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AESTHETIC AND BIOLOGICAL FACIAL REJUVENATION TREATMENT WITH PLATELET-RICH PLASMA:AN ALTERNATIVE TO PHOTOLIGHT RESURFACING Donald Du Toit PHD., FRCS and Nardus Le Grange ,Photolight Laboratory, University of Stellenbosch and Medical Research Council, September/October 2007

BIOLOGICAL REJUVENATION BASED ON SCIENCE:SECRETORY PROTEINS IN PRP

PDGF (CE marked) TGF IGF EGF VEGF FIBRONECTIN

HISTOLOGY

PRP-ACR

SHIFT HEALING CASCADE TO LEFT

PRP

COLLECTION KITS

Du Toit et al IJSS 1:34, 2007

Platelet-rich Plasma Kit and Collection tubes: available in RSA

PLATELET RICH PLASMA

Centrifuge 3000 rpm

Wright's Stain X 40

PDGF, TGF, IGF, EGF CELL STIMULATION: ETHICS 42:2005

FIBROBLASTS EX VIVO

Keratinocytes

Du Toit et al IJSS 1, 34-43, 2007

ACR AMELIORATION OF THE SOLAR AGED FACE ( 59 Years)

Biological fIller by GF stimulation: Technique of injection (Topical anaesthetic Cream)

AUTOLOGOUS PRP MESOTHERAPY BY INTRADERMAL GROWTH FACTORS

NEW ADVANCE

ANATOMICAL AND CELL BIOLOGY CONSIDERATIONS

SKIN ANALYSIS,TEXTURE,COLOUR,SECRETIONS

EX VIVO VALIDATION

NERVES OF UPPER LID

VASCULATURE

PRP ACR FACIAL REJUVENATION: BEFORE AND AFTER 10 , 21 DAYS (45 years)

Down time minimal: safe

PRP ACR FACIAL REJUVENATION: BEFORE AND AFTER 3 WEEKS, CYCLE PHOTODAMAGE, 59 YEARS

PHOTOAGED SUN PHOTO-AGED MALE FACE: THICKER DERMIS

Bionic Serum

PRP ACR FACIAL REJUVENATION: BEFORE AND 21 DAYS ( NO MAKE-UP): 59 YEARS, SMOKER

Genetics, cigarettes POST MENOPAUSAL, ACTINIC DAMAGE, TOBACCO

COLLAGEN VERSUS ELASTIN FIBERS: FIBROBLAST STIMULATION BY GF

Sebaceous gland

Elastin fibers

V e Elastin Stain

VERHOEF

HISTOLOGICAL SKIN ANALYSIS: Du Toit et al 2007

REGGIE COLLAGEN

Follow-up 18 months: can add RF, IPL or Frac Laser

MEASSURED OUTCOMES: ACUTE END-POINT ANALYSIS

Subjective client satisfaction: Objective Photography (B +A): Skin analysis ( texture, colour, secretions):SD 202, C+K: Dermascan (U/S): Classification: Fitzpatrick, Glocoau: -

1. 2. 3. 4.

THEORETICAL ADVANTAGES OF ACR AND PRP

Autologous Safety profile ( Japan, England, Germany, Switzerland.... Plastic surgeons) Avoids post-inflammatory hyperpigmentation of lasers and IPL Avoids 2D pulled face of face lift , gives plumped 3D configuration, esp forehead, jowls, neck and hands Avoids scarification of lasers Does not address midface ptosis and severe jowls or telangiectasis

Thank you

Stellenbosch Campus Your knowledge partner

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AESTHETIC AND BIOLOGICAL FACIAL REJUVENATION TREATMENT WITH REGENLAB PRP:AN ALTERNATIVE TO PHOTOLIGHT RESURFACING

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