay. The phytochemical investigation of M. philippinensis revealed the presense of large amount of polyphenols especially phloroglucinol derivatives. In the present investigation four compounds (Rottlerin, Isorottlerin, Compound- I and Compound- II) were isolated from the dried powder of Mallotus philippinensis. Rottlerin and Isorottlerin were isolated from M. philippinensis powder by the method described by Khorana et al. Compound- I and Compound- II were isolated from the petroleum ether and ethyl acetate soluble fraction of MeOH extract of M. philippinensis respectively. All the compounds were characterized by Melting point, TLC and IR spectroscopy. All the four compounds were evaluated for cytotoxicity activity by using MCF - 7 cell line and antioxidants activity by DPPH method. Keywords: Mallotus philippensis, Phloroglucinol derivatives, Anti oxidant P-089: EFFECT OF SHODHAN PROCESS ON STRYCHNINE CONTENT OF NUX VOMICA SEED Aarohi A. Prajapati, Kaushik B. Kanada, M. M. Patel Kalol Institute of Pharmacy, Kalol, Gujarat Introduction: Ayurvedic texts suggest the use of any toxic drug after suitable processing or detoxification, to reduce or modify poisonous effects of the drug and render it suitable for use in formulations for therapeutic use. Being a poisonous drug the nux-vomica has also been used after Shodhan (detoxification), for therapeutic purposes. Strychnos nux-vomica (Linn.), family Loganiaceae has been a very promising drug for certain diseases and mostly used as bitter stomachic, CNS stimulant and aphrodisiac. Objective: In the present study, an attempt was made for the phytochemical evaluation of Strychnos nux-vomica seed, before and after shodhan process. Material and Methods: The various shodhan processes were used as follow 1. Steaming of Nux-vomica seed with water, 2. Fried in cow ghee, 3. Cooked with cow milk, 4. Soaked in cow's urine for 7 days, then cooked in cow's milk and fried in cow ghee, 5. Purified powder of nux vomica obtained from the market. Macroscopic and microscopic evaluation, phytochemical and physicochemical evaluation and the TLC of extracts were performed. The assay of strychnine was performed by B.P. II 1958 method. Each process was conducted in triplicate. Result: It was found from the result that all the powder complied with standard value of physico-chemical parameters and Rf values of spots appears on TLC plate was also comparable with that of standard value of strychnine. The crude seeds without shodhan show 4.36 % of strychnine. The seed powder after shodhan process contains 1.841 %, 1.910 %, 2.046 %, 2 % and 2.12 % strychnine in process used respectively mentioned above. The analysis revealed a significant variation in strychnine content which might be due to different shodhan processes used. Keywords: Nux-vomica, shodhan process, strychnine P-090: EVALUATION OF PHYTOCHEMICAL AND PHYSICOCHEMICAL PARAMETERS OF ABHA CAPSULE Dipti M. Pokal, N.M. Patel, A. A. Patel, Rachana V. Patel Shri B. M. Shah College of Pharmaceutical Education & Research, Modasa, Gujarat The most important challenges faced by herbal formulations arise because of their lack of complete evaluation. Evaluation is necessary to ensure quality and purity of the herbal product. Abha capsule consists of four ingredients viz., extract of Cissus quadrangularis Linn, and powder mixture of Abha guggul, Kishore guggul, and Lakshdi guggul. It is used in Fracture healing, osteoarthritis, painful arthritic condition, relieves pain and inflammation. For evaluation of capsule containing single herb various parameters were tested. Parameters for raw material Physicochemical and phytochemical parameters. Parameters for finished product (capsule) include uniformity of weight, pH, moisture content, disintegration time and dissolution study. `Results point out
that raw material of `Abha capsule' has passed through all Physicochemical and Phytochemical parameters. All three batches of `Abha capsule' were uniform in colour, size, weight, dissolution and disintegration time, moisture content, angle of repose, bulk and tap density. Keywords: Physicochemical, Phytochemical, Abha capsule P-091: PHYTOCHEMICAL INVESTIGATION & ANTIMICROBIAL ACTIVITY OF AJUGA BRACTEOSA WALL Rachana V. Patel, N.M. Patel, A. A. Patel, Dipti M. Pokal Shri B. M. Shah College of Pharmaceutical Education & Research, Modasa, Gujarat Ajuga bracteosa Wall. ex Benth. herb belongs to family Labiatae; commonly known as Neelkanthi; is distributed in subtropical and temperate regions from Kashmir to Bhutan, Pakistan, Afghanistan, China and Malaysia. Traditionaly the herb having various biological activity such as anti-microbial, antitumor, anti-inflammatory, hepatoprotective, immunomodulatory, cytotoxic, antisecticidal and insect-antifeedant properties. Extraction of A. bracteosa Wall. ex Benth. herb in different solvents like petroleum ether, benzene, chloroform, acetone, ethanol and water. All the extracts were tested to know the different constituents present in them by the standard procedures. Total phenolic, flavonoid content in the ethanol and aqueous extracts and antimicrobial activity carried out. Results indicated that Ajuga bracteosa Wall. ex Benth. herb having significant antimicrobail activiy due to presence of different phytoconstituents. Keywords: Ajuga bracteosa, Antimicrobial, Phytochemical P-092: ESTIMATION OF TOTAL TANNIN CONTENT FROM RAW MATERIAL AND FORMULATIONS Dipali G. Makwana, Kaushik B. Kanada, M. M. Patel Kalol Institute of Pharmacy, Kalol Introduction: The common sources of tannins are Amla (Emblica officinalis, Family: Euphorbiaceae), Baheda (Terminalia belerica, Family: Combretaceae), Harde (Terminalia chebula, Family: Combretaceae) which are wellknown drugs used in the Ayurvedic medicine. Tannins are complex moieties produced by majority of plants as protective substances having wide pharmacological activities. They have been used since past as tanning agents and reported for astringent, anti-inflammatory, anti-diarrheal, antioxidant and antimicrobial activities. Objective: The various formulations containing Amla, Baheda and Harde are widely available in market, which shows wide variation in tannin content and quality. Therefore in the present study an attempt has been done to determine the tannin content of different five formulations. Material & Methods: The five formulations Amla powder (A), Baheda (B), Harde (C), Triphala powder (D) and Arandbhrust Haritaki (E) was taken. Macroscopic and microscopic evaluation, phytochemical and physicochemical evaluation and the TLC of extracts were performed. They were evaluated for other standardization parameters of powder such as powder fineness, loss on drying, water extractive value etc. In the present work the estimation of total tannins in different formulations is done by simple redox titration. Result: It was found that all the formulations complied with standard value of physico-chemical parameters and Rf values of spots appears on TLC plate was also comparable with that of standard value of tannins. The formulations contain 28% w/w, 32.84 % w/w, 36.55% w/w, 29.90% w/w and 33.256% w/w of tannin in formulation A, B, C, D & E respectively. The analysis revealed a significant variation in tannin content which might be due to different source of crude material and manufacturer. Keywords: Tannin, Amla, Baheda, Harde P-093: ANALGESIC ACTIVITY OF ESSENTIAL OIL EXTRACTED FROM COLEUS AROMATICUS LINN. LEAVES Agrawal A., Dehariya B., Sharma H.K., Verma C.J. Patel College of Pharmacy, Bhopal Coleus aromaticus (Lamiaceae) essential oil useful in bronchitis, asthma, cold, chronic cough, epilepsy, diarrhea. Coleus aromaticus is commonly considered as a medicinal plant, useful for relieving headache, colic, flatulence, rheumatism, otalgia (ear aches). The aim of the present study was to evaluate the analgesic activity of an essential oil extracted from coleus aromaticus leaves in mice. Essential oil was extracted by subjecting weighed amount of leaf to hydrodistillation by using Clevenger's apparatus. Finally the oil was collected. The collected oil was used for analgesic effect. Analgesic activity of essential oils at different dose levels was studied on 20-30gm Swiss albino mice. The reaction time at 15, 30, 60, 90, 120 minutes were measured. The pain sensitivity was assessed by the acetic acid induced writhing models in mice. Thirty mice (20-30 gm) were randomly divided into 6 groups. Group I was given distilled water 1 mL as negative control, group II Paracetamol 65 mg/kg BW as positive control, and group III-VI received an Essential oil of coleus aromaticus leaves in 4 doses, i.e. 50 mg/kgBW, 100mg/ kgBW, 250mg/kgBW, and 400mg/kgBW, respectively. All interventions were administered as a single dose by oral route on a given day. Acetic acid 0.6% (w/v) was used as a pain inductor. Analgesic activity was measured by counting the percentage of writhing movements as a measure of the analgesic effect produced by each intervention. Data were analyzed with one-way ANOVAs to compare analgesic activity between treatment groups. In the acetic acid-induced writhing test, the essential oil of coleus aromaticus leaves at the 200 and 400 mg/kg dose level showed 69.9 % and 72.5 % inhibition of writhing, respectively. These experimental results suggest that essential oil (200 and 400 mg/kg, p.o) produced analgesic effect in mice and may be a candidate for the development of pharmacological agents used in the pain management. Keywords: Coleus aromaticus, Analgesic activity, acetic acid induced writhing model P-094: EVALUATION AND STANDARDIZATION OF VARIOUS MARKETED FORMULATION OF RAUWOLFIA
Niketa N. Chhatraya, Kaushik B. Kanada, M. M. Patel Kalol Institute of Pharmacy, Kalol Introduction: The Sarpagandha (Roots of Rauwolfia serpentina, Family: Apocynaceae) is commonly used medicinally in the Modern and Western Medical system. The therapeutic value is mainly due to indole alkaloids like Reserpine and recinnamine present in it and possess potent tranquilizing, sedative and anti-hypertensive actions and used for various CNS disorders. Objective: Various marketed formulations containing rauwolfia as active constituent are available in market. As the adulteration is increasing day by day, evaluation and standardization of the dosage form is necessary to quantify the active constituents and physic-chemical. So it was effort to evaluate and standardize the marketed formulations of Rauwolfia for its Reserpine content. Material & Method: Three marketed formulation of rauwolfia were evaluated for its Reserpine content and standardized by macroscopic and microscopic evaluation, phytochemical and physicochemical evaluation and performed the TLC of extracts. Total alkaloids (calculated as reserpine) from various samples of Rauwolfia serpentine root powder and its marketed formulation were estimated by spectrophotometric method using ionpair complexation of acid dye (methyl orange) with the alkaloids. Result: The market formulations were complied with standard value of Physico-chemical parameters. Results of phytochemical investigation had shown the presence of alkaloid as Reserpine. The total alkaloids (% w/w) of Sarpagandha formulations A, B and C were found 0.3, 0.11 and 0.23 respectively. It is also found that the properties and contents are variable with each other. Keywords: Rauwolfia, Reserpine, Sarpagandha P-095: HPTLC FRINGERPRINTING & ANTI-HYPERLIPIDEMIC ACTIVITY OF GUAZUMA ULMIFOLIA LAM. LEAVES Chelli P. Israni, Dewanshi B. Mehta, N.M. Patel, A. A. Patel Shri B. M. Shah College of Pharmaceutical Education & Research, Modasa, Gujarat Guazuma ulmifolia Lam.plant known as Bastard cedar, family Sterculiaceae, was selected for HPTLC fingerprinting and anti-hyperlipidemic activity using high fat diet fed model in rats. HPTLC fingerprinting of Guazuma ulmifolia leaf extract for tannin, flavanoids and glycoside was carried out. Aqueous extract shown significant antihyperlipidemic potential towards high fat diet at 150, 250 and 500 mg/kg dose. In conclusion, HPTLC fingerprinting of most constituents of Guazuma ulmifolia leaves are soluble in water, acetone and alcohol and aqueous extract of Guazuma ulmifolia leaves decrease elevated serum VLDL. VLDL, LDL, triglycerides, atherogenic index, while increase serum HDL and HDL level in hyperlipidemic rats in dose-dependent manner. Keywords: Guazuma ulmifolia, HPTLC, anti-lipidemic activity P-096: PHARMACOGNOSTICAL, PRELIMINARY PHYTOCHEMICAL AND ANTHELMINTIC STUDIES ON QUISQUALIS INDICA LEAF Vidita Bhargava, Krupa Kalaria, Kruti Desai, Shilpa Bamania, Kishor Dholwani, Ajay Saluja Department of Pharmacognosy, A.R.C.P., Vallabh Vidyanagar The plant is easy to grow and can be planted in containers as ornamental vine. It consists of dried leaves of Quisqualis indica Linn. (Combretaceae). The plant Quisqualis indica has been reported traditionally to treat helminthiasis in Indian subcontinent and very less work has been reported on it so, it was decided to carryout pharmacognostical, phytochemical and pharmacological studies with special emphasis on anthelmintic activity on this plant. The leaf of the plant was evaluated for complete pharmacognostic parameters such as macroscopy and microscopy. Proximate analysis was also done which included moisture content, foaming index, ash values and extractive values, and elemental analysis. Preliminary phytochemical screening of various successive extracts of leaves revealed the presence of carbohydrates, flavonoides, sterols and phenolic compounds. Methanol and aqueous extract of leaf of Quisqualis indica were used for pharmacological studies. Among two extracts of leaf of Quisqualis indica Linn. aqueous extract showed more potent anthelmintic activity. This action of leaf of Quisqualis indica Linn. can be attributed to the phytoconstituents like flavonoids and saponins present in it. P-097: EVALUATION OF ANTIFUNGAL ACTIVITY OF OCIMUM SANCTUM LEAVES AGAINST HUMAN PATHOGENIC DERMATOPHYTES Palande N A, Saluja A K A.R.College of Pharmacy, Vallabh Vidyanagar Dermatophyte is a group of fungi, pathogenic to humans and responsible for causing skin infection commonly known as Ringworm. The clinical manifestation is development of typical enlarging red rings with interdigital skin appearing macerated and soggy, patches of skin becoming itchy and red and finally becoming dry and scaly. Allopathic treatment available for ringworm leads to improvement in clinical manifestations but relapse is almost universal.Ocimum sanctum is described as antimicrobial herb in ancient ayurveda as well as in modern literature. In this study the aqueous extract of the leaves of Ocimum sanctum was used to evaluate its antifungal efficacy against the human pathogenic dermatophytes namely, Trichophyton rubrum, Trichophyton metagrophytes and Epidermophyton flocossum. The strains were obtained from MTCC, Chandigarh and maintained on Sabourd Dextrose Agar medium. The antifungal activity was investigated at various concentrations of 50mg, 100mg, 150mg, 200mg, 250 mg and 300mg, by modified agar well diffusion technique and the zone of inhibition reported. The minimum inhibition concentration studied by broth dilution technique
and thus reported. The antifungal activity of aqueous extract of leaves of Ocimum sanctum was compared with the market formulation of ketokonazole 2%. The results obtained were very effective as compared to the market formulation and can be utilized for further investigation for treatment of ringworm. Keywords: Antifungal, Dermatophytes, Ocimum sanctum P-098: STANDARDIZATION OF ARTHRUMPLUS CAPSULE - A POLYHERBAL FORMULATION Avani B. Patel, N.M. Patel, A. A. Patel, Jalpa R. Modh Shri B. M. Shah College of Pharmaceutical Education & Research, Modasa, Gujarat Evaluation is necessary to ensure quality and purity of the herbal product. Arthrum plus capsule' consists of four ingredients viz., Oleogum resin of aqueous extract of Boswellia serrata Roxb, Leaves of aqueous extract of Pluchea lanceolata C.B. Clarke, Leaves of aqueous extract of Vitex negundo Linn and Yograj Guggula powder. It is used in Rheumatoid arthritis, Osteoarthrosis, Gouty arthritis and Spondylitis. For evaluation of capsule containing single herb various parameters were tested. Results point out that Raw material of `Arthrum plus capsule'has passed through all Organoleptic, Physicochemical, Physical, and Phytochemical parameters. All three batches of `Arthrum plus capsule' were uniform in colour, size, weight, dissolution and disintegration time, moisture content, angle of repose, bulk and tap density etc. Estimation of marker compound indicates presence of Gallic acid, Piperine, Gingerol, Quercetin, guggulosterol, 11-keto-boswellic acid at different concentration in Raw material and `Arthrum plus capsule'. Keywords: Arthrum plus capsule, standardization P-099: THERAPEUTIC PROSPECTIVE OF APHANAMIXIS POLYSTACHYA AS AN ANTI-HIV AND ANTIOXIDANT Chaitra Narayan L*, Ravishankar Rai V Department of Studies in Microbiology, University of Mysore Aphanamixis polystachya (Wall.) Parker, vernacularly called "Amoora rohituka" is a medicinal plant belonging to the family of Meliaceae and appearing among Indian natural drugs used as remedy as an astringent and applied on swelling after a fall. In recent times anti carcinogenic ingredients are found from this plant. Free radical stress leads to tissue injury and progression of disease conditions such as arthritis, hemorrhagic shock, atherosclerosis, diabetes, hepatic injury, aging and ischemia, reperfusion injury of many tissues, gastritis, tumor promotion, neurodegenerative diseases and carcinogenesis. Safer antioxidants suitable for long term use are needed to prevent or stop the progression of free radical mediated disorders. Antioxidant activity of the leaf of Amoora rohituka was evaluated in a series of In vitro assay involving free radicals and reactive oxygen species. The extract exhibited its scavenging effect in concentration dependent manner on ABTS radical scavenging, superoxide anion radicals, DPPH radical scavenging and property of metal chelating and reducing power. The extract has shown considerably good value for total reducing power, total phenolics, flavanoids and flavanol content. The results obtained in the present study indicate that the methanol extract of Amoora rohituka can be a potential source of natural antioxidant. Research is progressing to characterize the antioxidant compounds in the Amoora rohituka leaf extract and their mode of action in imparting antioxidant activity P-100: IN VITRO ANTIOXIDANT ACTIVITY OF METHANOL EXTRACT OF CANTHIUM PARVIFLORUM Deepashree C.L*, Shubha Gopal Department of Studies in Microbiology, University of Mysore Canthium parviflorum, also known in Sanskrit as Gangeruki, Chayatinisha belongs to the family Rubiaceae. Plant pacifies vitiated kapha, diarrhea, fever, leucorrhea, worm infestation and general debility. The free radical scavenging potential of the methanol extract of Canthium parviflorum was studied by different antioxidant models. Free radicals are implicated for more than 80 diseases including Diabetes mellitus, arthritis, cancer, ageing etc. In the treatment of these diseases, antioxidant therapy has gained an utmost importance. Current research is directed towards finding naturally occurring antioxidant of plant origin. To understand the mechanisms of pharmacological actions, the In vitro antioxidant activity of methanol extract of leaves of Canthium parviflorum was investigated for the activity of scavenging superoxide anion radicals, lipid peroxidation assay. The extract was also assessed for its total reducing power, total antioxidant activity, total phenolic, flavanoid and flavanol content. In all the testing, a significant correlation existed between concentrations of the extract and percentage inhibition of free radicals by ABTS and DPPH radical assay, metal chelating, super oxide radical, reducing power and inhibition of lipid peroxidation. The results are clearly indicative that Canthium parviflorum is highly effective against free radical mediated diseases possessing IC50 value in g concentrations for all the assays tested. A purpose oriented research on the isolation and characterization of bioactive antioxidant molecule would certainly yield rich dividend in terms of furnishing novel biomolecule which would prove to be a strong antioxidant from the methanol extract of Canthium parviflorum. Keywords: Canthium parviflorum, antioxidant P-101: GLORIOSA SUPERBA- A CRITICALLY ENDANGERED PLANT OF JHARKHAND AND WEST BENGAL PK Mishra, Anjan Kumar Sinha Dept. of Botany, Vinoba Bhave University, Hazaribag Gloriosa superba is generally found in western ghat, parts of Tamilnadu and Kerela, It is critically endangered hence IUCN has placed it in "red data book" recently this species has been spotted for the first time in
Topchanchi area of Dhanbad, Chandan Kiari of Bokaro and Ichak of Hazaribag District and different lowland of Bankura District of West Bengal. This is first report of this endangered plant from Jharkhand. This plant has diverse use so far their medicinal properties are concerned. It is important to note that tribal population of Jharkhand uses this plant in different ways. Munda and Oraon tribe use to use tuber of this plant as antifertility agent. Santhals use tuber of this plant for abortion and the stem is used in syphilis. Decoction of leaf is used in asthma. Root of this plant is used in facilitating child birth. Reference of medicinal properties of this plant is also available in Charak Samhita and Bhavprakash Nighantu. Gloriosa superba is a commercially imperative medicinal plant which has diverse medicinal applications and eventually due to over-exploitation this plant is facing local extinction. It has been affirmed as endangered plant by IUCN and hence there is a pressing need to conserve the plant by in situ and ex situ multiplication in general and micropropogation in particular so as to meet the ever increasing demand from the industries P-102: HYPOLIPIDEMIC ACTIVITY OF DHATRINISHA CHURNA IN HIGH FAT DIET INDUCED HYPERLIPIDEMIC RATS Vishal R. Patel1, R. K. Patel2, Madhavi G. Patel3, A. N. Paranjape1 1Baroda College of Pharmacy, Parul Group of Institutes, Vadodara, Gujarat; 2SKCPER, Mehasana Dhatrinisha churna has been traditionally used in the Ayurvedic system of medicine and by traditional medical practices of India to treat hyperlipidemia. Our aim was to evaluate lipid lowering activity of water extract of Dhatrinisha churna in high fat diet induced experimental hyperlipidemic rats. The activity was assessed by two different methods studying the lipid profiles of serum of the control and drug-treated animals. The results of the study were subjected to analysis of variance followed by Dunnett's t-test for multiple comparisons. Values with P < 0.001 were considered to be significant. The total water extract of the formulation and its individual components when tested for antihyperlipidemic action, exhibited a potential activity in albino rats when compared to standard drugs. Dhatrinisha churna reduced bed cholesterol (TC, TG, LDL & VLDL) and increase good cholesterol (HDL). The results lend support to the traditional use of Dhatrinisha churna in the treatment of hyperlipidemia in lowering only bed cholesterol and increase good cholesterol which is beneficial. Keywords: Dhatrinisha churna, lipid lowering activity, HDL P-103: IN VITRO ANTIOXIDANT STUDIES AND FTIR ANALYSIS ON ETHANOLIC EXTRACT OF ZALEYA DECANDRA Gopalakrishnan V.K., Meenakshi P Karpagam University, Coimbatore, Tamil Nadu Antioxidants have been reported to prevent oxidative damage and may prevent the occurrence of disease and aging. Antioxidants have the capacity to quench free radicals. Zaleya decandra is a prostrate weed belonging to the family Aizoaceae. It is distributed in the tropical and subtropical regions of the world. The root is used for the treatment of hepatitis, asthma, orchitis and the root's bark is credited with properties of aperients. In the present study, the antioxidant activity of the ethanolic extract of Zaleya decandra root and FTIR analysis were investigated. The Total phenols, flavonoids, carotenoids, tannins content were assayed. The antioxidant activity was determined on the basis of scavenging activity of the stable 1,1- diphenyl-2-picryl-hydrazyl (DPPH), Reducing power assay, hydroxyl scavenging activity, nitric oxide scavenging and superoxide radical scavenging activity. The results showed the presence bioactive compounds such as phenols, flavonoid, carotenoids and tannins. It also showed potent free radical scavenging activity and significant antioxidant activity of the plant extract. The FTIR analysis of the ethanolic extract of Zaleya decandra revealed absorption bands specific for various functional groups. Keywords: Antioxidant, Free radicals, Zaleya decandra P-104: LIPID LOWERING ACTIVITY OF FERONIA LIMONIA LINN. LEAF IN TRITON WR-1339 INDUCED HYPERLIPIDEMIA IN RATS Kanika Pandit, A M Bhagwat NMIMS University, Mumbai Back ground and objective: Hyperlipidemia is one of the greatest risk factors contributing to prevalence and severity of cardiovascular diseases. Hyperlipidemia is defined as an increase in lipid content in blood. Feronia limonia is a moderate sized deciduous tree grown throughout India. The bark and leaves of the plant are used for vitiated conditions of vata and pitta. The Fruit pulp of Feronia limonia have been reported to have antidiabetic, wound healing, antioxidant activities while the leaves have hepatoprotective activity. The present study was undertaken to investigate the antihyperlipidemic activity of methanolic leaf extract in triton induced hyperlipidemia in rats. Methods: Hyperlipidemia was induced by administration of single dose of Triton WR 1339 (400mg/kg, i.p) to rats. The methanolic extract of Feronia limonia leaf (125mg/kg, 250mg/kg and 500mg/kg body weight) was orally administered in hyperlipidemic rats. The degree of protection was determined by measuring levels of serum TG, TC, HDL-C, LDL-C, and VLDL-C levels. Atorvastatin (30mg/kg p.o) was used as references standards in triton WR1339 induced hyperlipidemic model. Results: Hyperlipidemia was evidenced by elevated levels of serum TG, TC, LDL-C, VLDL-C. The study demonstrated that after administering Feronia limonia leaf extract at different doses (125mg/kg, 250mg/kg, and 500mg/kg), a remarkable reduction in the total cholesterol, triglycerides, VLDL and LDL levels in the serum of hyperlipidemic rat was witnessed, suggesting a beneficial modulatory influence on cholesterol metabolism and turnover.
Conclusion: Feronia limonia exhibited antihyperlipidemic activity in Triton WR-1339 induced hyperlipidemia in rats. Keywords: Hyperlipidemia, Feronia limonia, Triton WR 1339, Antihyperlipidemic P-105: ANTIOBESITY AND ANTIHYPERLIPIDEMIC EFFECTS OF HIBISCUS ROSA SINENSIS LINN. LEAF EXTRACT ON HIGH FAT DIET INDUCED OBESE RATS Ritu Mishra, A M Bhagwat NMIMS University, Mumbai The global prevalence of obesity is increasing rapidly among adults as well as among children. It is generally recognized that natural products with a long history of safety can modulate obesity. The present study was aimed to investigate the development of obesity in response to a high fat diet and to estimate the effect of standardized dose of Hibiscus rosa sinensis leaf extract (HLE) on bodyweight, food intake, lipid profiles, hepatic function markers and glucose in blood. Albino Wistar rats were fed a high fat diet (HFD) for 40 days during the entire study. Rats of the HFD group were equally divided into 4 groups. Orlistat and atrovatatin was used as standard at a dose of 30mg/kg each. HLE administration at a dose of 500mg/kg body wt. significantly reduced food intake and body weight in obese rats, simultaneously improving the lipid profile. Serum ALT and AST levels, along with serum glucose were lowered significantly by HLE extract. Treatment with HLE extract improved obesity. Also HLE has hypolipidemic effects. Keywords: Anti-obesity, antihyperlipidemic, Hibiscus rosa sinensis, high fat diet P-106: PHYTOPHARMACOLOGICAL EVALUATION OF SOLANUM SISYMBRIIFOLIUM LAM. FRUITS WITH SPECIAL REFERENCE TO ITS ANTI-DEPRESSION AND ANXIOLYTIC ACTIVITY 1Vadnerkar KP, 2Vadnekar VC, 1Saluja AK 1A.R.College of Pharmacy, Vallabh Vidyanagar; 2Dept of Pharm. Science, Saurashtra Uni., Rajkot The present study was aimed to evaluate anti-depressant and anxiolytic activity of the ethanolic extract of Solanum sisymbriifolium using forced swimming test (FST) and Elevated plus maze (EPM) test in male wistar strain of rat respectively. Solanum sisymbriifolium (Family: Solanaceae) commonly known as Ubhi-bhoy ringani. It has been traditionally used as Diuretic, Hypotensive, Emenagogue, Fertility regulator and CNS depressant/stimulant. Depression and Anxiety are psychiatric disorders, characterized by a significant change in mood, thinking, behavior, and physiological activity. The powdered sample of fruit of S. sisymbriifolium was extracted with ethanol to yield ethanolic extract (95%) which used to evaluate the anti-depressant and anxiolytic activity on various animal models. The study revealed that the ethanolic extract of S.Sisymbriifolium at dose of 100,200,400 mg/kg significantly (p<0.001) reduced the mobility period in dose-dependent manner than the standard drug Imipramine (12.5 mg/kg, i.p) in forced swim test and increased the time spent in open arm as well as no. of total entries in open arm in dose dependent manner than the standard drug Diazepam (5 mg/kg, i.p.) in Elevated plus maze test, indicating powerful anti-depressant and anxiolytic activity. These finding suggested that the anti-depressant and anxiolytic effect of extract is specific. The result of these studies indicated usefulness of S. sisymbriifolium in the treatment of depression and anxiety. Keywords: Solanum sisymbriifolium, CNS activity P-107: BIOACTIVE COMPOUNDS FROM HIMALAYAN MEDICINAL PLANTS Roshni Bhatt, Gupta Arti, Sonia Pandey, Bhrugesh Joshi Uka Tarsadiya University, Bardoli, Gujarat Indian Himalayan region represents one of the richest sources of biological diversity in the world and has been a major source of herbal raw materials to the national and international markets. The indiscriminate and over exploitation of wild population of medicinal and aromatic plants, climatic changes and environmental factors in Indian Himalaya are responsible for their endangerments. The shifts of consumer choice and preference for herbal drugs, cosmetics agrochemicals than their synthetic counterparts have enforced new pressures on medicinal and aromatic plants of Indian Himalayan region. Various bioactive compounds have been isolated from medicinal and aromatic plants from different location and altitude of Indian Himalaya. The systematic activity guided isolation of bioactive compounds and development of molecular markers for elite population and germplasm are essential for organized cultivation of medicinal plants. A correlation of biological and chemical diversity is essential for the reproducibility of the biological activity in medicinal plants. The bioprospecting of endangered medicinal plants from Indian Himalaya are essential for the development of new drugs by activity guided isolation of active compounds, in combination of rational combinatorial chemistry and computational drug design. Keywords: Medicinal plants, Bioactive compounds, Activity-guided isolation P-108: STANDARDIZATION OF PANCHKOLA CHURNA AND SIMULTANEOUS ESTIMATION OF PIPERIN & PLUMBAGIN BY HPLC METHOD Viral G. Patel*, Nikunjana A. Patel S. K. Patel College of Pharmacetical Education & Research, Ganpat Universtiy, Kherva, Gujarat Objective: The aim of investigation was to evaluate the parameters of the Panchkola churna. Which contains sunthi, pipalimula, pippali, chitrak, chvya. It is used in vatagulma (abdominal lump), arsa (piles), panduroga (anaemia), aruchi (anorexia, impaired appetite). Method: The simultaneous determination of active constituents from Ayurvedic churna (Panchkola churna) by HPLC it was aimed to developed the
standardization method and generated the standard values for the quality assessment of this churna. In standardization procedure investigation was done by phytochemical parameters like procurement of raw material, macroscopical and microscopical evaluation, physical evaluation (foreign matter, loss on drying, ash value, extractive value), chemical evaluation(TLC of active constituent present), phytochemical screening, estimation of composition (total alkaloids and glycosides, total phytosterol, total flavanoids, total saponin, total tannins and phenolics), HPLC of both raw material as well as panchkola churna. Pharmaceuticals parameters (powder finesse, angle of repose, tapped density and bulk density, carr's index and hausners ratio, assay for active constituents) were investigated for churna. Results and Discussion: Results of standardization of Panchkola churna were as compared to standard active components on HPLC method and also compared with orgenoleptic parameters, physical parameters, chemical parameters, estimation of composition, pharmaceutical parameters. Conclusion: Panchkola churna contain active components piperin, plumbagin, zingerol and also glycoside, saponin, tannin, alkaloids, steroids in their raw materials. These constituents may be responsible for various activities. This data is also important for commercial purpose. Keywords: Panchkola churna, Piperin, Plumbagin, Simultaneous estimation P-109: EFFECT OF FAGONIA CRETICA LINN IN THE IN VITRO INHIBITION OF CHOLESTEROL CRYSTALS Poorvesh M. Vyas1, Lalit D. Chriya2, Viral B. Mandaliya2, Vrinda S. Thaker2 and Mihir J. Joshi1 1Physics Department, Saurashtra University, Rajkot; 2Bioscience Department, Saurashtra Uni Cholesterol is a multifunctional sterol molecule and has a role in the regulation of protein activity, including signaling protein in the brain. High level of cholesterol in blood can damage the blood vessels and may cause cardio vascular diseases. It is also a major constituent of gall stones. Therefore, it is interesting to study the growth inhibition of cholesterol crystals by herbal extract. This In vitro study has been carried out in the presence of the ethanolic extracts of Fagonia cretica Linn. using single diffusion gel growth technique. Sodium metasilicate solution of specific gravity 1.05 and 1M solution of acetic acid were mixed to set pH 5.0. After the gelation, supernatant solutions comprising of 1% cholesterol (in ethanol) mixed with the extract of Fagonia cretica Linn. were poured gently on the set gels. The control tubes were lacking herbal extract. The dimensions of growing crystals were measured up to for 48 h at the interval of 1 h by using travelling microscope. It was found that Fagonia cretica Linn. inhibits the growth of the crystals. This study may be used for formulating the therapeutic strategy for dissolution of cholesterol. Keywords: Cholesterol, Fagonia cretica Linn., Growth & Inhibition P-110: IMMUNOMODULATORY ACTIVITY OF BARK OF THESPESIA POPULNEA LINN. Jitendra B. Kandale, Shilpa Jagdale, Maruti Shelar MCE'S Allana College of Pharmacy, Pune Immunomodulatory activity of petroleum ether, ethyl acetate, alcoholic and aqueous extracts of Thespesia populnea was evaluated by using haemagglutination antibody titre, delayed type hypersensitivity reaction and cyclophosphamide induced myelosuppression in Swiss albino mice. Thespesia populnea showed effective increase in haemagglutination antibody titre, delayed type hypersensitivity reaction and was found active in normalizing WBC levels in case of cyclophosphamide induced myelosuppression in mice. Present study, therefore reveals that the drug holds promise as immunomodulatory agent, which acts by stimulating both humoral as well as cellular immunity. Keywords: Thespesia populnea Linn.; immunomodulatory activity P-111: PHYTOCHEMICAL AND PHARMACOLOGICAL EVALUATION OF FICUS LYRATA LEAVES EXTRACT Agrawal Kuldeep, S.Vikram, Ch.V.Rao, Agrawal Narottam Das, Pant Rahul Sri Rawatpura Intitute of Pharmacy, Datia (M.P.) Gastroprotective effect of Methanolic extract of F. lyrata Leaves (FLE) was studied in different gastric ulcer models in rats. FLE (100 and 200 mg/kg body weight) was administered orally, twice daily for 5 days for prevention from pylorus ligation (PL),ethanol (EtOH) and cold restraint stress (CRS) induced ulcers, Asprin induced ulcers(ASP) in rats.FLE showed dose dependent inhibition of ulcer index in pylorus ligation, ethanol and cold restraint stress induced ulcers. FLE prevents the oxidative damage of gastric mucosa by blocking lipid peroxidation and by significant decrease in superoxide dismutase, H+K+ATPase and increase in catalase activity. Keywords: Ficus lyrata, ranitidine, aspirin, Antiulcer activity P-112: EVALUATION OF DIURETIC ACTIVITY OF ETHANOLIC EXTRACT OF ACORUS CALAMUS LINN. RHIZOME Ghodasara JV, Chapala MV, Kyada AV, Rachchh MA, Jadav PD S.J. Thakkar Pharmacy College, Rajkot Objective: To evaluate diuretic activity of ethanolic extract of Acorus calamus Linn. rhizome (EEAC) in rats. Material and methods: Preliminary phytochemical screening of EEAC was conducted to identify the nature of phytoconstituents present in it. Rats were divided into five groups (n=6) namely control (0.5 % w/v CMC, p.o.), standard (Furosemide, 15mg/kg, p.o.), test 1 (EEAC, 250 mg/kg, p.o.), test 2 (EEAC, 500 mg/kg, p.o.) and test 3 (EEAC, 750 mg/kg, p.o.). Urine volume was measure after 6 hours by using metabolic cage. Amount of sodium and potassium ions were also estimated in urine of animal by using flame photometry. Results: Preliminary phytochemical screening of EEAC showed the presence of alkaloids, flavones, steroids, tannins, terpenoids and
saponins. Treatment with a dose of 750 mg/kg EEAC (3.78±0.11) revealed significant improvement in urine output as compared to control (1.88±0.18) animals. There were also increase in excretion of sodium (82.47±3.80) and potassium (12.06±0.47) ions in EEAC (750 mg/kg) as compared to control group. Conclusion: The preliminary phytochemical screening revealed that flavanoids, saponins, terpenoids, alkaloids were present in EEAC. These phytoconstituents of EEAC might be acting synergistically or individually to produce diuresis. The ethanolic extract of Acorus calamus Linn. Rhizome possessed significant diuretic activity which may be due to synergistic effect of above mentioned phytoconstituents. Keywords: Acorus calamus, diuretic activity, electrolyte concentration, furosemide P-113: QUANTITATIVE ESTIMATION OF PHYTOCHEMICALS AND ANTIOXIDANT ACTIVITY OF ROOTS OF JASMINUM SAMBAC Sengar N., Joshi A., Hemalatha S., Joshi V.K. Department of Pharmaceutics, Institute of Technology, Banaras Hindu University Jasminum sambac is an ornamental plant belonging to family Oleaceae. It is widely distributed throughout tropical Asia including India. On preliminary phytochemical investigation it was observed that the root part of the plant contained steroids, saponin, tannin, phenols, flavonoid and the presence of saponin, tannin, phenol and flavonoid was then validated by quantitative analysis. Total phenols estimated in the plant material was 5.63mg/g equivalent to gallic acid while total tannin was found to be 2.8mg./g equvalant to tannic acid which were observed to be quite low. The results also depicted a low content of flavonoids and flavonols i.e.33.05 mg/g and 0.62mg/g equivalent to rutin. However, the results demonstrated a very high saponin content of 106.25mg/g equvalant to diosgenin. The antioxidant activity is attributed to various chemical constituents like phenols, tannins, flavonoid, saponins etc. Sometimes biological reactions or exogenous factors due to physiological functions in body result in formation of free radicals such as reactive oxygen species including superoxide radicals, hydroxyl radicals, singlet oxygen and hydrogen peroxide. Excess of free radicals in body or deficiency of physiological antioxidant protective mechanism leads to oxidative stress which is one of the major causes of several diseases. The present work deals with determination of antioxidant potential of various extract of the root of Jasminum sambac using In vitro antioxidant models viz. assay of reducing power, antioxidant capacity by phosphomolybdenum method, scavenging of hydrogen peroxide and free radical scavenging activity. Keywords: Quantitative estimation, free radical, Antioxidant activity P-114: PRELIMINARY PHYTOCHEMICAL SCREENING AND ANTIOXIDANT POTENTIAL OF ROOTS OF ALBIZZIA LEBBECK (L.) BENTH. Joshi A., Sengar N., Hemalatha S., Joshi V.K. Department of Pharmaceutics, Institute of Technology, Banaras Hindu University Albizzia lebbeck is one of the most important plants used in Ayurveda. Its root, bark, seed, flower are used in various ailments. Of them, its bark is considered as best anti poisonous. The roots of Albizzia lebbeck showed presence of tannin & phenol, flavonoid, saponin, steroids and glycosides on preliminary phytochemical screening. Further quantification revealed that the roots contained sufficient quantity of saponin, tannin & phenol and flavonoid. Total phenols quantified in the roots was found to be 76.55mg/g equivalent to gallic acid while total tannin reported was 64.46mg./g equvalant to tannic acid The estimated value of total flavonoids and flavonols was observed to be 28.78 mg/g and 1.15 mg/g of dried extract equivalent to rutin. Total saponin content in the plant material was found to be 328mg/g equvalant to diosgenin. Tannin, flavonoids etc from plant source have been reported to have antioxidant property. Metabolism of oxygen in body yields reactive oxygen species (ROS) like superoxide radicals, hydroxyl radical and hydrogen peroxide molecule as by product of biological reactions. These ROS are the main factors involved in several degenerative disease. In order to alleviate the oxidative stress, antioxidants have important role either acting as antioxidant or as a scavenger. In the present work In vitro antioxidant models i.e. assay of reducing power, antioxidant capacity by phosphomolybdenum method, scavenging of hydrogen peroxide and free radical scavenging activity were used to determine the antioxidant potential of various extracts of roots of Albizzia lebbeck. Keywords: Albizzia lebbeck, Preliminary phytochemical screening, free radical, antioxidant activity P-115: PREPARATION AND EVALUATION OF POLYHERBAL FORMULATION FOR THE MANAGEMENT OF OSTEOPOROSIS Payal Suthar, Niranjan Kanaki, Chaitanya Bhatt, Vinit Movaliya, Sonal Patel, Maitreyi Zaveri Department of Pharmacognosy, KBIPER, Gandhinagar Osteoporosis is a systemic skeletal disease, characterized by low bone mass and micro architectural deterioration of bone tissue, leading to enhanced bone fragility and a consequent increase in fracture risk. There is no proper medication available for the cure of osteoporosis in allopathic system. Extracts of various indigenous medicinal herbs were screened for their inhibitory effect on bone resorption using In vitro bone culture experiment. The herbs showing significant activity in the In vitro screen were combined to prepare a poly herbal capsule formulation for management of osteoporosis. P-116: SCREENING OF THE INDIGENOUS MEDICINAL PLANTS FOR CHOLESTEROL ESTERASE INHIBITION ACTIVITY Dhara Bhatt, Niranjan Kanaki, Chaitanya Bhatt, Vinit Movaliya, Sonal Patel, Maitreyi Zaveri
K.B. Institute of Pharmaceutical Education & Research, Gandhinagar In recent years, much attention has focused on the inhibition of Cholesterol esterase as a potential target particularly for the development of hypocholesterolemic agents. Cholesterol esterase has three proposed functions, to control the bioavailability of cholesterol from dietary cholesterol esters; to contribute to incorporation of cholesterol into mixed micelles; and to aid in transport of free cholesterol to the enterocyte. Cholesterol esterase hydrolyzes cholesteryl ester to cholesterol. Inhibitors of Cholesterol esterase are anticipated to limit the absorption of dietary cholesterol. Screening of the indigenous medicinal plants for Cholesterol esterase inhibition activity was done using the colorimetric assay. Our aim of the work was to find the plants having Cholesterol esterase inhibition activity. The results will be discussed during the presentation. P-117: EVALUATION OF ACETYLCHOLINE ESTERASE IINHIBITORY ACTIVITY OF SELECTED MEDICINAL PLANTS Kunjal Vegad, Chaitanya Bhatt, Vinit Movaliya, Sonal Patel, Maitreyi Zaveri, Niranjan Kanaki Dept. of Pharmacognosy, K.B. Institute of Pharmaceutical Education & Research, Gandhinagar Acetylcholinesterase inhibitors (AChEI) are currently still the best available pharmacotherapy for the treatment of various neurological disorders such as Alzheimer's disease, senile dementia, ataxia and myasthenia gravis. It is also widely used for the treatment of paralytic ileus and glaucoma. Two colorimetric screening assays frequently used for screening and search for new AChEI, namely a thin layer chromatography (TLC) assay with Fast Blue B salt as reagent and a 96-well plate assay based on Ellman's method. In present work methanolic extract of Ficus religiosa, Nordastachys jatamansi, Semecarpus anacardium,Tinospora cordiflia and Withania somnifera were screened for the AchEI activity by Ellman's method. Bioactivity-guided fractionation of the active extract was done for the plant extract with good AchEI, in order to isolate active compound. The results will be discussed at the time of presentation. P-118: BIOACTIVITY GUIDED ISOLATION OF ANTI-DERMATOPHYTIC PRINCIPLES FROM COCONUT SHELL Devangi Pethani, Chaitanya Bhatt, Vinit Movaliya, Sonal Patel, Maitreyi Zaveri, Niranjan Kanaki K.B. Institute of Pharmaceutical Education & Research, Gandhinagar Dermotophytosis is widespread and increasing in prevalence on a global scale in ongoing period. Dermatophytes are a common label for a group of three types of fungus that commonly causes skin disease in animals and humans. The oil obtained from coconut shell has been used in Ayurvedic system of medicine for dermatophytic infections. The coconut shell extract has also been proved scientifically to have significant antidermatophytic activity. But no efforts have been done so far to isolate active compound from it. Alcoholic extract of Cocus nucifera (coconut) shell has been found to be effective for the treatment of dermotophytosis caused by Trichophyton, Epidermophyton and Microsporon species. Therefore, an attempt would be made to isolate anti-dermatophytic compound/s from coconut shell by bioactivity guided fractionation. Antidermatophytic activity will be evaluated using the Agar-well diffusion method for each fraction. The results will be discussed at the time of presentation. P-119: BIO-ACTIVE GUIDED FRACTIONATION OF OCIMUM BASILICUM FOR ITS NEPHROPROTECTIVE ACTIVITY Jigna Vadalia, Niranjan Kanaki, Chaitanya Bhatt, Sonal Patel, Maitreyi Zaveri, Vinit Movaliya Dept. of Pharmacognosy, K.B. Institute Of Pharmaceutical Education & Research, Gandhinagar Ocimum basilicum (family: Labiateae) is very well known herb mentioned in the category of "Pashanbheda". Yet, there is no attempt has been made to prove its nephroprotective activity. As per earlier report mentioned that hydroalcohol extract of Ocimum basilicum was active for its nephroprotective activity. Hence, in present study different fractions of hydroalcohol extract of aerial part of Ocimum basilicum will be studied for its nephroprotective activity in cisplatin - induced renal injury. In the cisplatin model, the different fractions such as petroleum ether, chloroform, ethyl acetate and methanol administered orally at different doses. The four fractions will be further studied for its antioxidant activity by using ABTS scavenging and DPPH free radical scavenging method. The results will be discussed at the time of presentation P-120: STANDARDIZATION OF SAMSHAMNI VATI Palak Soni, Niranjan Kanaki, Chaitanya Bhatt, Vinit Movaliya, Maitreyi Zaveri, Sonal Patel Dept. of Pharmacognosy, K.B. Institute of Pharmaceutical Education & Research, Gandhinagar Since ancient times, there has been exponential growth in the field of herbal remedies. Newer guidelines for standardization, manufacture, quality control and scientifically rigorous research are necessary for traditional medicines. Samshamni vati is an Ayurvedic formulation described in Bhaishajya Samhita and Ayurvedic pharmacopoeia and used for treatment of cough, fever, chicken gunia, swine flu, etc. It is Ayurvedic preparation containing four ingredients Piper longum, Tinospora cordifolia, Aconitum napellus and Loh bhasma. An attempt was made to standardize the formulation on the basis of microscopic, macroscopic and physical-chemical parameters and by using modern instrumental techniques like High Performance Thin Layer Chromatographic. The quality parameters and evaluation methods developed hereby can be used for routine quality control of Samshamni vati. The results will be discussed at time of presentation. P-121: PREPARATION AND EVALUATION OF POLYHERBAL SYRUP FOR TREATMENT OF PYREXIA Hetal Desai, Niranjan Kanaki, Vinit Movaliya, Sonal Patel, Maitreyi Zaveri, Chaitanya Bhatt
Dept. of Pharmacognosy, K.B. Institute of Pharmaceutical Education & Research, Gandhinagar Pyrexia is one of the most common symptoms of almost all disease. Allopathic medicine like paracetamol and other antipyretic drugs are having serious side effects and hence should not be used for longer time. Here, efforts have been made to prepare antipyretic syrup using well documented herbal drugs like Andrographis paniculata, Cyprus rotundus, Piper longum, Tinospora cordifolia and Solanum xanthocarpum. The antipyretic activity of proposed polyherbal formulation would be evaluated in endotoxin-induced pyrexia in rabbits. Various extracts like water, alcohol and hydroalcoholic extracts at different dose would be evaluated and the dose of particular extract that gives highest antipyretic activity would be selected for the preparation of polyherbal syrup. The formulation is expected to show very significant reduction of endotoxin-induced pyrexia in rabbits with respect to control group. The results will be discussed at time of presentation. P-122: TO PREPARE AND EVALUATE POLYHERBAL FORMULATION FOR THE MANAGEMENT OF NEPHROPROTECTIVE ACTIVITY Naiya Patel, Niranjan Kanaki, Chaitanya Bhatt, Sonal Patel, Maitreyi Zaveri, Vinit Movaliya Dept. of Pharmacognosy, K.B. Institute of Pharmaceutical Education & Research, Gandhinagar Since, there is no proper treatment available in allopathic system of medicine for kidney stone disease. Patient has to rely on herbal or Ayurvedic medicine for the management for the same. Many herbs are scientifically evaluated for their efficacy along with their mechanism of action and these selected herbs act through different mechanism. Hence, it was expected that the polyherbal formulation prepared using combination of different herbs might be used as nephroprotective drugs. Bhoomiamlaki (Phyllanthus amarus), Apamarg (Achyranthes aspera), Chhota gokhru (Tribuluas terrestris) and Punarnava (Borehaavia diffusa), which are used in Ayurveda for nephroprotective activities and having different possible mechanisms, to give synergetic nephroprotective activities. Hence, newly developed herbal formulation will be found to be very effective for the management of nephroprotective activities. P-123: SCREENING OF IN VITRO ANTIMALARIAL ACTIVITY OF SOME SELECTED PLANT EXTRACTS AND THEIR COMBINATIONS Shailesh Rana, Niranjan Kanaki, Vinit Movaliya, Sonal Patel, Maitreyi Zaveri, Chaitanya Bhatt K.B. Institute of Pharmaceutical Education & Research, Gandhinagar Malaria has become a major public health problem mainly due to the development of resistance by lethal causative parasitic species, Plasmodium falciparum towards the classical allopathic anti malarial agents. There are many Ayurvedic herbs which act as antimalarial and have different mechanisms of action. An attempt was made to evaluate the antimalarial activity of the various herbs alone and in combinations with each other to determine the presence of synergism. Traditionally used herbs such as Calotropis procera, Andrographis paniculata, Clerodendron inermi, Ocimum sanctum, Piper nigrum were evaluated in combination for synergistic antimalarial effect by schizont maturation inhibition assay. The synergy was determined by isobologram method. The results will be discussed at the time of presentation. P-124: SCREENING OF IN VITRO ANTIMALARIAL ACTIVITY OF SOME SELECTED PLANT EXTRACTS AND THEIR COMBINATIONS Robin Macwan, Niranjan Kanaki, Vinit Movaliya, Sonal Patel, Maitreyi Zaveri, Chaitanya Bhatt K.B. Institute of Pharmaceutical Education & Research, Gandhinagar Increase in resistance of Plasmodium faciparum towards available allopathic medicines and their dose dependent toxicity has lead to study plants for developing new antimalarial drugs.There are many herbs used traditionally which have anti malarial activity with different mechanism of action.Here an attempt was made to evaluate the antimalarial activity of the various herbs alone and in combinations with each other to determine the presence of synergism. Alstonia scholaris, Azadirachta indica, Curcuma longa, Caesalpinia bonducella, Datura metel which are used in Ayurveda for antimalarial activities and having different possible mechanisms, were selected to give synergistic antimalarial activity. This activity was checked by schizont maturation inhibition assay. The synergy was determined by isobologram method. The results will be discussed at the time of presentation. P-125: ANTIMICROBIAL AND HEMOLYSIS STUDY OF PURE AND ZINC DOPED NANO-HYDROXYAPATITE Kashmira P. Tank, Kiran S. Chudasama, Vrinda S. Thaker*, M. J. Joshi Department of Physics, Saurashtra University, Rajkot Hydroxyapatite (Ca10(PO4)6(OH)2 or HAP) is biologically most important compound, as it is the primary mineral in hard tissues such as bones and teeth. In the present study, zinc doped nano-hydroxyapatite (Zn-HAP) was synthesized via chemical precipitation route using surfactant mediated approach. The doping of zinc was confirmed by EDAX. The powder XRD pattern revealed the typical hydroxyapatite pattern with typical broadening and the presence of extra phase was observed for higher concentration of Zn doping. The average crystallite size was calculated by applying the Scherrer's formula to powder XRD pattern and was found in the range of 16 to 33 nm. The morphology of synthesized nano-particles was also confirmed using TEM. The antimicrobial activity was evaluated against two bacteria Pseudomonas aeruginosa as Gram negative and Micrococus luteus as Gram positive. The Hemolytic test result suggested that all the samples are non-hemolytic. Keywords: Nano-Hydroxyapatite, XRD, TEM, Antimicrobial Study
P-126: EFFECT OF RASAYAN CHURNA IN EXPERIMENTAL CIRRHOTIC ACSITES- A POSSIBLE HOPE FOR HEPATORENAL FAILURE Viral Desai 1, Rakesh K.Patel2 1Baroda College of Pharmacy, Limda; 2 S.K.P.C.P.E.R., Ganpat University, Kherva, Gujarat Cirrhotic ascites or Hepatorenal syndrome (HRS) is a life-threatening medical condition that consists of rapid deterioration in kidney function in individuals with cirrhosis or fulminant liver failure. HRS is usually fatal unless a liver transplant is performed, although various treatments, such as dialysis, can prevent advancement of the condition. It is a relatively common complication of cirrhosis, occurring in 18% of cirrhotics within one year of their diagnosis, and in 39% of cirrhotics within five years of their diagnosis. Rasayana churna is an ayurvedic formulation made up equally of Guduchi (Tinospora coridifolia), Gokshur (Tribulus tereteris), and Amalaki (Phyllanthus emblica). It is well documented as effective in various liver and kidney pathologies. Its immunomodulatory and anti oxidant properties suggested us to investigate its effect in HRS. In our study experimental cirrhotic ascites is induced in the either sex of wistar rats by validated procedure. Groups of animals receiving rasayana churna and its separated three constituents in the form of water extract are kept under observation. Changes in the parameters like liver-kidney function tests (alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total bilirubin, and serum creatinine) with serum osmolarility, serum sodium, and serum potassium level were evaluated. After treatment anti-oxidant status of liver is evaluated by SOD, catalase, glutathione peroxidase, and glutathione reductase enzymes. The prolific data is analyzed with suitable statistical method. The results show prominent modulation on cirrhotic ascites by rasayana churna and its three constituent. The effect may be due to its properties like immunomodulation and anti-oxidant, which may be justifiable by detail investigation. Keywords: Cirrhotic ascites, Hepato Renal syndrome, Rasayana churna, Ayurveda P-127: PHARMACOGNOSTIC AND PHYSICO-CHEMICAL PARAMETERS OF CORDIA DICHOTOMA G. FORST STEM BARK Dewanshi B. Mehta, N.M. Patel, A. A. Patel, Chelli P. Israni Shri B. M. Shah College of Pharmaceutical Education & Research, Modasa, Gujarat Present study of Cordia dichotoma stem bark (Boraginaceae) known as motto gundo (Gujarati) was carried out for its pharmacognostic study and physic-chemical parameters. Pharmacognostic and physic-chemical parameters like ash and extractive values, pH, volatile and moisture content, foreign organic matter, swelling and foaming index. Successive extract of stem bark was prepared with increasing order of polarity of solvent and evaluated for phytochemicals. In results it was found that stem bark of plant was brittle, flat, slight recurved, slight longitudinal easily breakable. T.S. shown phellem, phellogen, cortex, cambium, secondary phloem, ground tissue, calcium oxalate crystals and starch grains cell inclusions, lignified fibres as diagnostic characteristics. Sulphated ash>total ash was noted. Water has higher extractive matter while petroleum ether has less. Phytochemical investigation of petroleum ether and benzene extract had shown presence of phytosteroids, triterpenoids, fats and fatty oil. Chloroform and acetone extracts showed presence of fats and faay oil and tannins respectively.Alcohol and water extract showed tannins, flavanoids, triterpenoids, glycosides and saponins. Keywords: Cordia dichotoma, pharmacognosy, physico-chemical parameters P-128: EVALUATION OF CLASSICAL AYURVEDIC FORMULATION VARUNADI KWATH FOR ITS ANTILITHIATIC ACTIVITY BRIJAL ASARI, MADHURI SADHWANI, MEGHA SHAH, PRAVIN MESARIYA, VAISHALI SHAH APMC College of Pharmaceutical Education and Research, Himmatnagar, Gujarat Varunadi (VN) kwath a classical Ayurvedic formulation mention in Ayurvedic literature "Cakaradatta, Asmaricikitsa 12" for kidney stone. The presnt study investigated the Antilithiatic activity of kwath to validate the traditional use of this formulation. Kwath composed of Bergenialigulata rhizomes, Crataeva nurvala bark, Tribulus terrestris fruits, Zingiber officinale rhizome. Total phenolic & total flavonoid content in kwath were calculated by folinciocalteau method and AlCl3 colorimetric method.Kwath were evaluated for antiurolithatic activity by in vivo inWistar albinorats by Ethylene glycol induced urolithiasis. Oxalate,calcium,phosphate and magnesium were monitored in urine and kidney homogenate.Serum creatinine,Blood urea nitrogen and uric acid were also recorded.Total polyphenolic(40.5±0.13mg of gallic acid per gram of Kwath) and flavonoid compound (3.55±0.25 mg of rutin per gram of Kwath) were present in VN Kwath.In vivomodel, in calculi induced animals, oxalate. Calcium and phosphate in urine and kidney homogenate grossly increased which was significantly lowered (p<0.001) by Kwath treatment which was almost similar to cystone. There was increased serum creatinine, blood urea nitrogen, uric acid,in calculi induced rats were significantly(p<.001) lowered with Kwath treatment. Varunadi kwath has potent antilihtiatic activity upon EG induced nephrolithiasis in rat that may be due to synergestic effect of phytoconstituent present in kwath like triterpenoidlupeol, botulin, phenolic and flavonoids. P-129: ANTIOXIDANT AND ANTICANCER PRPOERTY OF SEMECARPUS ANACARDIUM EXTRACT IN B16F10 MELANOMA CELL LINES D. Kishore Ram Kumar, J. Arockia John Paul, D. Karthik Rajan, R. Balasubramanian, T. Starlin Sri Kaliswari College of Arts & Science, Sivakasi
Skin cancer is one of the major disorders caused due to radiations, pollutions and free radicals formed due them. Modern way of medicine is effective in treating it, but those are at very high expenses. Traditional method of treatment may be effective. Semecarpus anacardium is a plant which was used in many traditional medicine preparations and it is also found to be effective in treating various cancers. In our study we did trails in the effectiveness of S. anacardium nut extract against B16F10 melanoma cell lines and its antioxidant properties. From our studies we found that, S. anacardium nut crude methanolic extracts and its fractions showed a high antioxidant levels and this is evident through the free radical scavenging activity. The extract also showed a significant cytotoxicity against B16F10 melanoma cell lines. The hexane fraction and the crude methanolic extracts showed higher cytotoxic effect in the B16F10 melanoma cell lines. Similarly the S. anacardium extract treated cell lines analyzed for caspase and DNA fragmentation assay confirms the cytotoxic effect may be due to the induction of apoptotic pathway. In conclusion, the S. anacradium methanolic extract has a potential anticancer activity against B16F10 melanoma cell lines. The anticancer property may be due to its rich antioxidant contents and free radical scavenging activity. Keywords: Semecarpus anacardium, B16F10 melanoma cell lines, Antioxidant P-130: WONDERFUL ANTICANCER DRUG TAXOL Bhatt SN, Barad RA, Upadhayay SU, Ghule RS, Upadhayay UM Sigma Institute of Pharmacy, Bakrol, Vadodara Taxol is a naturally occurring diterpenoid chemical moiety present in various species of genus Taxus, belonging to family Taxaceae, viz. Taxus buccata (English Yew) leaves, Taxus brevifolia (Pacific Yew) bark, and others. It is usually cultivated upto an altitude of 2000-3500m. including Europe, Canada, South America, India (Kashmir). It is a crystalline odourless white powder with bitter taste, showing its solubility in organic solvents (Alcohol, Chloroform, Methylene dichloride) and insolubility in water. Its chemical formula has been found is C47H51NO14 with molecular mass of 853.906g/mol. It is a new anti-cancer compound possessing complex diterpenoid with a taxane ring system and four membered octane ring, along with ester side chain at position 13 of the taxane ring. The drug, also referred as Yew or Paclitaxel, promotes the polymerization and stabilization of tubulin to microtubules and interferes with the mitotic spindle, giving action by binding at betatubulin subunit. In short, it can be categorized as Microtubule stabilizing anticancer agent. In smaller doses, yew has been used as a minor folk medicine in some parts of its range, as traditionally, it have been claimed for induction of menstruation, as well as in the treatment of arthritis, kidney disease, scurvy, tuberculosis, and other ailments. USFDA has also approved its use in the treatment of refractory ovarian cancer. Clinical trials have been revealed that taxol is effective in the treatment of patients with refractory ovarian cancer, breast cancer, malignant melanoma and probably other solid tumors. It has also a promising role against carcinomas of lung, gastric, head, neck, prostate and colon. Toxicities of the drug include anaphylactoid reactions, leukopenia, peripheral neuropathy and oropharyngeal mucositis. Keywords: Taxol, microtubule stabilizer, anticancer agent, diterpenoid chemical P-131: CYTOGENETIC EVALUATION FOR CHEMOPROTECTIVE POTENTIAL OF SOME TRADITIONALLY USED HERB AS TONIC Varandani Deepa, Karchuli M.S., Patil U.K. People's institute of pharmacy & research centre, People's university, Bhopal Chemotherapy is always associated with side effects. Many researchers have proved significant protective potential of components from herbal origin against these chemotherapeutic agents induced mutagenencity. Since ancient time herbs are considered as rich source of medicine. Traditional knowledge of use of herbs in treatment of various ailments is still lacking exploration. Folk lore healers are using many herbs as tonic to rejuvenate body. In present study by ethnobotanical survey and literature survey few herbs were selected on the basis of their use as tonic. Effect of hydroalcoholic extracts of selected herbs were assessed on the basis of modulation of chromosomal aberration and microneucleas in bone marrow of Swiss albino mice due to cyclophosphamide administration. Each extract was given at 200mg/kg. It was observed that most of the selected herbs produced significant protection against Cyclophosphamide induced mutagenecity. Thus from study it can be concluded that herbs can work as a rich source for providing a potent chemoprotective agent. Keywords: Mutagenecity, Herbs, Cyclophosphamide, Bone marrow, tonic P-132: A RAY OF HOPE FOR HIV PATIENTS CALANOLIDES Patel HP, Atodaria U, Upadhyay SU, Ghule RS, Upadhyay UM Sigma Institute of Pharmacy, Bakrol, Vadodara New category of coumarin compounds named as Calanolides are isolated from the ripe as well as unripe seeds of various species of Calophyllum belonging to family Clusiaceae, viz. Calophyllum lanigerum, Calophyllum inophyllum and others. It is found generally in tropical Asia region and rain forests of the malaysia. Its chemical formula found is C22H26O5 and molecular mass 370.44g/mol. Calanolide A blocks transcription of the viral RNA into DNA within the host cell's cytoplasm with a triphenol. The absolute stereochemistry of calanolides A and B was established by a modified Mosher's method. Calanolides A and B were showing complete protective action against HIV-1 replication, but were inactive against HIV-2. Studies with purified bacterial recombinant reverse transcriptases (RT) revealed that the calanolides are HIV-1 specific RT inhibitors. (+) Calanolide A and (-) Calanolide B are the most potent candidates of anti-HIV-1agents among Calophyllum coumarins. Calanolide A is the only naturally occurring anti-HIV agent to be at an advanced stage of a phase II clinical trial, but it has
been obtained in relatively low yield isolated from Calophyllum lanigerum. Some structural modifications of (+)calanolide A or (-)-calanolide B have been carried out, but however, no compounds showed anti-HIV-1 activity superior to the two lead. The calanolides represent a substantial departure from the known class and therefore provide a novel new anti-HIV chemotype for drug development. Other minor uses of this class of drug include its anti-convulsant, anti-gout and anti-inflammatory activities. It have been found promising herb in treatment of HIV-1. Most common side effects of calanolides seen are headache, oily taste, dizziness, and heart burn. Keywords: Calanolides, anti-HIV agent, coumarin compound, reverse transcriptase P-133: CHROMATOGRAPHIC FINGER PRINT STUDIES OF STEROIDS IN ZANTHOXYLUM RHETSA ROXB (DC) BY HPTLC TECHNIQUE Alphonso Priya and Saraf Aparna Department of Botany, The Institute of Science, Mumbai Zanthoxylum rhetsa Roxb (DC) is widely used in ayurvedic preparations, therefore it is important to establish quality of the plant raw material for its constituent plant part composition. The aim of the study is to establish a chemical fingerprint profile and steroids composition of the medicinally important plant Zanthoxylum rhetsa Roxb (DC). Preliminary phytochemical studies were carried out by method as described by Harborne and Wagner. HPTLC studies were done by the method of Reich and Schibli. The solvent system employed was that of n-Butanol: Methanol: Water (3:1:1). The ethanolic extract of fruits of Zanthoxylum rhetsa Roxb (DC) showed the presence of 8 different steroids with Rf ranging from 0.03 to 0.92. The best separation of band was observed at 366 nm before derivatization with methanolic H2SO4. This fingerprint profile can be utilized to prevent adulteration and also act as a biochemical marker for this medicinally important plant by the pharmaceutical industry and plant systematic studies. Keywords: Steroids, HPTLC fingerprint, Zanthoxylum rhetsa Roxb (DC) P-134: IN-VITRO EVALUATION OF RADICAL SCAVENGING ACTIVITY OF DIFFERENT SUCCESSIVE LEAVES EXTRACTS OF PLUMERIA OBTUSA Pallavi S Adate1, Arun Kashid2, Amit Kute3 Sinhgad Institute of Pharmacy, Narhe, Pune The present study was conducted to investigate the free radical scavenging activities of different extracts obtained from Plumeria obtusa leaves by using different antioxidant capacity assays. Free radicals are the chemical entities that can exist separately with one or more unpaired electrons. The generation of free radicals can bring about thousands of reactions and thus cause extensive tissue damage. Lipids, proteins and DNA are all susceptible to attack by free radicals. Four successive extracts were obtained from solvents according to polarity like petroleum ether, chloroform, methanol and water. The results revealed that out of these four extracts methanolic and water extracts exhibited potent antioxidant activity and effectively scavenged free radicals in dose dependent manner from 50 to 250µg/ml. However petroleum ether extract showed least inhibition in all In vitro models. Keywords: Plumeria obtusa, Radical scavenging, Free radicals P-135: PRODUCT DEVELOPMENT AND STANDARDISATION OF AN ANTIPYRETIC POLYHERBAL FORMULATION Komal S. Patel, Nishit A. Gohel, Lal Hingorani, G. Prakash Yoganandam Dharmaj Degree Pharmacy College, Dharmaj (Anand), Gujarat In the present study, the four Ayurvedic herbals such as Swertia chirata (Gentianaceae), Nyctanthes arbor tristris (Oleaceae), Tinospora cordifolia (Menispermaceae) and Ocimum sanctum (Labiatae) claimed traditionally for antipyretic activity were chosen to develop a poly herbal formulation and it was named as "Herbocin". The detailed study includes selection of raw materials, authentication, preparation of aqueous and alcoholic extracts in spray dried form, preparation of herbal syrup and standardization of prepared herbal syrup using current and novel methods. It is generally realized that monitoring the quality of any herbs and its formulations, HPTLC fingerprinting is ideal, which involves comparison between a standard and a sample. By use of markers ensures that, the concentration and the ratio of components in the herbal mixture are present in reproducible levels in the raw material as well as in the final dosage forms. In this way use of markers and chromatographic fingerprinting can give information assisting manufacturing control and assuring bath to bath consistency. The formulation (Herbocin) shows significant (p<0.01) antipyretic activity in yeast induced pyrexia in rats. The activity being comparable to that of standard drug Paracetamol. This study provides the present pharmacological evidence and scientific support for the claim those ingredients in the formulation. An effort has been undertaken to check the hepatotoxicity. On the basis of the results it was clearly understood that developed formulation is three times less hepatotoxicity than the frequently used antipyretic agent i.e. Paracetamol. In a nutshell, this current research might give a path to develop and standardize an antipyretic polyherbal formulation with minimum or no hepatotoxic effect for the better clinical use with improved patient compliance. Keywords: Polyherbal formulation, HPTLC fingerprinting, Antipyretic activity P-136: ANTIFERTILITY ACTIVITY OF ETHANOL EXTRACT AND ITS FRACTIONS OF MENTHA ARVENSIS LEAVES IN FEMALE RATS
Sachin R. Patil1, M. B. Patil2, Sunil S. jalalpure1, Mukesh S. Sikarwar1 University College of Pharmacy, Belgaum; 2K.L.E.S's College of Pharmacy, Ankola, Karnataka Objective: To evaluate the anti-implantation and estrogenic activity of ethanolic extract and its fractions of Mentha arvensis leaves in female rats. Materials & Methods: The Acute oral toxicity study was performed according to OECD guidelines. The ethanolic extract and its fraction were investigated for anti-implantation and estrogenic activities at the dose of 200mg/kg body weight. Anti-implantation activity was studied in proven fertile female albino rats and given orally on days 1-7 of pregnancy. Estrogenic activity was assessed in immature female albino rats by taking uterine wet weight, vagina epithelial cell cornification, histological studies and on some of the estrogen dependent biochemical parameters in uterus. Result and Discussion: The ethanolic extract and its petroleum ether fraction exhibited 50% and 33.6% of anti-implantation activity when given orally at 200mg/kg body weight. The ethanolic extract and petroleum ether fraction also exhibited estrogenic activity by showing increase in uterine wet weight, vaginal epithelial cornification. Histological changes such as thickness of endometrium and myometrium was alerted in the treated rats. There were also increase in glucose cholesterol and alkaline phosphate levels when compared with the control rats. Keywords: Mentha arvensis, estrogenic, anti-implantation, Histological studies
P-137: AN ETHNOMEDICINAL STUDY ON TRADITIONAL REMEDY USED IN ARTHRITIS AND RELATED DISORDER BY MISHING TRIBE IN ASSAM Mahua Bhaumik Singha, Biswajit Singha Institute of Pharmacy, Gauhati Medical College, Guwahati, Assam Arthritis can present itself in many forms, and the most common complaint from patients with any form of arthritis is the persistent pain. The pain is caused by functional problem of the joints. Since the inception of civilization man has constantly searched for natural remedies for various disorders. Herbs possess enormous healing power, but only a part of it is known to mankind. Researchers are keen to discover the therapeutic use of the plant kingdom. Their effective and sustainable utilization would ensure protecting biodiversity and harnessing their healing power for the benefit of mankind. North-Eastern region of India is rich in flora and fauna. The Mishing are an ethnic tribe of Assam having their unique culture and custom. It has been generally observed that they are free from many diseases and lead healthier lives. A field study has been conducted in Dhemaji district of Assam on the Mishing tribe. They use the plaster, pill and message oil made of some locally available plants include Tinospora cordifolia (entire plant), Litsea salicifolia (leaves), Persea bombycina (leaves), Gossipium herbaceum (leaves), Ricinus communis (seeds), Moringa oleifera (bark), Sesamum orientale (seeds), Hibiscus rosasinensis (flower), Amphineuron extensus (leaves), Musa sapientum (leaves). After extensive interview and discussion with traditional healers, the common people and the patients, the method of preparation and application of the dosage forms of remedies for arthritis has been documented. Reference of the analgesic effect of the plants has been studied both in modern literature and ancient literature. The phytoconstitutents present in the plants have also been studied. Such an ethnomedicinal study will open new avenues in the field of research. A safe and effective use of the plants in arthritis can be discovered. Keywords: Arthritis, Mishing Tribe P-138: PHYOCHEMICAL SCREENING, ANTIOXIDANT ACTIVITY AND ANTIMICROBIAL ACTIVITY OF SPATHODEA CAMPUNALATA (STEM +BARK) EXTRACTS Meher, Ayesha Mateen, Irfan A Ghazi, M Z Gul and D. Manohar Rao Department of Genetics, Osmania University, Hyderabad, Andhra Pradesh Ethanolic, Methanolic and Aqueous extracts of Spathodea campunalata stem +bark were evaluated for phenolic content, flavanoid content, antioxidant and antimicrobial activities, free radical scavenging activity were evaluated by using phosphomolybdenum assay , 1,1-Diphenyl-2-picryl-hydrazyl( DPPH)radical method and by lipid The antimicrobial activity was evaluated using agar well diffusion assay protocol against eight bacterial reference strains, Staphylococcus aureus(ATCC 25923), Escherichia coli(ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), Proteus vulgaris (ATCC 6380), Salmonella typhimurium (ATCC 25241), Salmonella paratyphi (ATCC 9150), Shigella sonnei (ATCC 25931) and Klebsiella pneumonia (ATCC 27736). Results revealed that Methanolic extract showed higher polyphenol and flavonoids content, aqueous extract showed higher total antioxidant activity. The methanolic and ethanolic extracts exhibited better activity against eight bacterial strains used respectively compared with the aqueous extract. The extracts of Spathodea campunalata possess significant antioxidant activity. Keywords: Spathodea campunalata, extracts, antioxidant, antibacterial activity P-139: TOXICITY STUDY AND ANTINOCICEPTIVE ACTIVITY OF GMELINA ARBOREA EXTRACT AND ITS FRACTIONS Yogesh Kulkarni, Dhananjay Chavan SPP School of Pharmacy & Technology Management, SVKM's NMIMS, Mumbai Gmelina arborea (Verbenaceae) is an important medicinal plant in the traditional system of medicine in India. The present study was designed to evaluate acute toxicity and antinociceptive property of the alcohol extract (AlcE) of Gmelina arborea stem bark and its various fractions. The alcohol extract was prepared by Soxhlet extraction technique. It was fractionated with petroleum ether, chloroform, ethyl acetate and n-butanol. The acute toxicity of AlcE and its fractions was studied in female Swiss albino mice using the OECD Guidelines 423. In the acute toxicity test, mice were treated with AlcE and its fractions (2000 mg/kg), orally. Animals were
observed periodically during the first 24 h after administration of the test articles, and daily thereafter for 14 days. The antinociceptive activity was studied in Swiss albino mice by using acetic acid induced writhings method. The AlcE (250 and 500 mg/kg) and its fractions (200 mg/kg) were administered to animals before 45 minutes of administration of acetic acid. The number of muscular contractions was counted over a period of 20 minutes after acetic acid injection. AlcE and its fractions did not produce mortality, changes in behavior or any other physiological activities in mice, at selected dose. The AlcE at 250 and 500 mg/kg showed significant decrease in writhes -13.4 ± 0.16 and 12.2 ± 0.22 respectively when compared with control (19.2 ± 0.58). All fractions at selected dose showed significant decrease in number of writhes in experimental animals. The n-butanol fraction showed maximum inhibition of writhes (87%) as compared to other fractions. These findings suggested that AlcE and its fractions are safe after oral administration and have significant antinociceptive activity. Keywords: Gmelina arborea, toxicity, antinociceptive P-140: ANTIMICROBIAL EVALUATION OF SOME PHYTO-CHEMICALS OF WITHANIA SOMNIFERA Premlata Singariya*1, Padma Kumar 1 and Krishan Kumar Mourya2 1Department of Botany, University of Rajasthan, Jaipur; 2Govt. Vet.Hospital, Pahari, Rajasthan In recent times, there have been increases in antibiotic resistant strains of clinically important pathogens, which have led to the emergence of new bacterial strains that are multi-drug resistant. The non-availability and high cost of new generation antibiotics with limited effective span have resulted in increase in morbidity and mortality. Therefore, there is a need to look for substances from other sources with proven antimicrobial activity. We evaluated the antimicrobial (antibacterial and antifungal) effects of hexane, toluene, iso propyl alcohol, acetone and ethanolic extracts of different parts (flower, unripen and ripen fruits) of Withania somnifera (RUBL-20668). The dried and powdered parts were successively extracted using soxhlet assembly then antibacterial and antifungal activities were investigated with by both disc diffusion assay (DDA) and serial dilution methods. The extract of W. somnifera significantly inhibited some important bacteria (two Gram +ve and four Gram -ve bacteria). Staphylococcus aureus, Bacillus subtilis (Gram +ve), Escherichia coli, Raoultella planticola, Pseudomonas aeruginosa, Enterobactor aerogens, one yeast Candida albicans and one fungi Aspergillus flavus to varying degrees. Iso propyl alcohol, acetone and toluene extracts of W. somnifera showed highest activity by Minimum inhibitory concentration, Inhibition zone, Minimum bactericidal/fungicidal concentration, Total activity and Activity index (MIC, IZ, MBC/MFC, TA and AI) against the pathogens. The inhibitory effect is very significantly (P<0.005) in magnitude and comparable with that of standard antibiotics. Gentamycin and Ketoconazole, standard antibiotic drug used were effective in inhibiting these bacteria and fungi respectively. The effect on B. subtilis, P. aeruginosa and C. albicans were comparable to that of standard antibiotics. The inhibitory effect is very identical in magnitude and comparable with that of standard antibiotics used. Keywords: Withania somnifera, Raoultella planticola, Escherichia coli, Staphylococcus aureus P-141: CHEMOTAXONOMIC STUDY ON FEW SEED TAXA OF FAMILY SAPOTACEAE USED AS FOLKLORE MEDICINES Bindu Gopalkrishnan*, C.L. Ringmichon & Late, Shraddha N. Shimpi Mithibai College of Arts; Chauhan Institute of Science & Technology, Mumbai Family Sapotaceae includes approximately 800 species of evergreen trees throughout the world. Majority of the plants are cultivated for its flowers or edible fruit. The three taxa Madhuca latifolia (Roxb.) Macbride, Mimusops elengi Linn. and Manilkara hexandra (Roxb.) Dubard are taken for the investigation. In Western Ghats the tribal's use the seeds of the said plants for curing common ailments like stomach diorder, skin infection, piles, dental problems etc. This medicinal value may be due to some biomolecules that produce a definite physiological action on the human body. Hence the preliminary phytochemical analysis was carried out in seed, which indicated the presence of protein, tannin, glycosides like Saponins etc. among these saponins are the major secondary metabolites. Therefore the seeds are further studied for comparative phytochemical analysis using chemical marker such as "saponins". Standard methodology like HPTLC, TLC and LC-MS was used to confirm the results. Thus these folklore medicines not only have curative properties but also are of chemotaxonomic interest. Keywords: Madhuca latifolia, Mimusops elengi, Manilkara hexandra, phytochemistry P-142: EXTRACTION OF ANTIMICROBIAL METABOLITES FROM HALO-TOLERANT ALKALIPHILIC ACTINOMYCETES ISOLATED FROM THE GREAT RANN OF KUTCH Jignasha T. Thumar, Bhavin P. Pethani and Kinjal Dhulia Post Graduate Department of Microbiology, Shree M. & N. Virani Science College, Rajkot The desert environment is a habitat for many unique microorganisms, which produce biologically active compounds ("bioactives") to adapt to particular environmental conditions. Two halo-tolerant alkaliphilic actinomycetes designated as, Kut-8 and HA5 were isolated from the Great Rann of Kutch, Western India. They have been identified as Streptomyces aburaviensis and Streptomyces spp. respectivelybased on the chemotaxonomic characteristics, including cell wall constituents. The isolates produced the antimicrobial metabolites optimally at 5-10 % NaCl and pH 9-10 during stationary phase of their growth. Kut-8 produced the antimicrobial compound selectively against Bacillus cereus, while HA5 against Bacillus subtilis and Salmonella typhi. Partial purification of Kut-8 antibioticmetabolite by charcoal absorption and methanol extraction
resulted in enhanced antimicrobial activity by 4.16-fold. The crude antibiotic metabolites were separated by various solvent systems with hexanemethanolwater (Kut-8) and Butanol-acetic acid-water (HA5) giving the best separation. The results of bioautographs revealed the presence of single active compound in the Kut-8 antibiotic filtrate. However, two sharp zones against S. typhi were evident in bioautography indicating the presence of two active metabolites in HA5-antimicrobial preparation. The MIC of HA5 metabolite was 600 ug/ml for S. typhi. The study holds significance as only few halo-tolerant alkaliphilic actinomycetes from saline deserts have been explored and information on their antimicrobial potential is still scarce. It is quite evident from the above results that the secretions and properties of antimicrobial metabolites, from the taxonomically unique populations of extremophilic actinomycetes, would be very useful in recent discovery of novel secondary metabolites. Keywords: Halo-tolerant alkaliphilic actinomycetes, antimicrobial metabolites P-143: A COMPLETE REVIEW ON AN IMPORTANT MEDICINAL PLANT: ENICOSTEMMA LITTORALE BLUME Raviya Jignesh*, Patel Vishal RK College of Pharmacy, Rajkot, Gujarat Enicostemma littorale (Gentianaceae) is a glabrous perennial herb traditionally used as an antidiabetic, urinary astringent, antiperiodic, anthelmintic, anti-inflammatory, laxative and carminative. It possesses antioxidant, hypolipidemic, antimicrobial, antinociceptive, antiedematologinic and antitumor activities also, thereby covering a wide spectrum of pharmacological effects. Macromorphology and microscopy of this plant has been studied to establish its salient diagnostic features. The preliminary phytochemical analysis and thin layer chromatography has also been performed. Several recent pharmacological investigations comply with the traditional uses of the plant. A comprehensive review of this plant could be useful in setting some diagnostic indices for identification and preparation of the monograph of the plant as well as serve as a guide for further phytochemical investigations and exploration of its pharmacological and clinical aspects. P-144: IMMUNOMODULATORY ACTIYITY OF BARK OF THESPESIA POPULNEA LINN S. B. Jagadale, J. B. Kandale, M. K. Shelar Padmashri D. Y. Patil institute of pharmaceutical sciences and research, Pimpri, Pune Immunomodulatory activity of petroleum ether, ethyl acetate, alcoholic and aqueous extracts of Thespesia populnea was evaluated by using haemagglutination antibody titre, delayed type hypersensitivity reaction and cyclophosphamide induced myelosuppression in Swiss albino mice. Thespesia populnea showed effective increase in haemagglutination antibody titre, delayed type hypersensitivity reaction and was found active in normalizing WBC levels in case of cyclophosphamide induced myelosuppression in mice. Present study, therefore reveals that the drug holds promise as immunomodulatory agent, which acts by stimulating both humoral as well as cellular immunity. Keywords: Keywords: Thespesia populnea Linn., immunomodulatory activity, haemagglutination P-145: ISOLATION AND CHARACTERIZATION OF "THE KING OF BITTERS" BY DIFFERENT ANALYTICAL TECHNIQUES Priya A Shah, Rakesh K Patel, Hardik P Patel L.J. Institute of Pharmacy, Ahmedabad, Gujarat The main objective of the investigation was to optimize a suitable extraction method and to isolate and characterize the Andrographolide (diterpenoid lactone) obtained from the leaves of Andrographis paniculata (Burm. F.) Nees (Acanthaceae). It is been used as hepatoprotective, also commonly indicated for the relief of cold and flu symptoms, gastritis and upper respiratory tract infections. It is known for its potent antiinflammatory and broad-spectrum anti-microbial actions. Preliminary phytochemical screening of the various extracts of the leaves revealed the presence of flavones and terpenoids. The crude compound isolated by using methanol as an extracting solvent showed 1.1%w/w (1.1 gm) yield, identified by its melting point (227°C), TLC analysis, FTIR and RP-HPLC. Rf values of crude compound and that of standard in solvent system chloroform: methanol (7:1) under UV light at 254 nm was found to be 0.56. From the FTIR graph the compound was found to possess an asymmetric C-O stretching in lactone ring at 1250 cm-1, a free OH group attached to the lactone ring demonstrating a peak at 3340 cm-1, an asymmetric peak of Lactone at 1725 cm-1, an asymmetric peak of C-H deformation in CH2- at 1480 cm-1, an asymmetric peak of CH=CH at 1650 cm-1. The calibration curve resulted in a linear plot with regression coefficient of 0.9992. The isolated compound was demonstrating highly comparable results with the standard used as reference compound. These findings gave an idea about the purity of the isolated compound, which will be further subjected to formulate the NDDS formulations and also analyze its biological activities. Keywords: Andrographis paniculata, Isolation, Characterization P-146: MANAGEMENT OF COMMON RESPIRATORY PROBLEM IN CHILDREN A SIDDHA THERAPEUTIC APPROACH K. Suresh1, G. Ganapathy1, R. Pattarayan2, K. Manickavasakam1 1National Institute of Siddha, Chennai; 2Dean, Sivaraj Siddha Medical College, Salem Siddha medicine is an ancient system of medicine, developed by Siddhars The Man who attained perfection in life. Managing of diseases affecting children is a specific speciality of practice in Siddha system of medicine. Balavagadam (Kuzhandhai maruthuvam) is the name given to all Siddha literatures dealing with diseases of
children. Respiratory problems in children are prevalent all over the world and it plays a major role in making the school going children not to attend the school regularly. Prevalence of acute asthma in India below 15years of age groups per 100,000 populations is 19.25%. In the Out patient department of Ayodhidoss Pandithar hospital, National Institute of Siddha around 1100 patients are visiting every day for treatment for various ailments. Among them, 3 - 4% of patients are from pediatric group and are mostly affected by common respiratory problem. Management of respiratory diseases through Siddha System of Medicine is to potentiate the immune system of the individual child in order to reduce the susceptibility towards the allergens and at the same time providing complete relief from the diseases. Siddha system of medicine having more number formulations and they are not developing any resistance in the pediatric age group. So the intended explain the clear roadmap to treat common respiratory problems affecting children through Siddha medicine. Keywords: Kanam, Siddha medicine, common cold, Childhood Asthma, Bronchitis. P-147: SIDDHA APPROACH IN PAEDIATRIC CARE K. Suresh1, R. Pattarayan2, G. Ganapathy1 1National Institute of Siddha, Chennai; 2Sivaraj Siddha Medical college, Salem Siddha system is one of the ancient systems of medicine indigenous to our country. Medicine as everyone knows is not merely a science but art as well. Siddha system of medicine not only deals with physical body but also with the inner man or the soul. The word `Siddha' means "established truth". The word Siddha comes from the word 'Siddhi' that means an object to be attained such as perfection in life or heavenly bliss. Siddha system of medicine was inherited by them from Lord Siva. Siddha propounds that the physical structures of the universe and man are basically made up of the same stuff, namely five elements. They are Nilam (Earth), Neer (Water), Thee (Fire), Kaatru (Air), Aagaayam (Sky). These elements are present in different proportions in all living and non-living things. The following verse of Siddhar Sattamuni reveals this: "What exists in the world is in man" - Sattamuni gnanam. Siddha system of medicine elaborately described the classification of diseases of mankind. Paediatric care and disease management is a distinctive part mentioned in this system. This paper deals with the care for the ailments of paediatric population by siddha system of approach by exploring various siddha literatures. P-148: PHYSICO-CHEMICAL ANALYSIS OF "THIRITHODA MATHIRAI" A TRADITIONAL SIDDHA MEDICINE IN THE TREATMENT OF VARIOUS DISEASES S. Visweswaran, B. Devi, M. Murugesan, K. Manickavasakam National Institute of Siddha, Chennai Siddha system of medicine is one of the ancient system of medicine exists in this world originated in south India and practiced in tamil speaking parts of world. It deals with prevention, treatment of Diseases and also strives to prolong the longevity of human life as well as quality of life. As per Siddha system of Medicine derangement of three thodams (Vatham, Pitham and Kabam) are considered for diseases of mankind. Therapeutic aspect of Siddha medicines consists of vegetable, animal and mineral components in the treatment of various diseases. In this study a herbo mineral preparation ie, Thirithoda mathirai, is a Siddha herbo-mineral formulation, mentioned in Classical Siddha text and indicated for various diseases (especially for respiratory problem). It is one among the most commonly and widely used medicine by Siddha practitioners and Government Siddha hospitals and it is being used for many years. In this study is aimed to analyze its bio chemical and physicochemical properties using modern analytical methods like FT IR, SEM, ICP-OES techniques and biochemical analysis. Keywords: Vatham, Pitham, Kabam, FTIR, SEM, ICP-OES P-149: GASTROPROTECTIVE AND PROBABLE MECHANISTIC ACTIVITY OF THE ETHANOLIC ROOT EXTRACT OF POTENTILLA POLYPHYLLA WALL Damiki Laloo, Satyendra K Prasad, K. Sairam, S. Hemalatha Department of Pharmaceutics, Institute of Technology, Banaras Hindu University, Varanasi OBJECTIVE: To evaluate the gastroprotective effect of alcoholic root extract of Potentilla polyphylla Wall. (APP) (Rosaceae) on various experimental ulcer model. METHODS: The gastroprotective effect of the APP graded dose (100, 200 and 400 mg/kg, p.o.) were studied on three experimental ulcer models viz. 4 hour pylorus ligation (PL), 100% ethanol (1ml/ 200g, 1 hour) and 2 hour cold restrain stress induced ulceration (CRS). The ulcer index and percentage protection was calculated and the gastric juice collected from 4h PL rats was studied for the effects on gastric volume, pH, free acid and total acid output. Effects on stomach glandular weight, cell shedding and mucosal cell proliferation were also studied. RESULTS: Oral treatment of EPF (200 and 400 mg/kg, p.o.) for 5 days showed significant protection against acute gastric ulcer induced by pyloric ligation and 100 % ethanol but there was no effect found on CRS model. The gastric secretion studies showed that APP significantly increase the gastric juice pH but there was a significant reduction in the free and total gastric acid output. The extract also showed a significant effect on cell shedding and cell proliferation but no effect was found on stomach glandular weight. CONCLUSION: The findings of the present study indicate that the gastroprotective effect of APP may contribute wholly or partially due to its local effects on the gastric secretion. Further, investigation on the probable mechanistic studies (other than the gastric secretion studies) regarding the exact mechanism of action is needed to be done.
P-150: STANDARDIZATION OF POLYHERBAL FORMULATION FOR PSORIASIS Patel HV, Thakkar VY, Patel JA, Shah VN, Patel CS, Sheth NR Department of Pharmaceutical Sciences,Saurastra University, Rajkot Objective: In the present study deals with Development of Polyherbal formulation for Psoriasis and its Standardization. Formulation was Standardized by using Phytomarker by HPTLC analysis. Material and method: Evaluation of Quality control parameters for raw material like Organoleptic, Physicochemical parameters was carried out. To determine the Extractive and ash value. Preliminary phytochemical screening of Formulation where Qualitative chemical tests for identifying various phyto-constituents present were carried out on formulation powder.Quantification of Gallic acid, Curcumin and Quercetin in Polyherbal formulation by HPTLC: HPTC was carried out by using a Hemilton 100 l HPTLC syringe, Camag Linomat V automatic spotting device, Camag twin trough chamber, Camag TLC Scanner-3, WINCAT integration software, aluminium sheet precoated with Silica Gel 60F254(Merck), 0.2 mm thickness. Result: Different conc. of formulation was applied on HPTLC plate for estimation of Gallic acid, Curcumin and Quercetin. The HPTLC plates were developed in a solvent system Toluene: Ethyl acetate: Formic acid (4.5: 3: 0.2) and dried and scanned densitometrically at 366 nm. The peak area 897.16 ± 6.84 for Gallic acid, 1360.03 ± 10.15 for Curcumin and 914.56 ± 7.266 for Quercetin. Gallic acid was found to be 1.860 ± 0.014%, Curcumin was found to be 1.618 ± 0.012%, and Quercetin was found to be 0.4693 ± 0.0036% in methanolic extract of formulation. Relative standard deviation (% CV) for Gallic acid is 0.76, Curcumin is 0.74 and Quercetin is 0.78.Conclusion: A TLC densitometric method is established for the simultaneous quantification of Gallic acid, Curcumin and Quercetin from Polyherbal formulation Psoriasis using HPTLC fingerprinting. The methods were found to be simple, precise, specific, sensitive and accurate and can be used for their quantification in plant materials and also in routine quality control of the raw materials as well as formulations containing any of these compounds. Keywords: Gallic acid, Curcumin, Quercetin, HPTLC P-151: A PHARMACOGNOSTICAL STUDY ON CAPPARIS DECIDUA EDGEW. STEM P.D. Verma, R.D. Dangar, B.N. Suhagia Department of Pharmacognosy, L. M. College of Pharmacy, Ahmedabad Objective: Capparis decidua (Family, Capparidaceae), commonly known as `Kair', is a xerophytic plant distributed throughout the arid regions of India and other countries. The plant is used traditionally for various medicinal purposes. The present study is aimed at development of pharmacognostical and physico-chemical parameters of Capparis decidua stem. Materials and Methods: Fresh stems of C. decidua were collected and studied for salient diagnostic characters. Macroscopical and microscopical studies were carried out for whole and powdered drug. Loss on drying, ash values and extractive values were also determined. Results: The stem is much branched; each branch is slender, smooth and bear spines. Mature branches are leafless and show brownish bark. Microscopically, young stem shows epidermis with sunken stomata and a thick cuticle, hypodermis made up of palisade-like cells and sclereids, cortex with stone cells band and group of cortical fibres, pericyclic fibres, a ring of vascular bundles and well-developed pith with pitted and lignified cells. Numerous crystals of calcium oxalate and starch grains are found throughout the transverse section. Old stem shows a similar structure, but epidermis and hypodermis are replaced by cork and cork cambium. Powdered stem shows epidermis, stone cells, sclereids, fibres, xylem vessels, crystals of calcium oxalate, starch grains and fragments of cortex and pith. The moisture content was observed to be 44%. The extractive values in alcohol and water were found to be 1.67% and 5% respectively. Ash values such as total ash, acid insoluble ash and water soluble ash were determined as 8.13%, 0.5% and 6% respectively. Conclusion: The pharmacognostical parameters developed in the present study would serve as useful tool in standardization of Capparis deciduas plant. P-152: A CLINICAL STUDY TO COMBACT THE SIDE EFFECT OF RADIATION WITH ASWGANDHA IN ADVANCED CASE OF HEAD & NECK CANCER AND CERVICAL Rajnikant R. Sarvaiya, Bhavna G.Joshi, Gaurang Joshi Atharva Multispeciality Ayurveda Hospital,Cancer research Centre Radiotherapy is used for the treatment of local or regional disease when surgery can not completely remove the cancer. Although RT is frequently used as primary or curative mode of therapy in so many cancers but It is also well suited to palliative management of problem e.g. bony metastasis and nodal metastasis. The overall objective of this study is to compare the efficacy of radiotherapy plus herbal therapy in previously untreated oral cavity and cervical cancer. Important efficacy endpoints will be objective tumor response rate, duration of response, proportion of patient with early progressive disease or early response-stable disease.The main property of Aswagandha is a natural Anti-oxidant, which helps to increase the molecular effect of radiation. Thus this drug acted both directly on cancer cells and by making them more sensitive to Radiotherapy. Aswagandha helps to decrease the amount of Glutathione in tumor cells after which radiation therapy become more effective.These paper reviews the pharmacological treatment of concomitant therapy of cancer in a guideline format in facilitate the translation of current knowledge into clinical practice. It will also provide a model for better care of the patients who suffering from cancer. Keywords: Ayurved, Radiotherapy, Cancer, Ashwagandha P-153: ANTI-DIABETIC ACTIVITY OF AMOMUM SUBULATUM ROXB. FRUIT CONSTITUENTS Vavaiya R.B.1, Patel Amit2, Manek R. A.3
Jagdishprasad Jhabarmal Tibrewala University, Rajasthan; 2B M Shah College of Pharma. Education & Research, Modasa; 3BK Mody Govt. Pharmacy College, Rajkot Large cardamom (fruit of Amomum subulatum Roxb, Zingiberaceae) commonly known as 'Bari Ilaichi' is used in various disease condition and also described in Ayurveda. Anti-diabetic activity of Amomum subulatum Roxb. was evaluated in fructose fed metabolic syndrome in rat. Acetone and methanol extracts were assayed for total phenolic contents by UV method. Presence of protocatechuic acid was estimated by HPTLC method. Oral administration of both A. subulatum extracts revealed a significant (P<0.001) increment of serum insulin levels, higher reduction in hyperglycemia when compared to the diabetic control rats (P<0.001). The histological studies of the endocrine region of pancreas of diabetic animals revealed that shrinkage of cells of islets of langerhans. Animals treated with both extracts of A. subulatum, revealed restoration of -cells. This activity of acetone and methanolic extract might be due to presence of phenolics like protocatechuic acid. Keywords: Amomum subulatum Roxb., protocatechuic acid, HPTLC, anti-diabetic
P-154: TRADITIONAL PHYTO-THERAPIES IN LIVESTOCK ALMENTS CARE AMONG THE NATIVE INHABITANTS OF IMAMPUR GHAT AREAS IN AHMEDNAGAR DISTRICT, (M.S.) INDIA Salave A.P.1, P.G.Reddy2, P.G. Diwakar3 1Shri Dnyaneshwar Mahavidyalaya, Newasa, Ahmednagar; 2Dept.of Botany and P.G. Research Centre, P.V.P. College, Pravaranagar; 3Botanical survey of India, Western circle, Pune Present exploration was undertaken in the areas Imampur ghat located in Ahmednagar district (M.S.) India. The study was undertaken to collect information on uses of the native ethno-flora in curing certain kind of livestock ailments. In all total 7 plants species from 5 angiosperm families were studies. Of these, maximum numbers of species belong to euphorbiaceae with 3 species, acanthaceae with 2 species and fabaceae and cactaceae with 1 species each. Information gathered, indicates that the tribal, rural and aboriginal populace possess good knowledge regarding uses of the native plants in regards to treat certain livestock ailments. Majority of preparations are from leaves (3), root (2), seed (1) and fruit (1). Keywords: Livestock, Imampur Ghat, ethno-flora, Ahmednagar, ailments P-155: PHARMACOGNOSTICAL EVALUATION OF BLEPHARISPERMUM SUBSESSILE DC LEAF Amruta Jadhav, Rabinarayan Acharya, VJ Shukla, Harisha CR Institute for PG teaching and Research in Ayurveda, Gujarat Ayurved University, Jamnagar Objective: To study the pharmacognostical characters of a less explored folklore medicinal plant Rasnajhadi, used as one of the source plant of Rasna, Blepharispermum subsessile DC leaf. Method: The fresh plants of B. subsessile (Asteraceae), collected from its natural habitat, Odisa, in the month of October. The leaves of B. subsessile were used for the pharmacognostical evaluation, both macroscopic, microscopic and powder characters including test for histochemical, as per the guidelines of Ayurvedic Pharmacopoeia of India (API). Preliminary phytochemical investigation of the leaves was also carried out. Result: Leaves of the plant are alternate, entire or toothed. T.S. of leaf shows presence of upper epidermis with thick cuticle, undifferentiated palisade and spongy tissue, undifferentiated mesophyll chloroplast and oil globules and bicollateral vascular bundles, tannin containing cells. Results of histochemical tests and preliminary phytochemical tests shows presence of certain typical characters basing upon which the leaf can be easily identified. Conclusion: The obtained macro and microscopical characters including histochemical and preliminary physico chemical characters can be considered as standard for the identification of B. subsessile leaf. Keywords: Blepharispermum subsessile, Asteraceae, Pharmacognosy P-156: MANGIFERIN HERBOSOMES AS A POTENT HEPATOPROTECTIVE AGENT Arjun Singh Meena, Jain PK, Khurana N, Pounikar Y, Patil S, and Gajbhiye A Hari Singh Gour Vishwavidyalaya, Sagar, (M.P.) Mango is a rich source of biological active compound, mangiferin that is a C-glycosyl xanthone structure. In the present study, mangiferin was isolated from bark of Mangifera indica L. variety cultivated in India. Methanolic extract was used for isolation of mangiferin. Mangiferin have hepatoprotective activity due to free radical scavanging but its bioavailability is poor hence herbosomes was prepared and compare its hepatoprotective activity against conventional extract and standard drug by using Paracetamol inducing hepatotoxicity model. The result was obtained that herbosomes were more bioavaliable and therapeutically active than conventional extract & level of SGOT & SGPT was also comparable against standard drug i.e. Silymarin. Keywords: Mangiferin, C-glycosyl xanthone, mango, Mangifera indica L. P-157: ECOGEOGRAPHICAL DIVERSITY OF BACOPA MONNIERI IN RELATION TO QUANTITATIVE VARIATIONS OF BACOSIDE A Gaurav Garg, Poojadevi Sharma, Anshu Srivastava, Neeta Shrivastava National Institute of Pharmaceutical Education and Research, PERD centre,Ahmedabad Bacopa monnieri (Family: Scrophulariaceae) commonly known as Brahmi is a very important medicinal plant owing to its utility in various therapeutic functions like memory, respiratory, CNS and cardiac functions. The various compounds responsible for pharmacological effects include alkaloids, saponins and sterols. Saponin namely Bacoside A is the main memory enhancement constituent of the plant demanding a lot of commercial importance. Recently, Brahmi extract based drugs rich in Bacoside content are gaining a lot of popularity. Along with increasing demand, it is necessary to maintain the quality of the herbal drug because quality is essential for
safety and efficacy of herbal drugs. Quality of plant material suffers due to variation in production and content of active phytochemical which indirectly influences the efficacy of herbal drugs. There are many factors like latitude, longitude, temperature, rainfall, soil composition and climatic conditions which are responsible for variation in level of active phytochemicals (chemotype variation). Therefore, it is necessary to explore the natural habitat of plant species from different geographical regions in order to get superior chemotype for better quality population. The superior chemotype can be propagated further in suitable conditions for commercial purpose. In the present study, a total of 75 samples (15 from each region) were collected from five different geographical regions of North west India namely Ahmedabad, Surat, Baroda (Gujarat), Udaipur (Rajasthan) and Mohali (Punjab). Estimation of Bacoside A content was carried out using HPTLC. The different localities have been divided into high and low production regions on the basis of their Bacoside A content which indicates strong association with the ecogeographical conditions of the region of collection. Keywords: Bacopa monnieri, ecogeographical diversity P-158: CALLUS CULTURE STUDIES ON JASMINUM MALABARICUM - AN ENDEMIC MEDICINAL PLANT Pramod HJ, Harsha HV, Sandeep RP, Sanit SG KLE University's College of Pharmacy Jasminum malabaricum belonging to the family Oleaceae is endemic to Western Ghats of India. It is a climber, with white flowers and fragrance and known for its ethno medicinal importance like antibacterial, antioxidant, blood purifier, anti-tumor properties. The extensive exploitation of this species has led to reduction of its natural population. Owing to its attributes callus culture studies was carried out using Murashige and Skoog medium with different combinations and concentrations of BAP, NAA and 2,4D. The leaves and stem segments were used as explants for callus growth and leaves responded significantly to produce callus. The total phenolics present in the callus culture were estimated. Keywords: Jasminum malabaricum, callus, phenolics P-159: STUDY OF ENDOPHYTIC FUNGI ISOLATED FROM LEAVES OF HYDNOCARPUS PENTANDRA Pramod HJ, Harsha HV, Sandeep RP, Abhijit BP KLE University's College of Pharmacy Hydnocarpus pentandra Buch-Ham (Flacourtiaceae) an endangered species from Western Ghats region of India has been exploited traditionally against leprosy, rheumatic pain and inflammation. Ten endophytic fungi were isolated from plant. APEF-12,13 (Dematicious sp., Fusarium sp.) showed maximum activity against bacteria B. subtilis and E.coli and APEF-06,15(Cladosporium sp., Non sporulating hyaline form) were most active against fungi C. albicans. Keywords: Hydnocarpus pentandra, Endophytic Fungi P-160: WOUND HEALING POTENTIAL OF OCIMUM BASILICUM (LINN.) IN INCISION WOUND MODEL IN RATS Gaurav Dubey*, A.K. Pathak Department of Pharmacy, Barkatullah University, Bhopal, Madhya Pradesh A wound is break in the skin (the outer layer of the skin is called the epidermis). Wound are usually caused by cuts or scalps, symptoms at wound or injury include swelling, stiffness, tenderness, discoloration skin tightness, scabbing, itching and scar formation. The present study aims to investigate the possible wound healing effect of hydroalcoholic extracts of aerial parts of Ocimum basilicum. In the present study Incision wound model was used to evaluate the wound healing activity of Ocimum basilicum, The main objective of this investigation is to develop a product, which may give a wound healing property, and enhance wound healing process like increase the collagen synthesis, fibroblast proliferation, angiogenesis and epithelization, In the incision wound model, rats depilated by removing hairs at the dorsal thoracic region before wounding. Rats were anaesthetized by diethyl ether prior to incision. Six centimeter long paravertebral incisions were made through full thickness of skin on either side of vertebral column of the rat. The wounds were closed with interrupted sutures of one centimeter apart. The categorization and treatment of experimental animals was similar to that of excision wound model. The sutures were removed on 8th post wounding day. The tensile strength of wounds was measured on 10th day following continuous water flow technique The result showed that hydroalcoholic posses a definite prohealing action Significant increase in tensile strength was observed in incision wound. Keywords: Ocimum basilicum, Incision wound model, hydroalcoholic, ointment. Col P-161: CHEMICAL COMPOSITION OF THE ESSENTIAL OILS OF TWO SALVIA SPECIES FROM IRAN FLORA Majid Rahmanzadeh Mamaghani, Sheykhabbasi Mahdiye Department of Plant Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran The genus Salvia L. is one of the largest genera in the Lamiaceae family. The word Salvia was derived from the Greek word ``Salvere: healer, curative" and refers to the numerous medicinal applications of the plants of this genus. Salvia verticillata L. and Salvia sahendica Boiss. & Buhse are endemic plants of Iran and their aerial parts have been used as flavoring agent in dairy products and tea for more than millennia. In this investigation we analyzed chemical composition of the essential oil of some populations of S. sahendica Boiss. & Buhse. and S. Verticillata L. The aerial parts of S. sahendica Boiss. & Buhse and S. verticillata L. were collected during the flowering period from wild-growing plants, at an altitude of 1700-2450 m (summer 2009). The air-dried, powdered aerial parts (flowers and leaves) of the plant were subjected to hydro-distillation in a Clevenger-type apparatus for 3 h. The volatile oil was dried over anhydrous sodium sulfate and stored at 4 °C in the dark until
analysis. The oil was analyzed by GC/MS. Identification of components in the oil was based on retention indices and kovats indices relative to n-alkanes and computer matching with the Willey library. The chemical composition of S. sahendica Boiss. & Buhse essential oil was characterized by high content of monoterpene hydrocarbons (MH) and -Pinene as the major constituents in all populations. In contrast, the main component of S. verticillata L. essential oil was MH and oxygenated sesquiterpene with -Phellandrene (21.65-37.9 %) , Germacrene D(19.69 %), Spathulenol (12.64 %), 1,8-Cineole (11.32 %) and -Terpineol (10.86 %) as the major constituents in different populations. Keywords: Essential oils, Salvia sahendica, Salvia verticillata, GC-MS P-162: COMPARATIVE PRELIMINARY ANTI-INFLAMMATORY ACTIVITY OF SOLANUM SISYMBRIIFOLIUM (LAM.) LEAF, STEM AND ROOT Patel AV1, Vaghela JP2, Dudhrejiya AV1, Sheth NR1 1Dept. of Pharmaceutical Sciences, Saurashtra Uni.; 2S.J.Thakkar Pharmacy College, Rajkot, Gujarat The preliminary anti-inflammatory activity of Solanum sisymbriifolium (Solanaceae) methanol extract of leaf, stem and root was evaluated on carageenan induced rat hind paw oedema acute model. The leaf extract (50,100,200 mg/kg per body wt.), stem extract (100,200, and300 mg/kg per body wt.) and root extract (100, 200 and 300 mg/kg per body wt.) have been found to possess significant anti inflammatory activity on the tested experimental model. The methanol extract of leaf at dose 200 mg/kg (per body wt.), stem and root extract at same dose of 300 mg/kg (per body wt) exhibited significant anti-inflammatory potential in experimental model. In the acute experimental model the leaf extract, the stem extract and root extract showed 86.74 %, 80 .67 % and 61.66 % inhibition in carageenan induced rat paw oedema respectively. The effect produced by the extracts was comparable to that of acetylsalicylic acid, a proto type of a non-steroidal anti inflammatory agent. Keywords: Solanum sisymbriifolium, Anti-inflammatory, Carrageenan, Aspirin P-163: BIO-ACTIVITY DIRECTED ISOLATION OF INDOLE ALKALOID FROM ALSTONIA SCHOLARIS STEM BARK FOR BROAD SPECTRUM ANTI-BACTERIAL AGENT Mayurkumar B. Patel Atmiya Institute of Pharmacy, Rajkot, Gujarat Alstonia scholaris R. Br. (Apocynaceae) is widespread medicinal plant traditionally used in many countries to treat infectious disease. Phytochemical studies and antibacterial activity of stem bark of Alstonia scholaris R. Br. were investigated in present study. Various extracts of stem bark like petroleum ether, acetone, ethyl acetate, and chloroform, hydro alcoholic were prepared and evaluated for presence of phytochemicals. Extracts showed the presence of alkaloids, flavonoids, proteins, amino acids, carbohydrates, cardiac glycosides, saponins, tannins, steroids and terpenoids. Antimicrobial activity evaluated against Bacillus megaterium, Bacillus subtillis, Bacillus cereus, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Shigella sonnei. The hydro alcoholic extract and chloroform fraction of stem bark exhibited broad spectrum antimicrobial activity compare to other solvent extracts. Bioactive fraction was chomatographed and subjected to TLC Bioautgraphy for identification of the most potent compound. The most effective compound was identified (ASF-4) as indole alkaloid. It was isolated by preparative TLC. MTT assay method was used to determine IC50 values against Staphylococcus aureus, , Bacillus subtilis, Pseudomonas aeruginosa and Salmonella typhi organisms and were found to be 218.83, 235.85, 151.24 and 218.61 µg/mL respectively. Keywords: Alstonia scholaris, Indole Alkaloid, Anti-bacterial Agent P-164: IDENTIFICATION OF TERMINALIA ARJUNA ADULTERANTS USING CLASSICAL TAXONOMY AND CHEMOTAXONOMY Triveni Pardhi, Neeta Shrivastava, Sheetal Anandjiwala National Institute of Pharmaceutical Education and Research, Ahmedabad, Gujarat The genus Terminalia is well known for its medicinal properties, as described in the Indian Pharmacopoeia. The stem bark of Terminalia arjuna is used by the Ayurvedic physicians in India for the treatment of various cardiovascular diseases, collectively referred to as Hrdroga. Reports mention that about 13 different species of Terminalia itself are found as adulterants of T. arjuna. Some other plants from the same family (Combretaceae) are also found to be adulterated with Arjuna e.g. Psidium guajava and Anogeissus latifolia. The adulteration and substitution of the herbal drugs is the burning problem in herbal industry and it has caused a major treat in the research for quality control and standardization of commercial natural products.In the present study five different adulterants namely Terminalia tomentosa, Terminalia bellirica, Terminalia chebula, Terminalia catappa and Psidium guajava have been selected. These adulterants were authenticated by macroscopic and microscopic studies. Phytochemical studies were also carried out for selected plants which involved estimation of total phenolics, estimation of total tannins, development of TLC fingerprint profiles and co-chromatography of the triterpenoid rich extract and methanolic extract of T. arjuna and its adulterants. The total phenolics and total tannins ranged from 4.7-13.3%w/w and 1.3-4.1%w/w respectively, in the samples analyzed. The present study of macroscopic, microscopic and phytochemical investigation would help in delineating T. arjuna from its adulterants and can be used to improve a monograph for the proper identification of the plant. Keywords: Terminalia arjuna, Chemotaxonomy
P-165: DEVELOPMENT OF QUALITY CONTROL PARAMETERS FOR AN AYURVEDIC FORMULATIONAVIPATTIKAR CHURNA Hardik Chaudhari, Neeta Shrivastava, Sheetal Anandjiwala NIPER, PERD Centre, Ahmedabad In most of the countries herbal products are launched in market without any mandatory safety and toxicological studies. There is no effective machinery to regulate manufacturing practise and quality standard. Given the nature of products of plant origin, which by definition are never constant and are dependent on and influenced by many factors, adulterations, contaminations, misidentifications and internal quality problem due to complex phytochemicals, non uniform ingredients. Therefore, it is very essential for establishing the authencity, quality and efficacy of Ayurvedic medicines and finished herbal products.Hence the present study focuses on development of quality control parameters for Ayurvedic formulation of Avipattikar churna which is one of the most effective and widely used in hyperacidity, constipation, digestive impairments, retention of urine, metabolic disorder. It contains 14 ingredients Zingiber officinalis, Piper nigrum, P. longum, Terminalia chebula, T. belerica, Embelica officinalis, Cyperus rotundus, Embelia ribes, cardamommum, Cinnamommum tamala, Syzygium aromaticum, Ipomoea turpethum and Vida Lavana and cane sugar. Individual ingredients as well as the in-house and marketed formulations were subjected to macroscopic-microscopic studies, physiochemical analysis, and TLC analysis to confirm the identity, purity, strength of the polyherbal formulation and also to fix the quality standard of formulation. Keywords: Avipattikar churna, Standardization, TLC P-166: ANTIBACTERIAL ACTIVITY OF STEM BARK AND STEM WOOD OF NOTHAPODYTES NIMMONIANA Khan Nazeerullah, Tamboli ET, Kumar Sunil, Kumar Arvind, Roy RK K.N.Modi Institute of Pharmaceutical Education and Research, Modinagar, UP The aim of present research was focused on investigating the antibacterial and preliminary phytochemical screening of Nothapodytes nimmoniana (J.Graham) via In vitro approach. Dried stems bark and stem wood powder of Nothapodytes nimmoniana (family-Icacinaceae) were successively extracted with petroleum ether, chloroform and methanol using Soxhlet and macerated to form water extracts. All extracts were screened for its antibacterial activity using agar well diffusion method. The microorganisms used for antibacterial activity were Bacillus subtulis, Staphylococcus aureus, Escherichia coli, Aeromonas hydrophilla and Salmonella typhii. Streptomycin was used as standards. The extracts showed antibacterial activity were subjected to minimum inhibitory concentration assay by broth dilution method. Petroleum ether, chloroform, methanol and aqueous extract exhibited in-vitro antibacterial activity. Among these, methanol and aqueous extracts were found to have a more potent inhibitory effect comparing with the other extracts. Keywords: Nothapodytes nimmoniana, antibacterial activity P-167: ANTI-ALZHEIMER ACTIVITY OF CENTELLA ASIATICA - MODERN SCIENTIFIC EVALUATION Rahul Dutt Karra , N R Sheth , Dhanabal P , Duraiswamy B , Nanjan M J Department of pharmaceutical Sciences , Saurashtra University , Rajkot Alzheimer's disease, the most common form of dementia, is a progressive disorder characterized by widespread loss of brain cells called neurons, beta-amyloid deposits in the cerebral blood vessels, development of plaques and the presence of neurofibrillary tangles. These changes, occurring in the association area of the cerebral cortex, the hippocampus and the middle and temporal lobes, are accompanied by decreased concentrations of the neurotransmitter acetylcholine. Currently there are no medicines that can slow the progression of AD. However, three FDA-approved medications are used to treat AD symptoms.. However, they will not stop or reverse AD and appear to help individuals for only a few months to a few years. Donepezil (Aricept), rivastigmine (Exelon), and galantamine (Razadyne) are prescribed to treat mild to moderate AD symptoms. Certain herbs namely Ginkgo Biloba, Salvia Officinalis , Muira puama , Bacopa monniera , Rosmarinus officinalis , Turmeric ,German chamomile ,Panax ginseng ,Licorice ,White willow bark ,Ginger ,Chinese knotweed or Fo-Ti ,Stinging nettle ,Maca ,Maritime pine bark are being prescribed for the management of dementia including Alzheimer's disease . In recent research Centella asiatica was found to be effective in preventing cognitive deficits in an intracerebroventricular STZ model of alzheimer's disease in rats. Phytochemical and pharmacological scientific validation of C. asiatica will be discussed in detail in the full paper. Keywords: Alzheimer, dementia, Centella asiatica P-168: ESTIMATION OF STIGMASTEROL AND SALICYLIC ACID IN LEPTADENIA RETICULATA (JIVANTI) AND ITS SUBSTITUTES Raval M A, Vyas N V, Mishra S H Ramanbhai Patel College of Pharmacy, Changa. Dist. Anand, Gujarat Leptadenia reticulata is one of the ingredients in many lactogenic herbal/traditional formulations for internal use and also in few of cream based formulations meant for topical application. Dregea volubilis and Pentatropis microphylla are common substitutes for L. reticulata a potential lactogenic plant used traditionally. The drugs lack markers to assess the quality of raw materials and extracts. The study revealed the presence of salicylic acid in plant extracts. A method using HPTLC was developed to estimate stigmasterol and salicylic acid from various organs of these drugs. The validated method is found suitable for estimation of stigmasterol and salicylic acid in methanolic extract of the selected plants. Maximum amount of salicylic acid was present in the
roots of P. microphylla while stigmasterol was present in the highest amount in aerial parts of L. reticulata, amongst the plants analyzed. Keywords: Leptadenia reticulata, Dregea volubilis, Pentatropis microphylla P-169: PHARMACOGNOSTICAL STUDIES OF HYMENODICTYON ORIXENCE (ROXB.) STEM BARK Hemang N Joshi, Jignesh Kevalia, K Nishteshwar, PP Sharma Institute for Post graduation Teaching & Research in Ayurveda, Gujarat Ayurved Uni., Jamnagar Hymenodictyon orixence (Roxb.) is a medicinally important plant belongs to family Rubiaceae occurs in India, Pakistan, and Malaysia. The present pharmacognostical study includes morphological and anatomical studies for the stem bark of the plant. The bark with straight, channeled, glossy, shiny marked with horizontally running lenticels and patches of orange to reddish coloured pigments at places; microscopically stem bark shows stratified cork; various sizes and shapes of stone cells, pigment cells, prismatic crystals of calcium oxalate. Keywords: Rubiaceae, Bhramrchhallika, Pharmacognosy P-170: EVALUATION OF CERTAIN HERBAL REMEDIES SELECTED FROM LIBYAN MARKET Rajab A M Atraiky, MR Haman School of Pharmacy, Tripoli University, Tripoli, Libya Nowadays people prefer herbal remedies as they are regarded safer, low cost, their belief that certain diseases could be treated with herbal remedies where conventional therapies prove to be inadequate. It's deemed necessary to draw attention to the scarcity of sufficient data for many herbal remedies those fail to achieve satisfactory quality levels; by investigation of quality control & marketing regulatory aspects of five selected herbal remedies sold in Libyan market; they where coded as ( A, B, C, D & E). Home prepared samples (HPS) been prepared by collecting same ingredients as marketed samples in equal proportions as no strength on the label of those studied samples, and this used for microscopical and phytochemical investigations.Results revealed the ingredient names on the label in local names, difference between primary and secondary package written information, detection of foreign matters, high total count of bacteria and radioactive contamination. Keywords: Herbal remedies, contamination safety for personal use P-171: IDENTIFICATION OF SOME PHYLLANTHUS SPECIES USING CLASSICAL TAXONOMY AND CHEMOTAXONOMY Rakhi Khabiya, Anshu Srivastava, Sheetal Anandjiwala National Institute of Pharmaceutical Education and Research, PERD Centre, Ahmedabad The deliberate or undeliberate adulteration of Phyllanthus amarus with P. fraternus and P. urinaria using "Classical and Chemotaxonomy" is highlighted. In Gujarat, 11 species of Phyllanthus have been identified and among them P. amarus has been extensively used in Indegenious Systems of Medicine. Traditionally it is used for hepatoprotective, antitumour, antidiabetic, antihypertensive, analgesic, anti-inflammatory and antimicrobial properties. It is highly valued in the treatment of various liver ailments. Some of the major chemical constituents of P. amarus include phyllanthin, niranthin, hypophyllanthin, hydrolyzable tannins, phyllanthusiin D, amariin, amarulone, amarinic acid, gallic acid, ellagic acid and phyllnirurin. The plant as well as the compounds has been reported to possess significant biological activities, including inhibition of HIV replication, bactericidal, free radical scavenging, cytotoxic, gastric protective and antiinflammatory activities. This medicinally important herb is often adulterated with its allied species due to referring them with a common vernacular name, their similarity in gross morphology, close proximity in growth, habit, habitat and lack of legal guidelines to check the authenticity and quality of the medicinal plant sold. This deliberate or undeliberate adulteration of P. amarus with its allied species has been checked by using classical (study of macroscopic and microscopic characters) and chemotaxonomy (development of chemoprofile).In the present work, morphological and anatomical parameters of P. amarus, P. fraternus and P. urinaria were assessed and a simple key to differentiate them is developed. Fingerprint profile and co-chromatography with marker compounds viz. phyllanthin, hypophyllanthin and niranthin; gallic acid and ellagic acid in these species has been developed. Keywords: Phyllanthus , chemotaxonomy P-172: PHYTOCHEMICAL AND PHARMACOLOGICAL EVALUATION OF SOLANUM MELONGENA LEAVES RN Kale1, RY Patil2 1S.V.P.M'S College of Pharmacy, Malegaon; 2P.D.E.A's S.U. College of Pharmaceutical science and Research, Kharadi, Pune Solanum melongena L. is an important solanaceous crop of sub-tropics and tropics. It is an erect or suffrutescent, herbaceous, armed or unarmed, perennial plant. The leaf pattern is mostly opposite, large, single lobed and the underside of the most cultivars is covered with dense wool like hairs. The leaves may be with or without spines at the midrib portions. The leaf blade and tip angle is very acute to very obtuse. Inflorescence is often solitary but sometimes it constitutes a cluster of 2 - 5 flowers. In the present investigation dried powdered leaves of Solanum melongena were subjected to hot soxhlet extraction with water, methanol, chloroform and petroleum ether. Extracts so obtained were concentrated to dryness by evaporating the solvent. Yields were calculated. Phytochemical screenings of the prepared extracts were separately carried out. Result of the phytochemical screening showed the presence of alkaloids, carbohydrate, glycosides, Fixed oil, tannins & phenolic compounds, steroids, flavonoids and proteins & amino acids. It was concluded that as leaves of
Solanum melongena contains various important phytochemical constituents, it may be useful for treatment of various diseases. Aqueous extract was screened for antimicrobial activity using paper disc method. Staphylococcus aureus was used as test organism and activity was compared with standard drug streptomycin. The zone of inhibition of aqueous extract was found to be comparable to the standard drug. Keywords: Solanum melongena, phytochemical, antimicrobial P-173: PHARMACOGNOSTICAL STUDIES AND HPTLC FINGERPRINT OF AN AYURVEDIC POLYHERBAL FORMULATION-SITOPALADI CHURNA Avinash Patil1, Khatija Dalwai1, Swapneel Koli1, Darshana Patil2 1Dept of Botany-Herbal Sciences, Birla College, Kalyan; 2Dept of Botany, Smt. C.H.M. College, UNR-3 Standardization of herbal formulation is essential in order to assess the quality of raw material and the finished product of the drug. Sitopladi Churna (SC) is a well-known ancient traditional Ayurvedic polyherbal formulation which is official in Ayurvedic Formulary of India, traditionally used widely for respiratory tract disorders. Though SC is an age old formulation, there are very few references available on its quality control and standardisation. In the present study an attempt has been made to standardise SC (in-house preparation and two marketed formulations) based on pharmacognostical studies, physicochemical evaluation and phytochemical analysis. Piperine isa biologically activemarker compound; therefore an attempt has been made to develop HPTLC fingerprint w.r.t. Piperine, which is a value addition to the current work. With the growing demand of the herbal drugs in medicinal market, it is suggested that the results obtained from the present work will help in maintaining the quality and batch to batch consistency of Ayurvedic formulation Sitopladi Churna. Keywords: Sitopladi Churna, Pharmacognostical standardisation, HPTLC fingerprint, Piperine. P-174: EFFECT OF ETHANOLIC EXTRACT OF PAEDERIA FOETIDA LINN. LEAVES ON SEXUAL BEHAVIOR AND SPERMATOGENESIS OF MALE RATS Devendra K Soni, Vikas Sharma, V Soni, VK Dixit Department of Pharmaceutical Sciences, H. S. Gour University, Sagar Paederia foetida Linn. (Family- Rubiaceae) is considered as an aphrodisiac, but its ability to stimulate sexual behavior has not been reported. In the present study, the effects of ethanolic extract of P. foetida on sexual behavior and testosterone level in male rats were investigated. Ethanolic extract of the leaves (50,100 and 200 mg/kg body weight) were studied for their effect on body and secondary sexual organ weight, sexual behavior, spermatogenesis and serum testosterone level in male albino rats. Oral administration of extract in albino rats showed pronounced anabolic and spermatogenic effect in animals of respective treated groups. Extract significantly increased the mount frequency and intromission frequency. In addition, they also significantly reduced the mount latency and intromission latency. These effects were associated with an increase in orientation activity. The extract had a dose dependent influence on serum testosterone level. The results support the use of P. foetida as an aphrodisiac in traditional medicine. Keywords: Paederia foetida, Sexual behavior, Aphrodisiac, Penile erection, Testo P-175: CAMPTOTHECIN PRODUCTION BY NOTHAPODYTES NIMMONIANA IN-VITRO CELL CULTURES Vijai Singh Karwasara, Vinod Kumar Dixit Department of Pharmaceutical Sciences, Hari Singh Gour University, Sagar, Madhya Pradesh Camptothecin (CPT), a topoisomerase-I inhibitor, chemically a modified monoterpene indole alkaloid. Camptothecin occupies an important position among the plant-derived anti-cancer drugs. Primarily it was reported in Chinese tree Camptotheca acuminata. It is also reported in Nothapodytes nimmoniana (Icacinaceae), some species of the genus Ophiorrhiza, Ervatamia heyneana and Merrilliodendron megacarpum. Camptothecin and its analogues are clinically approved against a variety of cancers. N. nimmoniana is native to the Western Ghats of India and a rich source of camptothecin and 9-methoxycamptothecin. In order to protect the natural germplasm, a need was felt to look for alternate sources for this class of natural anticancer products. However, due to futile chemical synthesis, the commercial demand of CPT is being fulfilled by natural N. nimmoniana, which led to rapid decline in natural plant material. Plant cell cultures remain a favoured methodology for biotechnological production of naturebased compounds. In the present study, the effect of culture medium nutrients on growth and alkaloid production by plant cell culture of N. nimmoniana was studied with a view to increase the production of the alkaloid camptothecin. MS media supplemented with 10.74 µM naphthalene acetic acid and 2.32 µM kinetin showed highest callogenesis response. One month old friable callus cultures were used to initiate suspension cultures. Quantitative estimation of camptothecin was done by HPLC. The effects of carbon, nitrogen, phosphate, on cell growth and production of the CPT by cell cultures of N. nimmoniana were investigated. Amongst the various sugars tried such as glucose, fructose, maltose and sucrose, maximum accumulation of camptothecin was observed with sucrose. Higher nitrate source concentration in the media supports the biomass while higher ammonium source concentration enhances the camptothecin content. Sucrose feeding and nitrogen source feeding strategy remarkably stimulated cell biomass growth and CPT production. Finally, the selective medium has 1.7-fold higher CPT content over the control culture. Keywords: Camptothecin, plant tissue culture, nothapodytes, callus P-176: EVALUATION FOR THE ANTI UROLITHIATIC ACTIVITY OF LUANAEA PROCUMBENS AGAINST ETHYLENE GLYCOL INDUCED RENAL CALCULI IN RATS
Atul Makasana*, Dishant Desai, Vivek Parekh, Jaymin Mendpara, Vishvas Ranpariya Department of Biochemistry, Saurashtra University, Rajkot This study investigated the evaluation for the antiurolithiatic activity of Luanaea procumbens Linn. against ethylene glycol induced renal calculi in albino wistar rats. Ethylene glycol feeding resulted in to hyper oxaluria, hypercalciuria, as well as increased renal excretion of phosphate. Supplimentation with methanolic extract of leaves of Launaea procumbens (MELP) significantly prevented these changes dose dependently. The increased calcium and oxalate level and number of calcium oxalate crystal in the tissue of calculogenic rats reverted by supplementation with MELP. The treatment of MELP also prevents the impairment of renal functions. Result: indicate that the treatment of MELP can prevent ethylene glycol induced urolithiasis in rats as it reduced the growth of urinary stones. Therefore MELP is helpful in recurrence of deases. The mechanism underlying this effect is mediated possibly through nephroprotection and its effect on urinary concentration of stone forming constituents. Keywords: Luanaea procumbens Linn, Urolithiasis, Ethylene glycol, Nephrolithiasis P-177: IN VITRO & IN VIVO FREE RADICAL SCAVENGING ACTIVITY OF AMARANTHUS VIRIDIS L. Nisha Sharma1, Prakash Chandra gupta1, C V Rao2 1University Institute of Pharmacy, C.S.J.M. University, Kalyanpur, Kanpur; 2NBRI Amaranthus viridis is commonly known as chaulai in Hindi. The traditional uses reported are as diuretic, analgesic, anti-pyretic, vermifuge, anti-ulcer, hypoglycemia, asthma, laxative etc. The main objective of the study was to investigate the antioxidant potency of different fractions of Amaranthus viridis whole herb by employing various established in-vitro systems, such as -carotene bleaching, reducing power, DPPH/superoxide/hydroxyl radical scavenging, ferrous ion chelation and lipid peroxidation. On the basis of invitro antioxidant properties polyphenolic fraction of the herb AVEF was selected as the potent fraction for in vivo antioxidant property. AVEF found to inhibit the toxicity produced by CCl4 administration as seen from the decreased lipid peroxides and increased glutathione levels. Among the antioxidant enzymes superoxide dismutase and catalase levels were retored to almost normal levels compared to CCl4 intoxicated rats. The HPLC analysis indicated the presence of phenolic acids (gallic acid) and flavonoids (quercetin and rutin). Thus it may be concluded that the AVEF possess high phenolic content and potent antioxidant properties which may be mediated through direct trapping of free radicals and also through metal chelation. Keywords: Amaranthus viridis, antioxidant, phenolic acids, flavonoids P-178: ANTIDIABETIC AND IN VIVO ANTIOXIDANT ACTIVITY OF METHANOL EXTRACTS OF AGNOSMA CYMOSA ROXB. IN STREPTOZOTOCIN INDUCED DIABETIC MODEL J Sangeetha, K Abbulu, M Sudhakar Malla Reddy Institute of Pharmaceutical sciences Aganosma cymosa R. (AC) is an evergreen shrub found throughout in India. The folklore claims that the plant possesses several therapeutic properties like in the treatment of diabetes, as an antidote for snake poison etc. The present study involves the screening of antidiabetic and antioxidant property of the methanol extract of (AC) in streptozotocin induced diabetic model. The methanol extract of the whole plant of (AC) was tested at the dose of 200mg/kg and 400mg/kg in normal and streptozotocin induced diabetic rats. Further, along with Blood Glucose level the changes in body weight, serum cholesterol, triglyceride, total protein and the effect on lipid peroxidation(LPO), glutathione(GSH), superoxide dismutase(SOD) and catalase(CAT) were assessed in extract treated diabetic rats and compared with positive and normal control groups. Histopathological study of the pancreas and liver was also evaluated. The methanol extract of AC at 400mg/kg (P<0.001) and 200mg/kg (P<0.01) produced a significant reduction in fasting blood glucose level when compared with the standard and also there was a significant difference in serum lipid profiles, total protein, LPO, GSH, CAT and SOD in the extract treated group of animals. The histopathological study showed a comparable regeneration of beta cells. Hence the plant Agnosma cymosa R exhibited not only significant antidiabetic potential but is showed good antioxidant activity in streptozotocin induced diabetic rats with improved biochemical parameters. Keywords: Agnosma cymosa R, Antidiabetic activity, Antioxidant activity P-179: DEVELOPMENT OF FINGER PRINTING METHODS OF AN AYURVEDIC FORMULATION PANCASAMA CHURNA: A SPECTROPHOTOMETRIC APPROACH Gupta Vishvnath and Jain UK* Bhopal Institute of Technology & Science-Pharmacy, Bhopal (M.P) Pancasama churna (PSC) is well known ayurvedic formulation is official in Ayurvedic Formulary of India, traditionally used for digestive impairment, flatulence, abdominal pain, assimilatory disorder, rheumatic arthritis, piles and other abdominal disorders.In present study a spectrophotometric method in ultraviolet region has been developed for the determination of piperine in market and laboratory ayurvedic formulation of Pancasama churna. Three batches of pancasama churna (PSC) were prepared according to the method given in the ayurvedic formulary of India and another set of three different formulations were procured from the market. In present study a spectrophotometric method was devloped for the estimation of piperine in laboratory and marketed preprations. The concentration of piperine present in raw material of PSC was found to be 1.45±0.014 w/w in piper longum fruits. The content of piperine in different laboratory preprations were found PSC-I (0.20±0.002), PSC-II(0.22±0.006), PSC-III(0.23±0.004)w/w respetively and for marketed formulations were found to be PSC-A (0.24±0.005), PSC-B(0.27±0.007), PSC-C(0.26±0.001)w/w respetively. In
order to maintain precision and accuracy the recovery study was performed and result obtained with mean value 99.50±0.13, which prove reproducibility of the result. The mean of %RSD value was found to be 0.390 with the mean standard error 0.28. A result of statistical analysis show present spectrophotometric method for determination of piperine is simple, precise, accurate and suitable for routine analysis of piperine in pancasama churna. The devloped fingerprints can be used as a standard and piperine can be used as a possible marker compound for fingerprinting of the formulation. Keywords: Piperine, ayurveydic formulation, UV spectroscopy P-180: ISOLATION OF ALOCASIA INDICA LINN. STARCH AND ITS PERFORMANCE AS A DISINTEGRATING AGENT Nemade CT, Lodha GK Department of Pharmacognosy, SSDJ College of Pharmacy, Nashik Starch obtained from Alocasia indica Linn. tubers (Araceae) was a fine, almost white powder. The isolated starch was evaluated as a disintegrating agent by preparing a placebo tablet formulation at concentrations of 6.0 to 12.0 % w/w in the present study. The limit tests, loss on drying, and ash value were well within the official limits. The starch was also evaluated for various parameters as per Indian Pharmacopoeia. The granules prepared by wet granulation technique were evaluated for bulk and tapped densities and flow properties. Tablet properties including thickness, average weight and weight variation, hardness, friability, and disintegration time were evaluated. The disintegrant efficiency of extracted starch was compared with that of the maize starch in tablets prepared using magnesium stearate, Lactose and Guar gum as lubricant, diluent and binder respectively. The disintegration time for tablet formulations prepared using 10 % w/w extracted starch was less (149 s) than that of the tablet formulations prepared using maize starch as a disintegrant (169 s).Studies indicates that Alocasia indica Linn. Starch possesses a disintegrating property and could be useful in Conventional tablet formulation. Keywords: Alocasia indica, maize, starch, tablet, disintegrant P-181: FRUIT PEELS AS A NOVEL SOURCE OF ANTIOXIDANT AND ANTIMICROBIAL AGENTS Kalpna Rakholiya, Sumitra Chanda Dept. of Bioscience, Saurashtra Uni., Rajkot Degenerative diseases and infections are leading cause of death worldwide due to oxidative stress and multidrug resistant strains of bacteria, reduced susceptibility to antimicrobics and increase in untreatable bacterial infections. Peels are thrown into the environment as agro waste which can be utilized as a new source of antioxidants and antimicrobics. These are novel, natural sources of antioxidants and antimicrobics, which can be used in the prevention of diseases caused by free radicals and pathogenic microbes. In this work, peels of Citrullus lanatus (Thunb.) Matsumara & Nakai (Cucurbitaceae) Pithecellobium dulce Benth. (Leguminosae) and Musa paradisiaca L. (Musaceae) were investigated. Extraction was done by cold percolation method with different organic solvents (hexane, ethyl acetate, acetone, methanol and water). Total phenol and flavonoid contents were measured by Folin-Ciocalteu's reagent method and aluminium chloride colorimetric method respectively. Antioxidant activity was measured by DPPH free radical, superoxide anion radical and ferricreducing antioxidant power was estimated. In vitro antimicrobial activity was tested against ten bacterial strains and four fungi by agar well diffusion method. Amongst the different fruit peels studied, C. lanatus showed remarkable antimicrobial activity. All fruit peels showed different level of antioxidant activity. This study definitely opens scope for future utilization of the waste products for therapeutic purpose. Keywords: Fruit peels , antioxidant, antimicrobial, DPPH, SO P-182: RADICAL SCAVENGING AND ANTIOXIDANT ACTIVITIES OF PSIDIUM GUAJAVA L. (MYRTACEAE) LEAVES: A MEDICINAL PLANT FROM INDIA Mital Kaneria, Sumitra Chanda Dept. of Bioscience, Saurashtra Uni., Rajkot Psidium guajava L. (Jamphal), belongs to the family Myrtaceae. The leaves are used for the treatment of diarrhoea, leukorrhea, cholera, external ulcers, skin diseases and is claimed to be effective in many other diseases. In the present work, leaves of P. guajava were evaluated for its antioxidant property by assaying various antioxidant assays like by DPPH free radical, hydroxyl radical and superoxide anion radical scavenging assays and reducing capacity assessment. Total phenol content was measured by Folin-Ciocalteu reagent method. Extraction was done sequentially in soxhlet apparatus in petroleum ether, toluene, ethyl acetate, acetone, water, solvents of increasing polarity. A direct correlation between phenolic content and antioxidant activity was envisaged. The acetone extract showed better DPPH free radical (IC50 = 10.7 µg/ml) and superoxide anion radical scavenging activity (IC50 = 66 µg/ml) than the standard ascorbic acid (IC50 = 11.4 µg/ml) and gallic acid (IC50 = 185 µg/ml) respectively. The results revealed that leaves of this plant have antioxidant potential. On the basis of these findings, the acetone extract of P. guajava may be considered as potential therapeutic candidate for pathological conditions characterized by free radical overproduction. Keywords: Psidium guajava L., Folin-Ciocalteu reagent, DPPH, Radical scavenging P-183: EFFECT OF CISSUS QUADRANGULARIS L. ON BIOCHEMICAL CHANGES INDUCED BY CARBON TETRACHLORIDE (CCL4) IN WISTAR ALBINO RATS Krunal Nagani, Sumitra Chanda
Dept. of Bioscience, Saurashtra Uni., Rajkot The stems of Cissus quadrangularis L. (Vitaceae) are reported to have great medicinal value. The methanol extract of Cissus quadrangularis L. stem was separated into two fractions (Fraction I and Fraction II) by solventsolvent partition method. The present study describes antioxidant activity of Fraction-II of methanolic extract of Cissus quadrangularis L. stem against carbon tetrachloride (CCl4) induced stress in Wistar albino rats. Stress was induced by injecting 2 ml/kg, (i.p.) of CCl4 in equal proportion with olive oil. Treatment rats were dosed by oral gavage with 5 % Tween-80 suspension of Fraction-II of methanolic extract of Cissus quadrangularis L. with dosages of 150 and 300 mg/kg b.w. for 5 days. Vitamin-E was used as standard at a dose of 50 mg/kg b.w. Total protein, total albumin, GPT, GOT, ALP and urea were estimated from serum. The total protein and total albumin content in serum significantly decreased in CCl4 treated rats as compared to normal group. The GPT and GOT levels decreased in treated group, as compared to toxin control group. These results suggest that Fraction-II of methanolic extract of Cissus quadrangularis L. has a potent hepatoprotective effect on CCl4-induced stress in rats through its antioxidant property. Keywords: Cissus quadrangularis L., solvent-solvent partition method P-184: NUTRACEUTICAL BOTTLE GOURD AS A POTENTIAL SOURCE OF NATURAL ANTIMICROBIAL AGENT Tejas Rathod, Kalpna Rakholiya, Sumitra Chanda Dept. of Bioscience, Saurashtra Uni., Rajkot Lagenaria siceraria (Molina) standley (Cucurbitaceae) is an annual herbaceous climbing plant with a long history of traditional medicinal uses in many countries, especially in tropical and subtropical regions. Infectious diseases have continued to ravage most developing nations of the world. Nearly half of all deaths in developing nations are attributed to microbial infections. The present study was undertaken to evaluate in-vitro antimicrobial activity of hexane, methanol (100%, 75%, 50%, 25%), and aqueous extracts from different parts of bottle gourd. In vitro antimicrobial activity of all the extracts was done by agar well diffusion assay against three Gram positive, three Gram negative and three fungi. The Gram negative bacteria were more susceptible than Gram positive bacteria. Demonstration of antimicrobial activity of this plant provides the scientific basis for the use of this plant in the traditional treatment of diseases and may help to discover new chemical classes of antibiotic substances that could serve as selective agents for infectious disease chemotherapy and control. This investigation has opened up the possibility of the use of this plant in drug development for human consumption possibly for the treatment of various infections caused by microbes. Keywords: Nutraceutical, bottle gourd, antimicrobial agent P-185: STABILIZATION OF HEPATITIS -B SURFACE ANTIGEN (HBSAG) AT WATER IN OIL INTERFACE FOR ENCAPSULATION IN POLYMERIC MICROSPHERES Vivek Shrivastava and U. K. Jain Bhopal Institute of Technology and Science - Pharmacy, Bhopal, India The occurence of many infectious disesases have reduced considerable during last few dcades as a result of vacccination. Vaccination often requires multiple immunizations. Thus development of single dose vaccine have become an area of inteest. Microspheres have been evaluated for generation of single dose vaccines. Despite encouraging results, concerns about stability of antigens in microspheres have hampered the rapid progress of polymeric delivery systems. The first emulsification step during microencapsulation is considered a primary cause for protein denaturation and aggregation. The aim of work was to investigate the capabilities of natural surfactant from herbal origin to stabilize HBsAg antigen at W/O interface. The study revelaed that surfactant of herbal origin were able to prevent interface induceed aggregation of antigen. Keywords: w/o emulsion, microencapsulation, surfactant, stabilization P-186: AMELIORATIVE EFFECT OF BUTEA FRONDOSA (LEAVES) EXTRACT AGAINST SCOPOLAMINE INDUCED AMNESIA Kavita Munjal, Munish Kumar, Rahul Deshmukh, Jai Malik Department of Pharmacognosy, ISF College of Pharmacy, Moga, Punjab Objective: Butea frondosa (Fabaceae) is a moderate sized deciduous tree, widely distributed throughout India and other East-Asian countries, popularly known as `dhak', 'palash' or `flame of forest'. It is used in Ayurveda, Unani & Homeopathic medicine for its multifarious activities. Leaves have been used in some tribal areas for various CNS disorders. The present investigation was designed to evaluate ameliorative effect of B. frondosa leaves on scopolamine induced amnesia. Methods: Memory enhancing activity of lyophilized hydroalcoholic extract of B. frondosa leaves (LHAE) was evaluated against scopolamine induced amnesia using Morris water maze and object recognition models at three oral dose levels of 100, 200 and 400 mg/kg in rats. Effect on various biochemical parameters viz., acetylcholinesterase activity, malondialdehyde and glutathione levels was also evaluated in the brain of animals. Results: LHAE showed a significant (P< 0.05) and dose dependent memory enhancing activity at 200, 400 mg/kg in both the behavioral models against scopolamine induced amnesia. It significantly decreased the transfer latency and increased the time spent in the target quadrant during Morris water maze test. It also increased the ability of the animals to discriminate between the familiar and novel object in object recognition test. The LHAE (200 and 400 mg/kg) also exhibited a significant acetylcholinesterase inhibitory activity. The extracts decreased the lipid peroxidation in brain as evident from the decreased levels of MDA and increased levels of GSH. Conclusion: The results of the present study clearly
indicate the memory enhancing effect of the LHAE against scopolamine induced amnesia. Since cholinergic system and oxidative stress play an important role in controlling memory functions, it was hypothesized that the effect of the LHAE could be due to the acetylcholinesterase inhibitory and antioxidant activity. Keywords: Memory, Scopolamine, Palash P-187: EVALUATION FOR ANTI-INFLAMMATORY ACTIVITY OF ETHYL ACETATE FRACTION OF TEPHROSIA PURPUREA Rajal shah, Sumitra Chanda Department of Bioscience, Saurashtra University, Rajkot Inflammation is the complex biological response of vascular tissues to harmful stimuli, such as pathogens, damaged cells or irritants. In the present study, we evaluated the ethyl acetate fraction of Tephrosia purpurea root, (Fabaceae), for anti-inflammatory activity against histamine-induced paw edema in rats. Animals were divided into four groups; Group-I received normal saline (control), Group-II received Diclofenac sodium (standard), Group-III and IV received 25 and 50 mg/kg of ethyl acetate fraction of ethanol extract respectively. An inflammation was induced by subplantar injection of 0.05 ml of 1% histamine. Paw volume was measured in all treatment groups at 0 hr, 1/2 hr, 1 hr & 2 hr and mean paw volume was calculated. The extract showed potent anti-inflammatory activity by significantly reducing the paw volume (P<0.01) as compared to control group. P-188: COMPARATIVE STUDY OF HERBAL TERPENES AS PERMEATION ENHANCERS IN TRANSDERMAL DRUG DELIVERY OF GRANISETRON HCL. Dhaval N Lunagariya, CK Gadhiya, AV Dudhrejiya, MR Dabhi Department of Pharmaceutical sciences, Saurashtra university, Rajkot Various chemical permeation enhancers are available, but they are irritant and carcinogenic. Therefore, they are not widely used. While, herbal permeation enhancers like terpenes are regarded as safe, without any toxic effect. So, interest is given to the use of herbal terpenes as permeation enhancers for transdermal delivery of Granisetron HCl. The transdermal patches of Granisetron HCl using herbal terpenes as permeation enhancers obtained by solvent casting method. In which HPMC E 50 LV and Eudragit RS100 selected as film forming polymers and PEG400 and Propylene Glycol (8:2) as plasticizers. Menthol, Thymol and d-Limonene used as herbal permeation enhancers. All batches optimized on effect of independent variables, such as thickness uniformity, weight variation, folding endurance, % moisture uptake and loss, drug content uniformity, tensile strength, % elongation and Ex-vivo diffusion study. From the results of diffusion study through rat excised skin in 24 hour, permeation enhancement effect of terpenes showed markedly increase with increase in concentration of terpenes up to some extent, after which there were no remarkable improvement in the rate of diffusion observed. These concentrations of terpenes taken as optimized concentration for permeation enhancement. From result of three terpenes, Order of terpenes with their maximum permeation enhancement effect was: d-Limonene > Menthol > Thymol. Pure drug, patch without drug, patch with drug, patch with drug and 6% w/w strength of Menthol, Thymol and d-Limonene respectively subjected to analytical techniques like FT-IR and DSC to evaluate the absence of interaction between polymers and drug. Keywords: Transdermal patches, Granisetron HCl, Menthol, Thymol, d-Limonene P-189: PHARMACOGNOSTIC EVALUATION OF BERGIA SUFFRUTICOSA (DELILE) FENZL WHOLE PLANT, AN HITHERTO UNEXPLORED PLANT Sandeep Bind, Manish Nivsarkar, Sheetal Anandjiwala Department of Natural Products, NIPER, Ahmedabad Bergia suffruticosa (Delile) Fenzl (syn. B. odorata Edgew) (Elatinaceae family) is an important Indian medicinal plant, hitherto unexplored for pharmacognostic and phytochemical evaluation. It is used traditionally to repair bones and is applied as a poultice on sores. Ethnomedically, it is used for stomach troubles and as an antidote to scorpion stings. Ethanolic extract of the plant was reported to show antibacterial, molluscicidal and free radical scavenging activity. However, pharmacognostical and phytochemical studies of the species is lacking. With this backdrop, it becomes extremely important to make an effort towards standardization of B. suffruticosa. The present work involves the detailed macroscopic and microscopic studies of the whole plant of B. suffruticosa including the following parts: Root, stolon, stem, leaf and flower. Further, the powder microscopy of the whole plant was also carried out. It revealed the presence of rossets and clusters of calcium oxalate, numerous simple starch grains, covering and glandular tricomes, vessel elements and fibres. Preliminary phytochemical analysis revealed the presence of phytosterol, alkaloids, flavonoids and phenolics. TLC fingerprint profile and co-chromatography was carried out with phenolics viz. gallic acid and gallicin using toluene: ethyl acetate : methanol : formic acid (6 : 3 : 1 : 0.5, v/v) as the mobile phase and they resolved at sitosterol andRF 0.40 and RF 0.52 respectively; and with phytosterols viz. -Sitosterollupeol using toluene: methanol (9 : 1 v/v) as the mobile phase. and lupeol resolved at RF 0.41 and RF 0.55 respectively. The developed parameters can be used as a standardization tool for B. suffruticosa whole plant. Keywords: Bergia suffruticosa, Pharmacognostic P-190: PHARMACOGNOSY CONCEPTS IN AYURVEDA AND AYURVEDIC DRUG STANDARDIZATION Dilip K Jani*, ARV Murthy
G. J. Patel Institute of Ayurvedic Studies & Research and S. G. Patel Ayurved Hospital & Maternity Home, New V.V. Vidyanagar, Gujarat The subject of herbal drug standardization is massively wide and deep. There is so much to know about the theories on the subject of herbal medicines and its relationship with human physiology and its function. In earlier days, the activity of herb procurement, preparation and dispensing remained mainly the responsibility of practitioners and was on a one to one relationship between physician and his patients. It was a matter of sacred trust. However, the socio-economic changes in modern times, the technological advances, commercial factors, consumer preferences, changing lifestyles, etc. has influenced a lot. Standardization is also used to provide a reliable dosage, their performance, limitations, optimal dosages, contra-indications, and applications. Now a day it is important to have consistency and herbal drugs must also be practiced and applied in a uniform way. The WHO Guidelines and Ayurvedic Monogram highlights on Authentication of the herbal drug. This includes time of collection, stage of collection, parts collected, regional status, taxonomical identity like morphology and microscopical analysis. The guidelines also include the study of foreign matter (herbs collected should be free from soil, insect parts or animal excreta, etc.), the ash and extractive values. The chemical characteristics by Chromatographic evaluation also must be taken into consideration. Determining the heavy metals like cadmium, lead, arsenic, etc in the drug can also assess the quality of the drug. The description regarding identification of drugs can be traced back from the Vedic literature. Identifying methods of some external features of the drugs are explained in Rigveda, and in Athrvaveda. The description of the life history of a plant and comparison of internal structure of a plant and human anatomy explained in Brihadakaranya Upanishad. Many of our Samhitas and Nighantus explained the description of morphology of plants by adding synonyms. The use of thousands of herbs as medicines has been described in detail in traditional system of medicine like Ayurveda. These descriptions include identification, collection, processing and its therapeutic application etc. It is found that if there is strict adherence to these guidelines of traditional concepts will ensure the quality of the finished products and only such products will alleviate the diseases they are intended to cure. Acharya Charaka also explained the methods of identification and standardization in Ayurveda. He also explained that the unknown drug might act as a poison or as a weapon or like fire. One good character of a physician established by him is that one must possess complete knowledge on identification of drugs and their therapeutic uses. For the identification of drugs, Charaka advised to take the help from cowherds, foresters, and Sages etc. Charaka and Susruta classified the drugs into 50 and 37 groups respectively on the basis of pharmacological and therapeutic utility. Classical evaluation of the drug as per Ayurvedic therapeutics attributes to the assessment of Rasna, Guna, Veerya, Vipaka, Karma, Prabhava, Matra, etc. It also takes into consideration of the nomenclature as well as for identification, like Rudhi (Allergy), Swabhava (Habit), Deshoktya (Habit), Lanchhana (Morphological), Upama (Smile / Comparing), Veeryena (Potency) and Itharathwa (Miscellaneous) of the specified drug. Keywords: Pharmacognosy, Ayurveda, Herbal, Standardization P-191: HEPATOPROTECTIVE ACTIVITY OF SEEDS OF CASSIA TORA LINN. CHITTAM K.P., PATIL M.S., PATIL S.B., WAGH R.D. A.R.A. College of Pharmacy, Dhule, Maharashtra In present study, all the three chloroform, methanol and ethyl acetate extracts were evaluated for hepatoprotective activity of seeds of Cassia tora Linn. against the paracetamol induced hepatotoxicity in albino wistar rats of either sex. The extracts at the dose of 400 mg/kg were administered orally. Ethyl acetate extract showed significant results for the elevated levels of SGOT, SGPT, SALP and Serum bilirubin when compared with Liv-52 used as a standard drug. Chloroform and methanol extract also showed the moderate results for the elevated levels by reducing it but to the lesser extent than the ethyl acetate extract. Keywords: Cassia tora, Hepatoprotective activity, Paracetamol, Chloroform P-192: LIMITATIONS OF CONVENTIONAL DRUG DISCOVERY METHODS AND OPEN OPTIONS TO EXPLORE AYURVEDA Dilip K Jani, ARV Murthy G. J. Patel Institute of Ayurvedic Studies & Research and S. G. Patel Ayutrved Hospital & Maternity Home, New V. V. Nagar In the fields of medicine, biotechnology and pharmacology, drug discovery is the method by which drugs are revealed or designed. In the current search for novel drugs against disease, scientists are making use of new approaches to drug discovery that aspire to increase the rate of success, reduce the development timeline and diminish the cost. The process of drug discovery involves the detection of candidates, synthesis, characterization, screening, and assays for therapeutic efficacy. Once a compound has revealed its value in these tests, it will begin the process of drug development prior to clinical trials. Drug discovery research involves significant resources and investment from multiple scientific research units and venture capitals at diverse phases of early drug research. In the past most drugs have been discovered either by identifying the active ingredient from traditional remedies or by unexpected discovery. Conventional drug discovery approaches involve high-throughput screening of up to millions of large compounds to find those that inhibit the activity of the target, usually a protein. Although many drugs have been discovered this way, the technique is expensive and resource intensive, and there is a high attrition rate of hits failing to progress through the development process to medicines., Despite advances in technology and understanding of biological systems, drug discovery is still a lengthy, "expensive, difficult, and inefficient process" with low rate of new therapeutic discovery.
Currently, the research and development cost of each new molecular entity (NME) is approximately US$1.8 billion. The conventional methods lack predistinct metrics to measure process performance to take appropriate initiatives for improvement, productivity of assets, people and inventory quality in terms of the research output and data and cycle time to assess the time spent on non value added activities. In recent times the discovery phase has become a prime focus of the improvement initiatives of the pharma industry. Industry has begun to realize the principle of designing quality at early stage rather than relying upon results of clinical studies and believing in the end product testing. Drug discovery strategies based on Ayurveda drugs and natural products are re-emerging as attractive options. It is observed that drug discovery and development need not always be confined to new molecular entities. Rationally designed, carefully standardized, synergistic traditional herbal formulations and botanical drug products with vigorous scientific evidence can also be alternatives. A reverse pharmacology approach, inspired by traditional medicine and Ayurveda, can offer a smart strategy for new drug candidates to facilitate discovery process and also for the development of rational synergistic botanical formulations. Keywords: Ayurveda, Drug Discovery, Pharmacology P-193: ANTHELMINTIC ACTIVITIES OF THE CRUDE EXTRACTS OF ALLIUM CEPA BULBS AND ELLETATRIA CARDOMOMUM SEEDS AA Bidkar , C Patel , MG Hadiali1, K N Gujar2 1Sinhgad Institute of Pharmacy,Pune; 2Smt. Kashibai Navale College of Pharmacy, Pune Crude extracts of Allium cepa bulbs and Elletatria cardomomum seeds show strong anthelmintic activity on Pheretima Posthuma (earthworm). The ethanolic extracts of Allium cepa are more potent than the corresponding aqueous extracts; while, the aqueous extracts of Elletatria cardomomum are more potent than the corresponding methanolic extracts. The anthelmentic activity has a linear relationship with the dose of the extract used. For all the extracts the earthworms were paralysed within 20 mins and died within 30 mins of exposure. The extracts of Elletatria cardomomum are found to be more potent than the extracts of Allium cepa. Keywords: Anthelmentic, Allium cepa bulbs, Elletatria cardomomum seeds, earthworm P-194: RATIONALITY FOR SELECTION OF HERBS FOR AYURVEDIC FORMULATION USED IN GI DISORDERS Parmar Neha, B.R.Patel IPGT&RA, Gujarat Ayurveda University, Jamnagar In Ayurvedic system of medicine multi-drug formulations are used predominantly than single drug. Majority of the diseases involve multiple symptoms and systems of the body and for its complete treatment application of multiple herbal formulations becomes essential. Rationale for selection of herbs in Ayurvedic formulation is based on different etiological factors i.e., Dosha, Dushya, Strotas, etc. and properties of drugs i.e., Rasa, Guna, Veerya, Vipaka, Prabhava and its synergy. In this study, 34 classical herbal formulations used in 17 Gastro intestinal tract disorders like indigestion, dysentery, diarrhea, vomiting, haemorrhoids etc. will be critically analyzed and an effort will be made to find out rationality for selection of drug based on Ayurvedic basic fundamentals. Keywords: Herbal formulation, GI tract disorders P-195: PHARMACOGNOSTICAL EVALUATION OF HOLOPTELEA INTEGRIFOLIA (ROXB.) PLANCH LEAF Sushama B Bhuvad, Harisha C R, RN Acharya, K Nishteswer IPGT&RA, Gujarat Ayurveda University, Jamnagar Holoptelea integrifolia (Roxb.) Planch belonging to the family Ulmaceae, is a large, deciduous tree. Leaves are elliptic-ovate, acuminate, base rounded or sub-cordate. Flowers greenish yellow, in short racemes or fascicles on the leafless branches. Fruit sub-orbicular samara with membranous wing. Seed is flat. It is found throughout the greater part of the India up to an altitude of 660m. The bark and leaves of this plant is used in medicinal preparations by Ayurvedic physicians in treating inflammation, acid gastritis, dyspepsia, flatulence, colic, wounds, skin disease, vitiligo and worms. Seeds are useful in infected ulcers. The pharmacognostical and phytochemical characterisation of the plant bark has already been reported but the leaves are yet to be studied.This paper presents macroscopic, microscopic and powder microscopy of leaf of Holoptelea integrifolia (Roxb) Planch, as per the guidelines of Ayurvedic Pharmacopoeia of India (API). Key words: Holoptelea integrifolia (Roxb.) Planch, Pharmacognosy P-196: ANTI - LIPID PEROXIDATIVE PROPERTY OF SOME PHYTOCONSTITUENTS IA Najar, SC Sharma, RK Johri PK/PD-Toxicology Department Indian Institute of Integrative Medicine (C.S.I.R), Jammu The transition metal iron plays a key role in the production of reactive oxygen radicals in vivo. In the present study, the effect of certain phytoconstituents present in known medicinal plants on reactive oxygen species (ROS) scavenging has been evaluated using rat liver homogenate. Lipid peroxidation (unstimulated and Fe2+/ascorbate stimulated) was determined by thiobarbituric acid reaction. In this context, the status of acteoside, berberine, catechin, 3´5-dihydroxyflavone7-o-ß-D-galacturonide-4-o-ß-D-glucopyranoside (a flavonoid glycoside from cumin), and silibin decreased both basal and stimulated lipid peroxidation. On the other hand agnuside, andrographolide, picroside-I, negunoside, oleanolic acid, and glycerrihizin caused an
increase in both basal and stimulated lipid peroxidation. Chlorogenic acid, apocyanin, santonin, piperine, tetrahydrocurcumin, and picroside-II did not show significant effect. Keywords: reactive oxygen species, lipid peroxidation, phytoconstituents, basal P-197: COMPARITIVE IN VITRO ANTI CANCER ACTIVITY OF COCCULUS HIRSUTUS AND CISSAMEPLOS PAREIRA B. Samuel Thavamani PSG College of Pharmacy, Coimbatore, Tamilnadu Menispermaceae, the botanical name for a family of flowering plants, has been universally recognized by taxonomists. It is a medium sized family of 70 genera totaling 420 extant species, mostly of climbing plants. Cocculus hirsutus and Cissampelos pareira both beloning to this family is taken for the present study. The objective of this study is to have a comparative In vitro anti cancer activity of Cocculus hirsutus and Cissampelos pareira in HeLa and human liver cancer cell lines. The human cervical cancer cell line (HeLa) and human liver cancer cell lines were obtained from National Centre for Cell Science (NCCS), Pune, and grown in Eagles Minimum Essential Medium containing 10% fetal bovine serum (FBS). All cells were maintained at 370C, 5% CO2, 95% air and 100% relative humidity. After 48h of incubation, 15µl of MTT (5mg/ml) in phosphate buffered saline (PBS) was added to each well and incubated at 370C for 4h. The medium with MTT was then flicked off and the formed formazan crystals were solubilized in 100µl of DMSO and then measured the absorbance at 570 nm using micro plate reader. The % cell inhibition was determined. Nonlinear regression graph was plotted between % Cell inhibition and Log10 concentration and IC50 was determined using GraphPad Prism software. The IC50 value of Cocculus hirsutus in g respectively.g and 54.36Hela and liver cancer cell line was found to be 111 Similarly the IC50 value of Cissampelos pareira in Hela and liver cancer cell g respectively.g and 85.62line was found to be 129.3 The above result shows that the anti cancer activity of Cocculus hirsutus in liver cancer cell line was found to be significant. Keywords: Cocculus hirsutus, Cissampelos pareira, anti cancer P-198: NUTRACEUTICALS - AN AYURVEDIC APPROACH Sujeet K Sagar, BR Patel IPGT&RA, Gujarat Ayurveda University, Jamnagar The terms food supplement, dietary supplement and health supplement are commonly used and practiced in day to day life. All these are covered under the term nutraceutic. "Nutraceutical is a food or food product that reportedly provides health and medical benefits, including the prevention and treatment of disease." The current trend of promoting the indiscriminate use of nutraceuticals is showing untoward health effects in individuals for e.g.- Adverse reaction to soy like skin reactions, lung congestion, digestive problems, life threatening reaction and many more1. This calls ones attention to the concepts of Ayurvedic system in the development of nutraceuticals. The concept of dietetic substances and its utilization has been described in detail in Ayurvedic texts by using different Ayurvedic terminology i.e. Anna-Pana Dravya Jivaniya, Bringhniya, Rasayana, Vajikar, Balya, etc. The selection of food and health supplements should be based on specific Ayurvedic parameters i.e. Prakriti, Desh, Kala etc. which varies from person to person. In present study an attempt has been made to analyze and evaluate different Ayurvedic herbs which can be used as food and health supplement. Keywords: Nutraceutics, Ayurvedic approach, Medicinal plants P-199: CNS ACTIVITIES OF DIFFERENT PLANT SOURCES OF SHANKHPUSHPI: A COMPARATIVE STUDY Jai Malik, Maninder Karan, Sunayna Choudhary, Karan Vasisht Department of Pharmacognosy, ISF College of Pharmacy, Moga, Punjab Objective: Shankhpushpi is a well established Medhya Rasyana (nervine tonic) in Ayurveda. Despite a popular drug, different plants are used under the name Shankhpushpi in different regions of India, leading to uncertainty regarding its true source. Plants commonly used under the name Shankhpushpi are: Convolvulus pluricaulis Chois, Evolvulus alsinoides Linn. (Convolvulaceae) and Clitoria ternatea Linn. (Leguminosae). To find out the true source of shankhpushpi the above mentioned three plants were compared for their memory enhancing, anxiolytic, antidepressant and CNS depressant activities. Materials and methods: The hydromethanol extract of different plants was used to study various CNS activities. The memory enhancing activity of each plant was tested using elevated plus maze (EPM) and step down models. Anxiolytic, antidepressant and CNS depressant studies were evaluated using EPM, Porsolt's swim despair and actophotometer models, respectively. Results: C. pluricaulis extract(CPE) at a dose of 100 mg/kg, p.o. showed maximum nootropic and anxiolytic activity (P<0.001). E. alsinoides extract (EAE) and C. ternatea extract (CTE) showed maximum memory enhancing and anxiolytic activity (P<0.001) at 200 and 100 mg/kg respectively. Amongst the three plants, EAE and CTE showed significant (P<0.05), while CPE did not exhibit any antidepressant activity. All the three plants showed CNS depressant action at higher dose levels. Conclusion: C. pluricaulis exhibited maximum memory enhancing and anxiolytic activity followed by C. ternatea and E. alsinoides. The results of the present study suggest that C. pluricualis should be used as true source of Shankhpushpi. Keywords: Ayurveda, Convolvulus pluricaulis, Clitoria ternatea, Evolvulus alsinoides
P-200: WOUND HEALING ACTIVITY OF LEAVES EXTRACT OF CASSIA OCCIDENTALIS LINN. Nilesh Gupta & Umesh K Jain Bhopal Institute of Technology & Science-Pharmacy Methanolic extract of leaves of Cassia occidentalis were evaluated for wound healing activity. Extract in the form of ointment is applied topically on excision, incision and dead space models in mice showed significant healing process as evidenced by increase rate of wound contracting ability, wound closure time, tensile strength and dry granuloma weight as compared to control. The methanolic extract of 5% w/w ointment exhibit equivalent wound healing activity as Povidine iodine. Histological analysis of the tissue obtained on day 13th from the extract treated group showed increased well organized bands of collagen, more fibroblasts, macrophage and blood vessels compared to control, which also consistent with the proposal that Cassia occidentalis leaves extract exhibits significant wound healing activity. Keywords: Keywords Wound healing activity, Cassia occidentalis, Povidine iodine P-201: ANTICONVULSANT ACTIVITY OF LEAVES OF CAPPARIS DIVARICATA LAM. Patil MS, Patil SB, Chittam KP, Wagh RD A.R.A. College of Pharmacy, Dhule, Maharashtra The aim of the present study was to evaluate the anticonvulsant activity of ethanolic extract of leaves of Capparis divaricata Lam. in experimental animals. The activity was evaluated against PTZ induced convulsions in mice. Oral administration of ethanolic extract of leaves of Capparis divaricata Lam. at the dose of 250 mg/kg and 500 mg/kg produced significant (P< 0.01) protection by prolonging the latency period of convulsions as compared with the control group. It prolongs the onset of action from 52.83 sec. upto159.1 sec. and 199.60 sec.at the dose of 250 and 500 mg/kg respectively. It may conclude that the ethanolic extract controls the myoclonic and absence seizures as it protects the animals against ptz induced convulsions. Preliminary phytochemical screening showed the presence of alkaloids, flavonoids, phenolic compounds, glycosides, tannins and saponins. It shows the need for further study to determine the exact mechanism of action and isolation of active principle(s) from ethanolic extract of leaves of Capparis divaricata Lam. for anticonvulsant activity. Keywords: Capparis divaricata, anticonvulsant activity, PTZ, Ethanol, mice P-202: PHARMACOGNOSTICAL EVALUATION OF CORDIA MACLEODII HOOK LEAF THROUGH DNA FINGER PRINTING Ashish kumar sharma, Rabinarayan Acharya, VJ Shukla, Harisha CR Institute for PG Teaching and Research in Ayurveda, Gujarat Ayurved Uni., Jamnagar Cordia macleodii Hook (Ethretiaceae), a Folklore medicinal plant, used for wound healing, to treat Rheumatism and Hepato-protective activity, by the tribal peoples of Orissa and Madhya Pradesh. Other species of the Genus Cordia such as C myxa and C dichotoma i.e. are also used as source plant of different Ayurvedic medicinal plantssuch as Shleshmatak and Laghu Shleshmatak. In this research paper an attempt has been made to study the Pharmacognostical charectors of Cordia macleodii leaf and carry out the DNA studies through RAPD method. The leaf of this plant shows epidermis, trichomes, mesophyll and semilunar bicollateral vascular bundles. The powder microscopy of leaf shows presence of trichomes, epidermal cells, stomata and yellowish brown tannin content. The fresh leaves are subjected to DNA studies through RAPD method. The detailed of this study shall be presented during the time of presentation. Keywords: Cordia macleodii, Pharmacognosy, RAPD, Tribal medicine P-203: EVALUATION OF HEPATOPROTECTIVE ACTIVITY OF ROYLEA ELEGANS (AERIAL PARTS) Nitika Chauhan, Gaurav upadhyay, Rahul Deshmukh, Jai Malik Department of Pharmacognosy, ISF College of Pharmacy, Moga, Punjab Objective: The aerial parts of Roylea elegans (RE) are widely used against liver disorders in various tribal areas. Till date, no scientific work has been carried out to validate its traditional use. Therefore, the present investigation was designed to validate the ethnopharmacological claims of the drug. Method: The hydroalcoholic extract of R. elegans (100, 200 & 400 mg/kg, p.o.) was evaluated for its hepatoprotective activity against CCl4 (1:1 ratio of CCl4 and olive oil, s.c.) and PCM (3 mg/kg, p.o.) induced hepatotoxicity in Wistar rats. The effect was compared with control and standard drug silymarin (50 mg/kg, p.o.) treated animals. The hepatoprotective effect was evaluated by estimating the serum levels of SGPT, SGOT, ALP and total billirubin, tissue parameters (TBARS and reduced Glutathione), and by histological studies. Results: Hydro-alcoholic extract exhibited a dose dependent hepatoprotective effect against both the toxicants. R. elegans extract (200 mg/kg and 400 mg/kg) showed significant (P<0.05) hepatoprotective activity in comparison to the control and toxicant treated animals. Moreover, the hepatoprotective effect of the above two dose levels was comparable to that of silymarin in terms of serum levels of SGOT, SGPT, ALP and total billirubin. Furthermore, the extract also exhibited antioxidant activity which was evident from TBARS and glutathione levels. Histopathological studies also indicated towards the hepatoprotective effect of the lyophilized extract (200 mg/kg and 400 mg/kg) which was indicated by the reversal of CCl4 and PCM induced ballooning and tissue necrosis. Conclusion: The present investigations clearly indicate the hepatoprotective effect of RE against CCL4 and PCM induced hepatotoxicity and validating its traditional use for liver disorders. Keywords: Hepatoprotective activity, Roylea elegans, Liver disorder
P-204: HPTLC AND HPLC METHODS FOR DETERMINATION OF LUTEOLIN IN HERBAL EXTRACT AND FORMULATION CONTAINING ECLIPTA ALBA LINN. Rashmi Uniyal, Pranav Kumar Yadav, Vinod Gauttam, AN Kalia Department of Pharmacognosy, ISF College of Pharmacy, Moga, Punjab Eclipta alba (Asteraceae) is traditional medicinal plant known as Bhringaraj and exhibit hepatoprotective, anthelmintic, anticancer, antihyperglycemic, antidiabetic, anti-inflammatory, antioxidant and memory enhancing activity. It is used as an ingredient in number of marketed herbal formulations. Luteolin is one of the major constituent present in Eclipta alba. The experiment was designed to develop a simple, selective, precise and reproducible high performance thin layer chromatography (HPTLC) method for the analysis of luteolin in herbal extracts and formulation(s) containing Eclipta alba. The study was performed on aluminium backed precoated TLC plates (Silica gel 60F254) as the stationary phase. Ascending development of the chromatograms were allowed in twin trough glass chamber pre-saturated with mobile phase comprised of toluene: ethyl acetate: formic acid (5: 3.5: 0.1, v/v/v) at room temperature (25±2°C). The densitometric analysis was done by TLC scanner III (CAMAG) in absorbance mode at the wavelength of 351 nm. The system was found to give compact spots for luteolin (Rf value of 0.34±0.002). The data obtained from linear regression data for the calibration plots showed good linearity (r2 = 0.99601±0.002) with respect to peak area in the concentration range of 200-1200 ng spot-1. According to the International Conference on Harmonization (ICH) guidelines the method was validated for its accuracy, precision, recovery, and robustness. The limits of detection and quantitation (20 and 60 ng spot-1) were also established. The method has been successfully applied in the analysis of herbal extract and market formulations containing luteolin. Keywords: HPTLC, Eclipta alba, Validation, luteolin P-205: OPTIMIZATION AND STANDARDIZATION OF ANTIDIABETIC POLYHERBAL FORMULATION Meenakashi Rohilla, Swati Manik*, Vinod Gauttam, AN Kalia I.S.F. College of Pharmacy, Moga, Punjab The present study was undertaken to evaluate the antidiabetic activity of polyherbal combinations of three well known medicinal plants used in traditional medicines (Momordica charantia, Gymnema sylvestre, Withania somnifera) and to standardize the developed formulation using HPTLC technique. The aqueous and hydroalcoholic (50 % methanolic) extracts of the individual drugs were prepared and selected on the basis of percentage yield and on the phytochemical analysis. The best selected extract was combined in different proportion. The best ratio was optimized on the basis of OGTT study at the dose of 1000 mg/ kg b.w. The optimized formulation was further studied for its antidiabetic properties in alloxan-induced diabetic rats for 21 days at two dose levels. The results (blood glucose level, lipid profile, liver glycogen and body weight) were compared with the marketed formulation having more than 12 different components and with the standard drug metformin. The optimized formulation was standardized, using HPTLC fingerprinting profile. The HPTLC method was developed for the simultaneous estimation of vicine and withaferine (markers) to estimate their amount in the optimized combination which further helps in the standardization of the proposed formulation. The observation revealed that the optimized polyherbal combination has shown significant (P<0.05) antihyperglycemic and antihyperlipidemic activities. The results were comparable with the standard and the marketed polyherbal formulation containing these three drugs along with several other components. Conclusion: - The optimized formulation with these three components has shown antidiabetic activity at par with the marketed polyherbal formulation, suggesting the unnecessarily load of herbal drugs can be avoided, moreover easy to standardize and fulfil the FDA requirements for export. Keywords: Polyherbal formulation, OGTT, Standardization, Alloxan, Antidiabetic P-206: EVALUATION OF ANTINFLAMMATORY ACTIVITY OF CASSIA OBTUSIFOLIA (LEAVES) Suman Malik, Prarthi Mehta, AN Kalia Department of Pharmacognosy, ISF College of Pharmacy, Moga, Punjab Objective: The leaves of Cassia obtusifolia (CO) are widely used against inflammation in traditional system of medicine. Till date, no scientific work has been carried out to validate its traditional use. Therefore, in order to validate the traditional claim, the present investigation was designed to evaluate its anti-inflammatory effects. Method: Pet ether and methanolic extracts were prepared by using soxhlation and aqueous extract was prepared by triple meceration. The dose was selected on the basis of acute toxicity study, at the highest dose of 2000 mg/kg there were no behavioral or toxic manifestations and mortality been observed. petroleum ether, methanol and aqueous extracts (250 and 500 mg/kg) were studied for anti-inflammatory activities on carageenan induced paw edema.the most active methanol fraction was further fractionated and studied for its anti-inflammatory activity on carrageenan and cotton pellet induced edema in rats. The results were compared with standard drug diclofenac. Results: The methanolic extract has shown significant anti-inflammatory property.anti-inflammtory activity was further potentiated with n-butanol fraction of methanolic extract. Nbutanol at (100mg/kg) has shown significant activity both in acute and chronic models and results were comparable with standard drug diclofenac(20mg/kg) and n-butanol fraction of methanol extract (50 and 100 mg/kg) showed significant (P<0.05) inhibition of paw edema . The maximum inhibition in edema volume was showed by n-Butanol fraction at a dose of 100 mg/kg, comparable to standard drug diclofenac sodium(20mg/kg). The phytochemical screening of n-butanol fraction has revealed presence of triterpenoids
and flavonoids. Conclusion: the study has confirmed the traditional use of the leaves of (CO). the activity may be due to terpenoids present in n-butanol fraction. Further work is in progress. Keywords: anti-inflammatory, Cassia obtusifolia P-207: EVALUATION OF COMPARATIVE PHARMACOGNOSTICAL AND PHOTOCHEMICAL STUDY OF ROOTS OF WILD AND CULTIVATED MUCUNA PRURIENS HOOK Dadhaniya SK1, Gokani RH2, Rachchh MA2 1Smt. R. B. Patel Mahila Pharmacy College, Atkot; 2SJ Thakkar Pharmacy College, Rajkot Mucuna pruriens Hook (Fabaceae) commonly known as Kivach, Cow-hage and Velvet bean is indigenous to tropical countries like of India, West Indies and South Africa. Traditionally, the roots of M. Pruriens are used as a bitter tonic, purgative, aphrodisiac, diuretic, anthelmentic, nerve tonic. In India, it is cultivated as well as is also found growing wildly. During market surveillance of herbal drug, it was observed that both wild (Black seeded) and cultivated (White seeded) varieties roots of M. pruriens are available. But so far their comparative pharmacognostical and phytochemical activities are not reported yet. Therefore, in this context the detailed pharmacognostical evaluation of both the roots has been carried out with the aim to establish the identification markers of roots of wild and cultivated roots of M. pruriens. The pharmacognostic evaluation were carried out in terms of organoleptic, microscopic and physical parameters. The extracts obtained after successive Soxhlet extraction of both the root powder using different solvents, were subjected to a preliminary phytochemical screening which revealed the presence of carbohydrates, proteins, phenolics, alkaloids, flavonoids. The present comparative study explores that cultivated roots of M. pruriens has more powerful phytochemical profile so, this data will be essential to validate suggested comparative advantages and may provide new options for plant breeding. Keywords: Mucuna pruriens, Wild and Cultivated P-208: PHARMACOGNOSTICAL STUDIES OF RUELLIA PROSTRATA POIR AND ITS BIOLOGICAL EVALUATION FOR ANTI-INFLAMMATORY POTENTIAL IN WISTAR ALBINO RATS Roopa, Varinder Singh, Jyotiram Sawale, Rohit Goyal, AN Kalia Department of Pharmacognosy, ISF College of Pharmacy, Moga, Punjab The aerial parts of Ruellia prostrata Poir are used as anti-inflammatory, anti-cancer and anti-arthritic in traditional system of medicine. Ruellia species contain chemical constituents lupeol, stigmasterol, flavonoids, saponins and -sitosterol which are reported to have anti-inflammatory properties. Therefore, in order to validate the traditional use of plant, the present study was designed to evaluate its anti-inflammatory effects. Morphological characters showed leaf- petiolate, ovate-lanceolate, base acute, apex sub-acute, hairy, stem hairy, purple at nodes, fruits covered with club shaped capsule with fine hairs. T.S. of leaf showed cuticle followed by single layer of epidermal cells on both upper and lower surface and 4-5 layer of spongy parenchyma. T.S. of stem showed pith, xylem vessel, cortex, medullary rays. Powder microscopy showed tricellular covering trichomes, lignified vessels, diacytic stomata. The phytochemical screening of methanolic and aqueous extract of aerial parts showed presence of carbohydrates, steroids and tri-terpenoid glycosides. The methanolic extract showed significant anti-inflammatory activity than aqueous extract at dose of 500mg/kg in prevention of edema and granuloma in carrageenan induced rat paw edema and cotton pellet-induced granuloma, respectively. The n-butanol fraction of methanolic extract at dose of 125mg/kg showed marked inhibition of paw edema in carrageenan induced rat paw edema animal model. The n-butanol fraction has already been proved in our lab for its anti-oxidant properties. It can be concluded that the anti-inflammatory properties of plant may be due to its antioxidant potential. Hence, present study confirms the anti-inflammatory potential and validate the traditional use of plant. Keywords: Ruellia prostrata, anti-inflammatory P-209: AYURVEDIC PERSPECTIVES ON HERBAL GALACTOGOGUES Harshitha kumari, Malati G Chauhan, A P G Pillai, Nishteswar K Institute for Post Graduate Training & Research in Ayurveda, Gujarat Ayurved Uni., Jamnagar Ayurveda is one of the most indigenous and developed systems of Indian medicine having a very comprehensive materia medica enumerating various pharmacological actions. Acharya Charaka, one of the prominent physician has enumerated 10 drugs having stanyajanana (galactogogue) property i.e., Veerana (Vetiveria zizanoids), Darbha (Desmostachya bipinnata), Kasa (Saccharum spontaneum) etc. He has also introduced a novel pharmacological action ie stanya sodhana ( lactation purifiers) and delineated 10 drugs viz., Pata (Cissampelos pereira), Mahoushadha (Zingiber officinale), Suradaru (Cedrus deodara), Musta (Cyperus rotundus), Guduchi (Tinospora cordifolia), Kiratathikta (Swertia chirata) etc. Certain herbs enumerated under stanyajanana and stanya sodhana activity may influence the hormone i.e., progesterone and prolactin. These plants may be considered as phytoprogesterones. A thorough scan of Ayurvedic works of medieval India included certain herbs namely Satavari (Asparagus racemosus), Vidari kanda (Pueraria tuberosa), Yashtimadhu (Glycyrrhiza glabra) for improving lactation. Guidelines like time of administration, dose, pharmaceutical form are emphasized for effective drug delivery and enhancing the pharmacological action in Ayurveda. Different research studies confirmed the galactogogue property of Satavari (A. racemosus) and Jivanti (L.reticulata). In the present paper an attempt will be to highlight the Ayurvedic pharmacology with reference to galactogogues. Keywords: Galactogogue, Asparagus racemosus, Leptadenia reticulata
P-210: A SCIENTIFIC PHARMACOGNOSTICAL APPROACH ON VARIOUS PARTS OF JYOTISMATI (CELASTRUS PANICULATUS WILLD.)- A POLYGAMODIOECIOUS PLANT Monojit debnath, K Nishteswar IPGT&RA, Gujarat Ayurved University, Jamnagar Nature has its own rule to protect every life of this universe. It incorporates various valuable medicinal products in various plant parts. Jyotismati, a very demanding plant in traditional system of medicine, is a woody climber and used by the civilization from the Ayurvedic Samhita periods. It is found that the male and female plant exists separately in this species (dioecious) and the male and female flower also exist together in the same plant (polygamous); the particular type of plants botanically named as Polygamodioecious plant (eg-Guggulu). Numbers of experiments based on tranquilizing, sedative, memory learning, antirheumatic etc. action of its seed oil have been done. Due to a rapid use of the seed the species became endangered. So a criterion to find out the alternative one by means of substitute is very necessary for today. In the present study detail pharmacognosy of every part of jyotismati apart from the seed have been done. It is found that like the seed every part of this plant contains oil that is basically fixed oil. Leaf section shows a plenty of oil globules through the mesophyll, upper and lower epidermis region also. A 12 hour continuous process in Clevenger apparatus was carried on, and it results as no volatile oil is present in leaf. So the oil contains in the leaf is fixed oil. Tribal claim illustrates leaf's sedative action and research work proves the leaf juice is more effective than seed oil in case of rheumatoid arthritis. So it can be concluded that may be the properties of leaf containing oil is similar to some sort of the seed oil and can also be used as a substitute of it. Various research works and ideas may be organised basing on the results of details pharmacognosy of jyotismati which is actually performed in the present study. Keywords: Jyotismati, detail microscopy, leaf oil, Celastrus paniculatus P-211: REVIEW ON AMALAKI (EMBLICA OFFICINALIS GAERTN. ) AND ITS EFFECTIVE FORMULATIONS IN AYURVEDA: A POTENT CELL PROTECTOR Reshmi Pushpan, Malati G Chauhan, A P G Pillai, Nishteswar K Institute for Post Graduate Training & Research in Ayurveda, Gujarat Aurved Uni., Jamnagar Ayurvedic materia medica is replete with several safe and effective rasayanas (herbal immune modulators). The concept of Rasayana (rejuvenator/ anti oxidants) and Balya (immune modulators) activities mainly act on preserving the cell integrity and immunity. Recent research studies have confirmed the immune enhancing activity of Atibala ( Abutilon indicum) among Bala group of drugs. Acharya Charaka has attributed significant Vayasthapana (youth restorative) activity of Indian Gooseberry (Amalaki). Initial phytochemical studies have reported that Amla contains heat labile ascorbic acid (Vitamin C). In another study carried out by Prof. Goshal, it was observed that Amla does not contain Vitamin C at all and its activity should be explained by two components namely, Emblicanin A and Emblicanin B. A thorough review of Amla and its specific formulations indicated in improving immunity will be reviewed in view of its significant clinical application. Keywords: Immunomodulator, Emblica officinalis, Rasayana P-212: DEVELOPED NATURAL SKIN CARE PRODUCTS FROM TRADITIONAL AND HERBAL MEDICINES Shyam Bihari Sharma School of Studies in Pharmaceutical Sciences, Jiwaji University,Gwalior, Madhya Pradesh Traditional skin remedies and beauty aids were perfected over the centuries of trials. Herbal skin care products offer balanced combination of natural anti-aging and nourishing nutrients and antioxidants easily absorbed by the skin. Herbal bath and body products help nourish, restore, revitalize the skin and promote the healing of skin conditions. Herbal oils were traditionally obtained by extraction of dry herb with a vegetable oil. Herbal oils are unique source of herbal nutrients, antioxidants and vitamins essential for cells metabolism. They have been used in herbal medicine and cosmetics since ancient times to maintain healthy skin and scalp, treat skin problems, help heal skin injuries, improve the conditions of mucous, combat scalp and hair diseases.All natural massage oils comprise herbal extracts, herbal essences and natural vitamins used to soften, protect and moisturize the skin.Hair oils moisturize scalp, reverse dry scalp and dry hair conditions, provide nutrients required to maintain normal functions of sebaceous glands and promote natural hair growth. Hair oils have been traditionally used to treat irritated stressed scalp, reduce effect of aging on hair shape and growth, combat seborrhea and alopecia.Home remedies for glowing skin are used in India since ages. Keywords: Herbal skin care products, Herbal oils P-213: MEDICINAL USES OF ARECA CATECHU Ravichandra V1, Shyamala K V2, Pallavi2 1Melaka Manipal Medical College, Manipal University; 2Kasturba Medical College, Mangalore Arecanut (betelnut) chewing habit dates back to antiquity. Arecanut mixture taken after meals sweetens the breath producing energizing effect and euphoria. Major constituents of arecanut include polyphenols, tannins, fats and alkaloids. Of all the alkaloids, arecoline is the main active component with potent cholinergic activity resembling muscarine. Recent reports of harmful effects of chewing arecanut are produced only after prolonged years of repeated chewing habit. Apart from its masticatory use, it also has considerable medicinal uses as well. Arecoline was incorporated into British Pharmacopeia and Indian Pharmacopeia due to due to its anthelmintic properties. Arecanut or supari constituents have laxative and diuretic effect. Arecanut has astringent principles and prevents tooth decay. Though there is little clinical evidence to support the clinical use of arecanut, it might
help those suffering from Alzheimer's disease and other psychological disorders such as schizophrenia. Therefore, an account of medicinal uses of arecanut as per three systems of medicine viz., Ayurveda, Yunani,Sidda and modern research work done on its medicinal properties is beingpresented. P-214: ANTIOXIDANT PROPERTIES OF SOME THERAPEUTICALLY ACTIVE TRADITIONAL MEDICINAL PLANTS - AN OVERVIEW Jalpa R Thakkar, Shila N Gevariya, HP Gajera, SV Patel, BA Golakiya Department of Biotechnology, Junagadh Agricultural University, Junagadh, Gujarat Recent years have witnessed a renewed interest in plants as pharmaceuticals. This interest focused not only on the discovery of new biological active molecules by the pharmaceutical industry, but also on the adoption of crude extract of plants, such as infusions, for self medication by general public. With this context, considerable interest has arisen in the posibility that impact of several diseases may be prevented by improving the dietary intake of natural nutrients with antioxidant properties, such as vitamin E, vitamin C, carotene, tannins, flavonoids etc. The approach of phytochemicals in medicinal plants have attracted a great deal of attention mainly concentrated on their role in preventing diseases which may, otherwise, caused as a result of oxidative sttress. Oxidative stress which release free oxygen radicals in the body has been implicated in a number of disorders including cadiovascular malfunction, cataracts, cancers, rheumatism and many other autoimmune diseases. Detoxification of ROS by antioxidants affords protection against such diseases. Phytochemicals including phenolics, flavonoids, lycopene, carotenoids and glucosinolates have been shown to have potent antioxidant properties against free radicals. The herbal preparations of traditional medicine may have an antioxidant activity arising from their content of plants with antioxidant principles, which act probably in a synergistic way. Present paper overviews the antioxidant effects of active principles of some traditional medicinal plants which may benefit as anti-stress, immuno-modulatory, cognition facilitating, antiinflammatory and anti-ageing effects as reported in experimental animals and in clinical situations. P-215: PERCEPTION OF ANTIOXIDANTS TO SCAVENGE FREE RADICALS IN HUMAN BODY Shila N Gevariya, Jalpa R Thakkar, HP Gajera, SV Patel, BA Golakiya Department of Biotechnology, Junagadh Agricultural University, Junagadh, Gujarat Free radical forms the core of modern theory of disease. It is paradox that oxygen, which is essential to life, may also contribute to hunam ageing and illness. When oxygen is metabolized or burned by the body cells, it forms byproduct called free radicals. Toxicity of oxygen is because of the processes that generate the free radocals in biological systems either as by-product or by-xenobiotic catabolism. These free radicals are unstable, highly reactive and energized molecules having unpaired electrons. Oxygen derived free radicals are Super oxide (O2-), Peroxyl (ROO-), Alkokyl (RO-) including reactive oxygen species Hydroxyl (OH-), Hydroperoxyl (HOO-), (ROS) produced in the body. These free radicals or ROS generated by oxidation is the main source of serious illness including many autoimmune disease and aging in the body. Antioxidant act as free radical terminator as it stop the free radical chain of oxidative reaction by contributing hydrogen from phenolic hydroxyl group and themselves forming stable free radical that do not initiate further oxidation. Thus antioxidant which naturally occurring in medicinal plants has potential protective functions against free radicals in mechanism of preventing much chronic and acute disease, which focused the attention of researcher. Poly phenol and flavonoids are usually recognized as the medicinal plant compounds which are responsible for the antioxidant activity. The present paper views a new approach of disease caused by oxidative stress and mechanism of action of antioxidants to scavange ROS. P-216: DNA FOOTPRINTING IN MEDICINAL PLANTS Zinkal A Katakpara, Nirvisha R Thumbar, HP Gajera, SV Patel, BA Golakiya Department of Biotechnology, College of Agriculture, Junagadh Agriculture university, Junagadh Footprinting is an essentially protection assay, in which cleavage of DNA is inhibited at discrete location by the sequence specific binding of ligand or protein. Footprinting provides simple, quick reasonably inexpensive method for assessing sequence specific interaction of ligand with DNA. Although the technique was developed in 1978 for studying interaction of DNA binding protein with their target site, and it has proved invaluable for determining the sequence specificity of many small ligands. In this technique, a DNA fragment of known sequence and length (restriction fragment), which has been selectively radiolaballed at one end of the one strand, is lightly digested by a suitable endonucleolytic enzyme in the presence and absence of drug under investigation. The cleavage agent is prevented from cutting around the drug binding site so that, when the product of the reaction are separated on denaturing polyacrylamide gel and exposed to auto radiography, the position of ligand or drug can be seen as gap in gel, otherwise continuous ladder of the band is observed. The application of DNA Footprinting in medicinal and pharmacological field is newly emerged by which we know the interaction of drug with specific sequence of DNA and inhibit the synthesis of harmful protein and also cell division in case of cancerous cell. It has been found that the extracts of medicinal plant or secondary metabolites like alkaloids which have medicinal values, affect the interaction of transcription factor of nuclear factor and specific DNA sequence. This technique is particularly useful in the treatment of tumor cell or malignant tissue. P-217: EXPRESSION AND FUNCTIONALITY CHLOROPLAST GENOME TO DERIVE VACCINES AND BIOPHARMACEUTICALS - A REVIEW Khyati Rathod, Shubhangi Oza, HP Gajera, SV Patel, BA Golakiya
Department of Biotechnology, Food Testing Laboratory, Junagadh Agricultural University, Junagadh Vaccines and therapeutic proteins are the great successes of modern medicine, which have been used for several decades to prevent diseases and eradicate them. Genetic engineering which altered chloroplast genome in plants offers several advantages, including high levels of transgene expression, transgene containment via maternal inheritance in a single transformation event. Mainly oral delivery is facilitated by hyperexpression of vaccine antigens against cholera, tetanus, anthrax, plague, or canine parvovirus in transgenic plant chloroplasts or non-green plastids (carrots, tomato) as well as the availability of antibiotic free selectable markers or the ability to excise selectable marker genes. Chloroplast also has the ability to process eukaryotic proteins including correct folding of subunits and formation of disulfide bonds. Functionality of chloroplast-derived vaccine antigens and therapeutic proteins has been demonstrated by several assays, including the macrophage lysis assay, GM1-ganglioside binding assay, protection of HeLA cells or human lung carcinoma cells against encephalomyocarditis virus, systemic immune response, protection against pathogen challenge, and growth or inhibition of cell cultures. Transgenic plant chloroplasts have the ability to express multiple genes in a single transformation event. The chloroplast expression system is an environmentally friendly approach. Therefore an attempt has been made to review economical production of the vaccines in plant using the chloroplast genome. P-218: LICHENS AS TREASURE CHESTS OF NATURAL ANTIMICROBICS Rajesh Dave, Rajendra Bambharolia, HP Gajera, SV Patel and BA Golakiya Department of Biotechnology, Food Testing Laboratory, Junagadh Agricultural University, Junagadh The utilization of lichen in medicine has been cited in different pharmacopoeias of the world. During the Middle Ages lichens figured prominently among the herbs used by medicinal practitioners. Lichens synthesize a wide range of primary (polysaccharides) and secondary organic compounds that show manifold bioactivities from nematocidal, antimicrobial, cytotoxic, antimutagenic and antiproliferative to immuno stimulatory effects. Out of 700 secondary metabolites so far known from lichens 550 are unique to them. The lichen extracts and their components have a distinguished antimicrobial activity. On the other hand, it is well known that microorganisms have well developed resistance to many antibiotics. This creates enormous problems in the treatment of infectious disease, and investigators therefore seek new antimicrobial substances from different sources so new sources of bioactive substances have been searched for, such as medicinal herbs, fungi and lichens. India being a mega diversity country exhibit rich diversity of different plant groups. The medicinal properties of higher group of plants are well known from the country. However, despite of the manifold medicinal uses of lower plants by traditional and ethnic groups the medicinal potential of these plants are not studied upto a greater extent. Thus, present paper views some antifungal activity of diferent foliose lichens against some common plant pathogenic fungi like Aspergillus flavus, Aspergillus fumigates, Alternaria alternate, Fusarium oxysporum, Fusarium solani etc. P-219: AN OVERVIEW: MOLECULAR FARMING FOR NEW DRUGS AND VACCINES Jayesh Kahodariya, Hitesh Ramani, HP Gajera, SV Patel, BA Golakiya Department of Biotechnology, Food Testing Laboratory, Junagadh Agricultural University, Junagadh Regardless of the availability of a vaccine or protein therapeutic, cost is a major factor for developing countries. The first recombinant plant-derived pharmaceutical protein (PDP) was human serum albumin, initially produced in 1990 in transgenic tobacco and potato plants. To avoid inadvertent entry into the food chain, the use of non-food crops such as tobacco is one option, but it is not prudent to exclude a priori major crop plants as hosts, particularly if the target molecule poses little or no risk to environmental or human health, such as recombinant antibodies. One of the most important driving factors has been yield improvement, as product yield has a significant impact on economic feasibility. Strategies to improve the recombinant protein yield in plants include the development of novel promoters, the improvement of protein stability and accumulation through the use of signals that target the protein to intracellular compartments, and the improvement of downstream processing technologies. Molecular farming is reaching the stage at which it could challenge established production technologies that use bacteria, yeast and cultured mammalian cells. Therefore an attempt has been made to review molecular farming potential for commercial drug development and production, but also the regulatory control, biosafety and political impacts of the technology, and its related intellectual property (IP) issues. P-220: TURMERIC IS THE ANTI-AGING, ANTI-OXIDANT, ANTI-INFLAMMATORY SUPER SPICE Amisha D Patel, Komal N Vaja, Vidushi N Vaja, HP Gajera, SV Patel, BA Golakiya Deaprtment of Biotechnology, Junagadh Agricultural Univesity, Junagadh, Gujarat Turmeric (Curcuma longa Linn.; syn.: Curcuma domestica, Curcuma aromatica) is a perennial from the Zingiberaceae family that is widely cultivated in the tropical regions of Asia, most extensively in India, and Latin America. Other names for turmeric include Indian saffron, turmeric root, and yellow root. Turmeric is warm, and bitter in taste. The applicable part of turmeric is the root, which is rich in potassium and iron. Chemical analysis of turmeric yields essential oils and fatty oils. An isolate from turmeric oil has been reported to have antifungal, antimutagenic, and antibacterial activity. Turmeric also contains curcuminoids altatone, bisdemethoxycurcumin, dimethoxycurcumin, diaryl heptanoids, and tumerone. Synthetic tumerone (turmerone) may act as an anticarcinogen. Throughout Asia, turmeric has been used for stomach problems, allergies, diarrhea, heartburn, wind, bloating, colic, flatulence and liver ailments. Curcumin, a polyphenol compound, is responsible for the yellow color of turmeric and is thought to be the most active pharmacological
agent. Natural curcumin, isolated from Turmeric, contains curcumin I (diferuloyl methane as the major constituent), as well as curcumin II (demethoxycurcumin) (6%) and curcumin III (bis-demethoxycurcumin) (0.3%). Turmeric may be standardized to contain approximately 95% curcuminoids per dose. The dried root of turmeric reportedly contains 4-8% curcumin, of which curcumin I is the most abundant, but may not be the most biologically active. Curcumin is insoluble in water and ether, but is soluble in ethanol, dimethylsulfoxide, and other organic solvents. Numerous animal and in vitro studies have demonstrated the ability of turmeric and its active component, curcumin, to suppress the growth of a variety of tumor cells. The postulated mechanisms for theseanticancer effects are multiple. Curcumin attacks multiple targets, providing the scientific basis for its effectiveness in many different diseases. Extensive research shows most diseases are caused by dysregulation of multiple signaling pathways--casting doubt on the effectiveness of monotherapy, which is limited to a single target. Studies show curcumin modulates numerous molecular targets, including: regulating several cytokines and fibroblast growth factor-2 (gene expression), growth-factor receptors including modulation of androgen receptors (protein kinases), transcription factors, pro-inflammatory enzymes (including supression of COX-2, 5LOX and iNOS and regulation of NF-B), modulation of cell-cycle-related gene expression, blocking the adhesion molecules, downregulating antiapoptic proteins and inhibiting multi-drug resistance. Product from Turmeric, BIOMORTM Curcumin provides curcumin blood levels up to 800% greater than other standardized 95% curcumin and 637% greater bioavailability than curcumin blended with piperine and lecithin. P-221: ANTI-ANXIETY POTENTIAL OF PASSIFLORA FOETIDA (AERIAL PARTS) Gagan Bhardwaj, Tajinder Singh*, Pradeep Kamboj, Ajudhia Nath Kalia Department of Pharmacognosy, ISF College of Pharmacy, Moga, Punjab Objective: Passiflora foetida L.; Passifloraceae (Stinking passion flower) is of South American origin, which has been spread to throughout tropical areas. Aerial parts of plant are reported to be rich in flavonoids and phenolic compounds. Literature claims that these phytoconstituents are responsible for anti-anxiety potential. Moreover, other species of Passiflora (P.incarnata and P.edulis) are pharmacologically reported to demonstrate a significant anti-anxiety activity. . Hence, this study was undertaken to biologically evaluate various extracts of Passiflora foetida (aerial parts) for its anti-anxiety potential in mice as till today no study has been undertaken. Method: The petroleum ether and methanol extracts were prepared by soxhlation and aqueous extract was prepared by triple maceration. Methanol extract was suspended in water and fractionated into chloroform, ethyl acetate and n-butanol fractions. All these fractions were studied for its in vivo anti-anxiety activity using elevated plus maze model. Results: Among all the extracts tested, maximum anxiolytic activity was observed with the methanol extract (300mg/kg; po) which was at par with that of standard drug diazepam. Furthermore, the ethyl acetate fraction (50 & 75mg/kg; po) of methanol extract significantly potentiated anxiolytic activity. Whereas, petroleum ether & aqueous extracts and chloroform & n-butanol fractions of methanol extract, were found to be devoid of anxiolytic activity. Flavonoids and phenolic compounds, as confirmed by phytochemical screening, in ethyl acetate fraction may be responsible for the anxiolytic activity due to their antioxidant potential. Conclusion: The result of the study concluded that P. foetida has significant in vivo anti-anxiety activity. Hence this plant can also be of medicinal interest. Keywords: Passiflora foetida P-222: ETHNO-HYPOGLYCEMIC GRASSES AND MANAGEMENT OF DM II Asha Arora and Vinita Paliwal Department of Botany and Biotech., B.N.P.G. Girls College, Udaipur, Rajasthan Traditional phyto-therapy is still practiced by local practitioners in different pouches of India. It hugs a vast array of plants including herbs, shrubs, trees, climbers, linnas etc. Present study compiles the ethno-medicinal survey of grasses utilized in diabetes mellitus II or during the tenure of hyperglycemia. These grasses were utilized as decoction or infusion and the mode of usage varied among different practitioners. Among enumeration bermuda grass (Cynodon dactylon), barley grass (Hordeum vulgare), wheat grass (Triticum aestivum) , fever grass( Cymbopogon citratus ), rosha grass(Cymbopogon martini), canary grass (Phalaris arundinacea), crab grass( Eleucine indica) and bamboo( Dendrocalamus strictus) are utilized utmost. Keywords: Ethno-hypoglycemic grasses, Diabetes mellitus II, wheat grass, fever grass P-223: DOCUMENTATION OF ETHNO-HEPATIC PLANTS FROM SOUTH-EAST RAJASTHAN Asha Arora, Jaishree Meena Dept. of Botany & Biotech., B.N.P.G.Girls College, Udaipur (Raj.) Present study deals with ethno-botanical documentation of 40 hepato-protective and hepato-curative plants used by different tribes of South-east Rajasthan such as Bhil, Meena, Garasia, Damor, Gamati, Parmar & Kalbelia . In application methods either decoction or infusion was preferred by local inhabitants over the other applying opts. Although the pathological differences about the liver s were not so clear among the practitioners but the generalized symptomlogy was akin to the clinical diagnosis. Among the reported plants Andrographis penculata, Citrullus colocynthus, Thuja occidentalis, Curcuma longa, Fumaria indica, Solanum nigrum, Eclipta alba and Phyllanthus amarus are reported to be most frequently utilized especially in the case of hepatic degeneration due to liquor consumption. Keywords: Ethno-hepatic plants, South-east Rajasthan, Hepatic degeneration P-224: IS AYURVEDA A COMPLIMENTARY SYSTEM OF MEDICINE?
Manojkumar Timbadiya, BR Patel, K Nishteswer Institute for Post Graduate Teaching and Research in Ayurveda, Gujarat Ayurveda Uni., Jamnagar Ayurvedic system is one of the world's most ancient systems of medicine which is well codified and built on the foundations of basic concepts like Panchamahabhoota doctrine, tridosha doctrine etc which are impervious to change. Yet in countries like US and elsewhere in the world it is given only the status of complementary medicine. National Centre for Complementary and Alternative Medicine defines CAM as a group of diverse medical and health care systems, practice Unlike other systems included under complementary medicine, Ayurveda is not just based on conventional use and personal experience but is a knowledge system evaluated by its own scientific method and thereby systematically documentated. It is a science of life which emphasises on the need to maintain health (physical, mental, social) and thus helps to produce complete well- being of an individual required to build up healthy society. The etiological factors responsible for the imbalance of somatic and psychological Dosha (humour) is delineated and methods used for the management is explained. To treat these vitiated humours, the correct therapy is adopted which while curing the disease does not provoke the manifestation of another disease. The materia medica of Ayurveda was developed extensively in terms of nomenclature, identification, mode of administration, pharmacodynamics and therapeutic effect of drugs from plant, animal and mineral origin. Drug formulations are designed according to disease, patient compliance, need of the body. Valiathan in his essay on "Towards Ayurvedic biology" highlights the fact that not one science is complete to tackle the epidemiological transition evolving in this century. The limitation of antibiotics in the management of infectious disease due to the bacterial resistance being a classical example, hence the need and focus has turned to Ayurveda, the traditional system which has a holistic approach. Hence this paper is an attempt to give a bird's view on all those aspects which will help in understanding whether Ayurveda is a just a complementary system or more. Keywords: Complimentary medicine, Ayurveda P-225: ANTI-INFLAMMATORY ACTIVITY OF AMAVATAVIDHAVANSA RASA Jalalpure Sunil, Mandavkar Yuvaraj, Kaushik Madan KLE University College of Pharmacy In the present study, Amavatavidhavansa rasa a herbomineral Ayurvedic formulation has been subjected to evaluate anti-inflammatory properties in carrageenan induced paw edema. Acute toxicity study was carried as per OECD guidelines 423 and drug was found safe at a dose of 2000 mg/kg b.w. The assessment of antiinflammatory activity was done by measuring the mean changes in volume of paw edema in ml on 1/2, 1st, 2nd, 3rd, and 4th h after induction and percentage inhibition of paw edema was calculated. Amavatavidhavansa rasa significantly reduced (P<0.01) the carrageenan induced paw edema as compared to standard drug (Diclofenac sodium 13.5 mg/kg, b.w., p.o.). The present investigation supports ethnopharmacological information regarding anti-inflammatory activity of Amavatavidhavansa rasa. Keywords: Amavatavidhavansa rasa, imflammation P-226: COMPARATIVE STUDY OF ELEMENTS PROFILE OF JUGLANS REGIA (WALNUT), PRUNUS DULCIS (ALMOND) AND ARACHIS HYPOGAEA (PEANUT) OF INDIA Zainab Bohra & Alphonso Priya Elphinstone College, Mumbai The objective of this study is to establish an elemental profile of Juglans regia, Prunus dulcis, Arachis hypogaea on a comparative analysis. It was found that the composition of walnut is quinines, oil, tannins, nuts contain essential fatty acids, including cis-linoleic and alfalinoleic, The kernels contain oil, mucilage, albumin, mineral, matter, cellulose & water. It is used for sore mouth and inflamed throat, it is also used to heal wounds, also used in skin diseases. The composition of almond is flavonoid oxidant, tocopherol, magnesium & calcium, copper,mono-saturated fatty acids. Its anti-oxidant prevent cancer. Almond also help in preventing cancer, heart disease. Almond is good for skin, it also prevent osteophorosis, it helps in sthrenting the bones, teeth & muscles, Prevent diabete,constipation,colon & rectal cancer. The composition of peanut is vitamin, protein, lipid, carbohydrate, fiber, mineral, vitamin, amino acids, salicylic acid, purines.These dry fruits play an important role in the diet and have important place in home remedial recipies. Keywords: Elemental Composition, Juglans regia, Prunus dulcis, Arachis hypogaea, remedial P-227: A NEW EMERGING TECHNIQUE FOR SEPARATION OF ACTIVE INGREDIENT FROM MEDICINAL PLANT A WAY FOR DRUG DEVELOPMENT: SEPBOX Kavita B. Joshi, Sacheen S. Raval, Mitali D. Vala, Jayeshkumar M. Sanghani, Tushar J. Antala Junagadh Agricultural University, Junagadh, Gujarat Still natural compounds play an increasingly important role in many areas of research and development. Their structural diversity is enhanced by the synthetic potential of combinatorial chemistry. Fractionation and separation of samples obtained from nature remain time-consuming, tedious and extremely expensive even though the assays for testing these samples have become faster and more cost-effective thanks to advanced high-throughput screening (HTS) processes. Today, sepbox (combination of high-performance liquid chromatography (HPLC) and solid-phase extraction (SPE)) is the standard technology used for separating compounds from natural resources. Fractionations and separation of samples obtained from nature remain very difficult. Automation of sample preparation therefore has great economic potential. The unique sepbox concept allows processing sample automatically and will make up to 30 times faster than by using a
conventional process. Various screening approaches are being developed to improve the ease with which natural products can be used in drug discovery campaigns, and data mining and virtual screening techniques are also being applied to databases of natural products. Keywords: High-throughput screening, natural compounds, separation, sepbox P-228: SPIRULLINA SUPPLEMENT AS MEDICINE Vidushi N. Vaja, Vinita D. Santoshi College of Computer, Science and I.T., Junagadh(Gujarat) Spirullina is a food that have an incredible array of health benefits that go well beyond just their nutrient value are considered 'super-foods.' The blue-green algae spirulina is one of the most primitive forms of life on the planet. This algae is known for its extraordinary survival adaptations. Protecting yourself in the event of a serious radiation event involves much more than simply loading up on potassium iodide and various other iodine supplements. While high levels of iodine do protect the thyroid and glandular systems from radiation poisoning Spirulina, literally the most nutritious food known, is an edible blue-green algae used all over the world for its nutritional and medicinal qualities. It is very common for people consuming Spirulina to experience a jump in energy and vitality. Energy is something that we all feel a little short on from time to time. It's a good thing that there are a myriad of natural energy boosters that we can eat, drink, or take as a supplement to help us be peak performers all day long. Many people don't know this, but spirulina is an outstanding dietary aid to help prevent (or reverse) anemia, treatment of brest cancer tumors ,HIV viruses and other viruses. Today's scientists are only beginning to grasp the incredible nutritional value of blue-green algae and spirulina, but these superfoods have a reputation that reaches far back into history. A form of blue-green algae was consumed regularly hundreds of years ago by Aztecs. It have been a strong proponent of astaxanthin, a remarkable nutrient sometimes called "King of the carotenoids!" Derived from microalgae (and also found in wild salmon), astaxanthin is on my list as one of the top three most important nutrients for human health. It has the ability to modulate immune functions and exhibits anti-inflammatory properties by inhibiting the release of histamine by mast cells.Multiple studies investigating the efficacy and the potential clinical applications of Spirulina in treating several diseases have been performed and a few randomized controlled trials and systematic reviews suggest that this alga may improve several symptoms and may even have an anticancer, antiviral and antiallergic effects. P-229: SIGNATURE ON THE SAFETY OF HERBAL DRUGS FOR HYPERTENSION Patel ZS, Faldu PR, Patel PM, Malaviya SV,Dadhaniya SK, Smt. R. B. Patel Mahila Pharmacy College, Atkot, Gujarat Cardiovascular diseases are the leading causes of morbidity and mortality all over the world, especially in developed and developing countries and affecting millions of individuals worldwide. Hypertension is one type of such progressive, complex and disabling cardiovascular condition. It is characterized by elevated systolic (> 140 mm Hg) and diastolic (> 90 mm Hg) blood pressure. Furthermore, persistent hypertension is one of the risk factor for stroke, myocardial infarction, heart failure and arterial aneurysm and is leading cause of chronic kidney failure. Many etiological factors such as age, smoking, obesity, oxidative stress, genetic mutation, smoking as well as even some disease condition and also drugs are critically involved in the pathogenesis of PD. However, till to date the exact cause of disease is still remain a mystery. The currently available pharmacological treatments are able to offer only symptomatic relief for patients; they do not cure or appear to alter the long term course of the underlying disease; also cannot completely ameliorate later-stage symptoms, nor can they address the ongoing secondary complications in body related to hypertension. So we should have to find other alternative therapy. In search of alternative treatment of hypertension, today world is moving toward the herbal options. There is a great deal of scientific evidence to suggest that the use of carefully chosen herbal remedies and dietary supplements can help to lower blood pressure, as well as to improve the overall functioning entire cardiovascular system. Hence in the present project, we have selected different plants, which are already reported to possess anti-hypertensive effect individually and act via different mechanism. Hence, the search for ideal anti-hypertensive drugs among these affords better protection and decrease the incidence of relapse. The pharmacists must take care in this aspect and try to make it into a suitable dosage form, which can improve compliance. Keywords: Hypertension, Anti-hypertensive drugs, Herbal drugs P-230: PHYTOCHEMICAL AND PHARMACOLOGICAL POTENTIAL OF STRYCHNOS POTATORUM: A REVIEW Karshan Bhetariya, Khyati Radadiya, Anu Patel, Roopal Tanna Department of Pharmaceutical Sciences, Saurashtra University, Rajkot In ayurveda many herbal plant parts have been employed for the treatment and management of various diseases since the beginning of human civilization. S. potatorum is an extensive genus of the family Strychnaceae (Loganiaceae). It is non toxic in comparison to S.nux-vomica. The present review comprises the ethnopharmacological, phytochemical and therapeutic potential of S. potatorum. The major databases such as Phytomedicine, Ethnopharmacology, Journal of Chemistry, Journal of Health Science, Journal of Medicine and Medical Sciences, Science Direct, Pub Med, and International Journal of Pharmacology were explored for the literature review. An exhaustive survey of literature revealed that saponins, flavonoids, alkaloids, glycoside, lignin, phenol, sterols, tannins constitute major classes of phytoconstituents of this plant. Pharmacological reports revealed that it is used as hepatoprotective, antioxidant, antiulcerogenic, nephroprotective,
antinociceptive, antipyretic, contraceptive, antidiarrhoeal, antidiabetic, diuretic, antiarthritic, antimicrobial, anti-inflammatory, antiplasmodial , cytotoxic effect. Seeds of S. potatorum also used as biosorption of cadmium and lead from aqueous solution and home water trement by direct filtration. S. potatorum seems to hold great potential for in-depth investigation for various biological activities, especially their effects on CNS disorder and kidney disease. Through this review, the s hope to attract the attention of natural product researchers throughout the world to focus on the unexplored potential of S. potatorum, and it may be useful in developing new formulations with more therapeutic value. Keywords: S. potatorum, Natural coagulant, Strychnaceae (Loganiaceae) P-231: HEPATOPROTECTIVE ACTIVITY EVALUATION OF HEDYCHIUM SPICATUM IN CARBON TETRACHLORIDE AND PARACETAMOL INTOXICATED RAT MODELS Shrinivas B. Bumrela and Suresh R. Naik Sinhgad Institute of Pharmaceutical Sciences, Lonavala, Pune (MS) The hepatorpotective activity of hydroalcoholic extract of rhizomes of Hedychium spicatum (HAEHS) in carbon tetrachloirde (CCl4) and Paracetamol (PCM) rat models was evaluated. The rats were administered (HAEHS) (250 and 500 mg/kg p.o.) and silymarin (100 mg/kg p.o.) daily for 5 days and received CCl4 1ml/kg i.p. (1:1 v/v CCl4:olive oil) on day 3 and 4, 30min after the silymarin and HAEHS. The rats received PCM (2 gm/kg p.o.) on day 5, 30min after the silymarin and HAEHS in PCM rat models. The hepatoprotective activity was assessed by determining serum biomarkers viz aspartate transaminase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) along with total bilirubin (TB) and total proteins (TP). Furthermore, various antioxidant paradigms viz. malondialdehyde (MDA) formation, superoxide dismutase (SOD), glutathione peroxidise (GSHPx) and catalase (CAT) were estimated in liver homogenates. In both CCl4 and PCM rat models, HAEHS treatment able to restore depleted antioxidant liver enzymes (SOD, GSHPx and CAT) and depleted the elevated serum biomarkers significantly respectively. In addition histoarchitecture of liver was also improved with the treatment of HAEHS. The protective activity of HAEHS (500 mg/kg p.o.) was comparable to that of silymarin (100 mg/kg p.o.). The hepatoprotective activity of HAEHS seems to be related to its antioxidant activity possibly through free radicals scavenging mechanism by activating antioxidant enzymes. The phytoconstituents (terpenoids, flavonoids, glycosides) of HAEHS may be responsible for hepatoprotective activity observed in the present study. Keywords: Hepatoprotective, Hedychium spicatum, antioxidant, phytoconstituent P-232: A REVIEW OF FICUS RELIGIOSA LINN. BASED ON ITS PHARMACOGNOSY, PHYTOCHEMISTRY AND PHARMACOLOGICAL USES Chirag Patel1, Harshit Patel1, Anu Patel1, Kapil Bhimani1, Vinit Thakkar2, Ashwin Dudharejiya1 1Dept of Pharm. Science, Saurashtra Uni., Rajkot, 2A.P.M.C.P.E.R., Himmatnagar Since ancient times, plants have been an exemplary source of medicine. Ayurveda and other Indian literature mention the use of plants in treatment of various human ailments. Ficus religiosa Linn is a large evergreen tree found in India Pakistan, Bangladesh, Burma, Ceylon, China and Thailand. It is popular in indigenous system of medicine like Ayurveda, Siddha, Unani and Homeopathy. In traditional system of medicine various plant parts such as stem bark, root bark, aerial root, leaves, fruits, seeds useful in diabetes, diarrhoea, leucorrhoea, menorrhagia, nervous disorders, ulcer, uterine tonic. The latex is good for neuralgia, inflammations and haemorrhages. Various plant parts are included in formulations used for menorrhagia, metrorrhagia, blood dysentery, bleeding piles, haematuria, haemorrhages and urinary troubles. The genus Ficus belonging to the family Moraceae constitutes an important group of trees with immense medicinal value. The present review of Ficus religiosa is therefore, an effort to give a detailed survey of the literature on its Pharmacognosy, phytochemistry, traditional and pharmacological uses. Keywords: Ficus religiosa, Peepal Tree, Pharamacognosy, Phytochemistry P-233: POLYPHENOL RICH BOERHAAVIA DIFFUSA L. EXTRACT AMELIORATES OXIDATIVE STRESS IN ANGIOTENSIN II INDUCED CARDIAC HYPERTROPHY IN H9c2 CELLS. A. Prathapan, K.G Raghu* CSIR-National Institute for Interdisciplinary Science and Technology (NIIST), Trivandrum, Kerala Cardiac hypertrophy is a major cause of morbidity and mortality among various cardiovascular diseases worldwide. The hypertrophic process is mediated, in part, by oxidative stress and reactive oxygen species (ROS) modulate signalling pathways involved in cardiac hypertrophy. The present study aims to investigate whether Boerhaavia diffusa, widely used medicinal plant had any protective effect on cardiac hypertrophy. Polyphenol rich Boerhaavia diffusa extract (BDE) was screened against angiontesin II induced cardiac hypertrophy in H9c2 cells. Cytotoxicity of BDE was evaluated using MTT assay. Angiotensin II treatment increased cell size, cell volume, protein content and LDH leakage while BDE treatment reverted all these changes near to normal. Angiotensn II induced oxidative stress in hypertrophy was confirmed by increased level of lipid peroxidation and decreased level of reduced glutathione (GSH) and antioxidant enzymes (catalase, superoxide dismutase). Intracellular generation of ROS was quantified using DCFH-DA as probe. BDE treatment decreased the level of lipid peroxidation, increased the activities of antioxidant enzymes and non enzymatic antioxidant, GSH. BDE also down regulated intracellular ROS generation in hypertrophied H9c2 cells. In addition, in vitro chemical assays showed that BDE inhibits angiotensin converting enzyme (ACE) and xanthine
oxidase in a dose dependant manner. The overall results suggest that BDE prevents the development of cardiac hypertrophy through an antioxidant mechanism via ameliorating oxidative stress. P-234: PHARMACOGNOSTICAL PROFILE OF DESMODIUM GANGETICUM DC. AND DESMODIUM TRIFLORUM DC. Amit Makwana, Bhavesh Vaghela, B. R. Patel I.P.G.T. & R.A., G.A.U., Jamnagar In Ancient times, the knowledge of identification of drug was based on observational science. Identification of the drugs and their utility was the main base for writing the Nighantus. And in this period much attention was paid towards the proper identification of plants not only on morphological basis but also organoleptic methods. In the current practice, the unskilled Herb collectors might be misguided or could not differentiate between various species and pass on the raw material to wholesellor because of which adulteration and substitution studies are necessary to be carried out in practice. Desmodium genus is the most important ingredient of Dashamoola in which Shaliparni comes under Laghupanchamoola. The Shaliparni Desmodium gangeticum DC. is an accepted source. Desmodium lexiflorum DC and Desmodium triflorum DC, etc. are the commonly using instead of Desmodium gangeticum DC. by vaidyas of Saurashtra region. The present study will be carried out to explore and compare the pharmacognostic characterstics of Desmodium gangeticum DC and Desmodium triflorum DC from the view of its substitution. Keywords: Desmodium gangeticumDC.,Desmodium triflorum DC., Pharmacognosy P-235: A REVIEW OF FICUS GLOMERATA LINN. BASED ON ITS PHARMACOGNOSY, PHYTOCHEMISTRY AND PHARMACOLOGICAL USES Khyati Patel1, Anu Patel2, Khyati Radadaiya2, Karshan Bhetariya2, Ashwin Dudharejiya2 1Shri Sarvajanik Pharmacy college, Mehasana; 2 Dept of Pharm. Science, Saurashtra Uni. Rajkot Today, there is a renewed interest in traditional medicine and an increasing demand for more drugs from plant sources. This revival of interest in plant-derived drugs is mainly due to the current widespread belief that "green medicine" is safe and more dependable than the costly synthetic drugs, many of which have adverse side effects and truth is plants have been the major source of drugs in Indian system of medicine and other ancient systems in the world. The genus Ficus belonging to the family Moraceae constitutes an important group of trees with immense medicinal value. Ficus racemosa Linn. is a moderate sized avenue tree found throughout India. It is popular in indigenous system of medicine like ayurveda, siddha, unani and homoeopathy. In the traditional system of medicine various plant parts such as bark, root, leaves, fruits and latex are used in dysentry, diarrohoea, diabeties, stomachache, piles and as carminative and astringent and also as antioxidant and anticancer agent. The present review is therefore, an effort to give a detailed study of Pharmacognosy, phytochemistry, traditional and pharmacological uses. Keywords: Ficus racemosa, Umbaro tree, Pharmacognosy , Phytochemistry P-236: ENHANCEMENT OF CURCUMIN ACTVITY BY COMPLEXATION WITH FERROUS ION Nilesh Gaikar, Renu P. Kolte, Sandhya Parameswaran Saraswathi Vidya Bhavan's College of Pharmacy, Sonarpada, Dombivli (E), Maharashtra Objective: The aim was to stabilize curcumin by its complexation with divalent ion Fe2+ and evaluate its pharmacology as compared to curcumin alone. Method: Curcumin was extracted from rhizomes of Curcuma longa with 95% alcohol followed by purification using column chromatography. The isolated curcumin was complexed with ferrous sulphate in a propylene glycol/ water system. The complex was characterized by FTIR, UV, TG-DTA and HPLC analysis. Both the complex and curcumin alone was evaluated for its anti-inflammatory activity by the carrageen rat paw method & antimicrobial property by the agar diffusion method. A preliminary pharmacokinetic study of the complex on experimental rats was monitored based on the blood levels of curcumin attained. Results: A yield of 98% was obtained for the ferrous-curcumin complex. A structure for the complex was proposed based on the TG-DTA and spectroscopic studies. The complex showed statistically significant anti-inflammatory & antimicrobial property at a dose of 200mg/kg bw i.p calculated equivalent to curcumin. The pharmacokinetic studies indicated about twofold increase in the peak plasma level of curcumin for the complex (129.269 µg within 5 hrs. when compared with curcumin alone (85.69 µg in 2hrs). Conclusion: Fe- curcumin complex was found to be stable and showed longer duration of action with high absorption as compared to curcumin. Keywords: Curcumin, Ferrous -Curcumin Complex P-237: EFFECT OF ABSCISSIC ACID, A PHYTO-HORMONE, IN FRUCTOSE-INDUCED INSULIN RESISTANT RATS Sheth D.B.1, Tirgar P.R. 2, Chavda J.R. 1, Desai T.R.2 1 B. K. Mody Govt. Pharmacy College, Rajkot; 2 School of Pharmacy, RK University, Rajkot Insulin resistance is a metabolic disorder that prevails worldwide and plays a role in pathophysiology of most common human diseases including type 2 diabetes mellitus, hypertension, obesity, dyslipidemia and coronary heart disease. Insulin resistance can be induced by fructose-rich diet in rats. One of most effective medications in treatment of insulin resistance has been thiazolidinediones (TZDs). TZDs are reported to have some troublesome adverse effects. Abscisic acid is a phytohormone with structural similarity to TZDs. We investigated effects of 2-cis, 4-trans abscisic acid on glucose, serum insulin levels, liver glycogen and muscle glycogen in wistar rats fed with high fructose. The animals were divided into 4 groups: normal control, disease
control (10% fructose in drinking water, 30 days), standard treated (Pioglitazone 10mg/kg p.o. with 10% fructose in drinking water, 30 days) and Abscisic acid (ABA) treated (1mg/kg p.o. with 10% fructose in drinking water, 30 days) group (n=6). Fructose significantly increased serum fasting glucose (p<0.01), serum insulin (p<0.05), FIRI (p<0.01), liver and muscle glycogen (p<0.01) as compared to control group. Standard as well as ABA treated group significantly reduced serum glucose (p<0.01), serum insulin (p<0.05), FIRI (p<0.01), liver and muscle glycogen (p<0.01) as compared to disease control group. Our results show that administration of abscisic acid may be advantageous for preservation of the functional efficiency of pancreatic cells, probably by improving insulin action via PPAR agonistic action and thereby preventing induction of insulin resistance. Keywords: Abscisic acid, Thiazolidinediones, Insulin resistance P-238: ASSESSMENT OF SOIL PROPERTIES & ELEMENTAL COMPOSITION OF SOIL OF AN ORCHARD LOCATED AT BICHOLIN-GOA Alphonso Priya ,Bernadette Alphonso & Aparna Saraf The Institute of Science, Department of Botany, Mumbai The area under study comprises of an orchard in Bicholim Taluka, measuring 25 acres of land having a variety of fruit growing tress which include Anacardium Species, Mangifera Indica, Bambusa Species, Tectonagrandis, Bombaxciba, Garcina Choisey & more. Soil is an important abiotic component that aids in providing nourishment to the vegetation. The study was aimed to analyze soil composition which included moisture content, organic content, pH, texture of soil & Elemental Composition qualitatively & quantitatively. The area which is under study is adjacent to a final dumpy mine, which is continuously causing detoriation of the soil & making it Infertile affecting the productivity of the fruit yield by the activities carried out by the company. The soil type is red alluvial soil having pH 7 & having a large number of elements which include Al, B, Ba, Ca, Cl, Co, Cr, Fe, K, La, Li, Mg, Mn, Na, P, S, Sr, V, Ti, Y, Yb, Zn, Zr, Mo, I, As which is found to be helpful for plant growth. The analysis was carried out on the final product also which showed the composition similar to that on the layer surface layer of the alluvial soil, which is due to the particles which are carried and deposited by the wind however the particles are also found to be deposited on the surface of the plant leaves which is also analyzed. Thus the study shows that due to the mining activity the over-all fruit yield has been decreased in the orchard. Keywords: Bicholim, Elemental composition, pH, Soil, Texture P-239: SIDDHA MEDICINAL PLANTS IN DERMATOLOGICAL DISORDERS N. J. Muthukumar, M. V. Mahadevan, R. S. Ramaswamy, K. Mankickavasakam National Institute of Siddha, Chennai Health is a state of complete physical, mental, moral and social well being. A healthy body is an apt place for a healthy mind to dwell. Traditional systems of India like Siddha; a healing science has their own potency to overcome the suffering and maintain the good health. Earlier medicine was practices as an art, but nowadays it is a harmonious blend of scientific practice. Siddha system of medicine, the great healing science of Tamil nadu is one among the antiquated traditional systems of medicine of India. The distinctive feature of Siddha from other systems of medicine is its holistic approach. This system was established by Siddhars, the eternal persons enriched with supernatural powers. Through their spiritual wisdom they have blessed us with the treasure of therapeutic measures like Kayakalpam, Yogam, Varmam, Ennumerous herbal and herbo-mineral medicines and paved effective solution for various ailments of physique and psyche of mankind. According to Siddha system of medicine, Health is defined as the state of physical psychological, social and spiritual component of a human being which has been given in Siddha Thirumoolar in his Thirumanthiram as; "One that cures physical ailment is medicine, One that cures psychological ailment is medicine, One that prevents ailment is medicine and One that bestows immortality is medicine". In Siddha system of medicine, the diseases are classified as 4448 types on the basis of Mukkutram theory. Among them, skin diseases are classified into eighteen. For the management of skin diseases many herbal and herbo-mineral formulations were mentioned in the ancient siddha literatures. This paper deals with the herbs which are employed for various skin disorders in siddha system of medicine. P-240: PHARMACOGNOSTICAL AND BIOLOGICAL EVALUATION OF ARGYREIA NERVOSA LEAVES FOR ANTIULCER ACTIVITY IN WISTAR RATS Jyoti Thakur I.S.F. College of Pharmacy, Moga (Pb) Objective: The leaves of Argyreia nervosa (AN) are widely used in ulcerative conditions in traditional system of medicine. Till date, no scientific work has been carried out to validate its traditional use. Therefore, in order to validate the traditional claim, the present investigation was designed to study the pharmacognostical characters of AN and to evaluate its antiulcer effects. Method: Morphological, microscopical and powder characteristics were determined. The 50% methanolic extract (AN-Me) was prepared and fractionated into pet. ether (PE-F), ethyl acetate (EA-F), n-butanol (nB-F) and aqueous (A-F) fractions. The antiulcer activity of all the above extracts of AN leaves was evaluated using Indomethacin and Pylorus ligated induced antiulcer models. Results: Leaf was dorsiventral or bifacial, single layered; T.S. showed thin cuticle followed by 3-4 layers of epidermal cells; covering trichomes present on upper and lower epidermis, vascular bundles consisting of phloem and xylem vessels. Powdered microscopy showed simple, tricellular, warty covering trichomes; vessels with boarded pits and xylem vessels with spiral thickenings. Phytochemical screening of AN-Me, EA-F, nB-F and
A-F revealed the presence of carbohydrates, phenolic compounds, flavonoids, steroids and triterpenoids. EA-F exhibited a dose dependent antiulcer activity with maximum effect at 36.48 mg/kg in both the models. The results was further supported by histopathological studies. Conclusion: Morphological, microscopical and powder characteristics confirmed that the plant belongs to Convolvulaceae family. Further, investigations clearly indicate the antiulcer effect of EA-F of AN and validating its traditional use for anti-ulcer property. Further work is in progress. Keywords: Argyreia nervosa, anti ulcer P-241: MICROPROPAGATION OF SAFED MUSLI (CHLOROPHYTUM BORIVILIANUM) & STUDIES ON GENETIC INTEGRITY OF MICROPROPAGATED PLANTS THROUGH RAPD Rashmin Dhingani, Rukamsingh Tomar, Manoj Parakhia, Bipin Malaviya, Sangamitra Samantray & B. A. Golakiya Junagadh Agricultural University, Junagadh; National Research Centre for Medicinal plants, Boriavi Medicinal plants are earliest known health care products that have been used by the mankind. Over three quarters of the world population rely on the use of traditional medicines for their primary health care needs. Chlorophytum borivilianum belonging to family Liliaceae, popularly known, as safed musli is an important medicinal plant. Its peeled and dried tubers are considered as a wonder drug in Indian systems of medicines due to its aphrodisiac and natural tonic properties because of great therapeutic importance. Large-scale collections from the natural stands and destructive nature of harvesting, shy flowering with low seed formation, poor seed germination and low tuber multiplication ratio raise the concern about the possible extinction of the species and simultaneously provide justification for the development of In vitro propagation. Rapid micropropagation of Chlorophytum borivilianum through In vitro culture technique was studied by manipulation of hormone regime. Rapid shoot multiplication was achieved on half MS media containing 3.0 mg/l BA + 0.1 mg/l NAA and 150 mg/l Ads after 9th subculture. It was observed that half strength MS nutrients and 3% sucrose supplemented with 3.0 mg/l BA and 0.1 mg/l NAA under 16-h photoperiod with 4500 lux light intensity and 40% relative humidity favored high frequency shoot multiplication. The elongated shoots were rooted on media containing half strength MS basal salts supplemented with 0.1 mg/l IAA and 1% sucrose. The same media with 1% glucose helped in tuberous root formation. The plants in the field with tuberous roots survive (99%) as compared to plantlets with fibrous root. Evaluation of genetic stability of the micropropagated plants was made using RAPD markers. The results were scored as the pattern of bands obtained from the In vitro grown plant which was compared with the mother plants. Out of 32 different decamers tested, seven primers produced good amplification products that were monomorphic across all the micropropagated plants; no polymorphism was detected. Keywords: Chlorophytum borivilianum, micropropagation, genetic integrity P-242: ETHNOPHARMACOLOGICAL STUDIES ON MYRICA ESCULENTA BUCH-HAM. STEM BARK A NOVEL ANTIPYRETIC HERBAL DRUG USED BY THE NAGA TRIBES OF MANIPUR C.L. Ringmichon, A. P. Dixit, Late. Shraddha N. Shimpi & Bindu Gopalkrishnan Department Of Botany, K. V. Pendharkar College, Dombivli (E), Maharashta Fever is a common ailment occurring in many of us. Allopathic medicines like Crocine, Paracetamol, Nimuslide etc. are used to bring down the body temperature but these medicines have side effects. Hence the search for herbal cure was felt necessary. Myrica esculenta BuchHam. Stem bark from family Myricaeae is studied for its antipyretic activity, since the Naga tribes of Manipur depend on this stem bark for curing fever. To prove it scientifically the aqueous extract of Myrica esculenta stem bark at a dose of 300, 400 and 500 mg/kg body weight were tested on brewers yeast induced pyrexia Wistar albino rats. It was compared with the standard drug paracetamol fed rats. The pyrexia in rats fed with Myrica esculenta was significantly compared to that of paracetamol fed rats. Thus the results indicate that the said plant extract possess potential antipyretic properties as claimed by the Naga folk. Keywords: Antipyretic, Myrica esculenta, Myricaceae P-243: MEDICINAL PLANTS AND CANCER CHEMOPREVENTION B. J. Malviya, R. S. Dhingani, R. S. Tomar, M. V. Parakhiya Department of Biotechnology, Junagadh Agricultural University, Junagadh Cancer is the second leading cause of death worldwide. Although great advancements have been made in the treatment and control of cancer progression, significant deficiencies and room for improvement remain. A number of undesired side effects sometimes occur during chemotherapy. Natural therapies, such as the use of plant-derived products in cancer treatment, may reduce adverse side effects. Currently, a few plant products are being used to treat cancer. However, a myriad of many plant products exist that have shown very promising anti-cancer properties In vitro, but have yet to be evaluated in humans. Further study is required to determine the efficacy of these plant products in treating cancers in humans. This review will focus on the various plantderived chemical compounds that have, in recent years, shown promise as anticancer agents and will outline their potential mechanism of action. Keywords: cancer, chemoprevention, Natural therapy, anti-cancer
P-244: ARABINOGALACTAN: A THERAPEUTIC POTENTIAL BIO MOLECULE Mavani NK, Pathak IS, Bhatt PR, Sheth NR Department of Pharmaceutical Sciences, Saurashtra University, Rajkot (Gujarat) Arabinogalactans (AGs), a class of polysaccharides, have been reported to be present in a wide range of plant taxa. AGs present in the rhizome of Curcuma longa and other several herbaceous plants, such as Baptisia tinctoria, Echinacea purpurea, Echinacea pallid, and Angelica acutiloba. AG present in the rhizome of Curcuma longa, has the ability to activate reticuloendothelial cells; such stimulated cells with enhanced phagocytic activity. Thus, AG acts as a strong activator for two cell types involved in the immune system, namely macrophages and NK cells. In addition, AG has the ability to block the metastasis of liver tumor cells. AG has been shown to reduce tumor cell colonization and increase the survival time of patients suffering from cancers of the spleen, liver, and colon. AG has been used with encouraging results in combating influenza and cold as well as in lung and ear infections. AG also decreases serum lipids in hyperlipidemic individuals, and modulates serum glucose. Various characteristics of AG shows promise of a novel fiber as it is a soluble dietary fiber that is easily incorporated into foods and is fermented in the gut with physiological changes that are beneficial to health, and have other immunological functions. Prebiotics including AG are now being used to treat intestinal disordersand inflammatory bowel diseases. Keywords: Arabinogalactan, Curcuma longa, Baptisia tinctoria, Echinacea purpurea P-245: HEPATOPROTECTIVE ACTIVITY OF ETHANOL EXTRACT OF BERBERIS CORIACEAE IN CCl4 TREATED MALE RATS Rajendra Jangde University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur (C.G.) To evaluate the hepatoprotective effect of the ethanol leaves extract of Berberis coriaceae leaves in male wistar albino rats treated with Carbon tetrachloride. Liver damage was studied by assessing parameters such as serum glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, alkaline phosphatase, acid phosphatase and bilirubin in serum. The effect of administration of ethanol extract at dose of 100 mg/kg and 200 mg/kg on the above parameter was further investigated. Histopathological study of the liver in experimental animals was also undertaken. Results of this study revealed that the suspension of ethanol extract showed significant hepatoprotective activity (p<0.01) by reducing the levels of the biochemical parameters in experimentals animals. The results were supported by histopathalogical studies of liver samples showing regeneration of hepatocytes by the extract. The ethanol extract of both doses afforded significant protection against CCL4 induced hepatocellular injury. Keywords: Carbon tetrachloride, Marker enzymes, Berberis coriaceae P-246: POLYHERBAL FORMULATION PROTECTIVE AGAINST ETHYLENE GLYCOL INDUCED UROLITHIASIS IN RATS Vrushali V. Patel, Jitendra D. Vaghasiya, Vandana B. Patel, Ghanshyam Patel Babaria Institute of Pharmacy, Vadodara, Gujarat Present study was aimed to investigate the effect of Polyherbal formulation (PHF) (Ural) against ethylene glycol induced urolithiasis in albino wistar rats. Urolithiasis was induced in rats by administering 0.75% ethylene glycol in drinking water for 28 days and was manifested by high urinary calcium, phosphate, oxalate, and high serum creatinine contents. Simultaneous administration of PHF orally for 28 days along with ethylene glycol (0.75% v/v) significantly (P < 0.05) reduced urinary calcium, phosphate, oxalate and elevated serum creatinine level. PHF also increased urinary volume thereby reducing the tendency for crystallization. The histopathological studies demonstrated degenerated glomeruli, necrotic tubule and inflammatory cells from animals treated with ethylene glycol which were preserved in PHF treated rats confirmed the protective effect. These observations conclude that PHF is effective against ethylene glycol induced urorolithiasis. Keywords: Urolithiasis, Ethylene Glycol, Polyherbal Formulation, Calcium Oxalate P-247: GLOBAL REGULATIONS AND QUALITY STANDARDS FOR VALIDATION OF ANALYTICAL METHODS Bhargav Shilu Dept. of Pharmaceutical Science, Sau.Uni., Rajkot Regulations and quality principles that have an impact on analytical laboratories need analytical methods to be validated. The guideline may use the word "validation" directly, list specific parameters, or involve validation with statements such as: "analytical methods should be suitable for their proposed use". This piece of writing reviews the most important policy, quality standards and official guidelines. The scope of guidelines for validation requirements provided by different organizations varies broadly, but the objective of validation is always to accomplish valid analytical test outcome. This is significant to ensure the quality and safety of products that are calculated using the analytical method. The regulation and quality standard for analytical method validation mainly expressed here according to United States Food and Drug Administration (FDA), Pharmaceutical Inspection Cooperation Scheme (PIC/S) and Europe, International Conference for Harmonization (ICH), Unites States Pharmacopeia (USP) and ISO/IEC 17025. Keywords: quality standards, method validation, method development P-248: EVALUATION OF ANTIUROLITHIATIC ACTIVITY OF AQUEOUS EXTRACT OF ABUTILON INDICUM (L.) SWEET LEAVES
Kore K. J.*, Shete R. V., Gurav V. R., Todkari N. T. Rajgad Dnyanpeeth's College of Pharmacy, Bhor, Pune Renal lithiasis, also called nephrolithiasis or urolithiasis, is the clinical condition caused by the formation and movement of stones within the urinary tract. Nephrolithiasis is largely a recurrent disease with a relapse rate of 50% in 510 years and 75% in 20 years. The results of antiurolithiatic study indicated that aqueous extract of Abutilon indicum leaves (500 and 1000 mg/kg) prevented the growth of urinary stone in ethylene glycol with ammonium chloride as well as in preventive and curative treatment groups of Cystone (750mg/kg; p.o.) with CPD induced urolithiasis rat models. Antiurolithiatic effects of aqueous extract of Abutilon indicum L. leaves may be due to presence of tannins, flavonoids and saponins which may act individually or in combination. Keywords: Nephrolithiasis, Urolithiasis P-249: MEDICINAL PLANTS AS A SOURCE OF ANTICANCER DRUGS: A REVIEW Pathak DP1, Bhatt PR*1, Savjiyani JV2, Sheth NR1 1Dept of Pharm. Sci., Sau.Uni., Rajkot; 2Shree Leuva Patel Trust Pharmacy Mahila College, Amreli Cancer is a major cause of death and the number of new cases, as well as the number of individuals living with cancer, is expanding continuously. Due to the enormous propensity of plants that synthesize mixtures of structurally diverse bioactive compounds, the plant kingdom is potentially a very diverse source of chemical constituents with tumor cytotoxic activity. Plant-derived compounds have played an important role in the development of several clinically useful anti-cancer agents. These include vinblastine, vincristine, camptothecin derivatives, topotecan and irinotecan, etoposide, derived from epipodophyllotoxin, and paclitaxel (taxol®). Though significant progress has been made towards the characterization of isolated compounds and their structure-related activities, the complex composition of plant extracts, along with the lack of reproducibility of activity and the synergy between different, even unidentified, components of an extract, prohibits the full utilization of plants in pharmaceutical research. Keywords: Anticancer plants, Herbal drugs, Medicinal plants, Cancer research P-250: ASPARAGUS RACEMOSUS-A PROSPECTIVE MEDICINAL PLANT AS ADDITIONAL SOURCE OF INCOME FOR MARGINAL FARMERS Bindiya Prakash, Manoj Chouraisya and Padma Harshan Society for Human Welfare and Environmental Furtherance (SHWEF),Bhopal Asparagus racemosus (Shatavari) is an important medicinal plant of tropical and subtropical India. Its medicinal usage has been reported in the Indian and British Pharmacopoeias and in traditional systems of medicine such as Ayurveda, Unani and Siddha. The roots are used in Ayurvedic medicine, following a regimen of processing and drying. It is generally used as a uterine tonic, as a galactogogue (to improve breast milk), in hyperacidity and as a general health tonic. The main Ayurvedic rejuvenative tonic for females. It is however, used for sexual debility and infertility inboth sexes. It is also used for menopausal symptoms .It's soothing and antispasmodic diuretic qualities are already known. In Ayurveda, it has been described as a rasayana herb and has been used extensively as an adaptogen to increase the non-specific resistance of organisms against a variety of stresses. Besides use in the treatment of diarrhoea and dysentery, the plant also has antioxidant, immunostimulant, anti-dyspepsia and antitussive effects. Due to its multiple uses, the demand for Asparagus racemosus is constantly on the rise. Destructive harvesting, combined with habitat destruction, and deforestation, the plant is now considered 'endangered' in its natural habitat. The average yield of Shatavari is reported to about 2607 gms fresh weight per plant after 40 months age. The roots come to maturity in about 12-14 months after planting depending upon the soil and climatic conditions. A single plant may yield about 500 to 600 g of fresh root. On an average, 12,000 to 14,000 kg of fresh roots can be harvested from one hectare area which on drying may yield about 1000 to 1200 kg of dried roots. Realizing the importance of this plant and now in the endangered status we deemed it appropriate for cultivation as off-farm wage-earning alternatives with other labor-intensive crops that occupy the entire family-based labor force and provide an additional acceptable income for the marginal farm household. Keywords: Shatavari, galactogogue, fresh roots, additional acceptable income P-251: ANALGESIC ACTIVITY OF ESSENTIAL OIL EXTRACTED FROM COLEUS AROMATICUS LINN. LEAVES AGRAWAL A, DEHARIYA B, SHARMA HK, VERMA CJ Patel College of Pharmacy, Ratibad, Bhopal Coleus aromaticus (Lamiaceae) essential oil useful in bronchitis, asthma, cold, chronic cough, epilepsy, diarrhea. Coleus aromaticus is commonly considered as a medicinal plant, useful for relieving headache, colic, flatulence, rheumatism, otalgia (ear aches). The aim of the present study was to evaluate the analgesic activity of an essential oil extracted from Coleus aromaticus leaves in mice. Essential oil was extracted by subjecting weighed amount of leaf to hydrodistillation by using Clevenger's apparatus. Finally the oil was collected. The collected oil was used for analgesic effect. Analgesic activity of essential oils at different dose levels was studied on 20-30gm Swiss albino mice. The reaction time at 15, 30, 60, 90, 120 minutes were measured. The pain sensitivity was assessed by the acetic acid induced writhing models in mice. Thirty mice (20-30 gm) were randomly divided into 6 groups. Group I was given distilled water 1 mL as negative control, group II Paracetamol 65 mg/kg BW as positive control, and group III-VI received an Essential oil of coleus aromaticus leaves in 4 doses, i.e. 50 mg/kgBW, 100mg/ kgBW, 250mg/kgBW, and 400mg/kgBW, respectively. All interventions were administered as a single dose by oral route on a given day. Acetic acid 0.6% (w/v) was used as a pain inductor. Analgesic
activity was measured by counting the percentage of writhing movements as a measure of the analgesic effect produced by each intervention. Data were analyzed with one-way ANOVAs to compare analgesic activity between treatment groups. In the acetic acid-induced writhing test, the essential oil of coleus aromaticus leaves at the 200 and 400 mg/kg dose level showed 69.9 % and 72.5 % inhibition of writhing, respectively. These experimental results suggest that essential oil (200 and 400 mg/kg, p.o) produced analgesic effect in mice and may be a candidate for the development of pharmacological agents used in the pain management. Keywords: Coleus aromaticus, Analgesic activity, acetic acid induced writhing model P-252: IN SILICO ANALYSIS TO EVALUATE THE DRUGGABILITY OF PIPER NIGRUM Shibi I.G., Sajeev R., Neena Haridas, Mumthas R.B. Department of Chemistry, Sree Narayana College, Thiruvananthapuram, Kerala Piper nigrum (black pepper) is considered as an important healing spice in ayurveda. In the present study, in silico tools are applied to 23 chemicals in piper nigrum, for understanding their biological activities and druggability. The biological activities are predicted by Pass Inet and the druggability of the compounds is checked by the QikProp of Schrodinger Maestro. The physically significant descriptors and pharmaceutically relevant properties of the selected compounds are predicted. Similarity search by BIDD, identified the drugs which are very similar to some of the bioactive components of piper nigrum. Sesquisabinene or 5-(6methylhept-5-en-2-yl)-2-methylidenebicyclo[3.1.0]hexane showed very high similarity with 17 drugs with Tanimoto coefficient value higher than 0.9500. Structural feature studies carried out by ChemAxon gave the logP, charge, polarizability, orbital electronegativity, topology, polar Surface area, molecular surface area (3D), hydrogen bond donor-acceptor, Huckel analysis, structural framework etc. The protein target information of Calcidiol, the drug with maximum similarity with Sesquisabinene, is collected. The drug action of Sesquisabinene and its thirteen structural derivatives towards those four protein targets namely 1DB1.pdb, 1IE8.pdb, 1IE9.pdb and 1S19.pdb are understood by docking studies. The results of these in silico studies indicate that, in contrast to several other compounds in piper nigrum, Sesquisabinene and its derivatives exhibit good activities which have to be confirmed by both pharmacological and clinical studies. In silico approaches can help in identifying better drug candidates that are safe, besides cutting down the high costs. Keywords: Piper nigrum, druggability, computer aided drug design, sequisabinene P-253: EXENATIDE PROTECTS ISOPROTERENOL INDUCED LEFT VENTRICULAR HYPERTROPHY IN RATS Ashokji B. Thakor, Jitendra D. Vaghasiya, Arindam Paul, S. S. Pancholi Babaria Institute of Pharmacy, Varnama-Vadodara, Gujarat Background/Aim: Exenatide, as GLP-1 receptor mimeticincreases cAMP and promote the activity of phosphoinositide 3-kinase, and this mechanism reported to prevention against hypertrophy. So we hypothesized exenatide could protect against left ventricular hypertrophy (LVH). Methods and Results: LVH in rat was evoked by repeated administration of isoproterenol 5 mg/kg s.c. for 7 days. Systolic blood pressure, diastolic blood pressure, mean blood pressure, ECG and heart rate were measured by student physiograph. Left ventricular mass were measured to determine cardiac hypertrophy index, which was significantly lower in exenatide treated rats. We observed elevation of ST- segment indicated successful LVH in LVH control rats. Exenatide treated rats that underwent LVH demonstrated significant (P < 0.05) normalization of hemodynamic parameters. The infarct sizes were significantly (P < 0.05) reduced in exenatide treated rats compared to non treated rats. Exenatide treated rats demonstrated significant (P < 0.01) restoration of membrane bound enzymes like Na+ - K+ ATPase, Ca2+ - ATPase and Mg2+ - ATPase compared to LVH control rats suggested stabilization of membrane bound enzymes which may reduce Ca2+ overload. Lipid peroxidation, myeloperoxidase activity and nitric oxide level in cardiac tissue were significantly (P < 0.05) decreased in exenatide treated rats compared to LVH control rats. Antioxidant enzymes like glutathione, superoxide dismutase and catalase were significantly (P < 0.01) increased after left ventricular hypertrophy in exenatide treated rats compared to non treated rats. Conclusions: In conclusions, exenatide attenuated left ventricular hypertrophy and the prevention being shown to achieve via reducing Ca2+ overload in cardiomyocyte. Keywords: Exenatide, left ventricular hypertrophy, infarct size, calcium overload P-254: ROLE OF PUERARIA TUBEROSA DC EXTRACT ON ANDROGENESIS AND SEXUAL BEHAVIOR VIA FSH LH CASCADE Nagendra Singh Chauhan, Vikas Sharma, Mayank Thakur, Alexandra Christine Helena Frankland Sawaya, V. K. Dixit Drug testing Laboratory avam Anusandhan Kendra, Raipur; Department of Pharmaceutical Sciences, H. S. Gour Vishwavidyalaya, Sagar Pueraria tuberosa (PT) is described in Ayurveda as vajikaran rasayan. The aim of this study was to investigate the effects of ethanolic extract of PT on sexual behaviour and the mechanism underlying the increase of androgenic activity; that is the effect of ethanolic extract on change in sexual behaviour, weight of sexual organs, histology of testis and epididymis, level of fructose and sperm count and measurement of hormones regulating androgenic and sexual behavior i.e. follicle stimulating hormone (FSH), luetenizing hormone (LH) and testosterone (T). Male albino rats were divided into four groups of six animals each; the control group 1 (2% acacia solution), PT treated group 2 (50 mg/Kg), PT treated group 3 (100mg/Kg) and PT treated group 4 (150mg/Kg). Sexual behavior of male rats of in the presence of a female rat, in a special cage was recorded. The
treated groups were evaluated for sexual parameters. The extract was characterized using LC-MS. The effect of treatment on anabolic and weight of secondary sexual organs was determined on day 0 and day 28. The histological changes in section of testis and epididymis post treatment were observed. Sperm count in epididymis and fructose content in seminal vesicles were also measured. Levels of hormones like FSH, LH and T were determined. A dose dependent increase in sexual behaviors was evidenced by increase mount frequency and decrease in mount latency, intromission latency, post ejaculation latency in the animals of extract treated groups. Increase in testis weight was recorded in PT with the most effective dose being 150 mg/Kg bw. At the highest dose PT also affects the hormones level. The four compounds viz Puerarin, Daidzein, Biochanin-A and formonetin was identified in ethanolic extract using LC-MS. It concluded that PT extract possess androgenic effect and it significantly increased the sexual behaviour and hormones level. Keywords: Pueraria tuberosa, sexual behaviour, testosterone, FSH, LH, reproductivity P-255: A PILOT STUDY TO COMBAT THE SIDE EFFECT OF RADIATION WITH ASHWAGANDHA IN ADVANCED CASE OF HEAD & NECK CANCER & CERVICAL CARCINOMA Ghodasara VR*1, Bangoriya PN1, Joshi BG2, Sheth NR1 1 Dept of Pharm. Sciences, Saurashtra Uni.; 2 Atharva Ayurveda & cancer research center, Rajkot Head & neck cancer is a leading cause of morbidity & mortality in India. Among them oral cancer & cervical cancer constitute 25-30 % of all cancer in various hospitals in India. Surviving and living longer is not an ultimate aim for most of the cancer patients. They need to be made comfortable to carry out everyday activity without physical fuse and emotional fissure therefore, they must be rehabilitated. Radiation therapy is frequently used as primary or curative mode of therapy in so many cancers. In radiotherapy x-ray particles are aimed at the tumor to cause damage in the cells of the tumor. However, because of the location of the tumor, it is impossible to avoid some damage to the normal cells that are in the path of radiation beam. The main property of Aswagandha is a natural Anti-oxidant, which helps to increase the molecular effect of radiation. Thus this drug acted both directly on cancer cells and by making them more sensitive to Radiotherapy. Aswagandha helps to decrease the amount of Glutathione in tumor cells after which radiation therapy become more effective and reduces the discomfort and pain and decrease side effect to normal cell during radiotherapy period. Patient tolerates radiotherapy in better manner. We have randomized 33 patients. Out of 33 patients 28 were from advanced head and neck cancer and 5 patients were of Ca. Cervix iiiB. Each patient gives Aswagandha (WITHANIA SOMNIFERA) mool extract in dose of 5 Gm. per day with milk before external radiation (conventional RT.).Out of 33 patients 14 patients (42.42%) developed mild reaction, 17 patients (51.51%) developed moderate reaction. Only two patient (6.06%) developed severe reaction. Present prospective study compared radiotherapy plus herbal therapy in previously untreated oral cancer and cervical cancer. Most important aims to provide and agent which combact the side effect of the radiotherapy by this concomitant radio-herbal protocol. P-256: EVALUATION OF ANTI-INFLAMMATORY ACTIVITY OF SOME LIBYAN MEDICINAL PLANTS IN EXPERIMENTAL ANIMALS JAMAL ELMEZOGI1, ABDULMOTTALEB ZETRINI2, GHAZALA BEN-HUSSEIN1, MASOUD ANWAIR1, ABDUL GBAJ1,2, MABROUKA EL-ASHHEB3, LUTFUN NAHAR4 AND SATYAJIT D. SARKER5* 1Faculty of Pharmacy, Tripoli University, Libya; 2National Center for Medical Research, Zawia, Libya; 3Faculty of Medicine, Tripoli University, Libya; 4Leicester School of Pharmacy, De Montfort University, Leicester, UK; 5Department of Pharmacy, University of Wolverhampton, UK Ballota pseudodictamnus (L.) Benth. (Lamiaceae), Salvia fruticosa Mill. (Lamiaceae) and Thapsia garganica L. (Apiaceae) are three well known medicinal plants from the Libyan flora, which have long been used for the treatment of inflammations. The aim of the present study was to investigate, for the first time, the antiinflammatory property of the extracts of the methanol (MeOH) extracts of the aerial parts of these plants. Shade-dried and ground aerial parts of B. pseudodictamnus, S. fruticosa) and T. garganica were Soxhletextracted with MeOH. The extracts were concentrated by evaporation under reduced pressure at 40°C. The antiinflammatory activity of the extracts was evaluated using the carrageenan-induced mice paw oedema model. The administration of the extracts at the dose of 500 mg/kg body weight produced statistically significant inhibition (p < 0.05) of oedema within 3 h of carrageenan administration. The results demonstrated significant anti-inflammatory properties of the test extracts. Among the extracts, S. fruticosa extract exhibited the most significant inhibition of inflammation after 3 h (62.1%). Thus, S. fruticosa could be a potential source for the discovery and development of newer anti-inflammatory `leads' for drug development. The anti-inflammatory activity of B. pseudodictamnus and S. fruticosa could be assumed to be related to high levels of phenolic compounds, e.g., flavonoids, present in these plants. Keywords: Ballota pseudodictamnus, Salvia fruticosa, Thapsia garganica, carrageenan P-257: PREVENTIONAL APPROACH FOR CANCER BY USE OF NUTRACEUTICALS Parekh Priyam1, Divyang Patel 1Department of Quality Assurance, Shree Devi College of Pharmacy, Mangalore, Karnataka In the United States, cancer is responsible for 25% of all deaths with 30% of these from lung cancer. The most commonly occurring cancer in men is prostate cancer and in women is breast cancer.The variation in occurance of cancer is mainly due to environmental factors and lifestyle, rather than genetic factors, as immigrants from low-incidence countries to high-incidence areas acquire the cancer pattern of the host country within a relatively short period
of time, for example within in a single generation, and genetic changes would take longer than one generation to show an effect. Although screening was shown to identify cancer cases at an earlier stage of the disease, it failed to reduce the total incidence of cancer. In addition, concerns over the safety and efficacy of conventional cancer therapies have stimulated the search for new alternatives. It is now widely accepted that the best way to limit the incidence of cancer is by prevention. The complexity and diversity surrounding each type of cancer, and the resulting morbidity and often mortality, has led researchers to explore the use of a number of nutraceuticals. Soy, green tea, lycopene, flaxseed, polyunsaturated fatty acids (PUFAs), coenzyme Q10 (Co Q10), melatonin and conjugated linoleic acid (CLA) have all been proposed as preventive treatment for a range of cancers, including leukaemia, lymphoma, prostate, breast, lung and colon. Keywords: Cancer, Nutraceuticals, Prevention. P-258: ANTICARIOGENIC AND HEMOLYTIC ACTIVITY OF SELECTED SEED PROTEIN EXTRACTS AND THEIR POTENTIAL USE IN TOOTH INFECTION Pankit P. Shah, Kalpesh B. Ishnava ARIBAS, Sardar Patel University, Vallabh Vidyanagar, Gujarat The antimicrobial sensitivity assay of crude plant seed protein extracts against tooth decaying organisms was carried out. The proteins from seeds of 12 different plants were extracted and then used for antimicrobial assay against 6 different organisms. The extraction was carried out in 10mM of sodium phosphate buffer (pH 7.0). The antimicrobial assay results in activity of Mimusops elengi against Staphylococcus aureus and Streptococcus pyogenes. A little activity of Glycine wightii against Streptococcus mutans was also found. The protein content of Mimusops elengi seed protein extract is 5.84mg/ml. The Minimum Inhibitory Concentration (MIC) values for Staphylococcus aureus and Streptococcus pyogenes against Mimusops elengi seed protein extract are 364.36µg/ml and 182.19µg/ml respectively. Kinetic study further elucidates the mode of inhibition in the presence of the Mimusops elengi plant seed protein in context to time. The concentration of crude extract which gave 50% hemolysis compared to Triton X-100 treatment (HC50) value was 1.58 mg/ml which is more than five time larger than that of the MIC. Treatment with proteinase K of the Mimusops elengi seed protein results in absence of the inhibition zone which clearly indicates the activity was only due to protein. Our results showed the prominence of Mimusops elengi plant seed proteins as successful traditional pharmaceutical drug in practice. Keywords: Antimicrobial activity, Mimusops elengi, Hemolysis P-259: AQUASOMES: A NOVEL DRUG CARRIER Dhruv Desai, Nandu Menat Institute Of Pharmacy, Nirma University, Ahmedabad Aquasomes are nanoparticulate carrier system comprised of a solid phase nano-crystalline core coated with oligomeric film to which biochemically active molecules are adsorbed with or without modification. Firstly, aquasomes protect bio-actives. When Prodrugs and liposomes utilized in the process, they are prone to destructive interactions between drug and carrier in such case aquasomes proved to be worthy carrier. Aquasomes possess water like properties. It has a large size and active surface. Aquasomes are mainly characterized for structural analyses, particle size, and morphology. Aquasomes are used as red blood cell substitutes. Aquasomes used as vaccines for delivery of viral antigen and also been used for successful targeted intracellular gene therapy. Aquasomes are also used for delivery of enzymes. The general procedure for aquasomes preparation consists of an inorganic core formation, which will be coated with Lactose forming the polyhydroxylated core that finally will be loaded by model drug. These three layered structure are self assembled by non-covalent bonds. Principle of "self assembly of macromolecule" is governed by three physiochemical process: Interaction between charged group, Hydrogen bonding and dehydration effect, & Structural stability. Aquasomes represent one of the simplest yet a novel drug carrier based on the fundamental principle of self assembly & also a promising carrier for the delivery of a board range of conformational sensitive molecules with better biological activity. Keywords: Aquasomes P-260: PHYTOCHEMICAL STUDIES AND IMMUNOMODULATORY ACTIVITY OF ROOT BARK STREBLUS ASPER LOUR. Tijare RD, Becknal AK, Mahurkar N, Chandey V. T. John College of Pharmacy, Bangalore The root bark of Sreblus asper Lour (Moracea) commonly known as Shakhotaka is an important drug of Ayurveda possesses antipyretic, antidysentric and analgesic property. The phytochemical studies were carried out which showed the presence of phytosterol, saponins, flavonoids etc. The physical constants such as total ash (11-66%w/w), acid insoluble (3.66%w/w) and water insoluble ash (5.26%w/w), water soluble extractive (35.832%w/w), alcohol soluble extractive (18. 056%w/w) were determined for standardization. The effect of Shakhotaka on immune system was determined by carbon clearance test, cyclophosphamide induced netropenia, serum immunoglobulin and mice lethality test. Animals were divided as positive control, negative control, standard, alcohol and aqueous extracts in different dose level. The animal were treated with respective dose for 21 days and vaccinated on 7th and 17th day. Lastly all animals were challenged with bacterial culture which helps in determining serum immunoglobulin which prevents the mortality induced by bacterial culture.
In immune compromised module all groups are treated with respective doses such as levamisole (1-25 mg/kg) alcohol and aqueous extract in dose levels of 250 mg/Kg and 350 mg/Kg. On 1st and 3rd day after cyclophosphamide administration, extent of neutropenia was determined, where alcohol extract in high concentration showed the significant immuno-modulatory property. Alcohol and aqueous extracts in different dose level showed a significant immunomodulatory activity by significantly increasing the serum immunoglobulin means ultimately reducing the mortality. The same was also proved by significant increase in phagocytic index carbon clearance assay, significant protection against cyclophosphamide induced neutropenia. Hence it was concluded that Sreblus asper alcohol and aqueous extracts increases both humoral and cell immunity which will be useful to further studies. Keywords: Streblus asper, immunomodulatory activity, mice lethality test P-261: ETHOSOMES: A NOVEL DRUG DELIVERY SYSTEM Patel Urmi, Sanjeev Acharya, Vimal Kumar, Niyati Acharya Institute of Pharmacy, Nirma University, Ahmedabad The herbal extracts and phytochemicals are found to be extensively used nowadays for topical use due to their less side-effect. But one of the major problems associated with these extract are solubility and permeability. The skin acts as a major barrier for the drug to reach to the deep layers in order to show the pharmacological effect. Hence, an effective transdermal system been developed using ethanol in the higher concentration and is termed as ethosome. Ethosomes are non-invasive vesicular system composed of phospholipids, ethanol, propylene glycol and water. They are able to entrap drug molecule which may be hydrophilic, lipophilic or amphiphilic. It helps enhancing permeation of the drug through stratum corneum. This system helps in solving problems related to insolubility, instability and rapid degradation of herbal extract and phytochemicals by avoiding first pass metabolism. The system is useful for targeting various anti-tumor, anti-inflammatory drugs, hormones and various antibiotic peptide deliveries and also for cosmetics. The same can be used as an effective delivery system for herbal extracts and phytochemicals due to their solubility and permeability problems. Development of ethosomes of ammonium glycyrrhizinate, matrine and curcumin for its effective anti-inflammatory activity have already been reported. The ethosomes are capable to deliver the drug to the deep layers of the skin and can be used for treatment of various skin-disorders and also can be used to deliver the drug to the systemic circulation than conventional liposomes. Hence, it will provide a beneficial and effective delivery system for standardized herbal extracts and phytoconstituents with enhanced permeation. Drug can be administered by ethosomes in the form of gel and cream for patient comfort. This review focuses on various aspects of ethosomes like preparation, characterization, potential advantages and their applications in herbal based drug delivery. Keywords: Ethosomes, Herbal extracts, Drug-delivery, Permeation P-262: THERAPEUTIC POTENTIAL OF PHYTOALEXINS Shah Heta, Sanjeev Acharya, Vimal Kumar, Niyati Acharya Institute of Pharmacy, Nirma University, Ahmedabad Phytoalexins (low molecular weight phenolic antimicrobial substances) are not present in healthy plants but synthesized de novo upon stimulation by a pathogen, mechanical or chemical injury. They may puncture the cell wall, delay maturation, disrupt metabolism or prevent reproduction of the pathogen. When a plant cell recognizes particles from damaged cells or pathogen, it launches a two-distinct resistance: a short-term response (hypersensitive response due to free radicals) and a delayed long-term specific response (communication with rest of the plant with hormones like ethylene). Induction of phytoalexin genes is frequently transient, reaching peak in hours and then declining. Chlorogenic acid, Gossypol, Phaseolin, Rishitin, Pisatin etc are some examples of phytolaexins. Most of them are products of the shikimic acid pathway and few are of mevalonic acid pathways. Allixin (3-hydroxy-5-methoxy-6-methyl-2-penthyl-4H-pyran-4-one) is a phytoallexin found in garlic (Allium sativum) bulbs. When garlic is stored for 2 years the amount of allixin accumulated can approach 1% of the dry weight of the garlic. To study pharmacokinetic behavior, Allixin was administered in mice using an inclusion compound because the solubility of allixin in aqueous solution is very low. The allixin content in serum and in organs was analyzed by liquid chromatography (LC)-MS. Most of the administered allixin disappeared within 2 h, and bioavailability of allixin was estimated to be 31% by obtained area under the blood concentrationtime curve (AUC). Allixin was reported for its effects on aflatoxin B1 (AFB1)-induced mutagenesis using Salmonella typhimurium TA100.The findings indicate potential of allixin in the prevention of cancer. Resveratrol obtained from grape vine, is a phytoalexin produced under attack by pathogen, and sold as a nutritional supplement. It's antioxidant, anti-inflammatory and anticancer activity has been reported in mouse and rat experiments. Phytoalexins are potent therapeutic agents as antioxidant, antiinflammatory and anticancer in humans and as defense mechanism in plants. Keywords: Phytoalexins, Allixin, Antioxidant, Anti cancer, Resveratrol P-263: PHARMACOLOGICAL EVALUATION OF GMELINA ARBOREA FOR ITS ANTI CONVULSANT ACTIVITY Divyang Patel, Hiral Kataria, Vimal Kumar, Sanjeev Acharya, Niyati Acharya Institute of Pharmacy, Nirma University, Ahmedabad Epilepsy (or convulsion) is a neurological disorder characterised by unpredictable and periodic occurrence of a transient alteration of behavior due to the disordered, synchronous and rhythmic firing of populations of brain neurons. Gmelina arborea (Verbenaceae), is commonly known as shewan in Gujarati and Krishna in Sanskrit .
The root decoction is used in folk remedies for abdominal tumors, anasarca, anthrax, bilious disorder, blood disorder, cholera, convulsions, delirium, diarrhoea, dropsy, dyspepsia, epilepsy, fever, gout, The present study was aimed to evaluate anti convulsant activity of methanolic extract of roots & stems of Gmelina arborea by using two different animal model including pentylenetetrazole and strychnine induced seizure in mice. Methanolic extract of powdered stems revealed presence of flavonoids and phenolics. alkaloids, lignans, terpenoids, and phenylpropenes & they were also confirmed by thin layer chromatography. Both methanolic extract showed significant delay onset of convulsion. In pentylenetetrazole induced convulsion model, methanolic extract of stem in dose of 500 mg/kg showed maximum delay in onset of convulsion (8.188 min) and methanolic extract of root in dose of 500 mg/kg also showed remarkable increase in onset of convulsion (5.51 min). In case of strychnine induced convulsion model, methanolic extract of stem and methanolic extract of root at dose of 500 mg/kg found significant (P<0.001) as they delay onset of convulsion, 11.81 and 10.3 min respectively, which was lower than standard group (3.494 min). The results of these studies indicated usefulness of G.arborea in the treatment of epileptic disorders. Keywords: Epilepsy, Gmelina arborea, methanolic extracts P-264: A REVIEW OF PHYLLANTHUS AMARUS BASED ON ITS PHARMACOGNOSY, PHYTOCHEMISTRY AND PHARMACOLOGICAL USES Khyati Radadiya, Anu Patel, Karshan Bhetariya, Roopal Tanna Department of Pharmaceutical Sciences, Saurashtra University, Rajkot, Gujarat Herbal drug have been one of the primarily used drugs since olden time for the treatment of range of diseases. Bhumyamalaki (Phyllanthus amarus) belonging to the family Euphorbiaceae, is an important medicinal plant used against many of disorders. Phyllanthus amarus elaborates different classes of organic compounds of medicinal importance including alkaloids, flavonoids, steroids, terpenoids, lignans, lipids and coumarins. Because of its numerous medicinal properties, the plant is in great demand from both ayurvedic and pharmaceutical industries and needs commercial cultivation to meet its demand. Phyllanthus amarus,a world renowned botanical has been used since ages because of its rich medicinal values and ethanomedical importance. Phyllanthus amarus is a small erect, annual herb which is a rich source of phytochemicals that are attributed to biological active lignans, glycosides, alkaloids, ellagitannins and phenylpropanoids that are present in the leaf, stem and root of the plant. It is a little wonder that the plant is used for so many purposes in herble medicine systems and in clinical research over the years. Numerous studies have reported that phyllanthus amarus have anti-inflammatory, antidiabetic, antimicrobial, antihyperlidemic, antioxidant and anticancer activities. Moreover, studies have demonstrated hepatoprotective, antifertility, antidiarrhoeal, antiallodynic, antioedematogenic, antispasmodial, and chemoprotective, and antihypercalciuric, antiviral, antispasmodic, antinociceptive and diuretic properties associated with phyllanthus amarus. The present review is therefore, an effort to give a detailed study of Pharmacognosy, phytochemistry, traditional and pharmacological uses. Keywords: Phyllanthus amarus, Euphorbiaceae, Pharmacognosy, Pharmacology P-265: WOUND HEALING POTENTIAL OF "TYPHIN" Niyati Acharya, Kavita Mehta, Vimal Kumar, Sanjeev Acharya Department of Pharmacognosy, Institute of Pharmacy, Nirma University Plants belong to Typha genus have been mentioned in many traditional literatures of different countries but till date complete scientific study with respect to phytopharmacology has not been reported so far for much species. Many species growing in India and China have been reported to be used in traditional systems of medicine as a food and for the treatment of wounds, burns and ulcers. Looking to that the present study was designed to find and evaluate phytochemical and pharmacological profile of the species of Typha genus with special reference to wound healing activity. Various extracts aqueous extract, methanolic extract, ethyl acetate extract, chloroform extract and petroleum ether extract were prepared and preliminary phytochemical screening revealed presence of carbohydrates and alkaloids in all extracts, while aqueous, methanolic and ethyl acetate extract showed considerable presence of flavonoids, phenolics and tannins. `Typhin' is a flavonoids rich fraction prepared from the methanolic extract of the plant material. In present study 5 % ointment prepared from methanolic extract and typhin were applied in 1gm/day dose to evaluate the wound healing activity in excision and incision wound model. In excision wound model, wound healing activity was evaluated through measurement of % wound contraction and time of epithelization, determination of hydroxyproline and collagen content and histopathologically. In incision wound model activity was evaluated through tensile strength measurement of the wound. Significant wound healing activity was observed in the group treated with typhin as epithelization of the excised wound was faster with a high rate of wound contraction. Excision wound model results showed significant (98.505, p<0.05) % wound contraction on day 16 in the animals treated with typhin ointments as compared to control (62.19). The results showed significant (19.214, p<0.05) increase in tensile strength, in animals treated with typhin ointment when compared with the standard povidone iodine ointment. Keywords: Wound healing, typha, incision/excision wound, flavonoid fraction P-266: ANALYTICAL METHOD DEVELOPMENT AND EVALUATION OF EFFECT OF GERMINATION ON DIOSGENIN CONTENT IN FENUGREEK SEED BY HPTLC NK Patel*, KP Kotadiya, IS Rathod, NR Sheth, VB Airao, BD Patel *BK Mody Government Pharmacy College, Rajkot
Trigonella foenum graecum (Leguminosae) (Fenugreek seeds) is a plant growing in India, Europe, China, Iraq, Egypt, Spain, and Sudaan, Turkey. Diosgenin consider major chemical constituents of Fenugreek which is one of the important precursors for the synthesis of steroid hormone. Literature survey reveals that no systematic study published for estimation of diosgenin after germination of fenugreek seed. The method was validated as per ICH guidelines (CPMP/ICH/381/95; CPMP/ICH/281/95).Linearity, polynomial relationship was observed between concentration of analyte and the response with respect to the height and area of the peak in a range of 100-1000ng of Diosgenin. The correlation-coefficient found according to peak area and peak height is 0.9990 and 0.9996 respectively. The % C.V. for interday (n=7) was found to be 4.77-8.11 and 6.11-14.58 according to peak area and peak height respectively and intra-day precision (n=3). The % C.V. was found to be 1.36-6.61 and 1.56-5.23 according to peak area and peak height respectively. The repeatability in % C.V. was found to be 2.55 and 1.40 respectively with area and height. The repeatability of measurement in % C.V. was found to be 0.17 for peak area and 0.087 for peak height. The accuracy was determined by standard addition method and % recovery of the Diosgenin was found to be within 97.86% to 102.43%. The Limit of Quantification (LOQ) and Limit of Detection (LOD) were found to be 100ng and 50ng respectively. According to area, % w/w increase in Diosgenin content after germination was increase 44.35% whereas according to height % w/w increase in Diosgenin content after germination was increase 40.90%. Keywords: Diosgenin, HPTLC, Germination, Method development P-267: PHARMACOGNOSTIC EVALUATION OF PLUCHEA ARGUTA BOISS Yogeshkumar Vaghasiya2, Sumitra Chanda1 1 Dept of Biosciences, Saurashtra University; 2Dept of Biochemistry, Saurashtra University, Rajkot The main goal of pharmacognosy is to assess the value of raw materials and to ensure that the final product is of the required standard. Strict standardization procedures and pharmacognostical studies of medicinal plants would reduce drastically much of the accidents in wrong prescriptions of traditional herbal medicines. Yet alternative medicines based on plant substances are extremely popular, even though their safety and efficacy have not been scientifically proven. Nowadays routine pharmacognosy has changed demanding interdisciplinary research. In the present study, various pharmacognostic standards like botanical description, microscopy, ash values, extractive values, microscopic characteristics of powder, heavy metals, pH, solubility, microbial contamination and preliminary phytochemical study of Pluchea arguta were evaluated. This study could be useful for the compilation of a suitable monograph for proper identification of Pluchea arguta. P-268: OBESITY: A CONCERN ON HUMAN HEALTH AND ITS MECHANISM Devang Shelat, Shaival Shah, Vimal Kumar, Niyati Acharya, Sanjeev Acharya Institute of Pharmacy, Nirma University, Ahmedabad Obesity is an increasingly global problem, not only for the harm it causes in its own right but also due to the associated health threats like type II diabetes, systemic hypertension, cardiovascular disease, certain cancers, asthma, and sleep apnea. The obesity incidence has increased at an alarming rate in recent years becoming a worldwide health problem with incalculable social costs. Mostly synthetic drugs are used to treat obesity with two main mechanisms the inhibiting pancreatic lipase activity and suppression of appetite. The process still having various hazardous side-effects including increased blood pressure, dry mouth, constipation, headache, and insomnia. The approach is on a herbal side having plant containing of phytosterol are reported for antiobesity activity. Many herbal drugs are available to treat obesity showing a significant effects with various mechanism of action. Keywords: Obesity, Pancreatic Lipase, Herbs P-269: SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF OMEPRAZOLE AND ONDANSETRON FROM COMBINED TABLET DOSAGE FORM Padhiyar Miraba, Patel Poonam, Dedania Zarna, Dedania Ronak, Sheth Navin, Vadaliya K. R. Shree H. N. Shukla Institute of Pharmaceutical Education & Research, Rajkot Simple, accurate, economical and reproducible spectrophotometric method for simultaneous estimation for Omeprazole and Ondansetron in combined tablet dosage form has been developed. The developed method involves first order derivative spectroscopy, using zero crossing point of both drugs. Omeprazole showed zero crossing point at 249.09 nm while Ondansetron showed zero crossing at 226.91 nm. The method obeys Beer's law in the employed concentration ranges 4- 20 µg /ml for Omeprazole and Ondansetron. The equation of the calibration curve for Omeprazole and Ondansetron obtained was y = 0.0101x + 0.0191 and y = 0.0194x 0.0077 respectively and Correlation coefficient (r2) for Omeprazole and Ondansetron was found 0.9963 and 0.9971 respectively. Results of analysis were validated statistically and by recovery studies. The mean % Recovery was found to be 99.8 % ± 0.72 - 100.31 % ± 0.43 for Ondansetron and 99.16 % ± 1.25- 100.38 % ± 0.50 for Omeprazole. The RSD for all the parameter were found to be less than 5%. The method described can be employed for quality control and routine analysis of drugs in pharmaceutical preparations. Keywords: Spectrophotometric Estimation, Omeprazole, Ondansetron P-270: SOLASODINE: A NATURAL STEROID: A REVIEW Dhruv Ramanuj*, Punit Bhatt, Navin Sheth Department of Pharmaceutical Sciences, Saurashtra University, Rajkot
Many plants in the Solanaceae family, such as tomatoes, potatoes and eggplant, possess steroidal alkaloids based on a C27 cholestane skeleton, such as tomatidine and solasodine. These compounds are essentially nitrogen analogues of steroid saponins such as diosgenin, which is a precursor of steroidal hormones and antiinflammatory steroids. Solasodine is an aglycon portion of the solasonine-a glycoalkaloid. It is commonly found in solanaceae family like Solanum xanthocarpum, Solanum indicum etc. Solasodine is attached with sugar moieties by various linkages. Being a steroidal in nature now-a-days, it has been set target for the synthesizing new compounds as well also used for the various pharmacological action like as anti-inflammatory, anticancer etc. Solasodine already proves for its anti-inflammatory action at molecular level, and also in the cancer treatment. From physical properties point of view, It has molecular weight 413 Dalton and lipophilic in nature. Present review shows the various properties and various pharmacological action of Solasodine. Keywords: Solasodine, Natural product, Solanaceae, Steroid P-271: EFFECTIVE ROLE OF ACHYRANTHUS ASPERA SEED L. EXTRACT AND ITS ISOLATED CRUDE SAPONINS ON LIVER AND AORTA OF CHOLESTEROL TREATED RATS Ajay Kumar Sharma1*, Jyoti Nanda Sharma2, Harish Rajak2, Ashutosh2 and Vinod Kumar Dixit2 1Dept of Pharmacy, G.S.V.M. Medical College, Kanpur; SLT Inst. of Pharm. Sci., Guru Ghasidas Uni Hyperlipoproteinemias cause atherosclerosis, which is a major cause of death in the developed world and is also now becoming a major cause of morbidity not only in India but it has its tentacles globally. Ethanolic extract of seeds (100 and 200 mg/kg bodyweight) of Achyranthus aspera as well as saponins (10 mg/kg bodyweight) isolated from its seed were investigated for lipid lowering activity on triton induced and high fat diet induced model. Serum lipids were found to be lowered in both models and data were analysed statistically significant. In acute studies of triton induced model ethanolic seed extract (100 and 200 mg/kg bodyweight) decrease serum total cholesterol, triglyceride and LDL-cholesterol levels by 29.11% and 35.0%, 49.77% and 56.12%, 38.42% and 50.60% respectively. While isolated saponins (10 mg/kg bodyweight) decreases serum total cholesterol, triglyceride and LDL-cholesterol levels by31.66%, 51.65% and 44.41%, while HDL- level increased in ethanolic extract with above dose was 5.04% and 10.59% and in case of saponins it was found to be 8.29%. In chronic studies of high fat diet induced model Ethanolic extract of seeds (100 and 200 mg/kg bodyweight) decrease serum total cholesterol, triglyceride and LDL-cholesterol levels by 45.14% and 50.08%, 31.50% and 39.02%, 68.73% and 72.73%. In case of saponins (10 mg/kg bodyweight) decrease was 49.95%, 32.96% and 74.43%. Increase in the level of HDL-cholesterol in ethanolic extract (100 and 200 mg/kg bodyweight) and saponins (10 mg/kg bodyweight) was found to be 58.72%, 81.52% and 86.45% respectively. In morphological analysis the degenerative changes such as lobules with alteration in central vein, several foci of bridging necrosis, piecemeal necrosis with mono nuclear infilteration in liver and appearance of foam cells and atherosclerotic clot in aorta of hyperlipidemic rats treated with saponins (10 mg/kg bodyweight) when compared with hyperlipidemic groups was minimal or absent. HDL- raising property makes it promising cardio protective. Our study also revealed that the saponins are major contributor of the observed lipid lowering activity and thus may be suitable drug candidates worthy of pharmaceutical investigation. Keywords: Atherosclerosis, Hyperlipidemia, Triton induced, High fat diet induced P-272: EVALUATION OF HEPATOPROTECTIVE ACTIVITY OF LEAF OF LAUNAEA PROCUMBENS (ROXB.) Mukesh Kher*, Darshit Patel, Ketangiri Aparnathi, Ashvin Dudhrejiya, Navin Sheth Department of Pharmaceutical Sciences, Saurashtra University, Rajkot Launaea procumbens (Roxb.) Ram & Raj. (Family:Asteraceae) is a decumbent herb growing throughout India less or more as a weed.leaf juice of the launaea procumbens has been traditionally used for the treatment of liver disorders. Plant also used in skin disoders, fever, indigestion, diabetes, jaundice, cancer, urticaria, asthma, bronchitis, etc... but no scientific evidence has been established regarding the liver protective activity so, we undertake liver protective activity of ethanolic and aqueous extract of the leaf of Launaea procumbens(Roxb). The aqueous and ethanolic extracts prepared from the shade dried leaf of Launaea procumbens were evaluated for hepatoprotective activity in rats by inducing liver damage by carbon tetrachloride and silymarin (200mg/kg) was used as positive control. The ethanolic extract oral dose of 200mg/kg exhibited a significant (P < 0.05) protective effect by reducing serum levels of glutamic oxalo acetic transaminase, glutamic pyruvic transaminase alkaline phosphatase, total bilirubin and increasing the level of total protein and albumin levels but these two parameter was not significant as compared to toxic cotrol. The activity may be a result of the presence of phenolic compunds, triterpinoid and flavonoids compounds. Acute toxicity of the extracts showed no signs of toxicity up to dose level of 2000mg/kg. Thus concluded that ethanolic and aqueous extract of Launaea procumbens possess significant hepatoprotective activity. But result showed ethanolic extract was more potential liver protective activity than aqueos extract. Keywords: Launaea procumbence, Hepatoprotective, Ethanolic extract, silymarin P-273: VITAMIN A PROTECTIVE AGAINST ISOPROTERENOL INDUCED MYOCARDIAL INFARCTION Haresh J. Pipaliya, Jitendra D. Vaghasiya, Arindam Paul, Shyamsundar S. Pancholi Babaria Institute of Pharmacy, Vadodara, Gujarat Background/Aim: Vitamin A is known to help repair damage tissue and therefore may be beneficial in counter acting free radical damage. A great deal more research is needed that addresses the role of vitamin A in myocardial infarction and that is why present study design to investigate effect of vitamin A on myocardial infarction. Methods and Results: Myocardial infarction in rat was induced by isoproterenol (150mg/kg s.c.) at
the interval of 24 h for 2 days, which was evidenced by elevation of ST- segment. Vitamin A treated rats that underwent myocardial infarction demonstrated significant (P < 0.05) perfection in hemodynamic parameters. The infarct sizes were significantly (P < 0.05) reduced in vitamin A treated rats compared to non treated rats. Furthermore, myocardial infarction in vitamin A treated rats exhibited significant (P < 0.05) decrease in the serum concentrations of cardiac troponin - T, CK - MB, lactate dehydrogenase and creatinine kinase compared to infarction control rats. Vitamin A treated rats demonstrated significant restoration of membrane bound enzymes like Na+ - K+ ATPase, Ca2+ - ATPase and Mg2+ - ATPase compared to infarction control rats. Lipid peroxidation, myeloperoxidase activity and nitric oxide level in cardiac tissue were significantly (P < 0.05) decreased in vitamin A treated rats compared to infarction control rats. Antioxidant enzymes like glutathione, superoxide dismutase and catalase were significantly (P < 0.05) increased after myocardial infarction in vitamin A treated rats compared to non treated rats. Conclusions: In conclusions, vitamin A attenuated myocardial infarction, and the prevention being shown to achieve via nitric oxide generation and neutrophil sequestration in the cardiomyocytes. Keywords: vitamin A, myocardial infarction, infarct size, ATPase, oxidative stress P-274: PHYTOCHEMICAL INVESTIGATION AND ANALGESIC PROPERTIES OF HIBISCUS CANNABINUS Randive T. J., Shetty S. C., Kore K. J., Shete R.V. RDCOP, Bhor ,Pune Hibiscus cannabinus,of family Malvaceae plant is extensively used in traditional system of medicine as antioxidant, cardioprotective, antihypertensive and antiproliferative activities.. The plant is known to possess immunomodulatory effect. The literature survey revealed that there are no scientific studies carried out regarding analgesic activity. Hence the present study was performed to evaluate scientifically the analgesic activity of Hibiscus cannabinus. The shade dried powder of whole plant of Hibiscus cannabinus was subjected to successive extraction using solvents of increasing polarity. All the different extracts were tested for phytochemical investigation. Analgesic activity was studied using tail flick method in albino mice. The animals were divided into 4 groups of six animals each. Group-I received 0.2ml of normal saline and served as control, Group-II received 6.75mg/kg of body wt. of Diclofenac Sodium orally and served as reference standard. GroupIII and IV served as test and received 200 and 400mg/kg.of body wt. of dried ethanolic extracts respectively. The basal time was recorded at 0, 15, 30 and 60 minutes. Similarly the latency period was recorded in Acetic acid induced writhing test. From the result it was concluded that the Evolvulus alsinoides exhibited considerable analgesic activity by central and peripheral mechanism(s). P-275: EVALUATION OF ANTI-INFLAMMATORY AND ANALGESIC PROPERTIES OF EVOLVULUS ALSINOIDES Todkari N. T., Shetty S. C., Kore K. J.,Shete R.V. RDCOP, Bhor, Pune Evolvulus alsinoides, belonging to Convolvulaceae family commonly known as Shankhpushpi is used as braintonic in traditional system of medicines like Ayurveda and Unani. The plant is known to posses antibacterial, anticatatonic, and antiulcer. In the present study ethanolic extract of the entire herb of Evolvulus alsinoides was used to evaluate analgesic and anti-inflammatory activity. The result proved that Evolvulus alsinoides has considerable analgesic and anti-inflammatory activities. Keywords: Evolvulus alsinoides, Analgesic, Anti-inflammatory, Pleth P-276: DEVELOPMENT OF ECONOMICAL NUTRACEUTICAL PRODUCTS FOR RURAL POPULATION Urvashi V. Marakana*, Amol R. Pachade, Jyotsana R. Madan, Meera C. Singh, Kishore N. Gujar Sinhgad Technical Edu. Society's Smt. Kashibai Navale College of Pharmacy, Kondhwa (Bk), Pune Under nutrition, directly affects many aspects of children's development. Underweight prevalence is higher in rural areas than in urban areas. The present study is focused on development of economical Nutraceutical products for rural population. Through extensive literature survey, we found economical/affordable sources like Portulaca quadrifida, Aloe vera, Corn grains and Soyabean powder. Portulaca quadrifida is used as source of micronutrients and its prebiotic potential has been established through present study. It also has antimicrobial activity against food pathogens. Aloe vera gel extracted from Aloe barbadensis has been studied and found to stimulate the humoral immunity. Corn grains is excellent feed that is moderately high source of protein, low in starch, high in digestible fibre and low in oil. Soyabean powder is used as protein source. Liquorice powder is preferred to mask post-bitter taste of Aloe vera gel. The first series of the products are designed for malnutrition, are in two forms: Biscuit and powder. The product comprises of Maize (Corn grains), Corn calyx, Soyabean powder, Portulaca quadrifida, Aloe vera gel, Liquorice powder, Ghee, Jaggery (Mc SPALG). The estimated cost of product was found to be 29paisa/g. The second series of products designed for diabetic/obese patients is in the form of powder. This product does not include energy sources like ghee, jaggery. In conclusion the first series of products (Biscuit/powder) can help to resolve the problem of malnutrition in tribal pockets like Melghat/ Amravati (M.S.). The second series of product in the form of powder useful for diabetic/obese patients has potential benefit of immunomodulation, Prebiotic, Micronutrient and fibre values. Since all ingredients fall in GRAS category, it can be presumed to be safe as per regulatory status in India. Thus the present studies have helped to develop a promising `Economical Herbal Health Supplement' as OTC products for rural India.
Keywords: Malnutrition, Nutraceutical, Portulaca quadrifida, Aloe barbadensis P-277: ANTIHYPERTENSIVE ACTIVITY OF ACORUS CALAMUS ON EXPERIMENTALLY INDUCED HYPERTENSION Pinal K. Patel, Jitendra D. Vaghasiya, Arindam Paul, S. S. Pancholi Babaria Institute of Pharmacy, Vadodara, Gujarat Background/Aim: Acorus calamus having the calcium inhibitory effect and diuretic activity which may potentiate Na+ excretion in hypertension induced by occlusion of renal artery. Therefore this study was aimed investigate the effect of Acorus calamus on experimentally induced hypertension. Methods and Results: Hypertension in rats was induced by clamping the left renal artery for 4h by PVC coated bulldog clamp. At the end of experiment animal were anesthetized with ketamine (50 mg/kg). Carotid artery was cannulated which was connected to pressure transducer for estimation of BP. Ethyl acetate extract of Acorus calamus (EAAC) treated rats that underwent hypertension demonstrated significant (P < 0.05) lower mean atrial pressure compare to non treated rats indicated blood pressure lowering activity. EAAC treated rats that underwent hypertension demonstrated significant (P < 0.05) lower mean blood urea nitrogen and creatinine compare to non treated rats. Lipid peroxidation was significantly (P < 0.05) decreased, where as nitric oxide level in serum was significantly elevated in EAAC treated rats compared to disease control rats. Antioxidant enzymes like glutathione, superoxide dismutase and catalase were significantly (P < 0.05) increased in EAAC treated rats compared to non treated rats. Conclusions: In conclusions, EAAC attenuated experimentally induced hypertension via nitric oxide generation. Keywords: Acorus calamus, hypertension, nitric oxide P-278: EVALUATION OF ANTIBACTERIAL ACTIVITY OF PANCHAVALKAL EXTRACT AND GEL FORMULATION AGAINST MULTIPLE DRUG RESISTANT STRAINS OF S. AUREUS Mayur Kamble1 , Shruti Ashar1, Savita Jadhav2 1 Smt Kashibai Navale College Of Pharmacy, Pune; 2 Dept of Microbiology, D.Y. Patil Medical College Staphylococcus aureus causes a variety of infections, ranging from skin and soft tissue infections to life threatening endocarditis. Methicillin resistant Staphylococcus aureus (MRSA) is a major cause of nosocomial and community acquired infections. However widespread use has led to an increase in the number of Staphylococcus strains resistant to Clindamycin and other lincosamide antibiotics, which have been among the limited choice of antimicrobials effective against MRSA. There is concern about use of this antibiotic in the presence of Erythromycin resistance because of the possibility of induction of cross- resistance. Thus the focus is on development of empirical antimicrobial therapeutic strategies for Staphylococcal infections. Panchavalkal (PV), is a formulation reported to be an effective external antimicrobial agent in two standard texts of Ayurveda, viz. Charaka Samhita and Bhavaprakash Nighantu and also scientific literature. It is prepared from the equal amounts of powder of the barks of five avenue trees, viz four Ficus species, (F. bengalensis, F. glomerata, F. infectoria, F. religiosa); and Albizzia lebbeck. In the present study, we aimed to screen the alcoholic extract of PV for In vitro antibacterial activity against MRSA (clinical isolates reported to be resistant to Gentamicin, Norfloxacillin, Penicillin G, Benzyl Penicillin, and Cefotaxamine; susceptible to Clindamycin). The barks were collected from the five trees after identification and botanical authentification of the trees. The barks were evaluated as per Ayurvedic Pharmacopoeia of India and then powdered. The powder was evaluated and alcoholic extract prepared by Soxhlet extraction. Standardized extract was evaluated for the antimicrobial activity reported here. A number of gels, intended to be used topically in wound infections were formulated and the best was evaluated for antimicrobial activity against MRSA using cup plate (agar well-diffusion) technique. The herbal extract and gel were found to be effective. The results demonstrated that PV could represent a source of antimicrobial agents, for the control of MRSA wound infections. Keywords: Panchavalkal, MRSA, herbal gel P-279: NUTRACEUTICALS CURRENT MARKET STATUS & REGULATORY GUIDELINES Neetu Singh, Vaibhav Gohel Institute Of Pharmacy, Nirma University, Ahmedabad Nutraceutical, a combination of the words "nutrition" and "pharmaceutical", is a food or food product that reportedly provides health and medical benefits, including the prevention and treatment of disease. Nutraceuticals are classified into major 4 groups: Dietary Substances, Functional Foods, Medical Foods, and Pharmaceuticals Kambocha tea is used for Improve diet, relief from arthritis and menstrual cramps Noni (Morinda citrifolia) Relief blood pressure, muscle pain. India's nutritional supplement market is expected to more than double in the next four years at over Rs9, 500 crore. Total market for these products in India can go up to Rs. 17, 000 crore in the next four years. Currently, the domestic market for nutraceutical is around Rs. 4, 400 crore, which is just 0.9% of the total global business in the category. Despite the international movement within the industry, professional organizations, academia, and health regulatory agencies to add specific legal and scientific criterion to the definition and standards for nutraceuticals, within the United States the term is not regulated by FDA. The FDA still uses a blanket term of "dietary supplement" for all substances without distinguishing their efficacy, manufacturing process, supporting scientific research, and increased health benefits. In Canada, Health Canada regulates the functional foods and nutraceutical industry and the Canadian Food Inspection Agency Enforces these regulations. The Japanese scientific academic Community defined
functional food early in the 1980s. That is, functional foods are those that have three functions. Nutrition, Sensory function or sensory satisfaction, and Physiological. The Japanese Ministry of Health, Labor and Welfare (MHLW) set up `Foods for Specified Health Use' (FOSHU) in 1991 as a regulatory system to approve the statements made on food effecting on the human body. Keywords: Nutraceuticals P-280: ESTIMATION OF ASIATIC ACID IN GUM RESIN OF SHOREA ROBUSTA AND ITS VARIOUS MARKET FORMULATIONS Nagja Tripathi*, M.Naeem Devla, Niyati Acharya, Sanjeev Acharya, Vimal Kumar Institute of Pharmacy, Nirma University, Ahmedabad Shorea robusta belonging to family Dipterocarpaceae is a well known tree known for its gum resins which is traditionally reported for its wound healing activity. The gum resin oil of Shorea robusta is rich in oligomeric stilbenes and triterpenoids like asiatic acid, oleanane and ursane and is highly effective as wound healing agent . Thus a sensitive and accurate high-performance thin-layer chromatographic method was developed, validated and used for quantification of Asiatic acid in gum resin of Shorea robusta and its various market formulations. The extracts were separated on aluminum HPTLC plates coated with silica gel 60 F254 using Camag Applicator and twin trough glass chamber and Chloroform: Methanol (9:1 v/v) was used as mobile phase. Derivatization of the plates were done by dipping in vanillin sulphuric acid reagent and heating at 105ºC for 1 min and detected at 615 nm in Camag TLC scanner. Response was a linear function of Asiatic acid concentration in the range 50 - 350ng /µl. The limits of detection and limit of quantification for Asiatic acid were 25ng and 50 ng/spot respectively. Average recovery of Asiatic acid was 107.66% which showed that the method was free from interference from excipients present in the formulation. The established method enabled accurate, precise, and rapid analysis of Asiatic acid in various formulations. Keywords: Shorea robusta, Asiatic acid, HPTLC, quantification P-281: TOTAL PHENOLIC ESTIMATION AND EVALUATION OF ANTIOXIDANT ACTIVITY OF DILLENIA INDICA Dipal Gandhi*, Priti Mehta Institute of Pharmacy, Nirma University, Ahmedabad, Gujarat Dillenia indica (Dilleniaceae) commonly known as "MOTA KARMAL", widely distributed in Dang forest, Gujarat is used for the treatment various diseases like cancer, fever, inflammation etc. Leaves and bark of the plant has been used by tribal communities of this forest. Present work includes estimation of total phenolics and evaluation of antioxidant activity of Dillenia indica which may be primarily responsible for its beneficial properties. In existing study, we carried out an efficient record of the comparative In vitro antioxidant activity in methanolic extract of the selected parts (leaves and bark) of Dillenia indica. The total phenolic contents were estimated in terms of Gallic Acid Equivalent (GAE) by using the Folin-Ciocalteu method while free radical scavenging activity of methanolic extracts were evaluated by using DPPH (1, 1 -Diphenyl- 2 -Picryl Hydrazyl) radical quenching assay. A positive correlation was found between phenolic contents and antioxidant properties. The greater amount of phenolic compounds leads to more powerful radical scavenging effect as shown by methanolic extract of Dillenia pentagyna. These findings also indicate that polyphenolic components may have influence on antioxidant potential. Keywords: Dillenia indica, total phenolics, antioxidant activity P-282: PHYTOSOMES: A NOVEL DRUG DELIVERY SYSTEM FOR HERBAL MEDICINES Vaibhav Gohel, Dhruv Desai Institute Of Pharmacy, Nirma University, Ahmedabad Phytosomes are new revolution in herbal and plants extracts delivery system. Basically phytosomes are formed when active molecule or drug which binds to the phospholipids mainly phosphatidylcholine. Thus active ingredient is surrounded by phospholipids. Flavonoids are major compounds which are used as a phytosomes. They enhance the absorption of the active constituent from the skin. Generally the passage of the compounds takes place through interaction with cutaneous structure, which influences the release of the phytoconstituents. The rate of absorption from the complex is dramatically enhanced with out damaging the skin. Phytosomes preparation is done by non-conventional method. Phytosomes are prepared by reacting from 2 to 3 moles but preferably with one mole of natural or synthetic phospholipids, like phosphatidylcholine or phosphatidylethanolamine or phosphatidyiserine with one mole of active herbal component like flavenoids. Silybin Phytosome are antioxidant for Liver and skin & Ginkgo Phytosome from Ginkgo biloba Protects brain and vascular lining & also having Anti ageing properties, are some examples of marketed phytosomes preparations. Phytosome are advanced forms of herbal products that are better absorbed, utilized and as a result produce better results than conventional herbal extracts the increased bioavailability of the phytosome over the traditional herbal derivatives has been observed by pharmacokinetics studies. Phytosome will be given in small quantity and desired results can be achieved. Phytosome are widely used in cosmetics due to there more skin penetration and have a high lipid profile. Phytosomes are used as a medicament and have wide scope in cosmetology. Many areas of phytosome are to be revealed in future in the prospect of pharmaceutical application. Phytosomes forms a bridge between the conventional delivery system and novel drug delivery system.
Keywords: Phytosomes P-283: ASSESSMENT OF SOIL PROPERTIES & ELEMENTAL COMPOSITION OF SOIL OF AN ORCHARD LOCATED AT BICHOLIN-GOA Alphonso Priya, Bernadette Alphonso & Aparna Saraf The Institute of Science, Department of Botany, Elphinstone College, Mumbai The area under study comprises of an orchard in Bicholim Taluka, measuring 25 acres of land having a variety of fruit growing tress which include Anacardium Species, Mangifera Indica, Bambusa Species, Tectonagrandis, Bombaxciba, Garcina Choisey & more. Soil is an important abiotic component that aids in providing nourishment to the vegetation. The study was aimed to analyze soil composition which included moisture content, organic content, pH, texture of soil & Elemental Composition qualitatively & quantitatively by ICPAES analysis. The area which is under study is adjacent to a final dumpy mine, which is continuously causing detoriation of the soil & making it Infertile affecting the productivity of the fruit yield by the activities carried out by the company. The soil type is red alluvial soil having pH 7 & having a large number of elements which include Al, B, Ba, Ca, Cl, Co, Cr, Fe, K, La, Li, Mg, Mn, Na, P, S, Sr, V, Ti, Y, Yb, Zn, Zr, Mo, I, As which is found to be helpful for plant growth. The analysis was carried out on the final product also which showed the composition similar to that on the layer surface layer of the alluvial soil, which is due to the particles which are carried and deposited by the wind however the particles are also found to be deposited on the surface of the plant leaves which is also analyzed. Thus the study shows that due to the mining activity the over-all fruit yield has been decreased in the orchard. Keywords: Bicholim, Elemental composition, pH, Soil, Texture. P-284: COMPARATIVE STUDY OF ELEMENTAL PROFILE OF JUGLANS REGIA (WALNUT), PRUNUS DULCIS (ALMOND) AND ARACHIS HYPOGAEA (PEANUT) OF INDIA Zainab Bohra & Alphonso Priya Department of Biotechnology, Elphinstone College, Mumbai The objective of this study is to establish an elemental profile of Juglans regia, Prunus dulcis, Arachis hypogaea on a comparative analysis. It was found that the composition of walnut is quinines, oil, tannins, nuts contain essential fatty acids, including cis-linoleic and alfalinoleic, The kernels contain oil, mucilage, albumin, mineral, matter, cellulose & water. It is used for sore mouth and inflamed throat, it is also used to heal wounds, also used in skin diseases. The composition of almond is flavonoid oxidant, tocopherol, magnesium & calcium, copper, mono-saturated fatty acids. Its anti-oxidant prevent cancer. Almond also helps in preventing cancer, heart disease. Almond is good for skin, it also prevent osteophorosis, it helps in strenting the bones, teeth & muscles, Prevent diabetes, constipation, colon & rectal cancer. The composition of peanut is vitamin, protein, lipid, carbohydrate, fiber, mineral, vitamin, amino acids, salicylic acid, purines. These dry fruits play an important role in the diet and have important place in home remedial recipes. It is important to establish a comparative elemental study and hence a full scan of all the elements present in Juglans regia, Prunus dulcis, Arachis hypogaea was carried out and thus a quantitative and qualitive profile was established. Keywords: Elemental Composition, Juglans regia, Prunus dulcis, Arachis hypogaea, recipies P-286: FORMULATION OF SOLID ORAL DOSAGE FORM OF ETHANOLIC EXTRACT OF BOERHAAVIA DIFFUSA L. ROOTS Ishwari N. Singh, Khemraj Bairwa, Amit K. Srivastava, Sanjay M. Jachak Department of Natural Products, NIPER, Mohali Introduction: Ethanolic extract of B. diffusa roots is reported to posses various biological activities but so far there is no report on its preformulation and formulation studies. Objectives: Tablet formulation of standardized ethanolic extract of B. diffusa roots and its evaluation for various pharmaceutical parameters as per Indian Pharmacopoeia (IP). Materials and Methods: B. diffusa roots were extracted with 70% ethanol by maceration at room temperature and lyophilized after removal of ethanol. The extract was standardized with respect to total flavonoids content (quercetin equivalents) and marker constituent, boeravinone B content by HPLC method. Compatibility study of different ratio of standardized extract and excipients blends were done under isothermal condition at 40°C±2°C/75%±5% RH for 30 days. Preformulation studies of extract were done for hygroscopicity (Callahans method), solubility, particle size by zeta sizer, flow property (angle of repose, tapped and bulk density, carr's index, housner ratio). The tablet formulation was optimized by various proportions of excipients and was evaluated for its pharmaceutical parameters viz. weight variation, hardness, friability, disintegration time and dissolution. Results and Discussion: The content of boeravinone B in the ethanolic extract was found to be within permissible IP limit (NLT 0.005% w/w). Excipients PVP, Aerosil, MCC, Mg stearate, and cross carmellose sodium showed no incompatibility with the studied extract. Preformulation parameters of extractexcipients blend and formulation parameters of tablets were within permitted limits as per IP 2010. Conclusion and Future Plan: The ethanolic extract of B. diffusa was successfully formulated as tablets and all parameters were within permissible limits. To the best of our knowledge this is the first report on tablet formulation of B. diffusa. Accelerated stability study of tablets (as per ICH guidelines) is currently under progress. Keywords: Boerhaavia diffusa, Formulation, Standardization, Evaluation
the health and the effectiveness of the treatments. For example, some herbal therapies might Increase the side effect of drugs, possibly leading to toxicity, decrease the drug therapeutic effects, possibly leading to the treatment failure, modify the actions of drugs, leading to unexpected complications,Enhance the therapeutic effect of drugs, leading to over medication etc.The clinical importance of herb-drug interaction depends on many factors associated with the particular herb, drug and patient. Keywords: Herbal therapies, Herb-drug interaction P-293: COMPARISION OF PHENOLIC CONTENTS AND IN-VITRO ANTI-OXIDANT ACTIVITY OF VARIOUS EXTRACT OF ROOT FROM ANISOMELES INDICA LINN P. Niveditha, Suva Pradeep, N. Bhaghya lakshmi, Pingale Prashant l SVKM'S NMIMS, School of pharmacy & technology management, Shirpur Anisomeles indica (Linn) is a plant used in folk medicine as a cure in gastric catarrh and intermittent fever. The antioxidant activity of the plant extract was studied because of presence of phenolic compounds. Methanolic, 50% hydro-alcoholic and aqueous extract of Anisomeles indica was prepared and standardized to their phenolic content. The total phenolic content of the extract was estimated using Folin- Ciocalteau's reagent. The antioxidant activity of plant extracts were measured by in vitro methods such as 1, 1 diphenyl 2 - picryl hydrazyl (DPPH) and nitric oxide (NO) scavenging assay using spectrophotometer. The result indicated that the higher phenolic content and antioxidant activity was found in methanolic extract of Anisomeles indica. The present study under investigation showed a positive co-relation between phenolic content of the extracts and their antioxidant activity. To conclude, the correlation of the total phenolic content of the extracts and their antioxidant activities was found to be significant. Keywords: Folk medicine, catarrh, intermittent fever, antioxidant, scavenging. P-294: ISOLATION AND STANDARDIZATION OF UREASE FROM JACK BEAN N.Bhaghya lakshmi, P.Niveditha, Bhalani.Vikas, S. B. Bagade SVKM's NMIMS, School of Pharmacy & Technology Management, Shirpur Campus. Canavalia ensiformis, commonly called as Jack-bean, is a legume which is used for human nutrition and animal fodder is used in isolation and standardization of enzyme urease. C. ensiformis contains large quantities of the enzyme urease, for this reason the bean has been investigated as a potential source of the urease enzyme. The Jack bean appeared to be extraordinarily rich in urease. It was found that extraction with 30 percent alcohol was of distinct advantage, in as much as this solvent dissolved most of the urease but failed to dissolve a rather large quantity of the other proteins. Hence a considerable purification was achieved through the use of this solvent. The only disadvantage of 30 per cent alcohol lay in the slow inactivating action of this solvent upon the urease. However, if kept at low temperatures there was no inactivation of the enzyme. When kept at low temperatures 30 per cent alcoholic extracts of jack bean meal formed precipitates. These precipitates contained practically all of the urease, together with concanavalin-A, concanavalin B and other proteins. Enzyme may separate from solution as spheroids. This indicates a tendency to crystallize; Spheroids are nearer the crystalline state. Keywords: Urease, C. ensiformis, Spheroids, Jack Bean, Concanavalin
P-295: ANTI BACTERIAL ACTIVITY OF SEAWEED EXTRACTS AGAINST PATHOGENIC BACTERIA Yogesh Paneliya, Mahesh Sorathiya, Yogeshkumar Vaghasiya Department of Biochemistry, Saurashtra University, Rajkot Marine algae are not only the primary and major producers of organic matter in the sea, but they also exert profound effects on the density and distribution of other inhabitants of the marine environment. The present study was conducted to compare the antimicrobial activity of different extracts (acetone, methanol, chloroform, diethyl ether, ethy acetate, hexane and aqueous) of three seaweed species (Sargassum meriffidii, Halymenia species, Ulva lactua). Seaweeds were collected from okha north east cost of Gujarat, India. Extracts were tested in vitro for their antibacterial activities against S. epidermis, E. coli, B. circulans, Mycobacterium smegmatis, Micrococcus luteus, B. subtilis by the disc diffusion method. Acetone extracts were potential as antibacterial agents. Seaweed extract of Sargassum was found to be effective against Gram negative and Gram positive bacteria Results showed higher antibacterial activity were found in Ulva lactua and minimum found in Halymenia species. Further studies are required for phytochemical analysis of seaweeds as a new source for antimicrobial properties. P-296: PHYTO -TOXICOLOGICAL STUDY OF CERTAIN PLANTS IN JAMBUDIA VIDI SAURASHTRA REGION(INDIA) Smt.Reena P. Dave, Dr. R.S. Patel Dept. of Botany, ShriM.N.Virani Science College Rajkot 360 005, KKSJ Maninagar Science College Ahmedabad
With a wide variety of physical features and climatic conditions,India Possesses the richest and perhaps most diversified flora of all other Countries of a similar size on the surface of the earth, It is estimated that out of 250,000 300,000 total plant species of the world, India harbors about 45,000 plant species (Singh 2000).The diverse Vegetation and multiethnic groups make India one of the richest countries in term of culture and bio resources having all kind of food, Fiber, timber, species, gums, oils, Perfumes and medicinal plants. The country is also rich in population of poisonous plants but the official records are very less. Plants that causes undesirable or harmful effects on humans and animals are called poisonous plants, some plants shows their toxicological effect after ingestion whereas others only require contact to elicit response in human and livestock Knowledge of on poisonous plant is important as some of them are used in medicine. The Poisonous properties are due to toxic substances such as alkaloids, glycosides, saponins, tannin, resins, amines and bitter principles, etc. Jambudiavidi is Located between 22°, 29°, 69° N and 70°, 79°,84° W longitudes, in WankanerTaluka of Rajkot district in saurashtra peninsula. The northern part of Rajkot district adjoing to surendranagar district.A variety of soil is met with in they are, red soil, Loamy Soil, Lateritic and Patches of black cotton Soil Which are compact and grayish green and black in color are common the Climate of the region is tropical and semi-arid characterized by hot summer dryness in the non-rainy season and short monsoon. A wide variation occurs in temperature from season to season which rises form 40°- 41°C and coldest day 10°C. Winds are generally lite too moderate. But summer and monsoon may become stronger.A lot of work has been done on the vegetation of saurashtra region but no work has been done specifically on Phyto toxicological study of JamudiaVidi at saurashtra region. The present study is based on Self Observation in the actual fields and queries with Local Villagers and rural people residing in remote and far-Flung area of the district surveys were made in such a way that the utility of each and every poisonous plant growing n different seasons in different localities should be recorded. An account of 25 poisonous plant species has been gathered from the rural people of jambudia and surrounding villages namely Lunsar, Jivapar, Chitrakhada and Rajagadh. In this present work a brief account of toxicological plant resources being utilized by the farmers in remote Communities and other rural people in multipurpose for their exploitation in preparation of herbal products.