Read Lectin from Glycine max (soybean) (L1395) - Product Information Sheet text version

Lectin from Glycine max Product Number L 1395 Storage Temperature 2-8 °C Product Description Lectins are proteins or glycoproteins of non-immune origin that agglutinate cells and/or precipitate complex carbohydrates. Lectins are capable of binding 1 glycoproteins even in presence of various detergents. The agglutination activity of these highly specific carbohydrate-binding molecules is usually inhibited by a simple monosaccharide, but for some lectins, di, tri, and even polysaccharides are required. Lectins are isolated from a wide variety of natural sources, including seeds, plant roots and bark, fungi, bacteria, seaweed and sponges, mollusks, fish eggs, body fluids of invertebrates and lower vertebrates, and from mammalian cell membranes. The precise physiological role of lectins in nature is still unknown, but they have proved to be very valuable in a wide variety of applications in vitro, including: 1. blood grouping and erythrocyte polyagglutination studies. 2. mitogenic stimulation of lymphocytes. 3. lymphocyte subpopulation studies. 4. fractionation of cells and other particles. 5. histochemical studies of normal and pathological conditions. Sigma offers a range of lectins suitable for the above applications. Most Sigma lectins are highly purified by affinity chromatography, but some are offered as purified or partially purified lectins, suitable for specific applications. Many of the lectins are available conjugated to (conjugation does not alter the specificity of the lectin): 1. fluorochromes (for detection by fluorimetry). 2. enzymes (for enzyme-linked assays). 3. insoluble matrices (for use as affinity media). Please refer to the table for general information on the most common lectins. This lectin reacts stronger with type A1 red blood cells that with A2 red blood cells and much more than with type B red blood cells (A1>A2>>B). This product can be used in formation of polymeric lectin with enhanced hemagglutination and mitogenic activity toward neuraminidase-treated lymphocytes 2 from mice. Precautions and Disclaimer For Laboratory Use Only. Not for drug, household or other uses. Preparation Instructions This lectin is soluble in phosphate buffered saline, pH 7.2 (1 mg/ml).

Lectin Abrus precatorius Agglutinin Abrin A (toxin) Abrin B (toxin) Agarius bisporus Anguilla anguilla Arachis hypogaea Artocarpus integrifolia Bandeiraea simplicifolia BS-I BS-I-A4 BS-I-B4 BS-II Bauhinia purpurea Caragana arborescens Cicer arietinum Codium fragile Concanavalin A Succinyl-Concanavalin A Cytisus scoparius Datura stramonium Dolichos biflorus Erythrina corallodendron Erythrina cristagalli Euonymus europaeus Galanthus nivalis Glycine max Helix aspersa Helix pomatia Lathyrus odoratus Lens culinaris Limulus polyphemus Bacterial agglutinin Lycopersicon esculentum Maackia amurensis Maclura pomifera Momordica charantia Naja mocambique mocambique Naja naja kaouthia Narcissus pseudonarcissus Perseau americana Phaseolus coccineus Phaseolus limensis

MW (kDa) 134 60 63.8 58.5 40 120 42 114 114 114 113 195 a 60; 120 44 60 102 51 - 86 140 60 56.8 166 52 110 79 79 40-43 49 400 - 71 130 40-43 115-129 - - 26 - 112 247(II) 124(III)

Subunits 4 2 2() - 2 4 4 4 4 4 4 4 2/4 2 4 4 2 - 2() 4 2 2() 4() 4 4 - 6 4() 2 18 - - 2() 2() 4() - - 2 - 4 8 4

Specificity Blood Group Sugar - gal gal gal - -gal(13)galNAc H -L-Fuc T -gal(13)galNAc T -galOMe A, B A B acq, B, Tk, T - - - - - - - - A1 - - B, H (h) - A A - - - - - O - - - - (h) - - A -gal, -galNAc -galNAc -gal glcNAc -gal(13)galNAc galNAc fetuin galNAc -man, -glc -man, -glc galNAc, gal (glcNAc)2 -galNAc -gal(14)glcNAc -gal(14)glcNAc -gal(13)gal non-reduc. -man galNAc galNAc galNAc -man -man NeuNAc galNAc, glcNAc (glcNAc)3 sialic acid -gal, -galNAc gal, galNAc - - -D-man - - galNAc

Mitogenic Activity +

+

+

+ b +

+ + +

c

+ +

+

+

Phaseolus vulgaris PHA-E 128 4 - oligosaccharide + PHA-L 128 4 - oligosaccharide + PHA-P PHA-M - - - - - - - - - - - - - - - - - - - - - - - - - - Table continued on next page - - - - - - - - - - - - - - - - - - - - - - - - - -

Lectin Phytolacca americana Pisum sativum Pseudomonas aeruginosa PA-I Psophocarpus tetragonolobus Ptilota plumosa Ricinus communis Toxin, RCA60 Toxin, RCA120 Sambucus nigra Solanum tuberosum Sophora japonica Tetragonolobus purpureas

MW (kDa) 32 49 13-13.7 35 65; 170 60 120 140 50; 100 133 120(A) 58(BA) 117(C) 36 68 68 50 40 139 134 143 160 115 68

a

Subunits - 4() - 1 - 2 4 4() 1, 2 4 4 2 4 2 - - 4() 4() 4 4 4 4 4() 2

Specificity Blood Group Sugar - (glcNAc)3 - -man - gal - galNAc, gal B -gal - - - - A, B H H H - H - - - A1+Tn A1 Tn - - - galNAc, -gal -gal NeuNAC(26)gal galNAc (glcNAc)3 -galNAc -L-fuc -L-fuc -L-fuc (glcNAc)2, NeuNAc -L-fuc (glcNAc)2 man, glc glc, man galNAc galNAc galNAc -gal -gal galNAc

Mitogenic Activity + + c +

+

c

Triticum vulgaris Ulex europaeus UEA I UEA II Vicia faba Vicia sativa Vicia villosa A4 B4 Vigna radiata Viscum album Wisteria floribunda

a b c

+

+ +

Concentration-dependent molecular weight Non-agglutinating and mitogenic Mitogenic for neuraminidase-treated lymphocytes 2. Lotan, R., et al., Biochem. Biophys. Res. Comm., 55, 1347-1355 (1973).

References 1. Rueben, L., et al., Activities of lectins and their immobilized derivatives in detergent solutions. Implications on the use of lectin affinity chromatography for the purification of membrane glycoproteins. Biochemistry, 16, 1787-1794 (1977).

IRB/MWM/JRC/NSB/SAG 11/02

Sigma brand products are sold through Sigma-Aldrich, Inc. Sigma-Aldrich, Inc. warrants that its products conform to the information contained in this and other Sigma-Aldrich publications. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see reverse side of the invoice or packing slip.

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