Read Phenylmethanesulfonyl fluoride (P7626) - Product Information Sheet text version

PHENYLMETHYLSULFONYL FLUORIDE Product Number P 7626 Storage Temperature RT CAS #: 329-98-6 Synonyms: PMSF; -toluenesulfonyl fluoride Product Description Appearance: White to faint yellow powder Molecular weight: 174.2 Molecular Formula: C7H7FO2S 1 Melting Point: 91-92°C Sigma typically finds values in the range of 91-94°C. PMSF is a inhibitor of serine proteases and 2 acetylcholinesterase. It inhibits proteases such as chymotrypsin, trypsin, thrombin and thiol proteases such as papain. PMSF inhibits serine proteases by sulfonating serine 3,4 residues at the active site. Precautions and Disclaimer PMSF is considered a highly toxic cholinesterase inhibitor. See Material Safety Data Sheet. Preparation Instructions PMSF is soluble in anhydrous isopropanol at 35 mg/ml with heating, resulting in a clear to very slightly hazy, colorless to faint yellow solution.


Recommended stock solution: 100 mM in anhydrous isopropanol or anhydrous (100%, not 95%) ethanol. A 200 mM solution in dry solvent is stable for at least 9 5 months at 2-8°C. PMSF is very unstable in the presence of water. The half-life of aqueous PMSF at 25°C at pH 7.0, 7.5, and 8.0 is 110, 55, and 35 minutes, 6 respectively. Storage/Stability When stored as indicated, PMSF has a shelf-life of three years. References 1. J. Am. Chem. Soc., vol. 85, 997 (1963). 2. Turini, P., J. Pharmacol. Exp. Ther., vol. 167, 98 (1969). 3. Gold, A.,Methods in Enzymology, vol. 11, 706 (1967). 4. Gold, A. and Fahrney, D., Biochemistry, vol. 3, 783 (1964). 5. Proteolytic Enzymes: A Practical Approach, edited by R.J. Benyon, page 246 (1989). 6. Anal. Biochem., vol. 86, 574 (1978).

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Phenylmethanesulfonyl fluoride (P7626) - Product Information Sheet

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